pycom12g11690

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002522
Physical Location & Seq
Reverse (-)
14030831 .. 14032201
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11690.1

Sequence Viewer

Length: 969 bp
ATGACTGGTGGGCTATTAGAGAGGCTTATCGAAGAGTGTTCTAGTGAGGAAGGATCTGCTTGTGCCTTGCAACTTCATGGTGTACCCGGGGGAGCTAAAGCGTTTGAGCTTGTAACCAAATTTTGCTATGGCGTCAAAATAGAACTCACTGCTTTGAACGTAGTCATCCTTAGATGTGCAATTTTCCTTACTGAAGTCTTCAGCAATTGGAGAGACTCCATAGTAGCTCTTGAAACATGTGAAGATGTTCAACAATATGCTGAAGAGCTCCACATTGCTTCACGATGCATCGATTCCTTGGCTATCAAAGCTTGTGCAGATCCAAAGTTTATCAGTTGGCCTGTAGTATCAGGAGAAAATGACATGAAGAACCCAACAGCCTTATGGAACGGAATAACTTCTGATGACGCTAAGCCACAACCTCTCGCTGATGATTGGTGGTGTGAAGATGCATCCTTACTTAGCTTACCTCTCTATAAGCGATTGATTCAAGCTGTTGAATTGAAAGGAATGAGGCCTGAGAGCATTGCCTCGAGCTTTAATGACACAAATTATGTAAACTCGGGCACAACCATTTCTAACATTTCTGAGGCAGACCAGAGGGCTCTACTTGAAGATATTGTGGGGTTACTTCCTCATGAGAAAGGAGTCGCATCCTCCAAGTTTCTGCTTAGGCTGCTACAAACGGCTATGATCTTTCATGCAAGTCCGTCATGCAGAGAAAATTTGGAGAAAAGGGTAGGTGCCCAGTTGGACCAAGCTGTGCTCATAGATCTTCTCTTACCAAATATGGGCTATTCTTTGGAGACTCTTTACGACATAGACTGCATTCAGAGGATGCTCGATCATTTCATGTCAATAAACCAGGCTTCACTTTCACTTGCTTCCGTCATCCCTGAATATGCCCGGCTGCTGGATGATGGCATATATCATGCGATCAATGTTTTCTTGAAGGTAAAACAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

323

Amino Acids

35.63

Weight (kDa)

4.74

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 145 - 287 6.8e-40 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 743
AclWI GGATC 2 cut(s) 61, 314
AcsI RAATTY 2 cut(s) 119, 724
AcuI CTGAAG 3 cut(s) 184, 213, 282
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 2 cut(s) 791, 913
AflIII ACRYGT 1 cut(s) 236
AgsI TTSAA 8 cut(s) 157, 233, 251, 491, 500, 505, 614, 952
AjnI CCWGG 1 cut(s) 864
AluBI AGCT 9 cut(s) 95, 109, 227, 268, 311, 465, 494, 537, 761
AluI AGCT 9 cut(s) 95, 109, 227, 268, 311, 465, 494, 537, 761
Alw21I GWGCWC 2 cut(s) 270, 768
Alw26I GTCTC 2 cut(s) 207, 800
AlwI GGATC 2 cut(s) 61, 314
Ama87I CYCGRG 3 cut(s) 86, 532, 562
AoxI GGCC 2 cut(s) 338, 515
ApeKI GCWGC 2 cut(s) 676, 910
ApoI RAATTY 2 cut(s) 119, 724
Asp700I GAANNNNTTC 2 cut(s) 246, 397
AspS9I GGNCC 1 cut(s) 754
AsuC2I CCSGG 3 cut(s) 87, 88, 907
AvaI CYCGRG 3 cut(s) 86, 532, 562
AvaII GGWCC 1 cut(s) 754
BaeGI GKGCMC 2 cut(s) 569, 748
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 2 cut(s) 270, 607
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 2 cut(s) 270, 768
BbvI GCAGC 2 cut(s) 663, 897
BccI CCATC 1 cut(s) 914
BceAI ACGGC 1 cut(s) 702
BciT130I CCWGG 1 cut(s) 866
BcnI CCSGG 3 cut(s) 87, 88, 907
BcoDI GTCTC 2 cut(s) 207, 800
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 342
BglII AGATCT 1 cut(s) 772
BisI GCNGC 2 cut(s) 677, 911
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 2 cut(s) 678, 912
Bme1390I CCNGG 4 cut(s) 87, 88, 866, 907
Bme18I GGWCC 1 cut(s) 754
BmeT110I CYCGRG 3 cut(s) 86, 532, 562
BmgT120I GGNCC 1 cut(s) 754
BmiI GGNNCC 1 cut(s) 745
BmrFI CCNGG 4 cut(s) 87, 88, 866, 907
BmrI ACTGGG 1 cut(s) 742
BmsI GCATC 6 cut(s) 275, 297, 439, 461, 662, 828
BmuI ACTGGG 1 cut(s) 742
BpiI GAAGAC 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 671
Bpu1102I GCTNAGC 1 cut(s) 411
BpuMI CCSGG 3 cut(s) 87, 88, 907
Bsa29I ATCGAT 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 86, 87, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 791, 913
Bse1I ACTGG 2 cut(s) 10, 748
Bse3DI GCAATG 2 cut(s) 273, 525
BseBI CCWGG 1 cut(s) 866
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 86, 87, 297
BseGI GGATG 6 cut(s) 165, 452, 653, 843, 891, 922
BseLI CCNNNNNNNGG 2 cut(s) 791, 913
BseMI GCAATG 2 cut(s) 273, 525
BseMII CTCAG 2 cut(s) 510, 579
BseNI ACTGG 2 cut(s) 10, 748
BseSI GKGCMC 2 cut(s) 569, 748
BseXI GCAGC 2 cut(s) 663, 897
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 2 cut(s) 340, 517
BshNI GGYRCC 1 cut(s) 743
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 2 cut(s) 270, 768
BsiHKCI CYCGRG 3 cut(s) 86, 532, 562
BsiSI CCGG 2 cut(s) 87, 907
BslI CCNNNNNNNGG 2 cut(s) 791, 913
BsmAI GTCTC 2 cut(s) 207, 800
BsmI GAATGC 1 cut(s) 828
BsnI GGCC 2 cut(s) 340, 517
BsoBI CYCGRG 3 cut(s) 86, 532, 562
Bsp1286I GDGCHC 5 cut(s) 270, 569, 607, 748, 768
Bsp143I GATC 6 cut(s) 53, 319, 693, 772, 844, 936
Bsp1720I GCTNAGC 1 cut(s) 411
BspANI GGCC 2 cut(s) 340, 517
BspCNI CTCAG 2 cut(s) 511, 580
BspDI ATCGAT 1 cut(s) 291
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 1 cut(s) 745
BspPI GGATC 2 cut(s) 61, 314
BspQI GCTCTTC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 743
BsrDI GCAATG 2 cut(s) 273, 525
BsrI ACTGG 2 cut(s) 10, 748
BssECI CCNNGG 3 cut(s) 86, 87, 297
BssMI GATC 6 cut(s) 53, 319, 693, 772, 844, 936
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 866
Bst6I CTCTTC 2 cut(s) 27, 258
BstACI GRCGYC 1 cut(s) 132
BstDEI CTNAG 6 cut(s) 170, 411, 461, 519, 588, 671
BstF5I GGATG 6 cut(s) 165, 452, 653, 843, 891, 922
BstKTI GATC 6 cut(s) 56, 322, 696, 775, 847, 939
BstMAI GTCTC 2 cut(s) 207, 800
BstMBI GATC 6 cut(s) 53, 319, 693, 772, 844, 936
BstMWI GCNNNNNNNGC 2 cut(s) 308, 676
BstNI CCWGG 1 cut(s) 866
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 4 cut(s) 85, 86, 864, 905
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 2 cut(s) 569, 748
BstV1I GCAGC 2 cut(s) 663, 897
BstV2I GAAGAC 1 cut(s) 190
BstX2I RGATCY 3 cut(s) 53, 319, 772
BstYI RGATCY 3 cut(s) 53, 319, 772
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 2 cut(s) 340, 517
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 6 cut(s) 165, 452, 653, 843, 891, 922
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
CciI TCATGA 1 cut(s) 637
Cfr13I GGNCC 1 cut(s) 754
Cfr9I CCCGGG 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 2 cut(s) 121, 416
Csp6I GTAC 1 cut(s) 83
CviAII CATG 8 cut(s) 77, 237, 364, 638, 701, 714, 853, 932
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 6 cut(s) 170, 411, 461, 519, 588, 671
DpnI GATC 6 cut(s) 55, 321, 695, 774, 846, 938
DpnII GATC 6 cut(s) 53, 319, 693, 772, 844, 936
Eam1104I CTCTTC 2 cut(s) 27, 258
EarI CTCTTC 2 cut(s) 27, 258
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 297
Eco147I AGGCCT 1 cut(s) 517
Eco24I GRGCYC 2 cut(s) 270, 607
Eco47I GGWCC 1 cut(s) 754
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 3 cut(s) 184, 213, 282
Eco88I CYCGRG 3 cut(s) 86, 532, 562
EcoICRI GAGCTC 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 864
EcoT14I CCWWGG 1 cut(s) 297
EcoT22I ATGCAT 2 cut(s) 290, 454
EcoT38I GRGCYC 2 cut(s) 270, 607
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 8 cut(s) 80, 240, 367, 641, 704, 717, 856, 935
FatI CATG 8 cut(s) 76, 236, 363, 637, 700, 713, 852, 931
Fnu4HI GCNGC 2 cut(s) 677, 911
FokI GGATG 6 cut(s) 152, 439, 640, 850, 878, 929
FriOI GRGCYC 2 cut(s) 270, 607
Fsp4HI GCNGC 2 cut(s) 677, 911
FspBI CTAG 1 cut(s) 42
GluI GCNGC 2 cut(s) 677, 911
HaeIII GGCC 2 cut(s) 340, 517
HapII CCGG 2 cut(s) 87, 907
HgaI GACGC 2 cut(s) 121, 416
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 8 cut(s) 80, 240, 367, 641, 704, 717, 856, 935
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 5 cut(s) 215, 293, 487, 648, 808
HpaII CCGG 2 cut(s) 87, 907
Hpy166II GTNNAC 2 cut(s) 83, 559
Hpy188I TCNGA 3 cut(s) 403, 589, 834
Hpy188III TCNNGA 5 cut(s) 230, 282, 351, 638, 949
Hpy8I GTNNAC 2 cut(s) 83, 559
HpyAV CCTTC 2 cut(s) 44, 946
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 8 cut(s) 70, 179, 288, 317, 452, 704, 717, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 676
HpyF3I CTNAG 6 cut(s) 170, 411, 461, 519, 588, 671
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 8 cut(s) 80, 240, 367, 641, 704, 717, 856, 935
Kzo9I GATC 6 cut(s) 53, 319, 693, 772, 844, 936
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 2 cut(s) 92, 273
Lsp1109I GCAGC 2 cut(s) 663, 897
LweI GCATC 6 cut(s) 275, 297, 439, 461, 662, 828
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 112, 627
MalI GATC 6 cut(s) 55, 321, 695, 774, 846, 938
MboI GATC 6 cut(s) 53, 319, 693, 772, 844, 936
MboII GAAGA 8 cut(s) 44, 190, 254, 275, 379, 458, 626, 767
MfeI CAATTG 1 cut(s) 205
MflI RGATCY 3 cut(s) 53, 319, 772
MhlI GDGCHC 5 cut(s) 270, 569, 607, 748, 768
MluCI AATT 7 cut(s) 119, 180, 205, 500, 550, 724, 964
MlyI GAGTC 3 cut(s) 209, 657, 802
MmeI TCCRAC 1 cut(s) 732
Mph1103I ATGCAT 2 cut(s) 290, 454
MroXI GAANNNNTTC 2 cut(s) 246, 397
MseI TTAA 2 cut(s) 540, 967
MspI CCGG 2 cut(s) 87, 907
MspR9I CCNGG 4 cut(s) 87, 88, 866, 907
MunI CAATTG 1 cut(s) 205
Mva1269I GAATGC 1 cut(s) 828
MvaI CCWGG 1 cut(s) 866
MwoI GCNNNNNNNGC 2 cut(s) 308, 676
NciI CCSGG 3 cut(s) 87, 88, 907
NdeII GATC 6 cut(s) 53, 319, 693, 772, 844, 936
NlaIII CATG 8 cut(s) 80, 240, 367, 641, 704, 717, 856, 935
NlaIV GGNNCC 1 cut(s) 745
NsiI ATGCAT 2 cut(s) 290, 454
NspI RCATGY 1 cut(s) 240
PaeR7I CTCGAG 1 cut(s) 532
PagI TCATGA 1 cut(s) 637
PceI AGGCCT 1 cut(s) 517
PciI ACATGT 1 cut(s) 236
PciSI GCTCTTC 1 cut(s) 258
PctI GAATGC 1 cut(s) 828
PdmI GAANNNNTTC 2 cut(s) 246, 397
PfeI GAWTC 2 cut(s) 293, 487
PkrI GCNGC 2 cut(s) 678, 912
PleI GAGTC 3 cut(s) 209, 656, 802
PpsI GAGTC 3 cut(s) 209, 656, 802
PscI ACATGT 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 864
PspGI CCWGG 1 cut(s) 864
PspN4I GGNNCC 1 cut(s) 745
PspPI GGNCC 1 cut(s) 754
PspXI VCTCGAGB 1 cut(s) 532
PsuI RGATCY 3 cut(s) 53, 319, 772
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
SacI GAGCTC 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 540, 967
SatI GCNGC 2 cut(s) 677, 911
Sau3AI GATC 6 cut(s) 53, 319, 693, 772, 844, 936
Sau96I GGNCC 1 cut(s) 754
SchI GAGTC 3 cut(s) 209, 657, 802
ScrFI CCNGG 4 cut(s) 87, 88, 866, 907
SduI GDGCHC 5 cut(s) 270, 569, 607, 748, 768
SfaNI GCATC 6 cut(s) 275, 297, 439, 461, 662, 828
SfcI CTRYAG 1 cut(s) 342
Sfr274I CTCGAG 1 cut(s) 532
SinI GGWCC 1 cut(s) 754
SlaI CTCGAG 1 cut(s) 532
SmaI CCCGGG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
Sse9I AATT 7 cut(s) 119, 180, 205, 500, 550, 724, 964
SseBI AGGCCT 1 cut(s) 517
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 270
StuI AGGCCT 1 cut(s) 517
StyD4I CCNGG 4 cut(s) 85, 86, 864, 905
StyI CCWWGG 1 cut(s) 297
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 30, 291, 533, 843
TasI AATT 7 cut(s) 119, 180, 205, 500, 550, 724, 964
TfiI GAWTC 2 cut(s) 293, 487
Tru1I TTAA 2 cut(s) 540, 967
Tru9I TTAA 2 cut(s) 540, 967
TscAI CASTG 1 cut(s) 154
TseI GCWGC 2 cut(s) 676, 910
TspDTI ATGAA 4 cut(s) 65, 380, 689, 841
TspGWI ACGGA 3 cut(s) 405, 699, 877
TspMI CCCGGG 1 cut(s) 86
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 754
XapI RAATTY 2 cut(s) 119, 724
XceI RCATGY 1 cut(s) 240
XcmI CCANNNNNNNNNTGG 1 cut(s) 381
XhoI CTCGAG 1 cut(s) 532
XmaI CCCGGG 1 cut(s) 86
XmnI GAANNNNTTC 2 cut(s) 246, 397
XspI CTAG 1 cut(s) 42
Zsp2I ATGCAT 2 cut(s) 290, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.