Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0002522

Physical Location & Seq

Genomic Coordinates Forward (+)

12953836 .. 12957791

Gene Structure 3956 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027585

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGGCGTGCCTGAAGCTGGGATCGAAATCTGAGGTCTTTCATCTTGATGGCCAAACTTGGCTTTGCTCAACTGGACTGTCAAGTGATGTCATTGTTGAAGTTGAGGAGACGTCCTTCCATCTCCACAAGTTTCCATTGCTATCAAGAAGTGGATTATTACAAGACATTATTGCAGAATTTCCTAGTGAGGATGAGTACAAATGTAGTTTGCAACTGCATGACATTCCTGGTGGAGCCAAAGCCTTCTTGCTTGTAGCCAAATTCTGTTATGGTGTAAGATTTGAACTCAATGCCCTGAATGTAGTTATTGTGAGGTGTGCAGCTGAGTATCTTCGCATGAATGAAGACTATGGAGAAGGAAATCTCATCAGGCAAGCAGAGGATTTTCTAAATGATATACTTGGCAATTGGACTGACTCAATTAAAGTACTTGAAACCTGCGAAGAAGTCTTGCCTCATGCAGAAGAGCTTCATATTGTTTCAAGATGCATCAATTCATTGGCAATGACAGCTAGTGCTGATCCGAGTTTATTCAGTTGGCCTATGTCGGGTAAAGGTGCAGTGAAGAGCCCAGAAGGCGTGGCATTCTGGAATGGAATATGCATGACTGCCAAACCACATCCAGTTAATGAAGATTGGTGGTTTGAGGAAGTCTCCTTCCTGAAATTGCCTTTTTATAAAAGGCTGATTCGGGCTGTTGAATCAGGTGGTATGAATCTAGAGAAGGTTGCTGGTTCAATTATGCACTATGCAAAGAGGCATCTTTCCTTGTTGGGCAGGCAATCTAGTTTCCTAAATGGAAACCGTGCTGCCCCAGCATCAACTGTTTCTGCCCCATCTGATGTAGATCAAAGGAATCTTCTTGAGGAGATAGTGGAATTACTACCTGATCAGAAGGGCGTTACACCAACCAACTTCCTGCTTAGGCTTCTGCGGACATCTATGATTTTGCATGCTAGCCCATCATGTCGAGAGAATTTGGAAAAGCAAGTTGCAGCCCAGTTGGAACAAGCAGCTCTTGAAGATCTCCTGATACCAAACATGGGTTACACAGTGGAGACCCTCTATGATATTGATTGTGTTCAGCGGATTCTAGATCACTTCATGCTAATGGATCGCGATTCAATTGATTATTCTTCTAACTGTATAGTTGATGAAGGCCAGTTGAGTGATGGGTCTCATGCACTGACTCGAATGACCATGGTGGCTCATCTTGTGGATGGCTATCTTGCTGAGGTGGCACCTGATGTTAACTTGAAGTTACAGAAGTTTCAGTCACTGGCTGCTCATATCCCTGAATATGCAAGACCATTAGACGATGGACTTTACCGTGCTATCGACATATACCTCAAGGCCCATCCCTGGCTAACAGACTCTGAGAAGGAGCAAATCTGCAGGCTCATGAACTGCCAGAAGCTCTCATTGGAAGCCAGCACACATGCAGCCCAGAATGAGAGGTTACCACTCCGCATCATTGTCCAAGTTCTATTCTTTGAACAGCTTCGGCTACGGACTTCAGTTGCTGGTTGGTTCTTTGTATCTGACAACCTTGAGAACTCGCAGAATCTGAATGGAAATCTCGCGCTTGTTAGAAATGACGCACCTGCTCAAGCTGGGACCAGACGACTGGATCGTGTAGTAGCAGTCGATGACATGAAGGAGCGGGTTTCTGAGCTTGAGAAGGAATGCTTGAGCATGAAACAAGAGATTGAGAAGCTGGTGAAGACAAAAGGGAGTTGGAATTCCTTCTTGAAAAAGTTTGGTTTGAGGCTCAAGTCAAAGCCTGATGCTAAGAAACCATCCAAGCCTAGCTGCAATGGTTCCGAAGAATTACCAACATCAATAGGGCCACTTATGAATGGAAAAGAAAATTACGAAAATAAAGAATTAAGAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0016020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

633

Amino Acids

70.97

Weight (kDa)

5.42

Isoelectric Point (pI)

40.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	26 - 115	2.7e-06	BTB/POZ domain
NPH3	PF03000	212 - 478	2.7e-96	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03250 AT5G03250
fragaria_vesca	FvH4_6g16010 FvH4_7g19310 FvH4_7g19310 FvH4_7g19310
malus_domestica	MD01G1107700.v1.1 MD04G1096800.v1.1 MD07G1173300.v1.1
prunus_persica	Prupe.2G212500_v2.0.a1 Prupe.6G236100_v2.0.a1
pyrus_communis	pycom01g13460 pycom04g09190 pycom07g16800 pycom12g11690
rosa_chinensis	RchiOBHm_Chr1g0363191 RchiOBHm_Chr3g0469731
rosa_laevigata	RLG00000024326 RLG00000027585
rosa_multiflora	Rmu_sc0002925.1_g000004 Rmu_sc0010644.1_g000001 Rmu_sc0022819.1_g000002
rosa_roxburghii	Rroxscaffold_4G00293390 Rroxscaffold_6G00411520
rosa_rugosa	Rorug01G0305300 Rorug03G0103400 Rorug03G0103500 Rorug03G0103600 Rorug03G0103700 Rorug03G0103800 Rorug03G0103800
rosa_samantha	Rh1AG314100 Rh1BG277600 Rh1CG294000 Rh1DG308300 Rh3AG153200 Rh3BG177200 Rh3CG168100 Rh3DG198400
rosa_wichuraiana	Rw1G027830 Rw3G014440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	678
AarI	CACCTGC	1 cut(s)	1612
AatII	GACGTC	1 cut(s)	113
Acc36I	ACCTGC	2 cut(s)	446, 1612
AccB1I	GGYRCC	1 cut(s)	1240
AccBSI	CCGCTC	1 cut(s)	1663
AccII	CGCG	2 cut(s)	1119, 1583
AciI	CCGC	4 cut(s)	934, 1087, 1468, 1663
AclWI	GGATC	4 cut(s)	28, 515, 1122, 1638
AcoI	YGGCCR	1 cut(s)	49
AcsI	RAATTY	4 cut(s)	176, 260, 976, 1741
AcuI	CTGAAG	2 cut(s)	32, 1500
AcyI	GRCGYC	1 cut(s)	110
AfaI	GTAC	2 cut(s)	197, 429
AfiI	CCNNNNNNNGG	4 cut(s)	16, 548, 1043, 1362
AjnI	CCWGG	2 cut(s)	226, 1361
AjuI	GAANNNNNNNTTGG	2 cut(s)	1406, 1438
Alw26I	GTCTC	4 cut(s)	101, 658, 1052, 1182
AlwI	GGATC	4 cut(s)	28, 515, 1122, 1638
AlwNI	CAGNNNCTG	4 cut(s)	1279, 1376, 1523, 1567
AoxI	GGCC	5 cut(s)	49, 539, 1159, 1353, 1847
ApeKI	GCWGC	7 cut(s)	320, 809, 995, 1013, 1283, 1442, 1812
ApoI	RAATTY	4 cut(s)	176, 260, 976, 1741
Asp700I	GAANNNNTTC	3 cut(s)	468, 1500, 1745
AspLEI	GCGC	1 cut(s)	1585
AspS9I	GGNCC	3 cut(s)	1354, 1617, 1847
AsuHPI	GGTGA	1 cut(s)	1732
AsuNHI	GCTAGC	1 cut(s)	956
AvaII	GGWCC	1 cut(s)	1617
BalI	TGGCCA	1 cut(s)	51
BanI	GGYRCC	1 cut(s)	1240
BanII	GRGCYC	1 cut(s)	572
BbsI	GAAGAC	2 cut(s)	351, 1730
BbvCI	CCTCAGC	1 cut(s)	1233
BbvI	GCAGC	7 cut(s)	332, 796, 1007, 1025, 1270, 1454, 1799
BccI	CCATC	9 cut(s)	41, 126, 844, 970, 1166, 1214, 1313, 1365, 1807
BciT130I	CCWGG	2 cut(s)	228, 1363
BclI	TGATCA	1 cut(s)	889
BcoDI	GTCTC	4 cut(s)	101, 658, 1052, 1182
BfaI	CTAG	7 cut(s)	183, 513, 719, 786, 957, 1094, 1809
BfmI	CTRYAG	1 cut(s)	1393
BfuAI	ACCTGC	2 cut(s)	446, 1612
BglI	GCCNNNNNGGC	1 cut(s)	576
BglII	AGATCT	1 cut(s)	1024
BisI	GCNGC	7 cut(s)	321, 810, 996, 1014, 1284, 1443, 1813
BlsI	GCNGC	7 cut(s)	322, 811, 997, 1015, 1285, 1444, 1814
BmcAI	AGTACT	1 cut(s)	429
Bme1390I	CCNGG	2 cut(s)	228, 1363
Bme18I	GGWCC	1 cut(s)	1617
BmgT120I	GGNCC	3 cut(s)	1354, 1617, 1847
BmiI	GGNNCC	4 cut(s)	235, 1242, 1618, 1822
BmrFI	CCNGG	2 cut(s)	228, 1363
BmrI	ACTGGG	1 cut(s)	994
BmsI	GCATC	6 cut(s)	476, 498, 769, 827, 1479, 1777
BmtI	GCTAGC	1 cut(s)	960
BmuI	ACTGGG	1 cut(s)	994
BpiI	GAAGAC	2 cut(s)	351, 1730
BplI	GAGNNNNNCTC	2 cut(s)	638, 670
Bpu10I	CCTNAGC	2 cut(s)	923, 1233
BpuEI	CTTGAG	7 cut(s)	884, 1334, 1571, 1593, 1697, 1711, 1757
BsaBI	GATNNNNATC	3 cut(s)	25, 846, 1224
BsaHI	GRCGYC	1 cut(s)	110
BsaI	GGTCTC	2 cut(s)	1052, 1182
BsaJI	CCNNGG	2 cut(s)	1200, 1361
Bsc4I	CCNNNNNNNGG	4 cut(s)	16, 548, 1043, 1362
Bse1I	ACTGG	6 cut(s)	76, 623, 1000, 1162, 1284, 1632
Bse3DI	GCAATG	3 cut(s)	134, 510, 1822
Bse8I	GATNNNNATC	3 cut(s)	25, 846, 1224
BseBI	CCWGG	2 cut(s)	228, 1363
BseDI	CCNNGG	2 cut(s)	1200, 1361
BseGI	GGATG	5 cut(s)	196, 619, 1225, 1357, 1799
BseJI	GATNNNNATC	3 cut(s)	25, 846, 1224
BseLI	CCNNNNNNNGG	4 cut(s)	16, 548, 1043, 1362
BseMI	GCAATG	3 cut(s)	134, 510, 1822
BseMII	CTCAG	5 cut(s)	21, 315, 1224, 1368, 1662
BseNI	ACTGG	6 cut(s)	76, 623, 1000, 1162, 1284, 1632
BseRI	GAGGAG	2 cut(s)	119, 881
BseXI	GCAGC	7 cut(s)	332, 796, 1007, 1025, 1270, 1454, 1799
BseYI	CCCAGC	3 cut(s)	16, 814, 1613
BsgI	GTGCAG	2 cut(s)	339, 579
Bsh1236I	CGCG	2 cut(s)	1119, 1583
BshFI	GGCC	5 cut(s)	51, 541, 1161, 1355, 1849
BshNI	GGYRCC	1 cut(s)	1240
BslFI	GGGAC	1 cut(s)	1630
BslI	CCNNNNNNNGG	4 cut(s)	16, 548, 1043, 1362
BsmAI	GTCTC	4 cut(s)	101, 658, 1052, 1182
BsmBI	CGTCTC	1 cut(s)	101
BsmFI	GGGAC	1 cut(s)	1630
BsmI	GAATGC	2 cut(s)	584, 1691
BsnI	GGCC	5 cut(s)	51, 541, 1161, 1355, 1849
Bso31I	GGTCTC	2 cut(s)	1052, 1182
Bsp1286I	GDGCHC	1 cut(s)	572
Bsp143I	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
Bsp19I	CCATGG	1 cut(s)	1200
Bsp68I	TCGCGA	1 cut(s)	1119
BspACI	CCGC	4 cut(s)	934, 1087, 1468, 1663
BspANI	GGCC	5 cut(s)	51, 541, 1161, 1355, 1849
BspCNI	CTCAG	5 cut(s)	22, 316, 1225, 1369, 1663
BspFNI	CGCG	2 cut(s)	1119, 1583
BspHI	TCATGA	1 cut(s)	1401
BspLI	GGNNCC	4 cut(s)	235, 1242, 1618, 1822
BspMAI	CTGCAG	1 cut(s)	1397
BspMI	ACCTGC	2 cut(s)	446, 1612
BspOI	GCTAGC	1 cut(s)	960
BspPI	GGATC	4 cut(s)	28, 515, 1122, 1638
BspQI	GCTCTTC	2 cut(s)	459, 560
BspT107I	GGYRCC	1 cut(s)	1240
BspTNI	GGTCTC	2 cut(s)	1052, 1182
BsrBI	CCGCTC	1 cut(s)	1663
BsrDI	GCAATG	3 cut(s)	134, 510, 1822
BsrI	ACTGG	6 cut(s)	76, 623, 1000, 1162, 1284, 1632
BssECI	CCNNGG	2 cut(s)	1200, 1361
BssMI	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
BssNI	GRCGYC	1 cut(s)	110
BssT1I	CCWWGG	1 cut(s)	1200
Bst2UI	CCWGG	2 cut(s)	228, 1363
Bst4CI	ACNGT	6 cut(s)	78, 806, 826, 1054, 1145, 1331
Bst6I	CTCTTC	2 cut(s)	459, 560
BstACI	GRCGYC	1 cut(s)	110
BstC8I	GCNNGC	7 cut(s)	7, 375, 779, 954, 958, 1397, 1432
BstDEI	CTNAG	7 cut(s)	30, 324, 923, 1233, 1377, 1671, 1791
BstDSI	CCRYGG	1 cut(s)	1200
BstEII	GGTNACC	1 cut(s)	1458
BstF5I	GGATG	5 cut(s)	196, 619, 1225, 1357, 1799
BstFNI	CGCG	2 cut(s)	1119, 1583
BstHHI	GCGC	1 cut(s)	1585
BstKTI	GATC	8 cut(s)	23, 523, 850, 892, 1027, 1099, 1117, 1633
BstMAI	GTCTC	4 cut(s)	101, 658, 1052, 1182
BstMBI	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
BstMWI	GCNNNNNNNGC	4 cut(s)	509, 576, 815, 1238
BstNI	CCWGG	2 cut(s)	228, 1363
BstNSI	RCATGY	2 cut(s)	956, 1442
BstPI	GGTNACC	1 cut(s)	1458
BstSCI	CCNGG	2 cut(s)	226, 1361
BstSFI	CTRYAG	1 cut(s)	1393
BstUI	CGCG	2 cut(s)	1119, 1583
BstV1I	GCAGC	7 cut(s)	332, 796, 1007, 1025, 1270, 1454, 1799
BstV2I	GAAGAC	2 cut(s)	351, 1730
BstX2I	RGATCY	1 cut(s)	1024
BstXI	CCANNNNNNTGG	1 cut(s)	1627
BstYI	RGATCY	1 cut(s)	1024
BsuRI	GGCC	5 cut(s)	51, 541, 1161, 1355, 1849
BtgI	CCRYGG	1 cut(s)	1200
BtsCI	GGATG	5 cut(s)	196, 619, 1225, 1357, 1799
BtsI	GCAGTG	1 cut(s)	567
BtsIMutI	CAGTG	4 cut(s)	567, 1059, 1184, 1277
BtuMI	TCGCGA	1 cut(s)	1119
BveI	ACCTGC	2 cut(s)	446, 1612
Cac8I	GCNNGC	7 cut(s)	7, 375, 779, 954, 958, 1397, 1432
CaiI	CAGNNNCTG	4 cut(s)	1279, 1376, 1523, 1567
CciI	TCATGA	1 cut(s)	1401
CfoI	GCGC	1 cut(s)	1585
Cfr13I	GGNCC	3 cut(s)	1354, 1617, 1847
CseI	GACGC	1 cut(s)	1607
Csp6I	GTAC	2 cut(s)	196, 428
CviQI	GTAC	2 cut(s)	196, 428
DdeI	CTNAG	7 cut(s)	30, 324, 923, 1233, 1377, 1671, 1791
DpnI	GATC	8 cut(s)	22, 522, 849, 891, 1026, 1098, 1116, 1632
DpnII	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
EaeI	YGGCCR	1 cut(s)	49
Eam1104I	CTCTTC	2 cut(s)	459, 560
EarI	CTCTTC	2 cut(s)	459, 560
Eco130I	CCWWGG	1 cut(s)	1200
Eco24I	GRGCYC	1 cut(s)	572
Eco31I	GGTCTC	2 cut(s)	1052, 1182
Eco47I	GGWCC	1 cut(s)	1617
Eco57I	CTGAAG	2 cut(s)	32, 1500
Eco91I	GGTNACC	1 cut(s)	1458
EcoO65I	GGTNACC	1 cut(s)	1458
EcoRI	GAATTC	1 cut(s)	1741
EcoRII	CCWGG	2 cut(s)	226, 1361
EcoT14I	CCWWGG	1 cut(s)	1200
EcoT22I	ATGCAT	2 cut(s)	491, 605
EcoT38I	GRGCYC	1 cut(s)	572
ErhI	CCWWGG	1 cut(s)	1200
Esp3I	CGTCTC	1 cut(s)	101
FalI	AAGNNNNNCTT	4 cut(s)	1002, 1034, 1673, 1705
FaqI	GGGAC	1 cut(s)	1630
FauI	CCCGC	1 cut(s)	1656
FbaI	TGATCA	1 cut(s)	889
Fnu4HI	GCNGC	7 cut(s)	321, 810, 996, 1014, 1284, 1443, 1813
FokI	GGATG	5 cut(s)	203, 606, 1232, 1344, 1786
FriOI	GRGCYC	1 cut(s)	572
Fsp4HI	GCNGC	7 cut(s)	321, 810, 996, 1014, 1284, 1443, 1813
FspBI	CTAG	7 cut(s)	183, 513, 719, 786, 957, 1094, 1809
GlaI	GCGC	1 cut(s)	1584
GluI	GCNGC	7 cut(s)	321, 810, 996, 1014, 1284, 1443, 1813
GsaI	CCCAGC	3 cut(s)	20, 818, 1617
HaeIII	GGCC	5 cut(s)	51, 541, 1161, 1355, 1849
HgaI	GACGC	1 cut(s)	1607
HhaI	GCGC	1 cut(s)	1585
Hin1I	GRCGYC	1 cut(s)	110
Hin6I	GCGC	1 cut(s)	1583
HinP1I	GCGC	1 cut(s)	1583
HincII	GTYRAC	1 cut(s)	1252
HindII	GTYRAC	1 cut(s)	1252
HpaI	GTTAAC	1 cut(s)	1252
HphI	GGTGA	1 cut(s)	1732
Hpy166II	GTNNAC	1 cut(s)	1252
Hpy188I	TCNGA	9 cut(s)	31, 525, 841, 894, 1378, 1543, 1569, 1672, 1825
Hpy8I	GTNNAC	1 cut(s)	1252
HpyCH4III	ACNGT	6 cut(s)	78, 806, 826, 1054, 1145, 1331
HpyCH4IV	ACGT	1 cut(s)	110
HpyF10VI	GCNNNNNNNGC	4 cut(s)	509, 576, 815, 1238
HpyF3I	CTNAG	7 cut(s)	30, 324, 923, 1233, 1377, 1671, 1791
HpySE526I	ACGT	1 cut(s)	110
Hsp92I	GRCGYC	1 cut(s)	110
HspAI	GCGC	1 cut(s)	1583
Ksp22I	TGATCA	1 cut(s)	889
KspAI	GTTAAC	1 cut(s)	1252
Kzo9I	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
LguI	GCTCTTC	2 cut(s)	459, 560
LmnI	GCTCC	3 cut(s)	233, 1384, 1660
Lsp1109I	GCAGC	7 cut(s)	332, 796, 1007, 1025, 1270, 1454, 1799
LweI	GCATC	6 cut(s)	476, 498, 769, 827, 1479, 1777
MaeI	CTAG	7 cut(s)	183, 513, 719, 786, 957, 1094, 1809
MaeII	ACGT	1 cut(s)	110
MaeIII	GTNAC	5 cut(s)	901, 1046, 1260, 1275, 1458
MalI	GATC	8 cut(s)	22, 522, 849, 891, 1026, 1098, 1116, 1632
MbiI	CCGCTC	1 cut(s)	1663
MboI	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
MfeI	CAATTG	2 cut(s)	406, 1125
MflI	RGATCY	1 cut(s)	1024
MhlI	GDGCHC	1 cut(s)	572
MlsI	TGGCCA	1 cut(s)	51
MluNI	TGGCCA	1 cut(s)	51
MlyI	GAGTC	3 cut(s)	410, 1183, 1367
MmeI	TCCRAC	2 cut(s)	984, 1718
Mox20I	TGGCCA	1 cut(s)	51
Mph1103I	ATGCAT	2 cut(s)	491, 605
MroXI	GAANNNNTTC	3 cut(s)	468, 1500, 1745
MscI	TGGCCA	1 cut(s)	51
MseI	TTAA	4 cut(s)	423, 627, 1251, 1889
MslI	CAYNNNNRTG	2 cut(s)	45, 1109
Msp20I	TGGCCA	1 cut(s)	51
MspA1I	CMGCKG	2 cut(s)	323, 1087
MspR9I	CCNGG	2 cut(s)	228, 1363
MunI	CAATTG	2 cut(s)	406, 1125
Mva1269I	GAATGC	2 cut(s)	584, 1691
MvaI	CCWGG	2 cut(s)	228, 1363
MvnI	CGCG	2 cut(s)	1119, 1583
MwoI	GCNNNNNNNGC	4 cut(s)	509, 576, 815, 1238
NcoI	CCATGG	1 cut(s)	1200
NdeII	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
NheI	GCTAGC	1 cut(s)	956
NlaIV	GGNNCC	4 cut(s)	235, 1242, 1618, 1822
NmuCI	GTSAC	1 cut(s)	1275
NruI	TCGCGA	1 cut(s)	1119
NsiI	ATGCAT	2 cut(s)	491, 605
NspI	RCATGY	2 cut(s)	956, 1442
PaeI	GCATGC	1 cut(s)	956
PagI	TCATGA	1 cut(s)	1401
PaqCI	CACCTGC	1 cut(s)	1612
PciSI	GCTCTTC	2 cut(s)	459, 560
PcsI	WCGNNNNNNNCGW	1 cut(s)	1630
PctI	GAATGC	2 cut(s)	584, 1691
PdmI	GAANNNNTTC	3 cut(s)	468, 1500, 1745
PfeI	GAWTC	7 cut(s)	688, 701, 715, 856, 1090, 1121, 1564
PkrI	GCNGC	7 cut(s)	322, 811, 997, 1015, 1285, 1444, 1814
PleI	GAGTC	3 cut(s)	410, 1183, 1367
PpsI	GAGTC	3 cut(s)	410, 1183, 1367
PsiI	TTATAA	1 cut(s)	678
Psp6I	CCWGG	2 cut(s)	226, 1361
PspEI	GGTNACC	1 cut(s)	1458
PspFI	CCCAGC	3 cut(s)	16, 814, 1613
PspGI	CCWGG	2 cut(s)	226, 1361
PspN4I	GGNNCC	4 cut(s)	235, 1242, 1618, 1822
PspPI	GGNCC	3 cut(s)	1354, 1617, 1847
PstI	CTGCAG	1 cut(s)	1397
PstNI	CAGNNNCTG	4 cut(s)	1279, 1376, 1523, 1567
PsuI	RGATCY	1 cut(s)	1024
PvuII	CAGCTG	1 cut(s)	323
RruI	TCGCGA	1 cut(s)	1119
RsaI	GTAC	2 cut(s)	197, 429
RsaNI	GTAC	2 cut(s)	196, 428
RseI	CAYNNNNRTG	2 cut(s)	45, 1109
SapI	GCTCTTC	2 cut(s)	459, 560
SaqAI	TTAA	4 cut(s)	423, 627, 1251, 1889
SatI	GCNGC	7 cut(s)	321, 810, 996, 1014, 1284, 1443, 1813
Sau3AI	GATC	8 cut(s)	20, 520, 847, 889, 1024, 1096, 1114, 1630
Sau96I	GGNCC	3 cut(s)	1354, 1617, 1847
ScaI	AGTACT	1 cut(s)	429
SchI	GAGTC	3 cut(s)	410, 1183, 1367
ScrFI	CCNGG	2 cut(s)	228, 1363
SduI	GDGCHC	1 cut(s)	572
SfaNI	GCATC	6 cut(s)	476, 498, 769, 827, 1479, 1777
SfcI	CTRYAG	1 cut(s)	1393
SinI	GGWCC	1 cut(s)	1617
SmiMI	CAYNNNNRTG	2 cut(s)	45, 1109
SmlI	CTYRAG	7 cut(s)	863, 1349, 1550, 1608, 1676, 1690, 1772
SmoI	CTYRAG	7 cut(s)	863, 1349, 1550, 1608, 1676, 1690, 1772
SphI	GCATGC	1 cut(s)	956
SsiI	CCGC	4 cut(s)	934, 1087, 1468, 1663
SspMI	CTAG	7 cut(s)	183, 513, 719, 786, 957, 1094, 1809
StyD4I	CCNGG	2 cut(s)	226, 1361
StyI	CCWWGG	1 cut(s)	1200
TaaI	ACNGT	6 cut(s)	78, 806, 826, 1054, 1145, 1331
TaiI	ACGT	1 cut(s)	113
TaqI	TCGA	5 cut(s)	23, 970, 1192, 1338, 1647
TatI	WGTACW	2 cut(s)	195, 427
TfiI	GAWTC	7 cut(s)	688, 701, 715, 856, 1090, 1121, 1564
Tru1I	TTAA	4 cut(s)	423, 627, 1251, 1889
Tru9I	TTAA	4 cut(s)	423, 627, 1251, 1889
TscAI	CASTG	4 cut(s)	567, 1059, 1191, 1284
TseFI	GTSAC	1 cut(s)	1275
TseI	GCWGC	7 cut(s)	320, 809, 995, 1013, 1283, 1442, 1812
Tsp45I	GTSAC	1 cut(s)	1275
TspGWI	ACGGA	1 cut(s)	1525
TspRI	CASTG	4 cut(s)	567, 1059, 1191, 1284
VpaK11BI	GGWCC	1 cut(s)	1617
XapI	RAATTY	4 cut(s)	176, 260, 976, 1741
XbaI	TCTAGA	2 cut(s)	718, 1093
XceI	RCATGY	2 cut(s)	956, 1442
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1169
XmnI	GAANNNNTTC	3 cut(s)	468, 1500, 1745
XspI	CTAG	7 cut(s)	183, 513, 719, 786, 957, 1094, 1809
ZraI	GACGTC	1 cut(s)	111
ZrmI	AGTACT	1 cut(s)	429
Zsp2I	ATGCAT	2 cut(s)	491, 605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.