Rh1CG294000

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002522
Physical Location & Seq
Reverse (-)
56661275 .. 56663213
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG294000.1

Sequence Viewer

Length: 1395 bp
ATGACAGCTAGTGCTGATCCGAGTTTATTCAGTTGGCCTATGTCGGGTAAAGGTGCAGTGAAGAGCCCAGAAGGCGTGGCATTCTGGAATGGAATAAGCACGACTGCCAAACCACATCCAGTTAATGAAGATTGGTGGTTTGAGGAAATCTCCTTCCTGAAATTGCCTTTTTATAAAAGGCTGATTCGGGCTGTTGAATCAGGTGGTATGAATCTAGAGAAGGTTGCTGGTTCAATTATGCACTATGCAAAGAAGCATCTTTCCTTGTTGGGCAGGCAATCTAGTTTCCTAAATGGAAACCGTGCTGCCCCAGCATCAACTGTTTCTGCCCCATCTGATGTAGATCAAAGGAATCTTCTTGAGGAGATAGTGGAATTACTACCTGATCAGAAGGGCGTTACACCAACCAACTTCCTGCTTAGGCTTCTGCGGACATCTATGATTTTGCATGCTAGCCCATCATGTCGAGAGAATTTGGAAAAGCGGGTTGGGGCCCAGTTGGAACAAGCAGCTCTTGAAGATCTCCTGATACCAAACATGGGTTACACAGTGGAGACCCTCTATGATATTGATTGTGTTCAGCGGATTCTTGATCACTTCATGCTAATGGATCGCGATTCAATTGATTATTCTTCTAACTGTATAGTTGATGAAGGCCAGTTGAGTGATGGGTCTCATGCACTGACTCGAATGACCATGGTGGCTCATCTTGTGGATGGCTATCTTGCTGAGGTGGCACCTGATGTTAACTTGAAGTTACAGAAGTTTCAGTCACTAGCTGCTCTTATCCCTGAATATGCAAGACCATTAGACGATGGACTTTACCGTGCTATCGACATATACCTCAAGGCCCATCCCTGGCTAACAGACTCTGAGAAGGAGCAAATCTGCAGGCTCATGAACTGCCAGAAGCTGTCATTGGAAGCCAGCACACATGCAGCCCAGAATGAGAGGTTACCACTCCGCGTCATTGTCCAAGTTCTTTTCTTTGAACAGCTTCGGCTACGGACTTCAGTTGCCGGTTGGTTCTTTGTATCTGACAACCTTGAGAACTCACAGAATCTGAGTGGAAATCTCGCGCTTGTTAGAAATGAAGCACCTGCTCAAGCTGGCACCAGACGACTGGATCGTGTTGTAGCTGTCGATGACATGAAGGAGCGGGTTTCTGAGCTTGAGAAGGAATGCTTGAGCATGAAACAAGAGATTGAGAAGCTGGTGAAGACAAAAGGGAGTTGGAATTCCTTGTTGAAAAAGTTTGGTTTGAGGCTCAAGTCAAAGCCTGATGCTAAGAAACCATCCAAGCCTAGCTGCAATGGTTCCGAAGAATTACCAACATCAATAGGGCCACTTATGGATGGAAAAGAAAATTACGAAAATAAAGAATTAAGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

464

Amino Acids

52.04

Weight (kDa)

5.95

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 44 - 310 6.3e-98 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AarI CACCTGC 1 cut(s) 1108
Acc36I ACCTGC 1 cut(s) 1108
AccB1I GGYRCC 2 cut(s) 736, 1112
AccBSI CCGCTC 1 cut(s) 1159
AccII CGCG 3 cut(s) 615, 966, 1079
AciI CCGC 5 cut(s) 430, 484, 583, 964, 1159
AclWI GGATC 3 cut(s) 11, 618, 1134
AcsI RAATTY 2 cut(s) 472, 1237
AcuI CTGAAG 1 cut(s) 996
AfiI CCNNNNNNNGG 3 cut(s) 44, 539, 858
AgsI TTSAA 7 cut(s) 197, 234, 518, 621, 754, 992, 1249
AjnI CCWGG 1 cut(s) 857
AjuI GAANNNNNNNTTGG 4 cut(s) 471, 503, 902, 934
Alw26I GTCTC 2 cut(s) 548, 678
AlwI GGATC 3 cut(s) 11, 618, 1134
AlwNI CAGNNNCTG 3 cut(s) 872, 913, 1063
AoxI GGCC 5 cut(s) 35, 492, 655, 849, 1343
ApaI GGGCCC 1 cut(s) 496
ApeKI GCWGC 5 cut(s) 305, 509, 779, 938, 1308
ApoI RAATTY 2 cut(s) 472, 1237
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 1 cut(s) 1081
AspS9I GGNCC 4 cut(s) 492, 493, 850, 1343
AsuHPI GGTGA 1 cut(s) 1228
AsuNHI GCTAGC 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 496
BanI GGYRCC 2 cut(s) 736, 1112
BanII GRGCYC 2 cut(s) 68, 496
BbsI GAAGAC 1 cut(s) 1226
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 5 cut(s) 292, 521, 766, 950, 1295
BccI CCATC 8 cut(s) 340, 466, 662, 710, 809, 861, 1303, 1349
BciT130I CCWGG 1 cut(s) 859
BclI TGATCA 2 cut(s) 385, 592
BcoDI GTCTC 2 cut(s) 548, 678
BfaI CTAG 6 cut(s) 9, 215, 282, 453, 776, 1305
BfmI CTRYAG 1 cut(s) 889
BfuAI ACCTGC 1 cut(s) 1108
BglI GCCNNNNNGGC 1 cut(s) 72
BglII AGATCT 1 cut(s) 520
BisI GCNGC 5 cut(s) 306, 510, 780, 939, 1309
BlsI GCNGC 5 cut(s) 307, 511, 781, 940, 1310
Bme1390I CCNGG 1 cut(s) 859
BmgT120I GGNCC 4 cut(s) 492, 493, 850, 1343
BmiI GGNNCC 5 cut(s) 493, 494, 738, 1114, 1318
BmrFI CCNGG 1 cut(s) 859
BmrI ACTGGG 1 cut(s) 490
BmsI GCATC 3 cut(s) 265, 323, 1273
BmtI GCTAGC 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 490
BpiI GAAGAC 1 cut(s) 1226
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu10I CCTNAGC 2 cut(s) 419, 729
BpuEI CTTGAG 7 cut(s) 380, 830, 1067, 1089, 1193, 1207, 1253
BsaBI GATNNNNATC 2 cut(s) 342, 720
BsaI GGTCTC 2 cut(s) 548, 678
BsaJI CCNNGG 2 cut(s) 696, 857
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 539, 858
Bse118I RCCGGY 1 cut(s) 1019
Bse1I ACTGG 4 cut(s) 119, 496, 658, 1128
Bse3DI GCAATG 1 cut(s) 1318
Bse8I GATNNNNATC 2 cut(s) 342, 720
BseBI CCWGG 1 cut(s) 859
BseDI CCNNGG 2 cut(s) 696, 857
BseGI GGATG 5 cut(s) 115, 721, 853, 1295, 1360
BseJI GATNNNNATC 2 cut(s) 342, 720
BseLI CCNNNNNNNGG 3 cut(s) 44, 539, 858
BseMI GCAATG 1 cut(s) 1318
BseMII CTCAG 4 cut(s) 720, 864, 1055, 1158
BseNI ACTGG 4 cut(s) 119, 496, 658, 1128
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 5 cut(s) 292, 521, 766, 950, 1295
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 1 cut(s) 75
Bsh1236I CGCG 3 cut(s) 615, 966, 1079
BshFI GGCC 5 cut(s) 37, 494, 657, 851, 1345
BshNI GGYRCC 2 cut(s) 736, 1112
BsiSI CCGG 1 cut(s) 1020
BslI CCNNNNNNNGG 3 cut(s) 44, 539, 858
BsmAI GTCTC 2 cut(s) 548, 678
BsmI GAATGC 2 cut(s) 80, 1187
BsnI GGCC 5 cut(s) 37, 494, 657, 851, 1345
Bso31I GGTCTC 2 cut(s) 548, 678
Bsp120I GGGCCC 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 68, 496
Bsp143I GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
Bsp19I CCATGG 1 cut(s) 696
Bsp68I TCGCGA 1 cut(s) 615
BspACI CCGC 5 cut(s) 430, 484, 583, 964, 1159
BspANI GGCC 5 cut(s) 37, 494, 657, 851, 1345
BspCNI CTCAG 4 cut(s) 721, 865, 1056, 1159
BspFNI CGCG 3 cut(s) 615, 966, 1079
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 5 cut(s) 493, 494, 738, 1114, 1318
BspMAI CTGCAG 1 cut(s) 893
BspMI ACCTGC 1 cut(s) 1108
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 3 cut(s) 11, 618, 1134
BspQI GCTCTTC 1 cut(s) 56
BspT107I GGYRCC 2 cut(s) 736, 1112
BspTNI GGTCTC 2 cut(s) 548, 678
BsrBI CCGCTC 1 cut(s) 1159
BsrDI GCAATG 1 cut(s) 1318
BsrFI RCCGGY 1 cut(s) 1019
BsrI ACTGG 4 cut(s) 119, 496, 658, 1128
BssAI RCCGGY 1 cut(s) 1019
BssECI CCNNGG 2 cut(s) 696, 857
BssMI GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 859
Bst4CI ACNGT 5 cut(s) 302, 322, 550, 641, 827
Bst6I CTCTTC 1 cut(s) 56
BstC8I GCNNGC 6 cut(s) 275, 450, 454, 893, 928, 1111
BstDEI CTNAG 6 cut(s) 419, 729, 873, 1064, 1167, 1287
BstDSI CCRYGG 1 cut(s) 696
BstEII GGTNACC 1 cut(s) 954
BstF5I GGATG 5 cut(s) 115, 721, 853, 1295, 1360
BstFNI CGCG 3 cut(s) 615, 966, 1079
BstHHI GCGC 1 cut(s) 1081
BstKTI GATC 7 cut(s) 19, 346, 388, 523, 595, 613, 1129
BstMAI GTCTC 2 cut(s) 548, 678
BstMBI GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 72, 311, 734
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 2 cut(s) 452, 938
BstPI GGTNACC 1 cut(s) 954
BstSCI CCNGG 1 cut(s) 857
BstSFI CTRYAG 1 cut(s) 889
BstSLI GKGCMC 1 cut(s) 496
BstUI CGCG 3 cut(s) 615, 966, 1079
BstV1I GCAGC 5 cut(s) 292, 521, 766, 950, 1295
BstV2I GAAGAC 1 cut(s) 1226
BstX2I RGATCY 1 cut(s) 520
BstXI CCANNNNNNTGG 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 5 cut(s) 37, 494, 657, 851, 1345
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 5 cut(s) 115, 721, 853, 1295, 1360
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 63, 555, 680
BtuMI TCGCGA 1 cut(s) 615
BveI ACCTGC 1 cut(s) 1108
Cac8I GCNNGC 6 cut(s) 275, 450, 454, 893, 928, 1111
CaiI CAGNNNCTG 3 cut(s) 872, 913, 1063
CciI TCATGA 1 cut(s) 897
CfoI GCGC 1 cut(s) 1081
Cfr10I RCCGGY 1 cut(s) 1019
Cfr13I GGNCC 4 cut(s) 492, 493, 850, 1343
CseI GACGC 1 cut(s) 955
DdeI CTNAG 6 cut(s) 419, 729, 873, 1064, 1167, 1287
DpnI GATC 7 cut(s) 18, 345, 387, 522, 594, 612, 1128
DpnII GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 2 cut(s) 68, 496
Eco31I GGTCTC 2 cut(s) 548, 678
Eco57I CTGAAG 1 cut(s) 996
Eco91I GGTNACC 1 cut(s) 954
EcoO109I RGGNCCY 1 cut(s) 492
EcoO65I GGTNACC 1 cut(s) 954
EcoRI GAATTC 1 cut(s) 1237
EcoRII CCWGG 1 cut(s) 857
EcoT14I CCWWGG 1 cut(s) 696
EcoT38I GRGCYC 2 cut(s) 68, 496
ErhI CCWWGG 1 cut(s) 696
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1169, 1201
FauI CCCGC 2 cut(s) 477, 1152
FbaI TGATCA 2 cut(s) 385, 592
Fnu4HI GCNGC 5 cut(s) 306, 510, 780, 939, 1309
FokI GGATG 5 cut(s) 102, 728, 840, 1282, 1367
FriOI GRGCYC 2 cut(s) 68, 496
Fsp4HI GCNGC 5 cut(s) 306, 510, 780, 939, 1309
FspBI CTAG 6 cut(s) 9, 215, 282, 453, 776, 1305
GlaI GCGC 1 cut(s) 1080
GluI GCNGC 5 cut(s) 306, 510, 780, 939, 1309
GsaI CCCAGC 1 cut(s) 314
HaeIII GGCC 5 cut(s) 37, 494, 657, 851, 1345
HapII CCGG 1 cut(s) 1020
HgaI GACGC 1 cut(s) 955
HhaI GCGC 1 cut(s) 1081
Hin6I GCGC 1 cut(s) 1079
HinP1I GCGC 1 cut(s) 1079
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HinfI GANTC 9 cut(s) 184, 197, 211, 352, 586, 617, 685, 869, 1060
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 1 cut(s) 1020
HphI GGTGA 1 cut(s) 1228
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 8 cut(s) 21, 337, 390, 874, 1039, 1065, 1168, 1321
Hpy8I GTNNAC 1 cut(s) 748
HpyAV CCTTC 8 cut(s) 65, 163, 214, 385, 647, 871, 1147, 1171
HpyCH4III ACNGT 5 cut(s) 302, 322, 550, 641, 827
HpyCH4V TGCA 9 cut(s) 56, 241, 248, 448, 680, 800, 891, 938, 1311
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 311, 734
HpyF3I CTNAG 6 cut(s) 419, 729, 873, 1064, 1167, 1287
HspAI GCGC 1 cut(s) 1079
Ksp22I TGATCA 2 cut(s) 385, 592
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 2 cut(s) 880, 1156
Lsp1109I GCAGC 5 cut(s) 292, 521, 766, 950, 1295
LweI GCATC 3 cut(s) 265, 323, 1273
MaeI CTAG 6 cut(s) 9, 215, 282, 453, 776, 1305
MaeIII GTNAC 5 cut(s) 397, 542, 756, 771, 954
MalI GATC 7 cut(s) 18, 345, 387, 522, 594, 612, 1128
MbiI CCGCTC 1 cut(s) 1159
MboI GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
MboII GAAGA 7 cut(s) 73, 140, 347, 530, 624, 1231, 1334
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 68, 496
MluCI AATT 9 cut(s) 161, 234, 374, 472, 621, 1237, 1325, 1366, 1382
MlyI GAGTC 2 cut(s) 679, 863
MmeI TCCRAC 2 cut(s) 480, 1214
MnlI CCTC 7 cut(s) 136, 355, 569, 724, 854, 945, 1257
MroXI GAANNNNTTC 1 cut(s) 996
MseI TTAA 3 cut(s) 123, 747, 1385
MslI CAYNNNNRTG 1 cut(s) 605
MspA1I CMGCKG 1 cut(s) 583
MspI CCGG 1 cut(s) 1020
MspR9I CCNGG 1 cut(s) 859
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 2 cut(s) 80, 1187
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 3 cut(s) 615, 966, 1079
MwoI GCNNNNNNNGC 3 cut(s) 72, 311, 734
NcoI CCATGG 1 cut(s) 696
NdeII GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
NheI GCTAGC 1 cut(s) 452
NlaIV GGNNCC 5 cut(s) 493, 494, 738, 1114, 1318
NmuCI GTSAC 1 cut(s) 771
NruI TCGCGA 1 cut(s) 615
NspI RCATGY 2 cut(s) 452, 938
PaeI GCATGC 1 cut(s) 452
PagI TCATGA 1 cut(s) 897
PaqCI CACCTGC 1 cut(s) 1108
PciSI GCTCTTC 1 cut(s) 56
PcsI WCGNNNNNNNCGW 1 cut(s) 1126
PctI GAATGC 2 cut(s) 80, 1187
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 7 cut(s) 184, 197, 211, 352, 586, 617, 1060
PkrI GCNGC 5 cut(s) 307, 511, 781, 940, 1310
PleI GAGTC 2 cut(s) 679, 863
PpsI GAGTC 2 cut(s) 679, 863
PsiI TTATAA 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 857
PspEI GGTNACC 1 cut(s) 954
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 1 cut(s) 857
PspN4I GGNNCC 5 cut(s) 493, 494, 738, 1114, 1318
PspOMI GGGCCC 1 cut(s) 492
PspPI GGNCC 4 cut(s) 492, 493, 850, 1343
PstI CTGCAG 1 cut(s) 893
PstNI CAGNNNCTG 3 cut(s) 872, 913, 1063
PsuI RGATCY 1 cut(s) 520
RruI TCGCGA 1 cut(s) 615
RseI CAYNNNNRTG 1 cut(s) 605
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 3 cut(s) 123, 747, 1385
SatI GCNGC 5 cut(s) 306, 510, 780, 939, 1309
Sau3AI GATC 7 cut(s) 16, 343, 385, 520, 592, 610, 1126
Sau96I GGNCC 4 cut(s) 492, 493, 850, 1343
SchI GAGTC 2 cut(s) 679, 863
ScrFI CCNGG 1 cut(s) 859
SduI GDGCHC 2 cut(s) 68, 496
SfaNI GCATC 3 cut(s) 265, 323, 1273
SfcI CTRYAG 1 cut(s) 889
SmiMI CAYNNNNRTG 1 cut(s) 605
SmlI CTYRAG 7 cut(s) 359, 845, 1046, 1104, 1172, 1186, 1268
SmoI CTYRAG 7 cut(s) 359, 845, 1046, 1104, 1172, 1186, 1268
SphI GCATGC 1 cut(s) 452
Sse9I AATT 9 cut(s) 161, 234, 374, 472, 621, 1237, 1325, 1366, 1382
SsiI CCGC 5 cut(s) 430, 484, 583, 964, 1159
SspMI CTAG 6 cut(s) 9, 215, 282, 453, 776, 1305
StyD4I CCNGG 1 cut(s) 857
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 5 cut(s) 302, 322, 550, 641, 827
TaqI TCGA 4 cut(s) 466, 688, 834, 1143
TasI AATT 9 cut(s) 161, 234, 374, 472, 621, 1237, 1325, 1366, 1382
TfiI GAWTC 7 cut(s) 184, 197, 211, 352, 586, 617, 1060
Tru1I TTAA 3 cut(s) 123, 747, 1385
Tru9I TTAA 3 cut(s) 123, 747, 1385
TscAI CASTG 3 cut(s) 63, 555, 687
TseFI GTSAC 1 cut(s) 771
TseI GCWGC 5 cut(s) 305, 509, 779, 938, 1308
Tsp45I GTSAC 1 cut(s) 771
TspDTI ATGAA 8 cut(s) 141, 224, 589, 666, 914, 1107, 1166, 1208
TspGWI ACGGA 1 cut(s) 1021
TspRI CASTG 3 cut(s) 63, 555, 687
XapI RAATTY 2 cut(s) 472, 1237
XbaI TCTAGA 1 cut(s) 214
XceI RCATGY 2 cut(s) 452, 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 6 cut(s) 9, 215, 282, 453, 776, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.