Rorug03G0103700

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002522
Physical Location & Seq
Forward (+)
8162650 .. 8164292
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0103700.1

Sequence Viewer

Length: 435 bp
ATGCAGAACAACAAGGCTATTTTGGAGGCTTTGTACAAGGCATTGGCCCAAGGGCAAACCGAGACGGTGGCAAAGGTTCTAGCAACTGACCTGGAATGGTGGTTCCATGGTCCTCCGAAGTGCCAGCACATGATGCGGGTGCTCACAGGGGATTCCAGCCACGTTGGCTTCAAGTTCGAGCCGAGAAGCGTAACGGAGATTGGCGACTACTGTGTGATTGCTGAGGGCTGGGAAGGAGCTCAGGCTTATTGGGTGCATGTGTGGACGATCAAGGATGGTTTGATCACTCAGTTCAGGGAGTATTTCAACACGTGGCTGACAGTGAGGGATCTGAAGCCGCTAGGGTGGAAGGAGAGTAAGAGCTTCACTGTGTGGCAGAGCATGCCAAGGGACCTGTTCCACCGTTCTTTGCCAAGCCTTCTCCTAGCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

144

Amino Acids

16.67

Weight (kDa)

6.96

Isoelectric Point (pI)

25.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 45 - 144 4.6e-33 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 136, 338
AclWI GGATC 1 cut(s) 336
AcuI CTGAAG 1 cut(s) 353
AcvI CACGTG 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 2 cut(s) 172, 307
AjnI CCWGG 1 cut(s) 90
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
AluBI AGCT 3 cut(s) 239, 363, 428
AluI AGCT 3 cut(s) 239, 363, 428
Alw21I GWGCWC 2 cut(s) 144, 241
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 336
AoxI GGCC 1 cut(s) 45
AspS9I GGNCC 3 cut(s) 46, 110, 391
AvaII GGWCC 2 cut(s) 110, 391
BanII GRGCYC 1 cut(s) 241
BbrPI CACGTG 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 144, 241
BbvCI CCTCAGC 1 cut(s) 222
BccI CCATC 1 cut(s) 269
BciT130I CCWGG 1 cut(s) 92
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 3 cut(s) 80, 341, 425
BglI GCCNNNNNGGC 1 cut(s) 165
BisI GCNGC 1 cut(s) 338
BlsI GCNGC 1 cut(s) 339
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 2 cut(s) 110, 391
BmgT120I GGNCC 3 cut(s) 46, 110, 391
BmiI GGNNCC 2 cut(s) 104, 392
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 1 cut(s) 123
Bpu10I CCTNAGC 2 cut(s) 222, 240
BsaAI YACGTR 1 cut(s) 312
BsaJI CCNNGG 3 cut(s) 49, 106, 386
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 3 cut(s) 49, 106, 386
BseGI GGATG 1 cut(s) 280
BseMII CTCAG 3 cut(s) 213, 254, 302
BseYI CCCAGC 1 cut(s) 228
BshFI GGCC 1 cut(s) 47
BsiHKAI GWGCWC 2 cut(s) 144, 241
BslFI GGGAC 1 cut(s) 404
BsmAI GTCTC 1 cut(s) 56
BsmBI CGTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 144, 241
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 3 cut(s) 267, 282, 328
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 2 cut(s) 136, 338
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 3 cut(s) 214, 253, 301
BspLI GGNNCC 2 cut(s) 104, 392
BspPI GGATC 1 cut(s) 336
BsrGI TGTACA 1 cut(s) 33
BssECI CCNNGG 3 cut(s) 49, 106, 386
BssMI GATC 3 cut(s) 267, 282, 328
BssT1I CCWWGG 3 cut(s) 49, 106, 386
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 5 cut(s) 67, 212, 322, 370, 404
BstAPI GCANNNNNTGC 2 cut(s) 133, 382
BstAUI TGTACA 1 cut(s) 33
BstBAI YACGTR 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 125, 383
BstDEI CTNAG 3 cut(s) 222, 240, 288
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 280
BstKTI GATC 3 cut(s) 270, 285, 331
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 3 cut(s) 267, 282, 328
BstMWI GCNNNNNNNGC 3 cut(s) 133, 165, 382
BstNI CCWGG 1 cut(s) 92
BstNSI RCATGY 2 cut(s) 260, 385
BstSCI CCNGG 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 327, 366
Cac8I GCNNGC 2 cut(s) 125, 383
Cfr13I GGNCC 3 cut(s) 46, 110, 391
Csp6I GTAC 1 cut(s) 34
CviAII CATG 4 cut(s) 107, 130, 257, 382
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 3 cut(s) 222, 240, 288
DpnI GATC 3 cut(s) 269, 284, 330
DpnII GATC 3 cut(s) 267, 282, 328
DraIII CACNNNGTG 1 cut(s) 372
Ecl136II GAGCTC 1 cut(s) 239
Eco130I CCWWGG 3 cut(s) 49, 106, 386
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 110, 391
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 353
Eco72I CACGTG 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 3 cut(s) 49, 106, 386
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 3 cut(s) 49, 106, 386
Esp3I CGTCTC 1 cut(s) 56
FaeI CATG 4 cut(s) 110, 133, 260, 385
FaiI YATR 4 cut(s) 108, 131, 258, 383
FaqI GGGAC 1 cut(s) 404
FatI CATG 4 cut(s) 106, 129, 256, 381
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 338
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 1 cut(s) 338
FspBI CTAG 3 cut(s) 80, 341, 425
GluI GCNGC 1 cut(s) 338
GsaI CCCAGC 1 cut(s) 232
HaeIII GGCC 1 cut(s) 47
Hin1II CATG 4 cut(s) 110, 133, 260, 385
HinfI GANTC 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 2 cut(s) 117, 333
Hpy8I GTNNAC 1 cut(s) 264
HpyAV CCTTC 3 cut(s) 227, 343, 428
HpyCH4III ACNGT 5 cut(s) 67, 212, 322, 370, 404
HpyCH4IV ACGT 2 cut(s) 162, 311
HpyCH4V TGCA 2 cut(s) 4, 256
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 165, 382
HpyF3I CTNAG 3 cut(s) 222, 240, 288
HpySE526I ACGT 2 cut(s) 162, 311
Hsp92II CATG 4 cut(s) 110, 133, 260, 385
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 3 cut(s) 267, 282, 328
LmnI GCTCC 1 cut(s) 236
LpnPI CCDG 9 cut(s) 77, 104, 132, 137, 169, 214, 227, 280, 407
LweI GCATC 1 cut(s) 123
MaeI CTAG 3 cut(s) 80, 341, 425
MaeII ACGT 2 cut(s) 162, 311
MaeIII GTNAC 1 cut(s) 190
MalI GATC 3 cut(s) 269, 284, 330
MboI GATC 3 cut(s) 267, 282, 328
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 144, 241
MnlI CCTC 4 cut(s) 19, 123, 217, 318
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 133, 165, 382
NcoI CCATGG 1 cut(s) 106
NdeII GATC 3 cut(s) 267, 282, 328
NlaIII CATG 4 cut(s) 110, 133, 260, 385
NlaIV GGNNCC 2 cut(s) 104, 392
NmeAIII GCCGAG 1 cut(s) 207
NspI RCATGY 2 cut(s) 260, 385
PaeI GCATGC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 312
PmlI CACGTG 1 cut(s) 312
Ppu21I YACGTR 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 391
Psp124BI GAGCTC 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 391
Psp6I CCWGG 1 cut(s) 90
PspCI CACGTG 1 cut(s) 312
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 104, 392
PspPI GGNCC 3 cut(s) 46, 110, 391
PspPPI RGGWCCY 1 cut(s) 391
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SacI GAGCTC 1 cut(s) 241
SatI GCNGC 1 cut(s) 338
Sau3AI GATC 3 cut(s) 267, 282, 328
Sau96I GGNCC 3 cut(s) 46, 110, 391
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 144, 241
SetI ASST 8 cut(s) 78, 93, 165, 241, 314, 365, 396, 430
SfaNI GCATC 1 cut(s) 123
SinI GGWCC 2 cut(s) 110, 391
SphI GCATGC 1 cut(s) 385
SsiI CCGC 2 cut(s) 136, 338
SspMI CTAG 3 cut(s) 80, 341, 425
SstI GAGCTC 1 cut(s) 241
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 3 cut(s) 49, 106, 386
TaaI ACNGT 5 cut(s) 67, 212, 322, 370, 404
TaiI ACGT 2 cut(s) 165, 314
TaqI TCGA 1 cut(s) 177
TatI WGTACW 1 cut(s) 33
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 1 cut(s) 152
TscAI CASTG 2 cut(s) 327, 373
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 2 cut(s) 327, 373
VpaK11BI GGWCC 2 cut(s) 110, 391
XceI RCATGY 2 cut(s) 260, 385
XspI CTAG 3 cut(s) 80, 341, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.