Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003507

Physical Location & Seq

Genomic Coordinates Forward (+)

25453295 .. 25457156

Gene Structure 3862 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1163100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGCTGCTGAGCGAAATGGCTATGGAGATTACTCAGTTTCTATTGGCAGCTCAATCAGCTGATGCGAGAGTTCGTACTGAAGCAGAGGCTAATCTCAGGCAATTTCAAGAGCAAAACCTTCCTGCTTTTCTTCTATCGTTGTCCGTTGAGCTTGCAAACAATGAGAAACCCACTGAATCCCGTACGTTGGCTGGTCTTGTGCTTAAGAATTCATTAGATGCCAAAGATGCTGGTACAAAAGAACTTCTTACTCGACAATGGATGGCTATTGATATTTCTATTATATCCCAAATCAAGGACTTGCTCTTAAGGACACTTGGGTCACCTGTCCTGGAGGCAAGACACACATCTGCCCAAGTTATTGCAAAAGTTGCTTCTATTGATATTCCCAGAAAACAATGGCCCGACCTTATTGGGTCTTTGCTCGACAATATGACTCAGCGAGATAACCCAGCTGGTTTGAAGCAGTCAACTTTGGAAGCACTTGGTTATGTATGTGAGGAGATATCTCATCAAGATCTTGAGCAAGGCGAGGTGAACAATGTTCTTACTGCTGTAGTCCAAGGAATGAACCTTGCTGAAAATAGCCCGGAAGTTCGTCTGGCTGCAACAAGGGCTTTATATAATGCCCTGGAGTTTGCACAAACCAACTTTGACACTCAGATGGAACGAGATTTCATTATGAAGATGGTCTGCGAGACGGCCTTGTCTAAGGAGGTACAAATTAGGCAGGCTGCTTTTGAGTGTCTTGCTTCAATTGCCTCCAGATATTATGAGGTGCTTGAGCCTTACATGCAGGCTCTCTTTGAGCTTACATCAAATGCTATAAAAGGGGATGAAGAGGCAGTTGCCCTTCAAGCAATTGAGTTTTGGAGCTCCATTTGTGATGAAGAAATAGAGCTTCAAGAATTTGAGATCTCTGACACTGGGGACTCGGGGCCTCATTCAAAATTTATAGAAAAGGCTCTGGCGTCTTTAGTTCCATTGTTGCTAGAAACTTTGCTGAAACAGGAAGAAGATCTAGACCAAGATGACAACATTTGGAATATTGCAATGGCAGGTGGGACATGCCTACAACTTATTTCCATGACTGTTGGAGATGCCATTCTGCCCCTTGTGATGCCATTTGTGGAGGCTAACATAGTGAAGCCAGATTGGCACTGTCGTGAGGCAGCTACATTTGCATTTGGCTCAGTACTTGAAGGCCCAAGCACTGAGAAGCTCTCTGTTTTGGTTCATTCAGGTTTGGATTTCCTGCTTAGGTTAATGAAAGATGAAAACAACCATGTCAAAGACACTACTGCCTGGACTCTCAGTCGTATATTTGAGTTTTTGCATAGTCCTGGTAGTACTGTGATTTCTTCTGCTACCCTTCCAAGGGTGGTGGAAATATTGGTGGAAGGCACTAAGGATGCTCCAAATGTTGCAGAGAAGGTCTGTTGGGCCATCTATCACCTTTCTCAGGGGTATGAGGAAACCTGCTCCTCTCTATTTACTCCATATGTCCCAGGCATCATTGAATGTCTTCTTTCCACTGCTAGTCGTGCAGATGGTGATGATTCTAGGTTAAGGTCTTCTGCTTATGAATCGTTGAATGCCGTTGTGAGGTGTTCTAATCTTAAAGAAACATCACAAATTATAGCACAGCTACTTGTTGTTATTATGAATAAGTTGGGCCAGACTTTAGAACTTTCGTCAGATGACAAGGAAAAGCAAGGAGACTTGCAAGCTTCTTTTTGTGGTGTTCTACAGGTTATTATTCAGAAGCTTACCAGTGATGATGGAACTAAGCGTTTTATATTGGAAGCCGCTGATCAGATAATGGTTCTGTTTCTTAGAGTGTTTGCTTGCCGTAGTTCCACAGTACATGAGGAAGCAATGCTTGCTATAGGTGCACTGGCTTATGCAACTGGAACTGAGTTTGAAAAGTACCTTCCAGAATTGTACAAGTATTTGGAGATGGGGTTGCAGAATTTTGAGGAGTTTCAAGTATGTGCTATCACAGTTGGGGTGGTTGGTGACATTTTTCGTGCATTGGATGAGAAGGCATTACCATACTGTGACGGGATTATGAGCCACCTGATGAAGGACTTGTCTAGCGAATCGCTTCACCGGTCTGTCAAGCCTCCCATATTCTCTGTCTTTGGCGACATAGGTCTCGCAATTGGAGTACATTTTGAGAAGTATGTCCCTTATGCAGTGCAGATGATGCAGGGAGCTTCAGAGCTTTGTGCTCGGATGGACACTAGTGATGAGGAGTTAATGGAATATGGAAACCAGTTAAAGTGTAGCATCTTTGAAGCTTATTCTGGTATACTTCAGGGCTTTAAGGATTCTAAGCCTGAGGTCATGTTGCCATATGCCCAACATCTCCTGCAGTTTATAGAATTGGTTTTAAGGGATGTAAACAGGGATGCCAATGTCACAAAAGCTACAGTTGCTGCGCTGGGTGACCTTGTTGATGTACTTGGCCTAACCATCAAGCCACTGTTCGGAGATTTTGCATTCTTTGAGGGAATTTTGCAAGAGTGTCTTCAGTTCGACGATAAAAGTCTCAGGGAAACAACGGCTTGGGCTTATGCTATTATGAGGCGCATCATGGATCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N

Protein Analysis

870

Amino Acids

96.53

Weight (kDa)

4.6

Isoelectric Point (pI)

43.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	28 - 108	7.6e-10	Importin-beta N-terminal domain
TPR_IPO5	PF25780	113 - 271	9.3e-10	IPO5-like, TPR repeats
HEAT_EZ	PF13513	386 - 441	8.4e-11	HEAT-like repeat
TPR_IMB1	PF25574	461 - 823	1e-97	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003507)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08947 AT3G08947
fragaria_vesca	FvH4_6g10890 FvH4_6g10890 FvH4_6g10890 FvH4_6g10970
malus_domestica	MD04G1163100.v1.1 MD12G1176600.v1.1
prunus_persica	Prupe.6G282600_v2.0.a1 Prupe.6G282600_v2.0.a1
pyrus_communis	pycom04g14590 pycom04g14600 pycom12g16660 pycom12g16670
rosa_chinensis	RchiOBHm_Chr3g0462121 RchiOBHm_Chr3g0462271
rosa_laevigata	RLG00000024872 RLG00000024881
rosa_multiflora	Rmu_sc0006188.1_g000001 Rmu_sc0007627.1_g000010 Rmu_sc0020622.1_g000002 Rmu_sc0031852.1_g000001
rosa_roxburghii	Rroxscaffold_6G00417630 Rroxscaffold_6G00417720
rosa_rugosa	Rorug03G0055400
rosa_samantha	Rh3AG113300 Rh3AG114100 Rh3BG116200 Rh3BG117200 Rh3CG119000 Rh3CG120000 Rh3DG118100 Rh3DG119000
rosa_wichuraiana	Rw3G009560 Rw3G009630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1049
AasI	GACNNNNNNGTC	2 cut(s)	319, 706
Acc36I	ACCTGC	2 cut(s)	1049, 1487
AccI	GTMKAC	1 cut(s)	2314
AciI	CCGC	1 cut(s)	1809
AcsI	RAATTY	5 cut(s)	208, 908, 950, 1972, 2517
AcuI	CTGAAG	4 cut(s)	99, 2205, 2303, 2519
AcyI	GRCGYC	1 cut(s)	971
AfiI	CCNNNNNNNGG	8 cut(s)	187, 295, 1377, 1378, 1462, 1605, 2086, 2492
AflII	CTTAAG	2 cut(s)	203, 307
AgeI	ACCGGT	1 cut(s)	2112
AhdI	GACNNNNNGTC	1 cut(s)	1314
AhlI	ACTAGT	1 cut(s)	2246
AjnI	CCWGG	5 cut(s)	330, 630, 1304, 1342, 1507
AleI	CACNNNNGTG	1 cut(s)	1164
Alw21I	GWGCWC	3 cut(s)	878, 1897, 2236
Alw26I	GTCTC	4 cut(s)	692, 1713, 2162, 2558
Alw44I	GTGCAC	1 cut(s)	1893
AlwNI	CAGNNNCTG	1 cut(s)	2441
Ama87I	CYCGRG	1 cut(s)	934
AoxI	GGCC	7 cut(s)	401, 702, 938, 1204, 1443, 1675, 2470
ApaLI	GTGCAC	1 cut(s)	1893
ApeKI	GCWGC	6 cut(s)	4, 47, 605, 734, 1172, 2441
ApoI	RAATTY	5 cut(s)	208, 908, 950, 1972, 2517
AsiGI	ACCGGT	1 cut(s)	2112
Asp700I	GAANNNNTTC	2 cut(s)	1524, 2106
AspLEI	GCGC	2 cut(s)	2446, 2595
AspS9I	GGNCC	5 cut(s)	402, 938, 1205, 1443, 1675
AsuC2I	CCSGG	1 cut(s)	590
AsuHPI	GGTGA	7 cut(s)	315, 547, 1445, 1565, 2030, 2102, 2462
AvaI	CYCGRG	1 cut(s)	934
AxyI	CCTNAGG	1 cut(s)	2343
BaeGI	GKGCMC	1 cut(s)	1897
BanII	GRGCYC	1 cut(s)	878
BbsI	GAAGAC	3 cut(s)	1517, 1566, 2525
Bbv12I	GWGCWC	3 cut(s)	878, 1897, 2236
BbvI	GCAGC	5 cut(s)	59, 592, 721, 1184, 2428
BccI	CCATC	9 cut(s)	256, 658, 682, 1454, 1544, 1775, 1954, 2233, 2486
BceAI	ACGGC	4 cut(s)	717, 1583, 1836, 2583
BciT130I	CCWGG	5 cut(s)	332, 632, 1306, 1344, 1509
BclI	TGATCA	1 cut(s)	1813
BcnI	CCSGG	1 cut(s)	590
BcoDI	GTCTC	4 cut(s)	692, 1713, 2162, 2558
BcuI	ACTAGT	1 cut(s)	2246
BfaI	CTAG	6 cut(s)	992, 1022, 1539, 1563, 2097, 2247
BfmI	CTRYAG	5 cut(s)	555, 1748, 1887, 2375, 2433
BfrI	CTTAAG	2 cut(s)	203, 307
BfuAI	ACCTGC	2 cut(s)	1049, 1487
BglI	GCCNNNNNGGC	1 cut(s)	1156
BglII	AGATCT	3 cut(s)	517, 915, 1018
BisI	GCNGC	7 cut(s)	5, 48, 606, 735, 1173, 1809, 2442
BlpI	GCTNAGC	1 cut(s)	8
BlsI	GCNGC	7 cut(s)	6, 49, 607, 736, 1174, 1810, 2443
BmcAI	AGTACT	2 cut(s)	1197, 1351
Bme1390I	CCNGG	6 cut(s)	332, 590, 632, 1306, 1344, 1509
BmeRI	GACNNNNNGTC	1 cut(s)	1314
BmeT110I	CYCGRG	1 cut(s)	934
BmgT120I	GGNCC	5 cut(s)	402, 938, 1205, 1443, 1675
BmiI	GGNNCC	1 cut(s)	939
BmrFI	CCNGG	6 cut(s)	332, 590, 632, 1306, 1344, 1509
BmrI	ACTGGG	1 cut(s)	936
BmuI	ACTGGG	1 cut(s)	936
BoxI	GACNNNNGTC	1 cut(s)	2154
BpiI	GAAGAC	3 cut(s)	1517, 1566, 2525
BplI	GAGNNNNNCTC	2 cut(s)	1208, 1240
BpmI	CTGGAG	3 cut(s)	353, 653, 748
Bpu10I	CCTNAGC	1 cut(s)	1259
Bpu1102I	GCTNAGC	1 cut(s)	8
BpuEI	CTTGAG	2 cut(s)	542, 803
BpuMI	CCSGG	1 cut(s)	590
BsaHI	GRCGYC	1 cut(s)	971
BsaI	GGTCTC	1 cut(s)	2162
BsaJI	CCNNGG	4 cut(s)	562, 630, 1376, 1507
BsaWI	WCCGGW	1 cut(s)	2112
BsaXI	ACNNNNNCTCC	2 cut(s)	1949, 1979
Bsc4I	CCNNNNNNNGG	8 cut(s)	187, 295, 1377, 1378, 1462, 1605, 2086, 2492
Bse118I	RCCGGY	1 cut(s)	2112
Bse1I	ACTGG	5 cut(s)	931, 1773, 1902, 1915, 2278
Bse21I	CCTNAGG	1 cut(s)	2343
Bse3DI	GCAATG	2 cut(s)	1059, 1884
BseBI	CCWGG	5 cut(s)	332, 632, 1306, 1344, 1509
BseDI	CCNNGG	4 cut(s)	562, 630, 1376, 1507
BseGI	GGATG	7 cut(s)	267, 841, 1417, 2044, 2244, 2407, 2419
BseLI	CCNNNNNNNGG	8 cut(s)	187, 295, 1377, 1378, 1462, 1605, 2086, 2492
BseMI	GCAATG	2 cut(s)	1059, 1884
BseNI	ACTGG	5 cut(s)	931, 1773, 1902, 1915, 2278
BseRI	GAGGAG	4 cut(s)	515, 1474, 1994, 2270
BseSI	GKGCMC	1 cut(s)	1897
BseXI	GCAGC	5 cut(s)	59, 592, 721, 1184, 2428
BseYI	CCCAGC	2 cut(s)	451, 2446
BsgI	GTGCAG	2 cut(s)	1566, 2222
BshFI	GGCC	7 cut(s)	403, 704, 940, 1206, 1445, 1677, 2472
BshTI	ACCGGT	1 cut(s)	2112
BsiHKAI	GWGCWC	3 cut(s)	878, 1897, 2236
BsiHKCI	CYCGRG	1 cut(s)	934
BsiSI	CCGG	2 cut(s)	590, 2113
BsiWI	CGTACG	1 cut(s)	182
BslFI	GGGAC	4 cut(s)	944, 1078, 1490, 2174
BslI	CCNNNNNNNGG	8 cut(s)	187, 295, 1377, 1378, 1462, 1605, 2086, 2492
BsmAI	GTCTC	4 cut(s)	692, 1713, 2162, 2558
BsmBI	CGTCTC	1 cut(s)	692
BsmFI	GGGAC	4 cut(s)	944, 1078, 1490, 2174
BsmI	GAATGC	2 cut(s)	1600, 2504
BsnI	GGCC	7 cut(s)	403, 704, 940, 1206, 1445, 1677, 2472
Bso31I	GGTCTC	1 cut(s)	2162
BsoBI	CYCGRG	1 cut(s)	934
Bsp1286I	GDGCHC	3 cut(s)	878, 1897, 2236
Bsp1407I	TGTACA	1 cut(s)	1944
Bsp143I	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
Bsp1720I	GCTNAGC	1 cut(s)	8
BspACI	CCGC	1 cut(s)	1809
BspANI	GGCC	7 cut(s)	403, 704, 940, 1206, 1445, 1677, 2472
BspLI	GGNNCC	1 cut(s)	939
BspMAI	CTGCAG	1 cut(s)	2379
BspMI	ACCTGC	2 cut(s)	1049, 1487
BspTI	CTTAAG	2 cut(s)	203, 307
BspTNI	GGTCTC	1 cut(s)	2162
BsrDI	GCAATG	2 cut(s)	1059, 1884
BsrFI	RCCGGY	1 cut(s)	2112
BsrGI	TGTACA	1 cut(s)	1944
BsrI	ACTGG	5 cut(s)	931, 1773, 1902, 1915, 2278
BssAI	RCCGGY	1 cut(s)	2112
BssECI	CCNNGG	4 cut(s)	562, 630, 1376, 1507
BssMI	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
BssNAI	GTATAC	1 cut(s)	2315
BssNI	GRCGYC	1 cut(s)	971
BssT1I	CCWWGG	2 cut(s)	562, 1376
Bst1107I	GTATAC	1 cut(s)	2315
Bst2UI	CCWGG	5 cut(s)	332, 632, 1306, 1344, 1509
Bst4CI	ACNGT	8 cut(s)	1093, 1163, 1354, 1864, 2005, 2060, 2437, 2490
Bst6I	CTCTTC	1 cut(s)	834
BstACI	GRCGYC	1 cut(s)	971
BstAFI	CTTAAG	2 cut(s)	203, 307
BstAPI	GCANNNNNTGC	3 cut(s)	371, 1883, 2209
BstAUI	TGTACA	1 cut(s)	1944
BstC8I	GCNNGC	6 cut(s)	153, 732, 798, 1728, 1849, 1884
BstEII	GGTNACC	2 cut(s)	321, 2450
BstENI	CCTNNNNNAGG	2 cut(s)	1460, 2084
BstF5I	GGATG	7 cut(s)	267, 841, 1417, 2044, 2244, 2407, 2419
BstHHI	GCGC	2 cut(s)	2446, 2595
BstKTI	GATC	5 cut(s)	520, 918, 1021, 1816, 2605
BstMAI	GTCTC	4 cut(s)	692, 1713, 2162, 2558
BstMBI	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
BstNI	CCWGG	5 cut(s)	332, 632, 1306, 1344, 1509
BstNSI	RCATGY	2 cut(s)	796, 1071
BstPAI	GACNNNNGTC	1 cut(s)	2154
BstPI	GGTNACC	2 cut(s)	321, 2450
BstSCI	CCNGG	6 cut(s)	330, 588, 630, 1304, 1342, 1507
BstSFI	CTRYAG	5 cut(s)	555, 1748, 1887, 2375, 2433
BstSLI	GKGCMC	1 cut(s)	1897
BstV1I	GCAGC	5 cut(s)	59, 592, 721, 1184, 2428
BstV2I	GAAGAC	3 cut(s)	1517, 1566, 2525
BstX2I	RGATCY	3 cut(s)	517, 915, 1018
BstYI	RGATCY	3 cut(s)	517, 915, 1018
BstZ17I	GTATAC	1 cut(s)	2315
Bsu36I	CCTNAGG	1 cut(s)	2343
BsuRI	GGCC	7 cut(s)	403, 704, 940, 1206, 1445, 1677, 2472
BtsCI	GGATG	7 cut(s)	267, 841, 1417, 2044, 2244, 2407, 2419
BtsI	GCAGTG	2 cut(s)	1533, 2205
BtsIMutI	CAGTG	9 cut(s)	171, 924, 1159, 1212, 1533, 1780, 1895, 2205, 2486
BveI	ACCTGC	2 cut(s)	1049, 1487
Cac8I	GCNNGC	6 cut(s)	153, 732, 798, 1728, 1849, 1884
CaiI	CAGNNNCTG	1 cut(s)	2441
CfoI	GCGC	2 cut(s)	2446, 2595
Cfr10I	RCCGGY	1 cut(s)	2112
Cfr13I	GGNCC	5 cut(s)	402, 938, 1205, 1443, 1675
CseI	GACGC	1 cut(s)	960
CspAI	ACCGGT	1 cut(s)	2112
CspCI	CAANNNNNGTGG	2 cut(s)	1365, 1400
CviAII	CATG	7 cut(s)	793, 1068, 1087, 1286, 1868, 2350, 2599
DpnI	GATC	5 cut(s)	519, 917, 1020, 1815, 2604
DpnII	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
DrdI	GACNNNNNNGTC	2 cut(s)	319, 706
DriI	GACNNNNNGTC	1 cut(s)	1314
DseDI	GACNNNNNNGTC	2 cut(s)	319, 706
Eam1104I	CTCTTC	1 cut(s)	834
Eam1105I	GACNNNNNGTC	1 cut(s)	1314
EarI	CTCTTC	1 cut(s)	834
Ecl136II	GAGCTC	1 cut(s)	876
Eco130I	CCWWGG	2 cut(s)	562, 1376
Eco24I	GRGCYC	1 cut(s)	878
Eco31I	GGTCTC	1 cut(s)	2162
Eco32I	GATATC	1 cut(s)	507
Eco53kI	GAGCTC	1 cut(s)	876
Eco57I	CTGAAG	4 cut(s)	99, 2205, 2303, 2519
Eco81I	CCTNAGG	1 cut(s)	2343
Eco88I	CYCGRG	1 cut(s)	934
Eco91I	GGTNACC	2 cut(s)	321, 2450
EcoICRI	GAGCTC	1 cut(s)	876
EcoNI	CCTNNNNNAGG	2 cut(s)	1460, 2084
EcoO109I	RGGNCCY	1 cut(s)	938
EcoO65I	GGTNACC	2 cut(s)	321, 2450
EcoRI	GAATTC	1 cut(s)	208
EcoRII	CCWGG	5 cut(s)	330, 630, 1304, 1342, 1507
EcoRV	GATATC	1 cut(s)	507
EcoT14I	CCWWGG	2 cut(s)	562, 1376
EcoT38I	GRGCYC	1 cut(s)	878
ErhI	CCWWGG	2 cut(s)	562, 1376
Esp3I	CGTCTC	1 cut(s)	692
FaeI	CATG	7 cut(s)	796, 1071, 1090, 1289, 1871, 2353, 2602
FalI	AAGNNNNNCTT	6 cut(s)	231, 263, 1866, 1898, 2517, 2549
FaqI	GGGAC	4 cut(s)	944, 1078, 1490, 2174
FatI	CATG	7 cut(s)	792, 1067, 1086, 1285, 1867, 2349, 2598
FauNDI	CATATG	2 cut(s)	1501, 2359
FbaI	TGATCA	1 cut(s)	1813
FblI	GTMKAC	1 cut(s)	2314
Fnu4HI	GCNGC	7 cut(s)	5, 48, 606, 735, 1173, 1809, 2442
FokI	GGATG	7 cut(s)	274, 848, 1424, 2051, 2251, 2414, 2426
FriOI	GRGCYC	1 cut(s)	878
Fsp4HI	GCNGC	7 cut(s)	5, 48, 606, 735, 1173, 1809, 2442
FspBI	CTAG	6 cut(s)	992, 1022, 1539, 1563, 2097, 2247
GlaI	GCGC	2 cut(s)	2445, 2594
GluI	GCNGC	7 cut(s)	5, 48, 606, 735, 1173, 1809, 2442
GsaI	CCCAGC	2 cut(s)	455, 2450
GsuI	CTGGAG	3 cut(s)	353, 653, 748
HaeIII	GGCC	7 cut(s)	403, 704, 940, 1206, 1445, 1677, 2472
HapII	CCGG	2 cut(s)	590, 2113
HgaI	GACGC	1 cut(s)	960
HhaI	GCGC	2 cut(s)	2446, 2595
Hin1I	GRCGYC	1 cut(s)	971
Hin1II	CATG	7 cut(s)	796, 1071, 1090, 1289, 1871, 2353, 2602
Hin6I	GCGC	2 cut(s)	2444, 2593
HinP1I	GCGC	2 cut(s)	2444, 2593
HincII	GTYRAC	1 cut(s)	471
HindII	GTYRAC	1 cut(s)	471
HindIII	AAGCTT	3 cut(s)	1728, 1766, 2301
HinfI	GANTC	8 cut(s)	176, 436, 932, 1309, 1559, 1586, 2102, 2333
HpaII	CCGG	2 cut(s)	590, 2113
HphI	GGTGA	7 cut(s)	315, 547, 1445, 1565, 2030, 2102, 2462
Hpy166II	GTNNAC	5 cut(s)	471, 538, 1895, 2315, 2407
Hpy188I	TCNGA	8 cut(s)	663, 922, 1699, 1764, 1818, 2224, 2238, 2495
Hpy188III	TCNNGA	8 cut(s)	107, 515, 521, 765, 905, 1022, 1166, 1937
Hpy8I	GTNNAC	5 cut(s)	471, 538, 1895, 2315, 2407
Hpy99I	CGWCG	1 cut(s)	2546
HpyAV	CCTTC	9 cut(s)	128, 863, 1196, 1382, 1394, 1426, 1943, 2038, 2080
HpyCH4III	ACNGT	8 cut(s)	1093, 1163, 1354, 1864, 2005, 2060, 2437, 2490
HpyCH4IV	ACGT	1 cut(s)	185
HpySE526I	ACGT	1 cut(s)	185
Hsp92I	GRCGYC	1 cut(s)	971
Hsp92II	CATG	7 cut(s)	796, 1071, 1090, 1289, 1871, 2353, 2602
HspAI	GCGC	2 cut(s)	2444, 2593
Ksp22I	TGATCA	1 cut(s)	1813
Kzo9I	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
LmnI	GCTCC	5 cut(s)	873, 881, 1420, 1487, 2216
Lsp1109I	GCAGC	5 cut(s)	59, 592, 721, 1184, 2428
MaeI	CTAG	6 cut(s)	992, 1022, 1539, 1563, 2097, 2247
MaeII	ACGT	1 cut(s)	185
MaeIII	GTNAC	5 cut(s)	321, 2018, 2060, 2422, 2450
MalI	GATC	5 cut(s)	519, 917, 1020, 1815, 2604
MboI	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
MfeI	CAATTG	3 cut(s)	756, 861, 2163
MflI	RGATCY	3 cut(s)	517, 915, 1018
MhlI	GDGCHC	3 cut(s)	878, 1897, 2236
MlyI	GAGTC	3 cut(s)	430, 926, 1303
MmeI	TCCRAC	1 cut(s)	1075
MroXI	GAANNNNTTC	2 cut(s)	1524, 2106
MseI	TTAA	9 cut(s)	204, 308, 1265, 1568, 1620, 2261, 2282, 2328, 2396
MslI	CAYNNNNRTG	2 cut(s)	662, 1164
MspA1I	CMGCKG	3 cut(s)	59, 455, 1811
MspCI	CTTAAG	2 cut(s)	203, 307
MspI	CCGG	2 cut(s)	590, 2113
MspR9I	CCNGG	6 cut(s)	332, 590, 632, 1306, 1344, 1509
MunI	CAATTG	3 cut(s)	756, 861, 2163
Mva1269I	GAATGC	2 cut(s)	1600, 2504
MvaI	CCWGG	5 cut(s)	332, 632, 1306, 1344, 1509
NciI	CCSGG	1 cut(s)	590
NdeI	CATATG	2 cut(s)	1501, 2359
NdeII	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
NlaIII	CATG	7 cut(s)	796, 1071, 1090, 1289, 1871, 2353, 2602
NlaIV	GGNNCC	1 cut(s)	939
NmuCI	GTSAC	5 cut(s)	321, 2018, 2060, 2422, 2450
NspI	RCATGY	2 cut(s)	796, 1071
OliI	CACNNNNGTG	1 cut(s)	1164
PaqCI	CACCTGC	1 cut(s)	1049
PctI	GAATGC	2 cut(s)	1600, 2504
PdmI	GAANNNNTTC	2 cut(s)	1524, 2106
PfeI	GAWTC	5 cut(s)	176, 1559, 1586, 2102, 2333
Pfl23II	CGTACG	1 cut(s)	182
PfoI	TCCNGGA	1 cut(s)	330
PinAI	ACCGGT	1 cut(s)	2112
PkrI	GCNGC	7 cut(s)	6, 49, 607, 736, 1174, 1810, 2443
PleI	GAGTC	3 cut(s)	430, 926, 1303
PpsI	GAGTC	3 cut(s)	430, 926, 1303
PshAI	GACNNNNGTC	1 cut(s)	2154
Psp124BI	GAGCTC	1 cut(s)	878
Psp6I	CCWGG	5 cut(s)	330, 630, 1304, 1342, 1507
PspEI	GGTNACC	2 cut(s)	321, 2450
PspFI	CCCAGC	2 cut(s)	451, 2446
PspGI	CCWGG	5 cut(s)	330, 630, 1304, 1342, 1507
PspLI	CGTACG	1 cut(s)	182
PspN4I	GGNNCC	1 cut(s)	939
PspPI	GGNCC	5 cut(s)	402, 938, 1205, 1443, 1675
PstI	CTGCAG	1 cut(s)	2379
PstNI	CAGNNNCTG	1 cut(s)	2441
PsuI	RGATCY	3 cut(s)	517, 915, 1018
PvuII	CAGCTG	2 cut(s)	59, 455
RseI	CAYNNNNRTG	2 cut(s)	662, 1164
SacI	GAGCTC	1 cut(s)	878
SaqAI	TTAA	9 cut(s)	204, 308, 1265, 1568, 1620, 2261, 2282, 2328, 2396
SatI	GCNGC	7 cut(s)	5, 48, 606, 735, 1173, 1809, 2442
Sau3AI	GATC	5 cut(s)	517, 915, 1018, 1813, 2602
Sau96I	GGNCC	5 cut(s)	402, 938, 1205, 1443, 1675
ScaI	AGTACT	2 cut(s)	1197, 1351
SchI	GAGTC	3 cut(s)	430, 926, 1303
ScrFI	CCNGG	6 cut(s)	332, 590, 632, 1306, 1344, 1509
SduI	GDGCHC	3 cut(s)	878, 1897, 2236
SfcI	CTRYAG	5 cut(s)	555, 1748, 1887, 2375, 2433
SmiMI	CAYNNNNRTG	2 cut(s)	662, 1164
SmlI	CTYRAG	4 cut(s)	203, 307, 521, 782
SmoI	CTYRAG	4 cut(s)	203, 307, 521, 782
SpeI	ACTAGT	1 cut(s)	2246
SsiI	CCGC	1 cut(s)	1809
SspI	AATATT	2 cut(s)	1048, 1392
SspMI	CTAG	6 cut(s)	992, 1022, 1539, 1563, 2097, 2247
SstI	GAGCTC	1 cut(s)	878
StyD4I	CCNGG	6 cut(s)	330, 588, 630, 1304, 1342, 1507
StyI	CCWWGG	2 cut(s)	562, 1376
TaaI	ACNGT	8 cut(s)	1093, 1163, 1354, 1864, 2005, 2060, 2437, 2490
TaiI	ACGT	1 cut(s)	188
TaqI	TCGA	3 cut(s)	253, 426, 2541
TatI	WGTACW	6 cut(s)	1195, 1349, 1864, 1944, 2170, 2464
TauI	GCSGC	1 cut(s)	1811
TfiI	GAWTC	5 cut(s)	176, 1559, 1586, 2102, 2333
Tru1I	TTAA	9 cut(s)	204, 308, 1265, 1568, 1620, 2261, 2282, 2328, 2396
Tru9I	TTAA	9 cut(s)	204, 308, 1265, 1568, 1620, 2261, 2282, 2328, 2396
TscAI	CASTG	9 cut(s)	178, 931, 1166, 1219, 1540, 1780, 1902, 2205, 2493
TseFI	GTSAC	5 cut(s)	321, 2018, 2060, 2422, 2450
TseI	GCWGC	6 cut(s)	4, 47, 605, 734, 1172, 2441
Tsp45I	GTSAC	5 cut(s)	321, 2018, 2060, 2422, 2450
TspGWI	ACGGA	1 cut(s)	133
TspRI	CASTG	9 cut(s)	178, 931, 1166, 1219, 1540, 1780, 1902, 2205, 2493
Vha464I	CTTAAG	2 cut(s)	203, 307
VneI	GTGCAC	1 cut(s)	1893
XagI	CCTNNNNNAGG	2 cut(s)	1460, 2084
XapI	RAATTY	5 cut(s)	208, 908, 950, 1972, 2517
XbaI	TCTAGA	1 cut(s)	1021
XceI	RCATGY	2 cut(s)	796, 1071
XmiI	GTMKAC	1 cut(s)	2314
XmnI	GAANNNNTTC	2 cut(s)	1524, 2106
XspI	CTAG	6 cut(s)	992, 1022, 1539, 1563, 2097, 2247
ZrmI	AGTACT	2 cut(s)	1197, 1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.