RLG00000024881

HEAT-like repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003507
Physical Location & Seq
Reverse (-)
40021234 .. 40024082
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024881

Sequence Viewer

Length: 2271 bp
ATGGCCATGGAGATCACTCAGTATCTATTGTCAGCTCAATCAGCCGATGCAAGAGTTCGTACTGAAGCAGAATCTAATCTGAGGAAATTTCAAGAGCAAAACCTACCTGCTTTCCTTCTGTTGTTGTCGGTTGAGCTAGCAAACAATGAAAAGCCAGTTGAATCCCGAAGGTTGGCTGGTATTGTTCTTAAGAACTTACTAGATGTCAAAGATTCTCCTACAAAGAAACATCTTGCTCAGCAATGGATGGCTATTGATATTGCATTTAAATCCCAAATCAAGAGCTGGCTCTTACAGACACTTGGGTCATCTGTGCCAGAGGCAAGGCGCACATCTGCCCAAGTTCTTGCCAAAGTTGCTTCTATTGATGTTCCCCGGAAACAGTGGCCTGAGCTAATCGGACATTTGCTTAATAACATGATTCAGCGCGATAGCTCTGCTGATTTGAAACAGTCAACCTTGGAAACTCTTGGTTATGTATGTGAGGAAATATCCCATCCTGATCTTCAGCAAGATGAGGTAAACTCTGTTCTTACAGCTGTACTTCAAGGAATGAATCTTGCTGAGAACAGTTCTGAAGTCCGTCTTGCTGCAACAAGGGCATTATGTAATGCCTTGGAGTTTGCTCAGACAAACTTTGAAAATGAGACGGAACGAACTTGCATCATGAAGATGATCTGTGAGACTGCCTTGTCTAAGGAGGTAGAGGTTAGGCAGGCTGCTTTTGAGTGCCTTGCTTCAATTGCTTCTATGTACTATTCTGTGCTTGGGACTTACATGCAGGCTCTCTTTGAGCTTACATCAAATGCAGTAAAAGGGGATGTAGAACCTGTTGCCCTTCAAGCAATTGAGTTCTGGAGTTCCATTTGTGAAAAAGAAATAGAATTGCAAAAGTATAAGACAGCTGACAGGAACTGTCGGGACTCTTTACCGGACTTTGTGCCTCCTCATTCACGTTTTATAGAAAAATCTCAAATTTCTCTAATTCCAATATTGCTAGAGACTTTGCTGATGCAGGAAGAAAATGTAAGCCAGGATGACAACATTTGGAATATCTCAATGGCTGGTAAGAAATGCCTAGAACTTGTTGCCCAAACTGTTGGGTTTGTCATTGTACCCCTCGTGATTCCATTTGTGGTGGCTAACAGAGTGAAACCAGATTGGCATTGTCGGGAGGCAGCAGTTTTTGCACTTGGTGCAATTATTGAAGGCTCAACCATTTATCAGGATCTTGGGGTCTTATTAAAATACTGTGAACTGGTCCGTGAACTTATAGATGACTTGCTTTTCTTAATGAATGATGAAAACAACCAAGTCAAAGAAACTACTGCATGGACTCTTGGTCGTATATTTGAATTTTTGCATAGTCCTGTTTGTGGATTTTCTGTGATTACTTCTGATAATCTTCCAAGAGTGGTAGATATATTGGTGAAGAGCGTTAAGGATGCTCCAAATGTTGCAACTAAGGTGTGTCGGGTGATTTATACACTTGCTGAGGGCTATGAGGAAGCTGGAATGCACTCATCTTTTTTTACGCCATATATCCCTAGGATCATTGAAAGTCTTCTTTTTGCTGCTAGTTGTGCAGATTTGGATGATTCGAAATTGAGGTCTGCTGCATATGAATCATTAAACGAGGTAGTGAGGTGTTCCAGCAGTACCGAAACACCACAGATTATAAGAGAACTACTCTCTGTGATTTTGGAGAGGTTGAGTCAGACTTTAGAGCTTCAGATTGTATCATCAGATGGCAAGGAAAAGCAAGCAGACTTGCAGGCTTCTCTCTGTGGTGTTCTCCAGGTTATAATAGAGAAAGTTAGCAGTGCCAATGATTTAGAAACCAGGTCCATTATGTTGACACAGGCAGACCAGATAATTGAAGCACTCGACCGGTCTGTCAAGCCTCCCATATTCTCTGTCATTGGCGACATAGCTCTCTCAATAGAGGAAAACTTTCAGAAGTATGTTGACTCTGCAGTGCCAATGATGCAGAGCGCTGTGGAGCTGTGTGCTCAGATGGACTCCAATGATGATGAGTTATTGGAGTACGGCAGTCAGCTCAAGTGTAGCATCTTTGAAGCTTATTCTGGTATACTTCGGGGCACCAAGAAGGATATCCTTCTAAGAAAAGCTTTAGCTGCTGTGATGGATGACCTTGCAGATTTACTTGGTACAAACTCAAAGACACTCTTTGGAGACCTTACTGCTCGTGTAACATATACATCATATACTGGTGCGTGTCTTCAATCTGATTGTGATAACCTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

757

Amino Acids

84.09

Weight (kDa)

4.75

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBN_N PF03810 23 - 103 1e-08 Importin-beta N-terminal domain
TPR_IPO5 PF25780 106 - 266 1.8e-08 IPO5-like, TPR repeats
HEAT_EZ PF13513 388 - 450 1.6e-06 HEAT-like repeat
TPR_IMB1 PF25574 472 - 618 2.1e-23 Importin subunit beta-1-like, TPR repeats
TPR_IMB1 PF25574 627 - 719 4.1e-17 Importin subunit beta-1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1679, 1805
AasI GACNNNNNNGTC 3 cut(s) 304, 691, 1895
Acc36I ACCTGC 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 2102
AccI GTMKAC 1 cut(s) 2092
AccII CGCG 1 cut(s) 429
AclWI GGATC 2 cut(s) 1236, 1559
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 86, 975, 1355
AcuI CTGAAG 4 cut(s) 84, 491, 597, 1715
AdeI CACNNNGTG 1 cut(s) 1196
AfaI GTAC 7 cut(s) 61, 543, 755, 1116, 1660, 2048, 2173
AfeI AGCGCT 1 cut(s) 1996
AfiI CCNNNNNNNGG 2 cut(s) 172, 1376
AflII CTTAAG 1 cut(s) 188
AgeI ACCGGT 1 cut(s) 1890
AhdI GACNNNNNGTC 1 cut(s) 1341
AjnI CCWGG 3 cut(s) 1032, 1797, 1841
Alw21I GWGCWC 1 cut(s) 2014
Alw26I GTCTC 4 cut(s) 641, 677, 995, 2190
AlwI GGATC 2 cut(s) 1236, 1559
AlwNI CAGNNNCTG 1 cut(s) 915
Aor51HI AGCGCT 1 cut(s) 1996
AoxI GGCC 2 cut(s) 3, 386
ApeKI GCWGC 6 cut(s) 590, 719, 1178, 1574, 1616, 2138
ApoI RAATTY 3 cut(s) 86, 975, 1355
ArsI GACNNNNNNTTYG 2 cut(s) 1595, 1627
AsiGI ACCGGT 1 cut(s) 1890
Asp700I GAANNNNTTC 2 cut(s) 1563, 1953
AspA2I CCTAGG 1 cut(s) 1547
AspLEI GCGC 3 cut(s) 330, 429, 1997
AspS9I GGNCC 2 cut(s) 1261, 1845
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 1441, 1489
AsuII TTCGAA 1 cut(s) 1601
AsuNHI GCTAGC 1 cut(s) 136
AvaII GGWCC 2 cut(s) 1261, 1845
AvrII CCTAGG 1 cut(s) 1547
BaeGI GKGCMC 1 cut(s) 2105
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 2102
BauI CACGAG 2 cut(s) 1121, 2208
BbsI GAAGAC 2 cut(s) 1556, 2234
Bbv12I GWGCWC 1 cut(s) 2014
BbvCI CCTCAGC 1 cut(s) 1494
BbvI GCAGC 6 cut(s) 577, 706, 1190, 1561, 1603, 2125
BccI CCATC 5 cut(s) 241, 504, 1742, 2011, 2140
BceAI ACGGC 1 cut(s) 2065
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 3 cut(s) 1034, 1799, 1843
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 4 cut(s) 641, 677, 995, 2190
BfaI CTAG 6 cut(s) 137, 200, 998, 1079, 1548, 1578
BfmI CTRYAG 1 cut(s) 1974
BfoI RGCGCY 1 cut(s) 1998
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 115
BisI GCNGC 6 cut(s) 591, 720, 1179, 1575, 1617, 2139
BlnI CCTAGG 1 cut(s) 1547
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 6 cut(s) 592, 721, 1180, 1576, 1618, 2140
Bme1390I CCNGG 4 cut(s) 376, 1034, 1799, 1843
Bme18I GGWCC 2 cut(s) 1261, 1845
BmeRI GACNNNNNGTC 1 cut(s) 1341
BmgT120I GGNCC 2 cut(s) 1261, 1845
BmiI GGNNCC 1 cut(s) 2104
BmrFI CCNGG 4 cut(s) 376, 1034, 1799, 1843
BmsI GCATC 6 cut(s) 37, 672, 1002, 1435, 1977, 2079
BmtI GCTAGC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 1556, 2234
BplI GAGNNNNNCTC 4 cut(s) 509, 541, 1674, 1706
BpmI CTGGAG 2 cut(s) 877, 1781
Bpu10I CCTNAGC 2 cut(s) 390, 1494
Bpu1102I GCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 1601
BpuEI CTTGAG 1 cut(s) 2045
BpuMI CCSGG 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 2190
BsaJI CCNNGG 5 cut(s) 6, 374, 459, 615, 1547
BsaWI WCCGGW 2 cut(s) 931, 1890
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 1376
Bse118I RCCGGY 1 cut(s) 1890
Bse1I ACTGG 3 cut(s) 155, 1263, 2236
Bse3DI GCAATG 1 cut(s) 248
BseBI CCWGG 3 cut(s) 1034, 1799, 1843
BseDI CCNNGG 5 cut(s) 6, 374, 459, 615, 1547
BseGI GGATG 7 cut(s) 252, 496, 826, 1042, 1450, 1600, 2155
BseLI CCNNNNNNNGG 2 cut(s) 172, 1376
BseMI GCAATG 1 cut(s) 248
BseMII CTCAG 8 cut(s) 32, 71, 251, 381, 555, 641, 1485, 2027
BseNI ACTGG 3 cut(s) 155, 1263, 2236
BseRI GAGGAG 1 cut(s) 936
BseSI GKGCMC 1 cut(s) 2105
BseXI GCAGC 6 cut(s) 577, 706, 1190, 1561, 1603, 2125
BsgI GTGCAG 1 cut(s) 1605
Bsh1236I CGCG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 1891
BshFI GGCC 2 cut(s) 5, 388
BshNI GGYRCC 1 cut(s) 2102
BshTI ACCGGT 1 cut(s) 1890
BsiEI CGRYCG 1 cut(s) 1891
BsiHKAI GWGCWC 1 cut(s) 2014
BsiSI CCGG 3 cut(s) 376, 932, 1891
BslFI GGGAC 2 cut(s) 784, 935
BslI CCNNNNNNNGG 2 cut(s) 172, 1376
BsmAI GTCTC 4 cut(s) 641, 677, 995, 2190
BsmBI CGTCTC 1 cut(s) 641
BsmFI GGGAC 2 cut(s) 784, 935
BsmI GAATGC 1 cut(s) 1521
BsnI GGCC 2 cut(s) 5, 388
Bso31I GGTCTC 1 cut(s) 2190
Bsp119I TTCGAA 1 cut(s) 1601
Bsp1286I GDGCHC 2 cut(s) 2014, 2105
Bsp143I GATC 5 cut(s) 12, 502, 675, 1228, 1551
Bsp1720I GCTNAGC 1 cut(s) 237
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 2 cut(s) 5, 388
BspCNI CTCAG 8 cut(s) 31, 72, 250, 382, 556, 640, 1486, 2026
BspFNI CGCG 1 cut(s) 429
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 2104
BspMAI CTGCAG 1 cut(s) 1978
BspMI ACCTGC 1 cut(s) 115
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 2 cut(s) 1236, 1559
BspQI GCTCTTC 1 cut(s) 1427
BspT104I TTCGAA 1 cut(s) 1601
BspT107I GGYRCC 1 cut(s) 2102
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 2190
BsrDI GCAATG 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 1890
BsrI ACTGG 3 cut(s) 155, 1263, 2236
BssAI RCCGGY 1 cut(s) 1890
BssECI CCNNGG 5 cut(s) 6, 374, 459, 615, 1547
BssMI GATC 5 cut(s) 12, 502, 675, 1228, 1551
BssNAI GTATAC 1 cut(s) 2093
BssSI CACGAG 2 cut(s) 1121, 2208
BssT1I CCWWGG 4 cut(s) 6, 459, 615, 1547
Bst1107I GTATAC 1 cut(s) 2093
Bst2BI CACGAG 2 cut(s) 1121, 2208
Bst2UI CCWGG 3 cut(s) 1034, 1799, 1843
Bst4CI ACNGT 6 cut(s) 384, 453, 572, 917, 1099, 1253
Bst6I CTCTTC 1 cut(s) 1427
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 2 cut(s) 1187, 1196
BstBI TTCGAA 1 cut(s) 1601
BstC8I GCNNGC 6 cut(s) 138, 287, 717, 783, 1764, 1776
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 7 cut(s) 252, 496, 826, 1042, 1450, 1600, 2155
BstFNI CGCG 1 cut(s) 429
BstH2I RGCGCY 1 cut(s) 1998
BstHHI GCGC 3 cut(s) 330, 429, 1997
BstKTI GATC 5 cut(s) 15, 505, 678, 1231, 1554
BstMAI GTCTC 4 cut(s) 641, 677, 995, 2190
BstMBI GATC 5 cut(s) 12, 502, 675, 1228, 1551
BstMCI CGRYCG 1 cut(s) 1891
BstNI CCWGG 3 cut(s) 1034, 1799, 1843
BstNSI RCATGY 1 cut(s) 781
BstSCI CCNGG 4 cut(s) 374, 1032, 1797, 1841
BstSFI CTRYAG 1 cut(s) 1974
BstSLI GKGCMC 1 cut(s) 2105
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 6 cut(s) 577, 706, 1190, 1561, 1603, 2125
BstV2I GAAGAC 2 cut(s) 1556, 2234
BstX2I RGATCY 1 cut(s) 1228
BstXI CCANNNNNNTGG 1 cut(s) 1100
BstYI RGATCY 1 cut(s) 1228
BstZ17I GTATAC 1 cut(s) 2093
BsuRI GGCC 2 cut(s) 5, 388
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 7 cut(s) 252, 496, 826, 1042, 1450, 1600, 2155
BtsI GCAGTG 2 cut(s) 1828, 1983
BtsIMutI CAGTG 3 cut(s) 389, 1828, 1983
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 6 cut(s) 138, 287, 717, 783, 1764, 1776
CaiI CAGNNNCTG 1 cut(s) 915
CciI TCATGA 1 cut(s) 666
CfoI GCGC 3 cut(s) 330, 429, 1997
Cfr10I RCCGGY 1 cut(s) 1890
Cfr13I GGNCC 2 cut(s) 1261, 1845
CsiI ACCWGGT 1 cut(s) 1841
Csp6I GTAC 7 cut(s) 60, 542, 754, 1115, 1659, 2047, 2172
CspAI ACCGGT 1 cut(s) 1890
CviAII CATG 5 cut(s) 7, 418, 667, 778, 1332
CviQI GTAC 7 cut(s) 60, 542, 754, 1115, 1659, 2047, 2172
DpnI GATC 5 cut(s) 14, 504, 677, 1230, 1553
DpnII GATC 5 cut(s) 12, 502, 675, 1228, 1551
DraI TTTAAA 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 1196
DrdI GACNNNNNNGTC 3 cut(s) 304, 691, 1895
DriI GACNNNNNGTC 1 cut(s) 1341
DseDI GACNNNNNNGTC 3 cut(s) 304, 691, 1895
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1427
Eam1105I GACNNNNNGTC 1 cut(s) 1341
EarI CTCTTC 1 cut(s) 1427
Eco130I CCWWGG 4 cut(s) 6, 459, 615, 1547
Eco31I GGTCTC 1 cut(s) 2190
Eco32I GATATC 1 cut(s) 2116
Eco47I GGWCC 2 cut(s) 1261, 1845
Eco47III AGCGCT 1 cut(s) 1996
Eco57I CTGAAG 4 cut(s) 84, 491, 597, 1715
EcoRII CCWGG 3 cut(s) 1032, 1797, 1841
EcoRV GATATC 1 cut(s) 2116
EcoT14I CCWWGG 4 cut(s) 6, 459, 615, 1547
ErhI CCWWGG 4 cut(s) 6, 459, 615, 1547
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 5 cut(s) 10, 421, 670, 781, 1335
FalI AAGNNNNNCTT 6 cut(s) 570, 602, 2116, 2148, 2174, 2206
FaqI GGGAC 2 cut(s) 784, 935
FatI CATG 5 cut(s) 6, 417, 666, 777, 1331
FauNDI CATATG 1 cut(s) 1621
FblI GTMKAC 1 cut(s) 2092
Fnu4HI GCNGC 6 cut(s) 591, 720, 1179, 1575, 1617, 2139
FokI GGATG 7 cut(s) 259, 483, 833, 1049, 1457, 1607, 2162
Fsp4HI GCNGC 6 cut(s) 591, 720, 1179, 1575, 1617, 2139
FspBI CTAG 6 cut(s) 137, 200, 998, 1079, 1548, 1578
GlaI GCGC 3 cut(s) 329, 428, 1996
GluI GCNGC 6 cut(s) 591, 720, 1179, 1575, 1617, 2139
GsuI CTGGAG 2 cut(s) 877, 1781
HaeII RGCGCY 1 cut(s) 1998
HaeIII GGCC 2 cut(s) 5, 388
HapII CCGG 3 cut(s) 376, 932, 1891
HhaI GCGC 3 cut(s) 330, 429, 1997
Hin1II CATG 5 cut(s) 10, 421, 670, 781, 1335
Hin6I GCGC 3 cut(s) 328, 427, 1995
HinP1I GCGC 3 cut(s) 328, 427, 1995
HincII GTYRAC 3 cut(s) 456, 1857, 1969
HindII GTYRAC 3 cut(s) 456, 1857, 1969
HindIII AAGCTT 2 cut(s) 2079, 2130
HpaII CCGG 3 cut(s) 376, 932, 1891
HphI GGTGA 2 cut(s) 1441, 1489
Hpy166II GTNNAC 7 cut(s) 456, 523, 1256, 1268, 1857, 1969, 2093
Hpy8I GTNNAC 7 cut(s) 456, 523, 1256, 1268, 1857, 1969, 2093
HpyAV CCTTC 6 cut(s) 125, 162, 848, 1202, 2104, 2129
HpyCH4III ACNGT 6 cut(s) 384, 453, 572, 917, 1099, 1253
HpyCH4IV ACGT 1 cut(s) 955
HpySE526I ACGT 1 cut(s) 955
Hsp92II CATG 5 cut(s) 10, 421, 670, 781, 1335
HspAI GCGC 3 cut(s) 328, 427, 1995
Kzo9I GATC 5 cut(s) 12, 502, 675, 1228, 1551
LguI GCTCTTC 1 cut(s) 1427
LmnI GCTCC 2 cut(s) 1453, 2002
Lsp1109I GCAGC 6 cut(s) 577, 706, 1190, 1561, 1603, 2125
LweI GCATC 6 cut(s) 37, 672, 1002, 1435, 1977, 2079
MabI ACCWGGT 1 cut(s) 1841
MaeI CTAG 6 cut(s) 137, 200, 998, 1079, 1548, 1578
MaeII ACGT 1 cut(s) 955
MaeIII GTNAC 1 cut(s) 2212
MalI GATC 5 cut(s) 14, 504, 677, 1230, 1553
MboI GATC 5 cut(s) 12, 502, 675, 1228, 1551
MboII GAAGA 7 cut(s) 497, 682, 1031, 1397, 1444, 1556, 2234
MfeI CAATTG 2 cut(s) 741, 846
MflI RGATCY 1 cut(s) 1228
MhlI GDGCHC 2 cut(s) 2014, 2105
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 917, 1330, 1723, 1964, 2015
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 1563, 1953
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 189, 267, 411, 1244, 1292, 1440, 1631
MslI CAYNNNNRTG 1 cut(s) 671
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 539, 905
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 3 cut(s) 376, 932, 1891
MspR9I CCNGG 4 cut(s) 376, 1034, 1799, 1843
MunI CAATTG 2 cut(s) 741, 846
Mva1269I GAATGC 1 cut(s) 1521
MvaI CCWGG 3 cut(s) 1034, 1799, 1843
MvnI CGCG 1 cut(s) 429
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 1621
NdeII GATC 5 cut(s) 12, 502, 675, 1228, 1551
NheI GCTAGC 1 cut(s) 136
NlaIII CATG 5 cut(s) 10, 421, 670, 781, 1335
NlaIV GGNNCC 1 cut(s) 2104
NspI RCATGY 1 cut(s) 781
NspV TTCGAA 1 cut(s) 1601
PagI TCATGA 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 1427
PctI GAATGC 1 cut(s) 1521
PdmI GAANNNNTTC 2 cut(s) 1563, 1953
PfeI GAWTC 8 cut(s) 71, 161, 212, 421, 556, 1126, 1598, 1625
PinAI ACCGGT 1 cut(s) 1890
PkrI GCNGC 6 cut(s) 592, 721, 1180, 1576, 1618, 2140
PleI GAGTC 5 cut(s) 917, 1330, 1722, 1964, 2015
PpsI GAGTC 5 cut(s) 917, 1330, 1722, 1964, 2015
PsiI TTATAA 2 cut(s) 1679, 1805
Psp6I CCWGG 3 cut(s) 1032, 1797, 1841
PspGI CCWGG 3 cut(s) 1032, 1797, 1841
PspN4I GGNNCC 1 cut(s) 2104
PspPI GGNCC 2 cut(s) 1261, 1845
PsrI GAACNNNNNNTAC 2 cut(s) 513, 545
PstI CTGCAG 1 cut(s) 1978
PstNI CAGNNNCTG 1 cut(s) 915
PsuI RGATCY 1 cut(s) 1228
PvuII CAGCTG 2 cut(s) 539, 905
RsaI GTAC 7 cut(s) 61, 543, 755, 1116, 1660, 2048, 2173
RsaNI GTAC 7 cut(s) 60, 542, 754, 1115, 1659, 2047, 2172
RseI CAYNNNNRTG 1 cut(s) 671
SapI GCTCTTC 1 cut(s) 1427
SaqAI TTAA 7 cut(s) 189, 267, 411, 1244, 1292, 1440, 1631
SatI GCNGC 6 cut(s) 591, 720, 1179, 1575, 1617, 2139
Sau3AI GATC 5 cut(s) 12, 502, 675, 1228, 1551
Sau96I GGNCC 2 cut(s) 1261, 1845
SchI GAGTC 5 cut(s) 917, 1330, 1723, 1964, 2015
ScrFI CCNGG 4 cut(s) 376, 1034, 1799, 1843
SduI GDGCHC 2 cut(s) 2014, 2105
SexAI ACCWGGT 1 cut(s) 1841
SfaNI GCATC 6 cut(s) 37, 672, 1002, 1435, 1977, 2079
SfcI CTRYAG 1 cut(s) 1974
SfuI TTCGAA 1 cut(s) 1601
SinI GGWCC 2 cut(s) 1261, 1845
SmiI ATTTAAAT 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 2 cut(s) 188, 2060
SmoI CTYRAG 2 cut(s) 188, 2060
SspI AATATT 1 cut(s) 993
SspMI CTAG 6 cut(s) 137, 200, 998, 1079, 1548, 1578
StyD4I CCNGG 4 cut(s) 374, 1032, 1797, 1841
StyI CCWWGG 4 cut(s) 6, 459, 615, 1547
SwaI ATTTAAAT 1 cut(s) 268
TaaI ACNGT 6 cut(s) 384, 453, 572, 917, 1099, 1253
TaiI ACGT 1 cut(s) 958
TaqI TCGA 2 cut(s) 1601, 1887
TatI WGTACW 2 cut(s) 541, 753
TfiI GAWTC 8 cut(s) 71, 161, 212, 421, 556, 1126, 1598, 1625
Tru1I TTAA 7 cut(s) 189, 267, 411, 1244, 1292, 1440, 1631
Tru9I TTAA 7 cut(s) 189, 267, 411, 1244, 1292, 1440, 1631
TscAI CASTG 3 cut(s) 389, 1828, 1983
TseI GCWGC 6 cut(s) 590, 719, 1178, 1574, 1616, 2138
TspDTI ATGAA 6 cut(s) 162, 569, 683, 1310, 1317, 1638
TspGWI ACGGA 3 cut(s) 572, 665, 1253
TspRI CASTG 3 cut(s) 389, 1828, 1983
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 1261, 1845
XapI RAATTY 3 cut(s) 86, 975, 1355
XceI RCATGY 1 cut(s) 781
XmaJI CCTAGG 1 cut(s) 1547
XmiI GTMKAC 1 cut(s) 2092
XmnI GAANNNNTTC 2 cut(s) 1563, 1953
XspI CTAG 6 cut(s) 137, 200, 998, 1079, 1548, 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.