Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003507

Physical Location & Seq

Genomic Coordinates Forward (+)

25678637 .. 25683919

Gene Structure 5283 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1176600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGGCTGTGGAGATTACTCAGTTTCTATTGGCAGCTCAATCAGCAGATGCAAGGGTTCGTACTGAAGCAGAGGCTAATATTAGGCAATTCCAAGAGCAAAACCTTCCTGCTTTTCTTCTGTCGTTGTCCGTTGAGCTTGCAAACAATGAGAAACCCACTGAATCCCGTACGTTGGCTGGTCTTGTGCTTAAGAATTCATTAGATGCCAAAGATGCTGGTACAAAAGAAATTCTTACTCGACAATGGATGGCTATTGATATTTCTATTGTATCCCAAATTAAGGACTTGCTCTTAAGGACACTTGGGTCATCTGTCCCGGAAGCAAGACACACATCTGCCCAAGTTATTGCAAAAATTGCATCTATTGATATTCCCAGGAAACAATGGCCTGACCTAATCGGGTCTTTGCTCAACAATATGACTCAGCGAGATAGTGCAGCTGGTTTGAAGCAGTCAACCTTGGAAGCACTTGGTTATGTATGTGAGGAGATATCTCATCAAGATCTTGAGCAAGGCGAGGTGAACAATGTTCTTACTGCTGTAGTCCAGGGAATGAACCTTGCTGAAAATAGCCCTGAAGTTCATCTGGCTGCAATAAGGGCTTTATATAATGCCCTGGAGTTCGCACAAACCAACTTTGACACTCAGATGGAACGGGATTTCATCATGAAGATGGTCTGCGAGACGGCCTTGTCTAAGGAGGTTCAAATTAGGCAGGCTGCTTTTGAGTGTCTCGCTTCAATTGCTTCTAGATATTATGAGTTGCTTGGGCCTTACATGCAGGCTCTCTTTGAGCTTACATCAAATGCTGTAAAAGGGGATGAAGAGACAGTTGCCCTTCAAGCAATTGAGTTTTGGAGCTCCATTTGTGATGAAGAAATAGAGCTTCAAGAATTTGAGATCTCTGACACTGGGGACTCTCATTCAAAATTTATAGAAAAAGCCTTGTCATCTTTAGTTCCAATGTTGCTAGAAACTTTGCTGAAACAGGAAGAAAATCAAGACCAAGATGACAACATTTGGAATGTTGCGATGGCTGGTGGGACGTGCCTACAACTTGTTTCAAGGACTGTTGGAGATGCCATTCTGCCCCTTGTGATGCCATTTGTGGAGGCTAACATAGTGAAGCCAGATTGGCACTGTCGTGAGGCAGCTACATTTGCATTTGGCTCGGTACTTGAAGGCCCAAGCATAGAGAAGCTATCTGTTCTGGTCCATTCAGGTTTGGATTTTATGCTTAGGTTAATGAAAGATGAAAATAACCATGTCAAAGACACTACTGCCTGGACTCTTAGTCGTATATTCGAGTTTTTGCACGGTCCTGATGGTACTGTGATTTCTTCTGCTAACCTTCCAAAGGTGGTGGAAATATTGGCGGAAGGCACTAAGGATGCCCCAAATGTTGCAGAGAAGGTTTGTTGGGCCATCTATCACCTTTCTCAGGGGTATGAGGAAACCTGCTCCTCTCTCTTTACTCCGTATGTGCCGGGCATCATTGAATGTCTTATTTCCACTGCTAATCGTACAGATGGTGATGATTCTAGATTGAGGTCTTCTGCTTATGAATCGTTGAATGCTGTTGTGAGGTGTTCTAATATTAAAGAAACATCACAAATTATAGCACAACTACTTGTTGTTATTATGAATAAGTTGGGCCAGACTTTAGAACTTTCGATTGTATCGTCAGATGACCAGGAAAAGCAAGGAGACTTGCAAGCTTCTTTTTGTGGTGTTCTACAGGTTATTATTCAGAAGCTTACCAGTGATGATGAAAGTAAGCGTATTATATTGGAAGCAGCTGATCAGATAATGCTTCTGTTTCTTAGAGTGTTTGCCTGTCGTAGTTCTACAGTACATGAGGAAGCAATGCTTGCTATTGGTGCGCTGGCTTATGCAACTGGGCCTGGGTTTGAAAAGTACCTTCCTGAATTGTACAAGTATTTGGAGATGGGGTTGCAGAATTTTGGGGAGTTTCAAGTATGTGCTATCACAGTTGGGGTGGTTGGTGACATTTTCCGTGCATTGGATGAGAAGGCATTACCGTACTGTGATGGGATTATGAGTCACCTGATGAAGGACTTGTCAAGTGAAGCGCTCCACCGGTCTGTCAAGCCTCCCATATTCTCTGTCTTTGGCGACATAGGTCTTGCAATTGGAGAACATTTTGAGAAGTATGTCCCTTATGCAGTGCAGATGATGCAGGGAGCTTCGGAGCTTTGTGCTCGGATGGACACTAGTGATGAGGAGTTAATGGAATATGGAAACCAGCTAAAGTGTAGCATCTTTGAAGCTTATTCTGGTATACTTCAGGGCTTTAAGGATTCAAAGCCTGAGGTCATGTTGCCATATGCCCAACATCTCCTGCAGTTTATAGAATTGGTTTTAAGGGACGTAAACAGGGATGCCAATGTGACAAAAGCTGCAGTTTCTGCGCTGGGTGACCTTGTTGATGTGCTTGGCCTAACCATCAAGCCACTGTTTGGGGATTTTGCATTCTTTGAGGGAATTTTGCAGGAGTGTCTTCAAACCGATGACGAAAGTCTAAGTGAAACAACAGCTTGGGCTTATGCGATTGTGAGGCGCATTATGAATCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N

Protein Analysis

866

Amino Acids

95.77

Weight (kDa)

4.6

Isoelectric Point (pI)

43.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	23 - 103	5.6e-10	Importin-beta N-terminal domain
TPR_IPO5	PF25780	106 - 266	1.8e-09	IPO5-like, TPR repeats
HEAT_EZ	PF13513	379 - 434	8.4e-10	HEAT-like repeat
TPR_IMB1	PF25574	454 - 819	2.5e-98	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003507)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08947 AT3G08947
fragaria_vesca	FvH4_6g10890 FvH4_6g10890 FvH4_6g10890 FvH4_6g10970
malus_domestica	MD04G1163100.v1.1 MD12G1176600.v1.1
prunus_persica	Prupe.6G282600_v2.0.a1 Prupe.6G282600_v2.0.a1
pyrus_communis	pycom04g14590 pycom04g14600 pycom12g16660 pycom12g16670
rosa_chinensis	RchiOBHm_Chr3g0462121 RchiOBHm_Chr3g0462271
rosa_laevigata	RLG00000024872 RLG00000024881
rosa_multiflora	Rmu_sc0006188.1_g000001 Rmu_sc0007627.1_g000010 Rmu_sc0020622.1_g000002 Rmu_sc0031852.1_g000001
rosa_roxburghii	Rroxscaffold_6G00417630 Rroxscaffold_6G00417720
rosa_rugosa	Rorug03G0055400
rosa_samantha	Rh3AG113300 Rh3AG114100 Rh3BG116200 Rh3BG117200 Rh3CG119000 Rh3CG120000 Rh3DG118100 Rh3DG119000
rosa_wichuraiana	Rw3G009560 Rw3G009630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	304, 691
Acc36I	ACCTGC	1 cut(s)	1466
AccB7I	CCANNNNNTGG	1 cut(s)	2480
AccI	GTMKAC	1 cut(s)	2302
AciI	CCGC	1 cut(s)	1376
AcsI	RAATTY	6 cut(s)	193, 228, 893, 929, 1960, 2505
AcuI	CTGAAG	3 cut(s)	84, 597, 2291
AfeI	AGCGCT	1 cut(s)	2094
AfiI	CCNNNNNNNGG	7 cut(s)	172, 280, 1357, 1441, 2023, 2074, 2480
AflII	CTTAAG	2 cut(s)	188, 292
AgeI	ACCGGT	1 cut(s)	2100
AhdI	GACNNNNNGTC	1 cut(s)	1293
AhlI	ACTAGT	1 cut(s)	2234
AjiI	CACGTC	1 cut(s)	1047
AjnI	CCWGG	6 cut(s)	374, 546, 615, 1283, 1692, 1903
AleI	CACNNNNGTG	1 cut(s)	1143
Alw21I	GWGCWC	2 cut(s)	863, 2224
Alw26I	GTCTC	4 cut(s)	677, 737, 821, 1701
AlwNI	CAGNNNCTG	1 cut(s)	2429
Aor51HI	AGCGCT	1 cut(s)	2094
AoxI	GGCC	8 cut(s)	386, 687, 770, 1183, 1422, 1654, 1901, 2458
ApeKI	GCWGC	7 cut(s)	32, 437, 590, 719, 1151, 1796, 2420
ApoI	RAATTY	6 cut(s)	193, 228, 893, 929, 1960, 2505
AsiGI	ACCGGT	1 cut(s)	2100
AspLEI	GCGC	4 cut(s)	1885, 2095, 2434, 2583
AspS9I	GGNCC	7 cut(s)	770, 1184, 1213, 1319, 1422, 1654, 1901
AsuC2I	CCSGG	2 cut(s)	317, 1488
AsuHPI	GGTGA	6 cut(s)	532, 1424, 1544, 2018, 2057, 2450
AvaII	GGWCC	2 cut(s)	1213, 1319
AxyI	CCTNAGG	1 cut(s)	2331
BanII	GRGCYC	1 cut(s)	863
BbsI	GAAGAC	2 cut(s)	1545, 2513
Bbv12I	GWGCWC	2 cut(s)	863, 2224
BbvI	GCAGC	7 cut(s)	44, 449, 577, 706, 1163, 1808, 2407
BceAI	ACGGC	1 cut(s)	702
BcgI	CGANNNNNNTGC	2 cut(s)	1152, 1186
BciT130I	CCWGG	6 cut(s)	376, 548, 617, 1285, 1694, 1905
BciVI	GTATCC	1 cut(s)	280
BclI	TGATCA	1 cut(s)	1801
BcnI	CCSGG	2 cut(s)	317, 1488
BcoDI	GTCTC	4 cut(s)	677, 737, 821, 1701
BcuI	ACTAGT	1 cut(s)	2234
BfaI	CTAG	4 cut(s)	750, 971, 1542, 2235
BfmI	CTRYAG	5 cut(s)	540, 1736, 1848, 2363, 2421
BfoI	RGCGCY	1 cut(s)	2096
BfrI	CTTAAG	2 cut(s)	188, 292
BfuAI	ACCTGC	1 cut(s)	1466
BfuI	GTATCC	1 cut(s)	280
BglI	GCCNNNNNGGC	1 cut(s)	1135
BglII	AGATCT	2 cut(s)	502, 900
BisI	GCNGC	7 cut(s)	33, 438, 591, 720, 1152, 1797, 2421
BlsI	GCNGC	7 cut(s)	34, 439, 592, 721, 1153, 1798, 2422
Bme1390I	CCNGG	8 cut(s)	317, 376, 548, 617, 1285, 1488, 1694, 1905
Bme18I	GGWCC	2 cut(s)	1213, 1319
BmeRI	GACNNNNNGTC	1 cut(s)	1293
BmgBI	CACGTC	1 cut(s)	1047
BmgT120I	GGNCC	7 cut(s)	770, 1184, 1213, 1319, 1422, 1654, 1901
BmrFI	CCNGG	8 cut(s)	317, 376, 548, 617, 1285, 1488, 1694, 1905
BmrI	ACTGGG	2 cut(s)	921, 1908
BmuI	ACTGGG	2 cut(s)	921, 1908
BoxI	GACNNNNGTC	2 cut(s)	2142, 2538
BpiI	GAAGAC	2 cut(s)	1545, 2513
BpmI	CTGGAG	1 cut(s)	638
Bpu10I	CCTNAGC	1 cut(s)	1238
BpuEI	CTTGAG	1 cut(s)	527
BpuMI	CCSGG	2 cut(s)	317, 1488
BsaJI	CCNNGG	5 cut(s)	374, 459, 547, 615, 1904
BsaWI	WCCGGW	1 cut(s)	2100
BsaXI	ACNNNNNCTCC	2 cut(s)	1937, 1967
Bsc4I	CCNNNNNNNGG	7 cut(s)	172, 280, 1357, 1441, 2023, 2074, 2480
Bse118I	RCCGGY	1 cut(s)	2100
Bse1I	ACTGG	3 cut(s)	916, 1761, 1903
Bse21I	CCTNAGG	1 cut(s)	2331
Bse3DI	GCAATG	1 cut(s)	1872
BseBI	CCWGG	6 cut(s)	376, 548, 617, 1285, 1694, 1905
BseDI	CCNNGG	5 cut(s)	374, 459, 547, 615, 1904
BseGI	GGATG	6 cut(s)	252, 826, 1396, 2032, 2232, 2407
BseLI	CCNNNNNNNGG	7 cut(s)	172, 280, 1357, 1441, 2023, 2074, 2480
BseMI	GCAATG	1 cut(s)	1872
BseMII	CTCAG	5 cut(s)	32, 437, 659, 1454, 2322
BseNI	ACTGG	3 cut(s)	916, 1761, 1903
BseRI	GAGGAG	3 cut(s)	500, 1453, 2258
BseXI	GCAGC	7 cut(s)	44, 449, 577, 706, 1163, 1808, 2407
BseYI	CCCAGC	1 cut(s)	2434
BsgI	GTGCAG	2 cut(s)	456, 2210
BshFI	GGCC	8 cut(s)	388, 689, 772, 1185, 1424, 1656, 1903, 2460
BshTI	ACCGGT	1 cut(s)	2100
BsiHKAI	GWGCWC	2 cut(s)	863, 2224
BsiSI	CCGG	3 cut(s)	317, 1487, 2101
BsiWI	CGTACG	1 cut(s)	167
BslFI	GGGAC	5 cut(s)	299, 929, 1057, 2162, 2402
BslI	CCNNNNNNNGG	7 cut(s)	172, 280, 1357, 1441, 2023, 2074, 2480
BsmAI	GTCTC	4 cut(s)	677, 737, 821, 1701
BsmBI	CGTCTC	1 cut(s)	677
BsmFI	GGGAC	5 cut(s)	299, 929, 1057, 2162, 2402
BsmI	GAATGC	2 cut(s)	1579, 2492
BsnI	GGCC	8 cut(s)	388, 689, 772, 1185, 1424, 1656, 1903, 2460
Bsp1286I	GDGCHC	2 cut(s)	863, 2224
Bsp1407I	TGTACA	1 cut(s)	1932
Bsp143I	GATC	3 cut(s)	502, 900, 1801
BspACI	CCGC	1 cut(s)	1376
BspANI	GGCC	8 cut(s)	388, 689, 772, 1185, 1424, 1656, 1903, 2460
BspCNI	CTCAG	5 cut(s)	31, 436, 658, 1453, 2323
BspHI	TCATGA	1 cut(s)	666
BspMAI	CTGCAG	2 cut(s)	2367, 2425
BspMI	ACCTGC	1 cut(s)	1466
BspTI	CTTAAG	2 cut(s)	188, 292
BsrDI	GCAATG	1 cut(s)	1872
BsrFI	RCCGGY	1 cut(s)	2100
BsrGI	TGTACA	1 cut(s)	1932
BsrI	ACTGG	3 cut(s)	916, 1761, 1903
BssAI	RCCGGY	1 cut(s)	2100
BssECI	CCNNGG	5 cut(s)	374, 459, 547, 615, 1904
BssMI	GATC	3 cut(s)	502, 900, 1801
BssNAI	GTATAC	1 cut(s)	2303
BssT1I	CCWWGG	1 cut(s)	459
Bst1107I	GTATAC	1 cut(s)	2303
Bst2UI	CCWGG	6 cut(s)	376, 548, 617, 1285, 1694, 1905
Bst6I	CTCTTC	1 cut(s)	819
BstAFI	CTTAAG	2 cut(s)	188, 292
BstAPI	GCANNNNNTGC	4 cut(s)	356, 1871, 2197, 2429
BstAUI	TGTACA	1 cut(s)	1932
BstC8I	GCNNGC	6 cut(s)	138, 717, 783, 1716, 1872, 1887
BstEII	GGTNACC	1 cut(s)	2438
BstENI	CCTNNNNNAGG	3 cut(s)	1355, 1439, 2072
BstF5I	GGATG	6 cut(s)	252, 826, 1396, 2032, 2232, 2407
BstH2I	RGCGCY	1 cut(s)	2096
BstHHI	GCGC	4 cut(s)	1885, 2095, 2434, 2583
BstKTI	GATC	3 cut(s)	505, 903, 1804
BstMAI	GTCTC	4 cut(s)	677, 737, 821, 1701
BstMBI	GATC	3 cut(s)	502, 900, 1801
BstNI	CCWGG	6 cut(s)	376, 548, 617, 1285, 1694, 1905
BstNSI	RCATGY	1 cut(s)	781
BstPAI	GACNNNNGTC	2 cut(s)	2142, 2538
BstPI	GGTNACC	1 cut(s)	2438
BstSCI	CCNGG	8 cut(s)	315, 374, 546, 615, 1283, 1486, 1692, 1903
BstSFI	CTRYAG	5 cut(s)	540, 1736, 1848, 2363, 2421
BstV1I	GCAGC	7 cut(s)	44, 449, 577, 706, 1163, 1808, 2407
BstV2I	GAAGAC	2 cut(s)	1545, 2513
BstX2I	RGATCY	2 cut(s)	502, 900
BstYI	RGATCY	2 cut(s)	502, 900
BstZ17I	GTATAC	1 cut(s)	2303
Bsu36I	CCTNAGG	1 cut(s)	2331
BsuI	GTATCC	1 cut(s)	280
BsuRI	GGCC	8 cut(s)	388, 689, 772, 1185, 1424, 1656, 1903, 2460
BtgZI	GCGATG	1 cut(s)	1046
BtrI	CACGTC	1 cut(s)	1047
BtsCI	GGATG	6 cut(s)	252, 826, 1396, 2032, 2232, 2407
BtsI	GCAGTG	2 cut(s)	1512, 2193
BtsIMutI	CAGTG	7 cut(s)	156, 909, 1138, 1512, 1768, 2193, 2474
BveI	ACCTGC	1 cut(s)	1466
Cac8I	GCNNGC	6 cut(s)	138, 717, 783, 1716, 1872, 1887
CaiI	CAGNNNCTG	1 cut(s)	2429
CciI	TCATGA	1 cut(s)	666
CfoI	GCGC	4 cut(s)	1885, 2095, 2434, 2583
Cfr10I	RCCGGY	1 cut(s)	2100
Cfr13I	GGNCC	7 cut(s)	770, 1184, 1213, 1319, 1422, 1654, 1901
CspAI	ACCGGT	1 cut(s)	2100
CspCI	CAANNNNNGTGG	2 cut(s)	1344, 1379
CviAII	CATG	5 cut(s)	667, 778, 1265, 1856, 2338
DpnI	GATC	3 cut(s)	504, 902, 1803
DpnII	GATC	3 cut(s)	502, 900, 1801
DrdI	GACNNNNNNGTC	2 cut(s)	304, 691
DriI	GACNNNNNGTC	1 cut(s)	1293
DseDI	GACNNNNNNGTC	2 cut(s)	304, 691
Eam1104I	CTCTTC	1 cut(s)	819
Eam1105I	GACNNNNNGTC	1 cut(s)	1293
EarI	CTCTTC	1 cut(s)	819
EciI	GGCGGA	1 cut(s)	1391
Ecl136II	GAGCTC	1 cut(s)	861
Eco130I	CCWWGG	1 cut(s)	459
Eco24I	GRGCYC	1 cut(s)	863
Eco32I	GATATC	1 cut(s)	492
Eco47I	GGWCC	2 cut(s)	1213, 1319
Eco47III	AGCGCT	1 cut(s)	2094
Eco53kI	GAGCTC	1 cut(s)	861
Eco57I	CTGAAG	3 cut(s)	84, 597, 2291
Eco81I	CCTNAGG	1 cut(s)	2331
Eco91I	GGTNACC	1 cut(s)	2438
EcoICRI	GAGCTC	1 cut(s)	861
EcoNI	CCTNNNNNAGG	3 cut(s)	1355, 1439, 2072
EcoO65I	GGTNACC	1 cut(s)	2438
EcoRI	GAATTC	1 cut(s)	193
EcoRII	CCWGG	6 cut(s)	374, 546, 615, 1283, 1692, 1903
EcoRV	GATATC	1 cut(s)	492
EcoT14I	CCWWGG	1 cut(s)	459
EcoT38I	GRGCYC	1 cut(s)	863
ErhI	CCWWGG	1 cut(s)	459
Esp3I	CGTCTC	1 cut(s)	677
FaeI	CATG	5 cut(s)	670, 781, 1268, 1859, 2341
FalI	AAGNNNNNCTT	4 cut(s)	216, 248, 1854, 1886
FaqI	GGGAC	5 cut(s)	299, 929, 1057, 2162, 2402
FatI	CATG	5 cut(s)	666, 777, 1264, 1855, 2337
FauNDI	CATATG	1 cut(s)	2347
FbaI	TGATCA	1 cut(s)	1801
FblI	GTMKAC	1 cut(s)	2302
Fnu4HI	GCNGC	7 cut(s)	33, 438, 591, 720, 1152, 1797, 2421
FokI	GGATG	6 cut(s)	259, 833, 1403, 2039, 2239, 2414
FriOI	GRGCYC	1 cut(s)	863
Fsp4HI	GCNGC	7 cut(s)	33, 438, 591, 720, 1152, 1797, 2421
FspBI	CTAG	4 cut(s)	750, 971, 1542, 2235
GlaI	GCGC	4 cut(s)	1884, 2094, 2433, 2582
GluI	GCNGC	7 cut(s)	33, 438, 591, 720, 1152, 1797, 2421
GsaI	CCCAGC	1 cut(s)	2438
GsuI	CTGGAG	1 cut(s)	638
HaeII	RGCGCY	1 cut(s)	2096
HaeIII	GGCC	8 cut(s)	388, 689, 772, 1185, 1424, 1656, 1903, 2460
HapII	CCGG	3 cut(s)	317, 1487, 2101
HhaI	GCGC	4 cut(s)	1885, 2095, 2434, 2583
Hin1II	CATG	5 cut(s)	670, 781, 1268, 1859, 2341
Hin6I	GCGC	4 cut(s)	1883, 2093, 2432, 2581
HinP1I	GCGC	4 cut(s)	1883, 2093, 2432, 2581
HincII	GTYRAC	1 cut(s)	456
HindII	GTYRAC	1 cut(s)	456
HindIII	AAGCTT	3 cut(s)	1716, 1754, 2289
HinfI	GANTC	9 cut(s)	161, 421, 917, 1288, 1538, 1565, 2062, 2321, 2590
HpaII	CCGG	3 cut(s)	317, 1487, 2101
HphI	GGTGA	6 cut(s)	532, 1424, 1544, 2018, 2057, 2450
Hpy166II	GTNNAC	4 cut(s)	456, 523, 2303, 2395
Hpy188I	TCNGA	7 cut(s)	648, 907, 1687, 1752, 1806, 2212, 2226
Hpy8I	GTNNAC	4 cut(s)	456, 523, 2303, 2395
HpyAV	CCTTC	9 cut(s)	113, 848, 1175, 1361, 1373, 1405, 1931, 2026, 2068
HpyCH4IV	ACGT	3 cut(s)	170, 1046, 2391
HpySE526I	ACGT	3 cut(s)	170, 1046, 2391
Hsp92II	CATG	5 cut(s)	670, 781, 1268, 1859, 2341
HspAI	GCGC	4 cut(s)	1883, 2093, 2432, 2581
Ksp22I	TGATCA	1 cut(s)	1801
Kzo9I	GATC	3 cut(s)	502, 900, 1801
LmnI	GCTCC	6 cut(s)	858, 866, 1466, 2100, 2204, 2212
Lsp1109I	GCAGC	7 cut(s)	44, 449, 577, 706, 1163, 1808, 2407
MaeI	CTAG	4 cut(s)	750, 971, 1542, 2235
MaeII	ACGT	3 cut(s)	170, 1046, 2391
MaeIII	GTNAC	4 cut(s)	2006, 2063, 2410, 2438
MalI	GATC	3 cut(s)	504, 902, 1803
MboI	GATC	3 cut(s)	502, 900, 1801
MboII	GAAGA	8 cut(s)	107, 682, 836, 887, 1004, 1332, 1545, 2513
MfeI	CAATTG	3 cut(s)	741, 846, 2151
MflI	RGATCY	2 cut(s)	502, 900
MhlI	GDGCHC	2 cut(s)	863, 2224
MlyI	GAGTC	4 cut(s)	415, 911, 1282, 2071
MmeI	TCCRAC	1 cut(s)	1054
MseI	TTAA	8 cut(s)	189, 279, 293, 1244, 1599, 2249, 2316, 2384
MslI	CAYNNNNRTG	3 cut(s)	647, 671, 1143
MspA1I	CMGCKG	2 cut(s)	440, 1799
MspCI	CTTAAG	2 cut(s)	188, 292
MspI	CCGG	3 cut(s)	317, 1487, 2101
MspR9I	CCNGG	8 cut(s)	317, 376, 548, 617, 1285, 1488, 1694, 1905
MunI	CAATTG	3 cut(s)	741, 846, 2151
Mva1269I	GAATGC	2 cut(s)	1579, 2492
MvaI	CCWGG	6 cut(s)	376, 548, 617, 1285, 1694, 1905
NciI	CCSGG	2 cut(s)	317, 1488
NdeI	CATATG	1 cut(s)	2347
NdeII	GATC	3 cut(s)	502, 900, 1801
NlaIII	CATG	5 cut(s)	670, 781, 1268, 1859, 2341
NmuCI	GTSAC	4 cut(s)	2006, 2063, 2410, 2438
NspI	RCATGY	1 cut(s)	781
OliI	CACNNNNGTG	1 cut(s)	1143
PagI	TCATGA	1 cut(s)	666
PcsI	WCGNNNNNNNCGW	1 cut(s)	1679
PctI	GAATGC	2 cut(s)	1579, 2492
PfeI	GAWTC	5 cut(s)	161, 1538, 1565, 2321, 2590
Pfl23II	CGTACG	1 cut(s)	167
PflMI	CCANNNNNTGG	1 cut(s)	2480
PfoI	TCCNGGA	1 cut(s)	315
PinAI	ACCGGT	1 cut(s)	2100
PkrI	GCNGC	7 cut(s)	34, 439, 592, 721, 1153, 1798, 2422
PleI	GAGTC	4 cut(s)	415, 911, 1282, 2070
PpsI	GAGTC	4 cut(s)	415, 911, 1282, 2070
PshAI	GACNNNNGTC	2 cut(s)	2142, 2538
Psp124BI	GAGCTC	1 cut(s)	863
Psp6I	CCWGG	6 cut(s)	374, 546, 615, 1283, 1692, 1903
PspEI	GGTNACC	1 cut(s)	2438
PspFI	CCCAGC	1 cut(s)	2434
PspGI	CCWGG	6 cut(s)	374, 546, 615, 1283, 1692, 1903
PspLI	CGTACG	1 cut(s)	167
PspPI	GGNCC	7 cut(s)	770, 1184, 1213, 1319, 1422, 1654, 1901
PstI	CTGCAG	2 cut(s)	2367, 2425
PstNI	CAGNNNCTG	1 cut(s)	2429
PsuI	RGATCY	2 cut(s)	502, 900
PvuII	CAGCTG	2 cut(s)	440, 1799
RseI	CAYNNNNRTG	3 cut(s)	647, 671, 1143
SacI	GAGCTC	1 cut(s)	863
SaqAI	TTAA	8 cut(s)	189, 279, 293, 1244, 1599, 2249, 2316, 2384
SatI	GCNGC	7 cut(s)	33, 438, 591, 720, 1152, 1797, 2421
Sau3AI	GATC	3 cut(s)	502, 900, 1801
Sau96I	GGNCC	7 cut(s)	770, 1184, 1213, 1319, 1422, 1654, 1901
SchI	GAGTC	4 cut(s)	415, 911, 1282, 2071
ScrFI	CCNGG	8 cut(s)	317, 376, 548, 617, 1285, 1488, 1694, 1905
SduI	GDGCHC	2 cut(s)	863, 2224
SfcI	CTRYAG	5 cut(s)	540, 1736, 1848, 2363, 2421
SinI	GGWCC	2 cut(s)	1213, 1319
SmiMI	CAYNNNNRTG	3 cut(s)	647, 671, 1143
SmlI	CTYRAG	3 cut(s)	188, 292, 506
SmoI	CTYRAG	3 cut(s)	188, 292, 506
SpeI	ACTAGT	1 cut(s)	2234
SsiI	CCGC	1 cut(s)	1376
SspI	AATATT	3 cut(s)	79, 1371, 1597
SspMI	CTAG	4 cut(s)	750, 971, 1542, 2235
SstI	GAGCTC	1 cut(s)	863
StyD4I	CCNGG	8 cut(s)	315, 374, 546, 615, 1283, 1486, 1692, 1903
StyI	CCWWGG	1 cut(s)	459
TaiI	ACGT	3 cut(s)	173, 1049, 2394
TaqI	TCGA	3 cut(s)	238, 1305, 1673
TatI	WGTACW	2 cut(s)	1852, 1932
TfiI	GAWTC	5 cut(s)	161, 1538, 1565, 2321, 2590
Tru1I	TTAA	8 cut(s)	189, 279, 293, 1244, 1599, 2249, 2316, 2384
Tru9I	TTAA	8 cut(s)	189, 279, 293, 1244, 1599, 2249, 2316, 2384
TscAI	CASTG	7 cut(s)	163, 916, 1145, 1519, 1768, 2193, 2481
TseFI	GTSAC	4 cut(s)	2006, 2063, 2410, 2438
TseI	GCWGC	7 cut(s)	32, 437, 590, 719, 1151, 1796, 2420
Tsp45I	GTSAC	4 cut(s)	2006, 2063, 2410, 2438
TspGWI	ACGGA	3 cut(s)	118, 1467, 2006
TspRI	CASTG	7 cut(s)	163, 916, 1145, 1519, 1768, 2193, 2481
Van91I	CCANNNNNTGG	1 cut(s)	2480
Vha464I	CTTAAG	2 cut(s)	188, 292
VpaK11BI	GGWCC	2 cut(s)	1213, 1319
XagI	CCTNNNNNAGG	3 cut(s)	1355, 1439, 2072
XapI	RAATTY	6 cut(s)	193, 228, 893, 929, 1960, 2505
XbaI	TCTAGA	2 cut(s)	749, 1541
XceI	RCATGY	1 cut(s)	781
XmiI	GTMKAC	1 cut(s)	2302
XspI	CTAG	4 cut(s)	750, 971, 1542, 2235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.