MD04G1188600.v1.1

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005202
Physical Location & Seq
Reverse (-)
27920145 .. 27923887
3743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1188600.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGGCAGCAAAGTTGTTTTAAAACCAGTAACTTGTCCTCCATATCTTCTCCTACTACAAAGATGTTCAAGCTTGGACGAATTTAAGAAAATTCATGCCCATGCAGTCACTACTGGTCTTGCTCGTTTTGCCTACACTGCCAGCAAGCTCATAGCCTTCTCTGCATTATCAGAACATGGTAATATGCACTACGCTGAGACACTGTTACACCAAATTCCAAGACCCAATGTATTTGATTACAATTCCATGATAATGGGTTTCTTGAAAAGCTCAGAATCTGAAAAGGGTCTCTCCATTTACCCCAGAATGAGAAGGATAGGCACAGAGCCAAACGCTCGTACTTTCACCTTTTTAGTTAAAGCATGTTTTGATGTATCTTTGCTTGGTCAGGTACATGGTCAAATAATGAAGTTTGGGCATGGGTGTGATGTTTATGTTATTAGCTCACTTATTAGGGCCTCCTGTCGATGTGAAGCTGTGGAATTTGCTCGTAGAGTGTTTGAGGAAAGTTTGGATAAGAATGTGGTTTGTTGGACTAGTCTTATTAGTGGATATTGTAGTAATGGGCTGGTTCATGAAGCTCGTGATCTGTTTGATGCAATGCCAGATATAAATGATGTTTCTTATAGTGCAATGGTTTCTGGGTATGTTGGGAATGCTTGTTTCAATGAAGCAATTGACCTGTTTCGTCAATTGAAAAGCCATACACAAGTGAGGCTCAAAGAGTCTCTTTTGGTTAGCACTCTTAATGCTTGTGCGTCTGTGGGTGCCTTTGAAGAAGGGAAGTGGATTCATTCGTTTTTGGAAGAAAATGGTTTTGAGTATGGACTTGAGCTTGGTACTGCACTAGTAGATTTTTATGCAAAATGTGGATGTATTAAAGACGCACAAGACGTGTTTAGCAGGATGCCTCGTAAAGATGTAACTACTTGGAGTGCTATGATCATGGGATTTGCCATCAATGGTGAGAATGATATGGGGCTTGAGCTTTTTGCAGAAATGGAAAAGAAAGGACCTAAACCAAATGCAGTGACATTTGTTGGAGTACTCACTGCATGTAATCACAAAATTCTGGTAAATGAAGCATGGCGGTTAATAGGACGCATGAGTAAGGTTTATGGCATTGCTCTGGGGATTGAGCACTATGGCTGCATGGTTGATCTCTTGGCCTGTGCTGGGAAACTGAATGAAGCTGAGATATTGATAAGAAGTATGGCAATGAAACCGGACGGTGCTATTTGGGGGTCTTTGTTTAATGGATGTCTGATACATGACCATGTTGACTTAGGGGCGAGGGTGGGGAAGCTTTTGATTGAATTAGAGCCTCAACATAGTGGTAGGTATGTTCTTCTGGCCAACATGTATGCCAAAATGGGCAACTGGGAGGATGTGACGAGGCTAAGGAAAATGATGAGAGATAGAGAAGTAGTTACAATTTCTGCTTGGAGCTTCATTGAGACTGATGGTATTGTCCATAAATTTATAGCAGACGATAAGTCTCATTCCCATTCGAGAAAGATAAACAGAACCTTAAACCAACTCAGCAGGGCACTTGAAAGTTTTTCCATTGCCGGTGACGCATCGTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

531

Amino Acids

59.05

Weight (kDa)

7.0

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 123 8.3e-09 PPR repeat family
PPR_2 PF13041 173 - 202 7.4e-07 PPR repeat family
PPR_1 PF12854 174 - 201 2.2e-09 PPR repeat
PPR PF01535 176 - 202 5.3e-08 PPR repeat
PPR_2 PF13041 306 - 353 9.1e-10 PPR repeat family
PPR PF01535 310 - 338 7.1e-06 PPR repeat
E_motif PF20431 424 - 476 6.6e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 767
AciI CCGC 1 cut(s) 1090
AcoI YGGCCR 1 cut(s) 1353
AcsI RAATTY 6 cut(s) 80, 90, 213, 482, 1068, 1478
AfaI GTAC 4 cut(s) 340, 393, 841, 1047
AfiI CCNNNNNNNGG 1 cut(s) 1176
AflIII ACRYGT 2 cut(s) 894, 1359
AgsI TTSAA 7 cut(s) 69, 265, 667, 697, 776, 1316, 1556
AhdI GACNNNNNGTC 1 cut(s) 1495
AhlI ACTAGT 2 cut(s) 536, 847
AjiI CACGTC 1 cut(s) 895
Alw21I GWGCWC 1 cut(s) 1143
Alw26I GTCTC 5 cut(s) 191, 293, 732, 1451, 1503
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 3 cut(s) 456, 1167, 1353
ApeKI GCWGC 2 cut(s) 6, 1149
ApoI RAATTY 6 cut(s) 80, 90, 213, 482, 1068, 1478
AspS9I GGNCC 2 cut(s) 456, 1013
AsuHPI GGTGA 3 cut(s) 337, 977, 1586
AvaII GGWCC 1 cut(s) 1013
BaeGI GKGCMC 1 cut(s) 1552
BalI TGGCCA 1 cut(s) 1355
BanI GGYRCC 1 cut(s) 767
BauI CACGAG 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 1143
BbvI GCAGC 2 cut(s) 18, 1136
BccI CCATC 2 cut(s) 965, 1457
BclI TGATCA 1 cut(s) 942
BcoDI GTCTC 5 cut(s) 191, 293, 732, 1451, 1503
BcuI ACTAGT 2 cut(s) 536, 847
BfaI CTAG 2 cut(s) 537, 848
BisI GCNGC 2 cut(s) 7, 1150
BlsI GCNGC 2 cut(s) 8, 1151
BmcAI AGTACT 1 cut(s) 1047
Bme18I GGWCC 1 cut(s) 1013
BmeRI GACNNNNNGTC 1 cut(s) 1495
BmgBI CACGTC 1 cut(s) 895
BmgT120I GGNCC 2 cut(s) 456, 1013
BmiI GGNNCC 1 cut(s) 769
BmrI ACTGGG 1 cut(s) 1390
BmsI GCATC 3 cut(s) 586, 897, 1589
BmuI ACTGGG 1 cut(s) 1390
Bpu10I CCTNAGC 1 cut(s) 1400
BpuEI CTTGAG 2 cut(s) 851, 1004
BsaI GGTCTC 1 cut(s) 293
BsaWI WCCGGW 1 cut(s) 1225
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 1176
Bse118I RCCGGY 1 cut(s) 1571
Bse1I ACTGG 3 cut(s) 26, 118, 1385
Bse3DI GCAATG 5 cut(s) 606, 639, 1122, 1224, 1566
BseGI GGATG 4 cut(s) 878, 912, 1265, 1393
BseLI CCNNNNNNNGG 1 cut(s) 1176
BseMI GCAATG 5 cut(s) 606, 639, 1122, 1224, 1566
BseMII CTCAG 4 cut(s) 186, 285, 1185, 1555
BseNI ACTGG 3 cut(s) 26, 118, 1385
BseSI GKGCMC 1 cut(s) 1552
BseXI GCAGC 2 cut(s) 18, 1136
BseYI CCCAGC 1 cut(s) 1175
BsgI GTGCAG 1 cut(s) 828
BshFI GGCC 3 cut(s) 458, 1169, 1355
BshNI GGYRCC 1 cut(s) 767
BsiHKAI GWGCWC 1 cut(s) 1143
BsiSI CCGG 2 cut(s) 1226, 1572
BslI CCNNNNNNNGG 1 cut(s) 1176
BsmAI GTCTC 5 cut(s) 191, 293, 732, 1451, 1503
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 3 cut(s) 458, 1169, 1355
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 1143, 1552
Bsp143I GATC 3 cut(s) 586, 942, 1159
BspACI CCGC 1 cut(s) 1090
BspANI GGCC 3 cut(s) 458, 1169, 1355
BspCNI CTCAG 4 cut(s) 187, 284, 1186, 1554
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 769
BspT107I GGYRCC 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 293
BsrDI GCAATG 5 cut(s) 606, 639, 1122, 1224, 1566
BsrFI RCCGGY 1 cut(s) 1571
BsrI ACTGG 3 cut(s) 26, 118, 1385
BssAI RCCGGY 1 cut(s) 1571
BssMI GATC 3 cut(s) 586, 942, 1159
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 204, 1232
BstC8I GCNNGC 2 cut(s) 142, 146
BstDEI CTNAG 6 cut(s) 195, 271, 1194, 1285, 1400, 1541
BstF5I GGATG 4 cut(s) 878, 912, 1265, 1393
BstKTI GATC 3 cut(s) 589, 945, 1162
BstMAI GTCTC 5 cut(s) 191, 293, 732, 1451, 1503
BstMBI GATC 3 cut(s) 586, 942, 1159
BstMWI GCNNNNNNNGC 4 cut(s) 128, 137, 161, 1577
BstNSI RCATGY 3 cut(s) 366, 1059, 1363
BstSLI GKGCMC 1 cut(s) 1552
BstV1I GCAGC 2 cut(s) 18, 1136
BstXI CCANNNNNNTGG 1 cut(s) 253
BsuRI GGCC 3 cut(s) 458, 1169, 1355
BtrI CACGTC 1 cut(s) 895
BtsCI GGATG 4 cut(s) 878, 912, 1265, 1393
BtsI GCAGTG 3 cut(s) 135, 1035, 1050
BtsIMutI CAGTG 4 cut(s) 135, 200, 1035, 1050
Cac8I GCNNGC 2 cut(s) 142, 146
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 1571
Cfr13I GGNCC 2 cut(s) 456, 1013
CseI GACGC 4 cut(s) 747, 893, 1110, 1586
Csp6I GTAC 4 cut(s) 339, 392, 840, 1046
CviQI GTAC 4 cut(s) 339, 392, 840, 1046
DdeI CTNAG 6 cut(s) 195, 271, 1194, 1285, 1400, 1541
DpnI GATC 3 cut(s) 588, 944, 1161
DpnII GATC 3 cut(s) 586, 942, 1159
DraI TTTAAA 1 cut(s) 21
DriI GACNNNNNGTC 1 cut(s) 1495
EaeI YGGCCR 1 cut(s) 1353
Eam1105I GACNNNNNGTC 1 cut(s) 1495
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 1013
EcoO109I RGGNCCY 2 cut(s) 456, 1013
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FbaI TGATCA 1 cut(s) 942
Fnu4HI GCNGC 2 cut(s) 7, 1150
FokI GGATG 4 cut(s) 885, 919, 1272, 1400
Fsp4HI GCNGC 2 cut(s) 7, 1150
FspBI CTAG 2 cut(s) 537, 848
GluI GCNGC 2 cut(s) 7, 1150
GsaI CCCAGC 1 cut(s) 1179
HaeIII GGCC 3 cut(s) 458, 1169, 1355
HapII CCGG 2 cut(s) 1226, 1572
HgaI GACGC 4 cut(s) 747, 893, 1110, 1586
HincII GTYRAC 1 cut(s) 1282
HindII GTYRAC 1 cut(s) 1282
HindIII AAGCTT 2 cut(s) 70, 1304
HinfI GANTC 3 cut(s) 275, 725, 790
HpaII CCGG 2 cut(s) 1226, 1572
HphI GGTGA 3 cut(s) 337, 977, 1586
Hpy166II GTNNAC 1 cut(s) 1282
Hpy188I TCNGA 4 cut(s) 172, 274, 280, 1266
Hpy188III TCNNGA 4 cut(s) 262, 575, 584, 1512
Hpy8I GTNNAC 1 cut(s) 1282
HpyAV CCTTC 3 cut(s) 166, 306, 773
HpyCH4III ACNGT 2 cut(s) 204, 1232
HpyCH4IV ACGT 1 cut(s) 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 137, 161, 1577
HpyF3I CTNAG 6 cut(s) 195, 271, 1194, 1285, 1400, 1541
HpySE526I ACGT 1 cut(s) 894
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 3 cut(s) 586, 942, 1159
LmnI GCTCC 1 cut(s) 1446
Lsp1109I GCAGC 2 cut(s) 18, 1136
LweI GCATC 3 cut(s) 586, 897, 1589
MaeI CTAG 2 cut(s) 537, 848
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 9 cut(s) 28, 106, 204, 922, 1030, 1390, 1429, 1574, 1584
MalI GATC 3 cut(s) 588, 944, 1161
MboI GATC 3 cut(s) 586, 942, 1159
MboII GAAGA 4 cut(s) 38, 788, 818, 1340
MfeI CAATTG 2 cut(s) 675, 692
MhlI GDGCHC 2 cut(s) 1143, 1552
MlsI TGGCCA 1 cut(s) 1355
MluNI TGGCCA 1 cut(s) 1355
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 512, 1021
MnlI CCTC 9 cut(s) 48, 469, 496, 708, 921, 1287, 1335, 1378, 1389
Mox20I TGGCCA 1 cut(s) 1355
MscI TGGCCA 1 cut(s) 1355
MseI TTAA 9 cut(s) 20, 84, 357, 747, 879, 1094, 1254, 1532, 1591
MslI CAYNNNNRTG 4 cut(s) 99, 251, 423, 1275
Msp20I TGGCCA 1 cut(s) 1355
MspI CCGG 2 cut(s) 1226, 1572
MunI CAATTG 2 cut(s) 675, 692
Mva1269I GAATGC 1 cut(s) 661
MwoI GCNNNNNNNGC 4 cut(s) 128, 137, 161, 1577
NdeII GATC 3 cut(s) 586, 942, 1159
NlaIV GGNNCC 1 cut(s) 769
NmuCI GTSAC 4 cut(s) 106, 1030, 1390, 1574
NspI RCATGY 3 cut(s) 366, 1059, 1363
PagI TCATGA 1 cut(s) 574
PciI ACATGT 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 1 cut(s) 891
PctI GAATGC 1 cut(s) 661
PfeI GAWTC 2 cut(s) 275, 790
PkrI GCNGC 2 cut(s) 8, 1151
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 1013
PscI ACATGT 1 cut(s) 1359
Psp5II RGGWCCY 1 cut(s) 1013
PspFI CCCAGC 1 cut(s) 1175
PspN4I GGNNCC 1 cut(s) 769
PspPI GGNCC 2 cut(s) 456, 1013
PspPPI RGGWCCY 1 cut(s) 1013
PstNI CAGNNNCTG 1 cut(s) 278
RsaI GTAC 4 cut(s) 340, 393, 841, 1047
RsaNI GTAC 4 cut(s) 339, 392, 840, 1046
RseI CAYNNNNRTG 4 cut(s) 99, 251, 423, 1275
SaqAI TTAA 9 cut(s) 20, 84, 357, 747, 879, 1094, 1254, 1532, 1591
SatI GCNGC 2 cut(s) 7, 1150
Sau3AI GATC 3 cut(s) 586, 942, 1159
Sau96I GGNCC 2 cut(s) 456, 1013
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 1 cut(s) 734
SduI GDGCHC 2 cut(s) 1143, 1552
SfaNI GCATC 3 cut(s) 586, 897, 1589
SinI GGWCC 1 cut(s) 1013
SmiMI CAYNNNNRTG 4 cut(s) 99, 251, 423, 1275
SmlI CTYRAG 2 cut(s) 830, 983
SmoI CTYRAG 2 cut(s) 830, 983
SpeI ACTAGT 2 cut(s) 536, 847
SsiI CCGC 1 cut(s) 1090
SspMI CTAG 2 cut(s) 537, 848
TaaI ACNGT 2 cut(s) 204, 1232
TaiI ACGT 1 cut(s) 897
TaqI TCGA 2 cut(s) 466, 1511
TatI WGTACW 1 cut(s) 1045
TfiI GAWTC 2 cut(s) 275, 790
Tru1I TTAA 9 cut(s) 20, 84, 357, 747, 879, 1094, 1254, 1532, 1591
Tru9I TTAA 9 cut(s) 20, 84, 357, 747, 879, 1094, 1254, 1532, 1591
TscAI CASTG 4 cut(s) 142, 207, 1035, 1057
TseFI GTSAC 4 cut(s) 106, 1030, 1390, 1574
TseI GCWGC 2 cut(s) 6, 1149
Tsp45I GTSAC 4 cut(s) 106, 1030, 1390, 1574
TspRI CASTG 4 cut(s) 142, 207, 1035, 1057
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 6 cut(s) 80, 90, 213, 482, 1068, 1478
XceI RCATGY 3 cut(s) 366, 1059, 1363
XspI CTAG 2 cut(s) 537, 848
ZrmI AGTACT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.