RLG00000009137

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005202
Physical Location & Seq
Forward (+)
47249582 .. 47251108
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009137

Sequence Viewer

Length: 1527 bp
ATGAACCAACTCAAGCAAATCCATGCCCACACACTCATCACCGGCCTAGGTCGTTTCACCTACATCACCAGCAAGCTCTTAGCGTTCTCCGCCTTATCGGAACACGGTGATTTACGCCACGCCGAGACTCTATTCAATCGAATTCCGGCACCCAATGTCTTTGATTTCAATTCCATGATCACGGGTTTCTTGAAAAACTCACACTCTGAAAAGGGTGGTCTCTCTGTTTTTACCAAAATGAGAAGGATTGGCGCTGACCCAAATGCTCGAACTTTCACAGTTTTGGTTAAAGCTTGTGTTGGGGTTTCTTTGCTTGATCAGATATATGGTCAGATAATGAAGTTTGGACATGGGTGTGATGTTTATGTTACTAGCTCAGTTATCAGTGTGTACTTTAAATGGAGAGCTGTGGAATTTGCTCGGAGAGTGTTTGATGAAAGTTTGGATAGGAATGTGGTGTGTTGGACTAGTCTTGTTAGCGGGTATTGCGGCAATGGGTTGGTTGATGATGCCCGTGACCTGTTCGATAAAATGCCTGGGAGAAATGATGTGTCTTACAGTGCAATGGTTTCGGGTTATGTTCAGAATGCTTGTTTCAACAAAGCTATTGATTTGTTTCGTGATTTCAAGCGTCGTCGTGCGAATGTGAGGTTGAAAGAGTCTCTTTTGGTTAGTGTTCTTGATGCCTGTGCTTCTGTGGGTGCATTCGAGGAAGGGAAGTGGATTCAGATGTATTTGGATGACCATGGTTTTGAGTATGGACTTGAGCTCGGTACGGCAGGAATAGATTTTTATGCAAAATGTGGATTCATTAAAGATGCTACAGAGATTTTTGATAAGATGCCGCGTAAAGATGTAACTACTTGGAGTGCTATTATCATGGGATATGCTATTAATGGTGAGAATGATACAGGGCTTGAACTTTTTGCTGAAATGGAAAAGAAGGGACCTAAACCAAATGCTGTGACATTTGTTGGAGTTCTCACTGCATGTAATCACAAAACTCTGGTAAATGAAGCATGGCGAGTATTGGGACGTATGAGTAAAGTTTATAGCATTGCTCCAGAGATTGAGCACTATGGGTGCATGGTTGATCTCTTGGCCCGTGCTGGGCGGGTGAATGAGGCAGAGATATTGATAAGGAGTATGGCAATGGAACCAGACGGAGCTATATGGGGGTCTTTACTTAACGGGTGTCTGATGCATGGCCATTTTGAGTTAGCCGGTAAGGTTGGGAAGCTTCTCATTCAATTAGAGCCTCAACATAGTGGAAGGTATGTTCTTTTAGCGAACATGTATGCCAAAATGGAAAACTGGGAGGATGTGTTGAGGCTAAGGAAAATGATGAAGGATAGAGAGATAGTTTCAATTTCTGCTTGGAGTTTCGTTGAGATTGATGGTGTTGTGCATAAATTCTTAGCCAATGATAAGTCTCATTCTCATTCAAGAAGTATATACCAAACATTAAATCAACTAAGCAAGGAACTCGAAAGTTTTTCCAATGCCAATGATTCTTTGCTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

509

Amino Acids

57.01

Weight (kDa)

7.23

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 51 - 100 7.2e-08 PPR repeat family
PPR_1 PF12854 148 - 178 2.2e-09 PPR repeat
PPR_2 PF13041 150 - 181 1.5e-06 PPR repeat family
PPR PF01535 153 - 181 2.4e-07 PPR repeat
PPR_2 PF13041 284 - 331 1e-09 PPR repeat family
E_motif PF20431 403 - 455 6.9e-11 E motif
Eplus_motif PF20430 469 - 496 1.7e-07 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 148
AccII CGCG 1 cut(s) 847
AciI CCGC 5 cut(s) 90, 480, 489, 845, 1114
AcoI YGGCCR 1 cut(s) 1207
AcsI RAATTY 3 cut(s) 141, 413, 1412
AfaI GTAC 2 cut(s) 392, 775
AfiI CCNNNNNNNGG 1 cut(s) 1110
AflIII ACRYGT 1 cut(s) 1293
AhlI ACTAGT 1 cut(s) 467
AjnI CCWGG 1 cut(s) 535
AloI GAACNNNNNNTCC 2 cut(s) 1263, 1295
AluBI AGCT 8 cut(s) 76, 293, 375, 407, 605, 769, 1169, 1240
AluI AGCT 8 cut(s) 76, 293, 375, 407, 605, 769, 1169, 1240
Alw21I GWGCWC 2 cut(s) 771, 1077
Alw26I GTCTC 4 cut(s) 119, 224, 666, 1437
AoxI GGCC 3 cut(s) 43, 1101, 1207
ApoI RAATTY 3 cut(s) 141, 413, 1412
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AseI ATTAAT 1 cut(s) 894
AspA2I CCTAGG 1 cut(s) 46
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 2 cut(s) 947, 1102
AsuHPI GGTGA 6 cut(s) 31, 49, 58, 119, 911, 1129
AvaII GGWCC 1 cut(s) 947
AvrII CCTAGG 1 cut(s) 46
BalI TGGCCA 1 cut(s) 1209
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 771
Bbv12I GWGCWC 2 cut(s) 771, 1077
BccI CCATC 1 cut(s) 1391
BceAI ACGGC 1 cut(s) 792
BcgI CGANNNNNNTGC 4 cut(s) 552, 586, 1468, 1502
BciT130I CCWGG 1 cut(s) 537
BclI TGATCA 2 cut(s) 177, 316
BcoDI GTCTC 4 cut(s) 119, 224, 666, 1437
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 3 cut(s) 47, 372, 468
BfmI CTRYAG 1 cut(s) 822
BfoI RGCGCY 1 cut(s) 255
BisI GCNGC 2 cut(s) 490, 845
BlnI CCTAGG 1 cut(s) 46
BlsI GCNGC 2 cut(s) 491, 846
Bme1390I CCNGG 1 cut(s) 537
Bme18I GGWCC 1 cut(s) 947
BmgT120I GGNCC 2 cut(s) 947, 1102
BmiI GGNNCC 3 cut(s) 150, 948, 1158
BmrFI CCNGG 1 cut(s) 537
BmrI ACTGGG 1 cut(s) 1324
BmsI GCATC 5 cut(s) 499, 673, 808, 831, 1191
BmuI ACTGGG 1 cut(s) 1324
BpmI CTGGAG 1 cut(s) 1047
Bpu10I CCTNAGC 1 cut(s) 1334
BpuEI CTTGAG 1 cut(s) 785
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 46, 536, 745
BsaXI ACNNNNNCTCC 2 cut(s) 1310, 1340
Bsc4I CCNNNNNNNGG 1 cut(s) 1110
Bse118I RCCGGY 2 cut(s) 41, 1223
Bse1I ACTGG 1 cut(s) 1319
Bse3DI GCAATG 4 cut(s) 499, 570, 1056, 1158
BseBI CCWGG 1 cut(s) 537
BseDI CCNNGG 3 cut(s) 46, 536, 745
BseGI GGATG 2 cut(s) 745, 1327
BseLI CCNNNNNNNGG 1 cut(s) 1110
BseMI GCAATG 4 cut(s) 499, 570, 1056, 1158
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 1319
BseYI CCCAGC 1 cut(s) 1109
Bsh1236I CGCG 1 cut(s) 847
BshFI GGCC 3 cut(s) 45, 1103, 1209
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 771, 1077
BsiSI CCGG 3 cut(s) 42, 146, 1224
BslFI GGGAC 2 cut(s) 960, 1047
BslI CCNNNNNNNGG 1 cut(s) 1110
BsmAI GTCTC 4 cut(s) 119, 224, 666, 1437
BsmFI GGGAC 2 cut(s) 960, 1047
BsmI GAATGC 2 cut(s) 592, 704
BsnI GGCC 3 cut(s) 45, 1103, 1209
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 771, 1077
Bsp143I GATC 3 cut(s) 177, 316, 1093
Bsp19I CCATGG 1 cut(s) 745
BspACI CCGC 5 cut(s) 90, 480, 489, 845, 1114
BspANI GGCC 3 cut(s) 45, 1103, 1209
BspCNI CTCAG 1 cut(s) 389
BspFNI CGCG 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 150, 948, 1158
BspT107I GGYRCC 1 cut(s) 148
BspTNI GGTCTC 1 cut(s) 224
BsrDI GCAATG 4 cut(s) 499, 570, 1056, 1158
BsrFI RCCGGY 2 cut(s) 41, 1223
BsrI ACTGG 1 cut(s) 1319
BssAI RCCGGY 2 cut(s) 41, 1223
BssECI CCNNGG 3 cut(s) 46, 536, 745
BssMI GATC 3 cut(s) 177, 316, 1093
BssT1I CCWWGG 2 cut(s) 46, 745
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 3 cut(s) 107, 280, 560
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 5 cut(s) 79, 376, 1334, 1417, 1475
BstDSI CCRYGG 1 cut(s) 745
BstF5I GGATG 2 cut(s) 745, 1327
BstFNI CGCG 1 cut(s) 847
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 3 cut(s) 180, 319, 1096
BstMAI GTCTC 4 cut(s) 119, 224, 666, 1437
BstMBI GATC 3 cut(s) 177, 316, 1093
BstMWI GCNNNNNNNGC 2 cut(s) 89, 486
BstNI CCWGG 1 cut(s) 537
BstNSI RCATGY 2 cut(s) 993, 1297
BstSCI CCNGG 1 cut(s) 535
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 1 cut(s) 847
BsuRI GGCC 3 cut(s) 45, 1103, 1209
BtgI CCRYGG 1 cut(s) 745
BtsCI GGATG 2 cut(s) 745, 1327
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 3 cut(s) 391, 565, 984
Cac8I GCNNGC 1 cut(s) 74
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 2 cut(s) 41, 1223
Cfr13I GGNCC 2 cut(s) 947, 1102
CseI GACGC 1 cut(s) 620
Csp6I GTAC 2 cut(s) 391, 774
CviQI GTAC 2 cut(s) 391, 774
DdeI CTNAG 5 cut(s) 79, 376, 1334, 1417, 1475
DpnI GATC 3 cut(s) 179, 318, 1095
DpnII GATC 3 cut(s) 177, 316, 1093
DraI TTTAAA 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 1207
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 46, 745
Eco24I GRGCYC 1 cut(s) 771
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 947
Eco53kI GAGCTC 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 769
EcoO109I RGGNCCY 1 cut(s) 947
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 46, 745
EcoT22I ATGCAT 1 cut(s) 1206
EcoT38I GRGCYC 1 cut(s) 771
ErhI CCWWGG 2 cut(s) 46, 745
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FaqI GGGAC 2 cut(s) 960, 1047
FauI CCCGC 2 cut(s) 473, 1107
FbaI TGATCA 2 cut(s) 177, 316
Fnu4HI GCNGC 2 cut(s) 490, 845
FokI GGATG 2 cut(s) 752, 1334
FriOI GRGCYC 1 cut(s) 771
Fsp4HI GCNGC 2 cut(s) 490, 845
FspBI CTAG 3 cut(s) 47, 372, 468
GlaI GCGC 1 cut(s) 253
GluI GCNGC 2 cut(s) 490, 845
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 3 cut(s) 45, 1103, 1209
HapII CCGG 3 cut(s) 42, 146, 1224
HgaI GACGC 1 cut(s) 620
HhaI GCGC 1 cut(s) 254
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HindIII AAGCTT 2 cut(s) 291, 1238
HinfI GANTC 5 cut(s) 127, 659, 724, 807, 1511
HpaII CCGG 3 cut(s) 42, 146, 1224
HphI GGTGA 6 cut(s) 31, 49, 58, 119, 911, 1129
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 8 cut(s) 100, 208, 321, 333, 423, 585, 729, 1200
Hpy188III TCNNGA 5 cut(s) 190, 620, 680, 1064, 1446
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 2 cut(s) 636, 639
HpyAV CCTTC 5 cut(s) 237, 707, 937, 1266, 1342
HpyCH4III ACNGT 3 cut(s) 107, 280, 560
HpyCH4IV ACGT 1 cut(s) 1036
HpyCH4V TGCA 7 cut(s) 563, 704, 797, 989, 1086, 1204, 1408
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 486
HpyF3I CTNAG 5 cut(s) 79, 376, 1334, 1417, 1475
HpySE526I ACGT 1 cut(s) 1036
HspAI GCGC 1 cut(s) 252
Ksp22I TGATCA 2 cut(s) 177, 316
Kzo9I GATC 3 cut(s) 177, 316, 1093
LmnI GCTCC 2 cut(s) 1066, 1166
LweI GCATC 5 cut(s) 499, 673, 808, 831, 1191
MaeI CTAG 3 cut(s) 47, 372, 468
MaeII ACGT 1 cut(s) 1036
MaeIII GTNAC 4 cut(s) 367, 515, 856, 964
MalI GATC 3 cut(s) 179, 318, 1095
MboI GATC 3 cut(s) 177, 316, 1093
MhlI GDGCHC 2 cut(s) 771, 1077
MlsI TGGCCA 1 cut(s) 1209
MluCI AATT 6 cut(s) 141, 169, 413, 1250, 1368, 1412
MluNI TGGCCA 1 cut(s) 1209
MlyI GAGTC 2 cut(s) 121, 668
MmeI TCCRAC 2 cut(s) 443, 955
MnlI CCTC 6 cut(s) 642, 703, 1117, 1269, 1312, 1323
Mox20I TGGCCA 1 cut(s) 1209
Mph1103I ATGCAT 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 1209
MseI TTAA 6 cut(s) 288, 396, 813, 894, 1188, 1466
MslI CAYNNNNRTG 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 1209
MspI CCGG 3 cut(s) 42, 146, 1224
MspR9I CCNGG 1 cut(s) 537
Mva1269I GAATGC 2 cut(s) 592, 704
MvaI CCWGG 1 cut(s) 537
MvnI CGCG 1 cut(s) 847
MwoI GCNNNNNNNGC 2 cut(s) 89, 486
NcoI CCATGG 1 cut(s) 745
NdeII GATC 3 cut(s) 177, 316, 1093
NlaIV GGNNCC 3 cut(s) 150, 948, 1158
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 515, 964
NsiI ATGCAT 1 cut(s) 1206
NspI RCATGY 2 cut(s) 993, 1297
PciI ACATGT 1 cut(s) 1293
PctI GAATGC 2 cut(s) 592, 704
PfeI GAWTC 3 cut(s) 724, 807, 1511
PkrI GCNGC 2 cut(s) 491, 846
PleI GAGTC 2 cut(s) 121, 667
PpsI GAGTC 2 cut(s) 121, 667
PpuMI RGGWCCY 1 cut(s) 947
PscI ACATGT 1 cut(s) 1293
PshBI ATTAAT 1 cut(s) 894
Psp124BI GAGCTC 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 1 cut(s) 535
PspN4I GGNNCC 3 cut(s) 150, 948, 1158
PspPI GGNCC 2 cut(s) 947, 1102
PspPPI RGGWCCY 1 cut(s) 947
RsaI GTAC 2 cut(s) 392, 775
RsaNI GTAC 2 cut(s) 391, 774
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 771
SaqAI TTAA 6 cut(s) 288, 396, 813, 894, 1188, 1466
SatI GCNGC 2 cut(s) 490, 845
Sau3AI GATC 3 cut(s) 177, 316, 1093
Sau96I GGNCC 2 cut(s) 947, 1102
SchI GAGTC 2 cut(s) 121, 668
ScrFI CCNGG 1 cut(s) 537
SduI GDGCHC 2 cut(s) 771, 1077
SfaNI GCATC 5 cut(s) 499, 673, 808, 831, 1191
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 1 cut(s) 947
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 2 cut(s) 11, 764
SmoI CTYRAG 2 cut(s) 11, 764
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 6 cut(s) 141, 169, 413, 1250, 1368, 1412
SsiI CCGC 5 cut(s) 90, 480, 489, 845, 1114
SspMI CTAG 3 cut(s) 47, 372, 468
SstI GAGCTC 1 cut(s) 771
StyD4I CCNGG 1 cut(s) 535
StyI CCWWGG 2 cut(s) 46, 745
TaaI ACNGT 3 cut(s) 107, 280, 560
TaiI ACGT 1 cut(s) 1039
TaqI TCGA 5 cut(s) 139, 268, 525, 708, 1488
TasI AATT 6 cut(s) 141, 169, 413, 1250, 1368, 1412
TatI WGTACW 1 cut(s) 390
TauI GCSGC 2 cut(s) 492, 847
TfiI GAWTC 3 cut(s) 724, 807, 1511
Tru1I TTAA 6 cut(s) 288, 396, 813, 894, 1188, 1466
Tru9I TTAA 6 cut(s) 288, 396, 813, 894, 1188, 1466
TscAI CASTG 3 cut(s) 391, 565, 991
TseFI GTSAC 2 cut(s) 515, 964
Tsp45I GTSAC 2 cut(s) 515, 964
TspDTI ATGAA 6 cut(s) 17, 353, 450, 799, 1029, 1361
TspGWI ACGGA 1 cut(s) 1179
TspRI CASTG 3 cut(s) 391, 565, 991
VpaK11BI GGWCC 1 cut(s) 947
VspI ATTAAT 1 cut(s) 894
XapI RAATTY 3 cut(s) 141, 413, 1412
XceI RCATGY 2 cut(s) 993, 1297
XmaJI CCTAGG 1 cut(s) 46
XspI CTAG 3 cut(s) 47, 372, 468
Zsp2I ATGCAT 1 cut(s) 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.