MD13G1252400.v1.1

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005202
Physical Location & Seq
Reverse (-)
26926702 .. 26929767
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1252400.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGGGCAGCAAAATGGTTTTAAAACCAGTAACTTGTCCTCCATATCTTCTCCTACTACAAAGATGTTCAATCTTGGACGAACTTAAGAAAATCCATGCCCATGCAGTCACTACTGGTCTTGCTCGTTTTGCCTACACTGCCAGCAAGCTCGTAGCCTTCTCTGCATTATCAGAACATGGTAATATGCACTATGCTGAGACACTGTCACACCAAATTCCAAGACCCAATGTATTTGATTACAATTCCATGATAATGGGTTTCTTGAAAAGCTCAGAATCTGAAAAGGGTCTCTCCATTTACACCAGAATGAGAAGGATAGGCACAGAGCCAAACGCTTGCACTTTCACCTTTTTAGTTAAAGCATGTCTTGATGTATCTTTGCTTGGTCAGGTACATGGTCAAATAATGAAGTTTGGGCATGGGTGTGATGTTTATGTTATTAGCTCACTTATTAGGGCCTCCTGTCGATGTGAAGCTGTGGAATTTGCTCGTAGAGTGTTTGGGGAAAGTGTGGATAAGAATGTGGTTTGTTGGACTAGTCTTATTAGTGGATATTGTAGTAATGGGCTGGTTCATGAAGCTCGTGATCTGTTTGATGCAATGCCGGATATAAATGATGTTTCTTATAGTGCAATGGTTTCTGGGTATGTTGGGAATGCTTGTTTCAATGAAGCAATTGACCTGTTTCGTCAATTGAAAAGCCATACACAAGTGAGGCTCAAAGAGTCTCTTTTGGTTAGCACTCTTAATGCTTGTGCTTCTGTGGGTGCCTTTAAAGAAGGGAAGTGGATTCATTCGTTTTTGGAAGAAAATGGTTTTGAGTATGGACTTGAGCTTGGTACTGCACTAGTAGATTTTTATGCAAAATGTGGATGTATTAAAGACGCACAAGACGTGTTTAGCAGGATGCCTCGTAAAGATGTAACTACTTTGAGTGCTATGATCATGGGATTTGCCATCAATGGTGAGAATGATATGGGGCTTGAGCTTTTTGCAGAAATGGAAAAGAAAGGACCTAAACCAAATGCAGTGACATTTGTTGGAGTACTCACTGCATGTAATCACAAAATTCTGGTAAATGAAGCATGGCGGTTAATAGGACGCATGAGTAAAGTTTATGGCATTGCTCTGGGGATTGAGCACTATGGCTGCATGGTTGATCTCTTGGCCCGTGCTGGGAAATTGAATGAAGCAGAGGTATTGATAAGAAGTATGGCAATGAAACCGGACGGTGCCATTTGGGGGTCTTTGTTTAATGGATGTCTGATGCATGACCATGTTGACTTAGGGGCGAGGGTGGGGAAGCTTTTGATTGAATTAGAGCCTCAACATAGTGGTAGGTATGTTCTTCTGGCCAACATGTACGCCAAAATGGGCAACTGGGAGGATGTGACGAGGCTAAGGAAAATGATGAGAGATAGAGAAGTAGTTACAATTTCTGCTTGGAGCTTCATTGAGACTGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

490

Amino Acids

54.35

Weight (kDa)

6.93

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 74 - 122 8.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 75 - 122 2.6e-09 PPR repeat family
PPR_2 PF13041 173 - 202 6.7e-07 PPR repeat family
PPR_1 PF12854 174 - 201 2.1e-09 PPR repeat
PPR PF01535 176 - 202 4.8e-08 PPR repeat
PPR_2 PF13041 306 - 353 2.5e-08 PPR repeat family
E_motif PF20431 424 - 476 5.9e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 767, 1232
AciI CCGC 1 cut(s) 1090
AcoI YGGCCR 1 cut(s) 1353
AcsI RAATTY 3 cut(s) 213, 482, 1068
AfaI GTAC 4 cut(s) 393, 841, 1047, 1364
AfiI CCNNNNNNNGG 2 cut(s) 1176, 1242
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 2 cut(s) 894, 1359
AgsI TTSAA 6 cut(s) 69, 265, 667, 697, 1186, 1316
AhlI ACTAGT 2 cut(s) 536, 847
AjiI CACGTC 1 cut(s) 895
AluBI AGCT 9 cut(s) 148, 270, 444, 476, 581, 835, 988, 1306, 1449
AluI AGCT 9 cut(s) 148, 270, 444, 476, 581, 835, 988, 1306, 1449
Alw21I GWGCWC 1 cut(s) 1143
Alw26I GTCTC 4 cut(s) 191, 293, 732, 1451
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 3 cut(s) 456, 1167, 1353
ApeKI GCWGC 2 cut(s) 6, 1149
ApoI RAATTY 3 cut(s) 213, 482, 1068
AspS9I GGNCC 3 cut(s) 456, 1013, 1168
AsuHPI GGTGA 2 cut(s) 337, 977
AvaII GGWCC 1 cut(s) 1013
BalI TGGCCA 1 cut(s) 1355
BanI GGYRCC 2 cut(s) 767, 1232
BauI CACGAG 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 1143
BbvI GCAGC 2 cut(s) 18, 1136
BccI CCATC 1 cut(s) 965
BclI TGATCA 1 cut(s) 942
BcoDI GTCTC 4 cut(s) 191, 293, 732, 1451
BcuI ACTAGT 2 cut(s) 536, 847
BfaI CTAG 2 cut(s) 537, 848
BfrI CTTAAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 7, 1150
BlsI GCNGC 2 cut(s) 8, 1151
BmcAI AGTACT 1 cut(s) 1047
Bme18I GGWCC 1 cut(s) 1013
BmgBI CACGTC 1 cut(s) 895
BmgT120I GGNCC 3 cut(s) 456, 1013, 1168
BmiI GGNNCC 2 cut(s) 769, 1234
BmrI ACTGGG 1 cut(s) 1390
BmsI GCATC 3 cut(s) 586, 897, 1257
BmuI ACTGGG 1 cut(s) 1390
Bpu10I CCTNAGC 1 cut(s) 1400
BpuEI CTTGAG 2 cut(s) 851, 1004
BsaI GGTCTC 1 cut(s) 293
BsaWI WCCGGW 1 cut(s) 1225
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 2 cut(s) 1176, 1242
Bse1I ACTGG 3 cut(s) 26, 118, 1385
Bse3DI GCAATG 4 cut(s) 606, 639, 1122, 1224
BseGI GGATG 4 cut(s) 878, 912, 1265, 1393
BseLI CCNNNNNNNGG 2 cut(s) 1176, 1242
BseMI GCAATG 4 cut(s) 606, 639, 1122, 1224
BseMII CTCAG 2 cut(s) 186, 285
BseNI ACTGG 3 cut(s) 26, 118, 1385
BseXI GCAGC 2 cut(s) 18, 1136
BseYI CCCAGC 1 cut(s) 1175
BsgI GTGCAG 1 cut(s) 828
BshFI GGCC 3 cut(s) 458, 1169, 1355
BshNI GGYRCC 2 cut(s) 767, 1232
BsiHKAI GWGCWC 1 cut(s) 1143
BsiSI CCGG 2 cut(s) 605, 1226
BslI CCNNNNNNNGG 2 cut(s) 1176, 1242
BsmAI GTCTC 4 cut(s) 191, 293, 732, 1451
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 3 cut(s) 458, 1169, 1355
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 1143
Bsp143I GATC 3 cut(s) 586, 942, 1159
BspACI CCGC 1 cut(s) 1090
BspANI GGCC 3 cut(s) 458, 1169, 1355
BspCNI CTCAG 2 cut(s) 187, 284
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 2 cut(s) 769, 1234
BspT107I GGYRCC 2 cut(s) 767, 1232
BspTI CTTAAG 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 293
BsrDI GCAATG 4 cut(s) 606, 639, 1122, 1224
BsrI ACTGG 3 cut(s) 26, 118, 1385
BssMI GATC 3 cut(s) 586, 942, 1159
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 204, 1232
BstAFI CTTAAG 1 cut(s) 83
BstC8I GCNNGC 3 cut(s) 142, 146, 337
BstDEI CTNAG 4 cut(s) 195, 271, 1285, 1400
BstF5I GGATG 4 cut(s) 878, 912, 1265, 1393
BstKTI GATC 3 cut(s) 589, 945, 1162
BstMAI GTCTC 4 cut(s) 191, 293, 732, 1451
BstMBI GATC 3 cut(s) 586, 942, 1159
BstMWI GCNNNNNNNGC 3 cut(s) 128, 137, 161
BstNSI RCATGY 3 cut(s) 366, 1059, 1363
BstV1I GCAGC 2 cut(s) 18, 1136
BstXI CCANNNNNNTGG 1 cut(s) 253
BsuRI GGCC 3 cut(s) 458, 1169, 1355
BtrI CACGTC 1 cut(s) 895
BtsCI GGATG 4 cut(s) 878, 912, 1265, 1393
BtsI GCAGTG 3 cut(s) 135, 1035, 1050
BtsIMutI CAGTG 4 cut(s) 135, 200, 1035, 1050
Cac8I GCNNGC 3 cut(s) 142, 146, 337
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 574
Cfr13I GGNCC 3 cut(s) 456, 1013, 1168
CseI GACGC 2 cut(s) 893, 1110
Csp6I GTAC 4 cut(s) 392, 840, 1046, 1363
CviQI GTAC 4 cut(s) 392, 840, 1046, 1363
DdeI CTNAG 4 cut(s) 195, 271, 1285, 1400
DpnI GATC 3 cut(s) 588, 944, 1161
DpnII GATC 3 cut(s) 586, 942, 1159
DraI TTTAAA 2 cut(s) 21, 775
EaeI YGGCCR 1 cut(s) 1353
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 1013
EcoO109I RGGNCCY 2 cut(s) 456, 1013
EcoT22I ATGCAT 1 cut(s) 1272
FalI AAGNNNNNCTT 4 cut(s) 351, 383, 714, 746
FbaI TGATCA 1 cut(s) 942
Fnu4HI GCNGC 2 cut(s) 7, 1150
FokI GGATG 4 cut(s) 885, 919, 1272, 1400
Fsp4HI GCNGC 2 cut(s) 7, 1150
FspBI CTAG 2 cut(s) 537, 848
GluI GCNGC 2 cut(s) 7, 1150
GsaI CCCAGC 1 cut(s) 1179
HaeIII GGCC 3 cut(s) 458, 1169, 1355
HapII CCGG 2 cut(s) 605, 1226
HgaI GACGC 2 cut(s) 893, 1110
HincII GTYRAC 1 cut(s) 1282
HindII GTYRAC 1 cut(s) 1282
HindIII AAGCTT 1 cut(s) 1304
HinfI GANTC 3 cut(s) 275, 725, 790
HpaII CCGG 2 cut(s) 605, 1226
HphI GGTGA 2 cut(s) 337, 977
Hpy166II GTNNAC 1 cut(s) 1282
Hpy188I TCNGA 4 cut(s) 172, 274, 280, 1266
Hpy188III TCNNGA 4 cut(s) 262, 368, 575, 584
Hpy8I GTNNAC 1 cut(s) 1282
HpyAV CCTTC 3 cut(s) 166, 306, 773
HpyCH4III ACNGT 2 cut(s) 204, 1232
HpyCH4IV ACGT 1 cut(s) 894
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 137, 161
HpyF3I CTNAG 4 cut(s) 195, 271, 1285, 1400
HpySE526I ACGT 1 cut(s) 894
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 3 cut(s) 586, 942, 1159
LmnI GCTCC 1 cut(s) 1446
Lsp1109I GCAGC 2 cut(s) 18, 1136
LweI GCATC 3 cut(s) 586, 897, 1257
MaeI CTAG 2 cut(s) 537, 848
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 7 cut(s) 28, 106, 204, 922, 1030, 1390, 1429
MalI GATC 3 cut(s) 588, 944, 1161
MboI GATC 3 cut(s) 586, 942, 1159
MboII GAAGA 3 cut(s) 38, 818, 1340
MfeI CAATTG 2 cut(s) 675, 692
MhlI GDGCHC 1 cut(s) 1143
MlsI TGGCCA 1 cut(s) 1355
MluCI AATT 9 cut(s) 213, 241, 482, 675, 692, 1068, 1181, 1316, 1434
MluNI TGGCCA 1 cut(s) 1355
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 512, 1021
MnlI CCTC 9 cut(s) 48, 469, 708, 921, 1189, 1287, 1335, 1378, 1389
Mox20I TGGCCA 1 cut(s) 1355
Mph1103I ATGCAT 1 cut(s) 1272
MscI TGGCCA 1 cut(s) 1355
MseI TTAA 8 cut(s) 20, 84, 357, 747, 774, 879, 1094, 1254
MslI CAYNNNNRTG 5 cut(s) 99, 251, 305, 423, 1275
Msp20I TGGCCA 1 cut(s) 1355
MspCI CTTAAG 1 cut(s) 83
MspI CCGG 2 cut(s) 605, 1226
MunI CAATTG 2 cut(s) 675, 692
Mva1269I GAATGC 1 cut(s) 661
MwoI GCNNNNNNNGC 3 cut(s) 128, 137, 161
NdeII GATC 3 cut(s) 586, 942, 1159
NlaIV GGNNCC 2 cut(s) 769, 1234
NmuCI GTSAC 4 cut(s) 106, 204, 1030, 1390
NsiI ATGCAT 1 cut(s) 1272
NspI RCATGY 3 cut(s) 366, 1059, 1363
PagI TCATGA 1 cut(s) 574
PciI ACATGT 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 1 cut(s) 891
PctI GAATGC 1 cut(s) 661
PfeI GAWTC 2 cut(s) 275, 790
PflFI GACNNNGTC 1 cut(s) 202
PkrI GCNGC 2 cut(s) 8, 1151
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 1013
PscI ACATGT 1 cut(s) 1359
Psp5II RGGWCCY 1 cut(s) 1013
PspFI CCCAGC 1 cut(s) 1175
PspN4I GGNNCC 2 cut(s) 769, 1234
PspPI GGNCC 3 cut(s) 456, 1013, 1168
PspPPI RGGWCCY 1 cut(s) 1013
PstNI CAGNNNCTG 1 cut(s) 278
PsyI GACNNNGTC 1 cut(s) 202
RsaI GTAC 4 cut(s) 393, 841, 1047, 1364
RsaNI GTAC 4 cut(s) 392, 840, 1046, 1363
RseI CAYNNNNRTG 5 cut(s) 99, 251, 305, 423, 1275
SaqAI TTAA 8 cut(s) 20, 84, 357, 747, 774, 879, 1094, 1254
SatI GCNGC 2 cut(s) 7, 1150
Sau3AI GATC 3 cut(s) 586, 942, 1159
Sau96I GGNCC 3 cut(s) 456, 1013, 1168
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 1 cut(s) 734
SduI GDGCHC 1 cut(s) 1143
SfaNI GCATC 3 cut(s) 586, 897, 1257
SinI GGWCC 1 cut(s) 1013
SmiMI CAYNNNNRTG 5 cut(s) 99, 251, 305, 423, 1275
SmlI CTYRAG 3 cut(s) 83, 830, 983
SmoI CTYRAG 3 cut(s) 83, 830, 983
SpeI ACTAGT 2 cut(s) 536, 847
Sse9I AATT 9 cut(s) 213, 241, 482, 675, 692, 1068, 1181, 1316, 1434
SsiI CCGC 1 cut(s) 1090
SspMI CTAG 2 cut(s) 537, 848
TaaI ACNGT 2 cut(s) 204, 1232
TaiI ACGT 1 cut(s) 897
TaqI TCGA 1 cut(s) 466
TasI AATT 9 cut(s) 213, 241, 482, 675, 692, 1068, 1181, 1316, 1434
TatI WGTACW 1 cut(s) 1045
TfiI GAWTC 2 cut(s) 275, 790
Tru1I TTAA 8 cut(s) 20, 84, 357, 747, 774, 879, 1094, 1254
Tru9I TTAA 8 cut(s) 20, 84, 357, 747, 774, 879, 1094, 1254
TscAI CASTG 4 cut(s) 142, 207, 1035, 1057
TseFI GTSAC 4 cut(s) 106, 204, 1030, 1390
TseI GCWGC 2 cut(s) 6, 1149
Tsp45I GTSAC 4 cut(s) 106, 204, 1030, 1390
TspDTI ATGAA 9 cut(s) 422, 563, 591, 684, 782, 1095, 1203, 1235, 1441
TspRI CASTG 4 cut(s) 142, 207, 1035, 1057
Tth111I GACNNNGTC 1 cut(s) 202
Vha464I CTTAAG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 3 cut(s) 213, 482, 1068
XceI RCATGY 3 cut(s) 366, 1059, 1363
XspI CTAG 2 cut(s) 537, 848
ZrmI AGTACT 1 cut(s) 1047
Zsp2I ATGCAT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.