pycom13g22560

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005202
Physical Location & Seq
Forward (+)
20172046 .. 20173638
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g22560.1

Sequence Viewer

Length: 1593 bp
ATGGGCAGCAAAGTCGTTTTAAAACCAGTAACTTGTCCTCCATATCTTCTCCTACTACAAAGATGTTCAAGCTTGGACGAACTTAAGAAAATCCATGCCCATGCAGTCACTACTGGTCTTGCTCGTTTTGCCTACACTGCCAGCAAGCTCTTAGCCTTCTCTGCATTATCAGAACATGGTAATATGCACTATGCTGAGACACTGTTACACCAAATTCCAAGACCCAATGTATTTGATTACAATTCCATGATAATGGGTTTCTTGAAGAGCTCAGAATCTGAAAAGGGTGTCTCCATTTACACCAGAATGAGAAGGATAGGCACAGAGCCAAACGCTCGTACTTTCACCTTTGTAGTTAAAGCATGTCTTGATGTATCTTTGCTTGGTCAGGTACATGGTCAAATAATGAAGTTTGGGCATGGGTGTGATGTTTATGTTATTAGCTCACTTATTAGGGCCTCATGTCGATGTGAAGCTGTGGAATTTGCTCATAGAGTGTTTGAGGAAAGTTTGGATAAGAATGTGGTTTGTTGGACTAGTCTTATTAGTGGATATTGTAGTAATGGGCTGGTTCATGAAGCTCGTAATCTGTTTGATGCAATGCCGGATATAAATGATGTTTCTTATAGTGCAATGGTTTCTGGGTATGTTGGGAATGCTTGTTTCAATGAAGCAATTGATCTGTTTCGTCAATTGAAAAGCCATACACAAGTGAGGCTCAATGAGTCTCTTTTGGTTAGCACTCTTAATGCTTGTGCATCTGTGGGTGCCTTTGAAGAAGGGAAGTGGATTCATTCGTTTTTGGAAGAAAATGGTTTTGAGTATGGACTTGAGCTTGGTACTGCACTAGTAGATTTTTATGCAAAATGTGGATGTATTAAAGACGCACAAGATGTGTTTAGCAAGATGCCTTGTAAAGATGTAACTACTTGGAGTGCTATGATCATGGGATTTGCCATCAATGGTGAGAATGATATGGGACTTGAGCTTTTTGCAGAAATGGAAAAGAAAGGACCTAAACCAAATGCAGTGACATTTGTTGGAGTACTCACTGCATGTAATCACAAAATTCTGGTAAATGAAGCATGGTGGTTGATAGGACGCATGAGTAAAGTTTATGGCATTGCTCTGGGGATTGAGCACTATGGCTGCATGGTTGATCTCTTGGCCCGTGCTGGGAAACTGAATGAAGCAGAGATATTGATAAGAAGTATGGCAATGAAAGCGGACGGTGCTATTTGGGGGTCTTTGTTCAATGGATGTGTGATACATGACCAAGTTGACTTAGGGGCGAGGGTGGGGAAGCTTTTGATTGAATTAGAGCCTCAACATAGTGGTAGGTATGTTCTTCTGGCCAACATGTATGCCAAAATGGGCAACTGGGAGGATGTGACGAGGCTAAGGAAAATGATGAGAGATAGAGAAGTAGGTACAATTTCAGCTTGGAGCTTCATTGAGACTGATGGTATTGTCCATAAATTCATAGCAGACGATAAGTCTCGTTCCCATTCGAGAAAGATAAACACAAGTTTAAACCAACTCAGCAGGGCACTTGAAAGTTTTTCCATTGCTAGTGACGCATCGTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

531

Amino Acids

58.82

Weight (kDa)

6.63

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 122 1.1e-08 PPR repeat family
PPR_2 PF13041 173 - 202 1.2e-06 PPR repeat family
PPR_1 PF12854 174 - 201 2.6e-09 PPR repeat
PPR PF01535 176 - 202 1.4e-07 PPR repeat
PPR_2 PF13041 306 - 353 9.1e-10 PPR repeat family
PPR PF01535 310 - 338 7.1e-06 PPR repeat
E_motif PF20431 424 - 476 4.9e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 767
AciI CCGC 1 cut(s) 1226
AcoI YGGCCR 1 cut(s) 1353
AcsI RAATTY 4 cut(s) 213, 482, 1068, 1478
AfaI GTAC 5 cut(s) 340, 393, 841, 1047, 1432
AfiI CCNNNNNNNGG 1 cut(s) 1176
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 1359
AgsI TTSAA 8 cut(s) 69, 265, 667, 697, 776, 1255, 1316, 1556
AhdI GACNNNNNGTC 1 cut(s) 1495
AhlI ACTAGT 2 cut(s) 536, 847
Alw21I GWGCWC 2 cut(s) 272, 1143
Alw26I GTCTC 5 cut(s) 191, 295, 732, 1451, 1503
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 3 cut(s) 456, 1167, 1353
ApeKI GCWGC 2 cut(s) 6, 1149
ApoI RAATTY 4 cut(s) 213, 482, 1068, 1478
AspS9I GGNCC 3 cut(s) 456, 1013, 1168
AsuHPI GGTGA 2 cut(s) 337, 977
AvaII GGWCC 1 cut(s) 1013
BaeGI GKGCMC 1 cut(s) 1552
BalI TGGCCA 1 cut(s) 1355
BanI GGYRCC 1 cut(s) 767
BanII GRGCYC 1 cut(s) 272
Bbv12I GWGCWC 2 cut(s) 272, 1143
BbvI GCAGC 2 cut(s) 18, 1136
BccI CCATC 2 cut(s) 965, 1457
BcgI CGANNNNNNTGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 942
BcoDI GTCTC 5 cut(s) 191, 295, 732, 1451, 1503
BcuI ACTAGT 2 cut(s) 536, 847
BfaI CTAG 3 cut(s) 537, 848, 1572
BfrI CTTAAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 7, 1150
BlsI GCNGC 2 cut(s) 8, 1151
BmcAI AGTACT 1 cut(s) 1047
Bme18I GGWCC 1 cut(s) 1013
BmeRI GACNNNNNGTC 1 cut(s) 1495
BmgT120I GGNCC 3 cut(s) 456, 1013, 1168
BmiI GGNNCC 1 cut(s) 769
BmrI ACTGGG 1 cut(s) 1390
BmsI GCATC 4 cut(s) 586, 767, 897, 1589
BmuI ACTGGG 1 cut(s) 1390
Bpu10I CCTNAGC 1 cut(s) 1400
BpuEI CTTGAG 2 cut(s) 851, 1004
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 1176
Bse1I ACTGG 3 cut(s) 26, 118, 1385
Bse3DI GCAATG 5 cut(s) 606, 639, 1122, 1224, 1566
BseGI GGATG 3 cut(s) 878, 1265, 1393
BseLI CCNNNNNNNGG 1 cut(s) 1176
BseMI GCAATG 5 cut(s) 606, 639, 1122, 1224, 1566
BseMII CTCAG 3 cut(s) 186, 285, 1555
BseNI ACTGG 3 cut(s) 26, 118, 1385
BseSI GKGCMC 1 cut(s) 1552
BseXI GCAGC 2 cut(s) 18, 1136
BseYI CCCAGC 1 cut(s) 1175
BsgI GTGCAG 1 cut(s) 828
BshFI GGCC 3 cut(s) 458, 1169, 1355
BshNI GGYRCC 1 cut(s) 767
BsiHKAI GWGCWC 2 cut(s) 272, 1143
BsiSI CCGG 1 cut(s) 605
BslFI GGGAC 1 cut(s) 993
BslI CCNNNNNNNGG 1 cut(s) 1176
BsmAI GTCTC 5 cut(s) 191, 295, 732, 1451, 1503
BsmFI GGGAC 1 cut(s) 993
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 3 cut(s) 458, 1169, 1355
Bsp1286I GDGCHC 3 cut(s) 272, 1143, 1552
Bsp143I GATC 3 cut(s) 679, 942, 1159
BspACI CCGC 1 cut(s) 1226
BspANI GGCC 3 cut(s) 458, 1169, 1355
BspCNI CTCAG 3 cut(s) 187, 284, 1554
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 769
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 767
BspTI CTTAAG 1 cut(s) 83
BsrDI GCAATG 5 cut(s) 606, 639, 1122, 1224, 1566
BsrI ACTGG 3 cut(s) 26, 118, 1385
BssMI GATC 3 cut(s) 679, 942, 1159
Bst4CI ACNGT 2 cut(s) 204, 1232
Bst6I CTCTTC 1 cut(s) 260
BstAFI CTTAAG 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 142, 146
BstDEI CTNAG 6 cut(s) 151, 195, 271, 1285, 1400, 1541
BstF5I GGATG 3 cut(s) 878, 1265, 1393
BstKTI GATC 3 cut(s) 682, 945, 1162
BstMAI GTCTC 5 cut(s) 191, 295, 732, 1451, 1503
BstMBI GATC 3 cut(s) 679, 942, 1159
BstMWI GCNNNNNNNGC 6 cut(s) 128, 137, 161, 1223, 1232, 1577
BstNSI RCATGY 3 cut(s) 366, 1059, 1363
BstSLI GKGCMC 1 cut(s) 1552
BstV1I GCAGC 2 cut(s) 18, 1136
BstXI CCANNNNNNTGG 1 cut(s) 253
BsuRI GGCC 3 cut(s) 458, 1169, 1355
BtsCI GGATG 3 cut(s) 878, 1265, 1393
BtsI GCAGTG 3 cut(s) 135, 1035, 1050
BtsIMutI CAGTG 4 cut(s) 135, 200, 1035, 1050
Cac8I GCNNGC 2 cut(s) 142, 146
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 574
Cfr13I GGNCC 3 cut(s) 456, 1013, 1168
CseI GACGC 3 cut(s) 893, 1110, 1586
Csp6I GTAC 5 cut(s) 339, 392, 840, 1046, 1431
CviQI GTAC 5 cut(s) 339, 392, 840, 1046, 1431
DdeI CTNAG 6 cut(s) 151, 195, 271, 1285, 1400, 1541
DpnI GATC 3 cut(s) 681, 944, 1161
DpnII GATC 3 cut(s) 679, 942, 1159
DraI TTTAAA 2 cut(s) 21, 1533
DriI GACNNNNNGTC 1 cut(s) 1495
EaeI YGGCCR 1 cut(s) 1353
Eam1104I CTCTTC 1 cut(s) 260
Eam1105I GACNNNNNGTC 1 cut(s) 1495
EarI CTCTTC 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 1013
Eco53kI GAGCTC 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 270
EcoO109I RGGNCCY 2 cut(s) 456, 1013
EcoT38I GRGCYC 1 cut(s) 272
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 1 cut(s) 993
FbaI TGATCA 1 cut(s) 942
Fnu4HI GCNGC 2 cut(s) 7, 1150
FokI GGATG 3 cut(s) 885, 1272, 1400
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 2 cut(s) 7, 1150
FspBI CTAG 3 cut(s) 537, 848, 1572
GluI GCNGC 2 cut(s) 7, 1150
GsaI CCCAGC 1 cut(s) 1179
HaeIII GGCC 3 cut(s) 458, 1169, 1355
HapII CCGG 1 cut(s) 605
HgaI GACGC 3 cut(s) 893, 1110, 1586
HincII GTYRAC 1 cut(s) 1282
HindII GTYRAC 1 cut(s) 1282
HindIII AAGCTT 2 cut(s) 70, 1304
HinfI GANTC 3 cut(s) 275, 725, 790
HpaII CCGG 1 cut(s) 605
HphI GGTGA 2 cut(s) 337, 977
Hpy166II GTNNAC 1 cut(s) 1282
Hpy188I TCNGA 3 cut(s) 172, 274, 280
Hpy188III TCNNGA 4 cut(s) 262, 368, 575, 1512
Hpy8I GTNNAC 1 cut(s) 1282
HpyAV CCTTC 3 cut(s) 166, 306, 773
HpyCH4III ACNGT 2 cut(s) 204, 1232
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 137, 161, 1223, 1232, 1577
HpyF3I CTNAG 6 cut(s) 151, 195, 271, 1285, 1400, 1541
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 3 cut(s) 679, 942, 1159
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 1 cut(s) 1446
Lsp1109I GCAGC 2 cut(s) 18, 1136
LweI GCATC 4 cut(s) 586, 767, 897, 1589
MaeI CTAG 3 cut(s) 537, 848, 1572
MaeIII GTNAC 8 cut(s) 28, 106, 204, 922, 1030, 1390, 1574, 1584
MalI GATC 3 cut(s) 681, 944, 1161
MboI GATC 3 cut(s) 679, 942, 1159
MboII GAAGA 5 cut(s) 38, 277, 788, 818, 1340
MfeI CAATTG 2 cut(s) 675, 692
MhlI GDGCHC 3 cut(s) 272, 1143, 1552
MlsI TGGCCA 1 cut(s) 1355
MluCI AATT 9 cut(s) 213, 241, 482, 675, 692, 1068, 1316, 1434, 1478
MluNI TGGCCA 1 cut(s) 1355
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 512, 1021
MnlI CCTC 8 cut(s) 48, 469, 496, 708, 1287, 1335, 1378, 1389
Mox20I TGGCCA 1 cut(s) 1355
MscI TGGCCA 1 cut(s) 1355
MseI TTAA 7 cut(s) 20, 84, 357, 747, 879, 1532, 1591
MslI CAYNNNNRTG 5 cut(s) 99, 251, 305, 423, 466
Msp20I TGGCCA 1 cut(s) 1355
MspCI CTTAAG 1 cut(s) 83
MspI CCGG 1 cut(s) 605
MssI GTTTAAAC 1 cut(s) 1533
MunI CAATTG 2 cut(s) 675, 692
Mva1269I GAATGC 1 cut(s) 661
MwoI GCNNNNNNNGC 6 cut(s) 128, 137, 161, 1223, 1232, 1577
NdeII GATC 3 cut(s) 679, 942, 1159
NlaIV GGNNCC 1 cut(s) 769
NmuCI GTSAC 4 cut(s) 106, 1030, 1390, 1574
NspI RCATGY 3 cut(s) 366, 1059, 1363
PagI TCATGA 1 cut(s) 574
PciI ACATGT 1 cut(s) 1359
PciSI GCTCTTC 1 cut(s) 260
PctI GAATGC 1 cut(s) 661
PfeI GAWTC 2 cut(s) 275, 790
PkrI GCNGC 2 cut(s) 8, 1151
PleI GAGTC 1 cut(s) 733
PmeI GTTTAAAC 1 cut(s) 1533
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 1013
PscI ACATGT 1 cut(s) 1359
Psp124BI GAGCTC 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 1013
PspFI CCCAGC 1 cut(s) 1175
PspN4I GGNNCC 1 cut(s) 769
PspPI GGNCC 3 cut(s) 456, 1013, 1168
PspPPI RGGWCCY 1 cut(s) 1013
PstNI CAGNNNCTG 1 cut(s) 278
RsaI GTAC 5 cut(s) 340, 393, 841, 1047, 1432
RsaNI GTAC 5 cut(s) 339, 392, 840, 1046, 1431
RseI CAYNNNNRTG 5 cut(s) 99, 251, 305, 423, 466
SacI GAGCTC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 7 cut(s) 20, 84, 357, 747, 879, 1532, 1591
SatI GCNGC 2 cut(s) 7, 1150
Sau3AI GATC 3 cut(s) 679, 942, 1159
Sau96I GGNCC 3 cut(s) 456, 1013, 1168
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 1 cut(s) 734
SduI GDGCHC 3 cut(s) 272, 1143, 1552
SfaNI GCATC 4 cut(s) 586, 767, 897, 1589
SinI GGWCC 1 cut(s) 1013
SmiMI CAYNNNNRTG 5 cut(s) 99, 251, 305, 423, 466
SmlI CTYRAG 3 cut(s) 83, 830, 983
SmoI CTYRAG 3 cut(s) 83, 830, 983
SpeI ACTAGT 2 cut(s) 536, 847
Sse9I AATT 9 cut(s) 213, 241, 482, 675, 692, 1068, 1316, 1434, 1478
SsiI CCGC 1 cut(s) 1226
SspMI CTAG 3 cut(s) 537, 848, 1572
SstI GAGCTC 1 cut(s) 272
TaaI ACNGT 2 cut(s) 204, 1232
TaqI TCGA 2 cut(s) 466, 1511
TasI AATT 9 cut(s) 213, 241, 482, 675, 692, 1068, 1316, 1434, 1478
TatI WGTACW 1 cut(s) 1045
TfiI GAWTC 2 cut(s) 275, 790
Tru1I TTAA 7 cut(s) 20, 84, 357, 747, 879, 1532, 1591
Tru9I TTAA 7 cut(s) 20, 84, 357, 747, 879, 1532, 1591
TscAI CASTG 4 cut(s) 142, 207, 1035, 1057
TseFI GTSAC 4 cut(s) 106, 1030, 1390, 1574
TseI GCWGC 2 cut(s) 6, 1149
Tsp45I GTSAC 4 cut(s) 106, 1030, 1390, 1574
TspRI CASTG 4 cut(s) 142, 207, 1035, 1057
Vha464I CTTAAG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 4 cut(s) 213, 482, 1068, 1478
XceI RCATGY 3 cut(s) 366, 1059, 1363
XspI CTAG 3 cut(s) 537, 848, 1572
ZrmI AGTACT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.