Rroxscaffold_6G00397530

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005202
Physical Location & Seq
Forward (+)
19216130 .. 19220909
4780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397530.1

Sequence Viewer

Length: 1824 bp
ATGCTATGTGAACTACTTTTTTGGCTCTCACGTGGGAGCTGGTATGAAATTGCCCCATCATTTAATGAAATTTCAATGCAACCAAATAGTAAGTGGGCTTCCGGATGTATTCACCGGGTCATAATAGCCCCAAGTCTTTCCCTCCCTCCCCTCGCCAACCAGTTCAATTTCATGTCATGCTGGAATTCCCTACTCTTCATTTGTTTGCTTCAGACCACTCTTCTCCAAATGAGCAGACTCGTTTTCAAATCAAACCCACACCCTCCTCCCTGTCTTCTTCTTCTTCAAACATGTTCAAGCATGAACCAACTCAAGCAAATCCATGCCCACACACTCATCACCGGCCTAGCTCGTTTCACCTACATCACCAGCAAGCTCTTAGCCTTCTCCGCCTTATCGGAACACGGTGATTTACGCCACGCAGAGACTCTATTCAATCGAATTCCGTCACCCAATGTCTTTGATTTCAATTCCATGATCATGGGTTTCTTGAAAAAATCACATTCCGAAAAGGGTCTCTCTGTTTTTACCAAAATGAGAAGGATAGGCACCGACCCAAATGCTCGAACTTTCACAGTTTTGGTTAAAGCTTGTGTTGGGGTGCCTTTGCTTGATCAGATATATGGTCAGATAATGAAGTTTGGACATGAGTGTGATGTTTATGTTACTAGCTCAGTTATTAGTGTGTACTGTAAATTGAGAGCTGTGGAATTTGCTCGGAGAGTGTTTGATGAAAGTTTGGATAGGAATGTGGTGTGTTGGACTAGTCTTGTTAGTGGGTATTGTGGCAATGGGTTGGTTGATGATGCCCGTGACCTGTTCGATAAAATGCCTGAGAGAAATGATGTGTCTTACAGTGCAATGGTTTCGGGTTATGTTCAGAATGCTTGTTTCAACAAAGCTATTGATTTGTTTCGTGATTTCAAGCGTCGTCATGCGAATGTGAGGTTGAAAGAGTCTCTTTTGGTTAGTGTTCTTAATGCCTGTGCTTCTGTTGGTGCATTCGAGGAAGGGAAGTGGATTCAGATGTATTTAGATGACCATGGTTTTGAGTATGGACTTGAGCTTGGTACGGCGGGAATAGATTTTTATGCAAAATGTGGATTCATTAAAGATGCTAAAGAGATATTTGATAAGATGCCGCATAAAGATGTAACTACTTGGAGTGCTATGATCATGGGATATGCTACTAATGGTGAGAATGATACAGGGCTTGAACTTTTTGCTGAAATGGAAAAGAAGGGACCTAAACCAAATGCTGTGACATTTGTTGGAGTTCTCACTGCATGTAATCACAAAACTCTGGTAAATGAAGCATGGCGAGTATTGGGACGTATGAATAAAATTTATAGCATTGCTCCAGAGATTGAGCACTATGGGTGCATGGTTGATCTCTTGGCCCGTGCTGGGCGAGTGAAAGAGGCAGAGATATTGATAAGAAGTATGGCAATGGAACCAGACGGAGCTATATGGGGGTCTTTACTTAACGGGTGTCTGATACATGGCCATTTTGAGTTAGCCGGTAAGGTTGGGAAGCTTCTCATTCAATTAGAGCCTCAACATAGTGGAAGGTATGTTCTTTTAGCGAACATGTATGCCAAAATGGAAAACTGGGAGGATGTGTTGAGGCTAAGGAAAATGATGAAGGATAGAGAGGTAGTTTCAATTTCTGCTTGGAGTTTCATCGAGATTGATGGTGTTGTGCATAAATTCTTAGCCAATGATAAGTCTCATTCTCATTCAAGAAGTATATACCAAACATTAAGTCAACTGAGGAAGGAACTCGAAAGTTTTTCCAATGCCAATGATTCTTTGCTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

607

Amino Acids

68.52

Weight (kDa)

8.04

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 151 - 199 3.7e-10 PPR repeat family
PPR_1 PF12854 248 - 277 3.3e-09 PPR repeat
PPR_2 PF13041 249 - 281 4.5e-07 PPR repeat family
PPR PF01535 252 - 281 9.5e-08 PPR repeat
PPR_2 PF13041 383 - 430 2.1e-10 PPR repeat family
PPR PF01535 387 - 415 1e-05 PPR repeat
E_motif PF20431 502 - 553 1.3e-10 E motif
Eplus_motif PF20430 568 - 595 4.6e-07 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 548, 601
AccIII TCCGGA 1 cut(s) 101
AciI CCGC 3 cut(s) 390, 1076, 1142
AcoI YGGCCR 1 cut(s) 1504
AcsI RAATTY 6 cut(s) 69, 184, 441, 710, 1344, 1709
AcuI CTGAAG 1 cut(s) 194
AcvI CACGTG 1 cut(s) 32
AfaI GTAC 2 cut(s) 689, 1072
AfiI CCNNNNNNNGG 1 cut(s) 1407
AflIII ACRYGT 2 cut(s) 290, 1590
AhlI ACTAGT 1 cut(s) 764
AloI GAACNNNNNNTCC 2 cut(s) 1560, 1592
Alw21I GWGCWC 1 cut(s) 1374
Alw26I GTCTC 4 cut(s) 419, 521, 963, 1734
Aor13HI TCCGGA 1 cut(s) 101
AoxI GGCC 3 cut(s) 343, 1398, 1504
ApoI RAATTY 6 cut(s) 69, 184, 441, 710, 1344, 1709
ArsI GACNNNNNNTTYG 4 cut(s) 1031, 1063, 1750, 1782
AspS9I GGNCC 2 cut(s) 1244, 1399
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 7 cut(s) 104, 331, 349, 358, 419, 441, 1208
AvaII GGWCC 1 cut(s) 1244
BalI TGGCCA 1 cut(s) 1506
BanI GGYRCC 2 cut(s) 548, 601
BarI GAAGNNNNNNTAC 2 cut(s) 82, 114
BbrPI CACGTG 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 1374
BccI CCATC 2 cut(s) 64, 1688
BceAI ACGGC 1 cut(s) 1089
BcgI CGANNNNNNTGC 4 cut(s) 542, 576, 849, 883
BclI TGATCA 3 cut(s) 477, 613, 1173
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 4 cut(s) 419, 521, 963, 1734
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 3 cut(s) 347, 669, 765
BisI GCNGC 1 cut(s) 1142
BlsI GCNGC 1 cut(s) 1143
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 1244
BmgT120I GGNCC 2 cut(s) 1244, 1399
BmiI GGNNCC 4 cut(s) 550, 603, 1245, 1455
BmrFI CCNGG 1 cut(s) 116
BmrI ACTGGG 1 cut(s) 1621
BmsI GCATC 3 cut(s) 796, 1105, 1128
BmuI ACTGGG 1 cut(s) 1621
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 1344
Bpu10I CCTNAGC 1 cut(s) 1631
BpuEI CTTGAG 2 cut(s) 296, 1082
BpuMI CCSGG 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 1 cut(s) 1042
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 1607, 1637
Bsc4I CCNNNNNNNGG 1 cut(s) 1407
Bse118I RCCGGY 2 cut(s) 341, 1520
Bse1I ACTGG 2 cut(s) 160, 1616
Bse3DI GCAATG 4 cut(s) 796, 867, 1353, 1455
BseAI TCCGGA 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 1042
BseGI GGATG 2 cut(s) 110, 1624
BseLI CCNNNNNNNGG 1 cut(s) 1407
BseMI GCAATG 4 cut(s) 796, 867, 1353, 1455
BseMII CTCAG 3 cut(s) 687, 825, 1763
BseNI ACTGG 2 cut(s) 160, 1616
BseRI GAGGAG 1 cut(s) 255
BseYI CCCAGC 1 cut(s) 1406
BshFI GGCC 3 cut(s) 345, 1400, 1506
BshNI GGYRCC 2 cut(s) 548, 601
BsiHKAI GWGCWC 1 cut(s) 1374
BsiSI CCGG 4 cut(s) 102, 115, 342, 1521
BslFI GGGAC 2 cut(s) 1257, 1344
BslI CCNNNNNNNGG 1 cut(s) 1407
BsmAI GTCTC 4 cut(s) 419, 521, 963, 1734
BsmFI GGGAC 2 cut(s) 1257, 1344
BsmI GAATGC 2 cut(s) 889, 1001
BsnI GGCC 3 cut(s) 345, 1400, 1506
Bso31I GGTCTC 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 1374
Bsp13I TCCGGA 1 cut(s) 101
Bsp143I GATC 4 cut(s) 477, 613, 1173, 1390
Bsp19I CCATGG 1 cut(s) 1042
BspACI CCGC 3 cut(s) 390, 1076, 1142
BspANI GGCC 3 cut(s) 345, 1400, 1506
BspCNI CTCAG 3 cut(s) 686, 826, 1764
BspEI TCCGGA 1 cut(s) 101
BspLI GGNNCC 4 cut(s) 550, 603, 1245, 1455
BspT107I GGYRCC 2 cut(s) 548, 601
BspTNI GGTCTC 1 cut(s) 521
BsrDI GCAATG 4 cut(s) 796, 867, 1353, 1455
BsrFI RCCGGY 2 cut(s) 341, 1520
BsrI ACTGG 2 cut(s) 160, 1616
BssAI RCCGGY 2 cut(s) 341, 1520
BssECI CCNNGG 1 cut(s) 1042
BssMI GATC 4 cut(s) 477, 613, 1173, 1390
BssT1I CCWWGG 1 cut(s) 1042
Bst4CI ACNGT 4 cut(s) 407, 577, 692, 857
Bst6I CTCTTC 2 cut(s) 200, 225
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 374
BstDEI CTNAG 6 cut(s) 379, 673, 834, 1631, 1714, 1772
BstDSI CCRYGG 1 cut(s) 1042
BstF5I GGATG 2 cut(s) 110, 1624
BstKTI GATC 4 cut(s) 480, 616, 1176, 1393
BstMAI GTCTC 4 cut(s) 419, 521, 963, 1734
BstMBI GATC 4 cut(s) 477, 613, 1173, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNSI RCATGY 3 cut(s) 294, 1290, 1594
BstSCI CCNGG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 345, 1400, 1506
BtgI CCRYGG 1 cut(s) 1042
BtsCI GGATG 2 cut(s) 110, 1624
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 2 cut(s) 862, 1281
Cac8I GCNNGC 1 cut(s) 374
Cfr10I RCCGGY 2 cut(s) 341, 1520
Cfr13I GGNCC 2 cut(s) 1244, 1399
CseI GACGC 1 cut(s) 917
Csp6I GTAC 2 cut(s) 688, 1071
CviQI GTAC 2 cut(s) 688, 1071
DdeI CTNAG 6 cut(s) 379, 673, 834, 1631, 1714, 1772
DpnI GATC 4 cut(s) 479, 615, 1175, 1392
DpnII GATC 4 cut(s) 477, 613, 1173, 1390
EaeI YGGCCR 1 cut(s) 1504
Eam1104I CTCTTC 2 cut(s) 200, 225
EarI CTCTTC 2 cut(s) 200, 225
EciI GGCGGA 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 1042
Eco31I GGTCTC 1 cut(s) 521
Eco47I GGWCC 1 cut(s) 1244
Eco57I CTGAAG 1 cut(s) 194
Eco72I CACGTG 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRI GAATTC 2 cut(s) 184, 441
EcoT14I CCWWGG 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1042
FalI AAGNNNNNCTT 2 cut(s) 945, 977
FaqI GGGAC 2 cut(s) 1257, 1344
FauI CCCGC 1 cut(s) 1069
FbaI TGATCA 3 cut(s) 477, 613, 1173
Fnu4HI GCNGC 1 cut(s) 1142
FokI GGATG 2 cut(s) 117, 1631
Fsp4HI GCNGC 1 cut(s) 1142
FspBI CTAG 3 cut(s) 347, 669, 765
GluI GCNGC 1 cut(s) 1142
GsaI CCCAGC 1 cut(s) 1410
GsuI CTGGAG 1 cut(s) 1344
HaeIII GGCC 3 cut(s) 345, 1400, 1506
HapII CCGG 4 cut(s) 102, 115, 342, 1521
HgaI GACGC 1 cut(s) 917
HincII GTYRAC 1 cut(s) 1769
HindII GTYRAC 1 cut(s) 1769
HindIII AAGCTT 2 cut(s) 588, 1535
HinfI GANTC 6 cut(s) 237, 427, 956, 1021, 1104, 1808
HpaII CCGG 4 cut(s) 102, 115, 342, 1521
HphI GGTGA 7 cut(s) 104, 331, 349, 358, 419, 441, 1208
Hpy166II GTNNAC 3 cut(s) 11, 688, 1769
Hpy188I TCNGA 9 cut(s) 213, 400, 508, 618, 630, 720, 882, 1026, 1497
Hpy188III TCNNGA 6 cut(s) 102, 490, 917, 1361, 1687, 1743
Hpy8I GTNNAC 3 cut(s) 11, 688, 1769
Hpy99I CGWCG 1 cut(s) 933
HpyAV CCTTC 7 cut(s) 394, 534, 1004, 1234, 1563, 1639, 1771
HpyCH4III ACNGT 4 cut(s) 407, 577, 692, 857
HpyCH4IV ACGT 2 cut(s) 31, 1333
HpyCH4V TGCA 7 cut(s) 79, 860, 1001, 1094, 1286, 1383, 1705
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 6 cut(s) 379, 673, 834, 1631, 1714, 1772
HpySE526I ACGT 2 cut(s) 31, 1333
Kpn2I TCCGGA 1 cut(s) 101
Ksp22I TGATCA 3 cut(s) 477, 613, 1173
Kzo9I GATC 4 cut(s) 477, 613, 1173, 1390
LmnI GCTCC 3 cut(s) 36, 1363, 1463
LweI GCATC 3 cut(s) 796, 1105, 1128
MaeI CTAG 3 cut(s) 347, 669, 765
MaeII ACGT 2 cut(s) 31, 1333
MaeIII GTNAC 5 cut(s) 447, 664, 812, 1153, 1261
MalI GATC 4 cut(s) 479, 615, 1175, 1392
MboI GATC 4 cut(s) 477, 613, 1173, 1390
MboII GAAGA 6 cut(s) 187, 212, 266, 269, 272, 275
MhlI GDGCHC 1 cut(s) 1374
MlsI TGGCCA 1 cut(s) 1506
MluNI TGGCCA 1 cut(s) 1506
MlyI GAGTC 3 cut(s) 231, 421, 965
MmeI TCCRAC 2 cut(s) 740, 1252
Mox20I TGGCCA 1 cut(s) 1506
MroI TCCGGA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 1506
MseI TTAA 6 cut(s) 63, 585, 978, 1110, 1485, 1763
MslI CAYNNNNRTG 4 cut(s) 479, 651, 939, 1149
Msp20I TGGCCA 1 cut(s) 1506
MspI CCGG 4 cut(s) 102, 115, 342, 1521
MspR9I CCNGG 1 cut(s) 116
Mva1269I GAATGC 2 cut(s) 889, 1001
MwoI GCNNNNNNNGC 1 cut(s) 389
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 1042
NdeII GATC 4 cut(s) 477, 613, 1173, 1390
NlaIV GGNNCC 4 cut(s) 550, 603, 1245, 1455
NmuCI GTSAC 3 cut(s) 447, 812, 1261
NspI RCATGY 3 cut(s) 294, 1290, 1594
PciI ACATGT 2 cut(s) 290, 1590
PctI GAATGC 2 cut(s) 889, 1001
PfeI GAWTC 3 cut(s) 1021, 1104, 1808
PkrI GCNGC 1 cut(s) 1143
PleI GAGTC 3 cut(s) 231, 421, 964
PmaCI CACGTG 1 cut(s) 32
PmlI CACGTG 1 cut(s) 32
PpsI GAGTC 3 cut(s) 231, 421, 964
Ppu21I YACGTR 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 1244
PscI ACATGT 2 cut(s) 290, 1590
Psp5II RGGWCCY 1 cut(s) 1244
PspCI CACGTG 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 1406
PspN4I GGNNCC 4 cut(s) 550, 603, 1245, 1455
PspPI GGNCC 2 cut(s) 1244, 1399
PspPPI RGGWCCY 1 cut(s) 1244
RsaI GTAC 2 cut(s) 689, 1072
RsaNI GTAC 2 cut(s) 688, 1071
RseI CAYNNNNRTG 4 cut(s) 479, 651, 939, 1149
SaqAI TTAA 6 cut(s) 63, 585, 978, 1110, 1485, 1763
SatI GCNGC 1 cut(s) 1142
Sau3AI GATC 4 cut(s) 477, 613, 1173, 1390
Sau96I GGNCC 2 cut(s) 1244, 1399
SchI GAGTC 3 cut(s) 231, 421, 965
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 1374
SfaNI GCATC 3 cut(s) 796, 1105, 1128
SinI GGWCC 1 cut(s) 1244
SmiMI CAYNNNNRTG 4 cut(s) 479, 651, 939, 1149
SmlI CTYRAG 2 cut(s) 311, 1061
SmoI CTYRAG 2 cut(s) 311, 1061
SpeI ACTAGT 1 cut(s) 764
SsiI CCGC 3 cut(s) 390, 1076, 1142
SspMI CTAG 3 cut(s) 347, 669, 765
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 1042
TaaI ACNGT 4 cut(s) 407, 577, 692, 857
TaiI ACGT 2 cut(s) 34, 1336
TaqI TCGA 6 cut(s) 439, 565, 822, 1005, 1686, 1785
TatI WGTACW 1 cut(s) 687
TauI GCSGC 1 cut(s) 1144
TfiI GAWTC 3 cut(s) 1021, 1104, 1808
Tru1I TTAA 6 cut(s) 63, 585, 978, 1110, 1485, 1763
Tru9I TTAA 6 cut(s) 63, 585, 978, 1110, 1485, 1763
TscAI CASTG 2 cut(s) 862, 1288
TseFI GTSAC 3 cut(s) 447, 812, 1261
Tsp45I GTSAC 3 cut(s) 447, 812, 1261
TspGWI ACGGA 2 cut(s) 435, 1476
TspRI CASTG 2 cut(s) 862, 1288
VpaK11BI GGWCC 1 cut(s) 1244
XapI RAATTY 6 cut(s) 69, 184, 441, 710, 1344, 1709
XceI RCATGY 3 cut(s) 294, 1290, 1594
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XspI CTAG 3 cut(s) 347, 669, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.