RLG00000026276

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005202
Physical Location & Seq
Forward (+)
1831831 .. 1833354
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026276

Sequence Viewer

Length: 1524 bp
ATGAACCAACTCAAGCAAATCCATGCCCACACACTCATCACCGGCCTTGTTCGTTTCACCTACATCACCAGCAAGCTCTTAGCCTTCTCCGCCTTATCGGAACACGGTGATTTACACCACGCAGAGACTCTATTCAATCGAATTCCGGCACCCAATGTCTTTGATTTCAATTCCATGATCATGGGTTTCTTGAAAGACTCACATTCCGAAAAGGGTCTCTCTGTTTTTACCAAAATGAGAAGGATAGGCGCCGACCCAAATGCTCGAACTTTCACAGTTTTGGTTAAAGCTTGTGTTGGGGTGCCTTTGCTTGATCAGATATATGGTCAGATAATGAAGTTTGGACATGAGTGTGATGTTTATGTTACTAGCTCAGTTATTAGTGTGTACTGTAAATTGAGAGCTGTGGAATTTGCTCGGAGAGTGTTTGATGAAAGTTTGGATAGGAATGTGGTGTGTTGGACTAGTCTTGTTAGTGGGTATTGTGGCAATGGGTTGGTTGATGATGCCCGTGACCTGTTCGATAAAATGCCTGAGAGAAATGATGTGTCTTACAGTGCAATGGTTTCGGGTTATGTTCAGAATGCTTGGTTCAACAAAGCTATTGATTTGTTTCGTGATTTCAAGCTTCGTCGTGCGAATGTGAGGTTGAAAGAGTCTCTTTTGGTTAGTGTTCTTAATGCCTGTGCTTCTGTGGGTGCATTCGAGGAAGGGAAGTGGATTCAGATGTATTTGGATGGCCAGGGTTTTGAGTATGGACTTGAGCTCGGTACGGCAGGAATAGATTTTTATGCAAAATGTGGATTCATTAAAGATGCTAAAGAGATGTTTGATAAGATGCCGCGTAAAGATGTAACTACTTGGAGTGCTATGATCATGGGATATGCTACTAATGGTGAGAATGATACAGGGCTTGAACTTTTTGCTGAAATGGAAAAGAAGGGACCTAAACCAAATGCTGTGACATTTGTTGGAGTTCTCACTGCATGTAATCACAAAACTCTGGTAAATGAAGCATGGCGAGTATTGGGACGTATGAGTAAAATTTATAGCATTGCTCCAGAGATTGAGCACTATGGGTGCATGGTTGATCTCTTGGCCCGTGCTGGGCGAGTGAAAGAGGCAGAGATATTGATAAGAAGTATGGCAATGGAACCAGACGGAGCTATATGGGGGTCTTTACTTAACGGGTGTCTGATACACGGCCATTTTGAGTTAGCCGGTAAGGTTGGGAAGCTTCTCATTCAATTAGAGCCTCAACATAGTGGAAGGTATGTTCTTTTAGCGAACATGTATGCCAAAATGGAAAACTGGGAGGATGTGTTGAGGCTAAGGAAAATGATGAAGGGTAGAGAGGTAGTTTCAATTTCTGCTTGGAGTTTCATCGAGATTGATGGTGTTGTGCATAAATTCTTAGCCAATGATAAGTCTCATTCTCATTCAAGAAGTATATACCAAACATTAAGTCAACTGAGGAAGGAACTCGAAAGTTTTTCCAATGCCAATGATTCTTTGCTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

508

Amino Acids

57.06

Weight (kDa)

7.6

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 51 - 99 1.2e-09 PPR repeat family
PPR_1 PF12854 148 - 177 2.7e-09 PPR repeat
PPR_2 PF13041 149 - 181 3.6e-07 PPR repeat family
PPR PF01535 152 - 181 7.6e-08 PPR repeat
PPR_2 PF13041 283 - 330 1.7e-10 PPR repeat family
PPR PF01535 287 - 315 8.1e-06 PPR repeat
E_motif PF20431 402 - 453 1.9e-10 E motif
Eplus_motif PF20430 468 - 495 3.7e-07 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 148, 248, 301
AccII CGCG 1 cut(s) 844
AciI CCGC 2 cut(s) 90, 842
AcoI YGGCCR 2 cut(s) 739, 1204
AcsI RAATTY 4 cut(s) 141, 410, 1044, 1409
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 2 cut(s) 389, 772
AfiI CCNNNNNNNGG 1 cut(s) 1107
AflIII ACRYGT 1 cut(s) 1290
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 1 cut(s) 741
AloI GAACNNNNNNTCC 2 cut(s) 1260, 1292
AluBI AGCT 9 cut(s) 76, 290, 372, 404, 602, 628, 766, 1166, 1237
AluI AGCT 9 cut(s) 76, 290, 372, 404, 602, 628, 766, 1166, 1237
Alw21I GWGCWC 2 cut(s) 768, 1074
Alw26I GTCTC 4 cut(s) 119, 221, 663, 1434
AoxI GGCC 4 cut(s) 43, 739, 1098, 1204
ApoI RAATTY 4 cut(s) 141, 410, 1044, 1409
ArsI GACNNNNNNTTYG 2 cut(s) 1450, 1482
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 2 cut(s) 944, 1099
AsuHPI GGTGA 5 cut(s) 31, 49, 58, 119, 908
AvaII GGWCC 1 cut(s) 944
BalI TGGCCA 1 cut(s) 741
BanI GGYRCC 3 cut(s) 148, 248, 301
BanII GRGCYC 1 cut(s) 768
Bbv12I GWGCWC 2 cut(s) 768, 1074
BccI CCATC 2 cut(s) 731, 1388
BceAI ACGGC 2 cut(s) 789, 1219
BcgI CGANNNNNNTGC 4 cut(s) 242, 276, 549, 583
BciT130I CCWGG 1 cut(s) 743
BclI TGATCA 3 cut(s) 177, 313, 873
BcoDI GTCTC 4 cut(s) 119, 221, 663, 1434
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 2 cut(s) 369, 465
BfoI RGCGCY 1 cut(s) 252
BisI GCNGC 1 cut(s) 842
BlsI GCNGC 1 cut(s) 843
Bme1390I CCNGG 1 cut(s) 743
Bme18I GGWCC 1 cut(s) 944
BmgT120I GGNCC 2 cut(s) 944, 1099
BmiI GGNNCC 5 cut(s) 150, 250, 303, 945, 1155
BmrFI CCNGG 1 cut(s) 743
BmrI ACTGGG 1 cut(s) 1321
BmsI GCATC 3 cut(s) 496, 805, 828
BmuI ACTGGG 1 cut(s) 1321
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 1331
BpuEI CTTGAG 1 cut(s) 782
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 742
BsaXI ACNNNNNCTCC 2 cut(s) 1307, 1337
Bsc4I CCNNNNNNNGG 1 cut(s) 1107
Bse118I RCCGGY 2 cut(s) 41, 1220
Bse1I ACTGG 1 cut(s) 1316
Bse3DI GCAATG 4 cut(s) 496, 567, 1053, 1155
BseBI CCWGG 1 cut(s) 743
BseDI CCNNGG 1 cut(s) 742
BseGI GGATG 2 cut(s) 742, 1324
BseLI CCNNNNNNNGG 1 cut(s) 1107
BseMI GCAATG 4 cut(s) 496, 567, 1053, 1155
BseMII CTCAG 3 cut(s) 387, 525, 1463
BseNI ACTGG 1 cut(s) 1316
BseYI CCCAGC 1 cut(s) 1106
Bsh1236I CGCG 1 cut(s) 844
BshFI GGCC 4 cut(s) 45, 741, 1100, 1206
BshNI GGYRCC 3 cut(s) 148, 248, 301
BsiHKAI GWGCWC 2 cut(s) 768, 1074
BsiSI CCGG 3 cut(s) 42, 146, 1221
BslFI GGGAC 2 cut(s) 957, 1044
BslI CCNNNNNNNGG 1 cut(s) 1107
BsmAI GTCTC 4 cut(s) 119, 221, 663, 1434
BsmFI GGGAC 2 cut(s) 957, 1044
BsmI GAATGC 2 cut(s) 589, 701
BsnI GGCC 4 cut(s) 45, 741, 1100, 1206
Bso31I GGTCTC 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 768, 1074
Bsp143I GATC 4 cut(s) 177, 313, 873, 1090
BspACI CCGC 2 cut(s) 90, 842
BspANI GGCC 4 cut(s) 45, 741, 1100, 1206
BspCNI CTCAG 3 cut(s) 386, 526, 1464
BspFNI CGCG 1 cut(s) 844
BspLI GGNNCC 5 cut(s) 150, 250, 303, 945, 1155
BspT107I GGYRCC 3 cut(s) 148, 248, 301
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 4 cut(s) 496, 567, 1053, 1155
BsrFI RCCGGY 2 cut(s) 41, 1220
BsrI ACTGG 1 cut(s) 1316
BssAI RCCGGY 2 cut(s) 41, 1220
BssECI CCNNGG 1 cut(s) 742
BssMI GATC 4 cut(s) 177, 313, 873, 1090
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 743
Bst4CI ACNGT 4 cut(s) 107, 277, 392, 557
BstACI GRCGYC 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 6 cut(s) 79, 373, 534, 1331, 1414, 1472
BstF5I GGATG 2 cut(s) 742, 1324
BstFNI CGCG 1 cut(s) 844
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 4 cut(s) 180, 316, 876, 1093
BstMAI GTCTC 4 cut(s) 119, 221, 663, 1434
BstMBI GATC 4 cut(s) 177, 313, 873, 1090
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 743
BstNSI RCATGY 2 cut(s) 990, 1294
BstSCI CCNGG 1 cut(s) 741
BstUI CGCG 1 cut(s) 844
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuRI GGCC 4 cut(s) 45, 741, 1100, 1206
BtsCI GGATG 2 cut(s) 742, 1324
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 2 cut(s) 562, 981
Cac8I GCNNGC 1 cut(s) 74
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 2 cut(s) 41, 1220
Cfr13I GGNCC 2 cut(s) 944, 1099
Csp6I GTAC 2 cut(s) 388, 771
CviAII CATG 9 cut(s) 23, 175, 181, 347, 877, 987, 1017, 1084, 1291
CviQI GTAC 2 cut(s) 388, 771
DdeI CTNAG 6 cut(s) 79, 373, 534, 1331, 1414, 1472
DinI GGCGCC 1 cut(s) 250
DpnI GATC 4 cut(s) 179, 315, 875, 1092
DpnII GATC 4 cut(s) 177, 313, 873, 1090
EaeI YGGCCR 2 cut(s) 739, 1204
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 766
Eco24I GRGCYC 1 cut(s) 768
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 944
Eco53kI GAGCTC 1 cut(s) 766
EcoICRI GAGCTC 1 cut(s) 766
EcoO109I RGGNCCY 1 cut(s) 944
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 741
EcoT38I GRGCYC 1 cut(s) 768
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
FaeI CATG 9 cut(s) 26, 178, 184, 350, 880, 990, 1020, 1087, 1294
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FaqI GGGAC 2 cut(s) 957, 1044
FatI CATG 9 cut(s) 22, 174, 180, 346, 876, 986, 1016, 1083, 1290
FbaI TGATCA 3 cut(s) 177, 313, 873
Fnu4HI GCNGC 1 cut(s) 842
FokI GGATG 2 cut(s) 749, 1331
FriOI GRGCYC 1 cut(s) 768
Fsp4HI GCNGC 1 cut(s) 842
FspBI CTAG 2 cut(s) 369, 465
GlaI GCGC 1 cut(s) 250
GluI GCNGC 1 cut(s) 842
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 1 cut(s) 1044
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 4 cut(s) 45, 741, 1100, 1206
HapII CCGG 3 cut(s) 42, 146, 1221
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 9 cut(s) 26, 178, 184, 350, 880, 990, 1020, 1087, 1294
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 1469
HindII GTYRAC 1 cut(s) 1469
HindIII AAGCTT 3 cut(s) 288, 626, 1235
HinfI GANTC 6 cut(s) 127, 197, 656, 721, 804, 1508
HpaII CCGG 3 cut(s) 42, 146, 1221
HphI GGTGA 5 cut(s) 31, 49, 58, 119, 908
Hpy166II GTNNAC 2 cut(s) 388, 1469
Hpy188I TCNGA 8 cut(s) 100, 208, 318, 330, 420, 582, 726, 1197
Hpy188III TCNNGA 5 cut(s) 190, 617, 1061, 1387, 1443
Hpy8I GTNNAC 2 cut(s) 388, 1469
Hpy99I CGWCG 1 cut(s) 636
HpyAV CCTTC 7 cut(s) 94, 234, 704, 934, 1263, 1339, 1471
HpyCH4III ACNGT 4 cut(s) 107, 277, 392, 557
HpyCH4IV ACGT 1 cut(s) 1033
HpyCH4V TGCA 6 cut(s) 560, 701, 794, 986, 1083, 1405
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 6 cut(s) 79, 373, 534, 1331, 1414, 1472
HpySE526I ACGT 1 cut(s) 1033
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 9 cut(s) 26, 178, 184, 350, 880, 990, 1020, 1087, 1294
HspAI GCGC 1 cut(s) 249
KasI GGCGCC 1 cut(s) 248
Ksp22I TGATCA 3 cut(s) 177, 313, 873
Kzo9I GATC 4 cut(s) 177, 313, 873, 1090
LmnI GCTCC 2 cut(s) 1063, 1163
LweI GCATC 3 cut(s) 496, 805, 828
MaeI CTAG 2 cut(s) 369, 465
MaeII ACGT 1 cut(s) 1033
MaeIII GTNAC 4 cut(s) 364, 512, 853, 961
MalI GATC 4 cut(s) 179, 315, 875, 1092
MboI GATC 4 cut(s) 177, 313, 873, 1090
MhlI GDGCHC 2 cut(s) 768, 1074
MlsI TGGCCA 1 cut(s) 741
MluCI AATT 8 cut(s) 141, 169, 395, 410, 1044, 1247, 1365, 1409
MluNI TGGCCA 1 cut(s) 741
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 3 cut(s) 121, 191, 665
MmeI TCCRAC 2 cut(s) 440, 952
MnlI CCTC 8 cut(s) 639, 700, 1114, 1266, 1309, 1320, 1348, 1467
Mox20I TGGCCA 1 cut(s) 741
MscI TGGCCA 1 cut(s) 741
MseI TTAA 5 cut(s) 285, 678, 810, 1185, 1463
MslI CAYNNNNRTG 2 cut(s) 179, 351
Msp20I TGGCCA 1 cut(s) 741
MspI CCGG 3 cut(s) 42, 146, 1221
MspR9I CCNGG 1 cut(s) 743
Mva1269I GAATGC 2 cut(s) 589, 701
MvaI CCWGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 844
MwoI GCNNNNNNNGC 1 cut(s) 89
NarI GGCGCC 1 cut(s) 249
NdeII GATC 4 cut(s) 177, 313, 873, 1090
NlaIII CATG 9 cut(s) 26, 178, 184, 350, 880, 990, 1020, 1087, 1294
NlaIV GGNNCC 5 cut(s) 150, 250, 303, 945, 1155
NmuCI GTSAC 2 cut(s) 512, 961
NspI RCATGY 2 cut(s) 990, 1294
PciI ACATGT 1 cut(s) 1290
PctI GAATGC 2 cut(s) 589, 701
PfeI GAWTC 3 cut(s) 721, 804, 1508
PkrI GCNGC 1 cut(s) 843
PleI GAGTC 3 cut(s) 121, 191, 664
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 3 cut(s) 121, 191, 664
PpuMI RGGWCCY 1 cut(s) 944
PscI ACATGT 1 cut(s) 1290
Psp124BI GAGCTC 1 cut(s) 768
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 1 cut(s) 741
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 1 cut(s) 741
PspN4I GGNNCC 5 cut(s) 150, 250, 303, 945, 1155
PspPI GGNCC 2 cut(s) 944, 1099
PspPPI RGGWCCY 1 cut(s) 944
RsaI GTAC 2 cut(s) 389, 772
RsaNI GTAC 2 cut(s) 388, 771
RseI CAYNNNNRTG 2 cut(s) 179, 351
SacI GAGCTC 1 cut(s) 768
SaqAI TTAA 5 cut(s) 285, 678, 810, 1185, 1463
SatI GCNGC 1 cut(s) 842
Sau3AI GATC 4 cut(s) 177, 313, 873, 1090
Sau96I GGNCC 2 cut(s) 944, 1099
SchI GAGTC 3 cut(s) 121, 191, 665
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 2 cut(s) 768, 1074
SfaNI GCATC 3 cut(s) 496, 805, 828
SfoI GGCGCC 1 cut(s) 250
SinI GGWCC 1 cut(s) 944
SmiMI CAYNNNNRTG 2 cut(s) 179, 351
SmlI CTYRAG 2 cut(s) 11, 761
SmoI CTYRAG 2 cut(s) 11, 761
SpeI ACTAGT 1 cut(s) 464
Sse9I AATT 8 cut(s) 141, 169, 395, 410, 1044, 1247, 1365, 1409
SsiI CCGC 2 cut(s) 90, 842
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 2 cut(s) 369, 465
SstI GAGCTC 1 cut(s) 768
StyD4I CCNGG 1 cut(s) 741
TaaI ACNGT 4 cut(s) 107, 277, 392, 557
TaiI ACGT 1 cut(s) 1036
TaqI TCGA 6 cut(s) 139, 265, 522, 705, 1386, 1485
TasI AATT 8 cut(s) 141, 169, 395, 410, 1044, 1247, 1365, 1409
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 3 cut(s) 721, 804, 1508
Tru1I TTAA 5 cut(s) 285, 678, 810, 1185, 1463
Tru9I TTAA 5 cut(s) 285, 678, 810, 1185, 1463
TscAI CASTG 2 cut(s) 562, 988
TseFI GTSAC 2 cut(s) 512, 961
Tsp45I GTSAC 2 cut(s) 512, 961
TspDTI ATGAA 7 cut(s) 17, 350, 447, 796, 1026, 1358, 1372
TspGWI ACGGA 1 cut(s) 1176
TspRI CASTG 2 cut(s) 562, 988
VpaK11BI GGWCC 1 cut(s) 944
XapI RAATTY 4 cut(s) 141, 410, 1044, 1409
XceI RCATGY 2 cut(s) 990, 1294
XspI CTAG 2 cut(s) 369, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.