Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006287

Physical Location & Seq

Genomic Coordinates Reverse (-)

28458070 .. 28461704

Gene Structure 3635 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1196300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2526 bp

ATGGAGATTCTTGGTGGGTTTTTGGTCATGGATTTTGGTATGGTAAAGGGAGGGAAGGTTAGTTCCAAGCGAAAAATCAAGACAAGGGTTCCGTCAAAGTATCAGGGTTCCGATGATTCGGATGAGGATTATGTGATTTCGGGTGAAGAAAATGAAGTTTCTGAGGATTTAGAGGAGGATTACTGTTCTTCTTTGGATGGAGATGCGTCGGAAGAGAGCTTTGGGAGTTTTGTGGATGAGGAGGAGGAAGAGGAGGACGAAGTGAAAAAGGTGAGGAAGAAAACTCGATCAAGAGCGAAAAGGGATGCTGATGGTTTTATTGTGGATGAGGAGGAGGAGGAGGAAGAAGATGAAGAGGAAGTGAAACCGGTTAGGAAGGGAATTAGGTCAAAGGTCCAAAATGGTTTTTCTGGCCGGCCGAAAAATGGGGTTAAACCGTCAAGAAAGAGGAGAAAGATTACATATCGTGAGGTGGAGGATCAGGATTATAACGATGAAGATGATGATGACGATGAGGAATTCACACCAGATGAAGATGAATTTTCTGACGTGGAAGAAAAACCAGTGGTGAAAAAGAAGAAAGCTGATGGGAAAGTGGGCAAGCGGTGCTTGAAGAAAAAGGGTCGTCCTGTAAGAGGTGTAAAAAGGAGAAAACCTTTGGCTTCAAAGAAGCCTTTGAGAAAGAAAGGTAGAACAAGTCAGGCATCGAGGAGGAAAGCAAGAAGTGACGATGATATTGAAAGTGATAGCGATTTCATGGATAGTATTGTGCCCGTGAGAGTAACGAATGAGAAAAAAACGGTTAGGAAAAGGAGGAGAATGTCTATACCGTCAGATTCAGATTTTGTGTCTTCTGGAGCATCTGATTATGACTACACCATCTCCGAGGAAGAGAGAGAGCAAGTACGAGAAGCCAAGGAGTTGTGTGAAAGATTGGAAACTAATTTGCGAAGTTCATCAGTGCCTGCTAGCATTCAGGAGGATACACCTGTACAACAGCCAAGAAACCCTCCTAGAAGGAAGGGCAAAGAGAAGATTGAGCAAGTGAAGGTTGAGGTTGTAAAGCAGGTCTGTGGCATTTGTCTCTCCGAGGAAGATAAAAGAAGAGTGAGGGGAACACTAGATTGTTGCACTCATTATTTTTGTTTTGCCTGCATCATGGAATGGGGAAAAGTGGAATCTCGTTGCCCTTTGTGCAAGCAGAGATTTAAGACAATTAGTAAGCCTGCAAGATGTACGACAGGAATTGATTTGAGAGAAGTGGTGATACAGGTCTCTGAGCGTGATCAGGTATATCAGCCGACAGAAGAAGAACTCAGGAGTTATCTTGACCCTTACGAGAATGTGATATGTACAGAATGCCACGAAGGAGGGGAAGATGGCCTCATGTTACTGTGTGATCTATGTGATTCACCTGCTCACACCTTTTGTGTTGGTCTTGGCCGAGAAGTACCTGAAGGTAATTGGTACTGTGAGGGTTGTAGACCTGTGGCTATGGGATCCTCAAGTTCACAGGCTCAGGGTCGGTTGTCTGATCAAAGAACTACAAACAACATTTTGTCGAATAGACCATCACCTACTGTACATTTTGACGAAGGAATAGACCTTAATGTGGTGTCCTCACCTCGTCCACAATTTACTCAAGGATTACCCCCTCGATTTCCTGTTGGAGGCTTTCAAGCACCTTCCCCGGTATCTGGGGCAGGAGCACCAACTCTATCAGGGAGACGCTTAATACAGCGTTATATTCAACAACTCCTTAATTCTAATAGGACGAGTTATATGGCCAGTATAAATGAGAGGAACTCATCTGATAATTTGAGTAATGATTTCTCTCACTCTCAAGCTGCTCAGACGAGGGAGATGACAGTCCAACATACCAGGACAGACGAGATGGAGGCATCTTACCACACATTCTTTGAGGAGAGATTACAAAACAATCCTTTAGCACTAGTGCCAGATAACTTGAGTACTGATTTCTTAAATACATCAGCATCAACCCCTCAGAGAAGGCAAGCAATCGAGGATCCAACTACAACATCTGCTGATAGATCTCAAAATGGAGCATTATGGCCTGTACTTACCGGAATGAATTCAGTACCAAATTCCGGACAATTCCATCACTTCAGCGGTAGACCAAATATTAGTGGATCTGATGCTGGTGTGTCCCCTAATACTGCTACGGAACAGGGCGTTTTTCAGCTAGCAAAGGAACAACTGAAAGTGATGGTCAGAAACCACTTGGATAATTTGTCCCGAGACGTTGATCTAGACAAAGACACTTTCAAGGACATTGCAAGGAGATCTATGCACACTGTCTTGGCTGCGTGCGGACGTGAGCACAGAAGCTGTGAGGTAGTTCCCGTGCCTCCTCCCTCAACCTGCGCCCACATTGAATTAACGGTGGGTGGAATGACAAGTCTGATGAGAGGCCATTGTCCATCTTGTTTCGAATCTTTTGTAGGAGAAGTTGTAAAGAGGATCTTCGATACAAAACTCCCGCAGTGGTTGAGTTTACGTCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008623 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016590 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019899 GO:0019904 GO:0031010 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070063 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD zf-RING_2

Protein Analysis

842

Amino Acids

94.76

Weight (kDa)

5.55

Isoelectric Point (pI)

64.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	357 - 400	1.4e-06	Ring finger domain
PHD	PF00628	451 - 494	1.4e-07	PHD-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006287)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05670 AT3G05670 AT3G05670
fragaria_vesca	FvH4_6g07080
malus_domestica	MD04G1196300.v1.1 MD12G1209300.v1.1
prunus_persica	Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1
pyrus_communis	pycom04g17360 pycom12g19660
rosa_chinensis	RchiOBHm_Chr3g0456661
rosa_laevigata	RLG00000025286
rosa_multiflora	Rmu_co8126688.1_g000001 Rmu_sc0000133.1_g000031
rosa_roxburghii	Rroxscaffold_11G00450420 Rroxscaffold_6G00422560
rosa_rugosa	Rorug03G0014500 Rorug03G0014600 Rorug03G0014700 Rorug03G0014800.1
rosa_samantha	Rh3AG075300 Rh3BG077200 Rh3CG076900 Rh3DG078900
rosa_wichuraiana	Rw3G006450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	489
AarI	CACCTGC	1 cut(s)	1423
Acc36I	ACCTGC	3 cut(s)	1057, 1423, 2390
AccI	GTMKAC	2 cut(s)	1483, 2134
AccIII	TCCGGA	1 cut(s)	2108
AciI	CCGC	4 cut(s)	604, 2130, 2331, 2501
AclWI	GGATC	7 cut(s)	486, 1494, 1507, 2021, 2034, 2158, 2489
AcoI	YGGCCR	4 cut(s)	412, 416, 1441, 1785
AcsI	RAATTY	4 cut(s)	518, 539, 2092, 2104
AcuI	CTGAAG	2 cut(s)	1476, 2110
AfiI	CCNNNNNNNGG	6 cut(s)	425, 635, 1612, 1670, 1697, 2108
AgeI	ACCGGT	1 cut(s)	367
AgsI	TTSAA	7 cut(s)	613, 666, 740, 1679, 1751, 2287, 2396
AhlI	ACTAGT	1 cut(s)	1951
AjiI	CACGTC	2 cut(s)	550, 2336
AjnI	CCWGG	1 cut(s)	1880
AjuI	GAANNNNNNNTTGG	2 cut(s)	204, 236
AloI	GAACNNNNNNTCC	2 cut(s)	46, 78
AluBI	AGCT	5 cut(s)	219, 584, 1847, 2203, 2349
AluI	AGCT	5 cut(s)	219, 584, 1847, 2203, 2349
Alw21I	GWGCWC	2 cut(s)	1711, 2343
Alw26I	GTCTC	4 cut(s)	1088, 1279, 1720, 2253
AlwI	GGATC	7 cut(s)	486, 1494, 1507, 2021, 2034, 2158, 2489
AlwNI	CAGNNNCTG	2 cut(s)	965, 2349
Ama87I	CYCGRG	1 cut(s)	2256
Aor13HI	TCCGGA	1 cut(s)	2108
AoxI	GGCC	7 cut(s)	412, 416, 1381, 1441, 1785, 2072, 2431
ApeKI	GCWGC	2 cut(s)	1847, 2324
ApoI	RAATTY	4 cut(s)	518, 539, 2092, 2104
AsiGI	ACCGGT	1 cut(s)	367
Asp700I	GAANNNNTTC	1 cut(s)	2092
AspLEI	GCGC	1 cut(s)	2387
AspS9I	GGNCC	1 cut(s)	394
AsuC2I	CCSGG	1 cut(s)	1691
AsuHPI	GGTGA	7 cut(s)	155, 283, 580, 1276, 1404, 1566, 1614
AsuII	TTCGAA	1 cut(s)	2451
AsuNHI	GCTAGC	2 cut(s)	968, 2203
AvaI	CYCGRG	1 cut(s)	2256
AvaII	GGWCC	1 cut(s)	394
BaeGI	GKGCMC	1 cut(s)	774
BaeI	ACNNNNGTAYC	2 cut(s)	975, 1008
BalI	TGGCCA	1 cut(s)	1787
BamHI	GGATCC	2 cut(s)	1499, 2026
BarI	GAAGNNNNNNTAC	2 cut(s)	1251, 1283
BbsI	GAAGAC	1 cut(s)	843
Bbv12I	GWGCWC	2 cut(s)	1711, 2343
BbvI	GCAGC	2 cut(s)	1834, 2311
BciT130I	CCWGG	1 cut(s)	1882
BciVI	GTATCC	1 cut(s)	976
BclI	TGATCA	2 cut(s)	1285, 1534
BcnI	CCSGG	1 cut(s)	1691
BcoDI	GTCTC	4 cut(s)	1088, 1279, 1720, 2253
BcuI	ACTAGT	1 cut(s)	1951
BfaI	CTAG	7 cut(s)	969, 1014, 1121, 1952, 2204, 2270, 2524
BfuAI	ACCTGC	3 cut(s)	1057, 1423, 2390
BfuI	GTATCC	1 cut(s)	976
BglII	AGATCT	2 cut(s)	2051, 2303
BisI	GCNGC	2 cut(s)	1848, 2325
BlsI	GCNGC	2 cut(s)	1849, 2326
BmcAI	AGTACT	1 cut(s)	1972
Bme1390I	CCNGG	2 cut(s)	1691, 1882
Bme18I	GGWCC	1 cut(s)	394
BmeT110I	CYCGRG	1 cut(s)	2256
BmgBI	CACGTC	2 cut(s)	550, 2336
BmgT120I	GGNCC	1 cut(s)	394
BmiI	GGNNCC	4 cut(s)	90, 109, 1501, 2028
BmrFI	CCNGG	2 cut(s)	1691, 1882
BmsI	GCATC	8 cut(s)	193, 295, 713, 869, 1164, 1910, 2003, 2146
BmtI	GCTAGC	2 cut(s)	972, 2207
BpiI	GAAGAC	1 cut(s)	843
BplI	GAGNNNNNCTC	2 cut(s)	1790, 1822
BpmI	CTGGAG	1 cut(s)	876
Bpu10I	CCTNAGC	1 cut(s)	1518
Bpu14I	TTCGAA	1 cut(s)	2451
BpuEI	CTTGAG	4 cut(s)	1489, 1626, 1827, 1987
BpuMI	CCSGG	1 cut(s)	1691
BsaI	GGTCTC	1 cut(s)	1279
BsaJI	CCNNGG	4 cut(s)	885, 915, 1089, 1689
BsaWI	WCCGGW	3 cut(s)	367, 2084, 2108
BsaXI	ACNNNNNCTCC	6 cut(s)	42, 72, 866, 896, 2457, 2487
Bsc4I	CCNNNNNNNGG	6 cut(s)	425, 635, 1612, 1670, 1697, 2108
Bse118I	RCCGGY	2 cut(s)	367, 414
Bse1I	ACTGG	2 cut(s)	563, 1788
Bse3DI	GCAATG	1 cut(s)	2292
BseAI	TCCGGA	1 cut(s)	2108
BseBI	CCWGG	1 cut(s)	1882
BseDI	CCNNGG	4 cut(s)	885, 915, 1089, 1689
BseGI	GGATG	5 cut(s)	127, 202, 241, 310, 331
BseLI	CCNNNNNNNGG	6 cut(s)	425, 635, 1612, 1670, 1697, 2108
BseMI	GCAATG	1 cut(s)	2292
BseMII	CTCAG	6 cut(s)	153, 1269, 1330, 1532, 1865, 2018
BseNI	ACTGG	2 cut(s)	563, 1788
BseSI	GKGCMC	1 cut(s)	774
BseX3I	CGGCCG	1 cut(s)	416
BseXI	GCAGC	2 cut(s)	1834, 2311
Bsh1285I	CGRYCG	1 cut(s)	419
BshFI	GGCC	7 cut(s)	414, 418, 1383, 1443, 1787, 2074, 2433
BshTI	ACCGGT	1 cut(s)	367
BsiEI	CGRYCG	1 cut(s)	419
BsiHKAI	GWGCWC	2 cut(s)	1711, 2343
BsiHKCI	CYCGRG	1 cut(s)	2256
BsiSI	CCGG	5 cut(s)	368, 415, 1691, 2085, 2109
BslFI	GGGAC	2 cut(s)	2152, 2239
BslI	CCNNNNNNNGG	6 cut(s)	425, 635, 1612, 1670, 1697, 2108
BsmAI	GTCTC	4 cut(s)	1088, 1279, 1720, 2253
BsmBI	CGTCTC	2 cut(s)	1720, 2253
BsmFI	GGGAC	2 cut(s)	2152, 2239
BsmI	GAATGC	2 cut(s)	972, 1364
BsnI	GGCC	7 cut(s)	414, 418, 1383, 1443, 1787, 2074, 2433
Bso31I	GGTCTC	1 cut(s)	1279
BsoBI	CYCGRG	1 cut(s)	2256
Bsp119I	TTCGAA	1 cut(s)	2451
Bsp1286I	GDGCHC	3 cut(s)	774, 1711, 2343
Bsp13I	TCCGGA	1 cut(s)	2108
Bsp1407I	TGTACA	3 cut(s)	991, 1352, 1582
BspACI	CCGC	4 cut(s)	604, 2130, 2331, 2501
BspANI	GGCC	7 cut(s)	414, 418, 1383, 1443, 1787, 2074, 2433
BspCNI	CTCAG	6 cut(s)	154, 1270, 1329, 1531, 1864, 2017
BspEI	TCCGGA	1 cut(s)	2108
BspLI	GGNNCC	4 cut(s)	90, 109, 1501, 2028
BspMI	ACCTGC	3 cut(s)	1057, 1423, 2390
BspOI	GCTAGC	2 cut(s)	972, 2207
BspPI	GGATC	7 cut(s)	486, 1494, 1507, 2021, 2034, 2158, 2489
BspT104I	TTCGAA	1 cut(s)	2451
BspTNI	GGTCTC	1 cut(s)	1279
BsrDI	GCAATG	1 cut(s)	2292
BsrFI	RCCGGY	2 cut(s)	367, 414
BsrGI	TGTACA	3 cut(s)	991, 1352, 1582
BsrI	ACTGG	2 cut(s)	563, 1788
BssAI	RCCGGY	2 cut(s)	367, 414
BssECI	CCNNGG	4 cut(s)	885, 915, 1089, 1689
BssT1I	CCWWGG	1 cut(s)	915
Bst2UI	CCWGG	1 cut(s)	1882
Bst6I	CTCTTC	5 cut(s)	207, 243, 348, 885, 1099
BstAPI	GCANNNNNTGC	1 cut(s)	606
BstAUI	TGTACA	3 cut(s)	991, 1352, 1582
BstBI	TTCGAA	1 cut(s)	2451
BstDEI	CTNAG	6 cut(s)	162, 1278, 1316, 1518, 1851, 2004
BstENI	CCTNNNNNAGG	2 cut(s)	633, 1668
BstF5I	GGATG	5 cut(s)	127, 202, 241, 310, 331
BstHHI	GCGC	1 cut(s)	2387
BstMAI	GTCTC	4 cut(s)	1088, 1279, 1720, 2253
BstMCI	CGRYCG	1 cut(s)	419
BstMWI	GCNNNNNNNGC	2 cut(s)	606, 1194
BstNI	CCWGG	1 cut(s)	1882
BstSCI	CCNGG	2 cut(s)	1689, 1880
BstSLI	GKGCMC	1 cut(s)	774
BstV1I	GCAGC	2 cut(s)	1834, 2311
BstV2I	GAAGAC	1 cut(s)	843
BstX2I	RGATCY	6 cut(s)	1499, 2026, 2051, 2150, 2303, 2481
BstYI	RGATCY	6 cut(s)	1499, 2026, 2051, 2150, 2303, 2481
BstZI	CGGCCG	1 cut(s)	416
BsuI	GTATCC	1 cut(s)	976
BsuRI	GGCC	7 cut(s)	414, 418, 1383, 1443, 1787, 2074, 2433
BtrI	CACGTC	2 cut(s)	550, 2336
BtsCI	GGATG	5 cut(s)	127, 202, 241, 310, 331
BtsI	GCAGTG	1 cut(s)	2510
BtsIMutI	CAGTG	4 cut(s)	570, 966, 2313, 2510
BveI	ACCTGC	3 cut(s)	1057, 1423, 2390
CaiI	CAGNNNCTG	2 cut(s)	965, 2349
CfoI	GCGC	1 cut(s)	2387
Cfr10I	RCCGGY	2 cut(s)	367, 414
Cfr13I	GGNCC	1 cut(s)	394
CseI	GACGC	2 cut(s)	195, 1737
CspAI	ACCGGT	1 cut(s)	367
CviAII	CATG	4 cut(s)	28, 757, 1159, 1387
DdeI	CTNAG	6 cut(s)	162, 1278, 1316, 1518, 1851, 2004
EaeI	YGGCCR	4 cut(s)	412, 416, 1441, 1785
EagI	CGGCCG	1 cut(s)	416
Eam1104I	CTCTTC	5 cut(s)	207, 243, 348, 885, 1099
EarI	CTCTTC	5 cut(s)	207, 243, 348, 885, 1099
EclXI	CGGCCG	1 cut(s)	416
Eco130I	CCWWGG	1 cut(s)	915
Eco31I	GGTCTC	1 cut(s)	1279
Eco47I	GGWCC	1 cut(s)	394
Eco52I	CGGCCG	1 cut(s)	416
Eco57I	CTGAAG	2 cut(s)	1476, 2110
Eco88I	CYCGRG	1 cut(s)	2256
EcoNI	CCTNNNNNAGG	2 cut(s)	633, 1668
EcoRI	GAATTC	2 cut(s)	518, 2092
EcoRII	CCWGG	1 cut(s)	1880
EcoT14I	CCWWGG	1 cut(s)	915
ErhI	CCWWGG	1 cut(s)	915
Esp3I	CGTCTC	2 cut(s)	1720, 2253
FaeI	CATG	4 cut(s)	31, 760, 1162, 1390
FalI	AAGNNNNNCTT	4 cut(s)	593, 625, 2468, 2500
FaqI	GGGAC	2 cut(s)	2152, 2239
FatI	CATG	4 cut(s)	27, 756, 1158, 1386
FauI	CCCGC	1 cut(s)	2508
FbaI	TGATCA	2 cut(s)	1285, 1534
FblI	GTMKAC	2 cut(s)	1483, 2134
Fnu4HI	GCNGC	2 cut(s)	1848, 2325
FokI	GGATG	5 cut(s)	134, 209, 248, 317, 338
FseI	GGCCGGCC	1 cut(s)	418
Fsp4HI	GCNGC	2 cut(s)	1848, 2325
FspBI	CTAG	7 cut(s)	969, 1014, 1121, 1952, 2204, 2270, 2524
GlaI	GCGC	1 cut(s)	2386
GluI	GCNGC	2 cut(s)	1848, 2325
GsuI	CTGGAG	1 cut(s)	876
HaeIII	GGCC	7 cut(s)	414, 418, 1383, 1443, 1787, 2074, 2433
HapII	CCGG	5 cut(s)	368, 415, 1691, 2085, 2109
HgaI	GACGC	2 cut(s)	195, 1737
HhaI	GCGC	1 cut(s)	2387
Hin1II	CATG	4 cut(s)	31, 760, 1162, 1390
Hin6I	GCGC	1 cut(s)	2385
HinP1I	GCGC	1 cut(s)	2385
HinfI	GANTC	6 cut(s)	7, 116, 836, 1178, 1409, 2453
HpaII	CCGG	5 cut(s)	368, 415, 1691, 2085, 2109
HphI	GGTGA	7 cut(s)	155, 283, 580, 1276, 1404, 1566, 1614
Hpy166II	GTNNAC	5 cut(s)	1484, 1511, 1631, 2135, 2516
Hpy8I	GTNNAC	5 cut(s)	1484, 1511, 1631, 2135, 2516
Hpy99I	CGWCG	1 cut(s)	211
HpyCH4IV	ACGT	4 cut(s)	549, 2262, 2335, 2518
HpyCH4V	TGCA	6 cut(s)	1131, 1155, 1197, 1229, 2297, 2311
HpyF10VI	GCNNNNNNNGC	2 cut(s)	606, 1194
HpyF3I	CTNAG	6 cut(s)	162, 1278, 1316, 1518, 1851, 2004
HpySE526I	ACGT	4 cut(s)	549, 2262, 2335, 2518
Hsp92II	CATG	4 cut(s)	31, 760, 1162, 1390
HspAI	GCGC	1 cut(s)	2385
Kpn2I	TCCGGA	1 cut(s)	2108
KroI	GCCGGC	1 cut(s)	414
KroNI	GCCGGC	1 cut(s)	416
Ksp22I	TGATCA	2 cut(s)	1285, 1534
LmnI	GCTCC	3 cut(s)	857, 1706, 2063
Lsp1109I	GCAGC	2 cut(s)	1834, 2311
LweI	GCATC	8 cut(s)	193, 295, 713, 869, 1164, 1910, 2003, 2146
MaeI	CTAG	7 cut(s)	969, 1014, 1121, 1952, 2204, 2270, 2524
MaeII	ACGT	4 cut(s)	549, 2262, 2335, 2518
MaeIII	GTNAC	3 cut(s)	725, 781, 1389
MflI	RGATCY	6 cut(s)	1499, 2026, 2051, 2150, 2303, 2481
MhlI	GDGCHC	3 cut(s)	774, 1711, 2343
MlsI	TGGCCA	1 cut(s)	1787
MluNI	TGGCCA	1 cut(s)	1787
MmeI	TCCRAC	4 cut(s)	189, 1648, 1897, 2054
Mox20I	TGGCCA	1 cut(s)	1787
MroI	TCCGGA	1 cut(s)	2108
MroNI	GCCGGC	1 cut(s)	414
MroXI	GAANNNNTTC	1 cut(s)	2092
MscI	TGGCCA	1 cut(s)	1787
MseI	TTAA	7 cut(s)	432, 1209, 1608, 1733, 1761, 1982, 2399
Msp20I	TGGCCA	1 cut(s)	1787
MspA1I	CMGCKG	1 cut(s)	2130
MspI	CCGG	5 cut(s)	368, 415, 1691, 2085, 2109
MspR9I	CCNGG	2 cut(s)	1691, 1882
Mva1269I	GAATGC	2 cut(s)	972, 1364
MvaI	CCWGG	1 cut(s)	1882
MwoI	GCNNNNNNNGC	2 cut(s)	606, 1194
NaeI	GCCGGC	1 cut(s)	416
NciI	CCSGG	1 cut(s)	1691
NgoMIV	GCCGGC	1 cut(s)	414
NheI	GCTAGC	2 cut(s)	968, 2203
NlaIII	CATG	4 cut(s)	31, 760, 1162, 1390
NlaIV	GGNNCC	4 cut(s)	90, 109, 1501, 2028
NmeAIII	GCCGAG	1 cut(s)	1469
NmuCI	GTSAC	1 cut(s)	725
NspV	TTCGAA	1 cut(s)	2451
PaqCI	CACCTGC	1 cut(s)	1423
PctI	GAATGC	2 cut(s)	972, 1364
PdiI	GCCGGC	1 cut(s)	416
PdmI	GAANNNNTTC	1 cut(s)	2092
PfeI	GAWTC	6 cut(s)	7, 116, 836, 1178, 1409, 2453
PinAI	ACCGGT	1 cut(s)	367
PkrI	GCNGC	2 cut(s)	1849, 2326
PsiI	TTATAA	1 cut(s)	489
Psp6I	CCWGG	1 cut(s)	1880
PspGI	CCWGG	1 cut(s)	1880
PspN4I	GGNNCC	4 cut(s)	90, 109, 1501, 2028
PspPI	GGNCC	1 cut(s)	394
PstNI	CAGNNNCTG	2 cut(s)	965, 2349
PsuI	RGATCY	6 cut(s)	1499, 2026, 2051, 2150, 2303, 2481
RigI	GGCCGGCC	1 cut(s)	418
SaqAI	TTAA	7 cut(s)	432, 1209, 1608, 1733, 1761, 1982, 2399
SatI	GCNGC	2 cut(s)	1848, 2325
Sau96I	GGNCC	1 cut(s)	394
ScaI	AGTACT	1 cut(s)	1972
ScrFI	CCNGG	2 cut(s)	1691, 1882
SduI	GDGCHC	3 cut(s)	774, 1711, 2343
SfaNI	GCATC	8 cut(s)	193, 295, 713, 869, 1164, 1910, 2003, 2146
SfuI	TTCGAA	1 cut(s)	2451
SinI	GGWCC	1 cut(s)	394
SmlI	CTYRAG	4 cut(s)	1504, 1641, 1842, 1966
SmoI	CTYRAG	4 cut(s)	1504, 1641, 1842, 1966
SpeI	ACTAGT	1 cut(s)	1951
SsiI	CCGC	4 cut(s)	604, 2130, 2331, 2501
SspI	AATATT	1 cut(s)	2143
SspMI	CTAG	7 cut(s)	969, 1014, 1121, 1952, 2204, 2270, 2524
StyD4I	CCNGG	2 cut(s)	1689, 1880
StyI	CCWWGG	1 cut(s)	915
TaiI	ACGT	4 cut(s)	552, 2265, 2338, 2521
TaqI	TCGA	7 cut(s)	286, 707, 1562, 1657, 2022, 2451, 2487
TatI	WGTACW	5 cut(s)	991, 1352, 1582, 1970, 2077
TfiI	GAWTC	6 cut(s)	7, 116, 836, 1178, 1409, 2453
Tru1I	TTAA	7 cut(s)	432, 1209, 1608, 1733, 1761, 1982, 2399
Tru9I	TTAA	7 cut(s)	432, 1209, 1608, 1733, 1761, 1982, 2399
TscAI	CASTG	4 cut(s)	570, 966, 2320, 2510
TseFI	GTSAC	1 cut(s)	725
TseI	GCWGC	2 cut(s)	1847, 2324
Tsp45I	GTSAC	1 cut(s)	725
TspDTI	ATGAA	8 cut(s)	168, 366, 510, 546, 552, 745, 945, 2105
TspGWI	ACGGA	2 cut(s)	81, 2198
TspRI	CASTG	4 cut(s)	570, 966, 2320, 2510
VpaK11BI	GGWCC	1 cut(s)	394
XagI	CCTNNNNNAGG	2 cut(s)	633, 1668
XapI	RAATTY	4 cut(s)	518, 539, 2092, 2104
XbaI	TCTAGA	1 cut(s)	2269
XmiI	GTMKAC	2 cut(s)	1483, 2134
XmnI	GAANNNNTTC	1 cut(s)	2092
XspI	CTAG	7 cut(s)	969, 1014, 1121, 1952, 2204, 2270, 2524
ZrmI	AGTACT	1 cut(s)	1972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.