Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006287

Physical Location & Seq

Genomic Coordinates Reverse (-)

28828964 .. 28832661

Gene Structure 3698 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1209300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGCTCTCGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGGAGAAACAGAAGCATTTCGGACCATTTTCCATTGTTTCCGGTGCTCGCTTTCTCTCTCAGGTGTATTTTATGTATGGTAAAGGGAGGGAAGGTTAGTTCCAAGCGAAAAATCAAGACGAGGATTCCGTCAAAGGATCAGGGTTCCGATGATTCGGATGAGGATTATGTGATTTCGGGGGAAGAAAATGAAGTTTCTGAGGATTTAGAGGAGGATTACTGTTCTTCTTTGGATGGAGATGAGTCAGAAGAGAGCTTTGGGAGTTTTGTGGACGAGGAGGAAGAGGAGGAAGAGGAGGAGGAAGTGAAAAAGGTGAGGAAGAACAGTAGATCAAGAGCGAAAAGGGGTTCTGCTGGTTTTATTGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGACAAGGAGGAAGTGAAGCCGGTTAGGAAGGGAATCAGGTCAAAAGTCCGAAATGGTTTTTCTGGTCGGCAGAAAAATGGGGTTAAACCGTTACGAAAGAGGAGAATGATTGCATATCGTGAGGTGGAGGATCAGGATTATAATGATGAAGATGATGATGACGATGAGGAATTTACACCAGATGAAGATGAATTTTCAGAGGTGGAAGAAAAACATGTGGTGAAAAAGAAGAAACTGGGCAAGCGGCAGTTGAAGAAAAAGGGTCGTTCTGTAAGAGGTGAAAAAAGGAGAAAATCTATGGCTTTAAAGAAGCCTTTGAGAAAGAAAGGTAGAACGAGTCAGGAGTTGGGGAGGAAGGCAAGAAGGGATGATGAACTTGAAAGTGATAGTGATTTTATTGATAGTATTGTGCCCTTGAGAGTAACGAATGAGAAAAAAAGGGTTAGGAAAAGGAGGAGAATGTCCGTACCGTCAGATTCAGATTTTGTGCCTTCTGGAGCATCTGATTTTGACTACACCATCTCCGAGGAAGAGAGAGAGCAAGTACGAGAAGCCAAGGAGTTGTGTGAAAGATTGGAAACTAATTTGCAAAGTTCATCAGTGCCTACTAGCCTTCAGGAGGATACGCTTGTACAGCAGCCAAGAAAGCCTCCTAGAAGGAAGGGCAAAGAGAAGATTGAGCAAGTGAAGGTTGAGGTTGTAAAGCAGGTCTGTGGAATTTGTTTTTCCGAGGAAGATAAAAGAAGAGTGAGGGGAACATTAGACTGTTGCACTCATTATTTTTGTTTTGCATGCATCATGGAATGGGGAAAAGTGGAATCTCGTTGCCCTTTGTGCAAGCAAAGGTTTAAGACAATCAGTAAGCCTGCAAGATCTACGACAGGAATTGATTTGAGAGAAGTGGTGATACAGGTCCCTGAGCGTGATCAGGTCTATCAACCGACGGAAGAAGAACTCAGGGGTTATCTTGACCCTTACGAGAATGTGATCTGTACAGAATGCCACGAAGGAGGGGAAGATGGCCTCATGCTACTGTGTGATATATGCGATTCACCTGCTCACACCTTTTGTGTTGGTCTTGGGCGAGAAGTACCTGATGGTAATTGGTACTGTGAGGGTTGTAGACCTGTAGCTATGGGATCATCAAGTTCACAGGCTCAGGGTCGGTCGTCTGATCAAAGAAATACAAACAACATTCTGTCTAATAGACCATCACCTGCTGTATATTTTGACGAAGGGATAGACCTTAATGTGGTGTCCTCACCTTGTCCACAATTCACTCAAGGATTTTCCCCAAGAATTCCTGTTGGAGGTTTTCAAGCACCTTCCCCGTTGTCTGGGGCTGCAGCACCGACTCTATCAGGGAGACGCTTAATACAGCGTCATATTCAACAACTCCTTAATCTTAACAGGACGACTTATATGGCCAGTATGAATGAGAGGAACTCATCCAATAATTTGAGTAATGATTTCTCAAACTCTCAAGCTGCTCGGGCGAGGGAAATGACAGTCCAACATACCAGGACAGACGAGATGGAGGCAGCTTATCATACATTCTTTGAGGAGAGATTACAAGATAATCATTTAGCACTAGTGCCAGATAATTTGAGTACTGATTTCTTAAATTCATCAGCATCAACCCCTCAGAGAAGGCAAGCAATCGAGGATCCACCTACTACATCTGCTAATAGATCTCTAAATGGAGCATTATGGCCTGAACTTACCGGGATGAACTCAGTACCAAATTACGGACAATTCCATCACTTAAGCAGTAGACCAAACATTAGTGGATCTGATGCTGGTGTGTCCCCTAATACAGCTGTAGAACAGAGTGGCTTTCACCTAGCAAAGGAAAAACTGAAAGCGATGGTGAAAAACCGCTTGGATAATTTGTCCCGAGATGTTGATCTAGACAAAGATACTTTCAATGACATTGCAAGGAGATCGACACACACTGTCTTGGCTGCGTGCGGACACCAGCACAGAAGCAACGAGGTAGTTGTGGTTCCTCCCCCATCAACCTGCTCCCACATTGAATTAACAGCGTGTGGAATGACAAGTCTGATGAGAGGTTGCTGTTCATGTTGTTTCGAATCTTTTGTAGGGGAAGTCGTAAAGAGGATCCTCGATACCAAACTCCAACATTGGTTGAGTTTAGCTCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008623 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016590 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019899 GO:0019904 GO:0031010 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070063 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD zf-RING_2

Protein Analysis

867

Amino Acids

98.24

Weight (kDa)

5.67

Isoelectric Point (pI)

67.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	382 - 425	3.4e-06	Ring finger domain
PHD	PF00628	476 - 519	2e-07	PHD-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006287)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05670 AT3G05670 AT3G05670
fragaria_vesca	FvH4_6g07080
malus_domestica	MD04G1196300.v1.1 MD12G1209300.v1.1
prunus_persica	Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1
pyrus_communis	pycom04g17360 pycom12g19660
rosa_chinensis	RchiOBHm_Chr3g0456661
rosa_laevigata	RLG00000025286
rosa_multiflora	Rmu_co8126688.1_g000001 Rmu_sc0000133.1_g000031
rosa_roxburghii	Rroxscaffold_11G00450420 Rroxscaffold_6G00422560
rosa_rugosa	Rorug03G0014500 Rorug03G0014600 Rorug03G0014700 Rorug03G0014800.1
rosa_samantha	Rh3AG075300 Rh3BG077200 Rh3CG076900 Rh3DG078900
rosa_wichuraiana	Rw3G006450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	576
AarI	CACCTGC	2 cut(s)	1498, 1660
Acc36I	ACCTGC	4 cut(s)	1132, 1498, 1660, 2465
AccI	GTMKAC	2 cut(s)	1558, 2209
AciI	CCGC	3 cut(s)	679, 2314, 2406
AclWI	GGATC	8 cut(s)	180, 573, 1582, 2096, 2109, 2233, 2551, 2564
AcoI	YGGCCR	1 cut(s)	1860
AcsI	RAATTY	5 cut(s)	605, 626, 1152, 1734, 2059
AcuI	CTGAAG	1 cut(s)	1034
AfaI	GTAC	8 cut(s)	903, 981, 1068, 1429, 1527, 1544, 2047, 2175
AfiI	CCNNNNNNNGG	6 cut(s)	1054, 1687, 1745, 1772, 2183, 2284
AflII	CTTAAG	1 cut(s)	2200
AflIII	ACRYGT	1 cut(s)	649
AgsI	TTSAA	6 cut(s)	688, 815, 1754, 1826, 2362, 2471
AhlI	ACTAGT	1 cut(s)	2026
AjnI	CCWGG	1 cut(s)	1955
AjuI	GAANNNNNNNTTGG	4 cut(s)	276, 308, 2300, 2332
AloI	GAACNNNNNNTCC	2 cut(s)	118, 150
AluBI	AGCT	6 cut(s)	291, 1568, 1922, 1979, 2255, 2594
AluI	AGCT	6 cut(s)	291, 1568, 1922, 1979, 2255, 2594
Alw21I	GWGCWC	1 cut(s)	84
Alw26I	GTCTC	1 cut(s)	1795
AlwI	GGATC	8 cut(s)	180, 573, 1582, 2096, 2109, 2233, 2551, 2564
Ama87I	CYCGRG	3 cut(s)	7, 1926, 2331
AoxI	GGCC	3 cut(s)	1456, 1860, 2147
ApeKI	GCWGC	6 cut(s)	1072, 1778, 1781, 1922, 1976, 2399
ApoI	RAATTY	5 cut(s)	605, 626, 1152, 1734, 2059
Asp700I	GAANNNNTTC	1 cut(s)	52
AspS9I	GGNCC	2 cut(s)	58, 1348
AsuC2I	CCSGG	1 cut(s)	2161
AsuHPI	GGTGA	9 cut(s)	361, 667, 725, 1351, 1479, 1641, 1689, 2267, 2317
AsuII	TTCGAA	1 cut(s)	2526
AvaI	CYCGRG	3 cut(s)	7, 1926, 2331
AvaII	GGWCC	2 cut(s)	58, 1348
BaeGI	GKGCMC	1 cut(s)	849
BaeI	ACNNNNGTAYC	2 cut(s)	1050, 1083
BalI	TGGCCA	1 cut(s)	1862
BamHI	GGATCC	2 cut(s)	2101, 2556
BarI	GAAGNNNNNNTAC	2 cut(s)	1326, 1358
BbsI	GAAGAC	1 cut(s)	441
Bbv12I	GWGCWC	1 cut(s)	84
BbvI	GCAGC	6 cut(s)	1084, 1765, 1793, 1909, 1988, 2386
BccI	CCATC	9 cut(s)	263, 962, 1448, 1526, 1654, 1963, 2202, 2296, 2458
BcgI	CGANNNNNNTGC	4 cut(s)	1472, 1506, 2361, 2395
BciT130I	CCWGG	1 cut(s)	1957
BciVI	GTATCC	1 cut(s)	1051
BclI	TGATCA	2 cut(s)	1360, 1609
BcnI	CCSGG	1 cut(s)	2161
BcoDI	GTCTC	1 cut(s)	1795
BcuI	ACTAGT	1 cut(s)	2026
BfaI	CTAG	6 cut(s)	1044, 1089, 2027, 2279, 2345, 2599
BfmI	CTRYAG	3 cut(s)	1563, 1779, 2256
BfrI	CTTAAG	1 cut(s)	2200
BfuAI	ACCTGC	4 cut(s)	1132, 1498, 1660, 2465
BfuI	GTATCC	1 cut(s)	1051
BglII	AGATCT	2 cut(s)	1307, 2126
BisI	GCNGC	7 cut(s)	680, 1073, 1779, 1782, 1923, 1977, 2400
BlsI	GCNGC	7 cut(s)	681, 1074, 1780, 1783, 1924, 1978, 2401
BmcAI	AGTACT	1 cut(s)	2047
Bme1390I	CCNGG	2 cut(s)	1957, 2161
Bme18I	GGWCC	2 cut(s)	58, 1348
BmeT110I	CYCGRG	3 cut(s)	7, 1926, 2331
BmgT120I	GGNCC	2 cut(s)	58, 1348
BmiI	GGNNCC	5 cut(s)	181, 1350, 2103, 2442, 2558
BmrFI	CCNGG	2 cut(s)	1957, 2161
BmrI	ACTGGG	1 cut(s)	680
BmsI	GCATC	4 cut(s)	944, 1239, 2078, 2221
BmuI	ACTGGG	1 cut(s)	680
BpiI	GAAGAC	1 cut(s)	441
BplI	GAGNNNNNCTC	3 cut(s)	1865, 1897, 2578
BpmI	CTGGAG	1 cut(s)	951
Bpu10I	CCTNAGC	2 cut(s)	1353, 1593
Bpu14I	TTCGAA	1 cut(s)	2526
BpuEI	CTTGAG	3 cut(s)	871, 1701, 1902
BpuMI	CCSGG	1 cut(s)	2161
BsaBI	GATNNNNATC	1 cut(s)	2340
BsaJI	CCNNGG	3 cut(s)	960, 990, 1164
BsaWI	WCCGGW	1 cut(s)	76
BsaXI	ACNNNNNCTCC	4 cut(s)	114, 144, 941, 971
Bsc4I	CCNNNNNNNGG	6 cut(s)	1054, 1687, 1745, 1772, 2183, 2284
Bse118I	RCCGGY	1 cut(s)	454
Bse1I	ACTGG	2 cut(s)	675, 1863
Bse3DI	GCAATG	1 cut(s)	2367
Bse8I	GATNNNNATC	1 cut(s)	2340
BseBI	CCWGG	1 cut(s)	1957
BseDI	CCNNGG	3 cut(s)	960, 990, 1164
BseGI	GGATG	5 cut(s)	199, 274, 808, 1883, 2169
BseJI	GATNNNNATC	1 cut(s)	2340
BseLI	CCNNNNNNNGG	6 cut(s)	1054, 1687, 1745, 1772, 2183, 2284
BseMI	GCAATG	1 cut(s)	2367
BseMII	CTCAG	7 cut(s)	109, 225, 1344, 1405, 1607, 2093, 2184
BseNI	ACTGG	2 cut(s)	675, 1863
BseSI	GKGCMC	1 cut(s)	849
BseXI	GCAGC	6 cut(s)	1084, 1765, 1793, 1909, 1988, 2386
Bsh1285I	CGRYCG	1 cut(s)	1604
BshFI	GGCC	3 cut(s)	1458, 1862, 2149
BsiEI	CGRYCG	1 cut(s)	1604
BsiHKAI	GWGCWC	1 cut(s)	84
BsiHKCI	CYCGRG	3 cut(s)	7, 1926, 2331
BsiSI	CCGG	3 cut(s)	77, 455, 2160
BslFI	GGGAC	3 cut(s)	1334, 2227, 2314
BslI	CCNNNNNNNGG	6 cut(s)	1054, 1687, 1745, 1772, 2183, 2284
BsmAI	GTCTC	1 cut(s)	1795
BsmBI	CGTCTC	1 cut(s)	1795
BsmFI	GGGAC	3 cut(s)	1334, 2227, 2314
BsmI	GAATGC	1 cut(s)	1439
BsnI	GGCC	3 cut(s)	1458, 1862, 2149
BsoBI	CYCGRG	3 cut(s)	7, 1926, 2331
Bsp119I	TTCGAA	1 cut(s)	2526
Bsp1286I	GDGCHC	2 cut(s)	84, 849
Bsp1407I	TGTACA	2 cut(s)	1066, 1427
BspACI	CCGC	3 cut(s)	679, 2314, 2406
BspANI	GGCC	3 cut(s)	1458, 1862, 2149
BspCNI	CTCAG	7 cut(s)	108, 226, 1345, 1404, 1606, 2092, 2183
BspLI	GGNNCC	5 cut(s)	181, 1350, 2103, 2442, 2558
BspMAI	CTGCAG	1 cut(s)	1783
BspMI	ACCTGC	4 cut(s)	1132, 1498, 1660, 2465
BspPI	GGATC	8 cut(s)	180, 573, 1582, 2096, 2109, 2233, 2551, 2564
BspT104I	TTCGAA	1 cut(s)	2526
BspTI	CTTAAG	1 cut(s)	2200
BsrDI	GCAATG	1 cut(s)	2367
BsrFI	RCCGGY	1 cut(s)	454
BsrGI	TGTACA	2 cut(s)	1066, 1427
BsrI	ACTGG	2 cut(s)	675, 1863
BssAI	RCCGGY	1 cut(s)	454
BssECI	CCNNGG	3 cut(s)	960, 990, 1164
BssT1I	CCWWGG	1 cut(s)	990
Bst2UI	CCWGG	1 cut(s)	1957
Bst4CI	ACNGT	9 cut(s)	257, 362, 525, 906, 1202, 1470, 1547, 1945, 2392
Bst6I	CTCTTC	5 cut(s)	279, 312, 321, 960, 1174
BstAFI	CTTAAG	1 cut(s)	2200
BstAUI	TGTACA	2 cut(s)	1066, 1427
BstBI	TTCGAA	1 cut(s)	2526
BstC8I	GCNNGC	7 cut(s)	84, 677, 1228, 1274, 1302, 2091, 2404
BstDEI	CTNAG	7 cut(s)	95, 234, 1353, 1391, 1593, 2079, 2170
BstENI	CCTNNNNNAGG	3 cut(s)	1052, 1743, 2282
BstF5I	GGATG	5 cut(s)	199, 274, 808, 1883, 2169
BstMAI	GTCTC	1 cut(s)	1795
BstMCI	CGRYCG	1 cut(s)	1604
BstMWI	GCNNNNNNNGC	4 cut(s)	1069, 1081, 1269, 1928
BstNI	CCWGG	1 cut(s)	1957
BstNSI	RCATGY	2 cut(s)	653, 1230
BstSCI	CCNGG	2 cut(s)	1955, 2159
BstSFI	CTRYAG	3 cut(s)	1563, 1779, 2256
BstSLI	GKGCMC	1 cut(s)	849
BstV1I	GCAGC	6 cut(s)	1084, 1765, 1793, 1909, 1988, 2386
BstV2I	GAAGAC	1 cut(s)	441
BstX2I	RGATCY	5 cut(s)	1307, 2101, 2126, 2225, 2556
BstYI	RGATCY	5 cut(s)	1307, 2101, 2126, 2225, 2556
BsuI	GTATCC	1 cut(s)	1051
BsuRI	GGCC	3 cut(s)	1458, 1862, 2149
BtgZI	GCGATG	1 cut(s)	2315
BtsCI	GGATG	5 cut(s)	199, 274, 808, 1883, 2169
BtsIMutI	CAGTG	2 cut(s)	1041, 2388
BveI	ACCTGC	4 cut(s)	1132, 1498, 1660, 2465
Cac8I	GCNNGC	7 cut(s)	84, 677, 1228, 1274, 1302, 2091, 2404
Cfr10I	RCCGGY	1 cut(s)	454
Cfr13I	GGNCC	2 cut(s)	58, 1348
CseI	GACGC	2 cut(s)	1805, 1812
Csp6I	GTAC	8 cut(s)	902, 980, 1067, 1428, 1526, 1543, 2046, 2174
CviAII	CATG	5 cut(s)	650, 1227, 1234, 1462, 2517
CviQI	GTAC	8 cut(s)	902, 980, 1067, 1428, 1526, 1543, 2046, 2174
DdeI	CTNAG	7 cut(s)	95, 234, 1353, 1391, 1593, 2079, 2170
DraI	TTTAAA	1 cut(s)	741
EaeI	YGGCCR	1 cut(s)	1860
Eam1104I	CTCTTC	5 cut(s)	279, 312, 321, 960, 1174
EarI	CTCTTC	5 cut(s)	279, 312, 321, 960, 1174
Eco130I	CCWWGG	1 cut(s)	990
Eco47I	GGWCC	2 cut(s)	58, 1348
Eco57I	CTGAAG	1 cut(s)	1034
Eco88I	CYCGRG	3 cut(s)	7, 1926, 2331
EcoNI	CCTNNNNNAGG	3 cut(s)	1052, 1743, 2282
EcoO109I	RGGNCCY	1 cut(s)	1348
EcoRI	GAATTC	1 cut(s)	1734
EcoRII	CCWGG	1 cut(s)	1955
EcoT14I	CCWWGG	1 cut(s)	990
EcoT22I	ATGCAT	1 cut(s)	1232
ErhI	CCWWGG	1 cut(s)	990
Esp3I	CGTCTC	1 cut(s)	1795
FaeI	CATG	5 cut(s)	653, 1230, 1237, 1465, 2520
FaqI	GGGAC	3 cut(s)	1334, 2227, 2314
FatI	CATG	5 cut(s)	649, 1226, 1233, 1461, 2516
FbaI	TGATCA	2 cut(s)	1360, 1609
FblI	GTMKAC	2 cut(s)	1558, 2209
Fnu4HI	GCNGC	7 cut(s)	680, 1073, 1779, 1782, 1923, 1977, 2400
FokI	GGATG	5 cut(s)	206, 281, 815, 1870, 2176
Fsp4HI	GCNGC	7 cut(s)	680, 1073, 1779, 1782, 1923, 1977, 2400
FspBI	CTAG	6 cut(s)	1044, 1089, 2027, 2279, 2345, 2599
GluI	GCNGC	7 cut(s)	680, 1073, 1779, 1782, 1923, 1977, 2400
GsuI	CTGGAG	1 cut(s)	951
HaeIII	GGCC	3 cut(s)	1458, 1862, 2149
HapII	CCGG	3 cut(s)	77, 455, 2160
HgaI	GACGC	2 cut(s)	1805, 1812
Hin1II	CATG	5 cut(s)	653, 1230, 1237, 1465, 2520
HpaII	CCGG	3 cut(s)	77, 455, 2160
HphI	GGTGA	9 cut(s)	361, 667, 725, 1351, 1479, 1641, 1689, 2267, 2317
Hpy166II	GTNNAC	5 cut(s)	307, 1559, 1586, 1706, 2210
Hpy8I	GTNNAC	5 cut(s)	307, 1559, 1586, 1706, 2210
Hpy99I	CGWCG	1 cut(s)	1381
HpyCH4III	ACNGT	9 cut(s)	257, 362, 525, 906, 1202, 1470, 1547, 1945, 2392
HpyCH4V	TGCA	9 cut(s)	548, 1024, 1206, 1226, 1230, 1272, 1304, 1781, 2372
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1069, 1081, 1269, 1928
HpyF3I	CTNAG	7 cut(s)	95, 234, 1353, 1391, 1593, 2079, 2170
Hsp92II	CATG	5 cut(s)	653, 1230, 1237, 1465, 2520
Ksp22I	TGATCA	2 cut(s)	1360, 1609
LmnI	GCTCC	3 cut(s)	932, 2138, 2465
Lsp1109I	GCAGC	6 cut(s)	1084, 1765, 1793, 1909, 1988, 2386
LweI	GCATC	4 cut(s)	944, 1239, 2078, 2221
MaeI	CTAG	6 cut(s)	1044, 1089, 2027, 2279, 2345, 2599
MaeIII	GTNAC	2 cut(s)	525, 856
MflI	RGATCY	5 cut(s)	1307, 2101, 2126, 2225, 2556
MhlI	GDGCHC	2 cut(s)	84, 849
MlsI	TGGCCA	1 cut(s)	1862
MluNI	TGGCCA	1 cut(s)	1862
MlyI	GAGTC	3 cut(s)	287, 781, 1783
MmeI	TCCRAC	3 cut(s)	1723, 1972, 2599
Mox20I	TGGCCA	1 cut(s)	1862
Mph1103I	ATGCAT	1 cut(s)	1232
MroXI	GAANNNNTTC	1 cut(s)	52
MscI	TGGCCA	1 cut(s)	1862
Msp20I	TGGCCA	1 cut(s)	1862
MspA1I	CMGCKG	1 cut(s)	2255
MspCI	CTTAAG	1 cut(s)	2200
MspI	CCGG	3 cut(s)	77, 455, 2160
MspR9I	CCNGG	2 cut(s)	1957, 2161
Mva1269I	GAATGC	1 cut(s)	1439
MvaI	CCWGG	1 cut(s)	1957
MwoI	GCNNNNNNNGC	4 cut(s)	1069, 1081, 1269, 1928
NciI	CCSGG	1 cut(s)	2161
NlaIII	CATG	5 cut(s)	653, 1230, 1237, 1465, 2520
NlaIV	GGNNCC	5 cut(s)	181, 1350, 2103, 2442, 2558
NsiI	ATGCAT	1 cut(s)	1232
NspI	RCATGY	2 cut(s)	653, 1230
NspV	TTCGAA	1 cut(s)	2526
PaeI	GCATGC	1 cut(s)	1230
PaeR7I	CTCGAG	1 cut(s)	7
PaqCI	CACCTGC	2 cut(s)	1498, 1660
PciI	ACATGT	1 cut(s)	649
PcsI	WCGNNNNNNNCGW	1 cut(s)	161
PctI	GAATGC	1 cut(s)	1439
PdmI	GAANNNNTTC	1 cut(s)	52
PfeI	GAWTC	7 cut(s)	160, 188, 468, 911, 1253, 1484, 2528
PkrI	GCNGC	7 cut(s)	681, 1074, 1780, 1783, 1924, 1978, 2401
PleI	GAGTC	3 cut(s)	286, 780, 1783
PpsI	GAGTC	3 cut(s)	286, 780, 1783
PpuMI	RGGWCCY	1 cut(s)	1348
PscI	ACATGT	1 cut(s)	649
PsiI	TTATAA	1 cut(s)	576
Psp5II	RGGWCCY	1 cut(s)	1348
Psp6I	CCWGG	1 cut(s)	1955
PspGI	CCWGG	1 cut(s)	1955
PspN4I	GGNNCC	5 cut(s)	181, 1350, 2103, 2442, 2558
PspPI	GGNCC	2 cut(s)	58, 1348
PspPPI	RGGWCCY	1 cut(s)	1348
PsrI	GAACNNNNNNTAC	2 cut(s)	2424, 2456
PstI	CTGCAG	1 cut(s)	1783
PsuI	RGATCY	5 cut(s)	1307, 2101, 2126, 2225, 2556
PvuII	CAGCTG	1 cut(s)	2255
RsaI	GTAC	8 cut(s)	903, 981, 1068, 1429, 1527, 1544, 2047, 2175
RsaNI	GTAC	8 cut(s)	902, 980, 1067, 1428, 1526, 1543, 2046, 2174
SatI	GCNGC	7 cut(s)	680, 1073, 1779, 1782, 1923, 1977, 2400
Sau96I	GGNCC	2 cut(s)	58, 1348
ScaI	AGTACT	1 cut(s)	2047
SchI	GAGTC	3 cut(s)	287, 781, 1783
ScrFI	CCNGG	2 cut(s)	1957, 2161
SduI	GDGCHC	2 cut(s)	84, 849
SfaNI	GCATC	4 cut(s)	944, 1239, 2078, 2221
SfcI	CTRYAG	3 cut(s)	1563, 1779, 2256
Sfr274I	CTCGAG	1 cut(s)	7
SfuI	TTCGAA	1 cut(s)	2526
SinI	GGWCC	2 cut(s)	58, 1348
SlaI	CTCGAG	1 cut(s)	7
SmlI	CTYRAG	5 cut(s)	7, 850, 1716, 1917, 2200
SmoI	CTYRAG	5 cut(s)	7, 850, 1716, 1917, 2200
SpeI	ACTAGT	1 cut(s)	2026
SphI	GCATGC	1 cut(s)	1230
SsiI	CCGC	3 cut(s)	679, 2314, 2406
SspMI	CTAG	6 cut(s)	1044, 1089, 2027, 2279, 2345, 2599
StyD4I	CCNGG	2 cut(s)	1955, 2159
StyI	CCWWGG	1 cut(s)	990
TaaI	ACNGT	9 cut(s)	257, 362, 525, 906, 1202, 1470, 1547, 1945, 2392
TaqI	TCGA	5 cut(s)	8, 2097, 2381, 2526, 2562
TaqII	GACCGA	1 cut(s)	1590
TatI	WGTACW	3 cut(s)	1066, 1427, 2045
TauI	GCSGC	1 cut(s)	682
TfiI	GAWTC	7 cut(s)	160, 188, 468, 911, 1253, 1484, 2528
TscAI	CASTG	2 cut(s)	1041, 2395
TseI	GCWGC	6 cut(s)	1072, 1778, 1781, 1922, 1976, 2399
TspGWI	ACGGA	4 cut(s)	153, 889, 1394, 2199
TspRI	CASTG	2 cut(s)	1041, 2395
Vha464I	CTTAAG	1 cut(s)	2200
VpaK11BI	GGWCC	2 cut(s)	58, 1348
XagI	CCTNNNNNAGG	3 cut(s)	1052, 1743, 2282
XapI	RAATTY	5 cut(s)	605, 626, 1152, 1734, 2059
XbaI	TCTAGA	1 cut(s)	2344
XceI	RCATGY	2 cut(s)	653, 1230
XhoI	CTCGAG	1 cut(s)	7
XmiI	GTMKAC	2 cut(s)	1558, 2209
XmnI	GAANNNNTTC	1 cut(s)	52
XspI	CTAG	6 cut(s)	1044, 1089, 2027, 2279, 2345, 2599
ZrmI	AGTACT	1 cut(s)	2047
Zsp2I	ATGCAT	1 cut(s)	1232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.