MD04G1198800.v1.1

Dormancy/auxin associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006178
Physical Location & Seq
Reverse (-)
28620832 .. 28623211
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1198800.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGGCCTACTTGATCAGCTTTGGGACGACACCCTCGCCGGGCCTCAGCCCGACAGCGGTCTTGGGAAACTTCGCAAGCACAAAACCTTCAGTTTCCGGTCCAGCTCCGCCACTGGATCGTCAGATGGCGGAAGCGTAAGATCGTTGGGTGGGAATTCGCCGGAAGAGGCGAAAATTACGCGGAGTATTATGATAGTGAAGCCCCCAGGTTATGGGAGCGCTAACGGCGGATCAGCTCCGGTTTCGCCAGCTGGGGTTTCTTCTCCAATTTCGCCAGCTGGGTCTACGCCGCCGGTGTCCCCGTTTTCTGGTGGCAGGGAGTCATTCGGGCGGTTTCGAAGGAGGTCAGCGTCGGATGCATACGAGAAGGCGAGCGAGGTCGTTGGAGGAAGGAGCGCCCGTTCTCCTTGCGACGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

140

Amino Acids

14.18

Weight (kDa)

9.79

Isoelectric Point (pI)

84.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 85 2.2e-08 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 212
AccI GTMKAC 1 cut(s) 284
AccII CGCG 1 cut(s) 181
AciI CCGC 7 cut(s) 57, 108, 129, 181, 228, 290, 331
AclWI GGATC 2 cut(s) 124, 238
AcsI RAATTY 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 73
AfeI AGCGCT 1 cut(s) 220
AfiI CCNNNNNNNGG 4 cut(s) 39, 56, 212, 308
AflIII ACRYGT 1 cut(s) 414
AjiI CACGTC 1 cut(s) 415
AjnI CCWGG 1 cut(s) 205
AloI GAACNNNNNNTCC 2 cut(s) 385, 417
AluBI AGCT 5 cut(s) 19, 105, 236, 251, 278
AluI AGCT 5 cut(s) 19, 105, 236, 251, 278
AlwI GGATC 2 cut(s) 124, 238
Aor51HI AGCGCT 1 cut(s) 220
AoxI GGCC 2 cut(s) 4, 41
ApoI RAATTY 1 cut(s) 154
AspLEI GCGC 2 cut(s) 221, 398
AspS9I GGNCC 3 cut(s) 4, 41, 99
AsuC2I CCSGG 1 cut(s) 40
AsuII TTCGAA 1 cut(s) 337
AvaII GGWCC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 119
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 207
BclI TGATCA 1 cut(s) 13
BcnI CCSGG 1 cut(s) 40
BfoI RGCGCY 2 cut(s) 222, 399
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
Bme1390I CCNGG 2 cut(s) 40, 207
Bme18I GGWCC 1 cut(s) 99
BmgBI CACGTC 1 cut(s) 415
BmgT120I GGNCC 3 cut(s) 4, 41, 99
BmrFI CCNGG 2 cut(s) 40, 207
BmsI GCATC 1 cut(s) 346
BoxI GACNNNNGTC 1 cut(s) 57
Bpu10I CCTNAGC 1 cut(s) 45
Bpu14I TTCGAA 1 cut(s) 337
BpuMI CCSGG 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 2 cut(s) 96, 238
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 56, 212, 308
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 4 cut(s) 39, 56, 212, 308
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 118
BseYI CCCAGC 2 cut(s) 251, 278
Bsh1236I CGCG 1 cut(s) 181
BshFI GGCC 2 cut(s) 6, 43
BsiSI CCGG 5 cut(s) 39, 97, 161, 239, 293
BslFI GGGAC 2 cut(s) 38, 283
BslI CCNNNNNNNGG 4 cut(s) 39, 56, 212, 308
BsmFI GGGAC 2 cut(s) 38, 283
BsnI GGCC 2 cut(s) 6, 43
Bsp119I TTCGAA 1 cut(s) 337
Bsp143I GATC 4 cut(s) 13, 116, 140, 230
BspACI CCGC 7 cut(s) 57, 108, 129, 181, 228, 290, 331
BspANI GGCC 2 cut(s) 6, 43
BspCNI CTCAG 1 cut(s) 58
BspFNI CGCG 1 cut(s) 181
BspPI GGATC 2 cut(s) 124, 238
BspT104I TTCGAA 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 4 cut(s) 13, 116, 140, 230
Bst2UI CCWGG 1 cut(s) 207
Bst6I CTCTTC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 337
BstC8I GCNNGC 4 cut(s) 77, 249, 276, 373
BstDEI CTNAG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 361
BstFNI CGCG 1 cut(s) 181
BstH2I RGCGCY 2 cut(s) 222, 399
BstHHI GCGC 2 cut(s) 221, 398
BstKTI GATC 4 cut(s) 16, 119, 143, 233
BstMBI GATC 4 cut(s) 13, 116, 140, 230
BstMWI GCNNNNNNNGC 2 cut(s) 225, 356
BstNI CCWGG 1 cut(s) 207
BstPAI GACNNNNGTC 1 cut(s) 57
BstSCI CCNGG 2 cut(s) 38, 205
BstUI CGCG 1 cut(s) 181
BsuRI GGCC 2 cut(s) 6, 43
BtrI CACGTC 1 cut(s) 415
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 4 cut(s) 77, 249, 276, 373
CfoI GCGC 2 cut(s) 221, 398
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 3 cut(s) 4, 41, 99
CseI GACGC 1 cut(s) 339
CviJI RGCY 9 cut(s) 6, 19, 43, 49, 105, 202, 236, 251, 278
CviKI_1 RGCY 9 cut(s) 6, 19, 43, 49, 105, 202, 236, 251, 278
DdeI CTNAG 1 cut(s) 45
DpnI GATC 4 cut(s) 15, 118, 142, 232
DpnII GATC 4 cut(s) 13, 116, 140, 230
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 3 cut(s) 97, 144, 243
Eco47I GGWCC 1 cut(s) 99
Eco47III AGCGCT 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 361
FaiI YATR 3 cut(s) 191, 213, 361
FaqI GGGAC 2 cut(s) 38, 283
FbaI TGATCA 1 cut(s) 13
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 290
GlaI GCGC 2 cut(s) 220, 397
GluI GCNGC 1 cut(s) 290
GsaI CCCAGC 2 cut(s) 255, 282
HaeII RGCGCY 2 cut(s) 222, 399
HaeIII GGCC 2 cut(s) 6, 43
HapII CCGG 5 cut(s) 39, 97, 161, 239, 293
HgaI GACGC 1 cut(s) 339
HhaI GCGC 2 cut(s) 221, 398
Hin6I GCGC 2 cut(s) 219, 396
HinP1I GCGC 2 cut(s) 219, 396
HinfI GANTC 1 cut(s) 320
HpaII CCGG 5 cut(s) 39, 97, 161, 239, 293
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 2 cut(s) 124, 355
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 2 cut(s) 355, 416
HpyAV CCTTC 4 cut(s) 97, 333, 361, 384
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 356
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 414
HspAI GCGC 2 cut(s) 219, 396
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 4 cut(s) 13, 116, 140, 230
LmnI GCTCC 4 cut(s) 110, 216, 241, 393
LweI GCATC 1 cut(s) 346
MaeII ACGT 1 cut(s) 414
MalI GATC 4 cut(s) 15, 118, 142, 232
MboI GATC 4 cut(s) 13, 116, 140, 230
MboII GAAGA 2 cut(s) 176, 252
MluCI AATT 3 cut(s) 154, 174, 267
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 2 cut(s) 333, 364
MnlI CCTC 6 cut(s) 44, 54, 160, 336, 370, 380
Mph1103I ATGCAT 1 cut(s) 361
MspA1I CMGCKG 3 cut(s) 57, 251, 278
MspI CCGG 5 cut(s) 39, 97, 161, 239, 293
MspR9I CCNGG 2 cut(s) 40, 207
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 225, 356
NciI CCSGG 1 cut(s) 40
NdeII GATC 4 cut(s) 13, 116, 140, 230
NsiI ATGCAT 1 cut(s) 361
NspV TTCGAA 1 cut(s) 337
PflMI CCANNNNNTGG 1 cut(s) 212
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PshAI GACNNNNGTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 2 cut(s) 251, 278
PspGI CCWGG 1 cut(s) 205
PspPI GGNCC 3 cut(s) 4, 41, 99
PvuII CAGCTG 2 cut(s) 251, 278
SatI GCNGC 1 cut(s) 290
Sau3AI GATC 4 cut(s) 13, 116, 140, 230
Sau96I GGNCC 3 cut(s) 4, 41, 99
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 40, 207
SfaNI GCATC 1 cut(s) 346
SfuI TTCGAA 1 cut(s) 337
SgrAI CRCCGGYG 1 cut(s) 292
SinI GGWCC 1 cut(s) 99
Sse9I AATT 3 cut(s) 154, 174, 267
SsiI CCGC 7 cut(s) 57, 108, 129, 181, 228, 290, 331
StyD4I CCNGG 2 cut(s) 38, 205
TaiI ACGT 1 cut(s) 417
TaqI TCGA 1 cut(s) 337
TasI AATT 3 cut(s) 154, 174, 267
TauI GCSGC 1 cut(s) 292
TscAI CASTG 1 cut(s) 118
TspRI CASTG 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 99
XapI RAATTY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.