MD12G1212000.v1.1

Dormancy/auxin associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006178
Physical Location & Seq
Reverse (-)
29092353 .. 29094440
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1212000.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGGACTTCGCCTACTTGACCAGCTTTGGGACGACACCGTCGCCGGGCCGCAGCCCGACAGCGGTCTCGGGAAACTGCGGAAGCACAAAACCTTCAGTTTCCGGTCCAGCTCCGCAACTGGATCGTCAGATGGTGGGAACGTACGATTGTACGGTGAGAATTCCCCGGAGGAGGTGAAAGTTACGAGGAGTATCATGATAATTAAGCCCCCAGGTTATGGGAGCGGTAACGGCGGATCAGCTCCGGTTTCGCCAGCTGGGTTTACTCCGCCAGCTGGGTTCACGCCTCCGGCTTCGCCAGCCGGATCTACGCCGCCGGTGTCCCCGTTTTCTGGTGGCAGGGAGCCATTCGGGCGGTTTCGAAGAAGGTCAGCATCGGATGCATACGAGAAGGCGAGCGAGGTCCCTGGAGCTAGGAGCGCCCGTTCTCCTTACGACGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

148

Amino Acids

15.31

Weight (kDa)

9.81

Isoelectric Point (pI)

79.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 9 - 86 2e-08 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccB7I CCANNNNNTGG 1 cut(s) 218
AccBSI CCGCTC 1 cut(s) 225
AciI CCGC 9 cut(s) 50, 63, 79, 114, 225, 234, 269, 314, 355
AclWI GGATC 3 cut(s) 130, 244, 313
AcsI RAATTY 1 cut(s) 160
AcuI CTGAAG 1 cut(s) 79
AfaI GTAC 2 cut(s) 144, 152
AfiI CCNNNNNNNGG 7 cut(s) 28, 45, 62, 172, 218, 275, 332
AflIII ACRYGT 1 cut(s) 438
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 2 cut(s) 211, 406
AloI GAACNNNNNNTCC 2 cut(s) 409, 441
AluBI AGCT 6 cut(s) 25, 111, 242, 257, 275, 413
AluI AGCT 6 cut(s) 25, 111, 242, 257, 275, 413
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 3 cut(s) 130, 244, 313
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 160
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 3 cut(s) 47, 105, 403
AsuC2I CCSGG 2 cut(s) 46, 167
AsuHPI GGTGA 2 cut(s) 167, 187
AsuII TTCGAA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 2 cut(s) 105, 403
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BciT130I CCWGG 2 cut(s) 213, 408
BcnI CCSGG 2 cut(s) 46, 167
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 352
BisI GCNGC 3 cut(s) 50, 53, 314
BlsI GCNGC 3 cut(s) 51, 54, 315
Bme1390I CCNGG 4 cut(s) 46, 167, 213, 408
Bme18I GGWCC 2 cut(s) 105, 403
BmeT110I CYCGRG 1 cut(s) 68
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 47, 105, 403
BmiI GGNNCC 2 cut(s) 345, 405
BmrFI CCNGG 4 cut(s) 46, 167, 213, 408
BmsI GCATC 2 cut(s) 370, 383
BoxI GACNNNNGTC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 361
BpuMI CCSGG 2 cut(s) 46, 167
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 165, 211, 406
BsaWI WCCGGW 2 cut(s) 102, 244
BsaXI ACNNNNNCTCC 2 cut(s) 409, 439
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 45, 62, 172, 218, 275, 332
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 124
BseBI CCWGG 2 cut(s) 213, 408
BseDI CCNNGG 3 cut(s) 165, 211, 406
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 7 cut(s) 28, 45, 62, 172, 218, 275, 332
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 2 cut(s) 185, 202
BseXI GCAGC 1 cut(s) 64
BseYI CCCAGC 2 cut(s) 257, 275
BshFI GGCC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 7 cut(s) 45, 103, 167, 245, 290, 303, 317
BsiWI CGTACG 1 cut(s) 142
BslFI GGGAC 4 cut(s) 18, 44, 307, 389
BslI CCNNNNNNNGG 7 cut(s) 28, 45, 62, 172, 218, 275, 332
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 4 cut(s) 18, 44, 307, 389
BsnI GGCC 1 cut(s) 49
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 68
Bsp119I TTCGAA 1 cut(s) 361
Bsp143I GATC 3 cut(s) 122, 236, 305
BspACI CCGC 9 cut(s) 50, 63, 79, 114, 225, 234, 269, 314, 355
BspANI GGCC 1 cut(s) 49
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 345, 405
BspPI GGATC 3 cut(s) 130, 244, 313
BspT104I TTCGAA 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 3 cut(s) 165, 211, 406
BssMI GATC 3 cut(s) 122, 236, 305
Bst2UI CCWGG 2 cut(s) 213, 408
Bst4CI ACNGT 2 cut(s) 40, 155
BstAPI GCANNNNNTGC 1 cut(s) 380
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 4 cut(s) 255, 273, 300, 397
BstF5I GGATG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 3 cut(s) 125, 239, 308
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 3 cut(s) 122, 236, 305
BstMWI GCNNNNNNNGC 5 cut(s) 231, 299, 352, 380, 419
BstNI CCWGG 2 cut(s) 213, 408
BstPAI GACNNNNGTC 1 cut(s) 63
BstSCI CCNGG 4 cut(s) 44, 165, 211, 406
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BsuRI GGCC 1 cut(s) 49
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 1 cut(s) 385
Cac8I GCNNGC 4 cut(s) 255, 273, 300, 397
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 422
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 47, 105, 403
Csp6I GTAC 2 cut(s) 143, 151
CviAII CATG 1 cut(s) 196
CviQI GTAC 2 cut(s) 143, 151
DpnI GATC 3 cut(s) 124, 238, 307
DpnII GATC 3 cut(s) 122, 236, 305
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EciI GGCGGA 2 cut(s) 249, 258
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 2 cut(s) 105, 403
Eco57I CTGAAG 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 403
EcoRI GAATTC 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 211, 406
EcoT22I ATGCAT 1 cut(s) 385
FaeI CATG 1 cut(s) 199
FaiI YATR 3 cut(s) 197, 219, 385
FaqI GGGAC 4 cut(s) 18, 44, 307, 389
FatI CATG 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 50, 53, 314
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 3 cut(s) 50, 53, 314
FspBI CTAG 1 cut(s) 414
GlaI GCGC 1 cut(s) 421
GluI GCNGC 3 cut(s) 50, 53, 314
GsaI CCCAGC 2 cut(s) 261, 279
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 1 cut(s) 49
HapII CCGG 7 cut(s) 45, 103, 167, 245, 290, 303, 317
HhaI GCGC 1 cut(s) 422
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HpaII CCGG 7 cut(s) 45, 103, 167, 245, 290, 303, 317
HphI GGTGA 2 cut(s) 167, 187
Hpy166II GTNNAC 2 cut(s) 264, 282
Hpy188I TCNGA 2 cut(s) 130, 379
Hpy188III TCNNGA 2 cut(s) 70, 196
Hpy8I GTNNAC 2 cut(s) 264, 282
Hpy99I CGWCG 2 cut(s) 44, 440
HpyAV CCTTC 3 cut(s) 103, 360, 385
HpyCH4III ACNGT 2 cut(s) 40, 155
HpyCH4IV ACGT 2 cut(s) 141, 438
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 5 cut(s) 231, 299, 352, 380, 419
HpySE526I ACGT 2 cut(s) 141, 438
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 420
Kzo9I GATC 3 cut(s) 122, 236, 305
LmnI GCTCC 6 cut(s) 116, 222, 247, 343, 410, 417
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 370, 383
MaeI CTAG 1 cut(s) 414
MaeII ACGT 2 cut(s) 141, 438
MaeIII GTNAC 2 cut(s) 181, 227
MalI GATC 3 cut(s) 124, 238, 307
MbiI CCGCTC 1 cut(s) 225
MboI GATC 3 cut(s) 122, 236, 305
MboII GAAGA 1 cut(s) 375
MflI RGATCY 1 cut(s) 305
MluCI AATT 2 cut(s) 160, 201
MnlI CCTC 5 cut(s) 163, 166, 180, 297, 394
Mph1103I ATGCAT 1 cut(s) 385
MseI TTAA 1 cut(s) 204
MspA1I CMGCKG 3 cut(s) 63, 257, 275
MspI CCGG 7 cut(s) 45, 103, 167, 245, 290, 303, 317
MspR9I CCNGG 4 cut(s) 46, 167, 213, 408
MvaI CCWGG 2 cut(s) 213, 408
MwoI GCNNNNNNNGC 5 cut(s) 231, 299, 352, 380, 419
NciI CCSGG 2 cut(s) 46, 167
NdeII GATC 3 cut(s) 122, 236, 305
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 2 cut(s) 345, 405
NsiI ATGCAT 1 cut(s) 385
NspV TTCGAA 1 cut(s) 361
PagI TCATGA 1 cut(s) 195
Pfl23II CGTACG 1 cut(s) 142
PflFI GACNNNGTC 1 cut(s) 38
PflMI CCANNNNNTGG 1 cut(s) 218
PkrI GCNGC 3 cut(s) 51, 54, 315
PpuMI RGGWCCY 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 2 cut(s) 211, 406
PspFI CCCAGC 2 cut(s) 257, 275
PspGI CCWGG 2 cut(s) 211, 406
PspLI CGTACG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 345, 405
PspPI GGNCC 3 cut(s) 47, 105, 403
PspPPI RGGWCCY 1 cut(s) 403
PsuI RGATCY 1 cut(s) 305
PsyI GACNNNGTC 1 cut(s) 38
PvuII CAGCTG 2 cut(s) 257, 275
RsaI GTAC 2 cut(s) 144, 152
RsaNI GTAC 2 cut(s) 143, 151
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 3 cut(s) 50, 53, 314
Sau3AI GATC 3 cut(s) 122, 236, 305
Sau96I GGNCC 3 cut(s) 47, 105, 403
ScrFI CCNGG 4 cut(s) 46, 167, 213, 408
SfaNI GCATC 2 cut(s) 370, 383
SfuI TTCGAA 1 cut(s) 361
SgrAI CRCCGGYG 1 cut(s) 316
SinI GGWCC 2 cut(s) 105, 403
Sse9I AATT 2 cut(s) 160, 201
SsiI CCGC 9 cut(s) 50, 63, 79, 114, 225, 234, 269, 314, 355
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 4 cut(s) 44, 165, 211, 406
TaaI ACNGT 2 cut(s) 40, 155
TaiI ACGT 2 cut(s) 144, 441
TaqI TCGA 1 cut(s) 361
TasI AATT 2 cut(s) 160, 201
TauI GCSGC 2 cut(s) 52, 316
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseI GCWGC 1 cut(s) 52
Tth111I GACNNNGTC 1 cut(s) 38
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 105, 403
XapI RAATTY 1 cut(s) 160
XspI CTAG 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.