MD04G1206500.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004748
Physical Location & Seq
Reverse (-)
29222142 .. 29228573
6432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1206500.v1.1.491

Sequence Viewer

Length: 3024 bp
ATGGCGGAGGTCGACGACGCCGAGGACTTCGGAGACTTCAAGTTCGTCACCGCCGTCGATCCCAATCCCAAGATCAACGGACGAGATTCGACCGTCTCGGACGACGATTGGGGCGACTTCGTAACCGACAGCTCGAGCCAGATCAAAGCTCAGGTCGTGCTCCCCAATGGAATCACGTACTCCCAATCACCTCCCGCCCAAATCCCCTTCGACCCTTTTGGGTCTTTCAATGTTTCTAATGGGTCGGCGCCGACCAGACCCGAGACAGAGCCAATCCGAGTTGAGACTGAGCCCGCGAAGGTTGATAAGACTCGGTGGGTGAAGCCGCAGGGAGCTTTGCCGTTGTCGTTATTTGGGGAAGAGCAGGAAGAGGAGGAGTCTAGTGCGGGCGAGTCCAGGGTTGGGGACATTGCAAACGAGGGATTTGCGAAAAAGGAGTCGAATTTGAATGTGAAAAATGTTGGGATCGATGATTTGATTACGAATTTGTACGGTCAGAATCCAAAAATCGTGTTTCGAAATGGGTCGGATTCGAATTTCGGCTCCGGCGGTCCTAATTCGACCATAAAAGGGTTAAATTTCATTGCAAATGGGATGGATTTGAAGTTTGATGCTCCGATTCCGAATGGAGATGGAAAACTTGGTGGTTTTAATTTGGGTGCGAATGGTTTTGATTTGAAATTTGATGGCGTGGGTTCGAATTCGAAGACAAGTGTGTCGAATTCGAGTTCTGGTGGTCCTACTTGGGTCAAAAACGGGTTGAATTTCAGTGCAAAAGCACTGGATTTCAAGCATGATGCTCTGGTTCCGAAAGAGAAATTGGGTGGCTTGAATTTGGATTCGAATGGTTTTGATTTGAAATTTGATGGGGTAGATTCAAATTCGAATACAAATGGGTTGAAGTTGAATTGGGAGGAAGGAAATGAGGACTTTGATGAGGAGGATGATGATGGTTGGGAGTTCAAAACTGCGGATTTGAAGGAACAAGGGATTATGGAAGTAGCAGGATCAAAAGCGGAATCAAATGCACACGTACGACCAGAAATCCAGGTTGACGGGAAGTGGCAAGCAAATACCAGAGGAAGTGGATTTAGTGGGGGGTTTTTATTTGATTTCAACCCTAAGCCTGTTACTCAAGATAATTTTTTCTTCGATCCACTTTCAATAAGCAAGCAGAACAATGCTGCGGATAAACCCAATTCTACTCCAGTTAATGGAAATCTGTGGGAGTTCAAGGATGCATTTTCAGAAACTGGACCAGAACATAAGTTGGAAGAGGCAATGGCTGCCAGTCCTGCTGGTGTTGGAGCTCATGCTCATAATGATTTTTTTGCTGCATTTTATGGGGATTCTAGCAAGTCTGGAGCAAGTAACTTTGCATTTCCTTATATCCCGAGTTCTGGTAGGAAAGATGGTGTAATCTCAGATTCACATTCCAGTGGCAAGAAAGAGGACAACGTAAAGGAGTTGAGTAGTTCTCCAGATGTTGGTTCTGATGACGATTTTTGGGAATTTAAGGATGCATTTTCCGGATCTGGATCAAAGCTTGAGGGGGAGCCAGTGGTTGCCGGTAATTCTCCCACCAGTATAAAACCATCTGCAATGGGTGTTGAAATCCAGCACAATGAGGTAACACTGGCGAATCATCGGCGAGCCTTGCCCCTGTCCATTTTTGGAGATGAAGAACCGGAGACTGATGATTCCTCAGTTCAACAAAATATTTCTACTCATACAACTGCATCCCACCAAGTAAATACCAAAAAAAGCCCTGCGTCCAATCTATCCATCACCGATCTAATATCAAGCTTGTACAGTCAAGTGGACCAGAACACTAACACAATTCATGTGCCGAAGTTAACTGATAATACTATACATACTGCCCCAACCTTTTTGGAATCTGATTTTGGTGATGATTTTGATGAAGATTCTTGGGAATTTAAGGATGCTGTTTCAAGAGATCAAAACCAAACTTCTATTGCTACCCTTGAAGATTCACCTCAAGATTCTTCTACTAAAGTCCATCTAGATAATTATGTGGATTCATATTGCAAACTGAAGGATGAAACATACTGTCTTGCACTCTACCATCTTGAAAATAAAAAGAAAGCTCAAAGTGGTGCTACTCTTTCTGGTGAAGATACAACAGTAGATACTCTGGAAGATCTGGAACAGGAAATCCAGAAATTGTACAGTGAACTGCATCAACACAATATGATCTCCGATCAATTCCAGTCAGGGAATCTTTCTTCAAGAAATACACAACTTCATGAAGTTCGTGAACTTCTACAAGATCCAAAGGTCCAAGTTTTTGAGTCAGAATATAAGTTGTCGCAAAGATTGTCGTTGGCAGAGAATGATTTGAGATCTGCCGTTGAACTTTCAATACACGCAGCATCCACATTAAGGATTCTTAGATCGGGATCAACTGAGGAACAATCTATTTACATTTCCACCTGGTCTCGAATAGTGTCCGTCTGCGCTGAAGAGTTGAAACATGGTTCTCTAATTTGGAGGCAATCATTAGAAGCGAATGTTCAGAATCAAATACTATCCGAACCTCAAGGCAAGCAGTATATCCTTTCCCTTGGAGAAATTTACAGAGTAGTTTTAGTTTTCGAAGCGTCTGCCAAACTTTACAAGTCATGGATTCTATTACATTCTTCAGACTGTTCTAGCTTTTTTTCTCTTCTAAATGAGTGCTCTACTTTATGGTCAAGTTCGGGACTCGATGAAGCTCTCAAGAGCATTTCAGATGCAGTTGATTTTAAATATGATGGGACCATTGCGGAACTACTAGATTCCATGACATATATTCATCATCTCGATGCATTTGCTCTCGAAAACCAAGTTGTTGTTAATGACCAAGAACCTATTTGTTCTCTGTCACTATTAACTGCAGGAGCCGTGCCAGGTGTTAAAATGGTGATGTGGAATGGGGAAAACTACTTGTTGAAACTGGCGAACTTATGGGCAAATTTAATTAGTCCTAATCCTCCGCAATTGCCCCACGTGCGTCATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1008

Amino Acids

110.1

Weight (kDa)

4.59

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYNRG_C PF25999 808 - 1001 2.1e-64 Synergin gamma, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 715
AccB1I GGYRCC 1 cut(s) 247
AccB7I CCANNNNNTGG 2 cut(s) 1214, 1487
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 1529
AclWI GGATC 8 cut(s) 53, 473, 1015, 1148, 1540, 1546, 2285, 2428
AcuI CTGAAG 3 cut(s) 2075, 2502, 2646
AcvI CACGTG 1 cut(s) 3010
AcyI GRCGYC 2 cut(s) 18, 248
AfaI GTAC 5 cut(s) 179, 491, 1035, 1811, 2189
AfiI CCNNNNNNNGG 6 cut(s) 402, 570, 1214, 1400, 1487, 2403
AflIII ACRYGT 1 cut(s) 1030
AjnI CCWGG 4 cut(s) 395, 1047, 2453, 2908
AjuI GAANNNNNNNTTGG 4 cut(s) 803, 835, 1887, 1919
AloI GAACNNNNNNTCC 2 cut(s) 2160, 2192
Alw21I GWGCWC 3 cut(s) 162, 1312, 2702
Alw26I GTCTC 6 cut(s) 27, 100, 257, 278, 1685, 2463
AlwI GGATC 8 cut(s) 53, 473, 1015, 1148, 1540, 1546, 2285, 2428
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 3 cut(s) 133, 260, 1393
Aor13HI TCCGGA 1 cut(s) 1529
ApeKI GCWGC 4 cut(s) 1184, 1286, 1334, 2390
ArsI GACNNNNNNTTYG 6 cut(s) 26, 58, 138, 170, 422, 454
AspLEI GCGC 2 cut(s) 250, 2480
AspS9I GGNCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
AsuHPI GGTGA 8 cut(s) 40, 180, 331, 1780, 1919, 1986, 2144, 2935
AsuII TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
AvaI CYCGRG 3 cut(s) 133, 260, 1393
AvaII GGWCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
BaeI ACNNNNGTAYC 2 cut(s) 2130, 2163
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 2 cut(s) 294, 1312
BbrPI CACGTG 1 cut(s) 3010
BbsI GAAGAC 1 cut(s) 713
Bbv12I GWGCWC 3 cut(s) 162, 1312, 2702
BbvI GCAGC 4 cut(s) 1171, 1273, 1321, 2402
BceAI ACGGC 4 cut(s) 38, 325, 2354, 2888
BcgI CGANNNNNNTGC 6 cut(s) 407, 441, 2606, 2640, 2813, 2847
BciT130I CCWGG 4 cut(s) 397, 1049, 2455, 2910
BcoDI GTCTC 6 cut(s) 27, 100, 257, 278, 1685, 2463
BfaI CTAG 5 cut(s) 381, 1353, 2024, 2673, 2795
BfmI CTRYAG 1 cut(s) 2895
BfoI RGCGCY 1 cut(s) 251
BglII AGATCT 2 cut(s) 2161, 2363
BisI GCNGC 5 cut(s) 326, 1185, 1287, 1335, 2391
BlsI GCNGC 5 cut(s) 327, 1186, 1288, 1336, 2392
Bme1390I CCNGG 4 cut(s) 397, 1049, 2455, 2910
Bme18I GGWCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
BmeT110I CYCGRG 3 cut(s) 133, 260, 1393
BmgT120I GGNCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
BmiI GGNNCC 6 cut(s) 249, 544, 807, 1557, 2779, 2902
BmrFI CCNGG 4 cut(s) 397, 1049, 2455, 2910
BpiI GAAGAC 1 cut(s) 713
BplI GAGNNNNNCTC 2 cut(s) 1462, 1494
BpmI CTGGAG 3 cut(s) 1191, 1383, 1464
Bpu10I CCTNAGC 2 cut(s) 150, 1122
Bpu14I TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
BpuEI CTTGAG 5 cut(s) 1119, 1568, 1983, 2544, 2723
Bsa29I ATCGAT 1 cut(s) 468
BsaAI YACGTR 3 cut(s) 177, 1033, 3010
BsaBI GATNNNNATC 3 cut(s) 63, 1537, 2419
BsaHI GRCGYC 2 cut(s) 18, 248
BsaI GGTCTC 1 cut(s) 2463
BsaJI CCNNGG 3 cut(s) 21, 396, 2584
BsaWI WCCGGW 2 cut(s) 1529, 1687
Bsc4I CCNNNNNNNGG 6 cut(s) 402, 570, 1214, 1400, 1487, 2403
Bse118I RCCGGY 1 cut(s) 1568
Bse3DI GCAATG 5 cut(s) 408, 582, 1287, 1608, 2781
Bse8I GATNNNNATC 3 cut(s) 63, 1537, 2419
BseAI TCCGGA 1 cut(s) 1529
BseBI CCWGG 4 cut(s) 397, 1049, 2455, 2910
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 21, 396, 2584
BseGI GGATG 8 cut(s) 600, 949, 1243, 1525, 1739, 1948, 2065, 2393
BseJI GATNNNNATC 3 cut(s) 63, 1537, 2419
BseLI CCNNNNNNNGG 6 cut(s) 402, 570, 1214, 1400, 1487, 2403
BseMI GCAATG 5 cut(s) 408, 582, 1287, 1608, 2781
BseMII CTCAG 5 cut(s) 164, 279, 1437, 1719, 2418
BseRI GAGGAG 3 cut(s) 386, 389, 953
BseXI GCAGC 4 cut(s) 1171, 1273, 1321, 2402
Bsh1236I CGCG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 247
BshVI ATCGAT 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 3 cut(s) 162, 1312, 2702
BsiHKCI CYCGRG 3 cut(s) 133, 260, 1393
BsiSI CCGG 4 cut(s) 546, 1530, 1569, 1688
BsiWI CGTACG 1 cut(s) 1033
BslFI GGGAC 3 cut(s) 419, 2736, 2791
BslI CCNNNNNNNGG 6 cut(s) 402, 570, 1214, 1400, 1487, 2403
BsmAI GTCTC 6 cut(s) 27, 100, 257, 278, 1685, 2463
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 3 cut(s) 419, 2736, 2791
Bso31I GGTCTC 1 cut(s) 2463
BsoBI CYCGRG 3 cut(s) 133, 260, 1393
Bsp119I TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
Bsp1286I GDGCHC 4 cut(s) 162, 294, 1312, 2702
Bsp13I TCCGGA 1 cut(s) 1529
Bsp1407I TGTACA 2 cut(s) 1809, 2187
BspCNI CTCAG 5 cut(s) 163, 280, 1436, 1718, 2419
BspDI ATCGAT 1 cut(s) 468
BspEI TCCGGA 1 cut(s) 1529
BspFNI CGCG 1 cut(s) 296
BspHI TCATGA 1 cut(s) 2266
BspLI GGNNCC 6 cut(s) 249, 544, 807, 1557, 2779, 2902
BspMAI CTGCAG 1 cut(s) 2899
BspPI GGATC 8 cut(s) 53, 473, 1015, 1148, 1540, 1546, 2285, 2428
BspQI GCTCTTC 1 cut(s) 354
BspT104I TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
BspT107I GGYRCC 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 2463
BsrDI GCAATG 5 cut(s) 408, 582, 1287, 1608, 2781
BsrFI RCCGGY 1 cut(s) 1568
BsrGI TGTACA 2 cut(s) 1809, 2187
BssAI RCCGGY 1 cut(s) 1568
BssECI CCNNGG 3 cut(s) 21, 396, 2584
BssNI GRCGYC 2 cut(s) 18, 248
BssT1I CCWWGG 1 cut(s) 2584
Bst2UI CCWGG 4 cut(s) 397, 1049, 2455, 2910
Bst4CI ACNGT 7 cut(s) 94, 494, 1814, 2072, 2146, 2192, 2669
Bst6I CTCTTC 5 cut(s) 354, 363, 1269, 2478, 2691
BstACI GRCGYC 2 cut(s) 18, 248
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstAUI TGTACA 2 cut(s) 1809, 2187
BstBAI YACGTR 3 cut(s) 177, 1033, 3010
BstBI TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
BstC8I GCNNGC 6 cut(s) 294, 388, 1068, 1172, 1653, 2567
BstDEI CTNAG 7 cut(s) 150, 288, 1122, 1423, 1705, 2411, 2427
BstF5I GGATG 8 cut(s) 600, 949, 1243, 1525, 1739, 1948, 2065, 2393
BstFNI CGCG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 250, 2480
BstMAI GTCTC 6 cut(s) 27, 100, 257, 278, 1685, 2463
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 4 cut(s) 1286, 1295, 1657, 3010
BstNI CCWGG 4 cut(s) 397, 1049, 2455, 2910
BstSCI CCNGG 1 cut(s) 395
BstSFI CTRYAG 1 cut(s) 2895
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 4 cut(s) 1171, 1273, 1321, 2402
BstV2I GAAGAC 1 cut(s) 713
BstX2I RGATCY 4 cut(s) 1532, 2161, 2290, 2363
BstYI RGATCY 4 cut(s) 1532, 2161, 2290, 2363
Bsu15I ATCGAT 1 cut(s) 468
BsuTUI ATCGAT 1 cut(s) 468
BtsCI GGATG 8 cut(s) 600, 949, 1243, 1525, 1739, 1948, 2065, 2393
BtsIMutI CAGTG 6 cut(s) 775, 779, 1444, 1566, 1634, 2197
Cac8I GCNNGC 6 cut(s) 294, 388, 1068, 1172, 1653, 2567
CaiI CAGNNNCTG 1 cut(s) 1253
CciI TCATGA 1 cut(s) 2266
CfoI GCGC 2 cut(s) 250, 2480
Cfr10I RCCGGY 1 cut(s) 1568
Cfr13I GGNCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
ClaI ATCGAT 1 cut(s) 468
CseI GACGC 4 cut(s) 26, 1761, 2610, 3002
CsiI ACCWGGT 1 cut(s) 2453
Csp6I GTAC 5 cut(s) 178, 490, 1034, 1810, 2188
CviAII CATG 7 cut(s) 794, 1313, 1844, 2267, 2495, 2643, 2803
CviQI GTAC 5 cut(s) 178, 490, 1034, 1810, 2188
DdeI CTNAG 7 cut(s) 150, 288, 1122, 1423, 1705, 2411, 2427
DinI GGCGCC 1 cut(s) 249
DraI TTTAAA 1 cut(s) 2767
DrdI GACNNNNNNGTC 1 cut(s) 715
DseDI GACNNNNNNGTC 1 cut(s) 715
Eam1104I CTCTTC 5 cut(s) 354, 363, 1269, 2478, 2691
EarI CTCTTC 5 cut(s) 354, 363, 1269, 2478, 2691
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 1310
Eco130I CCWWGG 1 cut(s) 2584
Eco24I GRGCYC 2 cut(s) 294, 1312
Eco31I GGTCTC 1 cut(s) 2463
Eco47I GGWCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
Eco53kI GAGCTC 1 cut(s) 1310
Eco57I CTGAAG 3 cut(s) 2075, 2502, 2646
Eco72I CACGTG 1 cut(s) 3010
Eco88I CYCGRG 3 cut(s) 133, 260, 1393
EcoICRI GAGCTC 1 cut(s) 1310
EcoRI GAATTC 2 cut(s) 700, 721
EcoRII CCWGG 4 cut(s) 395, 1047, 2453, 2908
EcoT14I CCWWGG 1 cut(s) 2584
EcoT22I ATGCAT 3 cut(s) 1243, 1525, 2830
EcoT38I GRGCYC 2 cut(s) 294, 1312
EgeI GGCGCC 1 cut(s) 249
EheI GGCGCC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 2584
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 7 cut(s) 797, 1316, 1847, 2270, 2498, 2646, 2806
FaqI GGGAC 3 cut(s) 419, 2736, 2791
FatI CATG 7 cut(s) 793, 1312, 1843, 2266, 2494, 2642, 2802
FauI CCCGC 3 cut(s) 202, 301, 379
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 5 cut(s) 326, 1185, 1287, 1335, 2391
FokI GGATG 8 cut(s) 607, 956, 1250, 1532, 1726, 1955, 2072, 2380
FriOI GRGCYC 2 cut(s) 294, 1312
Fsp4HI GCNGC 5 cut(s) 326, 1185, 1287, 1335, 2391
FspBI CTAG 5 cut(s) 381, 1353, 2024, 2673, 2795
GlaI GCGC 2 cut(s) 249, 2479
GluI GCNGC 5 cut(s) 326, 1185, 1287, 1335, 2391
GsuI CTGGAG 3 cut(s) 1191, 1383, 1464
HaeII RGCGCY 1 cut(s) 251
HapII CCGG 4 cut(s) 546, 1530, 1569, 1688
HgaI GACGC 4 cut(s) 26, 1761, 2610, 3002
HhaI GCGC 2 cut(s) 250, 2480
Hin1I GRCGYC 2 cut(s) 18, 248
Hin1II CATG 7 cut(s) 797, 1316, 1847, 2270, 2498, 2646, 2806
Hin6I GCGC 2 cut(s) 248, 2478
HinP1I GCGC 2 cut(s) 248, 2478
HincII GTYRAC 3 cut(s) 13, 1054, 1857
HindII GTYRAC 3 cut(s) 13, 1054, 1857
HindIII AAGCTT 2 cut(s) 1544, 1804
HpaI GTTAAC 1 cut(s) 1857
HpaII CCGG 4 cut(s) 546, 1530, 1569, 1688
HphI GGTGA 8 cut(s) 40, 180, 331, 1780, 1919, 1986, 2144, 2935
Hpy166II GTNNAC 6 cut(s) 13, 1054, 1822, 1857, 2195, 2279
Hpy8I GTNNAC 6 cut(s) 13, 1054, 1822, 1857, 2195, 2279
Hpy99I CGWCG 4 cut(s) 17, 20, 59, 107
HpyAV CCTTC 5 cut(s) 217, 292, 911, 973, 2050
HpyCH4III ACNGT 7 cut(s) 94, 494, 1814, 2072, 2146, 2192, 2669
HpyCH4IV ACGT 4 cut(s) 176, 1032, 1458, 3009
HpyF10VI GCNNNNNNNGC 4 cut(s) 1286, 1295, 1657, 3010
HpyF3I CTNAG 7 cut(s) 150, 288, 1122, 1423, 1705, 2411, 2427
HpySE526I ACGT 4 cut(s) 176, 1032, 1458, 3009
Hsp92I GRCGYC 2 cut(s) 18, 248
Hsp92II CATG 7 cut(s) 797, 1316, 1847, 2270, 2498, 2646, 2806
HspAI GCGC 2 cut(s) 248, 2478
KasI GGCGCC 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 1529
KspAI GTTAAC 1 cut(s) 1857
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 8 cut(s) 165, 332, 548, 619, 1307, 1364, 1555, 2900
Lsp1109I GCAGC 4 cut(s) 1171, 1273, 1321, 2402
MabI ACCWGGT 1 cut(s) 2453
MaeI CTAG 5 cut(s) 381, 1353, 2024, 2673, 2795
MaeII ACGT 4 cut(s) 176, 1032, 1458, 3009
MaeIII GTNAC 6 cut(s) 46, 121, 1129, 1370, 1630, 2883
MfeI CAATTG 1 cut(s) 2999
MflI RGATCY 4 cut(s) 1532, 2161, 2290, 2363
MhlI GDGCHC 4 cut(s) 162, 294, 1312, 2702
Mly113I GGCGCC 1 cut(s) 248
MlyI GAGTC 6 cut(s) 304, 386, 401, 446, 2321, 2718
MmeI TCCRAC 3 cut(s) 507, 1251, 1285
Mph1103I ATGCAT 3 cut(s) 1243, 1525, 2830
MroI TCCGGA 1 cut(s) 1529
MslI CAYNNNNRTG 2 cut(s) 1437, 2823
MspI CCGG 4 cut(s) 546, 1530, 1569, 1688
MspR9I CCNGG 4 cut(s) 397, 1049, 2455, 2910
MunI CAATTG 1 cut(s) 2999
MvaI CCWGG 4 cut(s) 397, 1049, 2455, 2910
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 4 cut(s) 1286, 1295, 1657, 3010
NarI GGCGCC 1 cut(s) 248
NlaIII CATG 7 cut(s) 797, 1316, 1847, 2270, 2498, 2646, 2806
NlaIV GGNNCC 6 cut(s) 249, 544, 807, 1557, 2779, 2902
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 2 cut(s) 46, 2883
NsiI ATGCAT 3 cut(s) 1243, 1525, 2830
NspV TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 1 cut(s) 2266
PciSI GCTCTTC 1 cut(s) 354
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 51, 111
Pfl23II CGTACG 1 cut(s) 1033
PflMI CCANNNNNTGG 2 cut(s) 1214, 1487
PkrI GCNGC 5 cut(s) 327, 1186, 1288, 1336, 2392
PleI GAGTC 6 cut(s) 304, 385, 400, 445, 2320, 2718
PluTI GGCGCC 1 cut(s) 251
PmaCI CACGTG 1 cut(s) 3010
PmlI CACGTG 1 cut(s) 3010
PpsI GAGTC 6 cut(s) 304, 385, 400, 445, 2320, 2718
Ppu21I YACGTR 3 cut(s) 177, 1033, 3010
Psp124BI GAGCTC 1 cut(s) 1312
Psp6I CCWGG 4 cut(s) 395, 1047, 2453, 2908
PspCI CACGTG 1 cut(s) 3010
PspGI CCWGG 4 cut(s) 395, 1047, 2453, 2908
PspLI CGTACG 1 cut(s) 1033
PspN4I GGNNCC 6 cut(s) 249, 544, 807, 1557, 2779, 2902
PspPI GGNCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
PspXI VCTCGAGB 1 cut(s) 133
PsrI GAACNNNNNNTAC 2 cut(s) 2367, 2399
PstI CTGCAG 1 cut(s) 2899
PstNI CAGNNNCTG 1 cut(s) 1253
PsuI RGATCY 4 cut(s) 1532, 2161, 2290, 2363
RsaI GTAC 5 cut(s) 179, 491, 1035, 1811, 2189
RsaNI GTAC 5 cut(s) 178, 490, 1034, 1810, 2188
RseI CAYNNNNRTG 2 cut(s) 1437, 2823
SacI GAGCTC 1 cut(s) 1312
SalI GTCGAC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 354
SatI GCNGC 5 cut(s) 326, 1185, 1287, 1335, 2391
Sau96I GGNCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
SchI GAGTC 6 cut(s) 304, 386, 401, 446, 2321, 2718
ScrFI CCNGG 4 cut(s) 397, 1049, 2455, 2910
SduI GDGCHC 4 cut(s) 162, 294, 1312, 2702
SexAI ACCWGGT 1 cut(s) 2453
SfcI CTRYAG 1 cut(s) 2895
SfoI GGCGCC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 133
SfuI TTCGAA 7 cut(s) 517, 533, 698, 704, 842, 884, 2616
SinI GGWCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
SlaI CTCGAG 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 1437, 2823
SmlI CTYRAG 6 cut(s) 133, 1134, 1547, 1998, 2559, 2738
SmoI CTYRAG 6 cut(s) 133, 1134, 1547, 1998, 2559, 2738
SspDI GGCGCC 1 cut(s) 247
SspI AATATT 1 cut(s) 1720
SspMI CTAG 5 cut(s) 381, 1353, 2024, 2673, 2795
SstI GAGCTC 1 cut(s) 1312
StyD4I CCNGG 4 cut(s) 395, 1047, 2453, 2908
StyI CCWWGG 1 cut(s) 2584
TaaI ACNGT 7 cut(s) 94, 494, 1814, 2072, 2146, 2192, 2669
TaiI ACGT 4 cut(s) 179, 1035, 1461, 3012
TatI WGTACW 2 cut(s) 1809, 2187
TauI GCSGC 1 cut(s) 328
TscAI CASTG 6 cut(s) 775, 786, 1444, 1566, 1641, 2197
TseFI GTSAC 2 cut(s) 46, 2883
TseI GCWGC 4 cut(s) 1184, 1286, 1334, 2390
Tsp45I GTSAC 2 cut(s) 46, 2883
TspGWI ACGGA 2 cut(s) 93, 2461
TspRI CASTG 6 cut(s) 775, 786, 1444, 1566, 1641, 2197
Van91I CCANNNNNTGG 2 cut(s) 1214, 1487
VpaK11BI GGWCC 6 cut(s) 551, 737, 1256, 1822, 2299, 2778
XbaI TCTAGA 1 cut(s) 2023
XhoI CTCGAG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 5 cut(s) 381, 1353, 2024, 2673, 2795
Zsp2I ATGCAT 3 cut(s) 1243, 1525, 2830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.