Rroxscaffold_6G00424960

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004748
Physical Location & Seq
Reverse (-)
46031998 .. 46037807
5810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424960.1

Sequence Viewer

Length: 2922 bp
ATGGCGGACGACGACGACGACGGCTTCGGAGACTTCACTTTCGTCTCCGCCGCCGCCGCCTTCAATCCCAATCCCAATCCCACCACCCAACGACAATCGGCCGCCTCCGACGACGATTGGGGCGATTTCGTAACCGTCGCCGCGCCTCCGATCAAAACCCCCGTCGTTAAAGGATTCGCACCCGCTCCGGCCCAAACACCGCCCGAACCGTTCGGGTTTTTCAACGTAACTAACTGCGCCGCTGCGGCGCCGTTAACGTCAGCCGGGTCGGATCCAATCCGGGTCGAGACGAAGGCCCAAGGGGTCAGTAAGACGAGGCAGTGGGAGAAGCCGATGGGAGCTTTGCCGTTGTCGTTGTTTGGGGAAGAGGAGAGAGACGAGCAGGTGAATCAGGATTTGACTGAGAATGATGGATTTGGGAAAAAGGGATCGAATTTGAATGGGAGTAGCGGTGGGATTGATGATTTGATATCGAATTTGTATGTTGAGAACGGTTCGAATCGGAATTCTGGTTCGGGTGGTATTGATTTTAGCAAGAGCGGGTTGAATTTGAGGTCGAATGGGGTGGATTCGAAGCTTGGTGGTTTGATTCCGAGCCAAAATGGGAAGCTGGATGGTGGTGATTTGAGTTCGAATGGTTTGAAATTTTTTGAGGGTGTAGATTTGAATGGGAGCTCAAATTCAGGTGTGTTGAAGATGAATGTGGAAGAAGGGAATGGGGATTTTAATGGGGATGATGATGATGATGATGGTTGGGAATTCAAAGCTGCAGAACCAGAGAACCGGGTTGGCAATGAGAATTCTAAGGAACGAGGAATTTTGGAAGTAGCAGAGTCAAAAGCAGAATCAAGTACACTTGTAAACGGGGGAATACAGGCGGGAACATACTGGAGGAACTGGATTTTCTGGGGGTTTGGTATTCATGAGTTTAATAATGTGTCTGTTGCATCTAATGGAACTTCTCATGGAAATGGTGAATGGGGTTTTTCATTTGCCTTCAAGTCAAGCCCTGTAACTCAAGATAATTTCTTCTCGGAAACATACTTGAAAAGCAAGCAGAGCAATGCTGCAACTGTATCCAATTCTTCCGGAGTTGATGATAATGTGTGGGGATTCAAGGATGCACTTTCGGGAACTGGATCAGAACATAAGTCGGAAGATGCTAAGGCTGCTACTCCTGCTGGTTTAGAATTGCATACATTAGATGGTGCCGGTGCTGGTCCTCGTAATGATTTTTTTGCTGGGTCAGATTGGATTTCTCACCAGTCTTCTGAAAGTAACTTTGCATTTCCTTTCATCCCAAATTCTGGCAATAAAGATGGTGTAATCTCAGAGTCCTATTCCAGTAGCAAGAAAGATGACATAGTAACAGGATTGAGTTTTCCTCCAGATAATGACCATGTTGAGTCAAAAGACAATTTTTGGGAATTTAAGGATGCATTTTCAGAATATGGATCTAAGCTTGAGGGGGAGTTAGTGGTTGCCAGTAATTCTCCTACCAATATAAAACCATCAGCAATGGCCGGAGAAAATCAGCACAGTGAGGTAACATTGAATAATCATCGGCAAGCTTTACCCTTGTCCATTTTTGGAGACGAAGAACTAGAAACTGATGATTCCTCAGTCCATCAAGATATTTCTACTCAGACAGCTGCTTCCCACCAGATAAATACTGCCAAAAGCCCTGCTACCAATGTATCCATCACTGATCTCATATCAAGCTTATACAGTCAAGTGGAACAGAACACTTCTAAACTTCATACGCCAAAAGAAACTGAAAGTACTATGCATCCTGTGACGACAGTGTTAGACTCTGATTTTGTTGTTGATGATGCTGATGATGACTCCTGGGAGTTTAAGGATGCTGTTTCAAAAGATCGAGACCCAACTTCTATTGCCAATCTTGAAGATTCACTTAAACATTCTTGTACTAAAATCCAGCTGGATGACTTTGTGGAATTGTATTGCAAGTTGAAGAATGAATCATATTTTCTTGCACTCTACCATCTTGACCAGAAAAAGAAAGCTCAAAGTAGTGCTTCTCTTTCTGGTGAGGATGCGACAGAGGCTCTGGATAAGGAAATCCAGAAACTTTATAATGAACCGCCTCAAGACAACATGAGATTAGATCAGTTCCAGTCAGGGAATCCTTCACCAAGAAATGAATGCCTTACTGAGCTTCATAAAGCTCTGCAGGAACTGAAGTTCCAAGTCCTTGAGTCAGAATATCAATTGTCACAAAGATTGTCCTTGGCAGAGAAGGATTTGATATCGGCAATTGAACTTCTAAGACATGCAGCAGCCATATTAAGGATTCTTAGACTCGGATCAGCTGAGGAGCGATCTAGGTACATTGCTGCATGGTCTCAAATGGTCTCCATATGTGCTGAAGAGCTGAAACATGGCTCTTTGATTTGGATGCAATCATTAGAGAAAAATGTTCAGAAGCAGATGTTATCTGAACCTCGAGGCAAGCAGTATTTTGTTGCTCTGGCAGAAATTTACCGGGTAGTTTTAGTTCTTGGAGCCTCAATTAAACTATACAGCCCTTGGATTCTATTACAGTCCTCAGATTGTTCTAGCTTGTTTGGTCTTCTAAACGAGTGTTCTACTCTATGGTCAAGTTCAGGACTTGATGAAGCTCTCAGAAGTATTTCAGATGTTGATGATTTCAAATATGACGGAAATGTTAATGCATTATTGGATTCCTTAACGTCTATTAATCATCTCGACACATCTTCACTCCAAAACCATTTTTTGAGTGGACAACAAGCTATATGTTCGCTGTCACTTTTAACTGCAGGAGCTGTGCCAGGTATTAAAATGGTGGCGTGGAATGAGGTACACTATTTATTGACACTCGCAAACTTGTGGATAAATTTAATTGGTGGTGAACCCCACGGTTGCTGCACTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

973

Amino Acids

105.62

Weight (kDa)

4.56

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYNRG_C PF25999 778 - 965 4.4e-58 Synergin gamma, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2097
AarI CACCTGC 1 cut(s) 373
AbsI CCTCGAGG 1 cut(s) 2466
Acc36I ACCTGC 1 cut(s) 373
AccB1I GGYRCC 2 cut(s) 247, 1208
AccB7I CCANNNNNTGG 1 cut(s) 1307
AccBSI CCGCTC 2 cut(s) 185, 540
AccII CGCG 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 1088
AclWI GGATC 6 cut(s) 266, 279, 436, 1147, 1462, 2335
AcoI YGGCCR 2 cut(s) 99, 1521
AcuI CTGAAG 3 cut(s) 2222, 2409, 2898
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 5 cut(s) 853, 1783, 1930, 2351, 2844
AfiI CCNNNNNNNGG 2 cut(s) 1307, 2310
AjnI CCWGG 2 cut(s) 1847, 2812
AloI GAACNNNNNNTCC 6 cut(s) 496, 528, 2189, 2190, 2221, 2222
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 8 cut(s) 24, 49, 281, 369, 1587, 1875, 2370, 2380
AlwI GGATC 6 cut(s) 266, 279, 436, 1147, 1462, 2335
AlwNI CAGNNNCTG 2 cut(s) 2200, 2807
Ama87I CYCGRG 1 cut(s) 2466
Aor13HI TCCGGA 1 cut(s) 1088
AoxI GGCC 4 cut(s) 99, 189, 294, 1521
ApeKI GCWGC 9 cut(s) 242, 767, 1067, 1169, 1652, 2297, 2300, 2357, 2907
ArsI GACNNNNNNTTYG 8 cut(s) 8, 23, 40, 55, 147, 179, 1802, 1834
AseI ATTAAT 1 cut(s) 2721
Asp700I GAANNNNTTC 1 cut(s) 2653
AspLEI GCGC 3 cut(s) 145, 239, 250
AspS9I GGNCC 3 cut(s) 190, 295, 1220
AsuC2I CCSGG 4 cut(s) 265, 281, 785, 2507
AsuHPI GGTGA 7 cut(s) 397, 632, 986, 1253, 2063, 2145, 2903
AsuII TTCGAA 3 cut(s) 497, 572, 632
AvaI CYCGRG 1 cut(s) 2466
AvaII GGWCC 1 cut(s) 1220
BaeI ACNNNNGTAYC 2 cut(s) 1680, 1713
BamHI GGATCC 1 cut(s) 271
BanI GGYRCC 2 cut(s) 247, 1208
BanII GRGCYC 1 cut(s) 677
BbsI GAAGAC 2 cut(s) 1260, 2585
Bbv12I GWGCWC 1 cut(s) 677
BbvCI CCTCAGC 1 cut(s) 2334
BbvI GCAGC 9 cut(s) 229, 754, 1054, 1156, 1639, 2309, 2312, 2344, 2894
BceAI ACGGC 3 cut(s) 37, 235, 331
BciT130I CCWGG 2 cut(s) 1849, 2814
BciVI GTATCC 2 cut(s) 1087, 1708
BcnI CCSGG 4 cut(s) 265, 281, 785, 2507
BcoDI GTCTC 8 cut(s) 24, 49, 281, 369, 1587, 1875, 2370, 2380
BfaI CTAG 3 cut(s) 1604, 2346, 2580
BfmI CTRYAG 3 cut(s) 768, 2192, 2799
BfoI RGCGCY 1 cut(s) 251
BfuAI ACCTGC 1 cut(s) 373
BfuI GTATCC 2 cut(s) 1087, 1708
BglI GCCNNNNNGGC 1 cut(s) 245
BmcAI AGTACT 1 cut(s) 1783
Bme1390I CCNGG 6 cut(s) 265, 281, 785, 1849, 2507, 2814
Bme18I GGWCC 1 cut(s) 1220
BmeT110I CYCGRG 1 cut(s) 2466
BmgT120I GGNCC 3 cut(s) 190, 295, 1220
BmiI GGNNCC 4 cut(s) 249, 273, 1210, 2527
BmrFI CCNGG 6 cut(s) 265, 281, 785, 1849, 2507, 2814
BmsI GCATC 9 cut(s) 956, 1111, 1150, 1426, 1798, 1822, 1852, 2047, 2409
BpiI GAAGAC 2 cut(s) 1260, 2585
BplI GAGNNNNNCTC 2 cut(s) 1369, 1401
BpmI CTGGAG 2 cut(s) 910, 1371
Bpu10I CCTNAGC 2 cut(s) 1164, 2334
Bpu14I TTCGAA 3 cut(s) 497, 572, 632
BpuEI CTTGAG 4 cut(s) 1002, 1484, 2094, 2237
BpuMI CCSGG 4 cut(s) 265, 281, 785, 2507
BsaHI GRCGYC 1 cut(s) 248
BsaI GGTCTC 3 cut(s) 1875, 2370, 2380
BsaJI CCNNGG 5 cut(s) 298, 1848, 2250, 2549, 2899
BsaWI WCCGGW 1 cut(s) 1088
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 2 cut(s) 1307, 2310
Bse118I RCCGGY 1 cut(s) 1211
Bse1I ACTGG 7 cut(s) 893, 902, 1141, 1264, 1344, 1485, 2137
Bse3DI GCAATG 4 cut(s) 799, 1069, 1524, 2352
BseAI TCCGGA 1 cut(s) 1088
BseBI CCWGG 2 cut(s) 1849, 2814
BseDI CCNNGG 5 cut(s) 298, 1848, 2250, 2549, 2899
BseLI CCNNNNNNNGG 2 cut(s) 1307, 2310
BseMI GCAATG 4 cut(s) 799, 1069, 1524, 2352
BseMII CTCAG 8 cut(s) 393, 1344, 1635, 1658, 2166, 2325, 2583, 2659
BseNI ACTGG 7 cut(s) 893, 902, 1141, 1264, 1344, 1485, 2137
BseRI GAGGAG 2 cut(s) 383, 2351
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 9 cut(s) 229, 754, 1054, 1156, 1639, 2309, 2312, 2344, 2894
BseYI CCCAGC 1 cut(s) 1241
BsgI GTGCAG 1 cut(s) 2893
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 4 cut(s) 101, 191, 296, 1523
BshNI GGYRCC 2 cut(s) 247, 1208
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 2466
BsiSI CCGG 8 cut(s) 188, 264, 280, 784, 1089, 1212, 1524, 2506
BslI CCNNNNNNNGG 2 cut(s) 1307, 2310
BsmAI GTCTC 8 cut(s) 24, 49, 281, 369, 1587, 1875, 2370, 2380
BsmBI CGTCTC 4 cut(s) 49, 281, 369, 1587
BsmI GAATGC 1 cut(s) 2171
BsnI GGCC 4 cut(s) 101, 191, 296, 1523
Bso31I GGTCTC 3 cut(s) 1875, 2370, 2380
BsoBI CYCGRG 1 cut(s) 2466
Bsp119I TTCGAA 3 cut(s) 497, 572, 632
Bsp1286I GDGCHC 1 cut(s) 677
Bsp13I TCCGGA 1 cut(s) 1088
BspANI GGCC 4 cut(s) 101, 191, 296, 1523
BspCNI CTCAG 8 cut(s) 394, 1343, 1634, 1657, 2167, 2326, 2582, 2658
BspEI TCCGGA 1 cut(s) 1088
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 4 cut(s) 249, 273, 1210, 2527
BspMAI CTGCAG 3 cut(s) 772, 2196, 2803
BspMI ACCTGC 1 cut(s) 373
BspPI GGATC 6 cut(s) 266, 279, 436, 1147, 1462, 2335
BspQI GCTCTTC 1 cut(s) 2385
BspT104I TTCGAA 3 cut(s) 497, 572, 632
BspT107I GGYRCC 2 cut(s) 247, 1208
BspTNI GGTCTC 3 cut(s) 1875, 2370, 2380
BsrBI CCGCTC 2 cut(s) 185, 540
BsrDI GCAATG 4 cut(s) 799, 1069, 1524, 2352
BsrFI RCCGGY 1 cut(s) 1211
BsrI ACTGG 7 cut(s) 893, 902, 1141, 1264, 1344, 1485, 2137
BssAI RCCGGY 1 cut(s) 1211
BssECI CCNNGG 5 cut(s) 298, 1848, 2250, 2549, 2899
BssNI GRCGYC 1 cut(s) 248
BssT1I CCWWGG 3 cut(s) 298, 2250, 2549
Bst2UI CCWGG 2 cut(s) 1849, 2814
Bst4CI ACNGT 9 cut(s) 136, 210, 494, 1075, 1541, 1730, 1804, 2565, 2903
Bst6I CTCTTC 2 cut(s) 360, 2385
BstACI GRCGYC 1 cut(s) 248
BstBI TTCGAA 3 cut(s) 497, 572, 632
BstC8I GCNNGC 3 cut(s) 1055, 1569, 2474
BstDSI CCRYGG 1 cut(s) 2899
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 3 cut(s) 145, 239, 250
BstMAI GTCTC 8 cut(s) 24, 49, 281, 369, 1587, 1875, 2370, 2380
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 6 cut(s) 56, 245, 1059, 1169, 1178, 2066
BstNI CCWGG 2 cut(s) 1849, 2814
BstNSI RCATGY 1 cut(s) 2297
BstSCI CCNGG 6 cut(s) 263, 279, 783, 1847, 2505, 2812
BstSFI CTRYAG 3 cut(s) 768, 2192, 2799
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 9 cut(s) 229, 754, 1054, 1156, 1639, 2309, 2312, 2344, 2894
BstV2I GAAGAC 2 cut(s) 1260, 2585
BstX2I RGATCY 2 cut(s) 271, 1454
BstYI RGATCY 2 cut(s) 271, 1454
BstZI CGGCCG 1 cut(s) 99
BsuI GTATCC 2 cut(s) 1087, 1708
BsuRI GGCC 4 cut(s) 101, 191, 296, 1523
BtgI CCRYGG 1 cut(s) 2899
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 4 cut(s) 326, 1546, 1704, 1809
BveI ACCTGC 1 cut(s) 373
Cac8I GCNNGC 3 cut(s) 1055, 1569, 2474
CaiI CAGNNNCTG 2 cut(s) 2200, 2807
CciI TCATGA 1 cut(s) 922
CfoI GCGC 3 cut(s) 145, 239, 250
Cfr10I RCCGGY 1 cut(s) 1211
Cfr13I GGNCC 3 cut(s) 190, 295, 1220
Csp6I GTAC 5 cut(s) 852, 1782, 1929, 2350, 2843
CviAII CATG 7 cut(s) 923, 965, 1400, 2119, 2294, 2361, 2402
CviQI GTAC 5 cut(s) 852, 1782, 1929, 2350, 2843
DinI GGCGCC 1 cut(s) 249
EaeI YGGCCR 2 cut(s) 99, 1521
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 360, 2385
EarI CTCTTC 2 cut(s) 360, 2385
EciI GGCGGA 2 cut(s) 20, 37
Ecl136II GAGCTC 1 cut(s) 675
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 3 cut(s) 298, 2250, 2549
Eco24I GRGCYC 1 cut(s) 677
Eco31I GGTCTC 3 cut(s) 1875, 2370, 2380
Eco32I GATATC 2 cut(s) 471, 2271
Eco47I GGWCC 1 cut(s) 1220
Eco52I CGGCCG 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 3 cut(s) 2222, 2409, 2898
Eco88I CYCGRG 1 cut(s) 2466
EcoICRI GAGCTC 1 cut(s) 675
EcoRI GAATTC 3 cut(s) 505, 758, 799
EcoRII CCWGG 2 cut(s) 1847, 2812
EcoRV GATATC 2 cut(s) 471, 2271
EcoT14I CCWWGG 3 cut(s) 298, 2250, 2549
EcoT22I ATGCAT 3 cut(s) 1441, 1791, 2698
EcoT38I GRGCYC 1 cut(s) 677
EgeI GGCGCC 1 cut(s) 249
EheI GGCGCC 1 cut(s) 249
ErhI CCWWGG 3 cut(s) 298, 2250, 2549
Esp3I CGTCTC 4 cut(s) 49, 281, 369, 1587
FaeI CATG 7 cut(s) 926, 968, 1403, 2122, 2297, 2364, 2405
FalI AAGNNNNNCTT 4 cut(s) 1899, 1931, 2023, 2055
FatI CATG 7 cut(s) 922, 964, 1399, 2118, 2293, 2360, 2401
FauI CCCGC 3 cut(s) 190, 533, 871
FauNDI CATATG 1 cut(s) 2381
FriOI GRGCYC 1 cut(s) 677
FspBI CTAG 3 cut(s) 1604, 2346, 2580
GlaI GCGC 3 cut(s) 144, 238, 249
GsaI CCCAGC 1 cut(s) 1245
GsuI CTGGAG 2 cut(s) 910, 1371
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 4 cut(s) 101, 191, 296, 1523
HapII CCGG 8 cut(s) 188, 264, 280, 784, 1089, 1212, 1524, 2506
HhaI GCGC 3 cut(s) 145, 239, 250
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 7 cut(s) 926, 968, 1403, 2122, 2297, 2364, 2405
Hin6I GCGC 3 cut(s) 143, 237, 248
HinP1I GCGC 3 cut(s) 143, 237, 248
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HindIII AAGCTT 4 cut(s) 575, 1460, 1569, 1720
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 8 cut(s) 188, 264, 280, 784, 1089, 1212, 1524, 2506
HphI GGTGA 7 cut(s) 397, 632, 986, 1253, 2063, 2145, 2903
Hpy166II GTNNAC 6 cut(s) 255, 854, 862, 2765, 2845, 2894
Hpy8I GTNNAC 6 cut(s) 255, 854, 862, 2765, 2845, 2894
Hpy99I CGWCG 8 cut(s) 14, 17, 20, 23, 113, 116, 140, 167
HpyAV CCTTC 6 cut(s) 70, 286, 704, 1006, 2160, 2254
HpyCH4III ACNGT 9 cut(s) 136, 210, 494, 1075, 1541, 1730, 1804, 2565, 2903
HpyCH4IV ACGT 3 cut(s) 225, 257, 2714
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 245, 1059, 1169, 1178, 2066
HpySE526I ACGT 3 cut(s) 225, 257, 2714
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 7 cut(s) 926, 968, 1403, 2122, 2297, 2364, 2405
HspAI GCGC 3 cut(s) 143, 237, 248
KasI GGCGCC 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 1088
KspAI GTTAAC 1 cut(s) 255
LguI GCTCTTC 1 cut(s) 2385
LmnI GCTCC 6 cut(s) 190, 338, 672, 2338, 2525, 2804
Lsp1109I GCAGC 9 cut(s) 229, 754, 1054, 1156, 1639, 2309, 2312, 2344, 2894
LweI GCATC 9 cut(s) 956, 1111, 1150, 1426, 1798, 1822, 1852, 2047, 2409
MaeI CTAG 3 cut(s) 1604, 2346, 2580
MaeII ACGT 3 cut(s) 225, 257, 2714
MaeIII GTNAC 9 cut(s) 130, 226, 1012, 1277, 1366, 1546, 1795, 2235, 2787
MbiI CCGCTC 2 cut(s) 185, 540
MfeI CAATTG 2 cut(s) 2231, 2277
MflI RGATCY 2 cut(s) 271, 1454
MhlI GDGCHC 1 cut(s) 677
Mly113I GGCGCC 1 cut(s) 248
MlyI GAGTC 7 cut(s) 842, 1343, 1415, 1805, 1838, 2228, 2316
MmeI TCCRAC 3 cut(s) 132, 249, 1134
Mph1103I ATGCAT 3 cut(s) 1441, 1791, 2698
MroI TCCGGA 1 cut(s) 1088
MroXI GAANNNNTTC 1 cut(s) 2653
MslI CAYNNNNRTG 1 cut(s) 969
MspA1I CMGCKG 4 cut(s) 242, 1652, 1942, 2333
MspI CCGG 8 cut(s) 188, 264, 280, 784, 1089, 1212, 1524, 2506
MspR9I CCNGG 6 cut(s) 265, 281, 785, 1849, 2507, 2814
MunI CAATTG 2 cut(s) 2231, 2277
Mva1269I GAATGC 1 cut(s) 2171
MvaI CCWGG 2 cut(s) 1849, 2814
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 6 cut(s) 56, 245, 1059, 1169, 1178, 2066
NarI GGCGCC 1 cut(s) 248
NciI CCSGG 4 cut(s) 265, 281, 785, 2507
NdeI CATATG 1 cut(s) 2381
NlaIII CATG 7 cut(s) 926, 968, 1403, 2122, 2297, 2364, 2405
NlaIV GGNNCC 4 cut(s) 249, 273, 1210, 2527
NmuCI GTSAC 3 cut(s) 1795, 2235, 2787
NsiI ATGCAT 3 cut(s) 1441, 1791, 2698
NspI RCATGY 1 cut(s) 2297
NspV TTCGAA 3 cut(s) 497, 572, 632
PaeR7I CTCGAG 1 cut(s) 2466
PagI TCATGA 1 cut(s) 922
PaqCI CACCTGC 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 2385
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 120
PctI GAATGC 1 cut(s) 2171
PdmI GAANNNNTTC 1 cut(s) 2653
PflMI CCANNNNNTGG 1 cut(s) 1307
PleI GAGTC 7 cut(s) 841, 1342, 1414, 1805, 1838, 2227, 2316
PluTI GGCGCC 1 cut(s) 251
PpsI GAGTC 7 cut(s) 841, 1342, 1414, 1805, 1838, 2227, 2316
PshBI ATTAAT 1 cut(s) 2721
PsiI TTATAA 1 cut(s) 2097
Psp124BI GAGCTC 1 cut(s) 677
Psp6I CCWGG 2 cut(s) 1847, 2812
PspFI CCCAGC 1 cut(s) 1241
PspGI CCWGG 2 cut(s) 1847, 2812
PspN4I GGNNCC 4 cut(s) 249, 273, 1210, 2527
PspPI GGNCC 3 cut(s) 190, 295, 1220
PspXI VCTCGAGB 1 cut(s) 2466
PsrI GAACNNNNNNTAC 2 cut(s) 2502, 2534
PstI CTGCAG 3 cut(s) 772, 2196, 2803
PstNI CAGNNNCTG 2 cut(s) 2200, 2807
PsuI RGATCY 2 cut(s) 271, 1454
PvuII CAGCTG 3 cut(s) 1652, 1942, 2333
RsaI GTAC 5 cut(s) 853, 1783, 1930, 2351, 2844
RsaNI GTAC 5 cut(s) 852, 1782, 1929, 2350, 2843
RseI CAYNNNNRTG 1 cut(s) 969
SacI GAGCTC 1 cut(s) 677
SapI GCTCTTC 1 cut(s) 2385
Sau96I GGNCC 3 cut(s) 190, 295, 1220
ScaI AGTACT 1 cut(s) 1783
SchI GAGTC 7 cut(s) 842, 1343, 1415, 1805, 1838, 2228, 2316
ScrFI CCNGG 6 cut(s) 265, 281, 785, 1849, 2507, 2814
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 9 cut(s) 956, 1111, 1150, 1426, 1798, 1822, 1852, 2047, 2409
SfcI CTRYAG 3 cut(s) 768, 2192, 2799
SfoI GGCGCC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 2466
SfuI TTCGAA 3 cut(s) 497, 572, 632
SinI GGWCC 1 cut(s) 1220
SlaI CTCGAG 1 cut(s) 2466
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 5 cut(s) 1017, 1463, 2109, 2216, 2466
SmoI CTYRAG 5 cut(s) 1017, 1463, 2109, 2216, 2466
SspDI GGCGCC 1 cut(s) 247
SspMI CTAG 3 cut(s) 1604, 2346, 2580
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 6 cut(s) 263, 279, 783, 1847, 2505, 2812
StyI CCWWGG 3 cut(s) 298, 2250, 2549
TaaI ACNGT 9 cut(s) 136, 210, 494, 1075, 1541, 1730, 1804, 2565, 2903
TaiI ACGT 3 cut(s) 228, 260, 2717
TatI WGTACW 3 cut(s) 851, 1781, 1928
TauI GCSGC 7 cut(s) 53, 56, 59, 104, 143, 242, 248
TscAI CASTG 4 cut(s) 326, 1546, 1711, 1809
TseFI GTSAC 3 cut(s) 1795, 2235, 2787
TseI GCWGC 9 cut(s) 242, 767, 1067, 1169, 1652, 2297, 2300, 2357, 2907
Tsp45I GTSAC 3 cut(s) 1795, 2235, 2787
TspGWI ACGGA 1 cut(s) 2697
TspRI CASTG 4 cut(s) 326, 1546, 1711, 1809
Van91I CCANNNNNTGG 1 cut(s) 1307
VpaK11BI GGWCC 1 cut(s) 1220
VspI ATTAAT 1 cut(s) 2721
XceI RCATGY 1 cut(s) 2297
XcmI CCANNNNNNNNNTGG 1 cut(s) 2759
XhoI CTCGAG 1 cut(s) 2466
XmnI GAANNNNTTC 1 cut(s) 2653
XspI CTAG 3 cut(s) 1604, 2346, 2580
ZrmI AGTACT 1 cut(s) 1783
Zsp2I ATGCAT 3 cut(s) 1441, 1791, 2698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.