MD12G1220300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004748
Physical Location & Seq
Reverse (-)
29718490 .. 29724735
6246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1220300.v1.1.491

Sequence Viewer

Length: 3009 bp
ATGGCGGAGGTCGACGACGCCGGGGGCTTCGGAGACTTCAAGTTCGTCACCGCCGTCGATCCCAAGCCCAAGATCAACGGACGAGATTCGACCGTCTCGGACGACGATTGGGGCGACTTCGTAACGGACAGCACGAGTCAGATCAAAGCTCAGGCCGTGCTCTCCAATGGAATCACGTACTCCCAGTCACCTCCCTCCCAAATACCCGTCGACCCTTTGGGGTTTTTCGTTCCTAATGGGTCGGCGCCGACCCGAACGGAATCAGAGCCAATCCAAGTTGAGACTGAGCCCGCGAAGGCTGATAAGACTCGGTGGGTAAAGCCGCAGGGAGCTTTGCCGTTATCGTTATTTTGGGAAGAGCAGGAAGAGGACTCCGGTGCGGGTGAGTCCAAGGTTGGGGACGTTGCAAACGAGGGATTTGCGAAAAAGGAGTCGAATTTGAATGTTGAGATCAATGATTTGATTGCAAATTTGTACGGTCATAATCCAAAAGTCGTGGTTCGAAATGGGTCGGATTCGAATTTCGGTCCTGGAGGTCCCAATTCAACAAAAAAGGGGTTGGATTTCAGTGCAAATGGGATGGATTTGAAGTTTGATGCTCCCATTCCGAACGGAAATGGGAACTTTGGTGGTTTGAATTTTGTTTCGAATGGTTTTGATATGAAATTTGACGATGTGGGTTCGAATTCAAATACAAGTGTGTCGAATTCGGGTTCTGGTGGTCCTCATACGGTAAAAAACGGATTGAATTTCAGTGCAGATGCATTGGATTTGAAGCTTGATGCTCTGGTTCCGAAAGAAAATGGGACATCGGGTGGCTTGAATTTGGATTCGAATGGTTTTGATTTGAAATTTGATGGGGTAGATTCGAATTCAAATACACTTGGGTTGAGGTTGGATTTGGAGGAAGGAAATGGGGACTTTGATGAGGAGGATGAGGATGGTTGGGAATTCAAAGCTGCGGATTTGAAGGAACAAGGGGTTGTGGAAGTAGCAGGATCGAAAGCGGAATCAAGTGCACACGCACGACCAGGAATCCAGGTTGATGAGAAGTGGCAAGCAAATACTGGAGGAAGTGGATTTAGTGGGGGGTTTTCATTTGATTTCAACCCAAAGCCTGTTACTCAACATAATTTTTTCTTCGATCCACTTTTGAAAAGCAAGCAGAACAGTGCTGCGGATATTCCCAATTCTACTCCAGTTAATGGAAATCTGTGGGAGTTCAAGGATGCATTTTCAGAAACTGGAGCAAAACATAAGTTGGAAGAGGCAAGGGCTGCCAATCCTGCTGGTTTTGTCTCCCGTGCTCATAATGATTTTTTTGCTGCATTTCATGGGGATCCCCACAAGTCTGGAGAAAACTTTGCATTTCCTTTTATCCCGACTTCTAGCAGGAAACATGGTGTAATCTCAGATTCACATTCCAGTGGCAAGAAAGAGGACATTGAAAAAGATTTGAGTAGTTCTCCAGATGTTGGGTCTGATGACAATTTTTGGGAATTTAAGGATGCATTTTCCGAATCTGGATCAAATATTGAGGGGGAGTTAGTGGTTGGCGGTAATCCTCCCGCCAATATAAAACCATATGTCATGGGTTTTGAAATCCAGCATAATGAGGTAACATTGGAAGAAAATCATCGACGAGCCTTGCCCTTGTCCATTTTTGGAGATGATGAACTAGAGACTGATGATTCCTCAATTCAACAAGATATTTCTTCTCTTACCACTGCATCCCACCAAGTAAATACCAACAAAAGCCCTGCGTCCAATTTATCCATCACGGATCTAATATCAAGCTTATACAGTCAAGTGGACCAGAACACTAACACAATTCATGCGCCGAAACCAACTGAAAGTATTATGGATCCTGCCCCAACCGTTTTGGAATCTAATTTTGGTGGTGATGATTTTGATGAAGATTCTTGGGAATTTAAGGATGCTGTTTCAAGTGATCAAAACCAAACCTATATTGCTACTCTTGAAGATTCACCTCACGATTCTTCTGCTAAAGTCCATCTTGATAATTATGTGGATTTATATTGCAAATTGAAGGATGAAACATACGGTCTTGCACTCTACCATCTTGAAAATAAAAAGAAAGCTCAAAGTGGTGCTACTCTTTCTGGTGAAGATACAACAATAGAGACTATGGAAGAGGAAATCCAGAAATTGTACAATGAACTGCATCAACACAATATGATCTCCGATCAATTCTGGTCAGGGAATCTTTCATCAAGAAATACACACATTCAAGAAGTTCGTAAACTTCTACAGGATCCAAAGTTCCAAGTTCTTGAATCCGAATACCAGTTGTCACAAAGGTTGTCATTGGCAGAGAATGATTTGAGATCTGCTGTTGAACTTTCAAGACACGCGGCATCCATGTTAAGGATTCTTAGATTGGGATCAACTGAGGAACAATCTAACTTCATTTCCACCTGGTCTCGAATGGTCTCCTTTTGCGCTGAAGAGTTGAAACATGGTTCTCTAATTTGGAGGCAATCATTAGAAGCGAATGTTCAGAATCAAATACTATCTGAACCTCAAGGCAAGCAGTATATTGTTGCCCTTGGAGAAATTTACAGAGTAGTTTTAGTTCTTGAAGCGTCTGCCAAACTTTACAAGTCATGGATTCTATTACATTCTTCAAACTGTTTTAGTTTTTTTTCTCTTCTGAACGAGTGCTCTACTTTATGGTCAAGTTCGGGACTTGATGAAGCTCTCAAGAGCATTTCGGATGCGATTGATTTTAAATATGACGAGACCGTTGCTGCACTACTAGATTCCATGACATACATTCATCATCTGGATGCATTTGCTCTCCAAAACCAAGTTGTTGTATATGGCCAAGAACCTATTTGTTCGCTAACACTATTAACTGCAGGAGCCGTGCCAGGTATTAAAATGGTGATGTGGAATGGGGAACACTACTTATTGACGCTTGCGAACTTGTGGGCAAATTTAATTAGTCCTAGTCCTCCGGAATTGCCCCACTTGAGTTATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1003

Amino Acids

109.53

Weight (kDa)

4.58

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYNRG_C PF25999 803 - 996 8.7e-64 Synergin gamma, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AccB7I CCANNNNNTGG 2 cut(s) 1205, 1475
AccI GTMKAC 2 cut(s) 12, 210
AccII CGCG 2 cut(s) 293, 2375
AccIII TCCGGA 1 cut(s) 2980
AcoI YGGCCR 1 cut(s) 2845
AcuI CTGAAG 1 cut(s) 2487
AcyI GRCGYC 2 cut(s) 18, 245
AfaI GTAC 3 cut(s) 179, 476, 2174
AfiI CCNNNNNNNGG 4 cut(s) 396, 1205, 1475, 2388
AjnI CCWGG 5 cut(s) 529, 1030, 1038, 2438, 2893
AjuI GAANNNNNNNTTGG 2 cut(s) 1878, 1910
AloI GAACNNNNNNTCC 2 cut(s) 2267, 2299
AluBI AGCT 8 cut(s) 149, 332, 778, 959, 1797, 2102, 2720, 3006
AluI AGCT 8 cut(s) 149, 332, 778, 959, 1797, 2102, 2720, 3006
Alw21I GWGCWC 4 cut(s) 162, 1021, 1309, 2687
Alw26I GTCTC 9 cut(s) 27, 100, 275, 1303, 1676, 2138, 2448, 2458, 2756
Alw44I GTGCAC 1 cut(s) 1017
AlwNI CAGNNNCTG 1 cut(s) 1244
Aor13HI TCCGGA 1 cut(s) 2980
AoxI GGCC 2 cut(s) 153, 2845
ApaLI GTGCAC 1 cut(s) 1017
ApeKI GCWGC 5 cut(s) 959, 1175, 1277, 1325, 2771
ArsI GACNNNNNNTTYG 4 cut(s) 26, 58, 416, 448
AspLEI GCGC 3 cut(s) 247, 1840, 2465
AspS9I GGNCC 4 cut(s) 527, 536, 722, 1813
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 7 cut(s) 40, 180, 395, 1913, 1980, 2138, 2920
AsuII TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
AvaII GGWCC 4 cut(s) 527, 536, 722, 1813
BaeGI GKGCMC 1 cut(s) 1021
BalI TGGCCA 1 cut(s) 2847
BamHI GGATCC 3 cut(s) 1339, 1864, 2275
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 291
BauI CACGAG 1 cut(s) 133
Bbv12I GWGCWC 4 cut(s) 162, 1021, 1309, 2687
BbvI GCAGC 5 cut(s) 946, 1162, 1264, 1312, 2758
BccI CCATC 6 cut(s) 574, 851, 935, 1784, 2022, 2088
BceAI ACGGC 4 cut(s) 38, 140, 322, 2873
BcgI CGANNNNNNTGC 6 cut(s) 401, 435, 1985, 2019, 2750, 2784
BciT130I CCWGG 5 cut(s) 531, 1032, 1040, 2440, 2895
BclI TGATCA 1 cut(s) 1951
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 9 cut(s) 27, 100, 275, 1303, 1676, 2138, 2448, 2458, 2756
BfaI CTAG 4 cut(s) 1389, 1679, 2780, 2973
BfmI CTRYAG 2 cut(s) 2270, 2880
BfoI RGCGCY 1 cut(s) 248
BglII AGATCT 1 cut(s) 2348
BisI GCNGC 7 cut(s) 323, 960, 1176, 1278, 1326, 2376, 2772
BlsI GCNGC 7 cut(s) 324, 961, 1177, 1279, 1327, 2377, 2773
Bme1390I CCNGG 6 cut(s) 22, 531, 1032, 1040, 2440, 2895
Bme18I GGWCC 4 cut(s) 527, 536, 722, 1813
BmgT120I GGNCC 4 cut(s) 527, 536, 722, 1813
BmiI GGNNCC 7 cut(s) 246, 538, 792, 1341, 1866, 2277, 2887
BmrFI CCNGG 6 cut(s) 22, 531, 1032, 1040, 2440, 2895
BmrI ACTGGG 1 cut(s) 178
BmuI ACTGGG 1 cut(s) 178
BplI GAGNNNNNCTC 2 cut(s) 1450, 1482
BpmI CTGGAG 6 cut(s) 552, 1089, 1182, 1266, 1374, 1452
Bpu10I CCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
BpuEI CTTGAG 2 cut(s) 2529, 2708
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 2404
BsaHI GRCGYC 2 cut(s) 18, 245
BsaI GGTCTC 3 cut(s) 2448, 2458, 2756
BsaJI CCNNGG 3 cut(s) 21, 390, 2569
BsaWI WCCGGW 2 cut(s) 374, 2980
Bsc4I CCNNNNNNNGG 4 cut(s) 396, 1205, 1475, 2388
Bse1I ACTGG 6 cut(s) 184, 1072, 1199, 1249, 1425, 2308
Bse8I GATNNNNATC 1 cut(s) 2404
BseAI TCCGGA 1 cut(s) 2980
BseBI CCWGG 5 cut(s) 531, 1032, 1040, 2440, 2895
BseDI CCNNGG 3 cut(s) 21, 390, 2569
BseJI GATNNNNATC 1 cut(s) 2404
BseLI CCNNNNNNNGG 4 cut(s) 396, 1205, 1475, 2388
BseMII CTCAG 4 cut(s) 164, 276, 1425, 2403
BseNI ACTGG 6 cut(s) 184, 1072, 1199, 1249, 1425, 2308
BseRI GAGGAG 1 cut(s) 944
BseSI GKGCMC 1 cut(s) 1021
BseXI GCAGC 5 cut(s) 946, 1162, 1264, 1312, 2758
BsgI GTGCAG 2 cut(s) 777, 2757
Bsh1236I CGCG 2 cut(s) 293, 2375
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 155, 2847
BshNI GGYRCC 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 4 cut(s) 162, 1021, 1309, 2687
BsiSI CCGG 3 cut(s) 21, 375, 2981
BslFI GGGAC 5 cut(s) 413, 522, 820, 932, 2721
BslI CCNNNNNNNGG 4 cut(s) 396, 1205, 1475, 2388
BsmAI GTCTC 9 cut(s) 27, 100, 275, 1303, 1676, 2138, 2448, 2458, 2756
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 5 cut(s) 413, 522, 820, 932, 2721
BsnI GGCC 2 cut(s) 155, 2847
Bso31I GGTCTC 3 cut(s) 2448, 2458, 2756
Bsp119I TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
Bsp1286I GDGCHC 5 cut(s) 162, 291, 1021, 1309, 2687
Bsp13I TCCGGA 1 cut(s) 2980
Bsp1407I TGTACA 1 cut(s) 2172
BspANI GGCC 2 cut(s) 155, 2847
BspCNI CTCAG 4 cut(s) 163, 277, 1424, 2404
BspEI TCCGGA 1 cut(s) 2980
BspFNI CGCG 2 cut(s) 293, 2375
BspLI GGNNCC 7 cut(s) 246, 538, 792, 1341, 1866, 2277, 2887
BspMAI CTGCAG 1 cut(s) 2884
BspQI GCTCTTC 1 cut(s) 351
BspT104I TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
BspT107I GGYRCC 1 cut(s) 244
BspTNI GGTCTC 3 cut(s) 2448, 2458, 2756
BsrGI TGTACA 1 cut(s) 2172
BsrI ACTGG 6 cut(s) 184, 1072, 1199, 1249, 1425, 2308
BssECI CCNNGG 3 cut(s) 21, 390, 2569
BssNI GRCGYC 2 cut(s) 18, 245
BssSI CACGAG 1 cut(s) 133
BssT1I CCWWGG 2 cut(s) 390, 2569
Bst2BI CACGAG 1 cut(s) 133
Bst2UI CCWGG 5 cut(s) 531, 1032, 1040, 2440, 2895
Bst4CI ACNGT 9 cut(s) 94, 479, 733, 1172, 1805, 1879, 2066, 2654, 2767
Bst6I CTCTTC 6 cut(s) 351, 360, 1260, 2148, 2463, 2676
BstACI GRCGYC 2 cut(s) 18, 245
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstAUI TGTACA 1 cut(s) 2172
BstBAI YACGTR 1 cut(s) 177
BstBI TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
BstC8I GCNNGC 5 cut(s) 291, 1059, 1163, 2552, 2943
BstDEI CTNAG 5 cut(s) 150, 285, 1411, 2396, 2412
BstFNI CGCG 2 cut(s) 293, 2375
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 3 cut(s) 247, 1840, 2465
BstMAI GTCTC 9 cut(s) 27, 100, 275, 1303, 1676, 2138, 2448, 2458, 2756
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 1277, 1286
BstNI CCWGG 5 cut(s) 531, 1032, 1040, 2440, 2895
BstSCI CCNGG 6 cut(s) 20, 529, 1030, 1038, 2438, 2893
BstSFI CTRYAG 2 cut(s) 2270, 2880
BstSLI GKGCMC 1 cut(s) 1021
BstUI CGCG 2 cut(s) 293, 2375
BstV1I GCAGC 5 cut(s) 946, 1162, 1264, 1312, 2758
BstX2I RGATCY 5 cut(s) 1339, 1783, 1864, 2275, 2348
BstXI CCANNNNNNTGG 1 cut(s) 1352
BstYI RGATCY 5 cut(s) 1339, 1783, 1864, 2275, 2348
BsuRI GGCC 2 cut(s) 155, 2847
BtsI GCAGTG 1 cut(s) 1725
BtsIMutI CAGTG 5 cut(s) 574, 760, 1177, 1432, 1725
Cac8I GCNNGC 5 cut(s) 291, 1059, 1163, 2552, 2943
CaiI CAGNNNCTG 1 cut(s) 1244
CfoI GCGC 3 cut(s) 247, 1840, 2465
Cfr13I GGNCC 4 cut(s) 527, 536, 722, 1813
CseI GACGC 4 cut(s) 26, 1752, 2595, 2947
CsiI ACCWGGT 1 cut(s) 2438
Csp6I GTAC 3 cut(s) 178, 475, 2173
CspCI CAANNNNNGTGG 2 cut(s) 477, 512
CviAII CATG 8 cut(s) 1334, 1400, 1591, 1835, 2383, 2480, 2628, 2788
CviQI GTAC 3 cut(s) 178, 475, 2173
DdeI CTNAG 5 cut(s) 150, 285, 1411, 2396, 2412
DinI GGCGCC 1 cut(s) 246
DraI TTTAAA 1 cut(s) 2752
EaeI YGGCCR 1 cut(s) 2845
Eam1104I CTCTTC 6 cut(s) 351, 360, 1260, 2148, 2463, 2676
EarI CTCTTC 6 cut(s) 351, 360, 1260, 2148, 2463, 2676
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 390, 2569
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 3 cut(s) 2448, 2458, 2756
Eco47I GGWCC 4 cut(s) 527, 536, 722, 1813
Eco57I CTGAAG 1 cut(s) 2487
EcoO109I RGGNCCY 1 cut(s) 536
EcoRI GAATTC 4 cut(s) 685, 706, 871, 950
EcoRII CCWGG 5 cut(s) 529, 1030, 1038, 2438, 2893
EcoT14I CCWWGG 2 cut(s) 390, 2569
EcoT22I ATGCAT 4 cut(s) 766, 1234, 1513, 2815
EcoT38I GRGCYC 1 cut(s) 291
EgeI GGCGCC 1 cut(s) 246
EheI GGCGCC 1 cut(s) 246
ErhI CCWWGG 2 cut(s) 390, 2569
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 8 cut(s) 1337, 1403, 1594, 1838, 2386, 2483, 2631, 2791
FalI AAGNNNNNCTT 2 cut(s) 2001, 2033
FaqI GGGAC 5 cut(s) 413, 522, 820, 932, 2721
FatI CATG 8 cut(s) 1333, 1399, 1590, 1834, 2382, 2479, 2627, 2787
FauI CCCGC 3 cut(s) 298, 373, 1576
FauNDI CATATG 1 cut(s) 1585
FbaI TGATCA 1 cut(s) 1951
FblI GTMKAC 2 cut(s) 12, 210
Fnu4HI GCNGC 7 cut(s) 323, 960, 1176, 1278, 1326, 2376, 2772
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 7 cut(s) 323, 960, 1176, 1278, 1326, 2376, 2772
FspBI CTAG 4 cut(s) 1389, 1679, 2780, 2973
GlaI GCGC 3 cut(s) 246, 1839, 2464
GluI GCNGC 7 cut(s) 323, 960, 1176, 1278, 1326, 2376, 2772
GsuI CTGGAG 6 cut(s) 552, 1089, 1182, 1266, 1374, 1452
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 2 cut(s) 155, 2847
HapII CCGG 3 cut(s) 21, 375, 2981
HgaI GACGC 4 cut(s) 26, 1752, 2595, 2947
HhaI GCGC 3 cut(s) 247, 1840, 2465
Hin1I GRCGYC 2 cut(s) 18, 245
Hin1II CATG 8 cut(s) 1337, 1403, 1594, 1838, 2386, 2483, 2631, 2791
Hin6I GCGC 3 cut(s) 245, 1838, 2463
HinP1I GCGC 3 cut(s) 245, 1838, 2463
HincII GTYRAC 2 cut(s) 13, 211
HindII GTYRAC 2 cut(s) 13, 211
HindIII AAGCTT 2 cut(s) 776, 1795
HpaII CCGG 3 cut(s) 21, 375, 2981
HphI GGTGA 7 cut(s) 40, 180, 395, 1913, 1980, 2138, 2920
Hpy166II GTNNAC 5 cut(s) 13, 211, 1019, 1813, 2264
Hpy8I GTNNAC 5 cut(s) 13, 211, 1019, 1813, 2264
Hpy99I CGWCG 6 cut(s) 17, 20, 59, 107, 212, 1644
HpyAV CCTTC 4 cut(s) 289, 902, 964, 2044
HpyCH4III ACNGT 9 cut(s) 94, 479, 733, 1172, 1805, 1879, 2066, 2654, 2767
HpyCH4IV ACGT 2 cut(s) 176, 402
HpyF10VI GCNNNNNNNGC 2 cut(s) 1277, 1286
HpyF3I CTNAG 5 cut(s) 150, 285, 1411, 2396, 2412
HpySE526I ACGT 2 cut(s) 176, 402
Hsp92I GRCGYC 2 cut(s) 18, 245
Hsp92II CATG 8 cut(s) 1337, 1403, 1594, 1838, 2386, 2483, 2631, 2791
HspAI GCGC 3 cut(s) 245, 1838, 2463
KasI GGCGCC 1 cut(s) 244
Kpn2I TCCGGA 1 cut(s) 2980
Ksp22I TGATCA 1 cut(s) 1951
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 4 cut(s) 329, 604, 1247, 2885
Lsp1109I GCAGC 5 cut(s) 946, 1162, 1264, 1312, 2758
MabI ACCWGGT 1 cut(s) 2438
MaeI CTAG 4 cut(s) 1389, 1679, 2780, 2973
MaeII ACGT 2 cut(s) 176, 402
MaeIII GTNAC 6 cut(s) 46, 121, 186, 1120, 1618, 2313
MflI RGATCY 5 cut(s) 1339, 1783, 1864, 2275, 2348
MhlI GDGCHC 5 cut(s) 162, 291, 1021, 1309, 2687
MlsI TGGCCA 1 cut(s) 2847
MluNI TGGCCA 1 cut(s) 2847
Mly113I GGCGCC 1 cut(s) 245
MlyI GAGTC 5 cut(s) 145, 301, 365, 395, 440
MmeI TCCRAC 4 cut(s) 492, 540, 876, 1242
Mox20I TGGCCA 1 cut(s) 2847
Mph1103I ATGCAT 4 cut(s) 766, 1234, 1513, 2815
MroI TCCGGA 1 cut(s) 2980
MscI TGGCCA 1 cut(s) 2847
MseI TTAA 8 cut(s) 1203, 1503, 1932, 2387, 2751, 2876, 2901, 2963
MslI CAYNNNNRTG 2 cut(s) 1425, 2808
Msp20I TGGCCA 1 cut(s) 2847
MspI CCGG 3 cut(s) 21, 375, 2981
MspR9I CCNGG 6 cut(s) 22, 531, 1032, 1040, 2440, 2895
MvaI CCWGG 5 cut(s) 531, 1032, 1040, 2440, 2895
MvnI CGCG 2 cut(s) 293, 2375
MwoI GCNNNNNNNGC 2 cut(s) 1277, 1286
NarI GGCGCC 1 cut(s) 245
NciI CCSGG 1 cut(s) 22
NdeI CATATG 1 cut(s) 1585
NlaIII CATG 8 cut(s) 1337, 1403, 1594, 1838, 2386, 2483, 2631, 2791
NlaIV GGNNCC 7 cut(s) 246, 538, 792, 1341, 1866, 2277, 2887
NmuCI GTSAC 3 cut(s) 46, 186, 2313
NsiI ATGCAT 4 cut(s) 766, 1234, 1513, 2815
NspV TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
PciSI GCTCTTC 1 cut(s) 351
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 111, 131, 408
PflMI CCANNNNNTGG 2 cut(s) 1205, 1475
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 7 cut(s) 324, 961, 1177, 1279, 1327, 2377, 2773
PleI GAGTC 5 cut(s) 144, 301, 365, 394, 439
PluTI GGCGCC 1 cut(s) 248
PpsI GAGTC 5 cut(s) 144, 301, 365, 394, 439
Ppu21I YACGTR 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 536
Psp6I CCWGG 5 cut(s) 529, 1030, 1038, 2438, 2893
PspGI CCWGG 5 cut(s) 529, 1030, 1038, 2438, 2893
PspN4I GGNNCC 7 cut(s) 246, 538, 792, 1341, 1866, 2277, 2887
PspPI GGNCC 4 cut(s) 527, 536, 722, 1813
PspPPI RGGWCCY 1 cut(s) 536
PsrI GAACNNNNNNTAC 2 cut(s) 2580, 2612
PstI CTGCAG 1 cut(s) 2884
PstNI CAGNNNCTG 1 cut(s) 1244
PsuI RGATCY 5 cut(s) 1339, 1783, 1864, 2275, 2348
RsaI GTAC 3 cut(s) 179, 476, 2174
RsaNI GTAC 3 cut(s) 178, 475, 2173
RseI CAYNNNNRTG 2 cut(s) 1425, 2808
SalI GTCGAC 2 cut(s) 11, 209
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 8 cut(s) 1203, 1503, 1932, 2387, 2751, 2876, 2901, 2963
SatI GCNGC 7 cut(s) 323, 960, 1176, 1278, 1326, 2376, 2772
Sau96I GGNCC 4 cut(s) 527, 536, 722, 1813
SchI GAGTC 5 cut(s) 145, 301, 365, 395, 440
ScrFI CCNGG 6 cut(s) 22, 531, 1032, 1040, 2440, 2895
SduI GDGCHC 5 cut(s) 162, 291, 1021, 1309, 2687
SexAI ACCWGGT 1 cut(s) 2438
SfcI CTRYAG 2 cut(s) 2270, 2880
SfoI GGCGCC 1 cut(s) 246
SfuI TTCGAA 6 cut(s) 502, 518, 647, 683, 833, 869
SinI GGWCC 4 cut(s) 527, 536, 722, 1813
SmiMI CAYNNNNRTG 2 cut(s) 1425, 2808
SmlI CTYRAG 3 cut(s) 2544, 2723, 2995
SmoI CTYRAG 3 cut(s) 2544, 2723, 2995
SspDI GGCGCC 1 cut(s) 244
SspI AATATT 1 cut(s) 1534
SspMI CTAG 4 cut(s) 1389, 1679, 2780, 2973
StyD4I CCNGG 6 cut(s) 20, 529, 1030, 1038, 2438, 2893
StyI CCWWGG 2 cut(s) 390, 2569
TaaI ACNGT 9 cut(s) 94, 479, 733, 1172, 1805, 1879, 2066, 2654, 2767
TaiI ACGT 2 cut(s) 179, 405
TaqII GACCGA 1 cut(s) 515
TatI WGTACW 1 cut(s) 2172
TauI GCSGC 2 cut(s) 325, 2378
Tru1I TTAA 8 cut(s) 1203, 1503, 1932, 2387, 2751, 2876, 2901, 2963
Tru9I TTAA 8 cut(s) 1203, 1503, 1932, 2387, 2751, 2876, 2901, 2963
TscAI CASTG 5 cut(s) 574, 760, 1177, 1432, 1732
TseFI GTSAC 3 cut(s) 46, 186, 2313
TseI GCWGC 5 cut(s) 959, 1175, 1277, 1325, 2771
Tsp45I GTSAC 3 cut(s) 46, 186, 2313
TspGWI ACGGA 6 cut(s) 93, 140, 272, 627, 756, 1796
TspRI CASTG 5 cut(s) 574, 760, 1177, 1432, 1732
Van91I CCANNNNNTGG 2 cut(s) 1205, 1475
VneI GTGCAC 1 cut(s) 1017
VpaK11BI GGWCC 4 cut(s) 527, 536, 722, 1813
XmiI GTMKAC 2 cut(s) 12, 210
XspI CTAG 4 cut(s) 1389, 1679, 2780, 2973
Zsp2I ATGCAT 4 cut(s) 766, 1234, 1513, 2815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.