RLG00000025433

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004748
Physical Location & Seq
Reverse (-)
45748260 .. 45753412
5153 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025433

Sequence Viewer

Length: 2913 bp
ATGGCGGACGACGACGACGGCTTCGGAGACTTCACTTTCGTCTCCGCCGCCGCCGCCTTCAATCCCAATCCCACCACCACCCAACGACAATCGGCCGCCTCCGACGACGATTGGGGCGATTTCGTAACCGTCGCCCCGCCTCCGATCAAAACCCCCGTCGTTAACGGATTCGCACCCGCTCCGGCCCAAACACCGCCCGACCCGTTCGGGTTTTTCGACGTTTCTAACGGCGCCGCAGCAGCGCGGTTAACGTCAGCCGGGTCGGATCCAATCCGGGTCGAGACCACGGCCGAAGGGATTAGTAAGATGACTCAGTGGGAGAAGCCGACGGGAGCTTTGCCGTTGTCGTTGTTTGGGGAAGAGGAGAGAGACGAGCAGGTGAATCAGGATTTGACTAAGAATGAGGGATTTGGGAAAAAGGGATCGAATTTGAATGGGAGTAGCGGTGGGATTGATGATTTGATATCGAATTTGTATGTTCAGAACGGGTCGAATCGGAATTCTGGTTCGGGTGGTATTGATTTTAGCAAGAGCGGGTTGAATTTGAGGTCGAATGGGGTGGATTCGAAGCTTGGTGGTTTGATTTCGAGCCAAAATGGGAAGCTGGATGGTGTGGATTTGAGTTCGAATGGTTTGAAATTTTTTGAGGGTGTAGATTTGAATGGGAGCTCAAATTCAAGTGTGTTGAAGATGAATGTGGAAGAAGGGAATCGGGATTTTGACGAGGATGATGATGATGGTTGGGAATTCAAGGCTGCAGAACCAGAGAACTGGGTTGGCAATGAGAATTCTAAGGAACGAGGAATTTTGGAAGTAGCAGAGTCAAAAGCAGAATCAAGTACACTTGTAAACGGGGGAATACAGGAAAATACCGGAGGAACTGGATTTTCTGGGGGGTTTGGTATTCATGAGTTTAATAATGTGTCTGTTGCATCTAATGGAACTTCTCGTGGAAATGGTGAATGGGGTTTTTCATTTGACTTCAAGTCAAGCCCTGTATCTCAAGATAATTTCTTCTCGGAAGCATACTCGAAAAGCAAGCAGAGCAATGCTGCAACTGTATCCAATTCTTCCGGAGTTGATGATAATGTGTGGGGATTCAAGGATGCACTTTCGGGAACTGGATCAGAACATAAGTCGGAAGATGCTAAGGCTGCTACTCCTGCTGGTTTAGAATTGCATACATTAGATGGTGCTGGTGCTGGTCCTCGTAATGATTTTTTTGCCGGGTCAGATTGGATTTCTCACCAGTCTTCTGAAAGTAACTTTGCATTTCCTTTCATCCCAAATTCTGGCAATAAAGATGGCATAATCTCAGATTCCTATTCCAGTGGCAAGAAAGATGACATCGTAACAGGATTGAGTTTTCCTCCAGATAATGACCATGTTGAGTCAGAAGACAATTTTTGGGAATTTAAGGATGCATTTTCAGAATCTGGATCTAAGTTTGAGGGGGAGTTGGTGGTTGCCGGTAATTCTCCTACCAATATAAAACCATCAGCAATGGCTGGAGAAAATCAGCACAGTGAGGTAACATTGAATAATCATCGGCAAGCTTTACCCTTGTCCATTTTTGGAGACGGAGAACTAGAAACTGATGATTCCTCAGTCCATCAAGATATTTCTACTCAGACAGCTGCTTCCCACCAGATAAATACTGCCAAAAGCCCTGCTACCAATGTATCCATCACTGATCTCATATCAAGCTTATACAGTCAAGTGGAACAGAACACTTCTAAACTTCATACGCCAAAAGAAACTGAAAGTACTATGCATCCTGTGACGACAGTGTTAGACTCTGATTTTGTTGGTGATGATGCTGATGATGACTCCTGGGAGTTTAAGGATGCTGTTTCAAAAGATCGAGACCCAACTTCTATTGCCAATCTTGAAGATTCACTTAAACATTCTTGTACTAAAATCCAGCTGGATGACTTTGTGGAATTGTATTGCAAGTTGAAGAATGAATCATATTTTCTTGCACTCTACCATCTTGACCAGAAAAAGAAAGCTCAAAGTAGTGCTTCTCTTTCTGGTGACGATGCAACAGACGCTGTGGATAAGGAAATCCAGAAACTTTATAATGAACCGCCTCAAGACAACATGAGATTAGATCAGTTCCAGTCAGGGAATCCTTCACCAAGAAATGAATGCCTTAACGAACTTCATAAAGCTCTGCAGGAACTGAAGTTCCAAGTCCTTGAGTCAGAATATCAATTGTCACAAAGATTGTCCTTGGCAGAGAAGGATTTGATATCAGCAATTGAACTTCTAAGACATGCAGCAGCCATATTAAGGATTCTTAGACTCGGATCAGCTGAGGAGCAATCTAGGTACATTGCTGCATGGTCTCAAATGGTCTCCATATGTTCTGAAGAGCTGAAACATGGCTCTTTGATTTGGATGCAATCATTAGAGAAGAATGTTCAGAAGCAGATGTTATCTGAACCTCAAGGCAAGCAGTATATCGTTGCTCTGGGAGAAATTTACCGGGTAGTTTTAGTTCTTGGAGCCTCAATTAAACTATACAGCCCTTGGATTCTATTACAGTCCTCAGATTGTTCTAGCTTGTTTGGTCTTCTAAACGAGTGTTCTACTCTATGGTCAAGTTCAGGACTTGATGAAGCTCTCAGAAGTATTTCAGATGTTGATGATTTCAAATATGACGGAAATGTTAATGCATTATTGGATTCCTTAACGTCTATTAATCATCTCGATGCATCTTCACTCCAAAACCATTTTTTGAGTGGACAACAAGCTATATGTTCACTGTCACTTTTAACTGCAGAAGCAGTGCCAGGTATTAAAATGGTGGCGTGGAATGAGGTATACTATTTATTGACACTCGCAAACTTGTGGATAAATTTAATTGGTGGTGAACCCCCACGGTTGCCGCACTTCAGTTTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

971

Amino Acids

104.87

Weight (kDa)

4.47

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYNRG_C PF25999 773 - 964 1.8e-60 Synergin gamma, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2082
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 1292
AccBSI CCGCTC 2 cut(s) 179, 534
AccI GTMKAC 1 cut(s) 2829
AccII CGCG 1 cut(s) 244
AccIII TCCGGA 1 cut(s) 1073
AclWI GGATC 6 cut(s) 260, 273, 430, 1132, 1447, 2320
AcoI YGGCCR 2 cut(s) 93, 288
AcuI CTGAAG 3 cut(s) 2207, 2394, 2884
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 4 cut(s) 841, 1768, 1915, 2336
AfiI CCNNNNNNNGG 2 cut(s) 1292, 2295
AhdI GACNNNNNGTC 1 cut(s) 985
AjnI CCWGG 2 cut(s) 1832, 2797
AloI GAACNNNNNNTCC 6 cut(s) 490, 522, 2174, 2175, 2206, 2207
Alw21I GWGCWC 1 cut(s) 671
Alw26I GTCTC 8 cut(s) 21, 46, 275, 363, 1572, 1860, 2355, 2365
AlwI GGATC 6 cut(s) 260, 273, 430, 1132, 1447, 2320
AlwNI CAGNNNCTG 3 cut(s) 1436, 2054, 2185
Aor13HI TCCGGA 1 cut(s) 1073
AoxI GGCC 3 cut(s) 93, 183, 288
ApeKI GCWGC 9 cut(s) 236, 239, 755, 1052, 1154, 1637, 2282, 2285, 2342
ArsI GACNNNNNNTTYG 9 cut(s) 20, 37, 52, 141, 173, 319, 351, 1787, 1819
AseI ATTAAT 1 cut(s) 2706
Asp700I GAANNNNTTC 1 cut(s) 2638
AspLEI GCGC 2 cut(s) 233, 244
AspS9I GGNCC 2 cut(s) 184, 1205
AsuC2I CCSGG 4 cut(s) 259, 275, 1228, 2492
AsuHPI GGTGA 7 cut(s) 391, 971, 1238, 1823, 2048, 2130, 2888
AsuII TTCGAA 2 cut(s) 566, 626
AvaII GGWCC 1 cut(s) 1205
BaeI ACNNNNGTAYC 2 cut(s) 1665, 1698
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 671
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 3 cut(s) 1245, 1404, 2570
Bbv12I GWGCWC 1 cut(s) 671
BbvCI CCTCAGC 1 cut(s) 2319
BbvI GCAGC 9 cut(s) 248, 251, 742, 1039, 1141, 1624, 2294, 2297, 2329
BccI CCATC 8 cut(s) 602, 731, 1184, 1298, 1504, 1620, 1694, 1998
BceAI ACGGC 4 cut(s) 34, 244, 303, 325
BciT130I CCWGG 2 cut(s) 1834, 2799
BciVI GTATCC 2 cut(s) 1072, 1693
BcnI CCSGG 4 cut(s) 259, 275, 1228, 2492
BcoDI GTCTC 8 cut(s) 21, 46, 275, 363, 1572, 1860, 2355, 2365
BfaI CTAG 3 cut(s) 1589, 2331, 2565
BfmI CTRYAG 3 cut(s) 756, 2177, 2784
BfoI RGCGCY 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 367
BfuI GTATCC 2 cut(s) 1072, 1693
BmcAI AGTACT 1 cut(s) 1768
Bme1390I CCNGG 6 cut(s) 259, 275, 1228, 1834, 2492, 2799
Bme18I GGWCC 1 cut(s) 1205
BmeRI GACNNNNNGTC 1 cut(s) 985
BmgT120I GGNCC 2 cut(s) 184, 1205
BmiI GGNNCC 3 cut(s) 232, 267, 2512
BmrFI CCNGG 6 cut(s) 259, 275, 1228, 1834, 2492, 2799
BmrI ACTGGG 1 cut(s) 781
BmuI ACTGGG 1 cut(s) 781
BpiI GAAGAC 3 cut(s) 1245, 1404, 2570
BplI GAGNNNNNCTC 2 cut(s) 1354, 1386
BpmI CTGGAG 2 cut(s) 1356, 1530
Bpu10I CCTNAGC 2 cut(s) 1149, 2319
Bpu14I TTCGAA 2 cut(s) 566, 626
BpuEI CTTGAG 4 cut(s) 987, 2079, 2222, 2436
BpuMI CCSGG 4 cut(s) 259, 275, 1228, 2492
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 4 cut(s) 275, 1860, 2355, 2365
BsaJI CCNNGG 5 cut(s) 285, 1833, 2235, 2534, 2885
BsaWI WCCGGW 2 cut(s) 872, 1073
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 1292, 2295
Bse118I RCCGGY 1 cut(s) 1469
Bse1I ACTGG 6 cut(s) 776, 886, 1126, 1249, 1329, 2122
Bse3DI GCAATG 4 cut(s) 787, 1054, 1509, 2337
BseAI TCCGGA 1 cut(s) 1073
BseBI CCWGG 2 cut(s) 1834, 2799
BseDI CCNNGG 5 cut(s) 285, 1833, 2235, 2534, 2885
BseGI GGATG 9 cut(s) 613, 733, 1111, 1281, 1426, 1774, 1852, 1936, 2409
BseLI CCNNNNNNNGG 2 cut(s) 1292, 2295
BseMI GCAATG 4 cut(s) 787, 1054, 1509, 2337
BseMII CTCAG 7 cut(s) 326, 1329, 1620, 1643, 2310, 2568, 2644
BseNI ACTGG 6 cut(s) 776, 886, 1126, 1249, 1329, 2122
BseRI GAGGAG 2 cut(s) 377, 2336
BseX3I CGGCCG 2 cut(s) 93, 288
BseXI GCAGC 9 cut(s) 248, 251, 742, 1039, 1141, 1624, 2294, 2297, 2329
Bsh1236I CGCG 1 cut(s) 244
Bsh1285I CGRYCG 2 cut(s) 96, 291
BshFI GGCC 3 cut(s) 95, 185, 290
BshNI GGYRCC 1 cut(s) 230
BsiEI CGRYCG 2 cut(s) 96, 291
BsiHKAI GWGCWC 1 cut(s) 671
BsiSI CCGG 8 cut(s) 182, 258, 274, 873, 1074, 1227, 1470, 2491
BslI CCNNNNNNNGG 2 cut(s) 1292, 2295
BsmAI GTCTC 8 cut(s) 21, 46, 275, 363, 1572, 1860, 2355, 2365
BsmBI CGTCTC 3 cut(s) 46, 363, 1572
BsmI GAATGC 1 cut(s) 2156
BsnI GGCC 3 cut(s) 95, 185, 290
Bso31I GGTCTC 4 cut(s) 275, 1860, 2355, 2365
Bsp119I TTCGAA 2 cut(s) 566, 626
Bsp1286I GDGCHC 1 cut(s) 671
Bsp13I TCCGGA 1 cut(s) 1073
Bsp143I GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
BspANI GGCC 3 cut(s) 95, 185, 290
BspCNI CTCAG 7 cut(s) 325, 1328, 1619, 1642, 2311, 2567, 2643
BspEI TCCGGA 1 cut(s) 1073
BspFNI CGCG 1 cut(s) 244
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 3 cut(s) 232, 267, 2512
BspMAI CTGCAG 3 cut(s) 760, 2181, 2788
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 6 cut(s) 260, 273, 430, 1132, 1447, 2320
BspQI GCTCTTC 1 cut(s) 2370
BspT104I TTCGAA 2 cut(s) 566, 626
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 4 cut(s) 275, 1860, 2355, 2365
BsrBI CCGCTC 2 cut(s) 179, 534
BsrDI GCAATG 4 cut(s) 787, 1054, 1509, 2337
BsrFI RCCGGY 1 cut(s) 1469
BsrI ACTGG 6 cut(s) 776, 886, 1126, 1249, 1329, 2122
BssAI RCCGGY 1 cut(s) 1469
BssECI CCNNGG 5 cut(s) 285, 1833, 2235, 2534, 2885
BssMI GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
BssNAI GTATAC 1 cut(s) 2830
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 2235, 2534
Bst1107I GTATAC 1 cut(s) 2830
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 2 cut(s) 1834, 2799
Bst4CI ACNGT 8 cut(s) 130, 1060, 1526, 1715, 1789, 2550, 2772, 2889
Bst6I CTCTTC 2 cut(s) 354, 2370
BstACI GRCGYC 1 cut(s) 231
BstBI TTCGAA 2 cut(s) 566, 626
BstC8I GCNNGC 3 cut(s) 1040, 1554, 2459
BstDSI CCRYGG 2 cut(s) 285, 2885
BstF5I GGATG 9 cut(s) 613, 733, 1111, 1281, 1426, 1774, 1852, 1936, 2409
BstFNI CGCG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 2 cut(s) 233, 244
BstKTI GATC 9 cut(s) 147, 268, 425, 1127, 1442, 1696, 1864, 2116, 2315
BstMAI GTCTC 8 cut(s) 21, 46, 275, 363, 1572, 1860, 2355, 2365
BstMBI GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
BstMCI CGRYCG 2 cut(s) 96, 291
BstMWI GCNNNNNNNGC 6 cut(s) 53, 239, 1044, 1154, 1163, 2051
BstNI CCWGG 2 cut(s) 1834, 2799
BstNSI RCATGY 1 cut(s) 2282
BstSCI CCNGG 6 cut(s) 257, 273, 1226, 1832, 2490, 2797
BstSFI CTRYAG 3 cut(s) 756, 2177, 2784
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 9 cut(s) 248, 251, 742, 1039, 1141, 1624, 2294, 2297, 2329
BstV2I GAAGAC 3 cut(s) 1245, 1404, 2570
BstX2I RGATCY 2 cut(s) 265, 1439
BstXI CCANNNNNNTGG 1 cut(s) 771
BstYI RGATCY 2 cut(s) 265, 1439
BstZ17I GTATAC 1 cut(s) 2830
BstZI CGGCCG 2 cut(s) 93, 288
BsuI GTATCC 2 cut(s) 1072, 1693
BsuRI GGCC 3 cut(s) 95, 185, 290
BtgI CCRYGG 2 cut(s) 285, 2885
BtsCI GGATG 9 cut(s) 613, 733, 1111, 1281, 1426, 1774, 1852, 1936, 2409
BtsI GCAGTG 1 cut(s) 2799
BtsIMutI CAGTG 7 cut(s) 320, 1336, 1531, 1689, 1794, 2768, 2799
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 3 cut(s) 1040, 1554, 2459
CaiI CAGNNNCTG 3 cut(s) 1436, 2054, 2185
CciI TCATGA 1 cut(s) 907
CfoI GCGC 2 cut(s) 233, 244
Cfr10I RCCGGY 1 cut(s) 1469
Cfr13I GGNCC 2 cut(s) 184, 1205
CseI GACGC 1 cut(s) 2060
Csp6I GTAC 4 cut(s) 840, 1767, 1914, 2335
CviAII CATG 6 cut(s) 908, 1385, 2104, 2279, 2346, 2387
CviQI GTAC 4 cut(s) 840, 1767, 1914, 2335
DinI GGCGCC 1 cut(s) 232
DpnI GATC 9 cut(s) 146, 267, 424, 1126, 1441, 1695, 1863, 2115, 2314
DpnII GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
DriI GACNNNNNGTC 1 cut(s) 985
EaeI YGGCCR 2 cut(s) 93, 288
EagI CGGCCG 2 cut(s) 93, 288
Eam1104I CTCTTC 2 cut(s) 354, 2370
Eam1105I GACNNNNNGTC 1 cut(s) 985
EarI CTCTTC 2 cut(s) 354, 2370
EciI GGCGGA 2 cut(s) 20, 34
Ecl136II GAGCTC 1 cut(s) 669
EclXI CGGCCG 2 cut(s) 93, 288
Eco130I CCWWGG 2 cut(s) 2235, 2534
Eco24I GRGCYC 1 cut(s) 671
Eco31I GGTCTC 4 cut(s) 275, 1860, 2355, 2365
Eco32I GATATC 2 cut(s) 465, 2256
Eco47I GGWCC 1 cut(s) 1205
Eco52I CGGCCG 2 cut(s) 93, 288
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 3 cut(s) 2207, 2394, 2884
EcoICRI GAGCTC 1 cut(s) 669
EcoRI GAATTC 3 cut(s) 499, 746, 787
EcoRII CCWGG 2 cut(s) 1832, 2797
EcoRV GATATC 2 cut(s) 465, 2256
EcoT14I CCWWGG 2 cut(s) 2235, 2534
EcoT22I ATGCAT 4 cut(s) 1426, 1776, 2683, 2722
EcoT38I GRGCYC 1 cut(s) 671
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 2235, 2534
Esp3I CGTCTC 3 cut(s) 46, 363, 1572
FaeI CATG 6 cut(s) 911, 1388, 2107, 2282, 2349, 2390
FalI AAGNNNNNCTT 4 cut(s) 1884, 1916, 2008, 2040
FatI CATG 6 cut(s) 907, 1384, 2103, 2278, 2345, 2386
FauI CCCGC 3 cut(s) 144, 184, 527
FauNDI CATATG 1 cut(s) 2366
FblI GTMKAC 1 cut(s) 2829
FokI GGATG 9 cut(s) 620, 740, 1118, 1268, 1433, 1761, 1859, 1943, 2416
FriOI GRGCYC 1 cut(s) 671
FspBI CTAG 3 cut(s) 1589, 2331, 2565
GlaI GCGC 2 cut(s) 232, 243
GsuI CTGGAG 2 cut(s) 1356, 1530
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 3 cut(s) 95, 185, 290
HapII CCGG 8 cut(s) 182, 258, 274, 873, 1074, 1227, 1470, 2491
HgaI GACGC 1 cut(s) 2060
HhaI GCGC 2 cut(s) 233, 244
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 6 cut(s) 911, 1388, 2107, 2282, 2349, 2390
Hin6I GCGC 2 cut(s) 231, 242
HinP1I GCGC 2 cut(s) 231, 242
HincII GTYRAC 2 cut(s) 163, 249
HindII GTYRAC 2 cut(s) 163, 249
HindIII AAGCTT 3 cut(s) 569, 1554, 1705
HpaI GTTAAC 2 cut(s) 163, 249
HpaII CCGG 8 cut(s) 182, 258, 274, 873, 1074, 1227, 1470, 2491
HphI GGTGA 7 cut(s) 391, 971, 1238, 1823, 2048, 2130, 2888
Hpy166II GTNNAC 8 cut(s) 163, 249, 842, 850, 2750, 2768, 2830, 2879
Hpy8I GTNNAC 8 cut(s) 163, 249, 842, 850, 2750, 2768, 2830, 2879
Hpy99I CGWCG 9 cut(s) 14, 17, 20, 107, 110, 134, 161, 221, 331
HpyAV CCTTC 5 cut(s) 67, 287, 698, 2145, 2239
HpyCH4III ACNGT 8 cut(s) 130, 1060, 1526, 1715, 1789, 2550, 2772, 2889
HpyCH4IV ACGT 3 cut(s) 219, 251, 2699
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 239, 1044, 1154, 1163, 2051
HpySE526I ACGT 3 cut(s) 219, 251, 2699
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 6 cut(s) 911, 1388, 2107, 2282, 2349, 2390
HspAI GCGC 2 cut(s) 231, 242
KasI GGCGCC 1 cut(s) 230
Kpn2I TCCGGA 1 cut(s) 1073
KspAI GTTAAC 2 cut(s) 163, 249
Kzo9I GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
LguI GCTCTTC 1 cut(s) 2370
LmnI GCTCC 5 cut(s) 184, 332, 666, 2323, 2510
Lsp1109I GCAGC 9 cut(s) 248, 251, 742, 1039, 1141, 1624, 2294, 2297, 2329
MaeI CTAG 3 cut(s) 1589, 2331, 2565
MaeII ACGT 3 cut(s) 219, 251, 2699
MaeIII GTNAC 8 cut(s) 124, 1262, 1351, 1531, 1780, 2036, 2220, 2772
MalI GATC 9 cut(s) 146, 267, 424, 1126, 1441, 1695, 1863, 2115, 2314
MbiI CCGCTC 2 cut(s) 179, 534
MboI GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
MfeI CAATTG 2 cut(s) 2216, 2262
MflI RGATCY 2 cut(s) 265, 1439
MhlI GDGCHC 1 cut(s) 671
Mly113I GGCGCC 1 cut(s) 231
MlyI GAGTC 7 cut(s) 304, 830, 1400, 1790, 1823, 2213, 2301
MmeI TCCRAC 3 cut(s) 126, 243, 1119
Mph1103I ATGCAT 4 cut(s) 1426, 1776, 2683, 2722
MroI TCCGGA 1 cut(s) 1073
MroXI GAANNNNTTC 1 cut(s) 2638
MslI CAYNNNNRTG 1 cut(s) 2715
MspA1I CMGCKG 3 cut(s) 1637, 1927, 2318
MspI CCGG 8 cut(s) 182, 258, 274, 873, 1074, 1227, 1470, 2491
MspR9I CCNGG 6 cut(s) 259, 275, 1228, 1834, 2492, 2799
MunI CAATTG 2 cut(s) 2216, 2262
Mva1269I GAATGC 1 cut(s) 2156
MvaI CCWGG 2 cut(s) 1834, 2799
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 6 cut(s) 53, 239, 1044, 1154, 1163, 2051
NarI GGCGCC 1 cut(s) 231
NciI CCSGG 4 cut(s) 259, 275, 1228, 2492
NdeI CATATG 1 cut(s) 2366
NdeII GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
NlaIII CATG 6 cut(s) 911, 1388, 2107, 2282, 2349, 2390
NlaIV GGNNCC 3 cut(s) 232, 267, 2512
NmuCI GTSAC 4 cut(s) 1780, 2036, 2220, 2772
NsiI ATGCAT 4 cut(s) 1426, 1776, 2683, 2722
NspI RCATGY 1 cut(s) 2282
NspV TTCGAA 2 cut(s) 566, 626
PagI TCATGA 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 2370
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 213
PctI GAATGC 1 cut(s) 2156
PdmI GAANNNNTTC 1 cut(s) 2638
PflMI CCANNNNNTGG 1 cut(s) 1292
PleI GAGTC 7 cut(s) 304, 829, 1399, 1790, 1823, 2212, 2301
PluTI GGCGCC 1 cut(s) 234
PpsI GAGTC 7 cut(s) 304, 829, 1399, 1790, 1823, 2212, 2301
PshBI ATTAAT 1 cut(s) 2706
PsiI TTATAA 1 cut(s) 2082
Psp124BI GAGCTC 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 1832, 2797
PspGI CCWGG 2 cut(s) 1832, 2797
PspN4I GGNNCC 3 cut(s) 232, 267, 2512
PspPI GGNCC 2 cut(s) 184, 1205
PsrI GAACNNNNNNTAC 2 cut(s) 2487, 2519
PstI CTGCAG 3 cut(s) 760, 2181, 2788
PstNI CAGNNNCTG 3 cut(s) 1436, 2054, 2185
PsuI RGATCY 2 cut(s) 265, 1439
PvuII CAGCTG 3 cut(s) 1637, 1927, 2318
RsaI GTAC 4 cut(s) 841, 1768, 1915, 2336
RsaNI GTAC 4 cut(s) 840, 1767, 1914, 2335
RseI CAYNNNNRTG 1 cut(s) 2715
SacI GAGCTC 1 cut(s) 671
SapI GCTCTTC 1 cut(s) 2370
Sau3AI GATC 9 cut(s) 144, 265, 422, 1124, 1439, 1693, 1861, 2113, 2312
Sau96I GGNCC 2 cut(s) 184, 1205
ScaI AGTACT 1 cut(s) 1768
SchI GAGTC 7 cut(s) 304, 830, 1400, 1790, 1823, 2213, 2301
ScrFI CCNGG 6 cut(s) 259, 275, 1228, 1834, 2492, 2799
SduI GDGCHC 1 cut(s) 671
SfcI CTRYAG 3 cut(s) 756, 2177, 2784
SfoI GGCGCC 1 cut(s) 232
SfuI TTCGAA 2 cut(s) 566, 626
SinI GGWCC 1 cut(s) 1205
SmiMI CAYNNNNRTG 1 cut(s) 2715
SmlI CTYRAG 4 cut(s) 1002, 2094, 2201, 2451
SmoI CTYRAG 4 cut(s) 1002, 2094, 2201, 2451
SspDI GGCGCC 1 cut(s) 230
SspMI CTAG 3 cut(s) 1589, 2331, 2565
SstI GAGCTC 1 cut(s) 671
StyD4I CCNGG 6 cut(s) 257, 273, 1226, 1832, 2490, 2797
StyI CCWWGG 2 cut(s) 2235, 2534
TaaI ACNGT 8 cut(s) 130, 1060, 1526, 1715, 1789, 2550, 2772, 2889
TaiI ACGT 3 cut(s) 222, 254, 2702
TatI WGTACW 3 cut(s) 839, 1766, 1913
TauI GCSGC 6 cut(s) 50, 53, 56, 98, 236, 2896
TscAI CASTG 7 cut(s) 320, 1336, 1531, 1696, 1794, 2775, 2799
TseFI GTSAC 4 cut(s) 1780, 2036, 2220, 2772
TseI GCWGC 9 cut(s) 236, 239, 755, 1052, 1154, 1637, 2282, 2285, 2342
Tsp45I GTSAC 4 cut(s) 1780, 2036, 2220, 2772
TspGWI ACGGA 3 cut(s) 180, 1596, 2682
TspRI CASTG 7 cut(s) 320, 1336, 1531, 1696, 1794, 2775, 2799
Van91I CCANNNNNTGG 1 cut(s) 1292
VpaK11BI GGWCC 1 cut(s) 1205
VspI ATTAAT 1 cut(s) 2706
XceI RCATGY 1 cut(s) 2282
XcmI CCANNNNNNNNNTGG 1 cut(s) 2744
XmiI GTMKAC 1 cut(s) 2829
XmnI GAANNNNTTC 1 cut(s) 2638
XspI CTAG 3 cut(s) 1589, 2331, 2565
ZrmI AGTACT 1 cut(s) 1768
Zsp2I ATGCAT 4 cut(s) 1426, 1776, 2683, 2722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.