Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001548

Physical Location & Seq

Genomic Coordinates Reverse (-)

29908762 .. 29913697

Gene Structure 4936 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1216500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGGCTGTGTGATTAGTAGAGAAGCTTCTTCGGGAGTCAATTCTGAGTCGAAGGGGGAGAGGAGGGATTTGAGTGTAGAGTCTACTGGGAAGGTAGATGCTGTTGTGGTGCCGAAGGTAGATAGTAAAACCGCCGAGGTTCATGTTGGTGGGGGAGAGGGCAAGAAGGAGGAGAATGTCCATGGAGGTGAGAAACCTCCGAGGGGGGAGAGAAGGCGATCGAAGCCAAACCCGAGGTCAGGTAATGTACCAAAGCAATCGCGTGGCGCCCAAGTGGCGGCCGGCTGGCCACCCTGGCTCACTGAGGCATGTGGGGAGGCGCTCAATGGGTGGATTCCACGGAAAGCAGACACGTTTGAGAAAATTGATAAGATTGGGTCAGGAACGTATAGCAATGTGTACAAAGCTAAAGATATGTTGACGGGGAAAATTGTTGCCCTAAAGAAAGTTAGATTTGACAATTTGGAGCCCGAGAGCGTGAGATTTATGGCTAGAGAAATTCTAATTTTGCGGAGATTGGATCATATCAATGTTGTAAAGTTGGAGGGTCTGGTTACGTCAAGAATGTCATGTAGTTTGTACCTGGTGTTGGAATACATGGAGCATGATCTAGCTGGGCTTGCTGCAAGCCCCGAAATTAAATTTACAGAACCACAGGTAAAATGTTACATGCATCAACTTCTATCTGGACTTGAGCACTGTCACAACCGTGGTGTGCTTCACCGTGACATTAAAGGATCAAATCTTTTGATTGACAATGGAGGAGTACTTAAAATTGCTGACTTTGGATTGGCTTCTTTTTTTGATCCAAACCATAAGCATCCAATGACTAGTCGGGTGGTTACTCTATGGTATCGACCTCCCGAGCTCCTTCTTGGGGCTACTGATTACGGTGTGGGTGTGGACCTCTGGAGTGCAGGTTGCATTTTAGCGGAGTTACTAGCTGGGAAGCCTATCATGCCTGGTCGTACCGAGGTGGAGCAACTACATAAGATATACAAACTATGTGGTTCACCTTCAGATGAATACTGGAGGAAAGCAAAGTTGCCCAATGCAACGCTATTTAGGCCTCGGGAGCCTTACAGAAGATACATAAGAGAAGCATTCAAAGATTTTCCCCCATCTTCATTTCCCCTTATCGAAACTCTTCTTGCAATTGATCCAGCAGAACGTCAGACAGCCACAGCTGCATTGAGTAGTGTGTTTTTCCTGTCAGAGCCTTATGCTTGTGAACCTTCTAGCCTCCCAAAGTATCCCCCAACCAAAGAAATAGACGCCAAACGCCGGGACGATGAAGCTCGTAGACTACGAGCTGCTGGTAGATCCCAGGCTGATGGTCCAAAGAAAATACGCACACGGGAACGGGCTCCTAGGGGTGGTATGCCGGCTCCAGAAGCCAATGCGGAGCTTCAGTCCAATATTGATAGAAGGCGCCTAATTACGCATGCAAATGCAAAGAGCAAAAGCGAAAAGTTTCCTCCGCCACATCAGGATGGAGCAGTTGGCTACCCTTTGGGATCTTCGCATCATTTTGATCCTGCCCTCGTTCCTCCTGATGTCCCTTTCAGTACAACCTCATTCAGTTATCCTAAAGAATCACTACAGGCTTGGTCAGGTCCGTTGGTCGAACCTGCTTCTGCCGGTGGTCCAAGACGGAAGAAGCATACTGGACTCGATGGGCGAGAAACTTCAAAATCCTATACAGGAAACCTTAGAGAGAAACATGACAATGCTCGTGTTAAGGGAAAGAGAAGTGCGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

588

Amino Acids

64.9

Weight (kDa)

9.44

Isoelectric Point (pI)

54.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	119 - 403	1e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	121 - 333	5.8e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001548)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54610 AT1G54610 AT1G54610 AT3G05050 AT3G05050 AT3G05050
fragaria_vesca	FvH4_4g17190 FvH4_4g17190 FvH4_4g17190 FvH4_4g17190 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100
malus_domestica	MD02G1048200.v1.1 MD04G1216500.v1.1 MD12G1233100.v1.1 MD13G1173900.v1.1 MD16G1175000.v1.1
prunus_persica	Prupe.1G142100_v2.0.a1 Prupe.1G142100_v2.0.a1 Prupe.6G336200_v2.0.a1 Prupe.6G336200_v2.0.a1
pyrus_communis	pycom04g19240 pycom12g21560
rosa_chinensis	RchiOBHm_Chr3g0452651 RchiOBHm_Chr4g0420991
rosa_laevigata	RLG00000007715 RLG00000025575
rosa_multiflora	Rmu_sc0006508.1_g000002 Rmu_ssc0000156.1_g000011
rosa_roxburghii	Rroxscaffold_5G00363340 Rroxscaffold_6G00429690
rosa_rugosa	Rorug02G0642200 Rorug02G0642300 Rorug02G0642300 Rorug02G0642400 Rorug04G0167500 Rorug04G0167500
rosa_samantha	Rh3AG044600 Rh3BG046900 Rh3CG045400 Rh3DG045800 Rh4AG229000 Rh4BG232000 Rh4CG243600 Rh4DG227400
rosa_wichuraiana	Rw3G003480 Rw4G019800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	908, 1639
AccB1I	GGYRCC	3 cut(s)	109, 266, 1431
AccI	GTMKAC	2 cut(s)	83, 1303
AccII	CGCG	1 cut(s)	262
AciI	CCGC	7 cut(s)	132, 278, 511, 932, 1403, 1481, 1757
AclWI	GGATC	7 cut(s)	528, 745, 800, 1154, 1317, 1525, 1529
AcoI	YGGCCR	2 cut(s)	279, 287
AcsI	RAATTY	2 cut(s)	498, 641
AcuI	CTGAAG	2 cut(s)	1002, 1394
AcyI	GRCGYC	3 cut(s)	267, 1275, 1432
AfaI	GTAC	6 cut(s)	249, 401, 581, 768, 970, 1570
AfiI	CCNNNNNNNGG	7 cut(s)	203, 239, 277, 589, 877, 1284, 1376
AflIII	ACRYGT	1 cut(s)	351
AgsI	TTSAA	2 cut(s)	1108, 1692
AhlI	ACTAGT	1 cut(s)	830
AjnI	CCWGG	4 cut(s)	293, 582, 961, 1326
AleI	CACNNNNGTG	1 cut(s)	708
AluBI	AGCT	9 cut(s)	26, 407, 614, 868, 944, 1187, 1298, 1313, 1408
AluI	AGCT	9 cut(s)	26, 407, 614, 868, 944, 1187, 1298, 1313, 1408
Alw21I	GWGCWC	2 cut(s)	699, 870
AlwI	GGATC	7 cut(s)	528, 745, 800, 1154, 1317, 1525, 1529
Ama87I	CYCGRG	4 cut(s)	232, 470, 863, 1071
AoxI	GGCC	3 cut(s)	279, 287, 1067
ApeKI	GCWGC	3 cut(s)	623, 1187, 1313
ApoI	RAATTY	2 cut(s)	498, 641
ArsI	GACNNNNNNTTYG	2 cut(s)	221, 253
Asp700I	GAANNNNTTC	2 cut(s)	1146, 1473
AspA2I	CCTAGG	1 cut(s)	1370
AspLEI	GCGC	3 cut(s)	269, 322, 1434
AspS9I	GGNCC	4 cut(s)	904, 1337, 1616, 1646
AsuC2I	CCSGG	1 cut(s)	1286
AsuHPI	GGTGA	3 cut(s)	200, 713, 1005
AvaI	CYCGRG	4 cut(s)	232, 470, 863, 1071
AvaII	GGWCC	4 cut(s)	904, 1337, 1616, 1646
AvrII	CCTAGG	1 cut(s)	1370
BalI	TGGCCA	1 cut(s)	289
BanI	GGYRCC	3 cut(s)	109, 266, 1431
BanII	GRGCYC	3 cut(s)	471, 870, 1369
BarI	GAAGNNNNNNTAC	2 cut(s)	10, 42
BauI	CACGAG	1 cut(s)	1734
Bbv12I	GWGCWC	2 cut(s)	699, 870
BbvI	GCAGC	3 cut(s)	610, 1174, 1300
BccI	CCATC	4 cut(s)	1129, 1328, 1487, 1670
BciT130I	CCWGG	4 cut(s)	295, 584, 963, 1328
BciVI	GTATCC	1 cut(s)	1263
BcnI	CCSGG	1 cut(s)	1286
BcuI	ACTAGT	1 cut(s)	830
BfaI	CTAG	6 cut(s)	492, 611, 831, 941, 1239, 1371
BfmI	CTRYAG	1 cut(s)	1601
BfoI	RGCGCY	3 cut(s)	270, 323, 1435
BfuAI	ACCTGC	2 cut(s)	908, 1639
BfuI	GTATCC	1 cut(s)	1263
BglI	GCCNNNNNGGC	2 cut(s)	275, 295
BisI	GCNGC	5 cut(s)	279, 624, 1188, 1314, 1758
BlnI	CCTAGG	1 cut(s)	1370
BlsI	GCNGC	5 cut(s)	280, 625, 1189, 1315, 1759
BmcAI	AGTACT	1 cut(s)	768
Bme1390I	CCNGG	5 cut(s)	295, 584, 963, 1286, 1328
Bme18I	GGWCC	4 cut(s)	904, 1337, 1616, 1646
BmeT110I	CYCGRG	4 cut(s)	232, 470, 863, 1071
BmgT120I	GGNCC	4 cut(s)	904, 1337, 1616, 1646
BmiI	GGNNCC	7 cut(s)	111, 268, 468, 1077, 1368, 1389, 1433
BmrFI	CCNGG	5 cut(s)	295, 584, 963, 1286, 1328
BmrI	ACTGGG	1 cut(s)	96
BmsI	GCATC	4 cut(s)	88, 682, 829, 1534
BmuI	ACTGGG	1 cut(s)	96
BpmI	CTGGAG	3 cut(s)	931, 1051, 1374
BpuEI	CTTGAG	1 cut(s)	713
BpuMI	CCSGG	1 cut(s)	1286
BsaHI	GRCGYC	3 cut(s)	267, 1275, 1432
BsaXI	ACNNNNNCTCC	4 cut(s)	904, 934, 1397, 1427
Bsc4I	CCNNNNNNNGG	7 cut(s)	203, 239, 277, 589, 877, 1284, 1376
Bse118I	RCCGGY	3 cut(s)	281, 1384, 1640
Bse1I	ACTGG	3 cut(s)	91, 1034, 1672
Bse3DI	GCAATG	1 cut(s)	400
BseBI	CCWGG	4 cut(s)	295, 584, 963, 1328
BseGI	GGATG	2 cut(s)	820, 1498
BseLI	CCNNNNNNNGG	7 cut(s)	203, 239, 277, 589, 877, 1284, 1376
BseMI	GCAATG	1 cut(s)	400
BseMII	CTCAG	2 cut(s)	36, 294
BseNI	ACTGG	3 cut(s)	91, 1034, 1672
BseRI	GAGGAG	3 cut(s)	76, 185, 777
BseX3I	CGGCCG	1 cut(s)	279
BseXI	GCAGC	3 cut(s)	610, 1174, 1300
BseYI	CCCAGC	2 cut(s)	614, 944
BsgI	GTGCAG	1 cut(s)	936
Bsh1236I	CGCG	1 cut(s)	262
Bsh1285I	CGRYCG	2 cut(s)	221, 282
BshFI	GGCC	3 cut(s)	281, 289, 1069
BshNI	GGYRCC	3 cut(s)	109, 266, 1431
BsiEI	CGRYCG	2 cut(s)	221, 282
BsiHKAI	GWGCWC	2 cut(s)	699, 870
BsiHKCI	CYCGRG	4 cut(s)	232, 470, 863, 1071
BsiSI	CCGG	4 cut(s)	282, 1285, 1385, 1641
BslFI	GGGAC	2 cut(s)	1301, 1544
BslI	CCNNNNNNNGG	7 cut(s)	203, 239, 277, 589, 877, 1284, 1376
BsmFI	GGGAC	2 cut(s)	1301, 1544
BsmI	GAATGC	1 cut(s)	1103
BsnI	GGCC	3 cut(s)	281, 289, 1069
BsoBI	CYCGRG	4 cut(s)	232, 470, 863, 1071
Bsp1286I	GDGCHC	4 cut(s)	471, 699, 870, 1369
Bsp1407I	TGTACA	1 cut(s)	399
Bsp143I	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
Bsp19I	CCATGG	1 cut(s)	181
BspACI	CCGC	7 cut(s)	132, 278, 511, 932, 1403, 1481, 1757
BspANI	GGCC	3 cut(s)	281, 289, 1069
BspCNI	CTCAG	2 cut(s)	37, 295
BspFNI	CGCG	1 cut(s)	262
BspLI	GGNNCC	7 cut(s)	111, 268, 468, 1077, 1368, 1389, 1433
BspMI	ACCTGC	2 cut(s)	908, 1639
BspPI	GGATC	7 cut(s)	528, 745, 800, 1154, 1317, 1525, 1529
BspT107I	GGYRCC	3 cut(s)	109, 266, 1431
BsrDI	GCAATG	1 cut(s)	400
BsrFI	RCCGGY	3 cut(s)	281, 1384, 1640
BsrGI	TGTACA	1 cut(s)	399
BsrI	ACTGG	3 cut(s)	91, 1034, 1672
BssAI	RCCGGY	3 cut(s)	281, 1384, 1640
BssMI	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
BssNI	GRCGYC	3 cut(s)	267, 1275, 1432
BssSI	CACGAG	1 cut(s)	1734
BssT1I	CCWWGG	2 cut(s)	181, 1370
Bst2BI	CACGAG	1 cut(s)	1734
Bst2UI	CCWGG	4 cut(s)	295, 584, 963, 1328
Bst4CI	ACNGT	4 cut(s)	701, 710, 725, 893
Bst6I	CTCTTC	1 cut(s)	1152
BstACI	GRCGYC	3 cut(s)	267, 1275, 1432
BstAUI	TGTACA	1 cut(s)	399
BstC8I	GCNNGC	6 cut(s)	283, 287, 621, 628, 1386, 1446
BstDEI	CTNAG	3 cut(s)	45, 303, 1712
BstDSI	CCRYGG	3 cut(s)	181, 338, 709
BstF5I	GGATG	2 cut(s)	820, 1498
BstFNI	CGCG	1 cut(s)	262
BstH2I	RGCGCY	3 cut(s)	270, 323, 1435
BstHHI	GCGC	3 cut(s)	269, 322, 1434
BstKTI	GATC	9 cut(s)	221, 523, 610, 740, 808, 1162, 1325, 1520, 1537
BstMBI	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
BstMCI	CGRYCG	2 cut(s)	221, 282
BstMWI	GCNNNNNNNGC	9 cut(s)	223, 275, 295, 620, 958, 1066, 1075, 1187, 1394
BstNI	CCWGG	4 cut(s)	295, 584, 963, 1328
BstNSI	RCATGY	3 cut(s)	312, 673, 1448
BstSCI	CCNGG	5 cut(s)	293, 582, 961, 1284, 1326
BstSFI	CTRYAG	1 cut(s)	1601
BstUI	CGCG	1 cut(s)	262
BstV1I	GCAGC	3 cut(s)	610, 1174, 1300
BstX2I	RGATCY	2 cut(s)	1322, 1517
BstXI	CCANNNNNNTGG	1 cut(s)	1334
BstYI	RGATCY	2 cut(s)	1322, 1517
BstZI	CGGCCG	1 cut(s)	279
BsuI	GTATCC	1 cut(s)	1263
BsuRI	GGCC	3 cut(s)	281, 289, 1069
BtgI	CCRYGG	3 cut(s)	181, 338, 709
BtsCI	GGATG	2 cut(s)	820, 1498
BtsIMutI	CAGTG	2 cut(s)	300, 697
BveI	ACCTGC	2 cut(s)	908, 1639
Cac8I	GCNNGC	6 cut(s)	283, 287, 621, 628, 1386, 1446
CfoI	GCGC	3 cut(s)	269, 322, 1434
Cfr10I	RCCGGY	3 cut(s)	281, 1384, 1640
Cfr13I	GGNCC	4 cut(s)	904, 1337, 1616, 1646
CseI	GACGC	1 cut(s)	1283
CsiI	ACCWGGT	1 cut(s)	582
Csp6I	GTAC	6 cut(s)	248, 400, 580, 767, 969, 1569
CspCI	CAANNNNNGTGG	2 cut(s)	988, 1023
CviQI	GTAC	6 cut(s)	248, 400, 580, 767, 969, 1569
DdeI	CTNAG	3 cut(s)	45, 303, 1712
DinI	GGCGCC	2 cut(s)	268, 1433
DpnI	GATC	9 cut(s)	220, 522, 609, 739, 807, 1161, 1324, 1519, 1536
DpnII	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
EaeI	YGGCCR	2 cut(s)	279, 287
EagI	CGGCCG	1 cut(s)	279
Eam1104I	CTCTTC	1 cut(s)	1152
EarI	CTCTTC	1 cut(s)	1152
EciI	GGCGGA	1 cut(s)	1470
Ecl136II	GAGCTC	1 cut(s)	868
EclXI	CGGCCG	1 cut(s)	279
Eco130I	CCWWGG	2 cut(s)	181, 1370
Eco147I	AGGCCT	1 cut(s)	1069
Eco24I	GRGCYC	3 cut(s)	471, 870, 1369
Eco47I	GGWCC	4 cut(s)	904, 1337, 1616, 1646
Eco52I	CGGCCG	1 cut(s)	279
Eco53kI	GAGCTC	1 cut(s)	868
Eco57I	CTGAAG	2 cut(s)	1002, 1394
Eco88I	CYCGRG	4 cut(s)	232, 470, 863, 1071
EcoICRI	GAGCTC	1 cut(s)	868
EcoRII	CCWGG	4 cut(s)	293, 582, 961, 1326
EcoT14I	CCWWGG	2 cut(s)	181, 1370
EcoT22I	ATGCAT	1 cut(s)	675
EcoT38I	GRGCYC	3 cut(s)	471, 870, 1369
EgeI	GGCGCC	2 cut(s)	268, 1433
EheI	GGCGCC	2 cut(s)	268, 1433
ErhI	CCWWGG	2 cut(s)	181, 1370
FalI	AAGNNNNNCTT	1 cut(s)	1744
FaqI	GGGAC	2 cut(s)	1301, 1544
FblI	GTMKAC	2 cut(s)	83, 1303
Fnu4HI	GCNGC	5 cut(s)	279, 624, 1188, 1314, 1758
FokI	GGATG	2 cut(s)	807, 1505
FriOI	GRGCYC	3 cut(s)	471, 870, 1369
Fsp4HI	GCNGC	5 cut(s)	279, 624, 1188, 1314, 1758
FspBI	CTAG	6 cut(s)	492, 611, 831, 941, 1239, 1371
GlaI	GCGC	3 cut(s)	268, 321, 1433
GluI	GCNGC	5 cut(s)	279, 624, 1188, 1314, 1758
GsaI	CCCAGC	2 cut(s)	618, 948
GsuI	CTGGAG	3 cut(s)	931, 1051, 1374
HaeII	RGCGCY	3 cut(s)	270, 323, 1435
HaeIII	GGCC	3 cut(s)	281, 289, 1069
HapII	CCGG	4 cut(s)	282, 1285, 1385, 1641
HgaI	GACGC	1 cut(s)	1283
HhaI	GCGC	3 cut(s)	269, 322, 1434
Hin1I	GRCGYC	3 cut(s)	267, 1275, 1432
Hin6I	GCGC	3 cut(s)	267, 320, 1432
HinP1I	GCGC	3 cut(s)	267, 320, 1432
HincII	GTYRAC	1 cut(s)	420
HindII	GTYRAC	1 cut(s)	420
HindIII	AAGCTT	1 cut(s)	24
HinfI	GANTC	6 cut(s)	36, 47, 80, 334, 1595, 1671
HpaII	CCGG	4 cut(s)	282, 1285, 1385, 1641
HphI	GGTGA	3 cut(s)	200, 713, 1005
Hpy166II	GTNNAC	7 cut(s)	84, 400, 420, 904, 1013, 1232, 1304
Hpy188I	TCNGA	5 cut(s)	46, 201, 1021, 1176, 1216
Hpy8I	GTNNAC	7 cut(s)	84, 400, 420, 904, 1013, 1232, 1304
HpyAV	CCTTC	9 cut(s)	46, 85, 109, 160, 207, 881, 1026, 1245, 1422
HpyCH4III	ACNGT	4 cut(s)	701, 710, 725, 893
HpyCH4IV	ACGT	4 cut(s)	353, 386, 557, 1171
HpyCH4V	TGCA	9 cut(s)	626, 673, 917, 924, 1055, 1154, 1190, 1448, 1454
HpyF10VI	GCNNNNNNNGC	9 cut(s)	223, 275, 295, 620, 958, 1066, 1075, 1187, 1394
HpyF3I	CTNAG	3 cut(s)	45, 303, 1712
HpySE526I	ACGT	4 cut(s)	353, 386, 557, 1171
Hsp92I	GRCGYC	3 cut(s)	267, 1275, 1432
HspAI	GCGC	3 cut(s)	267, 320, 1432
KasI	GGCGCC	2 cut(s)	266, 1431
KroI	GCCGGC	2 cut(s)	281, 1384
KroNI	GCCGGC	2 cut(s)	283, 1386
Kzo9I	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
LmnI	GCTCC	9 cut(s)	466, 601, 873, 979, 1075, 1372, 1393, 1405, 1496
Lsp1109I	GCAGC	3 cut(s)	610, 1174, 1300
LweI	GCATC	4 cut(s)	88, 682, 829, 1534
MabI	ACCWGGT	1 cut(s)	582
MaeI	CTAG	6 cut(s)	492, 611, 831, 941, 1239, 1371
MaeII	ACGT	4 cut(s)	353, 386, 557, 1171
MaeIII	GTNAC	6 cut(s)	553, 665, 701, 725, 841, 936
MalI	GATC	9 cut(s)	220, 522, 609, 739, 807, 1161, 1324, 1519, 1536
MboI	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
MboII	GAAGA	6 cut(s)	21, 1098, 1116, 1139, 1512, 1669
MfeI	CAATTG	1 cut(s)	1155
MflI	RGATCY	2 cut(s)	1322, 1517
MhlI	GDGCHC	4 cut(s)	471, 699, 870, 1369
MlsI	TGGCCA	1 cut(s)	289
MluNI	TGGCCA	1 cut(s)	289
Mly113I	GGCGCC	2 cut(s)	267, 1432
MlyI	GAGTC	4 cut(s)	45, 56, 89, 1665
MmeI	TCCRAC	2 cut(s)	522, 570
Mox20I	TGGCCA	1 cut(s)	289
Mph1103I	ATGCAT	1 cut(s)	675
MroNI	GCCGGC	2 cut(s)	281, 1384
MroXI	GAANNNNTTC	2 cut(s)	1146, 1473
MscI	TGGCCA	1 cut(s)	289
MseI	TTAA	4 cut(s)	639, 732, 771, 1740
MslI	CAYNNNNRTG	7 cut(s)	147, 186, 528, 708, 1449, 1491, 1728
Msp20I	TGGCCA	1 cut(s)	289
MspA1I	CMGCKG	1 cut(s)	1187
MspI	CCGG	4 cut(s)	282, 1285, 1385, 1641
MspR9I	CCNGG	5 cut(s)	295, 584, 963, 1286, 1328
MunI	CAATTG	1 cut(s)	1155
Mva1269I	GAATGC	1 cut(s)	1103
MvaI	CCWGG	4 cut(s)	295, 584, 963, 1328
MvnI	CGCG	1 cut(s)	262
MwoI	GCNNNNNNNGC	9 cut(s)	223, 275, 295, 620, 958, 1066, 1075, 1187, 1394
NaeI	GCCGGC	2 cut(s)	283, 1386
NarI	GGCGCC	2 cut(s)	267, 1432
NciI	CCSGG	1 cut(s)	1286
NcoI	CCATGG	1 cut(s)	181
NdeII	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
NgoMIV	GCCGGC	2 cut(s)	281, 1384
NlaIV	GGNNCC	7 cut(s)	111, 268, 468, 1077, 1368, 1389, 1433
NmeAIII	GCCGAG	1 cut(s)	160
NmuCI	GTSAC	2 cut(s)	701, 725
NsiI	ATGCAT	1 cut(s)	675
NspI	RCATGY	3 cut(s)	312, 673, 1448
OliI	CACNNNNGTG	1 cut(s)	708
PaeI	GCATGC	1 cut(s)	1448
PceI	AGGCCT	1 cut(s)	1069
PcsI	WCGNNNNNNNCGW	1 cut(s)	1306
PctI	GAATGC	1 cut(s)	1103
PdiI	GCCGGC	2 cut(s)	283, 1386
PdmI	GAANNNNTTC	2 cut(s)	1146, 1473
PfeI	GAWTC	2 cut(s)	334, 1595
PkrI	GCNGC	5 cut(s)	280, 625, 1189, 1315, 1759
Ple19I	CGATCG	1 cut(s)	221
PleI	GAGTC	4 cut(s)	44, 55, 88, 1665
PluTI	GGCGCC	2 cut(s)	270, 1435
PpsI	GAGTC	4 cut(s)	44, 55, 88, 1665
Psp124BI	GAGCTC	1 cut(s)	870
Psp6I	CCWGG	4 cut(s)	293, 582, 961, 1326
PspFI	CCCAGC	2 cut(s)	614, 944
PspGI	CCWGG	4 cut(s)	293, 582, 961, 1326
PspN4I	GGNNCC	7 cut(s)	111, 268, 468, 1077, 1368, 1389, 1433
PspPI	GGNCC	4 cut(s)	904, 1337, 1616, 1646
PsuI	RGATCY	2 cut(s)	1322, 1517
PvuI	CGATCG	1 cut(s)	221
PvuII	CAGCTG	1 cut(s)	1187
RsaI	GTAC	6 cut(s)	249, 401, 581, 768, 970, 1570
RsaNI	GTAC	6 cut(s)	248, 400, 580, 767, 969, 1569
RseI	CAYNNNNRTG	7 cut(s)	147, 186, 528, 708, 1449, 1491, 1728
SacI	GAGCTC	1 cut(s)	870
SaqAI	TTAA	4 cut(s)	639, 732, 771, 1740
SatI	GCNGC	5 cut(s)	279, 624, 1188, 1314, 1758
Sau3AI	GATC	9 cut(s)	218, 520, 607, 737, 805, 1159, 1322, 1517, 1534
Sau96I	GGNCC	4 cut(s)	904, 1337, 1616, 1646
ScaI	AGTACT	1 cut(s)	768
SchI	GAGTC	4 cut(s)	45, 56, 89, 1665
ScrFI	CCNGG	5 cut(s)	295, 584, 963, 1286, 1328
SduI	GDGCHC	4 cut(s)	471, 699, 870, 1369
SexAI	ACCWGGT	1 cut(s)	582
SfaNI	GCATC	4 cut(s)	88, 682, 829, 1534
SfcI	CTRYAG	1 cut(s)	1601
SfoI	GGCGCC	2 cut(s)	268, 1433
SinI	GGWCC	4 cut(s)	904, 1337, 1616, 1646
SmiMI	CAYNNNNRTG	7 cut(s)	147, 186, 528, 708, 1449, 1491, 1728
SmlI	CTYRAG	1 cut(s)	692
SmoI	CTYRAG	1 cut(s)	692
SpeI	ACTAGT	1 cut(s)	830
SphI	GCATGC	1 cut(s)	1448
SseBI	AGGCCT	1 cut(s)	1069
SsiI	CCGC	7 cut(s)	132, 278, 511, 932, 1403, 1481, 1757
SspDI	GGCGCC	2 cut(s)	266, 1431
SspI	AATATT	1 cut(s)	1420
SspMI	CTAG	6 cut(s)	492, 611, 831, 941, 1239, 1371
SstI	GAGCTC	1 cut(s)	870
StuI	AGGCCT	1 cut(s)	1069
StyD4I	CCNGG	5 cut(s)	293, 582, 961, 1284, 1326
StyI	CCWWGG	2 cut(s)	181, 1370
TaaI	ACNGT	4 cut(s)	701, 710, 725, 893
TaiI	ACGT	4 cut(s)	356, 389, 560, 1174
TaqI	TCGA	6 cut(s)	50, 221, 856, 1140, 1626, 1674
TatI	WGTACW	3 cut(s)	399, 766, 1568
TauI	GCSGC	2 cut(s)	281, 1760
TfiI	GAWTC	2 cut(s)	334, 1595
Tru1I	TTAA	4 cut(s)	639, 732, 771, 1740
Tru9I	TTAA	4 cut(s)	639, 732, 771, 1740
TscAI	CASTG	2 cut(s)	307, 704
TseFI	GTSAC	2 cut(s)	701, 725
TseI	GCWGC	3 cut(s)	623, 1187, 1313
Tsp45I	GTSAC	2 cut(s)	701, 725
TspDTI	ATGAA	4 cut(s)	131, 1038, 1116, 1308
TspGWI	ACGGA	3 cut(s)	355, 1608, 1669
TspRI	CASTG	2 cut(s)	307, 704
VpaK11BI	GGWCC	4 cut(s)	904, 1337, 1616, 1646
XapI	RAATTY	2 cut(s)	498, 641
XceI	RCATGY	3 cut(s)	312, 673, 1448
XmaJI	CCTAGG	1 cut(s)	1370
XmiI	GTMKAC	2 cut(s)	83, 1303
XmnI	GAANNNNTTC	2 cut(s)	1146, 1473
XspI	CTAG	6 cut(s)	492, 611, 831, 941, 1239, 1371
ZrmI	AGTACT	1 cut(s)	768
Zsp2I	ATGCAT	1 cut(s)	675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.