Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001548

Physical Location & Seq

Genomic Coordinates Forward (+)

14318022 .. 14321924

Gene Structure 3903 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1173900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGGGGTGCGTCTTCAGCAGACATCCGTCCGCAAACCCTGTTGGGAATGACGGAGATGGTGAAATTAGGGTTTCCAGGAAAGCAGAGTCCACTGCCGCCGCCGCCGGCGAGGTCAGTGAGGTGAGGAATGGTGTGAATAATAGTATTAGTAACGATGATAGTAACAGTAATCGGAAGGAGAATGAGGAGGAGAGGAGTTCGAGGCCTAAAGGGGAGAAGAGACGCTCCTCCAAGCCCAATCCTAGGCTCAGCAATCCGCCCAAGCACCTCCTTGGCGAGCAGGTCGCCGCCGGTTGGCCCTCCTGGCTCTCCGCCGTCGCCGGCGAGGCCATCAATGGCTGGACGCCCCGCCGCGCCGATACATTCGAGAAGCTTGATAAGATTGGGCAAGGGACGTATAGTAATGTGTACAAAGCTAGGGATGCTTTGTCGGGGAAAATTGTTGCGCTGAAGAAAGTTCGGTTTGATAATTTGGAGCCCGAGAGTGTAAAGTTCATGGCCAGGGAGATCCTCATTCTGCGCCGTCTTGATCATCCTAATGTTGTAAAATTGGAAGGTTTAGTGACCTCAAGGATGTCATGTAGTTTGTACCTTGTATTTGAATATATGGAGCATGATTTGGCAGGACTTGCTGCAAGCCCTGCAATCAAGTTCACAGAGCCTCAGGTTAAGTGTTATATGAACCAACTATTATCAGGGCTGGAACACTGTCACAACCGCTATGTGCTTCATCGTGATATCAAAGGGTCCAATCTTCTTATTGACAATGGTGGTGTCCTGAAGATTGCTGATTTTGGATTGGCTTCCTTCTTTGACCCTAATTATAAGCAACCAATGACTAGTAGAGTGGTCACTTTATGGTATCGACCACCAGAGCTTCTTCTAGGTGCCACTGATTATGGTGTAGGTGTGGACCTCTGGAGTGCTGGCTGCATTTTAGCTGAGCTGTTGGCCGGAAAACCGATCATGCCAGGTCGCACAGAAGTGGAGCAGCTACACAAGATATTTAAGTTATGCGGTTCACCTTCTGATGATTACTGGAAAAAGTCAAAGTTGCCTCATGCAACCATTTTTAAGCCTCAGCAATCATATAAACGATGCATTGCCGAGACATTTAAAGATTTTCCACCATCATCATTGCCACTAATTGAGACACTCCTTGCAATTGACCCAGCTGAGCGTCGGACTGCCACTGCTGCATTGAGGAATGAATTCTTCACAACCAAACCTTATGCCTGCGAACCTTCTAGCCTCCCAAAATATCCACCCAGCAAAGAGATGGATGCTAAGCTACGGGATGAAGAAGCTAGAAGATTGAGAGCTGCTGGCAGAGCTAACGCAGATGGTGTGAGGAAATCTCGACCACGTGATCGCAAGGGAATTCCTGCTCCAGAAGCCAATGCTGAACTTCAAGCCAATCTTGATAGGCGGCGTCTAATCACACATGCAAACGCAAAGAGCAAGAGTGAGAAGTTTCCTCCTCCACACCAAGACGGAACACTTGGCTATCCTTTGGGTTCATCAAATCACATTGATCCTGCTTTTGATCCTCCGGATGTTCCATTTAGTTCCACAAACTTTTCGTATTCAAAAGAATCCATCCAAACTTGGTCTGGCCCATTGGTAGACCCTGCTGCAGTAGGGGCTCCAAGGAGAAAGAAACACTCATCCGGACAAATTCATTCACTGTCGAAGCCCTCGAGGAAAGACTCAAAACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

574

Amino Acids

63.53

Weight (kDa)

9.42

Isoelectric Point (pI)

50.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	122 - 406	1.4e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	125 - 335	9.3e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001548)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54610 AT1G54610 AT1G54610 AT3G05050 AT3G05050 AT3G05050
fragaria_vesca	FvH4_4g17190 FvH4_4g17190 FvH4_4g17190 FvH4_4g17190 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100
malus_domestica	MD02G1048200.v1.1 MD04G1216500.v1.1 MD12G1233100.v1.1 MD13G1173900.v1.1 MD16G1175000.v1.1
prunus_persica	Prupe.1G142100_v2.0.a1 Prupe.1G142100_v2.0.a1 Prupe.6G336200_v2.0.a1 Prupe.6G336200_v2.0.a1
pyrus_communis	pycom04g19240 pycom12g21560
rosa_chinensis	RchiOBHm_Chr3g0452651 RchiOBHm_Chr4g0420991
rosa_laevigata	RLG00000007715 RLG00000025575
rosa_multiflora	Rmu_sc0006508.1_g000002 Rmu_ssc0000156.1_g000011
rosa_roxburghii	Rroxscaffold_5G00363340 Rroxscaffold_6G00429690
rosa_rugosa	Rorug02G0642200 Rorug02G0642300 Rorug02G0642300 Rorug02G0642400 Rorug04G0167500 Rorug04G0167500
rosa_samantha	Rh3AG044600 Rh3BG046900 Rh3CG045400 Rh3DG045800 Rh4AG229000 Rh4BG232000 Rh4CG243600 Rh4DG227400
rosa_wichuraiana	Rw3G003480 Rw4G019800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	825
AbsI	CCTCGAGG	1 cut(s)	1699
Acc36I	ACCTGC	1 cut(s)	271
AccB1I	GGYRCC	1 cut(s)	887
AccI	GTMKAC	1 cut(s)	1626
AccII	CGCG	1 cut(s)	354
AccIII	TCCGGA	2 cut(s)	1552, 1670
AclWI	GGATC	3 cut(s)	502, 1529, 1541
AcoI	YGGCCR	2 cut(s)	498, 951
AcsI	RAATTY	3 cut(s)	1211, 1380, 1677
AcuI	CTGAAG	2 cut(s)	470, 800
AcvI	CACGTG	1 cut(s)	1367
AcyI	GRCGYC	2 cut(s)	344, 1432
AfaI	GTAC	2 cut(s)	410, 590
AfiI	CCNNNNNNNGG	2 cut(s)	243, 294
AgsI	TTSAA	3 cut(s)	602, 1412, 1590
AhlI	ACTAGT	1 cut(s)	839
AjnI	CCWGG	4 cut(s)	74, 302, 500, 970
AleI	CACNNNNGTG	1 cut(s)	983
Alw26I	GTCTC	3 cut(s)	214, 1103, 1145
AlwI	GGATC	3 cut(s)	502, 1529, 1541
Ama87I	CYCGRG	2 cut(s)	479, 1699
Aor13HI	TCCGGA	2 cut(s)	1552, 1670
AoxI	GGCC	6 cut(s)	203, 296, 327, 498, 951, 1615
ApeKI	GCWGC	6 cut(s)	632, 930, 991, 1196, 1322, 1634
ApoI	RAATTY	3 cut(s)	1211, 1380, 1677
Asp700I	GAANNNNTTC	1 cut(s)	1211
AspA2I	CCTAGG	1 cut(s)	242
AspLEI	GCGC	3 cut(s)	356, 448, 522
AspS9I	GGNCC	4 cut(s)	297, 747, 913, 1616
AsuHPI	GGTGA	3 cut(s)	71, 133, 1014
AvaI	CYCGRG	2 cut(s)	479, 1699
AvaII	GGWCC	2 cut(s)	747, 913
AvrII	CCTAGG	1 cut(s)	242
AxyI	CCTNAGG	1 cut(s)	663
BalI	TGGCCA	1 cut(s)	500
BanI	GGYRCC	1 cut(s)	887
BanII	GRGCYC	2 cut(s)	480, 1648
BbrPI	CACGTG	1 cut(s)	1367
BbsI	GAAGAC	1 cut(s)	4
BbvCI	CCTCAGC	1 cut(s)	1080
BbvI	GCAGC	6 cut(s)	619, 917, 1003, 1183, 1309, 1621
BccI	CCATC	6 cut(s)	50, 338, 1138, 1273, 1337, 1607
BceAI	ACGGC	2 cut(s)	299, 507
BciT130I	CCWGG	4 cut(s)	76, 304, 502, 972
BclI	TGATCA	1 cut(s)	529
BcoDI	GTCTC	3 cut(s)	214, 1103, 1145
BcuI	ACTAGT	1 cut(s)	839
BfaI	CTAG	6 cut(s)	243, 417, 840, 884, 1248, 1308
BfmI	CTRYAG	1 cut(s)	1635
BfuAI	ACCTGC	1 cut(s)	271
BglI	GCCNNNNNGGC	2 cut(s)	304, 326
BlnI	CCTAGG	1 cut(s)	242
BlpI	GCTNAGC	4 cut(s)	248, 942, 1176, 1287
Bme1390I	CCNGG	4 cut(s)	76, 304, 502, 972
Bme18I	GGWCC	2 cut(s)	747, 913
BmeT110I	CYCGRG	2 cut(s)	479, 1699
BmgT120I	GGNCC	4 cut(s)	297, 747, 913, 1616
BmiI	GGNNCC	4 cut(s)	477, 748, 889, 1647
BmrFI	CCNGG	4 cut(s)	76, 304, 502, 972
BmsI	GCATC	3 cut(s)	412, 1088, 1273
BoxI	GACNNNNGTC	1 cut(s)	25
BpiI	GAAGAC	1 cut(s)	4
BplI	GAGNNNNNCTC	2 cut(s)	1342, 1374
BpmI	CTGGAG	2 cut(s)	940, 1374
Bpu10I	CCTNAGC	1 cut(s)	1080
Bpu1102I	GCTNAGC	4 cut(s)	248, 942, 1176, 1287
BpuEI	CTTGAG	1 cut(s)	553
BsaAI	YACGTR	1 cut(s)	1367
BsaHI	GRCGYC	2 cut(s)	344, 1432
BsaJI	CCNNGG	4 cut(s)	242, 271, 501, 1649
BsaWI	WCCGGW	2 cut(s)	1552, 1670
Bsc4I	CCNNNNNNNGG	2 cut(s)	243, 294
Bse118I	RCCGGY	3 cut(s)	104, 290, 320
Bse1I	ACTGG	1 cut(s)	1043
Bse21I	CCTNAGG	1 cut(s)	663
Bse3DI	GCAATG	2 cut(s)	1101, 1136
BseAI	TCCGGA	2 cut(s)	1552, 1670
BseBI	CCWGG	4 cut(s)	76, 304, 502, 972
BseDI	CCNNGG	4 cut(s)	242, 271, 501, 1649
BseGI	GGATG	9 cut(s)	22, 427, 532, 579, 1288, 1303, 1561, 1599, 1667
BseLI	CCNNNNNNNGG	2 cut(s)	243, 294
BseMI	GCAATG	2 cut(s)	1101, 1136
BseMII	CTCAG	5 cut(s)	262, 677, 933, 1094, 1167
BseNI	ACTGG	1 cut(s)	1043
BseRI	GAGGAG	5 cut(s)	200, 203, 208, 217, 1470
BseXI	GCAGC	6 cut(s)	619, 917, 1003, 1183, 1309, 1621
BseYI	CCCAGC	2 cut(s)	1171, 1268
Bsh1236I	CGCG	1 cut(s)	354
BshFI	GGCC	6 cut(s)	205, 298, 329, 500, 953, 1617
BshNI	GGYRCC	1 cut(s)	887
BsiHKCI	CYCGRG	2 cut(s)	479, 1699
BsiSI	CCGG	6 cut(s)	105, 291, 321, 954, 1553, 1671
BslFI	GGGAC	1 cut(s)	406
BslI	CCNNNNNNNGG	2 cut(s)	243, 294
BsmAI	GTCTC	3 cut(s)	214, 1103, 1145
BsmBI	CGTCTC	1 cut(s)	214
BsmFI	GGGAC	1 cut(s)	406
BsnI	GGCC	6 cut(s)	205, 298, 329, 500, 953, 1617
BsoBI	CYCGRG	2 cut(s)	479, 1699
Bsp1286I	GDGCHC	2 cut(s)	480, 1648
Bsp13I	TCCGGA	2 cut(s)	1552, 1670
Bsp1407I	TGTACA	1 cut(s)	408
Bsp143I	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
Bsp1720I	GCTNAGC	4 cut(s)	248, 942, 1176, 1287
BspANI	GGCC	6 cut(s)	205, 298, 329, 500, 953, 1617
BspCNI	CTCAG	5 cut(s)	261, 676, 934, 1093, 1168
BspEI	TCCGGA	2 cut(s)	1552, 1670
BspFNI	CGCG	1 cut(s)	354
BspLI	GGNNCC	4 cut(s)	477, 748, 889, 1647
BspMAI	CTGCAG	1 cut(s)	1639
BspMI	ACCTGC	1 cut(s)	271
BspPI	GGATC	3 cut(s)	502, 1529, 1541
BspT107I	GGYRCC	1 cut(s)	887
BsrDI	GCAATG	2 cut(s)	1101, 1136
BsrFI	RCCGGY	3 cut(s)	104, 290, 320
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	1 cut(s)	1043
BssAI	RCCGGY	3 cut(s)	104, 290, 320
BssECI	CCNNGG	4 cut(s)	242, 271, 501, 1649
BssMI	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
BssNI	GRCGYC	2 cut(s)	344, 1432
BssT1I	CCWWGG	3 cut(s)	242, 271, 1649
Bst2UI	CCWGG	4 cut(s)	76, 304, 502, 972
Bst4CI	ACNGT	3 cut(s)	167, 710, 1689
Bst6I	CTCTTC	1 cut(s)	212
BstACI	GRCGYC	2 cut(s)	344, 1432
BstAPI	GCANNNNNTGC	2 cut(s)	629, 641
BstAUI	TGTACA	1 cut(s)	408
BstBAI	YACGTR	1 cut(s)	1367
BstC8I	GCNNGC	7 cut(s)	106, 278, 322, 637, 928, 1237, 1327
BstDEI	CTNAG	6 cut(s)	248, 663, 942, 1080, 1176, 1287
BstF5I	GGATG	9 cut(s)	22, 427, 532, 579, 1288, 1303, 1561, 1599, 1667
BstFNI	CGCG	1 cut(s)	354
BstHHI	GCGC	3 cut(s)	356, 448, 522
BstKTI	GATC	6 cut(s)	510, 532, 966, 1372, 1537, 1549
BstMAI	GTCTC	3 cut(s)	214, 1103, 1145
BstMBI	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
BstNI	CCWGG	4 cut(s)	76, 304, 502, 972
BstNSI	RCATGY	1 cut(s)	1448
BstPAI	GACNNNNGTC	1 cut(s)	25
BstSCI	CCNGG	4 cut(s)	74, 302, 500, 970
BstSFI	CTRYAG	1 cut(s)	1635
BstUI	CGCG	1 cut(s)	354
BstV1I	GCAGC	6 cut(s)	619, 917, 1003, 1183, 1309, 1621
BstV2I	GAAGAC	1 cut(s)	4
BstX2I	RGATCY	1 cut(s)	507
BstYI	RGATCY	1 cut(s)	507
Bsu36I	CCTNAGG	1 cut(s)	663
BsuRI	GGCC	6 cut(s)	205, 298, 329, 500, 953, 1617
BtsCI	GGATG	9 cut(s)	22, 427, 532, 579, 1288, 1303, 1561, 1599, 1667
BtsI	GCAGTG	2 cut(s)	90, 1191
BtsIMutI	CAGTG	6 cut(s)	90, 121, 706, 891, 1191, 1685
BveI	ACCTGC	1 cut(s)	271
Cac8I	GCNNGC	7 cut(s)	106, 278, 322, 637, 928, 1237, 1327
CfoI	GCGC	3 cut(s)	356, 448, 522
Cfr10I	RCCGGY	3 cut(s)	104, 290, 320
Cfr13I	GGNCC	4 cut(s)	297, 747, 913, 1616
CseI	GACGC	4 cut(s)	231, 352, 1169, 1421
Csp6I	GTAC	2 cut(s)	409, 589
CviAII	CATG	6 cut(s)	496, 579, 614, 967, 1061, 1445
CviQI	GTAC	2 cut(s)	409, 589
DdeI	CTNAG	6 cut(s)	248, 663, 942, 1080, 1176, 1287
DpnI	GATC	6 cut(s)	509, 531, 965, 1371, 1536, 1548
DpnII	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
DraI	TTTAAA	1 cut(s)	1117
EaeI	YGGCCR	2 cut(s)	498, 951
Eam1104I	CTCTTC	1 cut(s)	212
EarI	CTCTTC	1 cut(s)	212
EciI	GGCGGA	2 cut(s)	246, 301
Eco130I	CCWWGG	3 cut(s)	242, 271, 1649
Eco147I	AGGCCT	1 cut(s)	205
Eco24I	GRGCYC	2 cut(s)	480, 1648
Eco32I	GATATC	1 cut(s)	739
Eco47I	GGWCC	2 cut(s)	747, 913
Eco57I	CTGAAG	2 cut(s)	470, 800
Eco72I	CACGTG	1 cut(s)	1367
Eco81I	CCTNAGG	1 cut(s)	663
Eco88I	CYCGRG	2 cut(s)	479, 1699
EcoRI	GAATTC	2 cut(s)	1211, 1380
EcoRII	CCWGG	4 cut(s)	74, 302, 500, 970
EcoRV	GATATC	1 cut(s)	739
EcoT14I	CCWWGG	3 cut(s)	242, 271, 1649
EcoT22I	ATGCAT	1 cut(s)	1103
EcoT38I	GRGCYC	2 cut(s)	480, 1648
ErhI	CCWWGG	3 cut(s)	242, 271, 1649
Esp3I	CGTCTC	1 cut(s)	214
FaeI	CATG	6 cut(s)	499, 582, 617, 970, 1064, 1448
FalI	AAGNNNNNCTT	2 cut(s)	1404, 1436
FaqI	GGGAC	1 cut(s)	406
FatI	CATG	6 cut(s)	495, 578, 613, 966, 1060, 1444
FauI	CCCGC	1 cut(s)	356
FbaI	TGATCA	1 cut(s)	529
FblI	GTMKAC	1 cut(s)	1626
FokI	GGATG	9 cut(s)	9, 434, 519, 586, 1295, 1310, 1568, 1586, 1654
FriOI	GRGCYC	2 cut(s)	480, 1648
FspBI	CTAG	6 cut(s)	243, 417, 840, 884, 1248, 1308
GlaI	GCGC	3 cut(s)	355, 447, 521
GsaI	CCCAGC	2 cut(s)	1175, 1272
GsuI	CTGGAG	2 cut(s)	940, 1374
HaeIII	GGCC	6 cut(s)	205, 298, 329, 500, 953, 1617
HapII	CCGG	6 cut(s)	105, 291, 321, 954, 1553, 1671
HgaI	GACGC	4 cut(s)	231, 352, 1169, 1421
HhaI	GCGC	3 cut(s)	356, 448, 522
Hin1I	GRCGYC	2 cut(s)	344, 1432
Hin1II	CATG	6 cut(s)	499, 582, 617, 970, 1064, 1448
Hin6I	GCGC	3 cut(s)	354, 446, 520
HinP1I	GCGC	3 cut(s)	354, 446, 520
HindIII	AAGCTT	1 cut(s)	371
HinfI	GANTC	3 cut(s)	86, 1595, 1709
HpaII	CCGG	6 cut(s)	105, 291, 321, 954, 1553, 1671
HphI	GGTGA	3 cut(s)	71, 133, 1014
Hpy166II	GTNNAC	6 cut(s)	90, 409, 654, 913, 1022, 1627
Hpy188I	TCNGA	3 cut(s)	174, 1030, 1185
Hpy8I	GTNNAC	6 cut(s)	90, 409, 654, 913, 1022, 1627
Hpy99I	CGWCG	2 cut(s)	320, 1185
HpyAV	CCTTC	5 cut(s)	169, 548, 817, 1035, 1254
HpyCH4III	ACNGT	3 cut(s)	167, 710, 1689
HpyCH4IV	ACGT	2 cut(s)	395, 1366
HpyCH4V	TGCA	9 cut(s)	635, 644, 933, 1064, 1101, 1163, 1199, 1448, 1637
HpyF3I	CTNAG	6 cut(s)	248, 663, 942, 1080, 1176, 1287
HpySE526I	ACGT	2 cut(s)	395, 1366
Hsp92I	GRCGYC	2 cut(s)	344, 1432
Hsp92II	CATG	6 cut(s)	499, 582, 617, 970, 1064, 1448
HspAI	GCGC	3 cut(s)	354, 446, 520
Kpn2I	TCCGGA	2 cut(s)	1552, 1670
KroI	GCCGGC	2 cut(s)	104, 320
KroNI	GCCGGC	2 cut(s)	106, 322
Ksp22I	TGATCA	1 cut(s)	529
Kzo9I	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
LmnI	GCTCC	6 cut(s)	230, 475, 610, 988, 1393, 1651
Lsp1109I	GCAGC	6 cut(s)	619, 917, 1003, 1183, 1309, 1621
LweI	GCATC	3 cut(s)	412, 1088, 1273
MaeI	CTAG	6 cut(s)	243, 417, 840, 884, 1248, 1308
MaeII	ACGT	2 cut(s)	395, 1366
MaeIII	GTNAC	5 cut(s)	149, 161, 562, 710, 850
MalI	GATC	6 cut(s)	509, 531, 965, 1371, 1536, 1548
MboI	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
MboII	GAAGA	9 cut(s)	4, 229, 463, 746, 793, 872, 1207, 1313, 1323
MfeI	CAATTG	1 cut(s)	1164
MflI	RGATCY	1 cut(s)	507
MhlI	GDGCHC	2 cut(s)	480, 1648
MlsI	TGGCCA	1 cut(s)	500
MluNI	TGGCCA	1 cut(s)	500
MlyI	GAGTC	2 cut(s)	95, 1703
MmeI	TCCRAC	1 cut(s)	1163
Mox20I	TGGCCA	1 cut(s)	500
Mph1103I	ATGCAT	1 cut(s)	1103
MreI	CGCCGGCG	2 cut(s)	104, 320
MroI	TCCGGA	2 cut(s)	1552, 1670
MroNI	GCCGGC	2 cut(s)	104, 320
MroXI	GAANNNNTTC	1 cut(s)	1211
MscI	TGGCCA	1 cut(s)	500
MseI	TTAA	4 cut(s)	669, 1008, 1074, 1116
MslI	CAYNNNNRTG	2 cut(s)	537, 983
Msp20I	TGGCCA	1 cut(s)	500
MspA1I	CMGCKG	1 cut(s)	1175
MspI	CCGG	6 cut(s)	105, 291, 321, 954, 1553, 1671
MspR9I	CCNGG	4 cut(s)	76, 304, 502, 972
MunI	CAATTG	1 cut(s)	1164
MvaI	CCWGG	4 cut(s)	76, 304, 502, 972
MvnI	CGCG	1 cut(s)	354
NaeI	GCCGGC	2 cut(s)	106, 322
NdeII	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
NgoMIV	GCCGGC	2 cut(s)	104, 320
NlaIII	CATG	6 cut(s)	499, 582, 617, 970, 1064, 1448
NlaIV	GGNNCC	4 cut(s)	477, 748, 889, 1647
NmeAIII	GCCGAG	1 cut(s)	1132
NmuCI	GTSAC	3 cut(s)	562, 710, 850
NsiI	ATGCAT	1 cut(s)	1103
NspI	RCATGY	1 cut(s)	1448
OliI	CACNNNNGTG	1 cut(s)	983
PaeR7I	CTCGAG	1 cut(s)	1699
PceI	AGGCCT	1 cut(s)	205
PcsI	WCGNNNNNNNCGW	1 cut(s)	1697
PdiI	GCCGGC	2 cut(s)	106, 322
PdmI	GAANNNNTTC	1 cut(s)	1211
PfeI	GAWTC	1 cut(s)	1595
PfoI	TCCNGGA	1 cut(s)	74
PleI	GAGTC	2 cut(s)	94, 1703
PmaCI	CACGTG	1 cut(s)	1367
PmlI	CACGTG	1 cut(s)	1367
PpsI	GAGTC	2 cut(s)	94, 1703
Ppu21I	YACGTR	1 cut(s)	1367
PshAI	GACNNNNGTC	1 cut(s)	25
PsiI	TTATAA	1 cut(s)	825
Psp6I	CCWGG	4 cut(s)	74, 302, 500, 970
PspCI	CACGTG	1 cut(s)	1367
PspFI	CCCAGC	2 cut(s)	1171, 1268
PspGI	CCWGG	4 cut(s)	74, 302, 500, 970
PspN4I	GGNNCC	4 cut(s)	477, 748, 889, 1647
PspPI	GGNCC	4 cut(s)	297, 747, 913, 1616
PspXI	VCTCGAGB	1 cut(s)	1699
PstI	CTGCAG	1 cut(s)	1639
PsuI	RGATCY	1 cut(s)	507
PvuII	CAGCTG	1 cut(s)	1175
RsaI	GTAC	2 cut(s)	410, 590
RsaNI	GTAC	2 cut(s)	409, 589
RseI	CAYNNNNRTG	2 cut(s)	537, 983
SaqAI	TTAA	4 cut(s)	669, 1008, 1074, 1116
Sau3AI	GATC	6 cut(s)	507, 529, 963, 1369, 1534, 1546
Sau96I	GGNCC	4 cut(s)	297, 747, 913, 1616
SchI	GAGTC	2 cut(s)	95, 1703
ScrFI	CCNGG	4 cut(s)	76, 304, 502, 972
SduI	GDGCHC	2 cut(s)	480, 1648
SfaNI	GCATC	3 cut(s)	412, 1088, 1273
SfcI	CTRYAG	1 cut(s)	1635
Sfr274I	CTCGAG	1 cut(s)	1699
SgrAI	CRCCGGYG	2 cut(s)	104, 320
SinI	GGWCC	2 cut(s)	747, 913
SlaI	CTCGAG	1 cut(s)	1699
SmiMI	CAYNNNNRTG	2 cut(s)	537, 983
SmlI	CTYRAG	2 cut(s)	568, 1699
SmoI	CTYRAG	2 cut(s)	568, 1699
SpeI	ACTAGT	1 cut(s)	839
SseBI	AGGCCT	1 cut(s)	205
SspMI	CTAG	6 cut(s)	243, 417, 840, 884, 1248, 1308
StuI	AGGCCT	1 cut(s)	205
StyD4I	CCNGG	4 cut(s)	74, 302, 500, 970
StyI	CCWWGG	3 cut(s)	242, 271, 1649
TaaI	ACNGT	3 cut(s)	167, 710, 1689
TaiI	ACGT	2 cut(s)	398, 1369
TaqI	TCGA	6 cut(s)	200, 366, 865, 1360, 1691, 1700
TatI	WGTACW	1 cut(s)	408
TauI	GCSGC	6 cut(s)	98, 101, 104, 290, 354, 1432
TfiI	GAWTC	1 cut(s)	1595
Tru1I	TTAA	4 cut(s)	669, 1008, 1074, 1116
Tru9I	TTAA	4 cut(s)	669, 1008, 1074, 1116
TscAI	CASTG	6 cut(s)	97, 121, 713, 898, 1198, 1692
TseFI	GTSAC	3 cut(s)	562, 710, 850
TseI	GCWGC	6 cut(s)	632, 930, 991, 1196, 1322, 1634
Tsp45I	GTSAC	3 cut(s)	562, 710, 850
TspDTI	ATGAA	7 cut(s)	484, 695, 719, 1224, 1314, 1509, 1670
TspGWI	ACGGA	3 cut(s)	15, 66, 1509
TspRI	CASTG	6 cut(s)	97, 121, 713, 898, 1198, 1692
VpaK11BI	GGWCC	2 cut(s)	747, 913
XapI	RAATTY	3 cut(s)	1211, 1380, 1677
XceI	RCATGY	1 cut(s)	1448
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1276, 1610
XhoI	CTCGAG	1 cut(s)	1699
XmaJI	CCTAGG	1 cut(s)	242
XmiI	GTMKAC	1 cut(s)	1626
XmnI	GAANNNNTTC	1 cut(s)	1211
XspI	CTAG	6 cut(s)	243, 417, 840, 884, 1248, 1308
Zsp2I	ATGCAT	1 cut(s)	1103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.