Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001548

Physical Location & Seq

Genomic Coordinates Reverse (-)

30898708 .. 30903566

Gene Structure 4859 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1233100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGGCTGTGTGTTTAGCAGAGAAGGGTCTTCGGGAGTCGATTCTGAGCCAAAGGGGCAGAGGAGAGATTTGAGTGTAGAATCTGCTAGGAAGGTGGATGATGTTGTGGTGCAGAAGGTAGATAGTGAAAACGCCGAGGTTCATGTTGGTGGGGGTGAGGGCAAGAAGGAGGAAAATGGACATGGAGGTGAGAAACCTCCGAGGGGGGAGAGAAGGCGATCGAAGCCAAACCCCAGGTCAGGTAGTGTACCAAAGCAATCGCGTGGCGCCCAGGTGGCAGCAGGCTGGCCACCCTGGCTCACTGAGGCGTGTGGGGAGGCGATCAATGGGTGGACTCCACGGAAAGCAGACACTTTTGAGAAAATTGATAAGATTGGGTCCGGAACATATAGTAATGTGTACAAGGCCAAAGATATGTTGACAGGGAAAATTGTTGCCCTAAAGAAAGTTAGATTTGACAATTTGGAACCTGAGAGTGTGAGATTTATGGCTAGAGAAATTCTTATTTTGCGGAGACTGGATCATACCAATGTTGTAAAGTTGGAGGGTCTGGTTACATCGAGGATGTCATGTAGTTTGTACCTGGTGTTTGAATACATGGAGCATGATCTAGCTGGACTTGCCGCAAGCCCAGAAATTAAATTCACAGAATCTCAGGTAAAATGTTACATGCATCAACTACTATCTGGACTTGAGCACTGTCACAACCGTGGTGTGCTTCACCGTGACATTAAAGGATCAAATCTTTTGATTGACAATGGAGGAGTTCTTAAAATTGCCGACTTTGGATTGGCTTCTTTTTTTGATCCAAATCACAAGCATCCCATGACTAGTCGGGTGGTTACTCTATGGTATCGACCTCCCGAGCTTCTTCTTGGGGCTACTGATTACGGTGTGGGTGTGGACCTCTGGAGTGCAGGTTGCATTTTAGCAGAGTTACTAGCTGGGAAGCCTATCATGCCTGGTCGTACTGAGGTGGAGCAACTACATAAGATATACAAACTATGTGGTTCACCTTCAGATGAATACTGGAGGAAAGCAAAGTTGCCTAATGCAACTCTATTTAGGCCTCGGGAGCCTTACAGAAGATGCATAAGAGAGACATTCAAAGATTTTCCCCCATCATCATTGCCCCTTATTGAAACTCTTCTTGCAATCGATCCAGCAGAACGTCAGACAGCCACAGCTGCATTGAGCAGTGGGTTTTTTCTATCAGAGCCTTATGCTTGTGAACCTTCTAGCCTCCCAAAGTATCCCCCAACCAAAGAAATTGACGCCAAACGCCGGGACGACGAAGCTCGTAGACTGCGAGCTGCTGGGAGAGCCCAGGCTGATGGTCCAAAGAAAACACGCACACGGGAACGGGGTCCTAGGGGTATGCCAGCTCCAGAAGCCAATGCCGAGCTTCAGTCCAATATTGATAGAAGGCGCCTAATTACGCATGCAAATGCAAAGAGCAAAAGCGAAAAGTTTCCTCCACCGCACCAGGATGGGGCAGTTGGCTACCCTTTGGGATCTTCACATCATTTTGATCCCTCCCTTGTTCCTCCCGACGTTCCTTTCAGCACAACCTCATTCAGTTACCCTAAAGAGTCACTACAAGCTTGGTCAGGTCCGTTGGTCGACCCTGCTTCTGCCGGTGGTCCAAGACGGAAGAAACATACTGGACACGATGGGCGAGAAACTTCAAAATCCTTTACAGGAAACCATAGAGAGAGACATCACGATGCTCGTGTTAGAGGAAAGAGAAGTGTGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

587

Amino Acids

64.95

Weight (kDa)

9.38

Isoelectric Point (pI)

51.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	119 - 403	1.1e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	121 - 333	1.7e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001548)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54610 AT1G54610 AT1G54610 AT3G05050 AT3G05050 AT3G05050
fragaria_vesca	FvH4_4g17190 FvH4_4g17190 FvH4_4g17190 FvH4_4g17190 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100 FvH4_6g04100
malus_domestica	MD02G1048200.v1.1 MD04G1216500.v1.1 MD12G1233100.v1.1 MD13G1173900.v1.1 MD16G1175000.v1.1
prunus_persica	Prupe.1G142100_v2.0.a1 Prupe.1G142100_v2.0.a1 Prupe.6G336200_v2.0.a1 Prupe.6G336200_v2.0.a1
pyrus_communis	pycom04g19240 pycom12g21560
rosa_chinensis	RchiOBHm_Chr3g0452651 RchiOBHm_Chr4g0420991
rosa_laevigata	RLG00000007715 RLG00000025575
rosa_multiflora	Rmu_sc0006508.1_g000002 Rmu_ssc0000156.1_g000011
rosa_roxburghii	Rroxscaffold_5G00363340 Rroxscaffold_6G00429690
rosa_rugosa	Rorug02G0642200 Rorug02G0642300 Rorug02G0642300 Rorug02G0642400 Rorug04G0167500 Rorug04G0167500
rosa_samantha	Rh3AG044600 Rh3BG046900 Rh3CG045400 Rh3DG045800 Rh4AG229000 Rh4BG232000 Rh4CG243600 Rh4DG227400
rosa_wichuraiana	Rw3G003480 Rw4G019800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	908
AccB1I	GGYRCC	2 cut(s)	266, 1428
AccI	GTMKAC	2 cut(s)	1303, 1623
AccII	CGCG	1 cut(s)	262
AccIII	TCCGGA	1 cut(s)	380
AciI	CCGC	3 cut(s)	511, 624, 1481
AclWI	GGATC	6 cut(s)	528, 745, 800, 1154, 1522, 1526
AcoI	YGGCCR	1 cut(s)	287
AcsI	RAATTY	2 cut(s)	498, 641
AcuI	CTGAAG	2 cut(s)	1002, 1391
AcyI	GRCGYC	3 cut(s)	267, 1275, 1429
AfaI	GTAC	4 cut(s)	249, 401, 581, 970
AfiI	CCNNNNNNNGG	4 cut(s)	203, 239, 1284, 1492
AgsI	TTSAA	4 cut(s)	593, 1108, 1142, 1689
AhlI	ACTAGT	1 cut(s)	830
AjnI	CCWGG	7 cut(s)	233, 270, 293, 582, 961, 1326, 1485
AleI	CACNNNNGTG	1 cut(s)	708
AluBI	AGCT	9 cut(s)	614, 868, 944, 1187, 1298, 1313, 1385, 1405, 1604
AluI	AGCT	9 cut(s)	614, 868, 944, 1187, 1298, 1313, 1385, 1405, 1604
Alw21I	GWGCWC	1 cut(s)	699
Alw26I	GTCTC	3 cut(s)	508, 1094, 1711
AlwI	GGATC	6 cut(s)	528, 745, 800, 1154, 1522, 1526
Ama87I	CYCGRG	2 cut(s)	863, 1071
Aor13HI	TCCGGA	1 cut(s)	380
AoxI	GGCC	3 cut(s)	287, 405, 1067
ApeKI	GCWGC	3 cut(s)	278, 1187, 1313
ApoI	RAATTY	2 cut(s)	498, 641
ArsI	GACNNNNNNTTYG	2 cut(s)	221, 253
Asp700I	GAANNNNTTC	2 cut(s)	1146, 1470
AspA2I	CCTAGG	1 cut(s)	1370
AspLEI	GCGC	2 cut(s)	269, 1431
AspS9I	GGNCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
AsuC2I	CCSGG	1 cut(s)	1286
AsuHPI	GGTGA	4 cut(s)	167, 200, 713, 1005
AvaI	CYCGRG	2 cut(s)	863, 1071
AvaII	GGWCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
AvrII	CCTAGG	1 cut(s)	1370
BalI	TGGCCA	1 cut(s)	289
BanI	GGYRCC	2 cut(s)	266, 1428
BanII	GRGCYC	1 cut(s)	1327
BauI	CACGAG	1 cut(s)	1731
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	1 cut(s)	699
BbvI	GCAGC	3 cut(s)	290, 1174, 1300
BccI	CCATC	4 cut(s)	1129, 1328, 1484, 1667
BciT130I	CCWGG	7 cut(s)	235, 272, 295, 584, 963, 1328, 1487
BciVI	GTATCC	1 cut(s)	1263
BcnI	CCSGG	1 cut(s)	1286
BcoDI	GTCTC	3 cut(s)	508, 1094, 1711
BcuI	ACTAGT	1 cut(s)	830
BfaI	CTAG	7 cut(s)	87, 492, 611, 831, 941, 1239, 1371
BfoI	RGCGCY	2 cut(s)	270, 1432
BfuAI	ACCTGC	1 cut(s)	908
BfuI	GTATCC	1 cut(s)	1263
BglI	GCCNNNNNGGC	3 cut(s)	55, 275, 295
BisI	GCNGC	4 cut(s)	279, 624, 1188, 1314
BlnI	CCTAGG	1 cut(s)	1370
BlsI	GCNGC	4 cut(s)	280, 625, 1189, 1315
Bme1390I	CCNGG	8 cut(s)	235, 272, 295, 584, 963, 1286, 1328, 1487
Bme18I	GGWCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
BmeT110I	CYCGRG	2 cut(s)	863, 1071
BmgT120I	GGNCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
BmiI	GGNNCC	6 cut(s)	268, 379, 468, 1077, 1368, 1430
BmrFI	CCNGG	8 cut(s)	235, 272, 295, 584, 963, 1286, 1328, 1487
BmsI	GCATC	4 cut(s)	682, 829, 1079, 1717
BpiI	GAAGAC	1 cut(s)	21
BpmI	CTGGAG	3 cut(s)	931, 1051, 1371
BpuEI	CTTGAG	1 cut(s)	713
BpuMI	CCSGG	1 cut(s)	1286
Bsa29I	ATCGAT	1 cut(s)	1158
BsaBI	GATNNNNATC	1 cut(s)	810
BsaHI	GRCGYC	3 cut(s)	267, 1275, 1429
BsaWI	WCCGGW	1 cut(s)	380
BsaXI	ACNNNNNCTCC	2 cut(s)	904, 934
Bsc4I	CCNNNNNNNGG	4 cut(s)	203, 239, 1284, 1492
Bse118I	RCCGGY	1 cut(s)	1637
Bse1I	ACTGG	3 cut(s)	522, 1034, 1669
Bse3DI	GCAATG	1 cut(s)	1127
Bse8I	GATNNNNATC	1 cut(s)	810
BseAI	TCCGGA	1 cut(s)	380
BseBI	CCWGG	7 cut(s)	235, 272, 295, 584, 963, 1328, 1487
BseCI	ATCGAT	1 cut(s)	1158
BseGI	GGATG	4 cut(s)	103, 570, 820, 1495
BseJI	GATNNNNATC	1 cut(s)	810
BseLI	CCNNNNNNNGG	4 cut(s)	203, 239, 1284, 1492
BseMI	GCAATG	1 cut(s)	1127
BseMII	CTCAG	5 cut(s)	36, 294, 462, 668, 963
BseNI	ACTGG	3 cut(s)	522, 1034, 1669
BseRI	GAGGAG	2 cut(s)	76, 777
BseXI	GCAGC	3 cut(s)	290, 1174, 1300
BseYI	CCCAGC	2 cut(s)	944, 1316
BsgI	GTGCAG	2 cut(s)	131, 936
Bsh1236I	CGCG	1 cut(s)	262
Bsh1285I	CGRYCG	1 cut(s)	221
BshFI	GGCC	3 cut(s)	289, 407, 1069
BshNI	GGYRCC	2 cut(s)	266, 1428
BshVI	ATCGAT	1 cut(s)	1158
BsiEI	CGRYCG	1 cut(s)	221
BsiHKAI	GWGCWC	1 cut(s)	699
BsiHKCI	CYCGRG	2 cut(s)	863, 1071
BsiSI	CCGG	3 cut(s)	381, 1285, 1638
BslFI	GGGAC	1 cut(s)	1301
BslI	CCNNNNNNNGG	4 cut(s)	203, 239, 1284, 1492
BsmAI	GTCTC	3 cut(s)	508, 1094, 1711
BsmFI	GGGAC	1 cut(s)	1301
BsnI	GGCC	3 cut(s)	289, 407, 1069
BsoBI	CYCGRG	2 cut(s)	863, 1071
Bsp1286I	GDGCHC	2 cut(s)	699, 1327
Bsp13I	TCCGGA	1 cut(s)	380
Bsp1407I	TGTACA	1 cut(s)	399
Bsp143I	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
BspACI	CCGC	3 cut(s)	511, 624, 1481
BspANI	GGCC	3 cut(s)	289, 407, 1069
BspCNI	CTCAG	5 cut(s)	37, 295, 463, 667, 964
BspDI	ATCGAT	1 cut(s)	1158
BspEI	TCCGGA	1 cut(s)	380
BspFNI	CGCG	1 cut(s)	262
BspLI	GGNNCC	6 cut(s)	268, 379, 468, 1077, 1368, 1430
BspMI	ACCTGC	1 cut(s)	908
BspPI	GGATC	6 cut(s)	528, 745, 800, 1154, 1522, 1526
BspT107I	GGYRCC	2 cut(s)	266, 1428
BsrDI	GCAATG	1 cut(s)	1127
BsrFI	RCCGGY	1 cut(s)	1637
BsrGI	TGTACA	1 cut(s)	399
BsrI	ACTGG	3 cut(s)	522, 1034, 1669
BssAI	RCCGGY	1 cut(s)	1637
BssMI	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
BssNI	GRCGYC	3 cut(s)	267, 1275, 1429
BssSI	CACGAG	1 cut(s)	1731
BssT1I	CCWWGG	1 cut(s)	1370
Bst2BI	CACGAG	1 cut(s)	1731
Bst2UI	CCWGG	7 cut(s)	235, 272, 295, 584, 963, 1328, 1487
Bst4CI	ACNGT	4 cut(s)	701, 710, 725, 893
Bst6I	CTCTTC	1 cut(s)	1152
BstACI	GRCGYC	3 cut(s)	267, 1275, 1429
BstAUI	TGTACA	1 cut(s)	399
BstC8I	GCNNGC	6 cut(s)	283, 287, 628, 1311, 1383, 1443
BstDEI	CTNAG	5 cut(s)	45, 303, 471, 654, 972
BstDSI	CCRYGG	2 cut(s)	338, 709
BstF5I	GGATG	4 cut(s)	103, 570, 820, 1495
BstFNI	CGCG	1 cut(s)	262
BstH2I	RGCGCY	2 cut(s)	270, 1432
BstHHI	GCGC	2 cut(s)	269, 1431
BstKTI	GATC	9 cut(s)	221, 324, 523, 610, 740, 808, 1162, 1517, 1534
BstMAI	GTCTC	3 cut(s)	508, 1094, 1711
BstMBI	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
BstMCI	CGRYCG	1 cut(s)	221
BstNI	CCWGG	7 cut(s)	235, 272, 295, 584, 963, 1328, 1487
BstNSI	RCATGY	2 cut(s)	673, 1445
BstSCI	CCNGG	8 cut(s)	233, 270, 293, 582, 961, 1284, 1326, 1485
BstUI	CGCG	1 cut(s)	262
BstV1I	GCAGC	3 cut(s)	290, 1174, 1300
BstV2I	GAAGAC	1 cut(s)	21
BstX2I	RGATCY	1 cut(s)	1514
BstXI	CCANNNNNNTGG	1 cut(s)	1334
BstYI	RGATCY	1 cut(s)	1514
Bsu15I	ATCGAT	1 cut(s)	1158
BsuI	GTATCC	1 cut(s)	1263
BsuRI	GGCC	3 cut(s)	289, 407, 1069
BsuTUI	ATCGAT	1 cut(s)	1158
BtgI	CCRYGG	2 cut(s)	338, 709
BtsCI	GGATG	4 cut(s)	103, 570, 820, 1495
BtsI	GCAGTG	1 cut(s)	1204
BtsIMutI	CAGTG	3 cut(s)	300, 697, 1204
BveI	ACCTGC	1 cut(s)	908
Cac8I	GCNNGC	6 cut(s)	283, 287, 628, 1311, 1383, 1443
CfoI	GCGC	2 cut(s)	269, 1431
Cfr10I	RCCGGY	1 cut(s)	1637
Cfr13I	GGNCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
ClaI	ATCGAT	1 cut(s)	1158
CseI	GACGC	1 cut(s)	1283
CsiI	ACCWGGT	1 cut(s)	582
Csp6I	GTAC	4 cut(s)	248, 400, 580, 969
CspCI	CAANNNNNGTGG	2 cut(s)	988, 1023
CviAII	CATG	9 cut(s)	143, 182, 570, 598, 605, 670, 826, 958, 1442
CviQI	GTAC	4 cut(s)	248, 400, 580, 969
DdeI	CTNAG	5 cut(s)	45, 303, 471, 654, 972
DinI	GGCGCC	2 cut(s)	268, 1430
DpnI	GATC	9 cut(s)	220, 323, 522, 609, 739, 807, 1161, 1516, 1533
DpnII	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
EaeI	YGGCCR	1 cut(s)	287
Eam1104I	CTCTTC	1 cut(s)	1152
EarI	CTCTTC	1 cut(s)	1152
Eco130I	CCWWGG	1 cut(s)	1370
Eco147I	AGGCCT	1 cut(s)	1069
Eco24I	GRGCYC	1 cut(s)	1327
Eco47I	GGWCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
Eco57I	CTGAAG	2 cut(s)	1002, 1391
Eco88I	CYCGRG	2 cut(s)	863, 1071
EcoO109I	RGGNCCY	1 cut(s)	1367
EcoRII	CCWGG	7 cut(s)	233, 270, 293, 582, 961, 1326, 1485
EcoT14I	CCWWGG	1 cut(s)	1370
EcoT22I	ATGCAT	2 cut(s)	675, 1094
EcoT38I	GRGCYC	1 cut(s)	1327
EgeI	GGCGCC	2 cut(s)	268, 1430
EheI	GGCGCC	2 cut(s)	268, 1430
ErhI	CCWWGG	1 cut(s)	1370
FaeI	CATG	9 cut(s)	146, 185, 573, 601, 608, 673, 829, 961, 1445
FalI	AAGNNNNNCTT	1 cut(s)	1741
FaqI	GGGAC	1 cut(s)	1301
FatI	CATG	9 cut(s)	142, 181, 569, 597, 604, 669, 825, 957, 1441
FblI	GTMKAC	2 cut(s)	1303, 1623
Fnu4HI	GCNGC	4 cut(s)	279, 624, 1188, 1314
FokI	GGATG	4 cut(s)	110, 577, 807, 1502
FriOI	GRGCYC	1 cut(s)	1327
Fsp4HI	GCNGC	4 cut(s)	279, 624, 1188, 1314
FspBI	CTAG	7 cut(s)	87, 492, 611, 831, 941, 1239, 1371
GlaI	GCGC	2 cut(s)	268, 1430
GluI	GCNGC	4 cut(s)	279, 624, 1188, 1314
GsaI	CCCAGC	2 cut(s)	948, 1320
GsuI	CTGGAG	3 cut(s)	931, 1051, 1371
HaeII	RGCGCY	2 cut(s)	270, 1432
HaeIII	GGCC	3 cut(s)	289, 407, 1069
HapII	CCGG	3 cut(s)	381, 1285, 1638
HgaI	GACGC	1 cut(s)	1283
HhaI	GCGC	2 cut(s)	269, 1431
Hin1I	GRCGYC	3 cut(s)	267, 1275, 1429
Hin1II	CATG	9 cut(s)	146, 185, 573, 601, 608, 673, 829, 961, 1445
Hin6I	GCGC	2 cut(s)	267, 1429
HinP1I	GCGC	2 cut(s)	267, 1429
HincII	GTYRAC	2 cut(s)	420, 1624
HindII	GTYRAC	2 cut(s)	420, 1624
HindIII	AAGCTT	1 cut(s)	1602
HinfI	GANTC	6 cut(s)	36, 41, 80, 334, 650, 1592
HpaII	CCGG	3 cut(s)	381, 1285, 1638
HphI	GGTGA	4 cut(s)	167, 200, 713, 1005
Hpy166II	GTNNAC	9 cut(s)	248, 333, 400, 420, 904, 1013, 1232, 1304, 1624
Hpy188I	TCNGA	5 cut(s)	46, 201, 1021, 1176, 1216
Hpy188III	TCNNGA	9 cut(s)	33, 381, 687, 863, 910, 1073, 1388, 1550, 1724
Hpy8I	GTNNAC	9 cut(s)	248, 333, 400, 420, 904, 1013, 1232, 1304, 1624
Hpy99I	CGWCG	2 cut(s)	1295, 1556
HpyAV	CCTTC	8 cut(s)	17, 85, 109, 160, 207, 1026, 1245, 1419
HpyCH4III	ACNGT	4 cut(s)	701, 710, 725, 893
HpyCH4IV	ACGT	2 cut(s)	1171, 1554
HpyF3I	CTNAG	5 cut(s)	45, 303, 471, 654, 972
HpySE526I	ACGT	2 cut(s)	1171, 1554
Hsp92I	GRCGYC	3 cut(s)	267, 1275, 1429
Hsp92II	CATG	9 cut(s)	146, 185, 573, 601, 608, 673, 829, 961, 1445
HspAI	GCGC	2 cut(s)	267, 1429
KasI	GGCGCC	2 cut(s)	266, 1428
Kpn2I	TCCGGA	1 cut(s)	380
Kzo9I	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
LmnI	GCTCC	4 cut(s)	601, 979, 1075, 1390
Lsp1109I	GCAGC	3 cut(s)	290, 1174, 1300
LweI	GCATC	4 cut(s)	682, 829, 1079, 1717
MabI	ACCWGGT	1 cut(s)	582
MaeI	CTAG	7 cut(s)	87, 492, 611, 831, 941, 1239, 1371
MaeII	ACGT	2 cut(s)	1171, 1554
MaeIII	GTNAC	8 cut(s)	553, 665, 701, 725, 841, 936, 1580, 1593
MalI	GATC	9 cut(s)	220, 323, 522, 609, 739, 807, 1161, 1516, 1533
MboI	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
MboII	GAAGA	6 cut(s)	21, 863, 1098, 1139, 1509, 1666
MflI	RGATCY	1 cut(s)	1514
MhlI	GDGCHC	2 cut(s)	699, 1327
MlsI	TGGCCA	1 cut(s)	289
MluCI	AATT	9 cut(s)	363, 429, 460, 498, 636, 641, 774, 1269, 1434
MluNI	TGGCCA	1 cut(s)	289
Mly113I	GGCGCC	2 cut(s)	267, 1429
MlyI	GAGTC	3 cut(s)	45, 328, 1601
MmeI	TCCRAC	1 cut(s)	522
Mox20I	TGGCCA	1 cut(s)	289
Mph1103I	ATGCAT	2 cut(s)	675, 1094
MroI	TCCGGA	1 cut(s)	380
MroXI	GAANNNNTTC	2 cut(s)	1146, 1470
MscI	TGGCCA	1 cut(s)	289
MseI	TTAA	3 cut(s)	639, 732, 771
MslI	CAYNNNNRTG	7 cut(s)	147, 186, 528, 708, 1446, 1488, 1725
Msp20I	TGGCCA	1 cut(s)	289
MspA1I	CMGCKG	1 cut(s)	1187
MspI	CCGG	3 cut(s)	381, 1285, 1638
MspR9I	CCNGG	8 cut(s)	235, 272, 295, 584, 963, 1286, 1328, 1487
MvaI	CCWGG	7 cut(s)	235, 272, 295, 584, 963, 1328, 1487
MvnI	CGCG	1 cut(s)	262
NarI	GGCGCC	2 cut(s)	267, 1429
NciI	CCSGG	1 cut(s)	1286
NdeII	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
NlaIII	CATG	9 cut(s)	146, 185, 573, 601, 608, 673, 829, 961, 1445
NlaIV	GGNNCC	6 cut(s)	268, 379, 468, 1077, 1368, 1430
NmeAIII	GCCGAG	2 cut(s)	160, 1426
NmuCI	GTSAC	3 cut(s)	701, 725, 1593
NsiI	ATGCAT	2 cut(s)	675, 1094
NspI	RCATGY	2 cut(s)	673, 1445
OliI	CACNNNNGTG	1 cut(s)	708
PaeI	GCATGC	1 cut(s)	1445
PceI	AGGCCT	1 cut(s)	1069
PcsI	WCGNNNNNNNCGW	1 cut(s)	1306
PdmI	GAANNNNTTC	2 cut(s)	1146, 1470
PfeI	GAWTC	3 cut(s)	41, 80, 650
PkrI	GCNGC	4 cut(s)	280, 625, 1189, 1315
Ple19I	CGATCG	1 cut(s)	221
PleI	GAGTC	3 cut(s)	44, 328, 1600
PluTI	GGCGCC	2 cut(s)	270, 1432
PpsI	GAGTC	3 cut(s)	44, 328, 1600
PpuMI	RGGWCCY	1 cut(s)	1367
Psp5II	RGGWCCY	1 cut(s)	1367
Psp6I	CCWGG	7 cut(s)	233, 270, 293, 582, 961, 1326, 1485
PspFI	CCCAGC	2 cut(s)	944, 1316
PspGI	CCWGG	7 cut(s)	233, 270, 293, 582, 961, 1326, 1485
PspN4I	GGNNCC	6 cut(s)	268, 379, 468, 1077, 1368, 1430
PspPI	GGNCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
PspPPI	RGGWCCY	1 cut(s)	1367
PsuI	RGATCY	1 cut(s)	1514
PvuI	CGATCG	1 cut(s)	221
PvuII	CAGCTG	1 cut(s)	1187
RsaI	GTAC	4 cut(s)	249, 401, 581, 970
RsaNI	GTAC	4 cut(s)	248, 400, 580, 969
RseI	CAYNNNNRTG	7 cut(s)	147, 186, 528, 708, 1446, 1488, 1725
SalI	GTCGAC	1 cut(s)	1622
SaqAI	TTAA	3 cut(s)	639, 732, 771
SatI	GCNGC	4 cut(s)	279, 624, 1188, 1314
Sau3AI	GATC	9 cut(s)	218, 321, 520, 607, 737, 805, 1159, 1514, 1531
Sau96I	GGNCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
SchI	GAGTC	3 cut(s)	45, 328, 1601
ScrFI	CCNGG	8 cut(s)	235, 272, 295, 584, 963, 1286, 1328, 1487
SduI	GDGCHC	2 cut(s)	699, 1327
SexAI	ACCWGGT	1 cut(s)	582
SfaNI	GCATC	4 cut(s)	682, 829, 1079, 1717
SfoI	GGCGCC	2 cut(s)	268, 1430
SinI	GGWCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
SmiMI	CAYNNNNRTG	7 cut(s)	147, 186, 528, 708, 1446, 1488, 1725
SmlI	CTYRAG	1 cut(s)	692
SmoI	CTYRAG	1 cut(s)	692
SpeI	ACTAGT	1 cut(s)	830
SphI	GCATGC	1 cut(s)	1445
Sse9I	AATT	9 cut(s)	363, 429, 460, 498, 636, 641, 774, 1269, 1434
SseBI	AGGCCT	1 cut(s)	1069
SsiI	CCGC	3 cut(s)	511, 624, 1481
SspDI	GGCGCC	2 cut(s)	266, 1428
SspI	AATATT	1 cut(s)	1417
SspMI	CTAG	7 cut(s)	87, 492, 611, 831, 941, 1239, 1371
StuI	AGGCCT	1 cut(s)	1069
StyD4I	CCNGG	8 cut(s)	233, 270, 293, 582, 961, 1284, 1326, 1485
StyI	CCWWGG	1 cut(s)	1370
TaaI	ACNGT	4 cut(s)	701, 710, 725, 893
TaiI	ACGT	2 cut(s)	1174, 1557
TaqI	TCGA	6 cut(s)	39, 221, 560, 856, 1158, 1623
TasI	AATT	9 cut(s)	363, 429, 460, 498, 636, 641, 774, 1269, 1434
TatI	WGTACW	1 cut(s)	399
TauI	GCSGC	1 cut(s)	626
TfiI	GAWTC	3 cut(s)	41, 80, 650
Tru1I	TTAA	3 cut(s)	639, 732, 771
Tru9I	TTAA	3 cut(s)	639, 732, 771
TscAI	CASTG	3 cut(s)	307, 704, 1204
TseFI	GTSAC	3 cut(s)	701, 725, 1593
TseI	GCWGC	3 cut(s)	278, 1187, 1313
Tsp45I	GTSAC	3 cut(s)	701, 725, 1593
TspDTI	ATGAA	2 cut(s)	131, 1038
TspGWI	ACGGA	3 cut(s)	355, 1605, 1666
TspRI	CASTG	3 cut(s)	307, 704, 1204
VpaK11BI	GGWCC	6 cut(s)	378, 904, 1337, 1367, 1613, 1643
XapI	RAATTY	2 cut(s)	498, 641
XceI	RCATGY	2 cut(s)	673, 1445
XmaJI	CCTAGG	1 cut(s)	1370
XmiI	GTMKAC	2 cut(s)	1303, 1623
XmnI	GAANNNNTTC	2 cut(s)	1146, 1470
XspI	CTAG	7 cut(s)	87, 492, 611, 831, 941, 1239, 1371
Zsp2I	ATGCAT	2 cut(s)	675, 1094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.