MD04G1229900.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006115
Physical Location & Seq
Forward (+)
31014810 .. 31017986
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1229900.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGCGAACCCACCGTCTTCTTCCACAGCTCCTCCGTCGACCTCTTCCCAATTCACATATGCCAACGCCTCGTCGTCGTCGTCCTACTTCCCCATGCCATTCCACCTCCAGCAACCCCAGTACCCCGCTCCTTACGCCGTCGCAGCGCCACCCGCCCCATCCGTCTACCCGGCCCCGGCTCCGGTCGCTGGTGTCTACTCTCTCCCTCAGCAGTATCAGCAGGCTCAGCAGTTGTTTCAAAGGGATGCGCAGACGATTACACCTGAGGCTCTTGAGAGTGTGAAAGCTGCTCTTGCAAACAGCGAAATTGAGCACAAAGCGGAAGCTAAGAAGAAAGCTATCCCTCGTAAAGCTGCCGGGCAGGCCTGGGAGGACCCAACTCTTGCAGAATGGCCAGAAAATGATTATCGACTTTTTTGTGGTGATCTTGGTAATGAGGTGAATGATGATGTTCTTTCAAAAGCATTTTCACGATTTCCTTCCTTTAACATGGCAAGAGTTGTCAGAGATAAGAGGACTGGAAAAACCAAGGGCTTTGGATTTGTTAGCTTTGCCAACCCTTCTGACCTGGCCGGAGCACTAAAGGAAATGAATGGTAAGTACGTGGGCAATCGGCCAATCAAACTACGGAAAAGTAACTGGAGGGAGAGGATAGATTATGATGCACTAGAAAGACAAAAGCACCACACTCACAAGAAACCAAAGCCTTCGAAGAAGAGTATATTGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

245

Amino Acids

27.06

Weight (kDa)

9.62

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 138 - 208 1e-18 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 249
AccBSI CCGCTC 1 cut(s) 128
AccI GTMKAC 3 cut(s) 38, 165, 195
AciI CCGC 3 cut(s) 126, 153, 320
AcoI YGGCCR 3 cut(s) 392, 570, 614
AfaI GTAC 2 cut(s) 122, 602
AfiI CCNNNNNNNGG 3 cut(s) 175, 181, 188
AgsI TTSAA 2 cut(s) 239, 459
AjnI CCWGG 2 cut(s) 365, 567
AluBI AGCT 6 cut(s) 29, 287, 326, 338, 353, 549
AluI AGCT 6 cut(s) 29, 287, 326, 338, 353, 549
Alw21I GWGCWC 2 cut(s) 315, 580
AoxI GGCC 5 cut(s) 171, 363, 392, 570, 614
ApeKI GCWGC 3 cut(s) 143, 287, 353
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 2 cut(s) 148, 250
AspS9I GGNCC 2 cut(s) 172, 373
AsuC2I CCSGG 3 cut(s) 170, 176, 358
AsuHPI GGTGA 2 cut(s) 434, 451
AsuII TTCGAA 1 cut(s) 710
AvaII GGWCC 1 cut(s) 373
AxyI CCTNAGG 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 104, 137
BalI TGGCCA 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 315, 580
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 3 cut(s) 155, 274, 340
BccI CCATC 1 cut(s) 166
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 2 cut(s) 367, 569
BcnI CCSGG 3 cut(s) 170, 176, 358
BfaI CTAG 1 cut(s) 668
BfoI RGCGCY 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 3 cut(s) 144, 288, 354
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 3 cut(s) 145, 289, 355
Bme1390I CCNGG 5 cut(s) 170, 176, 358, 367, 569
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 172, 373
BmiI GGNNCC 3 cut(s) 174, 180, 375
BmrFI CCNGG 5 cut(s) 170, 176, 358, 367, 569
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 2 cut(s) 235, 652
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 92, 661
Bpu10I CCTNAGC 1 cut(s) 207
Bpu1102I GCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 3 cut(s) 170, 176, 358
BsaAI YACGTR 1 cut(s) 604
BsaJI CCNNGG 3 cut(s) 174, 366, 528
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 181, 188
Bse1I ACTGG 3 cut(s) 118, 523, 644
Bse21I CCTNAGG 1 cut(s) 264
BseBI CCWGG 2 cut(s) 367, 569
BseDI CCNNGG 3 cut(s) 174, 366, 528
BseGI GGATG 2 cut(s) 158, 250
BseLI CCNNNNNNNGG 3 cut(s) 175, 181, 188
BseMII CTCAG 3 cut(s) 221, 239, 255
BseNI ACTGG 3 cut(s) 118, 523, 644
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 3 cut(s) 155, 274, 340
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 5 cut(s) 173, 365, 394, 572, 616
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 315, 580
BsiSI CCGG 5 cut(s) 170, 176, 182, 357, 573
BslI CCNNNNNNNGG 3 cut(s) 175, 181, 188
BsnI GGCC 5 cut(s) 173, 365, 394, 572, 616
Bsp119I TTCGAA 1 cut(s) 710
Bsp1286I GDGCHC 2 cut(s) 315, 580
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 3 cut(s) 126, 153, 320
BspANI GGCC 5 cut(s) 173, 365, 394, 572, 616
BspCNI CTCAG 3 cut(s) 220, 238, 256
BspLI GGNNCC 3 cut(s) 174, 180, 375
BspT104I TTCGAA 1 cut(s) 710
BsrBI CCGCTC 1 cut(s) 128
BsrI ACTGG 3 cut(s) 118, 523, 644
BssECI CCNNGG 3 cut(s) 174, 366, 528
BssMI GATC 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 2 cut(s) 367, 569
Bst4CI ACNGT 1 cut(s) 15
Bst6I CTCTTC 2 cut(s) 49, 710
BstBAI YACGTR 1 cut(s) 604
BstBI TTCGAA 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 222, 363
BstDEI CTNAG 4 cut(s) 207, 225, 264, 327
BstF5I GGATG 2 cut(s) 158, 250
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 2 cut(s) 148, 250
BstKTI GATC 1 cut(s) 427
BstMBI GATC 1 cut(s) 424
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
BstNI CCWGG 2 cut(s) 367, 569
BstSCI CCNGG 5 cut(s) 168, 174, 356, 365, 567
BstV1I GCAGC 3 cut(s) 155, 274, 340
BstV2I GAAGAC 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 5 cut(s) 173, 365, 394, 572, 616
BtsCI GGATG 2 cut(s) 158, 250
Cac8I GCNNGC 2 cut(s) 222, 363
CfoI GCGC 2 cut(s) 148, 250
Cfr13I GGNCC 2 cut(s) 172, 373
Csp6I GTAC 2 cut(s) 121, 601
CviAII CATG 2 cut(s) 94, 490
CviQI GTAC 2 cut(s) 121, 601
DdeI CTNAG 4 cut(s) 207, 225, 264, 327
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 3 cut(s) 392, 570, 614
Eam1104I CTCTTC 2 cut(s) 49, 710
EarI CTCTTC 2 cut(s) 49, 710
Eco130I CCWWGG 1 cut(s) 528
Eco147I AGGCCT 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 373
Eco81I CCTNAGG 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 2 cut(s) 365, 567
EcoT14I CCWWGG 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 2 cut(s) 97, 493
FaiI YATR 6 cut(s) 58, 60, 95, 491, 660, 722
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FatI CATG 2 cut(s) 93, 489
FauI CCCGC 2 cut(s) 133, 160
FauNDI CATATG 1 cut(s) 58
FblI GTMKAC 3 cut(s) 38, 165, 195
Fnu4HI GCNGC 3 cut(s) 144, 288, 354
FokI GGATG 2 cut(s) 145, 257
Fsp4HI GCNGC 3 cut(s) 144, 288, 354
FspBI CTAG 1 cut(s) 668
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 2 cut(s) 147, 249
GluI GCNGC 3 cut(s) 144, 288, 354
GsuI CTGGAG 2 cut(s) 92, 661
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 5 cut(s) 173, 365, 394, 572, 616
HapII CCGG 5 cut(s) 170, 176, 182, 357, 573
HhaI GCGC 2 cut(s) 148, 250
Hin1II CATG 2 cut(s) 97, 493
Hin6I GCGC 2 cut(s) 146, 248
HinP1I GCGC 2 cut(s) 146, 248
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HpaII CCGG 5 cut(s) 170, 176, 182, 357, 573
HphI GGTGA 2 cut(s) 434, 451
Hpy166II GTNNAC 3 cut(s) 39, 166, 196
Hpy188I TCNGA 2 cut(s) 506, 565
Hpy188III TCNNGA 2 cut(s) 272, 471
Hpy8I GTNNAC 3 cut(s) 39, 166, 196
Hpy99I CGWCG 5 cut(s) 40, 76, 79, 82, 143
HpyAV CCTTC 3 cut(s) 489, 570, 717
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 4 cut(s) 296, 386, 665, 727
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
HpyF3I CTNAG 4 cut(s) 207, 225, 264, 327
HpySE526I ACGT 1 cut(s) 603
Hsp92II CATG 2 cut(s) 97, 493
HspAI GCGC 2 cut(s) 146, 248
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 4 cut(s) 34, 133, 184, 575
Lsp1109I GCAGC 3 cut(s) 155, 274, 340
LweI GCATC 2 cut(s) 235, 652
MaeI CTAG 1 cut(s) 668
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 1 cut(s) 635
MalI GATC 1 cut(s) 426
MbiI CCGCTC 1 cut(s) 128
MboI GATC 1 cut(s) 424
MboII GAAGA 6 cut(s) 9, 12, 36, 343, 724, 727
MhlI GDGCHC 2 cut(s) 315, 580
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 2 cut(s) 50, 306
MluNI TGGCCA 1 cut(s) 394
Mox20I TGGCCA 1 cut(s) 394
MscI TGGCCA 1 cut(s) 394
MseI TTAA 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 394
MspI CCGG 5 cut(s) 170, 176, 182, 357, 573
MspR9I CCNGG 5 cut(s) 170, 176, 358, 367, 569
MvaI CCWGG 2 cut(s) 367, 569
MwoI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
NciI CCSGG 3 cut(s) 170, 176, 358
NdeI CATATG 1 cut(s) 58
NdeII GATC 1 cut(s) 424
NlaIII CATG 2 cut(s) 97, 493
NlaIV GGNNCC 3 cut(s) 174, 180, 375
NsbI TGCGCA 1 cut(s) 249
NspV TTCGAA 1 cut(s) 710
PceI AGGCCT 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PkrI GCNGC 3 cut(s) 145, 289, 355
Ppu21I YACGTR 1 cut(s) 604
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 365, 567
PspGI CCWGG 2 cut(s) 365, 567
PspN4I GGNNCC 3 cut(s) 174, 180, 375
PspPI GGNCC 2 cut(s) 172, 373
PspPPI RGGWCCY 1 cut(s) 373
RsaI GTAC 2 cut(s) 122, 602
RsaNI GTAC 2 cut(s) 121, 601
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 3 cut(s) 144, 288, 354
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 2 cut(s) 172, 373
ScrFI CCNGG 5 cut(s) 170, 176, 358, 367, 569
SduI GDGCHC 2 cut(s) 315, 580
SfaNI GCATC 2 cut(s) 235, 652
SfuI TTCGAA 1 cut(s) 710
SinI GGWCC 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 50, 306
SseBI AGGCCT 1 cut(s) 365
SsiI CCGC 3 cut(s) 126, 153, 320
SspMI CTAG 1 cut(s) 668
StuI AGGCCT 1 cut(s) 365
StyD4I CCNGG 5 cut(s) 168, 174, 356, 365, 567
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 15
TaiI ACGT 1 cut(s) 606
TaqI TCGA 3 cut(s) 38, 409, 710
TasI AATT 2 cut(s) 50, 306
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseI GCWGC 3 cut(s) 143, 287, 353
TspDTI ATGAA 1 cut(s) 605
TspGWI ACGGA 3 cut(s) 24, 151, 643
VpaK11BI GGWCC 1 cut(s) 373
XmiI GTMKAC 3 cut(s) 38, 165, 195
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.