MD12G1247300.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006115
Physical Location & Seq
Forward (+)
31775624 .. 31778498
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1247300.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGCGAACCCACCGTCTTCCTCCGCAGCTCCTCCATTGACCTCTTCCCAATTCACATACGCGAATGCCTCGTCGTCATCGTCCTACTTCCCCATGCCCTTCCACCTCCAGCAACCCCAGTACCCCGCGCCTTACGCCGTTGCAGCGCCACCCGCTCCGTCCGTCTACCCGGCCCCGGCTCCGGTCGCCGGTGTCTACTCTCTTCCTCAGCAGTATCAGCAGGCTCAGCAGTTGTTTCAAAGGGATGCGCAGACGATTACACCTGAGGCTCTCGAGAGTGTGAAGGCTGCTCTTGCAAACAGCGAAATTGAGCACAAAGCAGAAGCTAAGAAGAAAGCTATCCCTCGTAAAGCTGCCGGGCAGGCTTGGGAGGACCCAACTCTTGCAGAATGGCCAGAAAATGATTATCGACTTTTTTGTGGCGATCTTGGTAATGAGGTGAATGATGATGTTCTTTCAAAAGCATTTTCACGATTTCCATCCTTTAACATGGCAAGAGTTGTCAGAGATAAGAGGACTGGAAAAACCAAGGGCTTTGGATTTGTTAGCTTTGCCAACCCATCTGACCTGGCCGGAGCACTAAAGGAAATGAATGGTAAGTATGTGGGCAATCGGCCAATCAAACTACGCAAAAGTAACTGGAGGGAGAGGATAGATTATGACGCACTAGAAAGACAAAAGGTACCATTTCCACTTGTTTACTGTAGTTTCAAATTTACGGCTTTCCAAAAGGTAATCTGTACTTACCAAGTGTTCCTGCTTTATTTCATTTGTGGCAGAACCACACTCACAAGAAACCAAAGCCTTCGAAGAAGAGTATATTGCACAAGTGAGCTAGAGACTAGTGGGCGACGCGGTGAATATGGAAATTGGGGCAGTGAAGAAGTAAGTGTTCTTTTTATTCCTCAAATCCAACTCGGCTCCTGGTGTTTACTGCTATGTAGAGATATGGTGAAGCTTAATGATGATTATTCGAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

328

Amino Acids

36.74

Weight (kDa)

9.11

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 138 - 208 1.7e-18 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 249
Acc65I GGTACC 1 cut(s) 682
AccB1I GGYRCC 1 cut(s) 682
AccBSI CCGCTC 1 cut(s) 155
AccI GTMKAC 2 cut(s) 165, 195
AccII CGCG 3 cut(s) 62, 128, 855
AciI CCGC 4 cut(s) 24, 126, 153, 855
AcoI YGGCCR 3 cut(s) 392, 570, 614
AcsI RAATTY 1 cut(s) 713
AfaI GTAC 3 cut(s) 122, 684, 742
AfiI CCNNNNNNNGG 3 cut(s) 175, 181, 188
AgsI TTSAA 3 cut(s) 239, 459, 712
AhlI ACTAGT 1 cut(s) 842
AjnI CCWGG 2 cut(s) 567, 923
AluBI AGCT 7 cut(s) 29, 326, 338, 353, 549, 835, 958
AluI AGCT 7 cut(s) 29, 326, 338, 353, 549, 835, 958
Alw21I GWGCWC 2 cut(s) 315, 580
Alw26I GTCTC 1 cut(s) 833
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 4 cut(s) 171, 392, 570, 614
ApeKI GCWGC 4 cut(s) 26, 143, 287, 353
ApoI RAATTY 1 cut(s) 713
ArsI GACNNNNNNTTYG 3 cut(s) 31, 56, 88
Asp718I GGTACC 1 cut(s) 682
AspLEI GCGC 3 cut(s) 130, 148, 250
AspS9I GGNCC 2 cut(s) 172, 373
AsuC2I CCSGG 3 cut(s) 170, 176, 358
AsuHPI GGTGA 3 cut(s) 451, 869, 964
AsuII TTCGAA 2 cut(s) 808, 974
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 373
AxyI CCTNAGG 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 104, 137
BalI TGGCCA 1 cut(s) 394
BanI GGYRCC 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 315, 580
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 38, 155, 274, 340
BccI CCATC 2 cut(s) 487, 568
BceAI ACGGC 2 cut(s) 122, 735
BciT130I CCWGG 2 cut(s) 569, 925
BcnI CCSGG 3 cut(s) 170, 176, 358
BcoDI GTCTC 1 cut(s) 833
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 3 cut(s) 668, 836, 843
BfmI CTRYAG 1 cut(s) 703
BfoI RGCGCY 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 4 cut(s) 27, 144, 288, 354
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 4 cut(s) 28, 145, 289, 355
Bme1390I CCNGG 5 cut(s) 170, 176, 358, 569, 925
Bme18I GGWCC 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 172, 373
BmiI GGNNCC 5 cut(s) 174, 180, 375, 684, 922
BmrFI CCNGG 5 cut(s) 170, 176, 358, 569, 925
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 92, 661
Bpu10I CCTNAGC 1 cut(s) 207
Bpu1102I GCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 2 cut(s) 808, 974
BpuMI CCSGG 3 cut(s) 170, 176, 358
BsaBI GATNNNNATC 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 174, 528
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 181, 188
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 3 cut(s) 118, 523, 644
Bse21I CCTNAGG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 478
BseBI CCWGG 2 cut(s) 569, 925
BseDI CCNNGG 2 cut(s) 174, 528
BseGI GGATG 2 cut(s) 250, 479
BseJI GATNNNNATC 1 cut(s) 478
BseLI CCNNNNNNNGG 3 cut(s) 175, 181, 188
BseMII CTCAG 3 cut(s) 221, 239, 255
BseNI ACTGG 3 cut(s) 118, 523, 644
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 4 cut(s) 38, 155, 274, 340
Bsh1236I CGCG 3 cut(s) 62, 128, 855
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 4 cut(s) 173, 394, 572, 616
BshNI GGYRCC 1 cut(s) 682
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 315, 580
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 6 cut(s) 170, 176, 182, 189, 357, 573
BslI CCNNNNNNNGG 3 cut(s) 175, 181, 188
BsmAI GTCTC 1 cut(s) 833
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 4 cut(s) 173, 394, 572, 616
BsoBI CYCGRG 1 cut(s) 272
Bsp119I TTCGAA 2 cut(s) 808, 974
Bsp1286I GDGCHC 2 cut(s) 315, 580
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 4 cut(s) 24, 126, 153, 855
BspANI GGCC 4 cut(s) 173, 394, 572, 616
BspCNI CTCAG 3 cut(s) 220, 238, 256
BspFNI CGCG 3 cut(s) 62, 128, 855
BspLI GGNNCC 5 cut(s) 174, 180, 375, 684, 922
BspT104I TTCGAA 2 cut(s) 808, 974
BspT107I GGYRCC 1 cut(s) 682
BsrBI CCGCTC 1 cut(s) 155
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 3 cut(s) 118, 523, 644
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 2 cut(s) 174, 528
BssMI GATC 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 2 cut(s) 569, 925
Bst4CI ACNGT 2 cut(s) 15, 704
Bst6I CTCTTC 3 cut(s) 49, 207, 808
BstBI TTCGAA 2 cut(s) 808, 974
BstC8I GCNNGC 2 cut(s) 222, 363
BstDEI CTNAG 4 cut(s) 207, 225, 264, 327
BstF5I GGATG 2 cut(s) 250, 479
BstFNI CGCG 3 cut(s) 62, 128, 855
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 130, 148, 250
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 1 cut(s) 424
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
BstNI CCWGG 2 cut(s) 569, 925
BstSCI CCNGG 5 cut(s) 168, 174, 356, 567, 923
BstSFI CTRYAG 1 cut(s) 703
BstUI CGCG 3 cut(s) 62, 128, 855
BstV1I GCAGC 4 cut(s) 38, 155, 274, 340
BstV2I GAAGAC 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 4 cut(s) 173, 394, 572, 616
BtsCI GGATG 2 cut(s) 250, 479
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 1 cut(s) 883
Cac8I GCNNGC 2 cut(s) 222, 363
CfoI GCGC 3 cut(s) 130, 148, 250
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 172, 373
CseI GACGC 2 cut(s) 671, 861
Csp6I GTAC 3 cut(s) 121, 683, 741
CviAII CATG 2 cut(s) 94, 490
CviQI GTAC 3 cut(s) 121, 683, 741
DdeI CTNAG 4 cut(s) 207, 225, 264, 327
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 3 cut(s) 392, 570, 614
Eam1104I CTCTTC 3 cut(s) 49, 207, 808
EarI CTCTTC 3 cut(s) 49, 207, 808
Eco130I CCWWGG 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 373
Eco81I CCTNAGG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 2 cut(s) 567, 923
EcoT14I CCWWGG 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 2 cut(s) 97, 493
FaiI YATR 9 cut(s) 58, 95, 491, 603, 660, 820, 864, 940, 950
FatI CATG 2 cut(s) 93, 489
FauI CCCGC 2 cut(s) 133, 160
FblI GTMKAC 2 cut(s) 165, 195
Fnu4HI GCNGC 4 cut(s) 27, 144, 288, 354
FokI GGATG 2 cut(s) 257, 466
Fsp4HI GCNGC 4 cut(s) 27, 144, 288, 354
FspBI CTAG 3 cut(s) 668, 836, 843
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 3 cut(s) 129, 147, 249
GluI GCNGC 4 cut(s) 27, 144, 288, 354
GsuI CTGGAG 2 cut(s) 92, 661
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 4 cut(s) 173, 394, 572, 616
HapII CCGG 6 cut(s) 170, 176, 182, 189, 357, 573
HgaI GACGC 2 cut(s) 671, 861
HhaI GCGC 3 cut(s) 130, 148, 250
Hin1II CATG 2 cut(s) 97, 493
Hin6I GCGC 3 cut(s) 128, 146, 248
HinP1I GCGC 3 cut(s) 128, 146, 248
HindIII AAGCTT 1 cut(s) 956
HpaII CCGG 6 cut(s) 170, 176, 182, 189, 357, 573
HphI GGTGA 3 cut(s) 451, 869, 964
Hpy166II GTNNAC 4 cut(s) 166, 196, 700, 932
Hpy188I TCNGA 2 cut(s) 506, 565
Hpy188III TCNNGA 3 cut(s) 272, 274, 471
Hpy8I GTNNAC 4 cut(s) 166, 196, 700, 932
Hpy99I CGWCG 2 cut(s) 76, 855
HpyAV CCTTC 3 cut(s) 109, 277, 815
HpyCH4III ACNGT 2 cut(s) 15, 704
HpyCH4V TGCA 4 cut(s) 143, 296, 386, 825
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
HpyF3I CTNAG 4 cut(s) 207, 225, 264, 327
Hsp92II CATG 2 cut(s) 97, 493
HspAI GCGC 3 cut(s) 128, 146, 248
KpnI GGTACC 1 cut(s) 686
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 5 cut(s) 34, 160, 184, 575, 926
Lsp1109I GCAGC 4 cut(s) 38, 155, 274, 340
LweI GCATC 1 cut(s) 235
MaeI CTAG 3 cut(s) 668, 836, 843
MaeIII GTNAC 1 cut(s) 635
MalI GATC 1 cut(s) 426
MbiI CCGCTC 1 cut(s) 155
MboI GATC 1 cut(s) 424
MboII GAAGA 7 cut(s) 9, 36, 194, 343, 822, 825, 893
MhlI GDGCHC 2 cut(s) 315, 580
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 4 cut(s) 50, 306, 713, 868
MluNI TGGCCA 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 937
Mox20I TGGCCA 1 cut(s) 394
MscI TGGCCA 1 cut(s) 394
MseI TTAA 2 cut(s) 486, 960
Msp20I TGGCCA 1 cut(s) 394
MspI CCGG 6 cut(s) 170, 176, 182, 189, 357, 573
MspR9I CCNGG 5 cut(s) 170, 176, 358, 569, 925
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 2 cut(s) 569, 925
MvnI CGCG 3 cut(s) 62, 128, 855
MwoI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
NciI CCSGG 3 cut(s) 170, 176, 358
NdeII GATC 1 cut(s) 424
NlaIII CATG 2 cut(s) 97, 493
NlaIV GGNNCC 5 cut(s) 174, 180, 375, 684, 922
NmeAIII GCCGAG 1 cut(s) 897
NsbI TGCGCA 1 cut(s) 249
NspV TTCGAA 2 cut(s) 808, 974
PaeR7I CTCGAG 1 cut(s) 272
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PctI GAATGC 1 cut(s) 70
PkrI GCNGC 4 cut(s) 28, 145, 289, 355
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 567, 923
PspGI CCWGG 2 cut(s) 567, 923
PspN4I GGNNCC 5 cut(s) 174, 180, 375, 684, 922
PspPI GGNCC 2 cut(s) 172, 373
PspPPI RGGWCCY 1 cut(s) 373
RsaI GTAC 3 cut(s) 122, 684, 742
RsaNI GTAC 3 cut(s) 121, 683, 741
SaqAI TTAA 2 cut(s) 486, 960
SatI GCNGC 4 cut(s) 27, 144, 288, 354
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 2 cut(s) 172, 373
ScrFI CCNGG 5 cut(s) 170, 176, 358, 569, 925
SduI GDGCHC 2 cut(s) 315, 580
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 703
Sfr274I CTCGAG 1 cut(s) 272
SfuI TTCGAA 2 cut(s) 808, 974
SgrAI CRCCGGYG 1 cut(s) 188
SinI GGWCC 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SpeI ACTAGT 1 cut(s) 842
Sse9I AATT 4 cut(s) 50, 306, 713, 868
SsiI CCGC 4 cut(s) 24, 126, 153, 855
SspMI CTAG 3 cut(s) 668, 836, 843
StyD4I CCNGG 5 cut(s) 168, 174, 356, 567, 923
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 2 cut(s) 15, 704
TaqI TCGA 4 cut(s) 273, 409, 808, 974
TasI AATT 4 cut(s) 50, 306, 713, 868
TatI WGTACW 1 cut(s) 740
Tru1I TTAA 2 cut(s) 486, 960
Tru9I TTAA 2 cut(s) 486, 960
TscAI CASTG 1 cut(s) 883
TseI GCWGC 4 cut(s) 26, 143, 287, 353
TspDTI ATGAA 2 cut(s) 605, 757
TspGWI ACGGA 2 cut(s) 147, 151
TspRI CASTG 1 cut(s) 883
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 1 cut(s) 713
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 2 cut(s) 165, 195
XspI CTAG 3 cut(s) 668, 836, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.