pycom12g22690

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006115
Physical Location & Seq
Forward (+)
23538438 .. 23541143
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g22690.1

Sequence Viewer

Length: 735 bp
ATGGCGAACCCACCGTCTTCGTCCGCAGCTCCTCCGTTGACCTCTTCCCAATTCACATACGCGAACGCCTCTTCGTCATCGTCCTACTTCCCCATGCCTTTCCACCTCCAGCAACCCCAGTACCCCGCGCCTTACGCCGTTGCAGCGCCACCCGCTCCGTCCGTCTACCCGGCCCCGGCTCCGGTCGCCTGTGTCTACTCTCTTCCTCAGCAGTATCAGCAGGCTCAGCAGTTGTTTCAAAGGGATGCGCAGACGATTACACCTGAGGCTCTCGAGAGTGTGAAGGCTGCTCTTGCAAACAGCGAAATTGAGCACAAAGCGGAAGCTAAGAAGAAAGCTATCCCTCGTAAAGCTGCTGGGCAGGCTTGGGAGGACCCAACTCTTGCAGAATGGCCAGAAAATGATTATCGACTTTTTTGTGGCGATCTTGGTAATGAGGTGAATGATGATGTACTTTCAAAAGCATTTTCACGATTTCCATCCTTTAACATGGCAAGAGTTGTAAGAGATAAGAGGACTGGAAAAACCAAGGGCTTTGGATTTGTTAGCTTTGCCAACCCATCTGACCTGGCCGGAGCACTAAAGGAAATGAATGGTAAGTATGTGGGCAATCGGCCAATCAAACTACGCAAGAGTAACTGGAGGGAGAGGATAGACTATGACGCACTAGAAAGACAAAAGAACCACACTCACAAGAAACCAAAGCCTTCGAAGAAGAGTATATTGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

245

Amino Acids

27.08

Weight (kDa)

9.57

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 138 - 208 1e-18 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 249
AccBSI CCGCTC 1 cut(s) 155
AccI GTMKAC 2 cut(s) 165, 195
AccII CGCG 2 cut(s) 62, 128
AciI CCGC 4 cut(s) 24, 126, 153, 320
AcoI YGGCCR 3 cut(s) 392, 570, 614
AfaI GTAC 2 cut(s) 122, 453
AfiI CCNNNNNNNGG 2 cut(s) 175, 181
AgsI TTSAA 2 cut(s) 239, 459
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 5 cut(s) 29, 326, 338, 353, 549
AluI AGCT 5 cut(s) 29, 326, 338, 353, 549
Alw21I GWGCWC 2 cut(s) 315, 580
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 4 cut(s) 171, 392, 570, 614
ApeKI GCWGC 4 cut(s) 26, 143, 287, 353
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 3 cut(s) 130, 148, 250
AspS9I GGNCC 2 cut(s) 172, 373
AsuC2I CCSGG 2 cut(s) 170, 176
AsuHPI GGTGA 1 cut(s) 451
AsuII TTCGAA 1 cut(s) 710
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 373
AxyI CCTNAGG 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 104, 137
BalI TGGCCA 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 315, 580
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 38, 155, 274, 340
BccI CCATC 2 cut(s) 487, 568
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 569
BcnI CCSGG 2 cut(s) 170, 176
BfaI CTAG 1 cut(s) 668
BfoI RGCGCY 1 cut(s) 149
BisI GCNGC 4 cut(s) 27, 144, 288, 354
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 4 cut(s) 28, 145, 289, 355
Bme1390I CCNGG 3 cut(s) 170, 176, 569
Bme18I GGWCC 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 172, 373
BmiI GGNNCC 3 cut(s) 174, 180, 375
BmrFI CCNGG 3 cut(s) 170, 176, 569
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 92, 661
Bpu10I CCTNAGC 1 cut(s) 207
Bpu1102I GCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 710
BpuMI CCSGG 2 cut(s) 170, 176
BsaBI GATNNNNATC 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 174, 528
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 181
Bse1I ACTGG 3 cut(s) 118, 523, 644
Bse21I CCTNAGG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 478
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 2 cut(s) 174, 528
BseGI GGATG 2 cut(s) 250, 479
BseJI GATNNNNATC 1 cut(s) 478
BseLI CCNNNNNNNGG 2 cut(s) 175, 181
BseMII CTCAG 3 cut(s) 221, 239, 255
BseNI ACTGG 3 cut(s) 118, 523, 644
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 4 cut(s) 38, 155, 274, 340
BseYI CCCAGC 1 cut(s) 356
Bsh1236I CGCG 2 cut(s) 62, 128
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 4 cut(s) 173, 394, 572, 616
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 315, 580
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 4 cut(s) 170, 176, 182, 573
BslI CCNNNNNNNGG 2 cut(s) 175, 181
BsnI GGCC 4 cut(s) 173, 394, 572, 616
BsoBI CYCGRG 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 710
Bsp1286I GDGCHC 2 cut(s) 315, 580
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 4 cut(s) 24, 126, 153, 320
BspANI GGCC 4 cut(s) 173, 394, 572, 616
BspCNI CTCAG 3 cut(s) 220, 238, 256
BspFNI CGCG 2 cut(s) 62, 128
BspLI GGNNCC 3 cut(s) 174, 180, 375
BspT104I TTCGAA 1 cut(s) 710
BsrBI CCGCTC 1 cut(s) 155
BsrI ACTGG 3 cut(s) 118, 523, 644
BssECI CCNNGG 2 cut(s) 174, 528
BssMI GATC 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 1 cut(s) 15
Bst6I CTCTTC 4 cut(s) 49, 76, 207, 710
BstBI TTCGAA 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 222, 363
BstDEI CTNAG 4 cut(s) 207, 225, 264, 327
BstF5I GGATG 2 cut(s) 250, 479
BstFNI CGCG 2 cut(s) 62, 128
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 130, 148, 250
BstKTI GATC 1 cut(s) 427
BstMBI GATC 1 cut(s) 424
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 3 cut(s) 168, 174, 567
BstUI CGCG 2 cut(s) 62, 128
BstV1I GCAGC 4 cut(s) 38, 155, 274, 340
BstV2I GAAGAC 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 4 cut(s) 173, 394, 572, 616
BtsCI GGATG 2 cut(s) 250, 479
Cac8I GCNNGC 2 cut(s) 222, 363
CfoI GCGC 3 cut(s) 130, 148, 250
Cfr13I GGNCC 2 cut(s) 172, 373
CseI GACGC 1 cut(s) 671
Csp6I GTAC 2 cut(s) 121, 452
CviAII CATG 2 cut(s) 94, 490
CviQI GTAC 2 cut(s) 121, 452
DdeI CTNAG 4 cut(s) 207, 225, 264, 327
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 3 cut(s) 392, 570, 614
Eam1104I CTCTTC 4 cut(s) 49, 76, 207, 710
EarI CTCTTC 4 cut(s) 49, 76, 207, 710
Eco130I CCWWGG 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 373
Eco81I CCTNAGG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 2 cut(s) 97, 493
FaiI YATR 6 cut(s) 58, 95, 491, 603, 660, 722
FatI CATG 2 cut(s) 93, 489
FauI CCCGC 2 cut(s) 133, 160
FblI GTMKAC 2 cut(s) 165, 195
Fnu4HI GCNGC 4 cut(s) 27, 144, 288, 354
FokI GGATG 2 cut(s) 257, 466
Fsp4HI GCNGC 4 cut(s) 27, 144, 288, 354
FspBI CTAG 1 cut(s) 668
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 3 cut(s) 129, 147, 249
GluI GCNGC 4 cut(s) 27, 144, 288, 354
GsaI CCCAGC 1 cut(s) 360
GsuI CTGGAG 2 cut(s) 92, 661
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 4 cut(s) 173, 394, 572, 616
HapII CCGG 4 cut(s) 170, 176, 182, 573
HgaI GACGC 1 cut(s) 671
HhaI GCGC 3 cut(s) 130, 148, 250
Hin1II CATG 2 cut(s) 97, 493
Hin6I GCGC 3 cut(s) 128, 146, 248
HinP1I GCGC 3 cut(s) 128, 146, 248
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HpaII CCGG 4 cut(s) 170, 176, 182, 573
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 3 cut(s) 39, 166, 196
Hpy188I TCNGA 1 cut(s) 565
Hpy188III TCNNGA 3 cut(s) 272, 274, 471
Hpy8I GTNNAC 3 cut(s) 39, 166, 196
HpyAV CCTTC 2 cut(s) 277, 717
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 4 cut(s) 143, 296, 386, 727
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
HpyF3I CTNAG 4 cut(s) 207, 225, 264, 327
Hsp92II CATG 2 cut(s) 97, 493
HspAI GCGC 3 cut(s) 128, 146, 248
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 4 cut(s) 34, 160, 184, 575
Lsp1109I GCAGC 4 cut(s) 38, 155, 274, 340
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 668
MaeIII GTNAC 1 cut(s) 635
MalI GATC 1 cut(s) 426
MbiI CCGCTC 1 cut(s) 155
MboI GATC 1 cut(s) 424
MboII GAAGA 7 cut(s) 9, 36, 63, 194, 343, 724, 727
MhlI GDGCHC 2 cut(s) 315, 580
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 2 cut(s) 50, 306
MluNI TGGCCA 1 cut(s) 394
Mox20I TGGCCA 1 cut(s) 394
MscI TGGCCA 1 cut(s) 394
MseI TTAA 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 394
MspI CCGG 4 cut(s) 170, 176, 182, 573
MspR9I CCNGG 3 cut(s) 170, 176, 569
MvaI CCWGG 1 cut(s) 569
MvnI CGCG 2 cut(s) 62, 128
MwoI GCNNNNNNNGC 8 cut(s) 134, 143, 152, 185, 217, 226, 293, 362
NciI CCSGG 2 cut(s) 170, 176
NdeII GATC 1 cut(s) 424
NlaIII CATG 2 cut(s) 97, 493
NlaIV GGNNCC 3 cut(s) 174, 180, 375
NsbI TGCGCA 1 cut(s) 249
NspV TTCGAA 1 cut(s) 710
PaeR7I CTCGAG 1 cut(s) 272
PkrI GCNGC 4 cut(s) 28, 145, 289, 355
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 174, 180, 375
PspPI GGNCC 2 cut(s) 172, 373
PspPPI RGGWCCY 1 cut(s) 373
RsaI GTAC 2 cut(s) 122, 453
RsaNI GTAC 2 cut(s) 121, 452
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 4 cut(s) 27, 144, 288, 354
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 2 cut(s) 172, 373
ScrFI CCNGG 3 cut(s) 170, 176, 569
SduI GDGCHC 2 cut(s) 315, 580
SfaNI GCATC 1 cut(s) 235
Sfr274I CTCGAG 1 cut(s) 272
SfuI TTCGAA 1 cut(s) 710
SinI GGWCC 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 50, 306
SsiI CCGC 4 cut(s) 24, 126, 153, 320
SspMI CTAG 1 cut(s) 668
StyD4I CCNGG 3 cut(s) 168, 174, 567
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 15
TaqI TCGA 3 cut(s) 273, 409, 710
TasI AATT 2 cut(s) 50, 306
TatI WGTACW 1 cut(s) 451
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseI GCWGC 4 cut(s) 26, 143, 287, 353
TspDTI ATGAA 1 cut(s) 605
TspGWI ACGGA 3 cut(s) 24, 147, 151
VpaK11BI GGWCC 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 2 cut(s) 165, 195
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.