Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009217

Physical Location & Seq

Genomic Coordinates Reverse (-)

2396716 .. 2405461

Gene Structure 8746 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1012000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGGTGCCTTCGGGGCCGCCCACTCCAATTGGTGGTGCCCAGTCTGTTTCGCCTTCACTTTTGCGTTCAAATTCTGGAATGTTGGGAGGTCAAGGTTCCCAGTCGGCTTTTCCCTCGCTTGCCTCACCACGAACTCAATATGGTAATATGCTTGGCAATGTAGCCAATGTGTCTTCTCTCCTTAATCAGTCGTATGGAAATGGAATTTCGAATCCAGGGCTTTCTGGTCCTGGTAGCAACCAACGTGGAGGTATGGATACTGGTGCTGAATCTGATCCACTTTCTGGTGTTGGCAATGGAATGGGTTTCAATGCACCTTCTTTGTCATACGGTGCATCAAACATGGCTAATCCCGGTACGTCTGGTCATGGTCATGGTCAAGGGCAAGGGCAAGGGCAACAATTTTCAAACCCTTCTTGTAACCAACTGTTGACGGAACAACAGCAGCAGCAACTTGAAACACAGCATTTCCAGCATGGTCAGCAGCCAATGCAACAGTTCTCTGCCCCTCACAATACGCAGCAGCAGCAGCATCAATTTCAGGCTATTCGAGGAGGCTTAGCTCGTGTTGGGCCGGTAAAGTTAGAGCCCCAATTGACAAATGATCAACATGGACAGCAGCAGCAACAGCAGCAGGTGCAGTCTTTGAGTCTTGGTCCAGTGAAAATGGAACCCCAACAACTTCAAACTATGAGAAGCTTGCCACCTGTAAAATTGGAACCGCAGCAATCTGATCAGTCATTGTTTTTGCATCAACAGCAGCAACAACAGCTCCTCCACATGTCAAGGCAGCCTTCTCAGAATGCTGCTGCCCAGATGAACATCTTGCATCAGCAGAGATTATTGCAGTTACAACAGCAACAACACCAGCAACAGCAACAACACCAGCAGCAGCAATTCTTGAAAGCAATGCCTCAACAACGCCCCCAGTTACAGCAGCAGTTCCCGCAGCAGAATCTGCCTATGAGATCTCCTGCGAAACCAGTATATGAGCCTGGAATGTGTGCTCGGCGTTTGACACATTATATGTATCAGCAACAGCACAGGCCTGAAGACAACAACATTGAATTCTGGAGAAAATTTGTTGCTGAATATTTTGTACCCCATGCGAAGAAGAAGTGGTGCGTTTCTATGTATGGAACTGGCCGACAAACAACTGGCGTTTTTCCTCAGGATGTATGGCACTGTGAAATATGCAATCGCAAGCCTGGGCGTGGCTTTGAAGCGACCGTTGAGGTTCTTCCCCGGCTTTTTAAAATCAAGTATGAAAGTGGTACATTGGAGGAGCTTCTTTATGTTGATATGCCGCGCGAGTATCATAATTCATCTGGCCAGATTGTGTTGGACTATGCTAAAGCAATACAAGAAAGTGTTTTTGAGCAACTTCGTGTTGTTCGGGATGGCCAACTTCGGATAGTTTTCTCTCCAGATCTGAAGATATGCTCTTGGGAATTTTGTGCAAGGCGCCATGAAGAGCTTATCCCTCGAAGATTACTGATACCTCAGGTTAGTCAGCTTGGTGCTGCAGCTCAAAAATACCAGGCTGCTACTCAAAATGCATCATCCAATATATCTCTTCCAGAGATACAAAGTAACTTGGTGTTTTTGCCTGACAAGGTTTGGGCTTTCAAATGGTTTTTCTTTTATAGAAGTTTGGCTATAATCGATGTTCATGGCTTGTTTGTATCATCAGCGCGGCAGTTGGCAAAAACCTTGGAAGTGCCTTTAGTAAATGATTTAGGTTATACAAAGCGATACGTAAGGTGTCTTCAGATATCAGAAGTAGTTAATAGTATGAAAGACTTGATTGATTACAGCCGAGAGACAGGGACTGGACCTATGGAGAGCTTGGCCAAGTTTCCTCGAAGGACGAGTGCTTCAACTGGGTTTCACTGTCAAGCACAACAGTCCGAGGAGCAAATGCAGCAGCAGCAACAACAAACGATCGCCCAGAATTCAAATGGAGATCCAAGCTCTGTTCAGGCCGCTGCTACGCAAATTGCTGCCAGTAATGGTGTTGCTAGTGTAAATAATGCTCTGAACACAGCATCCACATCTACTTCTGCAAGCACCATTGTTGGGCTCCTCCATCAGAATTCTATGAACTCCAGACAGCAAAGTTCTATGAATAACGCAAACAGTCCATATGGAGGAAGTTCTGTTCAGATTCCATCCCCTGGTTCTTCTAGTACAATCCCACAAACACAACCAAACCCTTCTCCATTCCAGTCACCAACACCCTCATCAAATAATCCCTCTCAAACATCTCAAGGTGTCATGACAGCTGCCAATCACATGGGTGCAGCAAATTCACCAGCAAATATATCCATGCAACAGCCAACCCTTTCGGGTGAGGCTGATCCAAGTGATTCCCAGAGCTCTGTCCAGAAAATTTTACATGAAATGATGATGTCCAACCAGCTTAGCGGCCCAGGTGGTATGGTTGGTGCTGGTTCTTTGGGCAACGATCTGAAAAACATGAATGGGATTTTGTCAACAAGCAACAACACAGGATTGAATGGCATGACTAACAATAACTCAGGAATTGGGGGTGCTGGATTTGGGAGTATGGGTGGCGGACTAGGTCAGCAGCCTTCCATGGCGAATGGAATAAGAGCAGCAATGGGAAATAATTCTGTTATGAATGGAAGGGTCGGAATGGCTCGAGAACAAAGTATGCACCATCAACAACAGGATATGGGGAACCAGTTACTTAGTGGGCTTGGAGCGGTGAACGGCTTTAATAATCTTCAATATGATTGGAAACATTCTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009909 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0010243 GO:0010272 GO:0014070 GO:0022414 GO:0032501 GO:0032502 GO:0033554 GO:0035670 GO:0036293 GO:0042221 GO:0042493 GO:0043207 GO:0046677 GO:0046898 GO:0046982 GO:0046983 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048580 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060090 GO:0060992 GO:0061458 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0090567 GO:0097159 GO:0097305 GO:0099402 GO:1901363 GO:1901654 GO:1901698 GO:1901700 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LIM_bind

Protein Analysis

926

Amino Acids

100.91

Weight (kDa)

8.89

Isoelectric Point (pI)

58.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LIM_bind	PF01803	338 - 617	4.7e-65	LIM-domain binding protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43850 AT1G43850
fragaria_vesca	FvH4_2g14890
malus_domestica	MD05G1012000.v1.1 MD10G1012000.v1.1
prunus_persica	Prupe.8G017000_v2.0.a1 Prupe.8G017000_v2.0.a1 Prupe.8G017000_v2.0.a1
pyrus_communis	pycom05g00700 pycom10g00760
rosa_chinensis	RchiOBHm_Chr6g0277461
rosa_laevigata	RLG00000013299
rosa_multiflora	Rmu_co8465657.1_g000001 Rmu_sc0003910.1_g000012
rosa_roxburghii	Rroxscaffold_7G00191290
rosa_rugosa	Rorug06G0108400 Rorug06G0108500
rosa_samantha	Rh6AG219300 Rh6BG223200 Rh6CG225700 Rh6DG216000
rosa_wichuraiana	Rw6G019200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	625
Acc36I	ACCTGC	1 cut(s)	625
AccB1I	GGYRCC	3 cut(s)	4, 35, 1464
AccB7I	CCANNNNNTGG	3 cut(s)	32, 284, 2177
AccBSI	CCGCTC	1 cut(s)	2729
AccII	CGCG	3 cut(s)	1309, 1311, 1696
AciI	CCGC	9 cut(s)	17, 722, 947, 1307, 1696, 1986, 2427, 2577, 2729
AclWI	GGATC	3 cut(s)	269, 1961, 2354
AcoI	YGGCCR	4 cut(s)	1144, 1330, 1402, 1851
AcsI	RAATTY	9 cut(s)	70, 204, 1067, 1079, 1451, 1954, 2095, 2308, 2391
AcuI	CTGAAG	3 cut(s)	1071, 1454, 1754
AcyI	GRCGYC	1 cut(s)	1465
AfaI	GTAC	4 cut(s)	358, 1101, 1276, 2191
AfiI	CCNNNNNNNGG	6 cut(s)	32, 284, 1214, 2079, 2150, 2177
AflIII	ACRYGT	1 cut(s)	780
AjnI	CCWGG	7 cut(s)	214, 229, 994, 1207, 1539, 2176, 2431
AleI	CACNNNNGTG	1 cut(s)	2298
AloI	GAACNNNNNNTCC	4 cut(s)	2033, 2065, 2145, 2177
Alw21I	GWGCWC	2 cut(s)	1009, 2381
Alw26I	GTCTC	1 cut(s)	1817
AlwI	GGATC	3 cut(s)	269, 1961, 2354
AlwNI	CAGNNNCTG	2 cut(s)	958, 1832
Ama87I	CYCGRG	1 cut(s)	2664
AoxI	GGCC	9 cut(s)	14, 572, 1046, 1144, 1330, 1402, 1851, 1983, 2428
ApoI	RAATTY	9 cut(s)	70, 204, 1067, 1079, 1451, 1954, 2095, 2308, 2391
Asp700I	GAANNNNTTC	1 cut(s)	2632
AspLEI	GCGC	3 cut(s)	1311, 1467, 1696
AspS9I	GGNCC	6 cut(s)	14, 227, 572, 656, 1835, 2429
AsuC2I	CCSGG	2 cut(s)	354, 1246
AsuHPI	GGTGA	5 cut(s)	117, 2223, 2304, 2363, 2743
AsuII	TTCGAA	1 cut(s)	209
AvaI	CYCGRG	1 cut(s)	2664
AvaII	GGWCC	3 cut(s)	227, 656, 1835
AxyI	CCTNAGG	2 cut(s)	1170, 1503
BaeGI	GKGCMC	1 cut(s)	40
BalI	TGGCCA	3 cut(s)	1332, 1404, 1853
BanI	GGYRCC	3 cut(s)	4, 35, 1464
BanII	GRGCYC	3 cut(s)	591, 2085, 2381
BarI	GAAGNNNNNNTAC	2 cut(s)	1752, 1784
BauI	CACGAG	1 cut(s)	564
BbsI	GAAGAC	3 cut(s)	165, 1059, 1760
Bbv12I	GWGCWC	2 cut(s)	1009, 2381
BccI	CCATC	4 cut(s)	1394, 2097, 2179, 2691
BceAI	ACGGC	1 cut(s)	2752
BciT130I	CCWGG	7 cut(s)	216, 231, 996, 1209, 1541, 2178, 2433
BciVI	GTATCC	1 cut(s)	250
BclI	TGATCA	2 cut(s)	604, 733
BcnI	CCSGG	2 cut(s)	354, 1246
BcoDI	GTCTC	1 cut(s)	1817
BfaI	CTAG	3 cut(s)	2022, 2187, 2582
BfmI	CTRYAG	1 cut(s)	1524
BfoI	RGCGCY	1 cut(s)	1468
BfuAI	ACCTGC	1 cut(s)	625
BfuI	GTATCC	1 cut(s)	250
BglI	GCCNNNNNGGC	1 cut(s)	13
BglII	AGATCT	2 cut(s)	968, 1429
BlpI	GCTNAGC	2 cut(s)	559, 2423
Bme1390I	CCNGG	9 cut(s)	216, 231, 354, 996, 1209, 1246, 1541, 2178, 2433
Bme18I	GGWCC	3 cut(s)	227, 656, 1835
BmeT110I	CYCGRG	1 cut(s)	2664
BmgT120I	GGNCC	6 cut(s)	14, 227, 572, 656, 1835, 2429
BmiI	GGNNCC	9 cut(s)	6, 15, 37, 97, 672, 720, 1466, 2084, 2705
BmrFI	CCNGG	9 cut(s)	216, 231, 354, 996, 1209, 1246, 1541, 2178, 2433
BmrI	ACTGGG	4 cut(s)	34, 94, 922, 1893
BmsI	GCATC	6 cut(s)	344, 541, 760, 838, 1568, 2057
BmuI	ACTGGG	4 cut(s)	34, 94, 922, 1893
BpiI	GAAGAC	3 cut(s)	165, 1059, 1760
BpmI	CTGGAG	3 cut(s)	1093, 1410, 2092
Bpu1102I	GCTNAGC	2 cut(s)	559, 2423
Bpu14I	TTCGAA	1 cut(s)	209
BpuEI	CTTGAG	1 cut(s)	2253
BpuMI	CCSGG	2 cut(s)	354, 1246
Bsa29I	ATCGAT	1 cut(s)	1665
BsaAI	YACGTR	1 cut(s)	1759
BsaBI	GATNNNNATC	1 cut(s)	821
BsaHI	GRCGYC	1 cut(s)	1465
BsaJI	CCNNGG	8 cut(s)	215, 1208, 1244, 1713, 1911, 2176, 2431, 2598
BsaXI	ACNNNNNCTCC	6 cut(s)	756, 759, 786, 789, 2557, 2587
Bsc4I	CCNNNNNNNGG	6 cut(s)	32, 284, 1214, 2079, 2150, 2177
Bse118I	RCCGGY	1 cut(s)	574
Bse21I	CCTNAGG	2 cut(s)	1170, 1503
Bse3DI	GCAATG	4 cut(s)	163, 301, 915, 2628
Bse8I	GATNNNNATC	1 cut(s)	821
BseBI	CCWGG	7 cut(s)	216, 231, 996, 1209, 1541, 2178, 2433
BseCI	ATCGAT	1 cut(s)	1665
BseDI	CCNNGG	8 cut(s)	215, 1208, 1244, 1713, 1911, 2176, 2431, 2598
BseGI	GGATG	5 cut(s)	1180, 1405, 1562, 2048, 2171
BseJI	GATNNNNATC	1 cut(s)	821
BseLI	CCNNNNNNNGG	6 cut(s)	32, 284, 1214, 2079, 2150, 2177
BseMI	GCAATG	4 cut(s)	163, 301, 915, 2628
BseMII	CTCAG	4 cut(s)	812, 1184, 1517, 2553
BseRI	GAGGAG	5 cut(s)	567, 764, 1298, 1928, 2075
BseSI	GKGCMC	1 cut(s)	40
BsgI	GTGCAG	2 cut(s)	659, 2322
Bsh1236I	CGCG	3 cut(s)	1309, 1311, 1696
Bsh1285I	CGRYCG	2 cut(s)	1230, 1947
BshFI	GGCC	9 cut(s)	16, 574, 1048, 1146, 1332, 1404, 1853, 1985, 2430
BshNI	GGYRCC	3 cut(s)	4, 35, 1464
BshVI	ATCGAT	1 cut(s)	1665
BsiEI	CGRYCG	2 cut(s)	1230, 1947
BsiHKAI	GWGCWC	2 cut(s)	1009, 2381
BsiHKCI	CYCGRG	1 cut(s)	2664
BsiSI	CCGG	3 cut(s)	354, 575, 1246
BslFI	GGGAC	1 cut(s)	1843
BslI	CCNNNNNNNGG	6 cut(s)	32, 284, 1214, 2079, 2150, 2177
BsmAI	GTCTC	1 cut(s)	1817
BsmFI	GGGAC	1 cut(s)	1843
BsmI	GAATGC	1 cut(s)	808
BsnI	GGCC	9 cut(s)	16, 574, 1048, 1146, 1332, 1404, 1853, 1985, 2430
BsoBI	CYCGRG	1 cut(s)	2664
Bsp119I	TTCGAA	1 cut(s)	209
Bsp1286I	GDGCHC	5 cut(s)	40, 591, 1009, 2085, 2381
Bsp143I	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
Bsp1720I	GCTNAGC	2 cut(s)	559, 2423
Bsp19I	CCATGG	1 cut(s)	2598
BspACI	CCGC	9 cut(s)	17, 722, 947, 1307, 1696, 1986, 2427, 2577, 2729
BspANI	GGCC	9 cut(s)	16, 574, 1048, 1146, 1332, 1404, 1853, 1985, 2430
BspCNI	CTCAG	4 cut(s)	811, 1183, 1516, 2552
BspDI	ATCGAT	1 cut(s)	1665
BspFNI	CGCG	3 cut(s)	1309, 1311, 1696
BspHI	TCATGA	1 cut(s)	2277
BspLI	GGNNCC	9 cut(s)	6, 15, 37, 97, 672, 720, 1466, 2084, 2705
BspMAI	CTGCAG	1 cut(s)	1528
BspMI	ACCTGC	1 cut(s)	625
BspPI	GGATC	3 cut(s)	269, 1961, 2354
BspQI	GCTCTTC	1 cut(s)	1467
BspT104I	TTCGAA	1 cut(s)	209
BspT107I	GGYRCC	3 cut(s)	4, 35, 1464
BsrBI	CCGCTC	1 cut(s)	2729
BsrDI	GCAATG	4 cut(s)	163, 301, 915, 2628
BsrFI	RCCGGY	1 cut(s)	574
BssAI	RCCGGY	1 cut(s)	574
BssECI	CCNNGG	8 cut(s)	215, 1208, 1244, 1713, 1911, 2176, 2431, 2598
BssMI	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
BssNI	GRCGYC	1 cut(s)	1465
BssSI	CACGAG	1 cut(s)	564
BssT1I	CCWWGG	2 cut(s)	1713, 2598
Bst2BI	CACGAG	1 cut(s)	564
Bst2UI	CCWGG	7 cut(s)	216, 231, 996, 1209, 1541, 2178, 2433
Bst4CI	ACNGT	8 cut(s)	332, 429, 498, 1187, 1231, 1895, 1908, 2141
Bst6I	CTCTTC	2 cut(s)	1467, 1581
BstACI	GRCGYC	1 cut(s)	1465
BstAPI	GCANNNNNTGC	3 cut(s)	490, 637, 958
BstBAI	YACGTR	1 cut(s)	1759
BstBI	TTCGAA	1 cut(s)	209
BstC8I	GCNNGC	4 cut(s)	120, 701, 1205, 2068
BstDEI	CTNAG	7 cut(s)	559, 798, 1170, 1503, 2423, 2539, 2714
BstDSI	CCRYGG	1 cut(s)	2598
BstF5I	GGATG	5 cut(s)	1180, 1405, 1562, 2048, 2171
BstFNI	CGCG	3 cut(s)	1309, 1311, 1696
BstH2I	RGCGCY	1 cut(s)	1468
BstHHI	GCGC	3 cut(s)	1311, 1467, 1696
BstKTI	GATC	9 cut(s)	277, 607, 736, 971, 1432, 1947, 1969, 2362, 2470
BstMAI	GTCTC	1 cut(s)	1817
BstMBI	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
BstMCI	CGRYCG	2 cut(s)	1230, 1947
BstNI	CCWGG	7 cut(s)	216, 231, 996, 1209, 1541, 2178, 2433
BstNSI	RCATGY	1 cut(s)	784
BstSCI	CCNGG	9 cut(s)	214, 229, 352, 994, 1207, 1244, 1539, 2176, 2431
BstSFI	CTRYAG	1 cut(s)	1524
BstSLI	GKGCMC	1 cut(s)	40
BstSNI	TACGTA	1 cut(s)	1759
BstUI	CGCG	3 cut(s)	1309, 1311, 1696
BstV2I	GAAGAC	3 cut(s)	165, 1059, 1760
BstX2I	RGATCY	3 cut(s)	968, 1429, 1966
BstXI	CCANNNNNNTGG	1 cut(s)	2296
BstYI	RGATCY	3 cut(s)	968, 1429, 1966
Bsu15I	ATCGAT	1 cut(s)	1665
Bsu36I	CCTNAGG	2 cut(s)	1170, 1503
BsuI	GTATCC	1 cut(s)	250
BsuRI	GGCC	9 cut(s)	16, 574, 1048, 1146, 1332, 1404, 1853, 1985, 2430
BsuTUI	ATCGAT	1 cut(s)	1665
BtgI	CCRYGG	1 cut(s)	2598
BtsCI	GGATG	5 cut(s)	1180, 1405, 1562, 2048, 2171
BtsIMutI	CAGTG	3 cut(s)	666, 1183, 1891
BveI	ACCTGC	1 cut(s)	625
Cac8I	GCNNGC	4 cut(s)	120, 701, 1205, 2068
CaiI	CAGNNNCTG	2 cut(s)	958, 1832
CciI	TCATGA	1 cut(s)	2277
CfoI	GCGC	3 cut(s)	1311, 1467, 1696
Cfr10I	RCCGGY	1 cut(s)	574
Cfr13I	GGNCC	6 cut(s)	14, 227, 572, 656, 1835, 2429
ClaI	ATCGAT	1 cut(s)	1665
Csp6I	GTAC	4 cut(s)	357, 1100, 1275, 2190
CspCI	CAANNNNNGTGG	4 cut(s)	10, 45, 226, 261
CviQI	GTAC	4 cut(s)	357, 1100, 1275, 2190
DdeI	CTNAG	7 cut(s)	559, 798, 1170, 1503, 2423, 2539, 2714
DinI	GGCGCC	1 cut(s)	1466
DpnI	GATC	9 cut(s)	276, 606, 735, 970, 1431, 1946, 1968, 2361, 2469
DpnII	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
DraI	TTTAAA	1 cut(s)	1255
EaeI	YGGCCR	4 cut(s)	1144, 1330, 1402, 1851
Eam1104I	CTCTTC	2 cut(s)	1467, 1581
EarI	CTCTTC	2 cut(s)	1467, 1581
EciI	GGCGGA	1 cut(s)	2592
Ecl136II	GAGCTC	1 cut(s)	2379
Eco105I	TACGTA	1 cut(s)	1759
Eco130I	CCWWGG	2 cut(s)	1713, 2598
Eco147I	AGGCCT	1 cut(s)	1048
Eco24I	GRGCYC	3 cut(s)	591, 2085, 2381
Eco32I	GATATC	1 cut(s)	1776
Eco47I	GGWCC	3 cut(s)	227, 656, 1835
Eco53kI	GAGCTC	1 cut(s)	2379
Eco57I	CTGAAG	3 cut(s)	1071, 1454, 1754
Eco81I	CCTNAGG	2 cut(s)	1170, 1503
Eco88I	CYCGRG	1 cut(s)	2664
EcoICRI	GAGCTC	1 cut(s)	2379
EcoRI	GAATTC	3 cut(s)	1067, 1954, 2095
EcoRII	CCWGG	7 cut(s)	214, 229, 994, 1207, 1539, 2176, 2431
EcoRV	GATATC	1 cut(s)	1776
EcoT14I	CCWWGG	2 cut(s)	1713, 2598
EcoT22I	ATGCAT	1 cut(s)	1561
EcoT38I	GRGCYC	3 cut(s)	591, 2085, 2381
EgeI	GGCGCC	1 cut(s)	1466
EheI	GGCGCC	1 cut(s)	1466
ErhI	CCWWGG	2 cut(s)	1713, 2598
FalI	AAGNNNNNCTT	2 cut(s)	778, 810
FaqI	GGGAC	1 cut(s)	1843
FauI	CCCGC	1 cut(s)	954
FauNDI	CATATG	1 cut(s)	2146
FbaI	TGATCA	2 cut(s)	604, 733
FokI	GGATG	5 cut(s)	1187, 1412, 1549, 2035, 2158
FriOI	GRGCYC	3 cut(s)	591, 2085, 2381
FspBI	CTAG	3 cut(s)	2022, 2187, 2582
GlaI	GCGC	3 cut(s)	1310, 1466, 1695
GsuI	CTGGAG	3 cut(s)	1093, 1410, 2092
HaeII	RGCGCY	1 cut(s)	1468
HaeIII	GGCC	9 cut(s)	16, 574, 1048, 1146, 1332, 1404, 1853, 1985, 2430
HapII	CCGG	3 cut(s)	354, 575, 1246
HhaI	GCGC	3 cut(s)	1311, 1467, 1696
Hin1I	GRCGYC	1 cut(s)	1465
Hin6I	GCGC	3 cut(s)	1309, 1465, 1694
HinP1I	GCGC	3 cut(s)	1309, 1465, 1694
HincII	GTYRAC	2 cut(s)	432, 2496
HindII	GTYRAC	2 cut(s)	432, 2496
HindIII	AAGCTT	1 cut(s)	697
HinfI	GANTC	6 cut(s)	211, 269, 649, 955, 2167, 2369
HpaII	CCGG	3 cut(s)	354, 575, 1246
HphI	GGTGA	5 cut(s)	117, 2223, 2304, 2363, 2743
Hpy166II	GTNNAC	3 cut(s)	432, 2496, 2734
Hpy8I	GTNNAC	3 cut(s)	432, 2496, 2734
HpyAV	CCTTC	9 cut(s)	18, 63, 327, 423, 804, 1860, 2226, 2604, 2643
HpyCH4III	ACNGT	8 cut(s)	332, 429, 498, 1187, 1231, 1895, 1908, 2141
HpyCH4IV	ACGT	3 cut(s)	244, 359, 1758
HpyF3I	CTNAG	7 cut(s)	559, 798, 1170, 1503, 2423, 2539, 2714
HpySE526I	ACGT	3 cut(s)	244, 359, 1758
Hsp92I	GRCGYC	1 cut(s)	1465
HspAI	GCGC	3 cut(s)	1309, 1465, 1694
KasI	GGCGCC	1 cut(s)	1464
Ksp22I	TGATCA	2 cut(s)	604, 733
Kzo9I	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
LguI	GCTCTTC	1 cut(s)	1467
LmnI	GCTCC	5 cut(s)	777, 1285, 1915, 2088, 2726
LweI	GCATC	6 cut(s)	344, 541, 760, 838, 1568, 2057
MaeI	CTAG	3 cut(s)	2022, 2187, 2582
MaeII	ACGT	3 cut(s)	244, 359, 1758
MaeIII	GTNAC	6 cut(s)	419, 849, 930, 1592, 2229, 2709
MalI	GATC	9 cut(s)	276, 606, 735, 970, 1431, 1946, 1968, 2361, 2469
MbiI	CCGCTC	1 cut(s)	2729
MboI	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
MfeI	CAATTG	2 cut(s)	27, 593
MflI	RGATCY	3 cut(s)	968, 1429, 1966
MhlI	GDGCHC	5 cut(s)	40, 591, 1009, 2085, 2381
MlsI	TGGCCA	3 cut(s)	1332, 1404, 1853
MluNI	TGGCCA	3 cut(s)	1332, 1404, 1853
Mly113I	GGCGCC	1 cut(s)	1465
MlyI	GAGTC	1 cut(s)	658
MmeI	TCCRAC	3 cut(s)	1323, 2439, 2635
Mox20I	TGGCCA	3 cut(s)	1332, 1404, 1853
Mph1103I	ATGCAT	1 cut(s)	1561
MroXI	GAANNNNTTC	1 cut(s)	2632
MscI	TGGCCA	3 cut(s)	1332, 1404, 1853
MseI	TTAA	4 cut(s)	183, 1254, 1788, 2742
MslI	CAYNNNNRTG	2 cut(s)	372, 2298
Msp20I	TGGCCA	3 cut(s)	1332, 1404, 1853
MspA1I	CMGCKG	2 cut(s)	1988, 2285
MspI	CCGG	3 cut(s)	354, 575, 1246
MspR9I	CCNGG	9 cut(s)	216, 231, 354, 996, 1209, 1246, 1541, 2178, 2433
MunI	CAATTG	2 cut(s)	27, 593
Mva1269I	GAATGC	1 cut(s)	808
MvaI	CCWGG	7 cut(s)	216, 231, 996, 1209, 1541, 2178, 2433
MvnI	CGCG	3 cut(s)	1309, 1311, 1696
NarI	GGCGCC	1 cut(s)	1465
NciI	CCSGG	2 cut(s)	354, 1246
NcoI	CCATGG	1 cut(s)	2598
NdeI	CATATG	1 cut(s)	2146
NdeII	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
NlaIV	GGNNCC	9 cut(s)	6, 15, 37, 97, 672, 720, 1466, 2084, 2705
NmeAIII	GCCGAG	2 cut(s)	988, 1844
NmuCI	GTSAC	1 cut(s)	2229
NsiI	ATGCAT	1 cut(s)	1561
NspI	RCATGY	1 cut(s)	784
NspV	TTCGAA	1 cut(s)	209
OliI	CACNNNNGTG	1 cut(s)	2298
PaeR7I	CTCGAG	1 cut(s)	2664
PagI	TCATGA	1 cut(s)	2277
PaqCI	CACCTGC	1 cut(s)	625
PceI	AGGCCT	1 cut(s)	1048
PciI	ACATGT	1 cut(s)	780
PciSI	GCTCTTC	1 cut(s)	1467
PctI	GAATGC	1 cut(s)	808
PdmI	GAANNNNTTC	1 cut(s)	2632
PfeI	GAWTC	5 cut(s)	211, 269, 955, 2167, 2369
PflFI	GACNNNGTC	1 cut(s)	2583
PflMI	CCANNNNNTGG	3 cut(s)	32, 284, 2177
Ple19I	CGATCG	1 cut(s)	1947
PleI	GAGTC	1 cut(s)	657
PluTI	GGCGCC	1 cut(s)	1468
PpsI	GAGTC	1 cut(s)	657
Ppu21I	YACGTR	1 cut(s)	1759
PscI	ACATGT	1 cut(s)	780
Psp124BI	GAGCTC	1 cut(s)	2381
Psp6I	CCWGG	7 cut(s)	214, 229, 994, 1207, 1539, 2176, 2431
PspGI	CCWGG	7 cut(s)	214, 229, 994, 1207, 1539, 2176, 2431
PspN4I	GGNNCC	9 cut(s)	6, 15, 37, 97, 672, 720, 1466, 2084, 2705
PspPI	GGNCC	6 cut(s)	14, 227, 572, 656, 1835, 2429
PstI	CTGCAG	1 cut(s)	1528
PstNI	CAGNNNCTG	2 cut(s)	958, 1832
PsuI	RGATCY	3 cut(s)	968, 1429, 1966
PsyI	GACNNNGTC	1 cut(s)	2583
PvuI	CGATCG	1 cut(s)	1947
PvuII	CAGCTG	1 cut(s)	2285
RsaI	GTAC	4 cut(s)	358, 1101, 1276, 2191
RsaNI	GTAC	4 cut(s)	357, 1100, 1275, 2190
RseI	CAYNNNNRTG	2 cut(s)	372, 2298
SacI	GAGCTC	1 cut(s)	2381
SapI	GCTCTTC	1 cut(s)	1467
SaqAI	TTAA	4 cut(s)	183, 1254, 1788, 2742
Sau3AI	GATC	9 cut(s)	274, 604, 733, 968, 1429, 1944, 1966, 2359, 2467
Sau96I	GGNCC	6 cut(s)	14, 227, 572, 656, 1835, 2429
SchI	GAGTC	1 cut(s)	658
ScrFI	CCNGG	9 cut(s)	216, 231, 354, 996, 1209, 1246, 1541, 2178, 2433
SduI	GDGCHC	5 cut(s)	40, 591, 1009, 2085, 2381
SfaNI	GCATC	6 cut(s)	344, 541, 760, 838, 1568, 2057
SfcI	CTRYAG	1 cut(s)	1524
SfoI	GGCGCC	1 cut(s)	1466
Sfr274I	CTCGAG	1 cut(s)	2664
SfuI	TTCGAA	1 cut(s)	209
SinI	GGWCC	3 cut(s)	227, 656, 1835
SlaI	CTCGAG	1 cut(s)	2664
SmiMI	CAYNNNNRTG	2 cut(s)	372, 2298
SmlI	CTYRAG	2 cut(s)	2268, 2664
SmoI	CTYRAG	2 cut(s)	2268, 2664
SnaBI	TACGTA	1 cut(s)	1759
SseBI	AGGCCT	1 cut(s)	1048
SsiI	CCGC	9 cut(s)	17, 722, 947, 1307, 1696, 1986, 2427, 2577, 2729
SspDI	GGCGCC	1 cut(s)	1464
SspI	AATATT	1 cut(s)	1094
SspMI	CTAG	3 cut(s)	2022, 2187, 2582
SstI	GAGCTC	1 cut(s)	2381
StuI	AGGCCT	1 cut(s)	1048
StyD4I	CCNGG	9 cut(s)	214, 229, 352, 994, 1207, 1244, 1539, 2176, 2431
StyI	CCWWGG	2 cut(s)	1713, 2598
TaaI	ACNGT	8 cut(s)	332, 429, 498, 1187, 1231, 1895, 1908, 2141
TaiI	ACGT	3 cut(s)	247, 362, 1761
TaqI	TCGA	6 cut(s)	209, 550, 1486, 1665, 1864, 2665
TatI	WGTACW	1 cut(s)	2189
TauI	GCSGC	5 cut(s)	19, 1309, 1699, 1988, 2430
TfiI	GAWTC	5 cut(s)	211, 269, 955, 2167, 2369
Tru1I	TTAA	4 cut(s)	183, 1254, 1788, 2742
Tru9I	TTAA	4 cut(s)	183, 1254, 1788, 2742
TscAI	CASTG	3 cut(s)	666, 1190, 1898
TseFI	GTSAC	1 cut(s)	2229
Tsp45I	GTSAC	1 cut(s)	2229
TspGWI	ACGGA	1 cut(s)	449
TspRI	CASTG	3 cut(s)	666, 1190, 1898
Tth111I	GACNNNGTC	1 cut(s)	2583
Van91I	CCANNNNNTGG	3 cut(s)	32, 284, 2177
VpaK11BI	GGWCC	3 cut(s)	227, 656, 1835
XapI	RAATTY	9 cut(s)	70, 204, 1067, 1079, 1451, 1954, 2095, 2308, 2391
XceI	RCATGY	1 cut(s)	784
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1958, 2714
XhoI	CTCGAG	1 cut(s)	2664
XmnI	GAANNNNTTC	1 cut(s)	2632
XspI	CTAG	3 cut(s)	2022, 2187, 2582
Zsp2I	ATGCAT	1 cut(s)	1561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.