Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004194

Physical Location & Seq

Genomic Coordinates Reverse (-)

9340703 .. 9353438

Gene Structure 12736 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1054400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGGTAGCCAAGGGCATCGCTCGCGTGAAGAAACAGTTCCTTCGCCCTCCACCCATAAAAGCTCTCTCTAACGACCAGAACGACGCCGCAGCGAAATCGGCAGCAGGCATCTGCCCGGAGGTGGCGAAAAGAGGAGATGTTAGCCGGTGCCCGTACAACGATAAATGCCGGTTCAGCCACGACATAGAGGCTTATAAAGCTCAGAAACCTGAGGATATAGAGGGAGAGTGTCCTTTCATGACTGCCCAGGAGACATGCCCATATGGGTTGGCATGTAGGTTCTTGGGTTCGGATAAAGACGGTGTTGAAGGTGGGGATTTGAGTGCTCGCAGAAGAAGCTCTGAGGTGAATGGATTGACCAAAGATGTTCAAAAGCTTTTGTGGAAGAATAAAATGAAGTTCCCCAAAGCAGACGCCAAACTCAAGGCTCTTGGGCTCGAGCGATGTAGGTCAAGCATCAGAATGGGCACTGCTGAGGTGACATTCATCCGAGGATTTGTTTGTGTTCAGATATGTGGATCACATCCAGACACTGTGGCACACACTGTTGACCTAATAGATCAGGAATGTCAAGTGGACTTTATTGATATAAATATGGGGTGCCCAATTGATATTGTTGTGAACAAGGGTGCTGGGTCAGCTCTTCTTACAAAACCCATGCTGATGAAAGGCATTGTACAAGCAGCTTCTGGTACCGTCGACAGTCCTATAACTATTAAGTGTGCCAGAAAGGCACCTGAGGCTTTGCCAGTACTTGGAAATGGAGACATATTTTCATATGTAGATTGGAACAAGCACCAGGCTGAATGCCCTGAGCTTTCTACATGCATGATTGCTCTTTCTACATGCATGATTGCTCGAGGAGCACTAATTAAGCCTTGGAGTTTTACTGAAATAAAGGAGCAGAGGCACTGGGACATAAGTTCTGGGGAGCGATTAAATATATTGAAGGATTACGTACGTCTTGGCCTTGAACATTGGGGCTCTGACACAAAAGTTGGACTGGTATCTATGTCCCAGGTGTGGAGACAGGTTAGGGTCTGTGAGATGTTGCTTGGAAAGGTTCCAGATGTTTTTACATTTGCACCAAAGCACAAATCCAATTCTTACGACCAAGCAGAAAATGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002943 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016491 GO:0016627 GO:0017150 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0055114 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dus zf-CCCH

Protein Analysis

377

Amino Acids

41.4

Weight (kDa)

8.51

Isoelectric Point (pI)

38.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCCH_DUS3L	PF25585	36 - 68	5.7e-11	DUS3L CCCH zinc finger domain
Dus	PF01207	166 - 241	6.8e-18	Dihydrouridine synthase (Dus)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38890
fragaria_vesca	FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690
malus_domestica	MD05G1054400.v1.1 MD10G1062000.v1.1
prunus_persica	Prupe.8G082800_v2.0.a1
pyrus_communis	pycom10g04770
rosa_chinensis	RchiOBHm_Chr6g0268371 RchiOBHm_Chr6g0268451 RchiOBHm_Chr6g0268461 RchiOBHm_Chr6g0271651 RchiOBHm_Chr6g0271661
rosa_laevigata	RLG00000013910
rosa_multiflora	Rmu_sc0002404.1_g000009 Rmu_sc0002404.1_g000017 Rmu_sc0007949.1_g000004 Rmu_sc0008184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00199830
rosa_rugosa	Rorug06G0041000 Rorug06G0042100
rosa_samantha	Rh6AG161000 Rh6BG165400 Rh6CG152700 Rh6CG159400 Rh6DG151200
rosa_wichuraiana	Rw6G013920 Rw6G013960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	195
Acc65I	GGTACC	1 cut(s)	692
AccB1I	GGYRCC	4 cut(s)	147, 600, 692, 733
AccB7I	CCANNNNNTGG	1 cut(s)	755
AccI	GTMKAC	1 cut(s)	699
AccII	CGCG	1 cut(s)	24
AciI	CCGC	1 cut(s)	87
AclWI	GGATC	1 cut(s)	526
AcyI	GRCGYC	2 cut(s)	84, 414
AfaI	GTAC	5 cut(s)	155, 678, 694, 753, 960
AfiI	CCNNNNNNNGG	3 cut(s)	121, 755, 1023
AgsI	TTSAA	4 cut(s)	308, 371, 949, 974
AjnI	CCWGG	3 cut(s)	246, 798, 1017
AluBI	AGCT	7 cut(s)	62, 200, 339, 376, 641, 686, 817
AluI	AGCT	7 cut(s)	62, 200, 339, 376, 641, 686, 817
Alw21I	GWGCWC	2 cut(s)	328, 868
Alw26I	GTCTC	3 cut(s)	245, 759, 1021
AlwI	GGATC	1 cut(s)	526
AlwNI	CAGNNNCTG	2 cut(s)	533, 689
Ama87I	CYCGRG	2 cut(s)	437, 858
AoxI	GGCC	1 cut(s)	967
ApeKI	GCWGC	3 cut(s)	89, 101, 683
Asp700I	GAANNNNTTC	1 cut(s)	35
Asp718I	GGTACC	1 cut(s)	692
AsuC2I	CCSGG	1 cut(s)	116
AsuHPI	GGTGA	2 cut(s)	358, 490
AvaI	CYCGRG	2 cut(s)	437, 858
AxyI	CCTNAGG	2 cut(s)	210, 738
BaeGI	GKGCMC	3 cut(s)	152, 470, 605
BanI	GGYRCC	4 cut(s)	147, 600, 692, 733
BanII	GRGCYC	2 cut(s)	438, 986
Bbv12I	GWGCWC	2 cut(s)	328, 868
BbvCI	CCTCAGC	1 cut(s)	474
BbvI	GCAGC	3 cut(s)	101, 113, 695
BcgI	CGANNNNNNTGC	2 cut(s)	78, 112
BciT130I	CCWGG	3 cut(s)	248, 800, 1019
BcnI	CCSGG	1 cut(s)	116
BcoDI	GTCTC	3 cut(s)	245, 759, 1021
BglI	GCCNNNNNGGC	1 cut(s)	731
BisI	GCNGC	4 cut(s)	87, 90, 102, 684
BlsI	GCNGC	4 cut(s)	88, 91, 103, 685
BmcAI	AGTACT	1 cut(s)	753
Bme1390I	CCNGG	4 cut(s)	116, 248, 800, 1019
BmeT110I	CYCGRG	2 cut(s)	437, 858
BmiI	GGNNCC	5 cut(s)	149, 602, 694, 735, 1065
BmrFI	CCNGG	4 cut(s)	116, 248, 800, 1019
BmrI	ACTGGG	1 cut(s)	922
BmsI	GCATC	3 cut(s)	24, 117, 465
BmuI	ACTGGG	1 cut(s)	922
Bpu10I	CCTNAGC	2 cut(s)	474, 813
BpuEI	CTTGAG	1 cut(s)	407
BpuMI	CCSGG	1 cut(s)	116
BsaAI	YACGTR	1 cut(s)	958
BsaHI	GRCGYC	2 cut(s)	84, 414
BsaJI	CCNNGG	5 cut(s)	9, 246, 490, 878, 1017
Bsc4I	CCNNNNNNNGG	3 cut(s)	121, 755, 1023
Bse118I	RCCGGY	2 cut(s)	144, 168
Bse1I	ACTGG	3 cut(s)	749, 917, 1008
Bse21I	CCTNAGG	2 cut(s)	210, 738
BseBI	CCWGG	3 cut(s)	248, 800, 1019
BseDI	CCNNGG	5 cut(s)	9, 246, 490, 878, 1017
BseGI	GGATG	2 cut(s)	486, 523
BseLI	CCNNNNNNNGG	3 cut(s)	121, 755, 1023
BseMII	CTCAG	6 cut(s)	201, 215, 333, 465, 729, 804
BseNI	ACTGG	3 cut(s)	749, 917, 1008
BseRI	GAGGAG	2 cut(s)	147, 876
BseSI	GKGCMC	3 cut(s)	152, 470, 605
BseXI	GCAGC	3 cut(s)	101, 113, 695
BseYI	CCCAGC	1 cut(s)	632
Bsh1236I	CGCG	1 cut(s)	24
BshFI	GGCC	1 cut(s)	969
BshNI	GGYRCC	4 cut(s)	147, 600, 692, 733
BsiHKAI	GWGCWC	2 cut(s)	328, 868
BsiHKCI	CYCGRG	2 cut(s)	437, 858
BsiSI	CCGG	3 cut(s)	116, 145, 169
BsiWI	CGTACG	1 cut(s)	958
BslFI	GGGAC	2 cut(s)	929, 1000
BslI	CCNNNNNNNGG	3 cut(s)	121, 755, 1023
BsmAI	GTCTC	3 cut(s)	245, 759, 1021
BsmFI	GGGAC	2 cut(s)	929, 1000
BsmI	GAATGC	1 cut(s)	812
BsnI	GGCC	1 cut(s)	969
BsoBI	CYCGRG	2 cut(s)	437, 858
Bsp1286I	GDGCHC	7 cut(s)	152, 328, 438, 470, 605, 868, 986
Bsp1407I	TGTACA	1 cut(s)	676
Bsp143I	GATC	2 cut(s)	518, 559
BspACI	CCGC	1 cut(s)	87
BspANI	GGCC	1 cut(s)	969
BspCNI	CTCAG	6 cut(s)	202, 214, 334, 466, 730, 805
BspFNI	CGCG	1 cut(s)	24
BspHI	TCATGA	1 cut(s)	237
BspLI	GGNNCC	5 cut(s)	149, 602, 694, 735, 1065
BspPI	GGATC	1 cut(s)	526
BspQI	GCTCTTC	1 cut(s)	648
BspT107I	GGYRCC	4 cut(s)	147, 600, 692, 733
BsrFI	RCCGGY	2 cut(s)	144, 168
BsrGI	TGTACA	1 cut(s)	676
BsrI	ACTGG	3 cut(s)	749, 917, 1008
BssAI	RCCGGY	2 cut(s)	144, 168
BssECI	CCNNGG	5 cut(s)	9, 246, 490, 878, 1017
BssMI	GATC	2 cut(s)	518, 559
BssNI	GRCGYC	2 cut(s)	84, 414
BssT1I	CCWWGG	2 cut(s)	9, 878
Bst2UI	CCWGG	3 cut(s)	248, 800, 1019
Bst4CI	ACNGT	6 cut(s)	36, 302, 535, 547, 697, 704
Bst6I	CTCTTC	1 cut(s)	648
BstACI	GRCGYC	2 cut(s)	84, 414
BstAUI	TGTACA	1 cut(s)	676
BstBAI	YACGTR	1 cut(s)	958
BstC8I	GCNNGC	3 cut(s)	22, 106, 328
BstDEI	CTNAG	6 cut(s)	201, 210, 342, 474, 738, 813
BstF5I	GGATG	2 cut(s)	486, 523
BstFNI	CGCG	1 cut(s)	24
BstKTI	GATC	2 cut(s)	521, 562
BstMAI	GTCTC	3 cut(s)	245, 759, 1021
BstMBI	GATC	2 cut(s)	518, 559
BstMWI	GCNNNNNNNGC	9 cut(s)	21, 98, 174, 197, 336, 638, 731, 740, 863
BstNI	CCWGG	3 cut(s)	248, 800, 1019
BstNSI	RCATGY	4 cut(s)	258, 276, 828, 849
BstSCI	CCNGG	4 cut(s)	114, 246, 798, 1017
BstSLI	GKGCMC	3 cut(s)	152, 470, 605
BstSNI	TACGTA	1 cut(s)	958
BstUI	CGCG	1 cut(s)	24
BstV1I	GCAGC	3 cut(s)	101, 113, 695
Bsu36I	CCTNAGG	2 cut(s)	210, 738
BsuRI	GGCC	1 cut(s)	969
BtgZI	GCGATG	1 cut(s)	457
BtsCI	GGATG	2 cut(s)	486, 523
BtsI	GCAGTG	1 cut(s)	468
BtsIMutI	CAGTG	4 cut(s)	468, 531, 543, 910
Cac8I	GCNNGC	3 cut(s)	22, 106, 328
CaiI	CAGNNNCTG	2 cut(s)	533, 689
CciI	TCATGA	1 cut(s)	237
Cfr10I	RCCGGY	2 cut(s)	144, 168
CseI	GACGC	2 cut(s)	92, 422
Csp6I	GTAC	5 cut(s)	154, 677, 693, 752, 959
CviAII	CATG	8 cut(s)	238, 255, 273, 658, 825, 829, 846, 850
CviQI	GTAC	5 cut(s)	154, 677, 693, 752, 959
DdeI	CTNAG	6 cut(s)	201, 210, 342, 474, 738, 813
DpnI	GATC	2 cut(s)	520, 561
DpnII	GATC	2 cut(s)	518, 559
Eam1104I	CTCTTC	1 cut(s)	648
EarI	CTCTTC	1 cut(s)	648
Eco105I	TACGTA	1 cut(s)	958
Eco130I	CCWWGG	2 cut(s)	9, 878
Eco24I	GRGCYC	2 cut(s)	438, 986
Eco81I	CCTNAGG	2 cut(s)	210, 738
Eco88I	CYCGRG	2 cut(s)	437, 858
EcoRII	CCWGG	3 cut(s)	246, 798, 1017
EcoT14I	CCWWGG	2 cut(s)	9, 878
EcoT22I	ATGCAT	2 cut(s)	830, 851
EcoT38I	GRGCYC	2 cut(s)	438, 986
ErhI	CCWWGG	2 cut(s)	9, 878
FaeI	CATG	8 cut(s)	241, 258, 276, 661, 828, 832, 849, 853
FaqI	GGGAC	2 cut(s)	929, 1000
FatI	CATG	8 cut(s)	237, 254, 272, 657, 824, 828, 845, 849
FauNDI	CATATG	2 cut(s)	262, 778
FblI	GTMKAC	1 cut(s)	699
Fnu4HI	GCNGC	4 cut(s)	87, 90, 102, 684
FokI	GGATG	2 cut(s)	473, 510
FriOI	GRGCYC	2 cut(s)	438, 986
Fsp4HI	GCNGC	4 cut(s)	87, 90, 102, 684
GluI	GCNGC	4 cut(s)	87, 90, 102, 684
GsaI	CCCAGC	1 cut(s)	636
HaeIII	GGCC	1 cut(s)	969
HapII	CCGG	3 cut(s)	116, 145, 169
HgaI	GACGC	2 cut(s)	92, 422
Hin1I	GRCGYC	2 cut(s)	84, 414
Hin1II	CATG	8 cut(s)	241, 258, 276, 661, 828, 832, 849, 853
HincII	GTYRAC	2 cut(s)	550, 700
HindII	GTYRAC	2 cut(s)	550, 700
HindIII	AAGCTT	1 cut(s)	374
HpaII	CCGG	3 cut(s)	116, 145, 169
HphI	GGTGA	2 cut(s)	358, 490
Hpy166II	GTNNAC	4 cut(s)	550, 577, 622, 700
Hpy188I	TCNGA	7 cut(s)	204, 292, 343, 461, 491, 510, 988
Hpy188III	TCNNGA	4 cut(s)	238, 527, 563, 1067
Hpy8I	GTNNAC	4 cut(s)	550, 577, 622, 700
Hpy99I	CGWCG	2 cut(s)	86, 701
HpyAV	CCTTC	3 cut(s)	50, 302, 943
HpyCH4III	ACNGT	6 cut(s)	36, 302, 535, 547, 697, 704
HpyCH4IV	ACGT	2 cut(s)	957, 961
HpyCH4V	TGCA	3 cut(s)	828, 849, 1085
HpyF10VI	GCNNNNNNNGC	9 cut(s)	21, 98, 174, 197, 336, 638, 731, 740, 863
HpyF3I	CTNAG	6 cut(s)	201, 210, 342, 474, 738, 813
HpySE526I	ACGT	2 cut(s)	957, 961
Hsp92I	GRCGYC	2 cut(s)	84, 414
Hsp92II	CATG	8 cut(s)	241, 258, 276, 661, 828, 832, 849, 853
KpnI	GGTACC	1 cut(s)	696
Kzo9I	GATC	2 cut(s)	518, 559
LguI	GCTCTTC	1 cut(s)	648
LmnI	GCTCC	3 cut(s)	863, 901, 931
Lsp1109I	GCAGC	3 cut(s)	101, 113, 695
LweI	GCATC	3 cut(s)	24, 117, 465
MaeII	ACGT	2 cut(s)	957, 961
MaeIII	GTNAC	1 cut(s)	478
MalI	GATC	2 cut(s)	520, 561
MboI	GATC	2 cut(s)	518, 559
MboII	GAAGA	4 cut(s)	40, 345, 397, 635
MfeI	CAATTG	1 cut(s)	606
MhlI	GDGCHC	7 cut(s)	152, 328, 438, 470, 605, 868, 986
MluCI	AATT	3 cut(s)	606, 870, 1102
MmeI	TCCRAC	1 cut(s)	979
Mph1103I	ATGCAT	2 cut(s)	830, 851
MroXI	GAANNNNTTC	1 cut(s)	35
MseI	TTAA	3 cut(s)	717, 873, 938
MslI	CAYNNNNRTG	2 cut(s)	461, 662
MspI	CCGG	3 cut(s)	116, 145, 169
MspR9I	CCNGG	4 cut(s)	116, 248, 800, 1019
MunI	CAATTG	1 cut(s)	606
Mva1269I	GAATGC	1 cut(s)	812
MvaI	CCWGG	3 cut(s)	248, 800, 1019
MvnI	CGCG	1 cut(s)	24
MwoI	GCNNNNNNNGC	9 cut(s)	21, 98, 174, 197, 336, 638, 731, 740, 863
NciI	CCSGG	1 cut(s)	116
NdeI	CATATG	2 cut(s)	262, 778
NdeII	GATC	2 cut(s)	518, 559
NlaIII	CATG	8 cut(s)	241, 258, 276, 661, 828, 832, 849, 853
NlaIV	GGNNCC	5 cut(s)	149, 602, 694, 735, 1065
NmuCI	GTSAC	1 cut(s)	478
NsiI	ATGCAT	2 cut(s)	830, 851
NspI	RCATGY	4 cut(s)	258, 276, 828, 849
PaeR7I	CTCGAG	2 cut(s)	437, 858
PagI	TCATGA	1 cut(s)	237
PciSI	GCTCTTC	1 cut(s)	648
PcsI	WCGNNNNNNNCGW	1 cut(s)	78
PctI	GAATGC	1 cut(s)	812
PdmI	GAANNNNTTC	1 cut(s)	35
Pfl23II	CGTACG	1 cut(s)	958
PflMI	CCANNNNNTGG	1 cut(s)	755
PkrI	GCNGC	4 cut(s)	88, 91, 103, 685
Ppu21I	YACGTR	1 cut(s)	958
PsiI	TTATAA	1 cut(s)	195
Psp6I	CCWGG	3 cut(s)	246, 798, 1017
PspFI	CCCAGC	1 cut(s)	632
PspGI	CCWGG	3 cut(s)	246, 798, 1017
PspLI	CGTACG	1 cut(s)	958
PspN4I	GGNNCC	5 cut(s)	149, 602, 694, 735, 1065
PspXI	VCTCGAGB	2 cut(s)	437, 858
PstNI	CAGNNNCTG	2 cut(s)	533, 689
RsaI	GTAC	5 cut(s)	155, 678, 694, 753, 960
RsaNI	GTAC	5 cut(s)	154, 677, 693, 752, 959
RseI	CAYNNNNRTG	2 cut(s)	461, 662
SalI	GTCGAC	1 cut(s)	698
SapI	GCTCTTC	1 cut(s)	648
SaqAI	TTAA	3 cut(s)	717, 873, 938
SatI	GCNGC	4 cut(s)	87, 90, 102, 684
Sau3AI	GATC	2 cut(s)	518, 559
ScaI	AGTACT	1 cut(s)	753
ScrFI	CCNGG	4 cut(s)	116, 248, 800, 1019
SduI	GDGCHC	7 cut(s)	152, 328, 438, 470, 605, 868, 986
SfaNI	GCATC	3 cut(s)	24, 117, 465
Sfr274I	CTCGAG	2 cut(s)	437, 858
SlaI	CTCGAG	2 cut(s)	437, 858
SmiMI	CAYNNNNRTG	2 cut(s)	461, 662
SmlI	CTYRAG	3 cut(s)	422, 437, 858
SmoI	CTYRAG	3 cut(s)	422, 437, 858
SnaBI	TACGTA	1 cut(s)	958
Sse9I	AATT	3 cut(s)	606, 870, 1102
SsiI	CCGC	1 cut(s)	87
StyD4I	CCNGG	4 cut(s)	114, 246, 798, 1017
StyI	CCWWGG	2 cut(s)	9, 878
TaaI	ACNGT	6 cut(s)	36, 302, 535, 547, 697, 704
TaiI	ACGT	2 cut(s)	960, 964
TaqI	TCGA	3 cut(s)	438, 699, 859
TasI	AATT	3 cut(s)	606, 870, 1102
TatI	WGTACW	2 cut(s)	676, 751
TauI	GCSGC	1 cut(s)	89
Tru1I	TTAA	3 cut(s)	717, 873, 938
Tru9I	TTAA	3 cut(s)	717, 873, 938
TscAI	CASTG	4 cut(s)	475, 538, 550, 917
TseFI	GTSAC	1 cut(s)	478
TseI	GCWGC	3 cut(s)	89, 101, 683
Tsp45I	GTSAC	1 cut(s)	478
TspDTI	ATGAA	5 cut(s)	226, 410, 475, 680, 765
TspRI	CASTG	4 cut(s)	475, 538, 550, 917
Van91I	CCANNNNNTGG	1 cut(s)	755
XceI	RCATGY	4 cut(s)	258, 276, 828, 849
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1121
XhoI	CTCGAG	2 cut(s)	437, 858
XmiI	GTMKAC	1 cut(s)	699
XmnI	GAANNNNTTC	1 cut(s)	35
ZrmI	AGTACT	1 cut(s)	753
Zsp2I	ATGCAT	2 cut(s)	830, 851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.