Rh6BG165400

tRNA-dihydrouridine(47) synthase NAD(P)( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004194
Physical Location & Seq
Forward (+)
26787115 .. 26794461
7347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG165400.1

Sequence Viewer

Length: 1722 bp
ATGGCTATCGATGAGCCATGCCCTTATGGTTTGTCATGTAGGTTCTTGGGTACACATAAAAATGGTGTTGCAGATGGAGATGTGAATGCTCGTAGAAGAAGCTCTGAGATGAATGGATTAAGCAAAAATGTTCAAAAGCTTTTGTGGAAAAATAAAATGAAGTTCACCAAGGCTGATACCAAACTTAAGGCTCTTGGTCTCATGGGGCATGCCAATTCAAAAATAATTGCCATGTCAAAGGAGAATGGGTCGGTGGTTTCAAAGAATTGTGATGTTACTGATGGAAATGGCTGTAGTGAATTTCCCAGTAACACAGATGATAAAGTGGAGTTGACTGCTGAAACAAACGAAGAGGGACGTGTTGATGAAAAGTTTCCATCTGATGAAATGGAGTTGTCTGCTGAAGTGAAAGAAGAGGGAGGTGCTAATGAAACATTTCCATCTGACGATTCTAGGCCTTCAAAGAAGTCAAGATCTGTAATTGAAGAGGAGTTTGGTTCTGGTGAAGCAGATTATGGAGGTGTGAGAGCTGCAGGAAAAGTTGTTGGGCAAACCTGCACGGAACCTGAACCAGAAGCTGCAGCTAACATTGAATTCTCAGAGAATGATAGAAGTTTAAAGACACACCCACGTGAAAAGAAGCTTATTGACTTCAGAGAAAAGTTGTACCTTGCACCTCTGACCACAGTCGGAAATCTTCCATTTCGAAGGGTTTGCAAAGGTTTAGGGGCAGATATAACATGTGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCGTCGGAATGGGCCTTGCTGAGGAGACATTCATCTGAAGATTTGTTTGGCGTTCAGATATGTGGGGCATATCCAGACACCGTAGCACGCACTGTTGAACTTATAGAACAGGAATGTACCGTAGACTTCATTGATATAAATATGGGGTGCCCAATAGATATTGTTGTGAACAAGGGTGCTGGCTCAGCTCTTCTAACAAAACCAATGCGAATGAAAGGCATTGTGCAAGCAGCTTCTGGTACAGTTGACATTCCTATGACTATCAAGGTCCGCACAGCCTATTTTGAGGGGAAAAATCGCATTGACTCGCTTATTGCTGACATGGGCGACTGGGGAGCCAGTGCAGTAACAATTCATGGTCGATCACGACAGCAACGCTACAGCAAGCTTGCAGATTGGGAATATGTATACCAGTGTGCTAAAAAAGCCCCTGATTCTTTGCAAGTCCTTGGAAATGGAGATGTATTTTCGTATGTAGATTGGAACAAGCACAAGGCTGAGTGCCCTGAACTGTCTTCATGCATGATTGCTCGAGGAGCACTAATTAAGCCTTGGATTTTTACAGAAATAAAGGAGCAGAGGCACTGGGACATCACTTCTGGGGAGCGATTTAATATTTTGAAGGATTATGTACGTTGTGGCCTTGAACATTGGGGCTCTGACACAAAAGGTGTCGAGACAACTAGGCGTTTTCTGTTAGAATGGCTTAGCTACACTTGTAGATATGTACCTGTTGGTCTTCTAGATGTCATCCCACAAAGACTCAGCTGGCGCCCACCATCTTATTTTGGACGTGATGACCTTGAGACACTAATGGCTTCTGATTCTGCTGCTGACTGGGTTAGGCTCTCTGAGATGTTGCTTGGGAAGGTTCCAGATGGTTTCACATTTGCACCAAAGCATAAATCCAATGCTTACGACCGAGCAGAAAACGGCTAA

Protein Analysis

573

Amino Acids

63.62

Weight (kDa)

5.63

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 224 - 478 7.4e-49 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 930, 1554
AccI GTMKAC 2 cut(s) 906, 1191
AciI CCGC 1 cut(s) 1054
AcsI RAATTY 2 cut(s) 299, 593
AcuI CTGAAG 3 cut(s) 423, 637, 840
AcvI CACGTG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 1555
AfaI GTAC 7 cut(s) 52, 668, 763, 901, 1024, 1416, 1512
AfiI CCNNNNNNNGG 1 cut(s) 804
AflII CTTAAG 1 cut(s) 185
AflIII ACRYGT 2 cut(s) 358, 740
AgsI TTSAA 9 cut(s) 134, 219, 261, 462, 485, 593, 881, 1405, 1430
AjiI CACGTC 2 cut(s) 359, 1577
AjuI GAANNNNNNNTTGG 4 cut(s) 477, 509, 813, 845
AleI CACNNNNGTG 1 cut(s) 630
Alw21I GWGCWC 1 cut(s) 1324
Alw26I GTCTC 4 cut(s) 203, 802, 1454, 1583
AlwNI CAGNNNCTG 2 cut(s) 578, 1019
Ama87I CYCGRG 1 cut(s) 1314
AoxI GGCC 3 cut(s) 455, 795, 1423
ApeKI GCWGC 5 cut(s) 530, 578, 581, 1013, 1613
ApoI RAATTY 2 cut(s) 299, 593
Asp700I GAANNNNTTC 2 cut(s) 372, 435
AspLEI GCGC 1 cut(s) 1557
AspS9I GGNCC 2 cut(s) 795, 1051
AsuHPI GGTGA 3 cut(s) 157, 515, 758
AsuII TTCGAA 1 cut(s) 706
AvaI CYCGRG 1 cut(s) 1314
AvaII GGWCC 1 cut(s) 1051
BaeGI GKGCMC 2 cut(s) 935, 1289
BanI GGYRCC 2 cut(s) 930, 1554
BanII GRGCYC 1 cut(s) 1442
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 1290, 1514
Bbv12I GWGCWC 1 cut(s) 1324
BbvCI CCTCAGC 1 cut(s) 803
BbvI GCAGC 5 cut(s) 517, 565, 593, 1025, 1600
BccI CCATC 6 cut(s) 68, 275, 385, 448, 1570, 1655
BcgI CGANNNNNNTGC 2 cut(s) 696, 730
BcoDI GTCTC 4 cut(s) 203, 802, 1454, 1583
BfaI CTAG 3 cut(s) 453, 1467, 1526
BfmI CTRYAG 4 cut(s) 292, 531, 579, 1162
BfoI RGCGCY 1 cut(s) 1558
BfrI CTTAAG 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 563
BglII AGATCT 1 cut(s) 473
BisI GCNGC 5 cut(s) 531, 579, 582, 1014, 1614
BlpI GCTNAGC 2 cut(s) 967, 1490
BlsI GCNGC 5 cut(s) 532, 580, 583, 1015, 1615
Bme18I GGWCC 1 cut(s) 1051
BmeT110I CYCGRG 1 cut(s) 1314
BmgBI CACGTC 2 cut(s) 359, 1577
BmgT120I GGNCC 2 cut(s) 795, 1051
BmiI GGNNCC 5 cut(s) 564, 932, 1120, 1556, 1656
BmrI ACTGGG 4 cut(s) 300, 1123, 1378, 1630
BmuI ACTGGG 4 cut(s) 300, 1123, 1378, 1630
BoxI GACNNNNGTC 1 cut(s) 686
BpiI GAAGAC 2 cut(s) 1290, 1514
Bpu10I CCTNAGC 1 cut(s) 803
Bpu1102I GCTNAGC 2 cut(s) 967, 1490
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 1 cut(s) 1607
Bsa29I ATCGAT 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 632
BsaHI GRCGYC 1 cut(s) 1555
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 168, 1231, 1334
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 804
Bse1I ACTGG 6 cut(s) 306, 1118, 1122, 1195, 1373, 1625
Bse3DI GCAATG 1 cut(s) 762
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 3 cut(s) 168, 1231, 1334
BseGI GGATG 1 cut(s) 1533
BseLI CCNNNNNNNGG 1 cut(s) 804
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 7 cut(s) 96, 612, 794, 981, 1272, 1561, 1626
BseNI ACTGG 6 cut(s) 306, 1118, 1122, 1195, 1373, 1625
BseRI GAGGAG 3 cut(s) 503, 820, 1332
BseSI GKGCMC 2 cut(s) 935, 1289
BseXI GCAGC 5 cut(s) 517, 565, 593, 1025, 1600
BsgI GTGCAG 2 cut(s) 541, 1146
Bsh1285I CGRYCG 1 cut(s) 1705
BshFI GGCC 3 cut(s) 457, 797, 1425
BshNI GGYRCC 2 cut(s) 930, 1554
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 1705
BsiHKAI GWGCWC 1 cut(s) 1324
BsiHKCI CYCGRG 1 cut(s) 1314
BslFI GGGAC 2 cut(s) 369, 1385
BslI CCNNNNNNNGG 1 cut(s) 804
BsmAI GTCTC 4 cut(s) 203, 802, 1454, 1583
BsmFI GGGAC 2 cut(s) 369, 1385
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 3 cut(s) 457, 797, 1425
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 1314
Bsp119I TTCGAA 1 cut(s) 706
Bsp1286I GDGCHC 4 cut(s) 935, 1289, 1324, 1442
Bsp1407I TGTACA 1 cut(s) 761
Bsp143I GATC 2 cut(s) 473, 1145
Bsp1720I GCTNAGC 2 cut(s) 967, 1490
BspACI CCGC 1 cut(s) 1054
BspANI GGCC 3 cut(s) 457, 797, 1425
BspCNI CTCAG 7 cut(s) 97, 611, 795, 980, 1273, 1560, 1627
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 5 cut(s) 564, 932, 1120, 1556, 1656
BspMAI CTGCAG 2 cut(s) 535, 583
BspMI ACCTGC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 978
BspT104I TTCGAA 1 cut(s) 706
BspT107I GGYRCC 2 cut(s) 930, 1554
BspTI CTTAAG 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 762
BsrGI TGTACA 1 cut(s) 761
BsrI ACTGG 6 cut(s) 306, 1118, 1122, 1195, 1373, 1625
BssECI CCNNGG 3 cut(s) 168, 1231, 1334
BssMI GATC 2 cut(s) 473, 1145
BssNAI GTATAC 1 cut(s) 1192
BssNI GRCGYC 1 cut(s) 1555
BssT1I CCWWGG 3 cut(s) 168, 1231, 1334
Bst1107I GTATAC 1 cut(s) 1192
Bst4CI ACNGT 6 cut(s) 688, 865, 877, 904, 1027, 1296
Bst6I CTCTTC 4 cut(s) 345, 408, 480, 978
BstACI GRCGYC 1 cut(s) 1555
BstAFI CTTAAG 1 cut(s) 185
BstAUI TGTACA 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 706
BstC8I GCNNGC 7 cut(s) 210, 871, 964, 1011, 1169, 1173, 1553
BstDEI CTNAG 8 cut(s) 105, 598, 803, 967, 1281, 1490, 1547, 1635
BstENI CCTNNNNNAGG 1 cut(s) 802
BstF5I GGATG 1 cut(s) 1533
BstH2I RGCGCY 1 cut(s) 1558
BstHHI GCGC 1 cut(s) 1557
BstKTI GATC 2 cut(s) 476, 1148
BstMAI GTCTC 4 cut(s) 203, 802, 1454, 1583
BstMBI GATC 2 cut(s) 473, 1145
BstMCI CGRYCG 1 cut(s) 1705
BstMWI GCNNNNNNNGC 3 cut(s) 968, 1208, 1319
BstNSI RCATGY 2 cut(s) 212, 744
BstPAI GACNNNNGTC 1 cut(s) 686
BstSFI CTRYAG 4 cut(s) 292, 531, 579, 1162
BstSLI GKGCMC 2 cut(s) 935, 1289
BstV1I GCAGC 5 cut(s) 517, 565, 593, 1025, 1600
BstV2I GAAGAC 2 cut(s) 1290, 1514
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BstZ17I GTATAC 1 cut(s) 1192
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 3 cut(s) 457, 797, 1425
BsuTUI ATCGAT 1 cut(s) 9
BtrI CACGTC 2 cut(s) 359, 1577
BtsCI GGATG 1 cut(s) 1533
BtsIMutI CAGTG 4 cut(s) 873, 1129, 1202, 1366
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 7 cut(s) 210, 871, 964, 1011, 1169, 1173, 1553
CaiI CAGNNNCTG 2 cut(s) 578, 1019
CfoI GCGC 1 cut(s) 1557
Cfr13I GGNCC 2 cut(s) 795, 1051
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 1 cut(s) 774
Csp6I GTAC 7 cut(s) 51, 667, 762, 900, 1023, 1415, 1511
CviQI GTAC 7 cut(s) 51, 667, 762, 900, 1023, 1415, 1511
DdeI CTNAG 8 cut(s) 105, 598, 803, 967, 1281, 1490, 1547, 1635
DinI GGCGCC 1 cut(s) 1556
DpnI GATC 2 cut(s) 475, 1147
DpnII GATC 2 cut(s) 473, 1145
DraI TTTAAA 1 cut(s) 618
Eam1104I CTCTTC 4 cut(s) 345, 408, 480, 978
EarI CTCTTC 4 cut(s) 345, 408, 480, 978
Eco130I CCWWGG 3 cut(s) 168, 1231, 1334
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 1442
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 1051
Eco57I CTGAAG 3 cut(s) 423, 637, 840
Eco72I CACGTG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 1314
EcoNI CCTNNNNNAGG 1 cut(s) 802
EcoRI GAATTC 1 cut(s) 593
EcoT14I CCWWGG 3 cut(s) 168, 1231, 1334
EcoT22I ATGCAT 1 cut(s) 1307
EcoT38I GRGCYC 1 cut(s) 1442
EgeI GGCGCC 1 cut(s) 1556
EheI GGCGCC 1 cut(s) 1556
ErhI CCWWGG 3 cut(s) 168, 1231, 1334
FaqI GGGAC 2 cut(s) 369, 1385
FblI GTMKAC 2 cut(s) 906, 1191
Fnu4HI GCNGC 5 cut(s) 531, 579, 582, 1014, 1614
FokI GGATG 1 cut(s) 1520
FriOI GRGCYC 1 cut(s) 1442
Fsp4HI GCNGC 5 cut(s) 531, 579, 582, 1014, 1614
FspBI CTAG 3 cut(s) 453, 1467, 1526
GlaI GCGC 1 cut(s) 1556
GluI GCNGC 5 cut(s) 531, 579, 582, 1014, 1614
HaeII RGCGCY 1 cut(s) 1558
HaeIII GGCC 3 cut(s) 457, 797, 1425
HgaI GACGC 1 cut(s) 774
HhaI GCGC 1 cut(s) 1557
Hin1I GRCGYC 1 cut(s) 1555
Hin6I GCGC 1 cut(s) 1555
HinP1I GCGC 1 cut(s) 1555
HincII GTYRAC 2 cut(s) 333, 1030
HindII GTYRAC 2 cut(s) 333, 1030
HindIII AAGCTT 3 cut(s) 137, 641, 1169
HinfI GANTC 5 cut(s) 449, 1088, 1217, 1545, 1607
HphI GGTGA 3 cut(s) 157, 515, 758
Hpy166II GTNNAC 8 cut(s) 53, 165, 333, 762, 907, 952, 1030, 1192
Hpy188III TCNNGA 6 cut(s) 471, 857, 1149, 1459, 1526, 1658
Hpy8I GTNNAC 8 cut(s) 53, 165, 333, 762, 907, 952, 1030, 1192
Hpy99I CGWCG 1 cut(s) 790
HpyAV CCTTC 4 cut(s) 468, 702, 1399, 1645
HpyCH4III ACNGT 6 cut(s) 688, 865, 877, 904, 1027, 1296
HpyCH4IV ACGT 4 cut(s) 358, 631, 1417, 1576
HpyF10VI GCNNNNNNNGC 3 cut(s) 968, 1208, 1319
HpyF3I CTNAG 8 cut(s) 105, 598, 803, 967, 1281, 1490, 1547, 1635
HpySE526I ACGT 4 cut(s) 358, 631, 1417, 1576
Hsp92I GRCGYC 1 cut(s) 1555
HspAI GCGC 1 cut(s) 1555
KasI GGCGCC 1 cut(s) 1554
Kzo9I GATC 2 cut(s) 473, 1145
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 4 cut(s) 1118, 1319, 1357, 1387
Lsp1109I GCAGC 5 cut(s) 517, 565, 593, 1025, 1600
MaeI CTAG 3 cut(s) 453, 1467, 1526
MaeII ACGT 4 cut(s) 358, 631, 1417, 1576
MaeIII GTNAC 3 cut(s) 274, 308, 1129
MalI GATC 2 cut(s) 475, 1147
MboI GATC 2 cut(s) 473, 1145
MboII GAAGA 9 cut(s) 108, 362, 425, 497, 689, 833, 965, 1290, 1514
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 4 cut(s) 935, 1289, 1324, 1442
MluCI AATT 8 cut(s) 214, 225, 265, 299, 480, 593, 1134, 1326
Mly113I GGCGCC 1 cut(s) 1555
MlyI GAGTC 2 cut(s) 1082, 1539
MmeI TCCRAC 2 cut(s) 670, 768
Mph1103I ATGCAT 1 cut(s) 1307
MroXI GAANNNNTTC 2 cut(s) 372, 435
MseI TTAA 5 cut(s) 119, 186, 617, 1329, 1395
MslI CAYNNNNRTG 3 cut(s) 60, 630, 1037
MspA1I CMGCKG 1 cut(s) 1551
MspCI CTTAAG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 91
MwoI GCNNNNNNNGC 3 cut(s) 968, 1208, 1319
NarI GGCGCC 1 cut(s) 1555
NdeII GATC 2 cut(s) 473, 1145
NlaIV GGNNCC 5 cut(s) 564, 932, 1120, 1556, 1656
NsiI ATGCAT 1 cut(s) 1307
NspI RCATGY 2 cut(s) 212, 744
NspV TTCGAA 1 cut(s) 706
OliI CACNNNNGTG 1 cut(s) 630
PaeI GCATGC 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 1314
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 978
PctI GAATGC 1 cut(s) 91
PdmI GAANNNNTTC 2 cut(s) 372, 435
PfeI GAWTC 3 cut(s) 449, 1217, 1607
PkrI GCNGC 5 cut(s) 532, 580, 583, 1015, 1615
PleI GAGTC 2 cut(s) 1082, 1539
PluTI GGCGCC 1 cut(s) 1558
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 2 cut(s) 1082, 1539
Ppu21I YACGTR 1 cut(s) 632
PscI ACATGT 1 cut(s) 740
PshAI GACNNNNGTC 1 cut(s) 686
PspCI CACGTG 1 cut(s) 632
PspN4I GGNNCC 5 cut(s) 564, 932, 1120, 1556, 1656
PspPI GGNCC 2 cut(s) 795, 1051
PspXI VCTCGAGB 1 cut(s) 1314
PstI CTGCAG 2 cut(s) 535, 583
PstNI CAGNNNCTG 2 cut(s) 578, 1019
PsuI RGATCY 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 1551
RsaI GTAC 7 cut(s) 52, 668, 763, 901, 1024, 1416, 1512
RsaNI GTAC 7 cut(s) 51, 667, 762, 900, 1023, 1415, 1511
RseI CAYNNNNRTG 3 cut(s) 60, 630, 1037
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 5 cut(s) 119, 186, 617, 1329, 1395
SatI GCNGC 5 cut(s) 531, 579, 582, 1014, 1614
Sau3AI GATC 2 cut(s) 473, 1145
Sau96I GGNCC 2 cut(s) 795, 1051
SchI GAGTC 2 cut(s) 1082, 1539
SduI GDGCHC 4 cut(s) 935, 1289, 1324, 1442
SfcI CTRYAG 4 cut(s) 292, 531, 579, 1162
SfoI GGCGCC 1 cut(s) 1556
Sfr274I CTCGAG 1 cut(s) 1314
SfuI TTCGAA 1 cut(s) 706
SinI GGWCC 1 cut(s) 1051
SlaI CTCGAG 1 cut(s) 1314
SmiMI CAYNNNNRTG 3 cut(s) 60, 630, 1037
SmlI CTYRAG 3 cut(s) 185, 1314, 1586
SmoI CTYRAG 3 cut(s) 185, 1314, 1586
SphI GCATGC 1 cut(s) 212
Sse9I AATT 8 cut(s) 214, 225, 265, 299, 480, 593, 1134, 1326
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 1 cut(s) 1054
SspDI GGCGCC 1 cut(s) 1554
SspI AATATT 1 cut(s) 1399
SspMI CTAG 3 cut(s) 453, 1467, 1526
StuI AGGCCT 1 cut(s) 457
StyI CCWWGG 3 cut(s) 168, 1231, 1334
TaaI ACNGT 6 cut(s) 688, 865, 877, 904, 1027, 1296
TaiI ACGT 4 cut(s) 361, 634, 1420, 1579
TaqI TCGA 5 cut(s) 9, 706, 1144, 1315, 1458
TaqII GACCGA 1 cut(s) 1719
TasI AATT 8 cut(s) 214, 225, 265, 299, 480, 593, 1134, 1326
TatI WGTACW 1 cut(s) 761
TfiI GAWTC 3 cut(s) 449, 1217, 1607
Tru1I TTAA 5 cut(s) 119, 186, 617, 1329, 1395
Tru9I TTAA 5 cut(s) 119, 186, 617, 1329, 1395
TscAI CASTG 4 cut(s) 880, 1129, 1202, 1373
TseI GCWGC 5 cut(s) 530, 578, 581, 1013, 1613
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 4 cut(s) 880, 1129, 1202, 1373
Vha464I CTTAAG 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 1051
XagI CCTNNNNNAGG 1 cut(s) 802
XapI RAATTY 2 cut(s) 299, 593
XbaI TCTAGA 1 cut(s) 1525
XceI RCATGY 2 cut(s) 212, 744
XhoI CTCGAG 1 cut(s) 1314
XmiI GTMKAC 2 cut(s) 906, 1191
XmnI GAANNNNTTC 2 cut(s) 372, 435
XspI CTAG 3 cut(s) 453, 1467, 1526
Zsp2I ATGCAT 1 cut(s) 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.