Rh6CG159400

tRNA-dihydrouridine(47) synthase NAD(P)( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004194
Physical Location & Seq
Forward (+)
20679176 .. 20681566
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG159400.1

Sequence Viewer

Length: 837 bp
ATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCTTCGGAATGGGCCCTGCTGAGGAGACATTCATCTGAGGATTTGTTTGGCGTTCAGATATGTCGGGCATATCCAGACACCGTAGCATGCACTGTTGAACTTATAGAACAGGAATGTACCGTAGACTTCATTGATATAAATATGGGGTGCCCAATAGATATTGTTGTGAACAAGGGTGCTGGCTCAGCTGTTCTAACAAAACCAATGCGAATGAAAGGCATTGTGCAAGCAGCTTCTGGTACAGTTGACATTCCTATAACTATCAAGGTCCGCACAGCCTATTTTGAGGGGAAAAATCGCATTGACTCACTTATTGCTGACATGGGTAACTGGGGAGCCAGTGCAATTGGAACAAGCACAAGGCTGAGTGCCCTGAACTGTTCATTCATGATTGCTCGAGGAGCACTAATTAAGCCTGGGGTTTTTACAGAAATAAAGGAGCAGAGTTACTGGGACGTCACTTCTGGGGAGCGATTTAATATTTTGAAGGATTATGTACGTTGTGGCCTTGAACATTGGGGCTCTGACACAAAAGGTGTTGAGACAACTAGGCTTTTTCTGTTAGAATGGCTTAGCTACACTTGTAGATATGTACCTGTTGGTCTTCTAGATGTCATCCCACAACGACTGAGCTGGCGCCCACCATCTTATGTTGTACGTGATGACCTTGAGACACTAATGGCTTCTGATTCTGCTGCTGACTGGGTTAGGCTCTCTGAGATGTTGCTTGGGAAGGTTCCAGATGGTTTCACATTTGCACCAAAGCATAAATCCAATGCTTACGACCGAGCAGAAAACAGCTAA

Protein Analysis

278

Amino Acids

30.86

Weight (kDa)

5.51

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 19 - 125 5.8e-18 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AccB1I GGYRCC 2 cut(s) 180, 669
AccI GTMKAC 1 cut(s) 156
AciI CCGC 1 cut(s) 304
AcyI GRCGYC 2 cut(s) 489, 670
AfaI GTAC 6 cut(s) 13, 151, 274, 531, 627, 690
AfiI CCNNNNNNNGG 1 cut(s) 54
AgsI TTSAA 3 cut(s) 131, 520, 545
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 6 cut(s) 35, 221, 266, 609, 666, 834
AluI AGCT 6 cut(s) 35, 221, 266, 609, 666, 834
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 3 cut(s) 52, 569, 698
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 2 cut(s) 45, 538
ApaI GGGCCC 1 cut(s) 49
ApeKI GCWGC 2 cut(s) 263, 728
AspLEI GCGC 1 cut(s) 672
AspS9I GGNCC 3 cut(s) 45, 46, 301
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 301
BaeGI GKGCMC 3 cut(s) 49, 185, 406
BanI GGYRCC 2 cut(s) 180, 669
BanII GRGCYC 2 cut(s) 49, 557
BbsI GAAGAC 1 cut(s) 629
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 275, 715
BccI CCATC 2 cut(s) 685, 770
BciT130I CCWGG 1 cut(s) 450
BcoDI GTCTC 3 cut(s) 52, 569, 698
BfaI CTAG 2 cut(s) 582, 641
BfoI RGCGCY 1 cut(s) 673
BisI GCNGC 2 cut(s) 264, 729
BlpI GCTNAGC 2 cut(s) 217, 605
BlsI GCNGC 2 cut(s) 265, 730
Bme1390I CCNGG 1 cut(s) 450
Bme18I GGWCC 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 3 cut(s) 45, 46, 301
BmiI GGNNCC 5 cut(s) 47, 182, 370, 671, 771
BmrFI CCNGG 1 cut(s) 450
BmrI ACTGGG 3 cut(s) 373, 493, 745
BmuI ACTGGG 3 cut(s) 373, 493, 745
BpiI GAAGAC 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 53
Bpu1102I GCTNAGC 2 cut(s) 217, 605
BpuEI CTTGAG 1 cut(s) 722
BsaAI YACGTR 1 cut(s) 692
BsaHI GRCGYC 2 cut(s) 489, 670
BsaJI CCNNGG 1 cut(s) 449
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse1I ACTGG 4 cut(s) 368, 372, 488, 740
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 449
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 6 cut(s) 44, 60, 231, 389, 653, 741
BseNI ACTGG 4 cut(s) 368, 372, 488, 740
BseRI GAGGAG 2 cut(s) 70, 447
BseSI GKGCMC 3 cut(s) 49, 185, 406
BseXI GCAGC 2 cut(s) 275, 715
Bsh1285I CGRYCG 1 cut(s) 820
BshFI GGCC 2 cut(s) 47, 540
BshNI GGYRCC 2 cut(s) 180, 669
BsiEI CGRYCG 1 cut(s) 820
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 3 cut(s) 52, 569, 698
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 2 cut(s) 47, 540
BsoBI CYCGRG 1 cut(s) 429
Bsp120I GGGCCC 1 cut(s) 45
Bsp1286I GDGCHC 5 cut(s) 49, 185, 406, 439, 557
Bsp1407I TGTACA 1 cut(s) 11
Bsp1720I GCTNAGC 2 cut(s) 217, 605
BspACI CCGC 1 cut(s) 304
BspANI GGCC 2 cut(s) 47, 540
BspCNI CTCAG 6 cut(s) 45, 61, 230, 390, 654, 742
BspHI TCATGA 1 cut(s) 420
BspLI GGNNCC 5 cut(s) 47, 182, 370, 671, 771
BspT107I GGYRCC 2 cut(s) 180, 669
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 4 cut(s) 368, 372, 488, 740
BssECI CCNNGG 1 cut(s) 449
BssNI GRCGYC 2 cut(s) 489, 670
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 5 cut(s) 115, 127, 154, 277, 413
BstACI GRCGYC 2 cut(s) 489, 670
BstAUI TGTACA 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 4 cut(s) 121, 214, 261, 668
BstDEI CTNAG 7 cut(s) 53, 69, 217, 398, 605, 662, 750
BstF5I GGATG 1 cut(s) 648
BstH2I RGCGCY 1 cut(s) 673
BstHHI GCGC 1 cut(s) 672
BstMAI GTCTC 3 cut(s) 52, 569, 698
BstMCI CGRYCG 1 cut(s) 820
BstMWI GCNNNNNNNGC 2 cut(s) 218, 434
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 448
BstSLI GKGCMC 3 cut(s) 49, 185, 406
BstV1I GCAGC 2 cut(s) 275, 715
BstV2I GAAGAC 1 cut(s) 629
BsuRI GGCC 2 cut(s) 47, 540
BtsCI GGATG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 123, 379
Cac8I GCNNGC 4 cut(s) 121, 214, 261, 668
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 1 cut(s) 420
CfoI GCGC 1 cut(s) 672
Cfr13I GGNCC 3 cut(s) 45, 46, 301
Csp6I GTAC 6 cut(s) 12, 150, 273, 530, 626, 689
CviAII CATG 3 cut(s) 120, 355, 421
CviQI GTAC 6 cut(s) 12, 150, 273, 530, 626, 689
DdeI CTNAG 7 cut(s) 53, 69, 217, 398, 605, 662, 750
DinI GGCGCC 1 cut(s) 671
Eco24I GRGCYC 2 cut(s) 49, 557
Eco47I GGWCC 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 448
EcoT38I GRGCYC 2 cut(s) 49, 557
EgeI GGCGCC 1 cut(s) 671
EheI GGCGCC 1 cut(s) 671
FaeI CATG 3 cut(s) 123, 358, 424
FaqI GGGAC 1 cut(s) 500
FatI CATG 3 cut(s) 119, 354, 420
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 264, 729
FokI GGATG 1 cut(s) 635
FriOI GRGCYC 2 cut(s) 49, 557
Fsp4HI GCNGC 2 cut(s) 264, 729
FspBI CTAG 2 cut(s) 582, 641
GlaI GCGC 1 cut(s) 671
GluI GCNGC 2 cut(s) 264, 729
HaeII RGCGCY 1 cut(s) 673
HaeIII GGCC 2 cut(s) 47, 540
HhaI GCGC 1 cut(s) 672
Hin1I GRCGYC 2 cut(s) 489, 670
Hin1II CATG 3 cut(s) 123, 358, 424
Hin6I GCGC 1 cut(s) 670
HinP1I GCGC 1 cut(s) 670
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 33
HinfI GANTC 2 cut(s) 338, 722
Hpy166II GTNNAC 4 cut(s) 12, 157, 202, 280
Hpy188I TCNGA 6 cut(s) 40, 70, 90, 559, 721, 751
Hpy188III TCNNGA 4 cut(s) 107, 421, 641, 773
Hpy8I GTNNAC 4 cut(s) 12, 157, 202, 280
HpyAV CCTTC 2 cut(s) 514, 760
HpyCH4III ACNGT 5 cut(s) 115, 127, 154, 277, 413
HpyCH4IV ACGT 3 cut(s) 489, 532, 691
HpyCH4V TGCA 5 cut(s) 25, 123, 259, 377, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 434
HpyF3I CTNAG 7 cut(s) 53, 69, 217, 398, 605, 662, 750
HpySE526I ACGT 3 cut(s) 489, 532, 691
Hsp92I GRCGYC 2 cut(s) 489, 670
Hsp92II CATG 3 cut(s) 123, 358, 424
HspAI GCGC 1 cut(s) 670
KasI GGCGCC 1 cut(s) 669
LmnI GCTCC 4 cut(s) 368, 434, 472, 502
Lsp1109I GCAGC 2 cut(s) 275, 715
MaeI CTAG 2 cut(s) 582, 641
MaeII ACGT 3 cut(s) 489, 532, 691
MaeIII GTNAC 3 cut(s) 359, 479, 490
MboII GAAGA 1 cut(s) 629
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 5 cut(s) 49, 185, 406, 439, 557
MluCI AATT 2 cut(s) 378, 441
Mly113I GGCGCC 1 cut(s) 670
MlyI GAGTC 1 cut(s) 332
MnlI CCTC 4 cut(s) 48, 64, 313, 425
MseI TTAA 2 cut(s) 444, 510
MspA1I CMGCKG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 450
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 2 cut(s) 218, 434
NarI GGCGCC 1 cut(s) 670
NlaIII CATG 3 cut(s) 123, 358, 424
NlaIV GGNNCC 5 cut(s) 47, 182, 370, 671, 771
NmuCI GTSAC 1 cut(s) 490
NspI RCATGY 1 cut(s) 123
PaeI GCATGC 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 429
PagI TCATGA 1 cut(s) 420
PfeI GAWTC 1 cut(s) 722
PkrI GCNGC 2 cut(s) 265, 730
PleI GAGTC 1 cut(s) 332
PluTI GGCGCC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 5 cut(s) 47, 182, 370, 671, 771
PspOMI GGGCCC 1 cut(s) 45
PspPI GGNCC 3 cut(s) 45, 46, 301
PspXI VCTCGAGB 1 cut(s) 429
PstNI CAGNNNCTG 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 6 cut(s) 13, 151, 274, 531, 627, 690
RsaNI GTAC 6 cut(s) 12, 150, 273, 530, 626, 689
SaqAI TTAA 2 cut(s) 444, 510
SatI GCNGC 2 cut(s) 264, 729
Sau96I GGNCC 3 cut(s) 45, 46, 301
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 450
SduI GDGCHC 5 cut(s) 49, 185, 406, 439, 557
SfoI GGCGCC 1 cut(s) 671
Sfr274I CTCGAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 301
SlaI CTCGAG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 429, 701
SmoI CTYRAG 2 cut(s) 429, 701
SphI GCATGC 1 cut(s) 123
Sse9I AATT 2 cut(s) 378, 441
SsiI CCGC 1 cut(s) 304
SspDI GGCGCC 1 cut(s) 669
SspI AATATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 582, 641
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 5 cut(s) 115, 127, 154, 277, 413
TaiI ACGT 3 cut(s) 492, 535, 694
TaqI TCGA 1 cut(s) 430
TaqII GACCGA 1 cut(s) 834
TasI AATT 2 cut(s) 378, 441
TatI WGTACW 1 cut(s) 11
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 2 cut(s) 444, 510
Tru9I TTAA 2 cut(s) 444, 510
TscAI CASTG 2 cut(s) 130, 379
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 2 cut(s) 263, 728
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 5 cut(s) 54, 151, 260, 405, 409
TspRI CASTG 2 cut(s) 130, 379
VpaK11BI GGWCC 1 cut(s) 301
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 1 cut(s) 123
XhoI CTCGAG 1 cut(s) 429
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 2 cut(s) 582, 641
ZraI GACGTC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.