Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004194

Physical Location & Seq

Genomic Coordinates Reverse (-)

8351975 .. 8357602

Gene Structure 5628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1062000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGGACGAGTCTCCCGCCGAAACCGCCCCGGTTCCCGAGGCTAGGAACGGCGGCTCTGAGCGAACGCCGGAGGAGCTGGTGGACAAGTGCATCGCTCCCGTGAAGAAACAGTTCCTTCGCCCTCCCCCTATCAGAGGTCTCTCTAACGACCAGAACGACGCCGTCTCGGAGGGGAAATCAAAGGCTTCCCAGACTAGTGTGGTGAAGGAGAAGAAGTCCAAGCGGCAGCTCAAACGCGAACGCCAACAGGCAGTGAAATCGGCTGTAGGCATCTGCCCGGAGGTGGCGAAAAGAGGAGATGTTAGTCGGTGCCCGTACAAGGACAAATGCCGGTTCAGCCACGACGTAGAAGCTTATAAAGCTCAGAAACCTGACGACATCGAGGGAGAGTGTCCTTTCATGACTGCCCAAGAGGCATGCCCGTATGGATTGGCATGTAGGTTCTTGGGTTCGCATAAAGACGGTGTTGAAGGTGGAGATGTGAGTGCTCACAGGAGAAGCTCCGAGGTGAATGGATTGACCAAAGACGTTCAAAAGCTTTTGTGGAAGAATAAAATGAAGTTCCCCAAAGCAGATGCCAAACTCAAGGCTCTTGGGCTCGAGGGGAATGCCAAGTCAAAAATAAAGATATTAGAAGATAAGGAGGATGACCAGGTTGTTTCAAATAACTGTGATGTCACTGATGGAAATGGCTGTAGTGAATTGCCTGCTGAAATAGGAGAAGAGGGTGGCCTTGATGGAACATGTCCAGATGATGAATCTCGGCCTTCGAAGAAGACAAGATCTGTAATTGAAGAGGAGATTTGCTCTGGTGAAGTAGATGATGGAGGTGTAAGTGCTGCAAAAGAAGCTGCTGAGCAGACCTCTACAGCACCTGAACCAGAGGCTACTGTTGGTATTGTATTACCAGAGAGTGATAGAAGCCTAAGACTACACCCACGTGAAAGGAAGCTTATTGATTTTAGAGAAAAGTTGTACCTTGCACCTCTGACCACCGTTGGAAACCTTCCATTCCGAAGGGTTTGCAAAGTGTTAGGAGCAGATATAACATGTGGTGAAATGGCAATGTGCACAAATCTTTTGCAGGGTCAAGCATCAGAATGGGCACTGCTGAGGCGACATTCATCTGAGGATTTGTTTGGTGTTCAGATATGTGGAGCATATCCAGACACTGTGGCACACACTGTTGAGCTAATTGATCAGGAATGTCAAGTGGACTTTATTGATATAAATATGGGGTGCCCAATTGATATTGTTGTGAACAAGGGTGCTGGTTCAGCTCTTCTTACAAAACCCATGCGGATGAAAGGCATCGTACAAGCCGCTTCTGGTACTGTGGACTGTCCTATAACTATTAAGGTCAGGACAGCCTATTTTGAGGGGAAAAATCGCATTGATTCACTTATTGCGGACATGGGAAACTGGGGAGCTAGTGCAGTAACAATACATGGTCGATCTCGACAACAACGCTACAGCAAGCTTGCAGATTGGGAATATGTTTACCAGTGTGCCAGAAAGGCCCCTAAGGCTTTGCCAGTACTTGGAAATGGAGACATATTTTCATATGTAGATTGGAACAAGCACAAGGCTGAATGCCCTGAGCTGTCTACATGCATGATTGCTCGAGGAGCACTAATTAAGCCTTGGATTTTTACTGAAATAAAGGAGCAGAGGCACTGGGACATCAGTTCTGGGGAGCGATTAAATATATTGAAGGATTATGTACGTCTTGGCCTTGAACATTGGGGCTCTGACACAAAAGGTGTGGAGACAACTAGGCGTTTTCTGTTAGAATGGCTTAGCTACACTTGTAGATACGTACCTGTGGGTCTTCTAGATGTCATTCCACAACGGTTGAACTGGCGCCCGCCATCCTATTATGGCCGTGATGACCTTGAGACACTAATGGCCTCTGATTCTGCGGCTGATTGGGTTAGGATCTCTGAGATATTGCTTGGCAAGGTTCCAGATGGCTTTACATTTGCACCAAAGCACAAATCCAATTCTTACGACCGAGCTGAAAACGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002943 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016491 GO:0016627 GO:0017150 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0055114 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dus zf-CCCH

Protein Analysis

677

Amino Acids

74.81

Weight (kDa)

6.12

Isoelectric Point (pI)

42.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCCH_DUS3L	PF25585	90 - 122	1.3e-10	DUS3L CCCH zinc finger domain
zf-CCCH_DUS3L	PF25585	123 - 154	1.3e-06	DUS3L CCCH zinc finger domain
Dus	PF01207	327 - 562	8.3e-49	Dihydrouridine synthase (Dus)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38890
fragaria_vesca	FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690
malus_domestica	MD05G1054400.v1.1 MD10G1062000.v1.1
prunus_persica	Prupe.8G082800_v2.0.a1
pyrus_communis	pycom10g04770
rosa_chinensis	RchiOBHm_Chr6g0268371 RchiOBHm_Chr6g0268451 RchiOBHm_Chr6g0268461 RchiOBHm_Chr6g0271651 RchiOBHm_Chr6g0271661
rosa_laevigata	RLG00000013910
rosa_multiflora	Rmu_sc0002404.1_g000009 Rmu_sc0002404.1_g000017 Rmu_sc0007949.1_g000004 Rmu_sc0008184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00199830
rosa_rugosa	Rorug06G0041000 Rorug06G0042100
rosa_samantha	Rh6AG161000 Rh6BG165400 Rh6CG152700 Rh6CG159400 Rh6DG151200
rosa_wichuraiana	Rw6G013920 Rw6G013960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	357
AccB1I	GGYRCC	3 cut(s)	309, 1239, 1863
AccB7I	CCANNNNNTGG	1 cut(s)	1541
AccI	GTMKAC	1 cut(s)	1607
AccII	CGCG	1 cut(s)	237
AciI	CCGC	9 cut(s)	15, 24, 51, 223, 1300, 1323, 1409, 1868, 1922
AclWI	GGATC	1 cut(s)	1946
AcoI	YGGCCR	1 cut(s)	1882
AcvI	CACGTG	1 cut(s)	941
AcyI	GRCGYC	2 cut(s)	159, 1864
AfaI	GTAC	7 cut(s)	317, 977, 1317, 1333, 1539, 1725, 1821
AfiI	CCNNNNNNNGG	5 cut(s)	42, 134, 283, 319, 1541
AflIII	ACRYGT	2 cut(s)	743, 1049
AgsI	TTSAA	7 cut(s)	470, 533, 663, 794, 1714, 1739, 1858
AhlI	ACTAGT	1 cut(s)	194
AjnI	CCWGG	1 cut(s)	651
AleI	CACNNNNGTG	1 cut(s)	939
Alw21I	GWGCWC	3 cut(s)	490, 1073, 1633
Alw26I	GTCTC	6 cut(s)	15, 143, 169, 1545, 1763, 1892
Alw44I	GTGCAC	1 cut(s)	1069
AlwI	GGATC	1 cut(s)	1946
AlwNI	CAGNNNCTG	2 cut(s)	875, 1172
Ama87I	CYCGRG	3 cut(s)	35, 599, 1623
AoxI	GGCC	6 cut(s)	730, 764, 1518, 1732, 1882, 1908
ApaLI	GTGCAC	1 cut(s)	1069
ApeKI	GCWGC	3 cut(s)	226, 839, 851
Asp700I	GAANNNNTTC	1 cut(s)	110
AspLEI	GCGC	1 cut(s)	1866
AspS9I	GGNCC	1 cut(s)	1519
AsuC2I	CCSGG	2 cut(s)	29, 278
AsuHPI	GGTGA	4 cut(s)	214, 520, 824, 1067
AsuII	TTCGAA	1 cut(s)	770
AvaI	CYCGRG	3 cut(s)	35, 599, 1623
AxyI	CCTNAGG	1 cut(s)	1524
BaeGI	GKGCMC	4 cut(s)	314, 1073, 1108, 1244
BanI	GGYRCC	3 cut(s)	309, 1239, 1863
BanII	GRGCYC	2 cut(s)	600, 1751
BbrPI	CACGTG	1 cut(s)	941
BbsI	GAAGAC	2 cut(s)	782, 1823
Bbv12I	GWGCWC	3 cut(s)	490, 1073, 1633
BbvCI	CCTCAGC	1 cut(s)	1112
BbvI	GCAGC	3 cut(s)	238, 826, 838
BccI	CCATC	5 cut(s)	677, 731, 818, 1879, 1964
BceAI	ACGGC	3 cut(s)	64, 146, 1869
BcgI	CGANNNNNNTGC	6 cut(s)	240, 274, 590, 624, 1005, 1039
BciT130I	CCWGG	1 cut(s)	653
BclI	TGATCA	1 cut(s)	1198
BcnI	CCSGG	2 cut(s)	29, 278
BcoDI	GTCTC	6 cut(s)	15, 143, 169, 1545, 1763, 1892
BcuI	ACTAGT	1 cut(s)	194
BfaI	CTAG	5 cut(s)	42, 195, 1431, 1776, 1835
BfmI	CTRYAG	4 cut(s)	264, 694, 867, 1471
BfoI	RGCGCY	1 cut(s)	1867
BglI	GCCNNNNNGGC	3 cut(s)	413, 1517, 1526
BglII	AGATCT	1 cut(s)	782
BisI	GCNGC	7 cut(s)	52, 224, 227, 840, 852, 1323, 1923
BlpI	GCTNAGC	2 cut(s)	855, 1799
BlsI	GCNGC	7 cut(s)	53, 225, 228, 841, 853, 1324, 1924
BmcAI	AGTACT	1 cut(s)	1539
Bme1390I	CCNGG	3 cut(s)	29, 278, 653
BmeT110I	CYCGRG	3 cut(s)	35, 599, 1623
BmgT120I	GGNCC	1 cut(s)	1519
BmiI	GGNNCC	6 cut(s)	33, 311, 1241, 1521, 1865, 1965
BmrFI	CCNGG	3 cut(s)	29, 278, 653
BmrI	ACTGGG	2 cut(s)	1432, 1687
BmsI	GCATC	5 cut(s)	99, 279, 565, 1103, 1320
BmuI	ACTGGG	2 cut(s)	1432, 1687
BpiI	GAAGAC	2 cut(s)	782, 1823
BplI	GAGNNNNNCTC	4 cut(s)	791, 823, 848, 880
Bpu10I	CCTNAGC	2 cut(s)	1112, 1599
Bpu1102I	GCTNAGC	2 cut(s)	855, 1799
Bpu14I	TTCGAA	1 cut(s)	770
BpuEI	CTTGAG	2 cut(s)	569, 1916
BpuMI	CCSGG	2 cut(s)	29, 278
BsaAI	YACGTR	2 cut(s)	941, 1819
BsaHI	GRCGYC	2 cut(s)	159, 1864
BsaI	GGTCTC	1 cut(s)	143
BsaJI	CCNNGG	4 cut(s)	27, 36, 504, 1643
BsaXI	ACNNNNNCTCC	2 cut(s)	200, 230
Bsc4I	CCNNNNNNNGG	5 cut(s)	42, 134, 283, 319, 1541
Bse118I	RCCGGY	1 cut(s)	330
Bse1I	ACTGG	5 cut(s)	1427, 1504, 1535, 1682, 1865
Bse21I	CCTNAGG	1 cut(s)	1524
Bse3DI	GCAATG	1 cut(s)	1071
BseBI	CCWGG	1 cut(s)	653
BseDI	CCNNGG	4 cut(s)	27, 36, 504, 1643
BseGI	GGATG	3 cut(s)	652, 1308, 1871
BseLI	CCNNNNNNNGG	5 cut(s)	42, 134, 283, 319, 1541
BseMI	GCAATG	1 cut(s)	1071
BseMII	CTCAG	7 cut(s)	48, 377, 846, 1103, 1119, 1590, 1935
BseNI	ACTGG	5 cut(s)	1427, 1504, 1535, 1682, 1865
BseRI	GAGGAG	4 cut(s)	86, 309, 812, 1641
BseSI	GKGCMC	4 cut(s)	314, 1073, 1108, 1244
BseXI	GCAGC	3 cut(s)	238, 826, 838
BsgI	GTGCAG	1 cut(s)	1455
Bsh1236I	CGCG	1 cut(s)	237
Bsh1285I	CGRYCG	1 cut(s)	2014
BshFI	GGCC	6 cut(s)	732, 766, 1520, 1734, 1884, 1910
BshNI	GGYRCC	3 cut(s)	309, 1239, 1863
BsiEI	CGRYCG	1 cut(s)	2014
BsiHKAI	GWGCWC	3 cut(s)	490, 1073, 1633
BsiHKCI	CYCGRG	3 cut(s)	35, 599, 1623
BsiSI	CCGG	4 cut(s)	29, 68, 278, 331
BslFI	GGGAC	1 cut(s)	1694
BslI	CCNNNNNNNGG	5 cut(s)	42, 134, 283, 319, 1541
BsmAI	GTCTC	6 cut(s)	15, 143, 169, 1545, 1763, 1892
BsmBI	CGTCTC	1 cut(s)	169
BsmFI	GGGAC	1 cut(s)	1694
BsmI	GAATGC	2 cut(s)	613, 1598
BsnI	GGCC	6 cut(s)	732, 766, 1520, 1734, 1884, 1910
Bso31I	GGTCTC	1 cut(s)	143
BsoBI	CYCGRG	3 cut(s)	35, 599, 1623
Bsp119I	TTCGAA	1 cut(s)	770
Bsp1286I	GDGCHC	8 cut(s)	314, 490, 600, 1073, 1108, 1244, 1633, 1751
Bsp143I	GATC	4 cut(s)	782, 1198, 1454, 1938
Bsp1720I	GCTNAGC	2 cut(s)	855, 1799
BspACI	CCGC	9 cut(s)	15, 24, 51, 223, 1300, 1323, 1409, 1868, 1922
BspANI	GGCC	6 cut(s)	732, 766, 1520, 1734, 1884, 1910
BspCNI	CTCAG	7 cut(s)	49, 376, 847, 1104, 1120, 1591, 1936
BspFNI	CGCG	1 cut(s)	237
BspHI	TCATGA	1 cut(s)	399
BspLI	GGNNCC	6 cut(s)	33, 311, 1241, 1521, 1865, 1965
BspPI	GGATC	1 cut(s)	1946
BspQI	GCTCTTC	1 cut(s)	1287
BspT104I	TTCGAA	1 cut(s)	770
BspT107I	GGYRCC	3 cut(s)	309, 1239, 1863
BspTNI	GGTCTC	1 cut(s)	143
BsrDI	GCAATG	1 cut(s)	1071
BsrFI	RCCGGY	1 cut(s)	330
BsrI	ACTGG	5 cut(s)	1427, 1504, 1535, 1682, 1865
BssAI	RCCGGY	1 cut(s)	330
BssECI	CCNNGG	4 cut(s)	27, 36, 504, 1643
BssMI	GATC	4 cut(s)	782, 1198, 1454, 1938
BssNI	GRCGYC	2 cut(s)	159, 1864
BssT1I	CCWWGG	1 cut(s)	1643
Bst2UI	CCWGG	1 cut(s)	653
Bst6I	CTCTTC	3 cut(s)	717, 789, 1287
BstACI	GRCGYC	2 cut(s)	159, 1864
BstBAI	YACGTR	2 cut(s)	941, 1819
BstBI	TTCGAA	1 cut(s)	770
BstC8I	GCNNGC	5 cut(s)	418, 708, 1478, 1482, 1868
BstENI	CCTNNNNNAGG	1 cut(s)	132
BstF5I	GGATG	3 cut(s)	652, 1308, 1871
BstFNI	CGCG	1 cut(s)	237
BstH2I	RGCGCY	1 cut(s)	1867
BstHHI	GCGC	1 cut(s)	1866
BstKTI	GATC	4 cut(s)	785, 1201, 1457, 1941
BstMAI	GTCTC	6 cut(s)	15, 143, 169, 1545, 1763, 1892
BstMBI	GATC	4 cut(s)	782, 1198, 1454, 1938
BstMCI	CGRYCG	1 cut(s)	2014
BstNI	CCWGG	1 cut(s)	653
BstNSI	RCATGY	5 cut(s)	420, 438, 747, 1053, 1614
BstSCI	CCNGG	3 cut(s)	27, 276, 651
BstSFI	CTRYAG	4 cut(s)	264, 694, 867, 1471
BstSLI	GKGCMC	4 cut(s)	314, 1073, 1108, 1244
BstSNI	TACGTA	1 cut(s)	1819
BstUI	CGCG	1 cut(s)	237
BstV1I	GCAGC	3 cut(s)	238, 826, 838
BstV2I	GAAGAC	2 cut(s)	782, 1823
BstX2I	RGATCY	2 cut(s)	782, 1938
BstYI	RGATCY	2 cut(s)	782, 1938
Bsu36I	CCTNAGG	1 cut(s)	1524
BsuRI	GGCC	6 cut(s)	732, 766, 1520, 1734, 1884, 1910
BtgZI	GCGATG	1 cut(s)	76
BtsCI	GGATG	3 cut(s)	652, 1308, 1871
BtsI	GCAGTG	2 cut(s)	258, 1106
BtsIMutI	CAGTG	7 cut(s)	258, 678, 1106, 1170, 1182, 1511, 1675
Cac8I	GCNNGC	5 cut(s)	418, 708, 1478, 1482, 1868
CaiI	CAGNNNCTG	2 cut(s)	875, 1172
CciI	TCATGA	1 cut(s)	399
CfoI	GCGC	1 cut(s)	1866
Cfr10I	RCCGGY	1 cut(s)	330
Cfr13I	GGNCC	1 cut(s)	1519
CseI	GACGC	1 cut(s)	167
CsiI	ACCWGGT	1 cut(s)	651
Csp6I	GTAC	7 cut(s)	316, 976, 1316, 1332, 1538, 1724, 1820
CspCI	CAANNNNNGTGG	4 cut(s)	1746, 1781, 1836, 1871
CviQI	GTAC	7 cut(s)	316, 976, 1316, 1332, 1538, 1724, 1820
DinI	GGCGCC	1 cut(s)	1865
DpnI	GATC	4 cut(s)	784, 1200, 1456, 1940
DpnII	GATC	4 cut(s)	782, 1198, 1454, 1938
EaeI	YGGCCR	1 cut(s)	1882
Eam1104I	CTCTTC	3 cut(s)	717, 789, 1287
EarI	CTCTTC	3 cut(s)	717, 789, 1287
Eco105I	TACGTA	1 cut(s)	1819
Eco130I	CCWWGG	1 cut(s)	1643
Eco24I	GRGCYC	2 cut(s)	600, 1751
Eco31I	GGTCTC	1 cut(s)	143
Eco72I	CACGTG	1 cut(s)	941
Eco81I	CCTNAGG	1 cut(s)	1524
Eco88I	CYCGRG	3 cut(s)	35, 599, 1623
EcoNI	CCTNNNNNAGG	1 cut(s)	132
EcoO109I	RGGNCCY	1 cut(s)	1519
EcoRII	CCWGG	1 cut(s)	651
EcoT14I	CCWWGG	1 cut(s)	1643
EcoT22I	ATGCAT	1 cut(s)	1616
EcoT38I	GRGCYC	2 cut(s)	600, 1751
EgeI	GGCGCC	1 cut(s)	1865
EheI	GGCGCC	1 cut(s)	1865
ErhI	CCWWGG	1 cut(s)	1643
Esp3I	CGTCTC	1 cut(s)	169
FaqI	GGGAC	1 cut(s)	1694
FauI	CCCGC	2 cut(s)	22, 1875
FauNDI	CATATG	1 cut(s)	1564
FbaI	TGATCA	1 cut(s)	1198
FblI	GTMKAC	1 cut(s)	1607
Fnu4HI	GCNGC	7 cut(s)	52, 224, 227, 840, 852, 1323, 1923
FokI	GGATG	3 cut(s)	659, 1315, 1858
FriOI	GRGCYC	2 cut(s)	600, 1751
Fsp4HI	GCNGC	7 cut(s)	52, 224, 227, 840, 852, 1323, 1923
FspBI	CTAG	5 cut(s)	42, 195, 1431, 1776, 1835
GlaI	GCGC	1 cut(s)	1865
GluI	GCNGC	7 cut(s)	52, 224, 227, 840, 852, 1323, 1923
HaeII	RGCGCY	1 cut(s)	1867
HaeIII	GGCC	6 cut(s)	732, 766, 1520, 1734, 1884, 1910
HapII	CCGG	4 cut(s)	29, 68, 278, 331
HgaI	GACGC	1 cut(s)	167
HhaI	GCGC	1 cut(s)	1866
Hin1I	GRCGYC	2 cut(s)	159, 1864
Hin6I	GCGC	1 cut(s)	1864
HinP1I	GCGC	1 cut(s)	1864
HindIII	AAGCTT	4 cut(s)	351, 536, 950, 1478
HinfI	GANTC	4 cut(s)	8, 758, 1397, 1916
HpaII	CCGG	4 cut(s)	29, 68, 278, 331
HphI	GGTGA	4 cut(s)	214, 520, 824, 1067
Hpy166II	GTNNAC	7 cut(s)	82, 1071, 1216, 1261, 1339, 1501, 1608
Hpy188III	TCNNGA	9 cut(s)	35, 400, 749, 1166, 1202, 1363, 1458, 1835, 1967
Hpy8I	GTNNAC	7 cut(s)	82, 1071, 1216, 1261, 1339, 1501, 1608
Hpy99I	CGWCG	2 cut(s)	161, 347
HpyAV	CCTTC	7 cut(s)	125, 199, 464, 777, 1011, 1016, 1708
HpyCH4IV	ACGT	5 cut(s)	345, 528, 940, 1726, 1818
HpySE526I	ACGT	5 cut(s)	345, 528, 940, 1726, 1818
Hsp92I	GRCGYC	2 cut(s)	159, 1864
HspAI	GCGC	1 cut(s)	1864
KasI	GGCGCC	1 cut(s)	1863
Ksp22I	TGATCA	1 cut(s)	1198
Kzo9I	GATC	4 cut(s)	782, 1198, 1454, 1938
LguI	GCTCTTC	1 cut(s)	1287
LmnI	GCTCC	9 cut(s)	73, 100, 506, 1037, 1157, 1427, 1628, 1666, 1696
Lsp1109I	GCAGC	3 cut(s)	238, 826, 838
LweI	GCATC	5 cut(s)	99, 279, 565, 1103, 1320
MabI	ACCWGGT	1 cut(s)	651
MaeI	CTAG	5 cut(s)	42, 195, 1431, 1776, 1835
MaeII	ACGT	5 cut(s)	345, 528, 940, 1726, 1818
MaeIII	GTNAC	2 cut(s)	676, 1438
MalI	GATC	4 cut(s)	784, 1200, 1456, 1940
MboI	GATC	4 cut(s)	782, 1198, 1454, 1938
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	2 cut(s)	782, 1938
MhlI	GDGCHC	8 cut(s)	314, 490, 600, 1073, 1108, 1244, 1633, 1751
MluCI	AATT	6 cut(s)	701, 789, 1194, 1245, 1635, 2002
Mly113I	GGCGCC	1 cut(s)	1864
MlyI	GAGTC	1 cut(s)	17
MmeI	TCCRAC	1 cut(s)	979
Mph1103I	ATGCAT	1 cut(s)	1616
MroXI	GAANNNNTTC	1 cut(s)	110
MseI	TTAA	3 cut(s)	1356, 1638, 1703
MslI	CAYNNNNRTG	3 cut(s)	939, 1099, 1301
MspI	CCGG	4 cut(s)	29, 68, 278, 331
MspR9I	CCNGG	3 cut(s)	29, 278, 653
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	2 cut(s)	613, 1598
MvaI	CCWGG	1 cut(s)	653
MvnI	CGCG	1 cut(s)	237
NarI	GGCGCC	1 cut(s)	1864
NciI	CCSGG	2 cut(s)	29, 278
NdeI	CATATG	1 cut(s)	1564
NdeII	GATC	4 cut(s)	782, 1198, 1454, 1938
NlaIV	GGNNCC	6 cut(s)	33, 311, 1241, 1521, 1865, 1965
NmeAIII	GCCGAG	1 cut(s)	742
NmuCI	GTSAC	1 cut(s)	676
NsiI	ATGCAT	1 cut(s)	1616
NspI	RCATGY	5 cut(s)	420, 438, 747, 1053, 1614
NspV	TTCGAA	1 cut(s)	770
OliI	CACNNNNGTG	1 cut(s)	939
PaeI	GCATGC	1 cut(s)	420
PaeR7I	CTCGAG	2 cut(s)	599, 1623
PagI	TCATGA	1 cut(s)	399
PciI	ACATGT	2 cut(s)	743, 1049
PciSI	GCTCTTC	1 cut(s)	1287
PcsI	WCGNNNNNNNCGW	1 cut(s)	153
PctI	GAATGC	2 cut(s)	613, 1598
PdmI	GAANNNNTTC	1 cut(s)	110
PfeI	GAWTC	3 cut(s)	758, 1397, 1916
PflFI	GACNNNGTC	1 cut(s)	161
PflMI	CCANNNNNTGG	1 cut(s)	1541
PkrI	GCNGC	7 cut(s)	53, 225, 228, 841, 853, 1324, 1924
PleI	GAGTC	1 cut(s)	16
PluTI	GGCGCC	1 cut(s)	1867
PmaCI	CACGTG	1 cut(s)	941
PmlI	CACGTG	1 cut(s)	941
PpsI	GAGTC	1 cut(s)	16
Ppu21I	YACGTR	2 cut(s)	941, 1819
PscI	ACATGT	2 cut(s)	743, 1049
PsiI	TTATAA	1 cut(s)	357
Psp6I	CCWGG	1 cut(s)	651
PspCI	CACGTG	1 cut(s)	941
PspGI	CCWGG	1 cut(s)	651
PspN4I	GGNNCC	6 cut(s)	33, 311, 1241, 1521, 1865, 1965
PspPI	GGNCC	1 cut(s)	1519
PspXI	VCTCGAGB	2 cut(s)	599, 1623
PstNI	CAGNNNCTG	2 cut(s)	875, 1172
PsuI	RGATCY	2 cut(s)	782, 1938
PsyI	GACNNNGTC	1 cut(s)	161
RsaI	GTAC	7 cut(s)	317, 977, 1317, 1333, 1539, 1725, 1821
RsaNI	GTAC	7 cut(s)	316, 976, 1316, 1332, 1538, 1724, 1820
RseI	CAYNNNNRTG	3 cut(s)	939, 1099, 1301
SapI	GCTCTTC	1 cut(s)	1287
SaqAI	TTAA	3 cut(s)	1356, 1638, 1703
SatI	GCNGC	7 cut(s)	52, 224, 227, 840, 852, 1323, 1923
Sau3AI	GATC	4 cut(s)	782, 1198, 1454, 1938
Sau96I	GGNCC	1 cut(s)	1519
ScaI	AGTACT	1 cut(s)	1539
SchI	GAGTC	1 cut(s)	17
ScrFI	CCNGG	3 cut(s)	29, 278, 653
SduI	GDGCHC	8 cut(s)	314, 490, 600, 1073, 1108, 1244, 1633, 1751
SexAI	ACCWGGT	1 cut(s)	651
SfaNI	GCATC	5 cut(s)	99, 279, 565, 1103, 1320
SfcI	CTRYAG	4 cut(s)	264, 694, 867, 1471
SfoI	GGCGCC	1 cut(s)	1865
Sfr274I	CTCGAG	2 cut(s)	599, 1623
SfuI	TTCGAA	1 cut(s)	770
SlaI	CTCGAG	2 cut(s)	599, 1623
SmiMI	CAYNNNNRTG	3 cut(s)	939, 1099, 1301
SmlI	CTYRAG	4 cut(s)	584, 599, 1623, 1895
SmoI	CTYRAG	4 cut(s)	584, 599, 1623, 1895
SnaBI	TACGTA	1 cut(s)	1819
SpeI	ACTAGT	1 cut(s)	194
SphI	GCATGC	1 cut(s)	420
Sse9I	AATT	6 cut(s)	701, 789, 1194, 1245, 1635, 2002
SsiI	CCGC	9 cut(s)	15, 24, 51, 223, 1300, 1323, 1409, 1868, 1922
SspDI	GGCGCC	1 cut(s)	1863
SspMI	CTAG	5 cut(s)	42, 195, 1431, 1776, 1835
StyD4I	CCNGG	3 cut(s)	27, 276, 651
StyI	CCWWGG	1 cut(s)	1643
TaiI	ACGT	5 cut(s)	348, 531, 943, 1729, 1821
TaqI	TCGA	6 cut(s)	381, 600, 770, 1453, 1459, 1624
TaqII	GACCGA	1 cut(s)	2028
TasI	AATT	6 cut(s)	701, 789, 1194, 1245, 1635, 2002
TatI	WGTACW	1 cut(s)	1537
TauI	GCSGC	4 cut(s)	54, 226, 1325, 1925
TfiI	GAWTC	3 cut(s)	758, 1397, 1916
Tru1I	TTAA	3 cut(s)	1356, 1638, 1703
Tru9I	TTAA	3 cut(s)	1356, 1638, 1703
TscAI	CASTG	7 cut(s)	258, 685, 1113, 1177, 1189, 1511, 1682
TseFI	GTSAC	1 cut(s)	676
TseI	GCWGC	3 cut(s)	226, 839, 851
Tsp45I	GTSAC	1 cut(s)	676
TspDTI	ATGAA	6 cut(s)	388, 572, 771, 1113, 1319, 1551
TspRI	CASTG	7 cut(s)	258, 685, 1113, 1177, 1189, 1511, 1682
Tth111I	GACNNNGTC	1 cut(s)	161
Van91I	CCANNNNNTGG	1 cut(s)	1541
VneI	GTGCAC	1 cut(s)	1069
XagI	CCTNNNNNAGG	1 cut(s)	132
XbaI	TCTAGA	1 cut(s)	1834
XceI	RCATGY	5 cut(s)	420, 438, 747, 1053, 1614
XhoI	CTCGAG	2 cut(s)	599, 1623
XmiI	GTMKAC	1 cut(s)	1607
XmnI	GAANNNNTTC	1 cut(s)	110
XspI	CTAG	5 cut(s)	42, 195, 1431, 1776, 1835
ZrmI	AGTACT	1 cut(s)	1539
Zsp2I	ATGCAT	1 cut(s)	1616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.