Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0004194

Physical Location & Seq

Genomic Coordinates Forward (+)

21837721 .. 21844093

Gene Structure 6373 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG151200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGGCGACGACACTCCTGCCGCAACTGGAACGGGCCCAGAGGTCTCCAACGGCGTGGCGGCGGCGGCGGCTGAGAGGACGCCGGAGGAGCTCGTAGCCAAGTCCATCGCTCCGGTGAAGAAACAGTACCTTCGCCCTCCTCCCATCAGACCTTGCTCTAAGGATCAAAACGACGCCGTCTGTGAGGGCAACGCGAAGCCTGCACAGTCGAACGTAGTGAAGGAGAAGAAGTCCAAGCGGCAGCTTAAGCGTGAACGCCGCGAGGTACAGAAATCGCCCCTCGGTATCTGTCCAGAGGTAGCAAAGAAAGGAGATGTTAGTCGGTGTCCATATAATGATAAGTGCCGCTACAGCCACGACATAGAAGCCTATAAGGCTCAGAAACCTGAGGATATAGAGGGAGAATGTCCTTTTATGGCTATCGATGAGCCATGCCCTTATGGTTTGTCATGTAGGTTCTTGGGTACACATAAAAATGGTGTTGCAGATGGAGATGTGAATGCTCGTAGAAGAAGCTCTGAGATGAATGGATTAAGCAAAAATGTTCAAAAGCTTTTGTGGAAAAATAAAATGAAGTTCACCAAGGCTGATGCCAAACTCAAGGCTCTTGGTCTCATGGGGCATGCCAATTCAAAAATAATTGCCATGTCAAAGGAGAATGGGTCGGTGGTTTCAAAGAATTGTGATGTTACTGATGGAAATGGCTGTAGTGAATTTCCCAGTAACACAGATGATAAAGTGGAGTTGACTGCTGAAATAAAAGAAAAAAGACGTGTTGATGAAAAGTTTCCATCTGATGAAATGGAGTTGTCTGCTGAAGTGAAAGAAGAGGGAGGTGCTAATGAAACATTTCCATCTGACGATTCTAGGCCTTCAAAGAAGTCAAGATCTGTAATTGAAGAGGAGTTTGGTTCTGGTGAAGCAGATTATGGAGGCGTGAGAGCTGCAGGAAAAGTTGTTGGGCAAACCTGCACGGAACCTGAACCAGAAGCTGCAGCTAACATTGAATTCTCAGAGAATGATAGAAGTTTAAAGACACACCCACGTGAAAAGAAGCTTATTGACTTCAGTGAAAAGTTGTACCTTGCACCTCTGACCACAGTCGGAAATCTTCCATTTCGAAGGGTTTGCAAAGGTTTAGGGGCAGATATAACATGTGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCGTCGGAATGGGCCTTGCTGAGGAGACATTCATCTGAGGATTTGTTTGGCGTTCAGATATGTGGGGCATATCCAGACACCGTAGCACGCACTGTTGAACTTATAGAACAGGAATGTACCGTAGACTTCATTGATATAAATATGGGGTGCCCAATAGATATTGTTGTGAACAAGGGTGCTGGCTCAGCTCTTCTAACAAAACCAATGCGAATGAAAGGCATTGTGCAAGCAGCTTCTGGTACAGTTGACATTCCTATGACTATCAAGGTCCGCACAGCCTATTTTGAGGGGAAAAATCGCATTGACTCTCTTATTGCTGACATGGGCAACTGGGGAGCCAGTGCAGTAACAATACATGGTCGATCACGACAGCAACGCTACAGCAAGCTTGCAGATTGGGAATATGTGTACCAGTGTGCTAAAAAAGCCCCTGATTCTTTGCAAGTCCTTGGAAATGGAGATGTATTTTCGTATGTAGATTGGAACAAGCACAAGGCTGAGTGCCCTGAACTGTCTTCATGCATGATTGCTCGAGGAGCACTAATTAAGCCTTGGATTTTTACAGAAATAAAGGAGCAGAGGCACTGGGACGTCACTTCTGGGGAGCGATTTAATATTTTGAAGGATTATGTACGTTGTGGCCTTGAACATTGGGGCTCTGACACAAAAGGTGTCGAGACAACTAGGCGTTTTCTGTTAGAATGGCTTAGCTACACTTGTAGATATGTACCTGTTGGTCTTCTAGATGTCATCCCACAACGACTGAGCTGGCGCCCACCATCTTATTTTGGACGTGATGACCTTGAGACACTAATGGCTTCTGATTCTGCCGCTGACTGGGTTAGGCTCTCTGAGATGTTGCTTGGGAAGGTTCCAGATGGTTTCACATTTGCACCAAAGCATAAATCCAATGCTTACGACCGAGCAGAAAACGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002943 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016491 GO:0016627 GO:0017150 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0055114 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dus zf-CCCH

Protein Analysis

711

Amino Acids

78.71

Weight (kDa)

6.61

Isoelectric Point (pI)

44.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCCH_DUS3L	PF25585	94 - 127	1.6e-10	DUS3L CCCH zinc finger domain
zf-CCCH_DUS3L	PF25585	128 - 159	2.7e-07	DUS3L CCCH zinc finger domain
Dus	PF01207	362 - 621	2e-48	Dihydrouridine synthase (Dus)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38890
fragaria_vesca	FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690
malus_domestica	MD05G1054400.v1.1 MD10G1062000.v1.1
prunus_persica	Prupe.8G082800_v2.0.a1
pyrus_communis	pycom10g04770
rosa_chinensis	RchiOBHm_Chr6g0268371 RchiOBHm_Chr6g0268451 RchiOBHm_Chr6g0268461 RchiOBHm_Chr6g0271651 RchiOBHm_Chr6g0271661
rosa_laevigata	RLG00000013910
rosa_multiflora	Rmu_sc0002404.1_g000009 Rmu_sc0002404.1_g000017 Rmu_sc0007949.1_g000004 Rmu_sc0008184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00199830
rosa_rugosa	Rorug06G0041000 Rorug06G0042100
rosa_samantha	Rh6AG161000 Rh6BG165400 Rh6CG152700 Rh6CG159400 Rh6DG151200
rosa_wichuraiana	Rw6G013920 Rw6G013960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1791
Acc36I	ACCTGC	1 cut(s)	977
AccB1I	GGYRCC	2 cut(s)	1344, 1968
AccI	GTMKAC	1 cut(s)	1320
AccII	CGCG	2 cut(s)	194, 261
AclWI	GGATC	1 cut(s)	171
AcsI	RAATTY	2 cut(s)	713, 1007
AcuI	CTGAAG	2 cut(s)	837, 1051
AcvI	CACGTG	1 cut(s)	1046
AcyI	GRCGYC	4 cut(s)	80, 174, 1788, 1969
AfiI	CCNNNNNNNGG	2 cut(s)	1218, 2034
AflII	CTTAAG	1 cut(s)	245
AflIII	ACRYGT	2 cut(s)	772, 1154
AgsI	TTSAA	9 cut(s)	548, 633, 675, 876, 899, 1007, 1295, 1819, 1844
AjiI	CACGTC	2 cut(s)	773, 1991
AjuI	GAANNNNNNNTTGG	2 cut(s)	891, 923
AleI	CACNNNNGTG	1 cut(s)	1044
Alw21I	GWGCWC	2 cut(s)	93, 1738
Alw26I	GTCTC	5 cut(s)	49, 617, 1216, 1868, 1997
AlwI	GGATC	1 cut(s)	171
AlwNI	CAGNNNCTG	2 cut(s)	992, 1433
Ama87I	CYCGRG	1 cut(s)	1728
AoxI	GGCC	4 cut(s)	34, 869, 1209, 1837
ApaI	GGGCCC	1 cut(s)	38
ApeKI	GCWGC	5 cut(s)	241, 944, 992, 995, 1427
ApoI	RAATTY	2 cut(s)	713, 1007
Asp700I	GAANNNNTTC	2 cut(s)	786, 849
AspLEI	GCGC	1 cut(s)	1971
AspS9I	GGNCC	4 cut(s)	34, 35, 1209, 1465
AsuHPI	GGTGA	4 cut(s)	127, 571, 929, 1172
AsuII	TTCGAA	1 cut(s)	1120
AvaI	CYCGRG	1 cut(s)	1728
AvaII	GGWCC	1 cut(s)	1465
AxyI	CCTNAGG	1 cut(s)	387
BaeGI	GKGCMC	3 cut(s)	38, 1349, 1703
BanI	GGYRCC	2 cut(s)	1344, 1968
BanII	GRGCYC	3 cut(s)	38, 93, 1856
BarI	GAAGNNNNNNTAC	2 cut(s)	110, 142
BbrPI	CACGTG	1 cut(s)	1046
BbsI	GAAGAC	2 cut(s)	1704, 1928
Bbv12I	GWGCWC	2 cut(s)	93, 1738
BbvCI	CCTCAGC	1 cut(s)	1217
BbvI	GCAGC	5 cut(s)	253, 931, 979, 1007, 1439
BccI	CCATC	8 cut(s)	113, 152, 482, 689, 799, 862, 1984, 2069
BceAI	ACGGC	2 cut(s)	67, 161
BcgI	CGANNNNNNTGC	3 cut(s)	33, 1110, 1144
BcoDI	GTCTC	5 cut(s)	49, 617, 1216, 1868, 1997
BfaI	CTAG	3 cut(s)	867, 1881, 1940
BfmI	CTRYAG	5 cut(s)	349, 706, 945, 993, 1576
BfoI	RGCGCY	1 cut(s)	1972
BfrI	CTTAAG	1 cut(s)	245
BfuAI	ACCTGC	1 cut(s)	977
BglI	GCCNNNNNGGC	1 cut(s)	374
BglII	AGATCT	1 cut(s)	887
BlpI	GCTNAGC	2 cut(s)	1381, 1904
Bme18I	GGWCC	1 cut(s)	1465
BmeT110I	CYCGRG	1 cut(s)	1728
BmgBI	CACGTC	2 cut(s)	773, 1991
BmgT120I	GGNCC	4 cut(s)	34, 35, 1209, 1465
BmiI	GGNNCC	6 cut(s)	36, 978, 1346, 1534, 1970, 2070
BmrI	ACTGGG	4 cut(s)	714, 1537, 1792, 2044
BmsI	GCATC	1 cut(s)	580
BmuI	ACTGGG	4 cut(s)	714, 1537, 1792, 2044
BoxI	GACNNNNGTC	1 cut(s)	1100
BpiI	GAAGAC	2 cut(s)	1704, 1928
Bpu10I	CCTNAGC	1 cut(s)	1217
Bpu1102I	GCTNAGC	2 cut(s)	1381, 1904
Bpu14I	TTCGAA	1 cut(s)	1120
BpuEI	CTTGAG	2 cut(s)	584, 2021
Bsa29I	ATCGAT	1 cut(s)	423
BsaAI	YACGTR	1 cut(s)	1046
BsaHI	GRCGYC	4 cut(s)	80, 174, 1788, 1969
BsaI	GGTCTC	2 cut(s)	49, 617
BsaJI	CCNNGG	4 cut(s)	280, 582, 1645, 1748
BsaWI	WCCGGW	1 cut(s)	112
BsaXI	ACNNNNNCTCC	4 cut(s)	215, 245, 647, 677
Bsc4I	CCNNNNNNNGG	2 cut(s)	1218, 2034
Bse1I	ACTGG	7 cut(s)	31, 720, 1532, 1536, 1609, 1787, 2039
Bse21I	CCTNAGG	1 cut(s)	387
Bse3DI	GCAATG	1 cut(s)	1176
BseCI	ATCGAT	1 cut(s)	423
BseDI	CCNNGG	4 cut(s)	280, 582, 1645, 1748
BseGI	GGATG	1 cut(s)	1947
BseLI	CCNNNNNNNGG	2 cut(s)	1218, 2034
BseMI	GCAATG	1 cut(s)	1176
BseNI	ACTGG	7 cut(s)	31, 720, 1532, 1536, 1609, 1787, 2039
BseRI	GAGGAG	5 cut(s)	101, 129, 917, 1234, 1746
BseSI	GKGCMC	3 cut(s)	38, 1349, 1703
BseXI	GCAGC	5 cut(s)	253, 931, 979, 1007, 1439
BsgI	GTGCAG	3 cut(s)	186, 955, 1560
Bsh1236I	CGCG	2 cut(s)	194, 261
Bsh1285I	CGRYCG	1 cut(s)	2119
BshFI	GGCC	4 cut(s)	36, 871, 1211, 1839
BshNI	GGYRCC	2 cut(s)	1344, 1968
BshVI	ATCGAT	1 cut(s)	423
BsiEI	CGRYCG	1 cut(s)	2119
BsiHKAI	GWGCWC	2 cut(s)	93, 1738
BsiHKCI	CYCGRG	1 cut(s)	1728
BsiSI	CCGG	2 cut(s)	83, 113
BslFI	GGGAC	1 cut(s)	1799
BslI	CCNNNNNNNGG	2 cut(s)	1218, 2034
BsmAI	GTCTC	5 cut(s)	49, 617, 1216, 1868, 1997
BsmFI	GGGAC	1 cut(s)	1799
BsmI	GAATGC	1 cut(s)	505
BsnI	GGCC	4 cut(s)	36, 871, 1211, 1839
Bso31I	GGTCTC	2 cut(s)	49, 617
BsoBI	CYCGRG	1 cut(s)	1728
Bsp119I	TTCGAA	1 cut(s)	1120
Bsp120I	GGGCCC	1 cut(s)	34
Bsp1286I	GDGCHC	6 cut(s)	38, 93, 1349, 1703, 1738, 1856
Bsp1407I	TGTACA	1 cut(s)	1175
Bsp143I	GATC	3 cut(s)	163, 887, 1559
Bsp1720I	GCTNAGC	2 cut(s)	1381, 1904
BspANI	GGCC	4 cut(s)	36, 871, 1211, 1839
BspDI	ATCGAT	1 cut(s)	423
BspFNI	CGCG	2 cut(s)	194, 261
BspLI	GGNNCC	6 cut(s)	36, 978, 1346, 1534, 1970, 2070
BspMAI	CTGCAG	2 cut(s)	949, 997
BspMI	ACCTGC	1 cut(s)	977
BspPI	GGATC	1 cut(s)	171
BspQI	GCTCTTC	1 cut(s)	1392
BspT104I	TTCGAA	1 cut(s)	1120
BspT107I	GGYRCC	2 cut(s)	1344, 1968
BspTI	CTTAAG	1 cut(s)	245
BspTNI	GGTCTC	2 cut(s)	49, 617
BsrDI	GCAATG	1 cut(s)	1176
BsrGI	TGTACA	1 cut(s)	1175
BsrI	ACTGG	7 cut(s)	31, 720, 1532, 1536, 1609, 1787, 2039
BssECI	CCNNGG	4 cut(s)	280, 582, 1645, 1748
BssMI	GATC	3 cut(s)	163, 887, 1559
BssNI	GRCGYC	4 cut(s)	80, 174, 1788, 1969
BssT1I	CCWWGG	3 cut(s)	582, 1645, 1748
Bst4CI	ACNGT	8 cut(s)	126, 207, 1102, 1279, 1291, 1318, 1441, 1710
Bst6I	CTCTTC	3 cut(s)	822, 894, 1392
BstACI	GRCGYC	4 cut(s)	80, 174, 1788, 1969
BstAFI	CTTAAG	1 cut(s)	245
BstAUI	TGTACA	1 cut(s)	1175
BstBAI	YACGTR	1 cut(s)	1046
BstBI	TTCGAA	1 cut(s)	1120
BstC8I	GCNNGC	8 cut(s)	201, 624, 1285, 1378, 1425, 1583, 1587, 1967
BstENI	CCTNNNNNAGG	1 cut(s)	1216
BstF5I	GGATG	1 cut(s)	1947
BstFNI	CGCG	2 cut(s)	194, 261
BstH2I	RGCGCY	1 cut(s)	1972
BstHHI	GCGC	1 cut(s)	1971
BstKTI	GATC	3 cut(s)	166, 890, 1562
BstMAI	GTCTC	5 cut(s)	49, 617, 1216, 1868, 1997
BstMBI	GATC	3 cut(s)	163, 887, 1559
BstMCI	CGRYCG	1 cut(s)	2119
BstNSI	RCATGY	2 cut(s)	626, 1158
BstPAI	GACNNNNGTC	1 cut(s)	1100
BstSFI	CTRYAG	5 cut(s)	349, 706, 945, 993, 1576
BstSLI	GKGCMC	3 cut(s)	38, 1349, 1703
BstUI	CGCG	2 cut(s)	194, 261
BstV1I	GCAGC	5 cut(s)	253, 931, 979, 1007, 1439
BstV2I	GAAGAC	2 cut(s)	1704, 1928
BstX2I	RGATCY	1 cut(s)	887
BstXI	CCANNNNNNTGG	1 cut(s)	55
BstYI	RGATCY	1 cut(s)	887
Bsu15I	ATCGAT	1 cut(s)	423
Bsu36I	CCTNAGG	1 cut(s)	387
BsuRI	GGCC	4 cut(s)	36, 871, 1211, 1839
BsuTUI	ATCGAT	1 cut(s)	423
BtgZI	GCGATG	1 cut(s)	91
BtrI	CACGTC	2 cut(s)	773, 1991
BtsCI	GGATG	1 cut(s)	1947
BtsIMutI	CAGTG	5 cut(s)	1075, 1287, 1543, 1616, 1780
BveI	ACCTGC	1 cut(s)	977
Cac8I	GCNNGC	8 cut(s)	201, 624, 1285, 1378, 1425, 1583, 1587, 1967
CaiI	CAGNNNCTG	2 cut(s)	992, 1433
CfoI	GCGC	1 cut(s)	1971
Cfr13I	GGNCC	4 cut(s)	34, 35, 1209, 1465
ClaI	ATCGAT	1 cut(s)	423
CseI	GACGC	3 cut(s)	88, 182, 1188
DinI	GGCGCC	1 cut(s)	1970
DpnI	GATC	3 cut(s)	165, 889, 1561
DpnII	GATC	3 cut(s)	163, 887, 1559
DraI	TTTAAA	1 cut(s)	1032
Eam1104I	CTCTTC	3 cut(s)	822, 894, 1392
EarI	CTCTTC	3 cut(s)	822, 894, 1392
Ecl136II	GAGCTC	1 cut(s)	91
Eco130I	CCWWGG	3 cut(s)	582, 1645, 1748
Eco147I	AGGCCT	1 cut(s)	871
Eco24I	GRGCYC	3 cut(s)	38, 93, 1856
Eco31I	GGTCTC	2 cut(s)	49, 617
Eco47I	GGWCC	1 cut(s)	1465
Eco53kI	GAGCTC	1 cut(s)	91
Eco57I	CTGAAG	2 cut(s)	837, 1051
Eco72I	CACGTG	1 cut(s)	1046
Eco81I	CCTNAGG	1 cut(s)	387
Eco88I	CYCGRG	1 cut(s)	1728
EcoICRI	GAGCTC	1 cut(s)	91
EcoNI	CCTNNNNNAGG	1 cut(s)	1216
EcoRI	GAATTC	1 cut(s)	1007
EcoT14I	CCWWGG	3 cut(s)	582, 1645, 1748
EcoT22I	ATGCAT	1 cut(s)	1721
EcoT38I	GRGCYC	3 cut(s)	38, 93, 1856
EgeI	GGCGCC	1 cut(s)	1970
EheI	GGCGCC	1 cut(s)	1970
ErhI	CCWWGG	3 cut(s)	582, 1645, 1748
FaqI	GGGAC	1 cut(s)	1799
FblI	GTMKAC	1 cut(s)	1320
FokI	GGATG	1 cut(s)	1934
FriOI	GRGCYC	3 cut(s)	38, 93, 1856
FspBI	CTAG	3 cut(s)	867, 1881, 1940
GlaI	GCGC	1 cut(s)	1970
HaeII	RGCGCY	1 cut(s)	1972
HaeIII	GGCC	4 cut(s)	36, 871, 1211, 1839
HapII	CCGG	2 cut(s)	83, 113
HgaI	GACGC	3 cut(s)	88, 182, 1188
HhaI	GCGC	1 cut(s)	1971
Hin1I	GRCGYC	4 cut(s)	80, 174, 1788, 1969
Hin6I	GCGC	1 cut(s)	1969
HinP1I	GCGC	1 cut(s)	1969
HincII	GTYRAC	2 cut(s)	747, 1444
HindII	GTYRAC	2 cut(s)	747, 1444
HindIII	AAGCTT	3 cut(s)	551, 1055, 1583
HinfI	GANTC	4 cut(s)	863, 1502, 1631, 2021
HpaII	CCGG	2 cut(s)	83, 113
HphI	GGTGA	4 cut(s)	127, 571, 929, 1172
Hpy166II	GTNNAC	9 cut(s)	254, 467, 579, 747, 1176, 1321, 1366, 1444, 1606
Hpy188III	TCNNGA	7 cut(s)	293, 885, 1271, 1563, 1873, 1940, 2072
Hpy8I	GTNNAC	9 cut(s)	254, 467, 579, 747, 1176, 1321, 1366, 1444, 1606
Hpy99I	CGWCG	3 cut(s)	11, 176, 1204
HpyAV	CCTTC	6 cut(s)	140, 214, 882, 1116, 1813, 2059
HpyCH4III	ACNGT	8 cut(s)	126, 207, 1102, 1279, 1291, 1318, 1441, 1710
HpyCH4IV	ACGT	6 cut(s)	213, 772, 1045, 1788, 1831, 1990
HpySE526I	ACGT	6 cut(s)	213, 772, 1045, 1788, 1831, 1990
Hsp92I	GRCGYC	4 cut(s)	80, 174, 1788, 1969
HspAI	GCGC	1 cut(s)	1969
KasI	GGCGCC	1 cut(s)	1968
Kzo9I	GATC	3 cut(s)	163, 887, 1559
LguI	GCTCTTC	1 cut(s)	1392
LmnI	GCTCC	6 cut(s)	88, 115, 1532, 1733, 1771, 1801
Lsp1109I	GCAGC	5 cut(s)	253, 931, 979, 1007, 1439
LweI	GCATC	1 cut(s)	580
MaeI	CTAG	3 cut(s)	867, 1881, 1940
MaeII	ACGT	6 cut(s)	213, 772, 1045, 1788, 1831, 1990
MaeIII	GTNAC	4 cut(s)	688, 722, 1543, 1789
MalI	GATC	3 cut(s)	165, 889, 1561
MboI	GATC	3 cut(s)	163, 887, 1559
MboII	GAAGA	9 cut(s)	130, 238, 522, 839, 911, 1103, 1379, 1704, 1928
MflI	RGATCY	1 cut(s)	887
MhlI	GDGCHC	6 cut(s)	38, 93, 1349, 1703, 1738, 1856
MluCI	AATT	7 cut(s)	628, 639, 679, 713, 894, 1007, 1740
Mly113I	GGCGCC	1 cut(s)	1969
MlyI	GAGTC	1 cut(s)	1496
MmeI	TCCRAC	3 cut(s)	72, 1084, 1182
Mph1103I	ATGCAT	1 cut(s)	1721
MroXI	GAANNNNTTC	2 cut(s)	786, 849
MseI	TTAA	5 cut(s)	246, 533, 1031, 1743, 1809
MslI	CAYNNNNRTG	3 cut(s)	474, 1044, 1451
MspA1I	CMGCKG	1 cut(s)	2030
MspCI	CTTAAG	1 cut(s)	245
MspI	CCGG	2 cut(s)	83, 113
Mva1269I	GAATGC	1 cut(s)	505
MvnI	CGCG	2 cut(s)	194, 261
NarI	GGCGCC	1 cut(s)	1969
NdeII	GATC	3 cut(s)	163, 887, 1559
NlaIV	GGNNCC	6 cut(s)	36, 978, 1346, 1534, 1970, 2070
NmuCI	GTSAC	1 cut(s)	1789
NsiI	ATGCAT	1 cut(s)	1721
NspI	RCATGY	2 cut(s)	626, 1158
NspV	TTCGAA	1 cut(s)	1120
OliI	CACNNNNGTG	1 cut(s)	1044
PaeI	GCATGC	1 cut(s)	626
PaeR7I	CTCGAG	1 cut(s)	1728
PceI	AGGCCT	1 cut(s)	871
PciI	ACATGT	1 cut(s)	1154
PciSI	GCTCTTC	1 cut(s)	1392
PctI	GAATGC	1 cut(s)	505
PdmI	GAANNNNTTC	2 cut(s)	786, 849
PfeI	GAWTC	3 cut(s)	863, 1631, 2021
PflFI	GACNNNGTC	1 cut(s)	176
PleI	GAGTC	1 cut(s)	1496
PluTI	GGCGCC	1 cut(s)	1972
PmaCI	CACGTG	1 cut(s)	1046
PmlI	CACGTG	1 cut(s)	1046
PpsI	GAGTC	1 cut(s)	1496
Ppu21I	YACGTR	1 cut(s)	1046
PscI	ACATGT	1 cut(s)	1154
PshAI	GACNNNNGTC	1 cut(s)	1100
Psp124BI	GAGCTC	1 cut(s)	93
PspCI	CACGTG	1 cut(s)	1046
PspN4I	GGNNCC	6 cut(s)	36, 978, 1346, 1534, 1970, 2070
PspOMI	GGGCCC	1 cut(s)	34
PspPI	GGNCC	4 cut(s)	34, 35, 1209, 1465
PspXI	VCTCGAGB	1 cut(s)	1728
PstI	CTGCAG	2 cut(s)	949, 997
PstNI	CAGNNNCTG	2 cut(s)	992, 1433
PsuI	RGATCY	1 cut(s)	887
PsyI	GACNNNGTC	1 cut(s)	176
RseI	CAYNNNNRTG	3 cut(s)	474, 1044, 1451
SacI	GAGCTC	1 cut(s)	93
SapI	GCTCTTC	1 cut(s)	1392
SaqAI	TTAA	5 cut(s)	246, 533, 1031, 1743, 1809
Sau3AI	GATC	3 cut(s)	163, 887, 1559
Sau96I	GGNCC	4 cut(s)	34, 35, 1209, 1465
SchI	GAGTC	1 cut(s)	1496
SduI	GDGCHC	6 cut(s)	38, 93, 1349, 1703, 1738, 1856
SfaNI	GCATC	1 cut(s)	580
SfcI	CTRYAG	5 cut(s)	349, 706, 945, 993, 1576
SfoI	GGCGCC	1 cut(s)	1970
Sfr274I	CTCGAG	1 cut(s)	1728
SfuI	TTCGAA	1 cut(s)	1120
SinI	GGWCC	1 cut(s)	1465
SlaI	CTCGAG	1 cut(s)	1728
SmiMI	CAYNNNNRTG	3 cut(s)	474, 1044, 1451
SmlI	CTYRAG	4 cut(s)	245, 599, 1728, 2000
SmoI	CTYRAG	4 cut(s)	245, 599, 1728, 2000
SphI	GCATGC	1 cut(s)	626
Sse9I	AATT	7 cut(s)	628, 639, 679, 713, 894, 1007, 1740
SseBI	AGGCCT	1 cut(s)	871
SspDI	GGCGCC	1 cut(s)	1968
SspI	AATATT	1 cut(s)	1813
SspMI	CTAG	3 cut(s)	867, 1881, 1940
SstI	GAGCTC	1 cut(s)	93
StuI	AGGCCT	1 cut(s)	871
StyI	CCWWGG	3 cut(s)	582, 1645, 1748
TaaI	ACNGT	8 cut(s)	126, 207, 1102, 1279, 1291, 1318, 1441, 1710
TaiI	ACGT	6 cut(s)	216, 775, 1048, 1791, 1834, 1993
TaqI	TCGA	6 cut(s)	209, 423, 1120, 1558, 1729, 1872
TaqII	GACCGA	1 cut(s)	2133
TasI	AATT	7 cut(s)	628, 639, 679, 713, 894, 1007, 1740
TatI	WGTACW	1 cut(s)	1175
TauI	GCSGC	9 cut(s)	23, 62, 65, 68, 71, 241, 261, 348, 2030
TfiI	GAWTC	3 cut(s)	863, 1631, 2021
Tru1I	TTAA	5 cut(s)	246, 533, 1031, 1743, 1809
Tru9I	TTAA	5 cut(s)	246, 533, 1031, 1743, 1809
TscAI	CASTG	5 cut(s)	1075, 1294, 1543, 1616, 1787
TseFI	GTSAC	1 cut(s)	1789
TseI	GCWGC	5 cut(s)	241, 944, 992, 995, 1427
Tsp45I	GTSAC	1 cut(s)	1789
TspDTI	ATGAA	9 cut(s)	539, 587, 795, 813, 858, 1218, 1315, 1424, 1704
TspGWI	ACGGA	1 cut(s)	989
TspRI	CASTG	5 cut(s)	1075, 1294, 1543, 1616, 1787
Tth111I	GACNNNGTC	1 cut(s)	176
Vha464I	CTTAAG	1 cut(s)	245
VpaK11BI	GGWCC	1 cut(s)	1465
XagI	CCTNNNNNAGG	1 cut(s)	1216
XapI	RAATTY	2 cut(s)	713, 1007
XbaI	TCTAGA	1 cut(s)	1939
XceI	RCATGY	2 cut(s)	626, 1158
XhoI	CTCGAG	1 cut(s)	1728
XmiI	GTMKAC	1 cut(s)	1320
XmnI	GAANNNNTTC	2 cut(s)	786, 849
XspI	CTAG	3 cut(s)	867, 1881, 1940
ZraI	GACGTC	1 cut(s)	1789
Zsp2I	ATGCAT	1 cut(s)	1721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.