Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0004194

Physical Location & Seq

Genomic Coordinates Reverse (-)

47102983 .. 47109974

Gene Structure 6992 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00199830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGGGCGACGGCACTCCTGCCCCAACTGGGCCGGGCCCAGAGGTCTCCAGCGGCGTGGCGGCGGCGGCGGCTGAGAGGACGCCGGAGGAGCTCGTAGCCAAGTCCATCGCTCCGGTGAAGAAACAGTACCTTCGCCCTCCTCCCGTCAGACCTTGCTCTAAGGATCAAAACGACGCCGTCTGTGAGGGCAGCGCAAAGCCTGCCCAGTCTAACGTAGTGAAGGAGAAGAAGTCCAAGCGGCAGCTCAAGCGTGAACGCCGCGAGGTAGCAAAGAAAGGAGATGTTAGTCGGTGTCCATATAATGATAAGTGCCGCTACAGCCACGACATAGAAGCCTATAAGGCTCAGAAACCTGAGGATATAGAGGGAGAATGTCCTTTTATGGCTATCGATGAGCCATGCCCTTATGGTTTGTCATGTAGGTTCTTGGGTACACATAAAAATGGCATTGCAGATGGAGATGTGAATGCTCGTAGAAGAAGCTCTGAGATGAATGGATTAAGCAAAAATGTTCAAAAGCTTTTGTGGAAAAATAAAATGAAGTTCACCAAGGCCGATGCCAAACTCAAGGCTCTTGGTCTCATGGGGCATGCCAATTCAAAAATAATTGCCATGTCAAAGGAGAATGGGTCGGTGGTTTCAATGAATTGTGATGTTACTGATGGAAATGGCTGTAGTGAATTTCCCAGTAACACAGATGATAAGGTGGAGTTGACTGCTGAAATAAAAGAAGAGGGACGTGTTGATGAAAAGTTTCCATCTGGTGAAATGGAGTTGTCTGCTGAAGTGAAAGAAGAGGGAGGTGCTAATGAAACATTTCCATCTGACGATTCTAGGCCTTCAAAGAAGTCAAGATCTGTAATTGAAGAGGAGTTTGGTTCTGGTGAAGCAGATTATGGAGGTGTGAGAGCTGCAGGAAAAGTTGTTGGGCAAACCTGCACGGAACCTGAACCAGAAGCTGCAGCTAACATTGAATTCTCAGAGAATGATAGAAGTTTAAAGACACACCCACGTGAAAAGAAGCTTATTGACTTCAGAGAAAAGTTGTACCTTGCACCTCTGACCACAGTCGGAAATCTTCCATTTCGAAGGGTTTGCAAAGGTTTAGGGGCAGATATAACATGTGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCGTCGGAATGGGCCTTGCTGAGGAGACATTCATCTGAGGATTTGTTTGGCGTTCAGATATGTGGGGCATATCCAGACACCGTAGCACGCACTGTTGAACTTATAGAACAGGAATGTACTGTAGACTTCATTGATATAAATATGGGGTGCCCAATAGATATCGTTGTGAACAAGGGTGCTGGCTCAGCTCTTCTAACAAAACCAATGCGAATGAAAGGCATTGTGCAAGCAGCTTCTGGTACAGTTGACATTCCTATGACTATCAAGGTCCGCACAGCCTATTTTGAGGGGAAAAATCGCATTGACTCACTTATTGCTGACATGGGCAACTGGGGAGCCAGTGCAGTAACAATACATGGTCGATCACGACAGCAACGCTATCAAAATTTCTGCAAGCTTTTATGGGCAGATTGGGAATATGTGTACCAGTGTGCTAAAAAAGCCCCTGATTCTTTGCAAGTCCTTGGAAATGGAGATGTATTTTCGTATGTAGATTGGAACAAGCACAAGGCTGAGTGCCCTGAACTGTCTTCATGCATGATTGCTCGAGGAGCACTAATTAAGCCTTGGATTTTTACAGAAATAAAGGAGCAGAGGCACTGGGACATCACTTCTGGGGAGCGATTTAATATTTTGAAGGATTATGTACGTTGTGGCCTTGAACATTGGGGGTCTGACACAAAAGGTGTTGAGACAACTAGGCGTTTTCTGTTAGAATGGCTTAGCTACACTTGTAGATATGTACCTGTTGGTCTTCTAGATGTCATCCCACAACAACTGAACTGGCGCCCACCATCTTATTTTGGACGTGATGACCTTGAGACAGTAATGGCTTCTGATTCTGCCGCTGACTGGGTTAGGCTCTCTGAGATGTTGCTTGGGAAGGTTCCAGATGGTTTCACATTTGCACCAAAGCATAAATCCAATGCTTACGACCGAGCAGAAAACGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002943 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016491 GO:0016627 GO:0017150 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0055114 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dus zf-CCCH

Protein Analysis

705

Amino Acids

78.08

Weight (kDa)

6.31

Isoelectric Point (pI)

43.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCCH_DUS3L	PF25585	96 - 116	3.8e-08	DUS3L CCCH zinc finger domain
zf-CCCH_DUS3L	PF25585	117 - 148	2.7e-07	DUS3L CCCH zinc finger domain
Dus	PF01207	351 - 610	8.7e-46	Dihydrouridine synthase (Dus)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38890
fragaria_vesca	FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690 FvH4_2g10690
malus_domestica	MD05G1054400.v1.1 MD10G1062000.v1.1
prunus_persica	Prupe.8G082800_v2.0.a1
pyrus_communis	pycom10g04770
rosa_chinensis	RchiOBHm_Chr6g0268371 RchiOBHm_Chr6g0268451 RchiOBHm_Chr6g0268461 RchiOBHm_Chr6g0271651 RchiOBHm_Chr6g0271661
rosa_laevigata	RLG00000013910
rosa_multiflora	Rmu_sc0002404.1_g000009 Rmu_sc0002404.1_g000017 Rmu_sc0007949.1_g000004 Rmu_sc0008184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00199830
rosa_rugosa	Rorug06G0041000 Rorug06G0042100
rosa_samantha	Rh6AG161000 Rh6BG165400 Rh6CG152700 Rh6CG159400 Rh6DG151200
rosa_wichuraiana	Rw6G013920 Rw6G013960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	944
AccB1I	GGYRCC	2 cut(s)	1311, 1950
AccI	GTMKAC	1 cut(s)	1287
AccII	CGCG	1 cut(s)	261
AclWI	GGATC	1 cut(s)	171
AcsI	RAATTY	3 cut(s)	680, 974, 1549
AcuI	CTGAAG	2 cut(s)	804, 1018
AcvI	CACGTG	1 cut(s)	1013
AcyI	GRCGYC	3 cut(s)	80, 174, 1951
AfaI	GTAC	9 cut(s)	128, 433, 1049, 1144, 1282, 1405, 1589, 1812, 1908
AfiI	CCNNNNNNNGG	3 cut(s)	27, 1185, 2016
AflIII	ACRYGT	2 cut(s)	739, 1121
AgsI	TTSAA	9 cut(s)	515, 600, 642, 843, 866, 974, 1262, 1801, 1826
AjiI	CACGTC	2 cut(s)	740, 1973
AjuI	GAANNNNNNNTTGG	2 cut(s)	858, 890
AleI	CACNNNNGTG	1 cut(s)	1011
Alw21I	GWGCWC	2 cut(s)	93, 1720
Alw26I	GTCTC	5 cut(s)	49, 584, 1183, 1850, 1979
AlwI	GGATC	1 cut(s)	171
AlwNI	CAGNNNCTG	2 cut(s)	959, 1400
Ama87I	CYCGRG	1 cut(s)	1710
AoxI	GGCC	6 cut(s)	29, 34, 552, 836, 1176, 1819
ApaI	GGGCCC	1 cut(s)	38
ApeKI	GCWGC	6 cut(s)	189, 241, 911, 959, 962, 1394
ApoI	RAATTY	3 cut(s)	680, 974, 1549
Asp700I	GAANNNNTTC	2 cut(s)	753, 816
AspLEI	GCGC	2 cut(s)	194, 1953
AspS9I	GGNCC	5 cut(s)	29, 34, 35, 1176, 1432
AsuC2I	CCSGG	1 cut(s)	33
AsuHPI	GGTGA	5 cut(s)	127, 538, 776, 896, 1139
AsuII	TTCGAA	1 cut(s)	1087
AvaI	CYCGRG	1 cut(s)	1710
AvaII	GGWCC	1 cut(s)	1432
AxyI	CCTNAGG	1 cut(s)	354
BaeGI	GKGCMC	3 cut(s)	38, 1316, 1685
BanI	GGYRCC	2 cut(s)	1311, 1950
BanII	GRGCYC	2 cut(s)	38, 93
BarI	GAAGNNNNNNTAC	2 cut(s)	110, 142
BbrPI	CACGTG	1 cut(s)	1013
BbsI	GAAGAC	2 cut(s)	1686, 1910
Bbv12I	GWGCWC	2 cut(s)	93, 1720
BbvCI	CCTCAGC	1 cut(s)	1184
BbvI	GCAGC	6 cut(s)	201, 253, 898, 946, 974, 1406
BccI	CCATC	7 cut(s)	113, 449, 656, 766, 829, 1966, 2051
BceAI	ACGGC	2 cut(s)	25, 161
BcgI	CGANNNNNNTGC	2 cut(s)	1077, 1111
BcnI	CCSGG	1 cut(s)	33
BcoDI	GTCTC	5 cut(s)	49, 584, 1183, 1850, 1979
BfaI	CTAG	3 cut(s)	834, 1863, 1922
BfmI	CTRYAG	5 cut(s)	316, 673, 912, 960, 1284
BfoI	RGCGCY	1 cut(s)	1954
BfuAI	ACCTGC	1 cut(s)	944
BglI	GCCNNNNNGGC	1 cut(s)	341
BglII	AGATCT	1 cut(s)	854
BlpI	GCTNAGC	2 cut(s)	1348, 1886
Bme1390I	CCNGG	1 cut(s)	33
Bme18I	GGWCC	1 cut(s)	1432
BmeT110I	CYCGRG	1 cut(s)	1710
BmgBI	CACGTC	2 cut(s)	740, 1973
BmgT120I	GGNCC	5 cut(s)	29, 34, 35, 1176, 1432
BmiI	GGNNCC	6 cut(s)	36, 945, 1313, 1501, 1952, 2052
BmrFI	CCNGG	1 cut(s)	33
BmrI	ACTGGG	6 cut(s)	36, 199, 681, 1504, 1774, 2026
BmsI	GCATC	1 cut(s)	547
BmuI	ACTGGG	6 cut(s)	36, 199, 681, 1504, 1774, 2026
BoxI	GACNNNNGTC	1 cut(s)	1067
BpiI	GAAGAC	2 cut(s)	1686, 1910
BpmI	CTGGAG	1 cut(s)	31
Bpu10I	CCTNAGC	1 cut(s)	1184
Bpu1102I	GCTNAGC	2 cut(s)	1348, 1886
Bpu14I	TTCGAA	1 cut(s)	1087
BpuEI	CTTGAG	3 cut(s)	230, 551, 2003
BpuMI	CCSGG	1 cut(s)	33
Bsa29I	ATCGAT	1 cut(s)	390
BsaAI	YACGTR	1 cut(s)	1013
BsaHI	GRCGYC	3 cut(s)	80, 174, 1951
BsaI	GGTCTC	2 cut(s)	49, 584
BsaJI	CCNNGG	3 cut(s)	549, 1627, 1730
BsaWI	WCCGGW	1 cut(s)	112
BsaXI	ACNNNNNCTCC	4 cut(s)	215, 245, 614, 644
Bsc4I	CCNNNNNNNGG	3 cut(s)	27, 1185, 2016
Bse1I	ACTGG	9 cut(s)	31, 205, 687, 1499, 1503, 1591, 1769, 1952, 2021
Bse21I	CCTNAGG	1 cut(s)	354
Bse3DI	GCAATG	2 cut(s)	447, 1143
BseCI	ATCGAT	1 cut(s)	390
BseDI	CCNNGG	3 cut(s)	549, 1627, 1730
BseGI	GGATG	1 cut(s)	1929
BseLI	CCNNNNNNNGG	3 cut(s)	27, 1185, 2016
BseMI	GCAATG	2 cut(s)	447, 1143
BseNI	ACTGG	9 cut(s)	31, 205, 687, 1499, 1503, 1591, 1769, 1952, 2021
BseRI	GAGGAG	5 cut(s)	101, 129, 884, 1201, 1728
BseSI	GKGCMC	3 cut(s)	38, 1316, 1685
BseXI	GCAGC	6 cut(s)	201, 253, 898, 946, 974, 1406
BsgI	GTGCAG	2 cut(s)	922, 1527
Bsh1236I	CGCG	1 cut(s)	261
Bsh1285I	CGRYCG	1 cut(s)	2101
BshFI	GGCC	6 cut(s)	31, 36, 554, 838, 1178, 1821
BshNI	GGYRCC	2 cut(s)	1311, 1950
BshVI	ATCGAT	1 cut(s)	390
BsiEI	CGRYCG	1 cut(s)	2101
BsiHKAI	GWGCWC	2 cut(s)	93, 1720
BsiHKCI	CYCGRG	1 cut(s)	1710
BsiSI	CCGG	3 cut(s)	32, 83, 113
BslFI	GGGAC	2 cut(s)	750, 1781
BslI	CCNNNNNNNGG	3 cut(s)	27, 1185, 2016
BsmAI	GTCTC	5 cut(s)	49, 584, 1183, 1850, 1979
BsmFI	GGGAC	2 cut(s)	750, 1781
BsmI	GAATGC	1 cut(s)	472
BsnI	GGCC	6 cut(s)	31, 36, 554, 838, 1178, 1821
Bso31I	GGTCTC	2 cut(s)	49, 584
BsoBI	CYCGRG	1 cut(s)	1710
Bsp119I	TTCGAA	1 cut(s)	1087
Bsp120I	GGGCCC	1 cut(s)	34
Bsp1286I	GDGCHC	5 cut(s)	38, 93, 1316, 1685, 1720
Bsp1407I	TGTACA	1 cut(s)	1142
Bsp143I	GATC	3 cut(s)	163, 854, 1526
Bsp1720I	GCTNAGC	2 cut(s)	1348, 1886
BspANI	GGCC	6 cut(s)	31, 36, 554, 838, 1178, 1821
BspDI	ATCGAT	1 cut(s)	390
BspFNI	CGCG	1 cut(s)	261
BspLI	GGNNCC	6 cut(s)	36, 945, 1313, 1501, 1952, 2052
BspMAI	CTGCAG	2 cut(s)	916, 964
BspMI	ACCTGC	1 cut(s)	944
BspPI	GGATC	1 cut(s)	171
BspQI	GCTCTTC	1 cut(s)	1359
BspT104I	TTCGAA	1 cut(s)	1087
BspT107I	GGYRCC	2 cut(s)	1311, 1950
BspTNI	GGTCTC	2 cut(s)	49, 584
BsrDI	GCAATG	2 cut(s)	447, 1143
BsrGI	TGTACA	1 cut(s)	1142
BsrI	ACTGG	9 cut(s)	31, 205, 687, 1499, 1503, 1591, 1769, 1952, 2021
BssECI	CCNNGG	3 cut(s)	549, 1627, 1730
BssMI	GATC	3 cut(s)	163, 854, 1526
BssNI	GRCGYC	3 cut(s)	80, 174, 1951
BssT1I	CCWWGG	3 cut(s)	549, 1627, 1730
Bst4CI	ACNGT	8 cut(s)	126, 1069, 1246, 1258, 1285, 1408, 1692, 1990
Bst6I	CTCTTC	4 cut(s)	726, 789, 861, 1359
BstACI	GRCGYC	3 cut(s)	80, 174, 1951
BstAPI	GCANNNNNTGC	1 cut(s)	200
BstAUI	TGTACA	1 cut(s)	1142
BstBAI	YACGTR	1 cut(s)	1013
BstBI	TTCGAA	1 cut(s)	1087
BstC8I	GCNNGC	6 cut(s)	201, 591, 1252, 1345, 1392, 1559
BstENI	CCTNNNNNAGG	1 cut(s)	1183
BstF5I	GGATG	1 cut(s)	1929
BstFNI	CGCG	1 cut(s)	261
BstH2I	RGCGCY	1 cut(s)	1954
BstHHI	GCGC	2 cut(s)	194, 1953
BstKTI	GATC	3 cut(s)	166, 857, 1529
BstMAI	GTCTC	5 cut(s)	49, 584, 1183, 1850, 1979
BstMBI	GATC	3 cut(s)	163, 854, 1526
BstMCI	CGRYCG	1 cut(s)	2101
BstNSI	RCATGY	2 cut(s)	593, 1125
BstPAI	GACNNNNGTC	1 cut(s)	1067
BstSCI	CCNGG	1 cut(s)	31
BstSFI	CTRYAG	5 cut(s)	316, 673, 912, 960, 1284
BstSLI	GKGCMC	3 cut(s)	38, 1316, 1685
BstUI	CGCG	1 cut(s)	261
BstV1I	GCAGC	6 cut(s)	201, 253, 898, 946, 974, 1406
BstV2I	GAAGAC	2 cut(s)	1686, 1910
BstX2I	RGATCY	1 cut(s)	854
BstXI	CCANNNNNNTGG	1 cut(s)	55
BstYI	RGATCY	1 cut(s)	854
Bsu15I	ATCGAT	1 cut(s)	390
Bsu36I	CCTNAGG	1 cut(s)	354
BsuRI	GGCC	6 cut(s)	31, 36, 554, 838, 1178, 1821
BsuTUI	ATCGAT	1 cut(s)	390
BtgZI	GCGATG	1 cut(s)	91
BtrI	CACGTC	2 cut(s)	740, 1973
BtsCI	GGATG	1 cut(s)	1929
BtsIMutI	CAGTG	4 cut(s)	1254, 1510, 1598, 1762
BveI	ACCTGC	1 cut(s)	944
Cac8I	GCNNGC	6 cut(s)	201, 591, 1252, 1345, 1392, 1559
CaiI	CAGNNNCTG	2 cut(s)	959, 1400
CfoI	GCGC	2 cut(s)	194, 1953
Cfr13I	GGNCC	5 cut(s)	29, 34, 35, 1176, 1432
ClaI	ATCGAT	1 cut(s)	390
CseI	GACGC	3 cut(s)	88, 182, 1155
Csp6I	GTAC	9 cut(s)	127, 432, 1048, 1143, 1281, 1404, 1588, 1811, 1907
CviQI	GTAC	9 cut(s)	127, 432, 1048, 1143, 1281, 1404, 1588, 1811, 1907
DinI	GGCGCC	1 cut(s)	1952
DpnI	GATC	3 cut(s)	165, 856, 1528
DpnII	GATC	3 cut(s)	163, 854, 1526
DraI	TTTAAA	1 cut(s)	999
Eam1104I	CTCTTC	4 cut(s)	726, 789, 861, 1359
EarI	CTCTTC	4 cut(s)	726, 789, 861, 1359
Ecl136II	GAGCTC	1 cut(s)	91
Eco130I	CCWWGG	3 cut(s)	549, 1627, 1730
Eco147I	AGGCCT	1 cut(s)	838
Eco24I	GRGCYC	2 cut(s)	38, 93
Eco31I	GGTCTC	2 cut(s)	49, 584
Eco32I	GATATC	1 cut(s)	1324
Eco47I	GGWCC	1 cut(s)	1432
Eco53kI	GAGCTC	1 cut(s)	91
Eco57I	CTGAAG	2 cut(s)	804, 1018
Eco72I	CACGTG	1 cut(s)	1013
Eco81I	CCTNAGG	1 cut(s)	354
Eco88I	CYCGRG	1 cut(s)	1710
EcoICRI	GAGCTC	1 cut(s)	91
EcoNI	CCTNNNNNAGG	1 cut(s)	1183
EcoRI	GAATTC	1 cut(s)	974
EcoRV	GATATC	1 cut(s)	1324
EcoT14I	CCWWGG	3 cut(s)	549, 1627, 1730
EcoT22I	ATGCAT	1 cut(s)	1703
EcoT38I	GRGCYC	2 cut(s)	38, 93
EgeI	GGCGCC	1 cut(s)	1952
EheI	GGCGCC	1 cut(s)	1952
ErhI	CCWWGG	3 cut(s)	549, 1627, 1730
FaqI	GGGAC	2 cut(s)	750, 1781
FblI	GTMKAC	1 cut(s)	1287
FokI	GGATG	1 cut(s)	1916
FriOI	GRGCYC	2 cut(s)	38, 93
FspBI	CTAG	3 cut(s)	834, 1863, 1922
GlaI	GCGC	2 cut(s)	193, 1952
GsuI	CTGGAG	1 cut(s)	31
HaeII	RGCGCY	1 cut(s)	1954
HaeIII	GGCC	6 cut(s)	31, 36, 554, 838, 1178, 1821
HapII	CCGG	3 cut(s)	32, 83, 113
HgaI	GACGC	3 cut(s)	88, 182, 1155
HhaI	GCGC	2 cut(s)	194, 1953
Hin1I	GRCGYC	3 cut(s)	80, 174, 1951
Hin6I	GCGC	2 cut(s)	192, 1951
HinP1I	GCGC	2 cut(s)	192, 1951
HincII	GTYRAC	2 cut(s)	714, 1411
HindII	GTYRAC	2 cut(s)	714, 1411
HindIII	AAGCTT	3 cut(s)	518, 1022, 1559
HinfI	GANTC	4 cut(s)	830, 1469, 1613, 2003
HpaII	CCGG	3 cut(s)	32, 83, 113
HphI	GGTGA	5 cut(s)	127, 538, 776, 896, 1139
Hpy166II	GTNNAC	9 cut(s)	254, 434, 546, 714, 1143, 1288, 1333, 1411, 1588
Hpy188III	TCNNGA	5 cut(s)	852, 1238, 1530, 1922, 2054
Hpy8I	GTNNAC	9 cut(s)	254, 434, 546, 714, 1143, 1288, 1333, 1411, 1588
Hpy99I	CGWCG	3 cut(s)	11, 176, 1171
HpyAV	CCTTC	6 cut(s)	140, 214, 849, 1083, 1795, 2041
HpyCH4III	ACNGT	8 cut(s)	126, 1069, 1246, 1258, 1285, 1408, 1692, 1990
HpyCH4IV	ACGT	5 cut(s)	213, 739, 1012, 1813, 1972
HpySE526I	ACGT	5 cut(s)	213, 739, 1012, 1813, 1972
Hsp92I	GRCGYC	3 cut(s)	80, 174, 1951
HspAI	GCGC	2 cut(s)	192, 1951
KasI	GGCGCC	1 cut(s)	1950
Kzo9I	GATC	3 cut(s)	163, 854, 1526
LguI	GCTCTTC	1 cut(s)	1359
LmnI	GCTCC	6 cut(s)	88, 115, 1499, 1715, 1753, 1783
Lsp1109I	GCAGC	6 cut(s)	201, 253, 898, 946, 974, 1406
LweI	GCATC	1 cut(s)	547
MaeI	CTAG	3 cut(s)	834, 1863, 1922
MaeII	ACGT	5 cut(s)	213, 739, 1012, 1813, 1972
MaeIII	GTNAC	3 cut(s)	655, 689, 1510
MalI	GATC	3 cut(s)	165, 856, 1528
MboI	GATC	3 cut(s)	163, 854, 1526
MflI	RGATCY	1 cut(s)	854
MhlI	GDGCHC	5 cut(s)	38, 93, 1316, 1685, 1720
MluCI	AATT	8 cut(s)	595, 606, 646, 680, 861, 974, 1549, 1722
Mly113I	GGCGCC	1 cut(s)	1951
MlyI	GAGTC	1 cut(s)	1463
MmeI	TCCRAC	2 cut(s)	1051, 1149
Mph1103I	ATGCAT	1 cut(s)	1703
MroXI	GAANNNNTTC	2 cut(s)	753, 816
MseI	TTAA	4 cut(s)	500, 998, 1725, 1791
MslI	CAYNNNNRTG	3 cut(s)	441, 1011, 1418
MspA1I	CMGCKG	2 cut(s)	51, 2012
MspI	CCGG	3 cut(s)	32, 83, 113
MspR9I	CCNGG	1 cut(s)	33
Mva1269I	GAATGC	1 cut(s)	472
MvnI	CGCG	1 cut(s)	261
NarI	GGCGCC	1 cut(s)	1951
NciI	CCSGG	1 cut(s)	33
NdeII	GATC	3 cut(s)	163, 854, 1526
NlaIV	GGNNCC	6 cut(s)	36, 945, 1313, 1501, 1952, 2052
NsiI	ATGCAT	1 cut(s)	1703
NspI	RCATGY	2 cut(s)	593, 1125
NspV	TTCGAA	1 cut(s)	1087
OliI	CACNNNNGTG	1 cut(s)	1011
PaeI	GCATGC	1 cut(s)	593
PaeR7I	CTCGAG	1 cut(s)	1710
PceI	AGGCCT	1 cut(s)	838
PciI	ACATGT	1 cut(s)	1121
PciSI	GCTCTTC	1 cut(s)	1359
PctI	GAATGC	1 cut(s)	472
PdmI	GAANNNNTTC	2 cut(s)	753, 816
PfeI	GAWTC	3 cut(s)	830, 1613, 2003
PflFI	GACNNNGTC	1 cut(s)	176
PleI	GAGTC	1 cut(s)	1463
PluTI	GGCGCC	1 cut(s)	1954
PmaCI	CACGTG	1 cut(s)	1013
PmlI	CACGTG	1 cut(s)	1013
PpsI	GAGTC	1 cut(s)	1463
Ppu21I	YACGTR	1 cut(s)	1013
PscI	ACATGT	1 cut(s)	1121
PshAI	GACNNNNGTC	1 cut(s)	1067
Psp124BI	GAGCTC	1 cut(s)	93
PspCI	CACGTG	1 cut(s)	1013
PspN4I	GGNNCC	6 cut(s)	36, 945, 1313, 1501, 1952, 2052
PspOMI	GGGCCC	1 cut(s)	34
PspPI	GGNCC	5 cut(s)	29, 34, 35, 1176, 1432
PspXI	VCTCGAGB	1 cut(s)	1710
PstI	CTGCAG	2 cut(s)	916, 964
PstNI	CAGNNNCTG	2 cut(s)	959, 1400
PsuI	RGATCY	1 cut(s)	854
PsyI	GACNNNGTC	1 cut(s)	176
RsaI	GTAC	9 cut(s)	128, 433, 1049, 1144, 1282, 1405, 1589, 1812, 1908
RsaNI	GTAC	9 cut(s)	127, 432, 1048, 1143, 1281, 1404, 1588, 1811, 1907
RseI	CAYNNNNRTG	3 cut(s)	441, 1011, 1418
SacI	GAGCTC	1 cut(s)	93
SapI	GCTCTTC	1 cut(s)	1359
SaqAI	TTAA	4 cut(s)	500, 998, 1725, 1791
Sau3AI	GATC	3 cut(s)	163, 854, 1526
Sau96I	GGNCC	5 cut(s)	29, 34, 35, 1176, 1432
SchI	GAGTC	1 cut(s)	1463
ScrFI	CCNGG	1 cut(s)	33
SduI	GDGCHC	5 cut(s)	38, 93, 1316, 1685, 1720
SfaNI	GCATC	1 cut(s)	547
SfcI	CTRYAG	5 cut(s)	316, 673, 912, 960, 1284
SfoI	GGCGCC	1 cut(s)	1952
Sfr274I	CTCGAG	1 cut(s)	1710
SfuI	TTCGAA	1 cut(s)	1087
SinI	GGWCC	1 cut(s)	1432
SlaI	CTCGAG	1 cut(s)	1710
SmiMI	CAYNNNNRTG	3 cut(s)	441, 1011, 1418
SmlI	CTYRAG	4 cut(s)	245, 566, 1710, 1982
SmoI	CTYRAG	4 cut(s)	245, 566, 1710, 1982
SphI	GCATGC	1 cut(s)	593
Sse9I	AATT	8 cut(s)	595, 606, 646, 680, 861, 974, 1549, 1722
SseBI	AGGCCT	1 cut(s)	838
SspDI	GGCGCC	1 cut(s)	1950
SspI	AATATT	1 cut(s)	1795
SspMI	CTAG	3 cut(s)	834, 1863, 1922
SstI	GAGCTC	1 cut(s)	93
StuI	AGGCCT	1 cut(s)	838
StyD4I	CCNGG	1 cut(s)	31
StyI	CCWWGG	3 cut(s)	549, 1627, 1730
TaaI	ACNGT	8 cut(s)	126, 1069, 1246, 1258, 1285, 1408, 1692, 1990
TaiI	ACGT	5 cut(s)	216, 742, 1015, 1816, 1975
TaqI	TCGA	4 cut(s)	390, 1087, 1525, 1711
TaqII	GACCGA	1 cut(s)	2115
TasI	AATT	8 cut(s)	595, 606, 646, 680, 861, 974, 1549, 1722
TatI	WGTACW	2 cut(s)	1142, 1280
TauI	GCSGC	9 cut(s)	54, 62, 65, 68, 71, 241, 261, 315, 2012
TfiI	GAWTC	3 cut(s)	830, 1613, 2003
Tru1I	TTAA	4 cut(s)	500, 998, 1725, 1791
Tru9I	TTAA	4 cut(s)	500, 998, 1725, 1791
TscAI	CASTG	4 cut(s)	1261, 1510, 1598, 1769
TseI	GCWGC	6 cut(s)	189, 241, 911, 959, 962, 1394
TspDTI	ATGAA	9 cut(s)	506, 554, 659, 762, 825, 1185, 1282, 1391, 1686
TspGWI	ACGGA	1 cut(s)	956
TspRI	CASTG	4 cut(s)	1261, 1510, 1598, 1769
Tth111I	GACNNNGTC	1 cut(s)	176
VpaK11BI	GGWCC	1 cut(s)	1432
XagI	CCTNNNNNAGG	1 cut(s)	1183
XapI	RAATTY	3 cut(s)	680, 974, 1549
XbaI	TCTAGA	1 cut(s)	1921
XceI	RCATGY	2 cut(s)	593, 1125
XhoI	CTCGAG	1 cut(s)	1710
XmiI	GTMKAC	1 cut(s)	1287
XmnI	GAANNNNTTC	2 cut(s)	753, 816
XspI	CTAG	3 cut(s)	834, 1863, 1922
Zsp2I	ATGCAT	1 cut(s)	1703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.