Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004310

Physical Location & Seq

Genomic Coordinates Forward (+)

10058044 .. 10069598

Gene Structure 11555 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1058100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGGCCTCGTCCTCACGTGCCCGCAGCAGCTCGCTTTTCTCCTACCGGAAGCCATCCAGTCCCTACTCCTCCACCTCCTCGTCTTCCGCGTTGATCAACGGTCGGATGATTCCGCGCTCCTGCTCGACCTCGGCCTCCTCGTTCTACAACTCGGGAGGTGGACTCGGGTCCCGATCCATGACGCCGGGTCGCGGCCGCTCCGATTCAATGCAGTACGGTTCGGGGGGTAACAGTGCTCGCTCGCCGGTCGGGTTCGCGTCCGAGGAGCTTTTGGCGGAGATGCTCGAGGCGCCCAAGGGTGGGGATAGCATATCGGTGACGATTCGGTTTCGGCCGCTGAGCGAGAGGGAGTTTCAGAGAGGGGATGAGGTCACTTGGTACGCAGACGGTGATAAGATTGTGAGGAATGAGTATAATCCAGCTACAGCCTATGCATTTGATAGAGTTTTTGGACAGCATGCCAATTCACAAGAGGTGTACGAAGTTGCCGCTAAACCTGTTGTCAAAGCTGCCATGGAAGGTGTCAATGGTACTGTTTTTGCTTATGGTGTGACAAGTAGTGGAAAGACACACACTATGCATGGTGATCAAAACGCTCCAGGTGTCATACCATTGGCGATAAAGGATGTCTTCAGTATTATTCAAGATACCCCAGGAAGGGAATTTTTACTGCGCGTCTCATACCTTGAGATCTACAATGAGGTGATAAATGACTTGCTTGATCCAACAGGTCAAAATTTGCGTGTTAGAGAAGATTCCCAGGGTACTTATGTTGAGGGTATAAAAGAAGAAGTTGTTTTGTCTCCTGGGCATGCACTTTCTTTTATTGCTGCTGGAGAAGAGCATCGTCATGTTGGATCAAACAATTTTAATCTGTTCAGCAGTCGAAGTCACACCATATTTACATTGATGATAGAAAGCAGTGTCCATGGTGATGAGTATGATGGAGTGATCTTCTCTCAACTTAATTTAATTGATCTAGCTGGATCTGAGAGCTCGAAAACCGAAACAACTGGACTAAGAAGAAAGGAAGGATCATACATAAACAAGAGTCTTCTAACTCTTGGAACTGTAATCGGAAAGCTGAGTGAGGGAAAGGCATCCCATGTACCATATCGAGATTCTAAGCTTACCCGTCTTTTACAATCTTCGTTAAGCGGGCATGGACATGTTTCGCTTATTTGCACGGTGACTCCTGCATCAAGTAGCATGGAGGAAACTCATAATACATTGAAATTTGCAAGCAGGGCAAAGCGAGTAGAAATCTATGCATCGCGTAATAAGATTATTGATGAAAAGTCATTGATTAAGAAGTATCAAAGAGAGATTTCGGTCCTGAAAACAGAACTTGATCAGCTAAGGCAGGGGATGCTTGTTGGCATCAGTCATGAGGAGATTATCAGCTTAAAGCAAAAGTTGGAAGAAGGTCAATTTAAAATGCAATCAAGATTGGAGGAAGAAGAGGAAGCGAAGGCTGCTCTTATGAGTAGAATCCAGAGGCTGACGAAGCTCATCCTTGTTTCTTCGAAAAATACAATCCCTGGATTGAGTGATATTCCCAGCCACCAACGCGGTTTTTCTGTTGGTGAGGATGACAAAATGGAAGTAGTGCGAGATGGACCCTTGCTTCTAGAAGGTGAGAACCAAAAGGAGTCACCATCTTCTGCATCTACTGTTCCATCAGATATGGCTTCTGATTTTAGACACAAGAGATCTTCCAGCAGGTGGAATGAAGAGCTCTCACCAGCTGGCGGTACAATTACAGATTCAACTCAAGCGGGTGAACTTATCAGTGGTTCAAGAATTCCAATGGGAGGGATGACAATGTCAGATCACATAGACCTGCTGGTTGAGCAAGTTAAGATGCTTGCTGGAGAGATTGCACTGGGCACCAGCTCCTTGAAACGACTGGTGGAGCAGTCTGTAGATGACCCTGAAAGCTCCAAAACTCAAATTGAGAACTTGGAATGTGATATCCATGAAAAGAGAAGGCAAATGAGGGTTTTGGAACAGCGCATTAACGAAAGTGGTGAGGCTTCAATTGCTAATGCATCTATGGTTGAGATGCAGCAGACAGTTAAGAGACTGACAACCCAGTGCAATGAGAAGGGATTTGAGTTGGAAATAAAATCAGCAGACAATCGTATTCTCCAGGAGCAATTGCAAAATAAGTGTGCAGAGAACATGGAATTGCAAGAAAAAGTGGATCAGTTAGAGCAGCGTTTGGCTTCAGTATCTGGTGAAGGATCATCATCGTCTTCCGAGCAGTGTGTATCTGCAGAATATGTTGAGCAGTTGCAAAAGAAAATTCAGTCTCAGGAGATTGAGAATGAAAAACTAAAGCTGGAGCATGTGCAGTTCTCAGAGGAGAGGAGTGGGTTACATGTGCAGAATCAAAAACTGGCCGAAGAAGCTTCTTATGCAAAGGAACTGGCATCTGCTGCTGCTGTTGAATTGAAGAATTTGGCTGGTGAAGTGACAAAGCTCTCATTGCAGAATGCAAAACTAGAAAAAGAGTTGTTGGCTGCCCGGGAGTTGGTCAATTCTAAAAGTTCTTCCATGCAACCTGTTAATGGTATTAATCGGAAGTACAATGATAGACCAAGAGGTGGGAGGAAAGGGAGGCTATCTGGTGGTGCTGATAACTTTGAGGCATGGAATCTTGATTCGGATGATTTAAGGATGGAACTCCAAGCAAGGAAACAGCGAGAGGCAGCTCTTGAGACTGCCCTTGCTGAAAAGGAATTTACTGAAGAGGAGTACAGGAAAAAGGTTGAAGATGCAAAGAAAAGGGAGGACGCTCTAGAAAATGATTTAGCAAACATGTGGGTGTTGGTCGCAAAATTAAAGAAAGAGGGCGGGTCTATCCCTGAGACACACACAGAAGAAAGGCATGATGATGTGATAGAAAATAGTAATGGTCTAAAAGCAACTGTGAATGAGGGTGCCGCCGTAGAGAGACAAGTTTTGGATGCTTCGAAATCCGCTGATGATGAAAGTCCTAAGGAAGAACCTCTTGTTCTTCGCCTTAAGGCTCGAATGCAAGAGATGAAGGAGAAAGAGCTCAAACACCAGGGAAACGGAGATGCCAATTCCCATTTGTGCAAAGTATGTTTTGAATCACCAACAGCAGCAATTCTTCTCCCTTGCCGGCATTTTTGTTTGTGTAAGTCTTGTTCACTTGCATGTTCTGAGTGCCCAATTTGTCGTACAAAGATTTCAGATAGGCTTTTTGCATTTACTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031347 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0065007 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin zf-C3HC4_3

Protein Analysis

1083

Amino Acids

119.5

Weight (kDa)

5.69

Isoelectric Point (pI)

51.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	109 - 420	3.9e-105	Kinesin motor domain
Microtub_bd	PF16796	127 - 240	7e-20	Microtubule binding
zf-C3HC4_3	PF13920	1032 - 1075	1.3e-11	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21380 AT2G21380 AT4G39050
fragaria_vesca	FvH4_2g10080
malus_domestica	MD05G1058100.v1.1 MD10G1065900.v1.1
prunus_persica	Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1
pyrus_communis	pycom05g04920 pycom10g05130 pycom10g05140
rosa_chinensis	RchiOBHm_Chr6g0267011
rosa_laevigata	RLG00000014051 RLG00000027737 RLG00000037008
rosa_multiflora	Rmu_co8233357.1_g000001 Rmu_sc0013804.1_g000010 Rmu_sc0017071.1_g000008 Rmu_sc0037796.1_g000001 Rmu_sc0041080.1_g000001 Rmu_ssc0000341.1_g000028
rosa_roxburghii	Rroxscaffold_2G00112220 Rroxscaffold_7G00201230
rosa_rugosa	Rorug06G0028400 Rorug06G0028400 Rorug06G0028400
rosa_samantha	Rh1AG191400 Rh1CG176500 Rh6AG150000 Rh6CG146100
rosa_wichuraiana	Rw6G012990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1713
Acc36I	ACCTGC	2 cut(s)	1713, 1851
AccB1I	GGYRCC	3 cut(s)	289, 1889, 2945
AccB7I	CCANNNNNTGG	2 cut(s)	1725, 2609
AccBSI	CCGCTC	1 cut(s)	198
AccII	CGCG	7 cut(s)	89, 115, 192, 257, 675, 1276, 1572
AclWI	GGATC	7 cut(s)	168, 716, 865, 994, 1042, 2214, 2254
AcoI	YGGCCR	3 cut(s)	193, 332, 2403
AcsI	RAATTY	7 cut(s)	662, 736, 1235, 1803, 2307, 2461, 2744
AcuI	CTGAAG	3 cut(s)	616, 2214, 2772
AcvI	CACGTG	1 cut(s)	17
AcyI	GRCGYC	2 cut(s)	182, 290
AflII	CTTAAG	1 cut(s)	3029
AflIII	ACRYGT	3 cut(s)	1168, 2383, 2823
AhdI	GACNNNNNGTC	1 cut(s)	186
AjnI	CCWGG	7 cut(s)	598, 652, 759, 805, 1540, 2151, 3072
Alw21I	GWGCWC	4 cut(s)	238, 998, 1740, 3066
Alw26I	GTCTC	7 cut(s)	682, 807, 2077, 2319, 2717, 2867, 2953
AlwI	GGATC	7 cut(s)	168, 716, 865, 994, 1042, 2214, 2254
AlwNI	CAGNNNCTG	2 cut(s)	1501, 2237
Ama87I	CYCGRG	4 cut(s)	151, 164, 284, 2529
AoxI	GGCC	5 cut(s)	3, 132, 193, 332, 2403
ApoI	RAATTY	7 cut(s)	662, 736, 1235, 1803, 2307, 2461, 2744
AseI	ATTAAT	1 cut(s)	2580
AspLEI	GCGC	4 cut(s)	117, 292, 675, 2016
AspS9I	GGNCC	3 cut(s)	168, 1333, 1619
AsuC2I	CCSGG	3 cut(s)	186, 2530, 2531
AsuII	TTCGAA	2 cut(s)	1526, 2978
AvaI	CYCGRG	4 cut(s)	151, 164, 284, 2529
AvaII	GGWCC	3 cut(s)	168, 1333, 1619
AxyI	CCTNAGG	1 cut(s)	3003
BaeGI	GKGCMC	3 cut(s)	22, 1892, 3200
BaeI	ACNNNNGTAYC	2 cut(s)	756, 789
BanI	GGYRCC	3 cut(s)	289, 1889, 2945
BanII	GRGCYC	3 cut(s)	998, 1740, 3066
BbrPI	CACGTG	1 cut(s)	17
BbsI	GAAGAC	4 cut(s)	75, 622, 1046, 2250
Bbv12I	GWGCWC	4 cut(s)	238, 998, 1740, 3066
BccI	CCATC	6 cut(s)	61, 938, 1610, 1666, 1687, 2677
BceAI	ACGGC	1 cut(s)	2936
BcgI	CGANNNNNNTGC	1 cut(s)	3225
BciT130I	CCWGG	7 cut(s)	600, 654, 761, 807, 1542, 2153, 3074
BclI	TGATCA	3 cut(s)	93, 586, 1351
BcnI	CCSGG	3 cut(s)	186, 2530, 2531
BcoDI	GTCTC	7 cut(s)	682, 807, 2077, 2319, 2717, 2867, 2953
BfaI	CTAG	4 cut(s)	980, 1631, 2507, 2804
BfmI	CTRYAG	3 cut(s)	423, 1923, 2277
BfoI	RGCGCY	1 cut(s)	293
BfrI	CTTAAG	1 cut(s)	3029
BfuAI	ACCTGC	2 cut(s)	1713, 1851
BglII	AGATCT	2 cut(s)	690, 1712
BlpI	GCTNAGC	1 cut(s)	338
Bme18I	GGWCC	3 cut(s)	168, 1333, 1619
BmeRI	GACNNNNNGTC	1 cut(s)	186
BmeT110I	CYCGRG	4 cut(s)	151, 164, 284, 2529
BmgT120I	GGNCC	3 cut(s)	168, 1333, 1619
BmiI	GGNNCC	6 cut(s)	169, 170, 291, 1621, 1891, 2947
BmrI	ACTGGG	2 cut(s)	1895, 2089
BmuI	ACTGGG	2 cut(s)	1895, 2089
BoxI	GACNNNNGTC	1 cut(s)	166
BpiI	GAAGAC	4 cut(s)	75, 622, 1046, 2250
BplI	GAGNNNNNCTC	2 cut(s)	147, 179
BpmI	CTGGAG	5 cut(s)	582, 855, 1893, 2135, 2366
Bpu10I	CCTNAGC	1 cut(s)	1358
Bpu1102I	GCTNAGC	1 cut(s)	338
Bpu14I	TTCGAA	2 cut(s)	1526, 2978
BpuEI	CTTGAG	3 cut(s)	707, 1758, 2741
BpuMI	CCSGG	3 cut(s)	186, 2530, 2531
BsaAI	YACGTR	1 cut(s)	17
BsaBI	GATNNNNATC	1 cut(s)	2251
BsaHI	GRCGYC	2 cut(s)	182, 290
BsaWI	WCCGGW	1 cut(s)	45
BsaXI	ACNNNNNCTCC	2 cut(s)	1177, 1207
Bse118I	RCCGGY	2 cut(s)	244, 3150
Bse1I	ACTGG	7 cut(s)	57, 1018, 1890, 1914, 2095, 2406, 2436
Bse21I	CCTNAGG	1 cut(s)	3003
Bse3DI	GCAATG	2 cut(s)	2107, 2489
Bse8I	GATNNNNATC	1 cut(s)	2251
BseBI	CCWGG	7 cut(s)	600, 654, 761, 807, 1542, 2153, 3074
BseJI	GATNNNNATC	1 cut(s)	2251
BseMI	GCAATG	2 cut(s)	2107, 2489
BseMII	CTCAG	7 cut(s)	329, 981, 1076, 2330, 2376, 2862, 3183
BseNI	ACTGG	7 cut(s)	57, 1018, 1890, 1914, 2095, 2406, 2436
BseRI	GAGGAG	8 cut(s)	58, 67, 127, 278, 1406, 2381, 2386, 2771
BseSI	GKGCMC	3 cut(s)	22, 1892, 3200
BseX3I	CGGCCG	2 cut(s)	193, 332
BseYI	CCCAGC	1 cut(s)	1559
BsgI	GTGCAG	3 cut(s)	2196, 2375, 2408
Bsh1236I	CGCG	7 cut(s)	89, 115, 192, 257, 675, 1276, 1572
Bsh1285I	CGRYCG	4 cut(s)	103, 196, 249, 335
BshFI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BshNI	GGYRCC	3 cut(s)	289, 1889, 2945
BsiEI	CGRYCG	4 cut(s)	103, 196, 249, 335
BsiHKAI	GWGCWC	4 cut(s)	238, 998, 1740, 3066
BsiHKCI	CYCGRG	4 cut(s)	151, 164, 284, 2529
BsiSI	CCGG	5 cut(s)	46, 185, 245, 2530, 3151
BslFI	GGGAC	2 cut(s)	45, 154
BsmAI	GTCTC	7 cut(s)	682, 807, 2077, 2319, 2717, 2867, 2953
BsmBI	CGTCTC	1 cut(s)	682
BsmFI	GGGAC	2 cut(s)	45, 154
BsmI	GAATGC	2 cut(s)	2503, 3045
BsnI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BsoBI	CYCGRG	4 cut(s)	151, 164, 284, 2529
Bsp119I	TTCGAA	2 cut(s)	1526, 2978
Bsp1286I	GDGCHC	7 cut(s)	22, 238, 998, 1740, 1892, 3066, 3200
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	2 cut(s)	513, 928
BspANI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BspCNI	CTCAG	7 cut(s)	330, 982, 1077, 2329, 2375, 2863, 3184
BspFNI	CGCG	7 cut(s)	89, 115, 192, 257, 675, 1276, 1572
BspHI	TCATGA	1 cut(s)	1387
BspLI	GGNNCC	6 cut(s)	169, 170, 291, 1621, 1891, 2947
BspMAI	CTGCAG	1 cut(s)	2281
BspMI	ACCTGC	2 cut(s)	1713, 1851
BspPI	GGATC	7 cut(s)	168, 716, 865, 994, 1042, 2214, 2254
BspQI	GCTCTTC	2 cut(s)	834, 1728
BspT104I	TTCGAA	2 cut(s)	1526, 2978
BspT107I	GGYRCC	3 cut(s)	289, 1889, 2945
BspTI	CTTAAG	1 cut(s)	3029
BsrBI	CCGCTC	1 cut(s)	198
BsrDI	GCAATG	2 cut(s)	2107, 2489
BsrFI	RCCGGY	2 cut(s)	244, 3150
BsrI	ACTGG	7 cut(s)	57, 1018, 1890, 1914, 2095, 2406, 2436
BssAI	RCCGGY	2 cut(s)	244, 3150
BssNI	GRCGYC	2 cut(s)	182, 290
BssT1I	CCWWGG	3 cut(s)	294, 513, 928
Bst2UI	CCWGG	7 cut(s)	600, 654, 761, 807, 1542, 2153, 3074
Bst6I	CTCTTC	4 cut(s)	834, 1455, 1728, 2748
BstACI	GRCGYC	2 cut(s)	182, 290
BstAFI	CTTAAG	1 cut(s)	3029
BstAPI	GCANNNNNTGC	2 cut(s)	1369, 2441
BstBAI	YACGTR	1 cut(s)	17
BstBI	TTCGAA	2 cut(s)	1526, 2978
BstDSI	CCRYGG	2 cut(s)	513, 928
BstFNI	CGCG	7 cut(s)	89, 115, 192, 257, 675, 1276, 1572
BstH2I	RGCGCY	1 cut(s)	293
BstHHI	GCGC	4 cut(s)	117, 292, 675, 2016
BstMAI	GTCTC	7 cut(s)	682, 807, 2077, 2319, 2717, 2867, 2953
BstMCI	CGRYCG	4 cut(s)	103, 196, 249, 335
BstNI	CCWGG	7 cut(s)	600, 654, 761, 807, 1542, 2153, 3074
BstNSI	RCATGY	7 cut(s)	461, 815, 1172, 2354, 2387, 2827, 3189
BstPAI	GACNNNNGTC	1 cut(s)	166
BstSFI	CTRYAG	3 cut(s)	423, 1923, 2277
BstSLI	GKGCMC	3 cut(s)	22, 1892, 3200
BstUI	CGCG	7 cut(s)	89, 115, 192, 257, 675, 1276, 1572
BstV2I	GAAGAC	4 cut(s)	75, 622, 1046, 2250
BstX2I	RGATCY	3 cut(s)	690, 986, 1712
BstYI	RGATCY	3 cut(s)	690, 986, 1712
BstZI	CGGCCG	2 cut(s)	193, 332
Bsu36I	CCTNAGG	1 cut(s)	3003
BsuRI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BtgI	CCRYGG	2 cut(s)	513, 928
BtgZI	GCGATG	1 cut(s)	1257
BtsI	GCAGTG	2 cut(s)	928, 2273
BtsIMutI	CAGTG	6 cut(s)	238, 928, 1798, 1883, 2102, 2273
BveI	ACCTGC	2 cut(s)	1713, 1851
CaiI	CAGNNNCTG	2 cut(s)	1501, 2237
CciI	TCATGA	1 cut(s)	1387
CciNI	GCGGCCGC	1 cut(s)	193
CfoI	GCGC	4 cut(s)	117, 292, 675, 2016
Cfr10I	RCCGGY	2 cut(s)	244, 3150
Cfr13I	GGNCC	3 cut(s)	168, 1333, 1619
Cfr9I	CCCGGG	1 cut(s)	2529
CseI	GACGC	4 cut(s)	190, 246, 664, 2807
DinI	GGCGCC	1 cut(s)	291
DraI	TTTAAA	1 cut(s)	1435
DriI	GACNNNNNGTC	1 cut(s)	186
EaeI	YGGCCR	3 cut(s)	193, 332, 2403
EagI	CGGCCG	2 cut(s)	193, 332
Eam1104I	CTCTTC	4 cut(s)	834, 1455, 1728, 2748
Eam1105I	GACNNNNNGTC	1 cut(s)	186
EarI	CTCTTC	4 cut(s)	834, 1455, 1728, 2748
EciI	GGCGGA	1 cut(s)	290
Ecl136II	GAGCTC	3 cut(s)	996, 1738, 3064
EclXI	CGGCCG	2 cut(s)	193, 332
Eco130I	CCWWGG	3 cut(s)	294, 513, 928
Eco24I	GRGCYC	3 cut(s)	998, 1740, 3066
Eco32I	GATATC	1 cut(s)	1975
Eco47I	GGWCC	3 cut(s)	168, 1333, 1619
Eco52I	CGGCCG	2 cut(s)	193, 332
Eco53kI	GAGCTC	3 cut(s)	996, 1738, 3064
Eco57I	CTGAAG	3 cut(s)	616, 2214, 2772
Eco72I	CACGTG	1 cut(s)	17
Eco81I	CCTNAGG	1 cut(s)	3003
Eco88I	CYCGRG	4 cut(s)	151, 164, 284, 2529
EcoICRI	GAGCTC	3 cut(s)	996, 1738, 3064
EcoO109I	RGGNCCY	1 cut(s)	168
EcoRI	GAATTC	1 cut(s)	1803
EcoRII	CCWGG	7 cut(s)	598, 652, 759, 805, 1540, 2151, 3072
EcoRV	GATATC	1 cut(s)	1975
EcoT14I	CCWWGG	3 cut(s)	294, 513, 928
EcoT22I	ATGCAT	4 cut(s)	436, 582, 1273, 2053
EcoT38I	GRGCYC	3 cut(s)	998, 1740, 3066
EgeI	GGCGCC	1 cut(s)	291
EheI	GGCGCC	1 cut(s)	291
ErhI	CCWWGG	3 cut(s)	294, 513, 928
Esp3I	CGTCTC	1 cut(s)	682
FalI	AAGNNNNNCTT	4 cut(s)	614, 646, 3000, 3032
FaqI	GGGAC	2 cut(s)	45, 154
FauI	CCCGC	4 cut(s)	29, 1151, 1771, 2852
FbaI	TGATCA	3 cut(s)	93, 586, 1351
FriOI	GRGCYC	3 cut(s)	998, 1740, 3066
FspBI	CTAG	4 cut(s)	980, 1631, 2507, 2804
GlaI	GCGC	4 cut(s)	116, 291, 674, 2015
GsaI	CCCAGC	1 cut(s)	1563
GsuI	CTGGAG	5 cut(s)	582, 855, 1893, 2135, 2366
HaeII	RGCGCY	1 cut(s)	293
HaeIII	GGCC	5 cut(s)	5, 134, 195, 334, 2405
HapII	CCGG	5 cut(s)	46, 185, 245, 2530, 3151
HgaI	GACGC	4 cut(s)	190, 246, 664, 2807
HhaI	GCGC	4 cut(s)	117, 292, 675, 2016
Hin1I	GRCGYC	2 cut(s)	182, 290
Hin6I	GCGC	4 cut(s)	115, 290, 673, 2014
HinP1I	GCGC	4 cut(s)	115, 290, 673, 2014
HindIII	AAGCTT	2 cut(s)	1127, 2412
HpaII	CCGG	5 cut(s)	46, 185, 245, 2530, 3151
Hpy166II	GTNNAC	4 cut(s)	161, 478, 1784, 3179
Hpy8I	GTNNAC	4 cut(s)	161, 478, 1784, 3179
HpyCH4IV	ACGT	1 cut(s)	16
HpySE526I	ACGT	1 cut(s)	16
Hsp92I	GRCGYC	2 cut(s)	182, 290
HspAI	GCGC	4 cut(s)	115, 290, 673, 2014
KasI	GGCGCC	1 cut(s)	289
KflI	GGGWCCC	1 cut(s)	168
KroI	GCCGGC	1 cut(s)	3150
KroNI	GCCGGC	1 cut(s)	3152
Ksp22I	TGATCA	3 cut(s)	93, 586, 1351
LguI	GCTCTTC	2 cut(s)	834, 1728
LmnI	GCTCC	9 cut(s)	122, 203, 265, 601, 1901, 1915, 1946, 2155, 2347
MaeI	CTAG	4 cut(s)	980, 1631, 2507, 2804
MaeII	ACGT	1 cut(s)	16
MaeIII	GTNAC	9 cut(s)	227, 316, 370, 550, 890, 1189, 1653, 2379, 2476
MbiI	CCGCTC	1 cut(s)	198
MfeI	CAATTG	2 cut(s)	2040, 2159
MflI	RGATCY	3 cut(s)	690, 986, 1712
MhlI	GDGCHC	7 cut(s)	22, 238, 998, 1740, 1892, 3066, 3200
Mly113I	GGCGCC	1 cut(s)	290
MlyI	GAGTC	4 cut(s)	156, 1060, 1186, 1661
MmeI	TCCRAC	5 cut(s)	83, 749, 835, 1398, 2100
Mph1103I	ATGCAT	4 cut(s)	436, 582, 1273, 2053
MroNI	GCCGGC	1 cut(s)	3150
MslI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2828, 2898, 3184
MspA1I	CMGCKG	3 cut(s)	337, 1748, 2987
MspCI	CTTAAG	1 cut(s)	3029
MspI	CCGG	5 cut(s)	46, 185, 245, 2530, 3151
MunI	CAATTG	2 cut(s)	2040, 2159
Mva1269I	GAATGC	2 cut(s)	2503, 3045
MvaI	CCWGG	7 cut(s)	600, 654, 761, 807, 1542, 2153, 3074
MvnI	CGCG	7 cut(s)	89, 115, 192, 257, 675, 1276, 1572
NaeI	GCCGGC	1 cut(s)	3152
NarI	GGCGCC	1 cut(s)	290
NciI	CCSGG	3 cut(s)	186, 2530, 2531
NcoI	CCATGG	2 cut(s)	513, 928
NgoMIV	GCCGGC	1 cut(s)	3150
NlaIV	GGNNCC	6 cut(s)	169, 170, 291, 1621, 1891, 2947
NmeAIII	GCCGAG	1 cut(s)	110
NmuCI	GTSAC	7 cut(s)	316, 370, 550, 890, 1189, 1653, 2476
NotI	GCGGCCGC	1 cut(s)	193
NsiI	ATGCAT	4 cut(s)	436, 582, 1273, 2053
NspI	RCATGY	7 cut(s)	461, 815, 1172, 2354, 2387, 2827, 3189
NspV	TTCGAA	2 cut(s)	1526, 2978
PaeI	GCATGC	2 cut(s)	461, 815
PaeR7I	CTCGAG	1 cut(s)	284
PagI	TCATGA	1 cut(s)	1387
PaqCI	CACCTGC	1 cut(s)	1713
PasI	CCCWGGG	1 cut(s)	760
PciI	ACATGT	3 cut(s)	1168, 2383, 2823
PciSI	GCTCTTC	2 cut(s)	834, 1728
PcsI	WCGNNNNNNNCGW	2 cut(s)	86, 137
PctI	GAATGC	2 cut(s)	2503, 3045
PdiI	GCCGGC	1 cut(s)	3152
PflFI	GACNNNGTC	1 cut(s)	1825
PflMI	CCANNNNNTGG	2 cut(s)	1725, 2609
PfoI	TCCNGGA	1 cut(s)	2151
PleI	GAGTC	4 cut(s)	156, 1059, 1186, 1660
PluTI	GGCGCC	1 cut(s)	293
PmaCI	CACGTG	1 cut(s)	17
PmlI	CACGTG	1 cut(s)	17
PpsI	GAGTC	4 cut(s)	156, 1059, 1186, 1660
Ppu21I	YACGTR	1 cut(s)	17
PpuMI	RGGWCCY	1 cut(s)	168
PscI	ACATGT	3 cut(s)	1168, 2383, 2823
PshAI	GACNNNNGTC	1 cut(s)	166
PshBI	ATTAAT	1 cut(s)	2580
Psp124BI	GAGCTC	3 cut(s)	998, 1740, 3066
Psp5II	RGGWCCY	1 cut(s)	168
Psp6I	CCWGG	7 cut(s)	598, 652, 759, 805, 1540, 2151, 3072
PspCI	CACGTG	1 cut(s)	17
PspFI	CCCAGC	1 cut(s)	1559
PspGI	CCWGG	7 cut(s)	598, 652, 759, 805, 1540, 2151, 3072
PspN4I	GGNNCC	6 cut(s)	169, 170, 291, 1621, 1891, 2947
PspPI	GGNCC	3 cut(s)	168, 1333, 1619
PspPPI	RGGWCCY	1 cut(s)	168
PspXI	VCTCGAGB	1 cut(s)	284
PsrI	GAACNNNNNNTAC	2 cut(s)	3160, 3192
PstI	CTGCAG	1 cut(s)	2281
PstNI	CAGNNNCTG	2 cut(s)	1501, 2237
PsuI	RGATCY	3 cut(s)	690, 986, 1712
PsyI	GACNNNGTC	1 cut(s)	1825
PvuII	CAGCTG	1 cut(s)	1748
RseI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2828, 2898, 3184
SacI	GAGCTC	3 cut(s)	998, 1740, 3066
SapI	GCTCTTC	2 cut(s)	834, 1728
Sau96I	GGNCC	3 cut(s)	168, 1333, 1619
SchI	GAGTC	4 cut(s)	156, 1060, 1186, 1661
SduI	GDGCHC	7 cut(s)	22, 238, 998, 1740, 1892, 3066, 3200
SfcI	CTRYAG	3 cut(s)	423, 1923, 2277
SfoI	GGCGCC	1 cut(s)	291
Sfr274I	CTCGAG	1 cut(s)	284
SfuI	TTCGAA	2 cut(s)	1526, 2978
SinI	GGWCC	3 cut(s)	168, 1333, 1619
SlaI	CTCGAG	1 cut(s)	284
SmaI	CCCGGG	1 cut(s)	2531
SmiMI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2828, 2898, 3184
SmlI	CTYRAG	5 cut(s)	284, 686, 1773, 2720, 3029
SmoI	CTYRAG	5 cut(s)	284, 686, 1773, 2720, 3029
SphI	GCATGC	2 cut(s)	461, 815
SspDI	GGCGCC	1 cut(s)	289
SspMI	CTAG	4 cut(s)	980, 1631, 2507, 2804
SstI	GAGCTC	3 cut(s)	998, 1740, 3066
StyI	CCWWGG	3 cut(s)	294, 513, 928
TaiI	ACGT	1 cut(s)	19
TaqI	TCGA	8 cut(s)	125, 285, 886, 998, 1117, 1526, 2978, 3037
TaqII	GACCGA	1 cut(s)	1321
TatI	WGTACW	2 cut(s)	2589, 2760
TauI	GCSGC	5 cut(s)	195, 198, 337, 491, 2951
TscAI	CASTG	6 cut(s)	238, 928, 1798, 1890, 2102, 2273
TseFI	GTSAC	7 cut(s)	316, 370, 550, 890, 1189, 1653, 2476
Tsp45I	GTSAC	7 cut(s)	316, 370, 550, 890, 1189, 1653, 2476
TspDTI	ATGAA	6 cut(s)	1308, 1746, 1995, 2346, 3009, 3065
TspGWI	ACGGA	1 cut(s)	3096
TspMI	CCCGGG	1 cut(s)	2529
TspRI	CASTG	6 cut(s)	238, 928, 1798, 1890, 2102, 2273
Tth111I	GACNNNGTC	1 cut(s)	1825
Van91I	CCANNNNNTGG	2 cut(s)	1725, 2609
Vha464I	CTTAAG	1 cut(s)	3029
VpaK11BI	GGWCC	3 cut(s)	168, 1333, 1619
VspI	ATTAAT	1 cut(s)	2580
XapI	RAATTY	7 cut(s)	662, 736, 1235, 1803, 2307, 2461, 2744
XbaI	TCTAGA	2 cut(s)	1630, 2803
XceI	RCATGY	7 cut(s)	461, 815, 1172, 2354, 2387, 2827, 3189
XhoI	CTCGAG	1 cut(s)	284
XmaI	CCCGGG	1 cut(s)	2529
XspI	CTAG	4 cut(s)	980, 1631, 2507, 2804
Zsp2I	ATGCAT	4 cut(s)	436, 582, 1273, 2053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.