Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004310

Physical Location & Seq

Genomic Coordinates Forward (+)

9017185 .. 9028786

Gene Structure 11602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1065900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGGCCTCGTCCTCACGAGCCCGCAGCAGCTCTCCTTTCTCCTACCGGAAGCCATCCAGTCCCTACTCCTCGACCTCTTCATCTTCTTCGTTGATCAACGGTCGGATGATTCCGCGCTCCTGCTCGACCTCGGCCTCGTCGTTCTACAACTCTGGAGGTGGGCTCGGGTCCCGATCCGTGACGCCGAGCCACGGCCGCTCCGATTCAATGCAGTACGGGTCGGGGGGTTACAGTGCTCGCTCGCCCGTCGGGTTCGCGTCCGAGGAGCTTTTGGCGGAGATGCTCGAGGCGCCCAAGGGTGGGGATAGCATATCGGTGACGATTCGGTTTCGGCCGCTGAGCGAGAGGGAGTTTCAGAGAGGGGATGAGGTCACTTGGTACGCAGACGGCGATAAGATTGTGAGGAATGAGTATAATCCAGCTACAGCCTATGCATTTGATAGAGTTTTTGGACAGCATGCCAATTCGCAAGAGGTGTACGAAGTTGCTGCTAAACCTGTTGTCAAGGCTGCCATGGAAGGTGTCAATGGTACTGTTTTTGCTTATGGTGTGACAAGTAGTGGAAAGACACACACTATGCATGGTGATCAAAACGCTCCAGGTATCATACCATTGGCGATAAAGGATGTCTTCAGTATTATCCAAGATACCCCAGGAAGGGAATTTTTACTCCGCGTCTCATATCTTGAGATCTACAACGAGGTGATAAATGACTTGCTTGATCCAACAGGTCAAAATTTGCGTGTTAGAGAAGATTCACAGGGTACTTATGTTGAGGGTATAAAAGAAGAAGTTGTTTTGTCTCCTGGGCATGCACTTTCTTTTATTGCTGCTGGAGAAGAGCATCGTCATGTTGGATCAAACAATTTTAATCTGTTGAGTAGTCGAAGTCACACCATATTTACACTGATGATTGAAAGCAGTGCCCATGGTGATGAGTATGACGGAGTGATCTTCTCTCAACTTAATTTAATTGATCTAGCTGGATCTGAGAGCTCGAAAACCGAAACAACTGGACTAAGAAGAAAGGAAGGATCATACATAAACAAGAGTCTTCTAACTCTTGGAACTGTAATTGGAAAGCTGAGTGAGGGAAAGGCATCCCATGTTCCATATCGAGATTCTAAGCTTACCCGCCTTTTACAATCCTCGTTAGGTGGGCATGGACATGTTTCGCTTATTTGCACGGTGACTCCTGCATCAAGTAGCATGGAGGAAACTCATAATACATTGAAGTTTGCAAACAGGGCAAAGCGAGTAGAAATCTATGCATCGCGTAATAAGATTATTGATGAAAAGTCATTGATTAAGAAGTATCAAAGAGAGATTTCGGTCCTGAAAACAGAACTTGATCAGCTAAGGAAGGGGATGCTTGTTGGCATAAGTCATGAGGAGATTATCAGCTTAAAGCAAAAGTTGGAAGAAGGTCAATTTAAAATGCAATCAAGATTGGAGGAAGAAGAGGAAGCGAAGGCTGCTCTTATGAGTAGAATCCAGAGGCTGACGAAGCTTATCCTTGTTTCTTCAAAAAATACAATTCCTGGATTAGGTGATATTCCCAGCCACCAACGCAGTTTTTCTGTTGGTGAGGATGACAAAATGGAAGTAGTGCGAGATGGACCCTTGCTTCTAGAAGGTGAGAACCAAAAGGAGTCACCATCATCTGCATCTGCTGGTCCATCAGATATGGCTTATGATTTTAGACACAAGAGATCTTCCAGCAGGAGGAATGAAGAGCTCTCCCCAGCTGGCAGTACAATTACAGATTCAACTCAAGCGGGTGAACTTATCAGTGGTTCAAGAGTTCCGATGGGAGGGATGACAATGTCAGATCACATAGACCTGCTGGTTGAGCAAGTTAAGATGCTTGCTGGAGAGATTGCACTTGGCACCAGCTCCTTGAAACGATTGGTGGAGCAGTCTGTAGATGACCCTGAAAGCTCAAAAACTCAAATTGAGAACTTGGAACGTGATATCCATGAAAAGAGAAGGCAAATGAGAATTTTGGAACAGCGCATTAACGAAAGTGGTGAGGCTTCAATTGCTAATGCATCTATGGTTGAGATGCAGCAGACAGTTAAGAGACTGACGACCCAGTGCAATGAGAAGGGATTTGAATTGGAAATAAAATCAGCAGACAATCGTATTCTCCAGGAGCAATTGCAAAATAAGTGTGCAGAGAACATGGAGTTGCAAGAAAAAGTGGATCAGTTAGAGCGGCGTTTGGCTTCAGTATCTGGTGAAAGATCATCACCGTCTTCCGAGCACTGTGCATCTGAAGAGTATGTTGAGGAGTTGCAAAAGAAAATTCAGTCTCAGGAGATTGAGAATGAAAAACTAAAGCTGGAGCACGTGCGTTTCTCGGAGGAGAGTAGTGGGTTACATGTGCAGAATCAAAAACTGGCCGAAGAAGCTTCTTATGCAAAGGAACTGGCATCGGCTGCTGCTGTTGAGTTGAAGAATTTGGCTGGTGAAGTGACAAAGCTCTCATTGCAGAATGCAAAACTAGAAAAAGAATTGTTGGCTGCTCGGGAGGTAGCCAATTCTAGAAGTTCTGCCATGCAACCCGTTAATGGCATTAACCGGAAGTACAATGATGGACCAAGAGGTGGGAGGAAAGGGAGGCTATCTGGTCGTTCTGATGACTTTGAGGCATGGAATCATGATTCGGATGATTTAAAGATGGAACTCCAAGCAAGGAAACAGCGAGAGGCAGCTCTTGAGGCTGCCCTTGCTGAAAAGGAATTTATAGAAGAGGAGTACAGGAAAAAGGTTGAAGATGCAAAGAAAAGGGAGGAAGCTCTAGAGAATGATTTAGCAAACATGTGGGTGTTGGTCGCCAAATTAAAGAAAGAGGGCGGGTCTATCCCTGAGACACACACAGAAGAAAGGCATAATGATGTGATGGAAAATAATAATGGTCTAAAAGCAACTGTGAATGAGAGTGCCCACATAGAGATACAAGTTTTGGATGCTCCAAAATCTGCTGATGATGAAAGTCGTATGGAAGAACCTCTTGTTCTTCGCCTTAAGGCTCGAATGAAAGAGATGAAGGAGAAGGAGCTCAAACACCAGGGAAACGGAGATGCCAATTCCCATTTGTGTAAAGTATGTTTTGAATCACCAACTGCAGCAATTCTTCTCCCTTGCCGGCATTTTTGCTTGTGTAAATCTTGTTCACTTGCATGTTCTGAGTGCCCAATTTGTCGTACAAAGATTTCAGATAGGCTTTTTGCATTTACTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031347 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0065007 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin zf-C3HC4_3

Protein Analysis

1083

Amino Acids

119.83

Weight (kDa)

5.86

Isoelectric Point (pI)

55.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	109 - 420	3.2e-106	Kinesin motor domain
Microtub_bd	PF16796	127 - 240	9.4e-20	Microtubule binding
zf-C3HC4_3	PF13920	1032 - 1075	1.3e-11	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21380 AT2G21380 AT4G39050
fragaria_vesca	FvH4_2g10080
malus_domestica	MD05G1058100.v1.1 MD10G1065900.v1.1
prunus_persica	Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1
pyrus_communis	pycom05g04920 pycom10g05130 pycom10g05140
rosa_chinensis	RchiOBHm_Chr6g0267011
rosa_laevigata	RLG00000014051 RLG00000027737 RLG00000037008
rosa_multiflora	Rmu_co8233357.1_g000001 Rmu_sc0013804.1_g000010 Rmu_sc0017071.1_g000008 Rmu_sc0037796.1_g000001 Rmu_sc0041080.1_g000001 Rmu_ssc0000341.1_g000028
rosa_roxburghii	Rroxscaffold_2G00112220 Rroxscaffold_7G00201230
rosa_rugosa	Rorug06G0028400 Rorug06G0028400 Rorug06G0028400
rosa_samantha	Rh1AG191400 Rh1CG176500 Rh6AG150000 Rh6CG146100
rosa_wichuraiana	Rw6G012990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1851
AccB1I	GGYRCC	2 cut(s)	289, 1889
AccB7I	CCANNNNNTGG	1 cut(s)	2609
AccBSI	CCGCTC	2 cut(s)	198, 2218
AccII	CGCG	4 cut(s)	115, 257, 675, 1276
AclWI	GGATC	6 cut(s)	168, 716, 865, 994, 1042, 2214
AcoI	YGGCCR	3 cut(s)	193, 332, 2403
AcsI	RAATTY	6 cut(s)	662, 736, 2001, 2307, 2461, 2744
AcuI	CTGAAG	3 cut(s)	616, 2214, 2298
AcvI	CACGTG	1 cut(s)	2353
AcyI	GRCGYC	2 cut(s)	182, 290
AfaI	GTAC	9 cut(s)	215, 380, 479, 532, 766, 1756, 2591, 2762, 3211
AflII	CTTAAG	1 cut(s)	3029
AflIII	ACRYGT	3 cut(s)	1168, 2383, 2823
AjnI	CCWGG	6 cut(s)	598, 652, 805, 1540, 2151, 3072
AloI	GAACNNNNNNTCC	2 cut(s)	1959, 1991
Alw21I	GWGCWC	6 cut(s)	238, 998, 1740, 2268, 2352, 3066
Alw26I	GTCTC	5 cut(s)	682, 807, 2077, 2319, 2867
AlwI	GGATC	6 cut(s)	168, 716, 865, 994, 1042, 2214
AlwNI	CAGNNNCTG	2 cut(s)	1501, 2237
Ama87I	CYCGRG	3 cut(s)	164, 284, 2529
AoxI	GGCC	5 cut(s)	3, 132, 193, 332, 2403
ApoI	RAATTY	6 cut(s)	662, 736, 2001, 2307, 2461, 2744
AspLEI	GCGC	3 cut(s)	117, 292, 2016
AspS9I	GGNCC	5 cut(s)	168, 1333, 1619, 1676, 2600
AvaI	CYCGRG	3 cut(s)	164, 284, 2529
AvaII	GGWCC	5 cut(s)	168, 1333, 1619, 1676, 2600
BaeGI	GKGCMC	3 cut(s)	928, 2950, 3200
BaeI	ACNNNNGTAYC	2 cut(s)	756, 789
BanI	GGYRCC	2 cut(s)	289, 1889
BanII	GRGCYC	5 cut(s)	22, 165, 998, 1740, 3066
BauI	CACGAG	1 cut(s)	15
BbrPI	CACGTG	1 cut(s)	2353
BbsI	GAAGAC	3 cut(s)	622, 1046, 2250
Bbv12I	GWGCWC	6 cut(s)	238, 998, 1740, 2268, 2352, 3066
BccI	CCATC	8 cut(s)	61, 1610, 1666, 1687, 1804, 2591, 2677, 2899
BceAI	ACGGC	2 cut(s)	208, 403
BcgI	CGANNNNNNTGC	5 cut(s)	470, 504, 2252, 2286, 3225
BciT130I	CCWGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
BclI	TGATCA	3 cut(s)	93, 586, 1351
BcoDI	GTCTC	5 cut(s)	682, 807, 2077, 2319, 2867
BfaI	CTAG	5 cut(s)	980, 1631, 2507, 2547, 2804
BfmI	CTRYAG	3 cut(s)	423, 1923, 3129
BfoI	RGCGCY	1 cut(s)	293
BfrI	CTTAAG	1 cut(s)	3029
BfuAI	ACCTGC	1 cut(s)	1851
BglII	AGATCT	2 cut(s)	690, 1712
BlpI	GCTNAGC	1 cut(s)	338
Bme1390I	CCNGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
Bme18I	GGWCC	5 cut(s)	168, 1333, 1619, 1676, 2600
BmeT110I	CYCGRG	3 cut(s)	164, 284, 2529
BmgT120I	GGNCC	5 cut(s)	168, 1333, 1619, 1676, 2600
BmiI	GGNNCC	5 cut(s)	169, 170, 291, 1621, 1891
BmrFI	CCNGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
BmrI	ACTGGG	1 cut(s)	2089
BmuI	ACTGGG	1 cut(s)	2089
BpiI	GAAGAC	3 cut(s)	622, 1046, 2250
BplI	GAGNNNNNCTC	2 cut(s)	147, 179
BpmI	CTGGAG	6 cut(s)	174, 582, 855, 1893, 2135, 2366
Bpu10I	CCTNAGC	1 cut(s)	1358
Bpu1102I	GCTNAGC	1 cut(s)	338
BpuEI	CTTGAG	3 cut(s)	707, 1758, 2741
BsaAI	YACGTR	1 cut(s)	2353
BsaHI	GRCGYC	2 cut(s)	182, 290
BsaJI	CCNNGG	9 cut(s)	129, 190, 261, 294, 513, 652, 806, 928, 3073
BsaWI	WCCGGW	2 cut(s)	45, 2583
BsaXI	ACNNNNNCTCC	4 cut(s)	182, 212, 1177, 1207
Bse118I	RCCGGY	1 cut(s)	3150
Bse1I	ACTGG	5 cut(s)	57, 1018, 2095, 2406, 2436
Bse3DI	GCAATG	2 cut(s)	2107, 2489
BseBI	CCWGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
BseDI	CCNNGG	9 cut(s)	129, 190, 261, 294, 513, 652, 806, 928, 3073
BseMI	GCAATG	2 cut(s)	2107, 2489
BseMII	CTCAG	6 cut(s)	329, 981, 1076, 2330, 2862, 3183
BseNI	ACTGG	5 cut(s)	57, 1018, 2095, 2406, 2436
BseRI	GAGGAG	6 cut(s)	58, 278, 1406, 2306, 2381, 2771
BseSI	GKGCMC	3 cut(s)	928, 2950, 3200
BseX3I	CGGCCG	2 cut(s)	193, 332
BseYI	CCCAGC	2 cut(s)	1559, 1744
BsgI	GTGCAG	2 cut(s)	2196, 2408
Bsh1236I	CGCG	4 cut(s)	115, 257, 675, 1276
Bsh1285I	CGRYCG	3 cut(s)	103, 196, 335
BshFI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BshNI	GGYRCC	2 cut(s)	289, 1889
BsiEI	CGRYCG	3 cut(s)	103, 196, 335
BsiHKAI	GWGCWC	6 cut(s)	238, 998, 1740, 2268, 2352, 3066
BsiHKCI	CYCGRG	3 cut(s)	164, 284, 2529
BsiSI	CCGG	3 cut(s)	46, 2584, 3151
BslFI	GGGAC	2 cut(s)	45, 154
BsmAI	GTCTC	5 cut(s)	682, 807, 2077, 2319, 2867
BsmBI	CGTCTC	1 cut(s)	682
BsmFI	GGGAC	2 cut(s)	45, 154
BsmI	GAATGC	1 cut(s)	2503
BsnI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BsoBI	CYCGRG	3 cut(s)	164, 284, 2529
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	2 cut(s)	513, 928
BspANI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BspCNI	CTCAG	6 cut(s)	330, 982, 1077, 2329, 2863, 3184
BspFNI	CGCG	4 cut(s)	115, 257, 675, 1276
BspHI	TCATGA	2 cut(s)	1387, 2662
BspLI	GGNNCC	5 cut(s)	169, 170, 291, 1621, 1891
BspMAI	CTGCAG	1 cut(s)	3133
BspMI	ACCTGC	1 cut(s)	1851
BspPI	GGATC	6 cut(s)	168, 716, 865, 994, 1042, 2214
BspQI	GCTCTTC	2 cut(s)	834, 1728
BspT107I	GGYRCC	2 cut(s)	289, 1889
BspTI	CTTAAG	1 cut(s)	3029
BsrBI	CCGCTC	2 cut(s)	198, 2218
BsrDI	GCAATG	2 cut(s)	2107, 2489
BsrFI	RCCGGY	1 cut(s)	3150
BsrI	ACTGG	5 cut(s)	57, 1018, 2095, 2406, 2436
BssAI	RCCGGY	1 cut(s)	3150
BssECI	CCNNGG	9 cut(s)	129, 190, 261, 294, 513, 652, 806, 928, 3073
BssNI	GRCGYC	2 cut(s)	182, 290
BssSI	CACGAG	1 cut(s)	15
BssT1I	CCWWGG	3 cut(s)	294, 513, 928
Bst2BI	CACGAG	1 cut(s)	15
Bst2UI	CCWGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
Bst4CI	ACNGT	9 cut(s)	101, 233, 535, 1072, 1189, 2077, 2256, 2270, 2935
Bst6I	CTCTTC	6 cut(s)	82, 834, 1455, 1728, 2274, 2748
BstACI	GRCGYC	2 cut(s)	182, 290
BstAFI	CTTAAG	1 cut(s)	3029
BstAPI	GCANNNNNTGC	1 cut(s)	2441
BstBAI	YACGTR	1 cut(s)	2353
BstC8I	GCNNGC	8 cut(s)	22, 238, 242, 459, 813, 1750, 1869, 3152
BstDEI	CTNAG	9 cut(s)	338, 990, 1019, 1085, 1125, 1358, 2316, 2871, 3192
BstDSI	CCRYGG	3 cut(s)	190, 513, 928
BstENI	CCTNNNNNAGG	1 cut(s)	1545
BstFNI	CGCG	4 cut(s)	115, 257, 675, 1276
BstH2I	RGCGCY	1 cut(s)	293
BstHHI	GCGC	3 cut(s)	117, 292, 2016
BstMAI	GTCTC	5 cut(s)	682, 807, 2077, 2319, 2867
BstMCI	CGRYCG	3 cut(s)	103, 196, 335
BstNI	CCWGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
BstNSI	RCATGY	6 cut(s)	461, 815, 1172, 2387, 2827, 3189
BstSCI	CCNGG	6 cut(s)	598, 652, 805, 1540, 2151, 3072
BstSFI	CTRYAG	3 cut(s)	423, 1923, 3129
BstSLI	GKGCMC	3 cut(s)	928, 2950, 3200
BstUI	CGCG	4 cut(s)	115, 257, 675, 1276
BstV2I	GAAGAC	3 cut(s)	622, 1046, 2250
BstX2I	RGATCY	3 cut(s)	690, 986, 1712
BstYI	RGATCY	3 cut(s)	690, 986, 1712
BstZI	CGGCCG	2 cut(s)	193, 332
BsuRI	GGCC	5 cut(s)	5, 134, 195, 334, 2405
BtgI	CCRYGG	3 cut(s)	190, 513, 928
BtgZI	GCGATG	1 cut(s)	1257
BtsI	GCAGTG	1 cut(s)	928
BtsIMutI	CAGTG	6 cut(s)	238, 905, 928, 1798, 2102, 2266
BveI	ACCTGC	1 cut(s)	1851
Cac8I	GCNNGC	8 cut(s)	22, 238, 242, 459, 813, 1750, 1869, 3152
CaiI	CAGNNNCTG	2 cut(s)	1501, 2237
CciI	TCATGA	2 cut(s)	1387, 2662
CfoI	GCGC	3 cut(s)	117, 292, 2016
Cfr10I	RCCGGY	1 cut(s)	3150
Cfr13I	GGNCC	5 cut(s)	168, 1333, 1619, 1676, 2600
CseI	GACGC	3 cut(s)	190, 246, 664
Csp6I	GTAC	9 cut(s)	214, 379, 478, 531, 765, 1755, 2590, 2761, 3210
CviQI	GTAC	9 cut(s)	214, 379, 478, 531, 765, 1755, 2590, 2761, 3210
DdeI	CTNAG	9 cut(s)	338, 990, 1019, 1085, 1125, 1358, 2316, 2871, 3192
DinI	GGCGCC	1 cut(s)	291
DraI	TTTAAA	2 cut(s)	1435, 2679
EaeI	YGGCCR	3 cut(s)	193, 332, 2403
EagI	CGGCCG	2 cut(s)	193, 332
Eam1104I	CTCTTC	6 cut(s)	82, 834, 1455, 1728, 2274, 2748
EarI	CTCTTC	6 cut(s)	82, 834, 1455, 1728, 2274, 2748
EciI	GGCGGA	1 cut(s)	290
Ecl136II	GAGCTC	3 cut(s)	996, 1738, 3064
EclXI	CGGCCG	2 cut(s)	193, 332
Eco130I	CCWWGG	3 cut(s)	294, 513, 928
Eco24I	GRGCYC	5 cut(s)	22, 165, 998, 1740, 3066
Eco32I	GATATC	1 cut(s)	1975
Eco47I	GGWCC	5 cut(s)	168, 1333, 1619, 1676, 2600
Eco52I	CGGCCG	2 cut(s)	193, 332
Eco53kI	GAGCTC	3 cut(s)	996, 1738, 3064
Eco57I	CTGAAG	3 cut(s)	616, 2214, 2298
Eco72I	CACGTG	1 cut(s)	2353
Eco88I	CYCGRG	3 cut(s)	164, 284, 2529
EcoICRI	GAGCTC	3 cut(s)	996, 1738, 3064
EcoNI	CCTNNNNNAGG	1 cut(s)	1545
EcoO109I	RGGNCCY	1 cut(s)	168
EcoRII	CCWGG	6 cut(s)	598, 652, 805, 1540, 2151, 3072
EcoRV	GATATC	1 cut(s)	1975
EcoT14I	CCWWGG	3 cut(s)	294, 513, 928
EcoT22I	ATGCAT	4 cut(s)	436, 582, 1273, 2053
EcoT38I	GRGCYC	5 cut(s)	22, 165, 998, 1740, 3066
EgeI	GGCGCC	1 cut(s)	291
EheI	GGCGCC	1 cut(s)	291
ErhI	CCWWGG	3 cut(s)	294, 513, 928
Esp3I	CGTCTC	1 cut(s)	682
FalI	AAGNNNNNCTT	4 cut(s)	614, 646, 3000, 3032
FaqI	GGGAC	2 cut(s)	45, 154
FauI	CCCGC	4 cut(s)	29, 1142, 1771, 2852
FbaI	TGATCA	3 cut(s)	93, 586, 1351
FriOI	GRGCYC	5 cut(s)	22, 165, 998, 1740, 3066
FspBI	CTAG	5 cut(s)	980, 1631, 2507, 2547, 2804
GlaI	GCGC	3 cut(s)	116, 291, 2015
GsaI	CCCAGC	2 cut(s)	1563, 1748
GsuI	CTGGAG	6 cut(s)	174, 582, 855, 1893, 2135, 2366
HaeII	RGCGCY	1 cut(s)	293
HaeIII	GGCC	5 cut(s)	5, 134, 195, 334, 2405
HapII	CCGG	3 cut(s)	46, 2584, 3151
HgaI	GACGC	3 cut(s)	190, 246, 664
HhaI	GCGC	3 cut(s)	117, 292, 2016
Hin1I	GRCGYC	2 cut(s)	182, 290
Hin6I	GCGC	3 cut(s)	115, 290, 2014
HinP1I	GCGC	3 cut(s)	115, 290, 2014
HindIII	AAGCTT	3 cut(s)	1127, 1508, 2412
HpaII	CCGG	3 cut(s)	46, 2584, 3151
Hpy166II	GTNNAC	3 cut(s)	478, 1784, 3179
Hpy8I	GTNNAC	3 cut(s)	478, 1784, 3179
Hpy99I	CGWCG	2 cut(s)	142, 251
HpyCH4III	ACNGT	9 cut(s)	101, 233, 535, 1072, 1189, 2077, 2256, 2270, 2935
HpyCH4IV	ACGT	2 cut(s)	1969, 2352
HpyF3I	CTNAG	9 cut(s)	338, 990, 1019, 1085, 1125, 1358, 2316, 2871, 3192
HpySE526I	ACGT	2 cut(s)	1969, 2352
Hsp92I	GRCGYC	2 cut(s)	182, 290
HspAI	GCGC	3 cut(s)	115, 290, 2014
KasI	GGCGCC	1 cut(s)	289
KflI	GGGWCCC	1 cut(s)	168
KroI	GCCGGC	1 cut(s)	3150
KroNI	GCCGGC	1 cut(s)	3152
Ksp22I	TGATCA	3 cut(s)	93, 586, 1351
LguI	GCTCTTC	2 cut(s)	834, 1728
MaeI	CTAG	5 cut(s)	980, 1631, 2507, 2547, 2804
MaeII	ACGT	2 cut(s)	1969, 2352
MbiI	CCGCTC	2 cut(s)	198, 2218
MfeI	CAATTG	2 cut(s)	2040, 2159
MflI	RGATCY	3 cut(s)	690, 986, 1712
Mly113I	GGCGCC	1 cut(s)	290
MlyI	GAGTC	3 cut(s)	1060, 1186, 1661
MmeI	TCCRAC	4 cut(s)	83, 749, 835, 1398
Mph1103I	ATGCAT	4 cut(s)	436, 582, 1273, 2053
MroNI	GCCGGC	1 cut(s)	3150
MslI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2828, 2898, 3184
MspA1I	CMGCKG	2 cut(s)	337, 1748
MspCI	CTTAAG	1 cut(s)	3029
MspI	CCGG	3 cut(s)	46, 2584, 3151
MspR9I	CCNGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
MunI	CAATTG	2 cut(s)	2040, 2159
Mva1269I	GAATGC	1 cut(s)	2503
MvaI	CCWGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
MvnI	CGCG	4 cut(s)	115, 257, 675, 1276
NaeI	GCCGGC	1 cut(s)	3152
NarI	GGCGCC	1 cut(s)	290
NcoI	CCATGG	2 cut(s)	513, 928
NgoMIV	GCCGGC	1 cut(s)	3150
NlaIV	GGNNCC	5 cut(s)	169, 170, 291, 1621, 1891
NmeAIII	GCCGAG	2 cut(s)	110, 210
NmuCI	GTSAC	8 cut(s)	178, 316, 370, 550, 890, 1189, 1653, 2476
NsiI	ATGCAT	4 cut(s)	436, 582, 1273, 2053
NspI	RCATGY	6 cut(s)	461, 815, 1172, 2387, 2827, 3189
PaeI	GCATGC	2 cut(s)	461, 815
PaeR7I	CTCGAG	1 cut(s)	284
PagI	TCATGA	2 cut(s)	1387, 2662
PciI	ACATGT	3 cut(s)	1168, 2383, 2823
PciSI	GCTCTTC	2 cut(s)	834, 1728
PcsI	WCGNNNNNNNCGW	3 cut(s)	137, 198, 387
PctI	GAATGC	1 cut(s)	2503
PdiI	GCCGGC	1 cut(s)	3152
PflFI	GACNNNGTC	1 cut(s)	1825
PflMI	CCANNNNNTGG	1 cut(s)	2609
PfoI	TCCNGGA	2 cut(s)	1540, 2151
PleI	GAGTC	3 cut(s)	1059, 1186, 1660
PluTI	GGCGCC	1 cut(s)	293
PmaCI	CACGTG	1 cut(s)	2353
PmlI	CACGTG	1 cut(s)	2353
PpsI	GAGTC	3 cut(s)	1059, 1186, 1660
Ppu21I	YACGTR	1 cut(s)	2353
PpuMI	RGGWCCY	1 cut(s)	168
PscI	ACATGT	3 cut(s)	1168, 2383, 2823
Psp124BI	GAGCTC	3 cut(s)	998, 1740, 3066
Psp5II	RGGWCCY	1 cut(s)	168
Psp6I	CCWGG	6 cut(s)	598, 652, 805, 1540, 2151, 3072
PspCI	CACGTG	1 cut(s)	2353
PspFI	CCCAGC	2 cut(s)	1559, 1744
PspGI	CCWGG	6 cut(s)	598, 652, 805, 1540, 2151, 3072
PspN4I	GGNNCC	5 cut(s)	169, 170, 291, 1621, 1891
PspPI	GGNCC	5 cut(s)	168, 1333, 1619, 1676, 2600
PspPPI	RGGWCCY	1 cut(s)	168
PspXI	VCTCGAGB	1 cut(s)	284
PsrI	GAACNNNNNNTAC	2 cut(s)	3160, 3192
PstI	CTGCAG	1 cut(s)	3133
PstNI	CAGNNNCTG	2 cut(s)	1501, 2237
PsuI	RGATCY	3 cut(s)	690, 986, 1712
PsyI	GACNNNGTC	1 cut(s)	1825
PvuII	CAGCTG	1 cut(s)	1748
RsaI	GTAC	9 cut(s)	215, 380, 479, 532, 766, 1756, 2591, 2762, 3211
RsaNI	GTAC	9 cut(s)	214, 379, 478, 531, 765, 1755, 2590, 2761, 3210
RseI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2828, 2898, 3184
SacI	GAGCTC	3 cut(s)	998, 1740, 3066
SapI	GCTCTTC	2 cut(s)	834, 1728
Sau96I	GGNCC	5 cut(s)	168, 1333, 1619, 1676, 2600
SchI	GAGTC	3 cut(s)	1060, 1186, 1661
ScrFI	CCNGG	6 cut(s)	600, 654, 807, 1542, 2153, 3074
SfcI	CTRYAG	3 cut(s)	423, 1923, 3129
SfoI	GGCGCC	1 cut(s)	291
Sfr274I	CTCGAG	1 cut(s)	284
SinI	GGWCC	5 cut(s)	168, 1333, 1619, 1676, 2600
SlaI	CTCGAG	1 cut(s)	284
SmiMI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2828, 2898, 3184
SmlI	CTYRAG	5 cut(s)	284, 686, 1773, 2720, 3029
SmoI	CTYRAG	5 cut(s)	284, 686, 1773, 2720, 3029
SphI	GCATGC	2 cut(s)	461, 815
SspDI	GGCGCC	1 cut(s)	289
SspMI	CTAG	5 cut(s)	980, 1631, 2507, 2547, 2804
SstI	GAGCTC	3 cut(s)	998, 1740, 3066
StyD4I	CCNGG	6 cut(s)	598, 652, 805, 1540, 2151, 3072
StyI	CCWWGG	3 cut(s)	294, 513, 928
TaaI	ACNGT	9 cut(s)	101, 233, 535, 1072, 1189, 2077, 2256, 2270, 2935
TaiI	ACGT	2 cut(s)	1972, 2355
TaqI	TCGA	7 cut(s)	71, 125, 285, 886, 998, 1117, 3037
TaqII	GACCGA	1 cut(s)	1321
TatI	WGTACW	3 cut(s)	1754, 2589, 2760
TauI	GCSGC	3 cut(s)	198, 337, 2221
TscAI	CASTG	6 cut(s)	238, 912, 928, 1798, 2102, 2273
TseFI	GTSAC	8 cut(s)	178, 316, 370, 550, 890, 1189, 1653, 2476
Tsp45I	GTSAC	8 cut(s)	178, 316, 370, 550, 890, 1189, 1653, 2476
TspDTI	ATGAA	8 cut(s)	69, 1308, 1746, 1995, 2346, 3009, 3056, 3065
TspGWI	ACGGA	3 cut(s)	166, 960, 3096
TspRI	CASTG	6 cut(s)	238, 912, 928, 1798, 2102, 2273
Tth111I	GACNNNGTC	1 cut(s)	1825
Van91I	CCANNNNNTGG	1 cut(s)	2609
Vha464I	CTTAAG	1 cut(s)	3029
VpaK11BI	GGWCC	5 cut(s)	168, 1333, 1619, 1676, 2600
XagI	CCTNNNNNAGG	1 cut(s)	1545
XapI	RAATTY	6 cut(s)	662, 736, 2001, 2307, 2461, 2744
XbaI	TCTAGA	3 cut(s)	1630, 2546, 2803
XceI	RCATGY	6 cut(s)	461, 815, 1172, 2387, 2827, 3189
XhoI	CTCGAG	1 cut(s)	284
XspI	CTAG	5 cut(s)	980, 1631, 2507, 2547, 2804
Zsp2I	ATGCAT	4 cut(s)	436, 582, 1273, 2053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.