Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0004310

Physical Location & Seq

Genomic Coordinates Forward (+)

48800513 .. 48810958

Gene Structure 10446 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00201230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGGCCTCGTCCTCCCGAGCAAGGAGCAGCTCGCCATTCTCGTACCGCAAGCCTTCAAGTCCCTACTCCTCGACCTCCTCCTCGTCCTCGCTGATCAACGGCCGCGCTCTCATTCCGCGCTCCTGCTCCAGCTCAGCGACGTCGTTTTACAACTCCGGAGGTGGACTCGGTCCCCGATCCGCCACGCCGAGCCACTCCCGCTCCGAATCAATGTACCAAGGCTCGCGGTCCTACGGCGGTGTCTCGCCCGTGGGGTTCGCCGCGGAGGACCTTATGACCGAGATTGTGGATGCGCCGAGGTCTGGGGATAGCATATCGGTCACGATTCGGTTTCGGCCTCTCAGCGAGAGGGAGTATCAGAGAGGGGATGAGGTCACTTGGTATGCAGATGGTGATAAGATTGTGCGGAATGCGTACAATCCAGTTACAGCCTATGCATTCGATAAAGTTTTTGGACAGGATACGATTTCGCAAGAGGTGTATGAGGTTGCTGCCAAACCTGTGGTCAAAGCTGCCATGGAAGGTGTCAATGGCACTGTTTTTGCCTATGGTGTGACAAGTAGTGGGAAGACGCACACTATGCATGGAGATCAAAATGCTCCTGGTGTTATACCATTGGCCATAAAAGATGTATTCAGTATGATCCAAGATACCCCAGGAAGGGAGTTTTTACTTCGTGTCTCATATCTTGAGATCTACAATGAGGTGATAAATGATTTGCTTGACCCAACAGGTCAAAATTTGCGTGTTAGAGAAGATTCACAGGGTACATATGTTGAGGGTATAAAAGAAGAAGTTGTTCTGTCTCCTGGGCATGCACTTTCGTTTATTGCTGCTGGAGAAGAGCACCGTCATGTTGGATCAAACAATTATAATTTGTTCAGTAGTAGAAGTCACACCATATTTACACTGATGATTGAAAGTAGTGCCCATGGGGATGAATATGATGGAGTGATCTTCTCTCAACTTAACTTGATTGATCTAGCTGGATCGGAGAGCTCTAAAACCGAAACAACTGGACTAAGAAGAAAGGAAGGATCTTACATTAACAAGAGCCTTTTAACTCTTGGAACTGTAATTGGGAAGCTGAGTGAGGGAAAGGCATCCCATGTTCCATATCGAGATTCTAAGCTCACCCGCCTTTTACAATCTTCGCTAAGTGGGCATGGACATGTTTCGCTCATTTGCACGGTTACTCCTGCATCAAGTAGCATGGAGGAAACCCATAATACATTGAAGTTCGCAAGCAGAGCAAAGCGAGTAGAGATCTACGCATCACGTAATAAGGTGCGCTACATTTTACTATTGGAGGAAGAAGAGGAAGCAAAGGCTGCTCTTATGAGTAGAATCCAGAGGCTGACGAAGCTCATTCTTGTTTCATCAAAAAATACCATACCTGGATGTTTGAGTGATATTCCCACCCACCAACGACGTTATTCTGTTGGTGAGGACGATAAAACGGAAGTAGTGCGAGATGGGTCCTTGCTAGTAGAAAGTGAGAACCAGGACTCACCTTCTTCTGCCTCTGGTGTCCCATCAGATCTGTCTTATGATATTAGACACAAAAGATCTTCCAGCAGGTGGAATGAAGAGCTCTCACCAGCTGGCAGTACAATTACAGAATCAACTCAAGCTGGGGAACTTATCAGTGGTTCGAAACTGACAACAGGAGGGATGACAATGACAGATCATATAGACCTGCTTGTCGAGCAAGTAAAGATGCTTGCTGGAGAGATAGCACTTGGCACCAGCACCTTGAAGCGAATGGTGGAGCAGTCTGTAAATGACCCTGATAGCTCCAAAGTTCAAATTGAGAACTTGGAACGTGATATCCATGAAAAGAGAAGGCAAATGAGGGTTTTAGAACAGCGCATCAATGAAAGTGGTGAGGCTTCAGTCTCTAATGCATCTTTGGTTGAGATGCAGCAGACAGTTAAGAGACTGATGACACAGTGCGATGAGAAGGGGTTTGAGTTGGAAATAAAATCAGCAGACAATCGTATTCTTCAAGAGCAACTGCAAAATAAGTTAGAGCGGTGCCTAGCTTCAGGGTTTTCCGAGCACGGTGTGTCTGAAGAGTATGTTGATGAATTGAGAAAGAAAATCCAGTCTCAGGAGATTGAGAACGAAAGACTAAAACTGGAGCATGTCCAGTTCTCAGAGGAGAATAGTGGGTTACATGTGCAAAATCAGAAACTGGCTGAAGAAGCTTCTTATGCAAAGGAACTGGCATCTGCTGCTGCTGTTGAGTTGAAAAACTTGGCCGGTGAAGTGACAAAACTGTCATTACAGAGTGCTAAACTGGAGAAAGAGTTGTTGGCTGCTCGTGAGCTAGCCAATAATTCTAGAAGTTCTAACATGCATCCTGGTAATGGCGTCAACCGCAAGTACAATGAGGGATCAAAACCTGGGAGGAAAGGGAGGCTTTCTGGTCGTGCTAATGATATGCTGTCTGAGGACTTTGAGTCTTGGAATCTTGATCCTGATGATTTAAGGATGGAACTCCAGGCTAGGAAACAGCGAGAGGCAGCTCTTGAGGCTGCTCTGTCTGAAAAGGAATTTGTAGAGGAGGAATACAGGAAAAAGGTTGAAGATGCAAAGAAAAGAGAAGAAGCTCTGGAAAATGACTTGGCAAACATGTGGGTTCTGGTTGCAAAATTAAAGAAAGATGGTGGAGCTATCCCTGAGACAACCACAGAAGAAAGACACAATGATGCCATGGAAACCAATAATGGTTTTAAAGCAAATGACAGTGAAAGTAATACTATCCCCAAAGAGAGACAAGCGTTGGATGTTTCAAAACCAGCTAATGATGAAGTTCGCACCGAAGAACCCCTCGTTCTTCGCCTGAAGGCTCGAATGCAAGAGATGAAGGAAAAAGAGCTCAAACACCAGGGAAATGGTGATGCCAATTCCCATCTCTGTAAATATGTAAATCGTGTTCACTTGCTTGTTCCGAGTGCCCAATTTGTCGTACAAAGATTGCAGATAGGCTTTTTGCATTTACTTCGTGATGCTGTTTTATGCCCTCAAATAAAGGGCTTTGTCATCATTTGCCAGTTTGCAATTTTCCGTGATATAGGGGTGCAATTCTTTAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031347 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0065007 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin zf-C3HC4_3

Protein Analysis

1033

Amino Acids

114.56

Weight (kDa)

5.8

Isoelectric Point (pI)

51.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	110 - 421	5.7e-105	Kinesin motor domain
Microtub_bd	PF16796	134 - 241	5e-19	Microtubule binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21380 AT2G21380 AT4G39050
fragaria_vesca	FvH4_2g10080
malus_domestica	MD05G1058100.v1.1 MD10G1065900.v1.1
prunus_persica	Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1
pyrus_communis	pycom05g04920 pycom10g05130 pycom10g05140
rosa_chinensis	RchiOBHm_Chr6g0267011
rosa_laevigata	RLG00000014051 RLG00000027737 RLG00000037008
rosa_multiflora	Rmu_co8233357.1_g000001 Rmu_sc0013804.1_g000010 Rmu_sc0017071.1_g000008 Rmu_sc0037796.1_g000001 Rmu_sc0041080.1_g000001 Rmu_ssc0000341.1_g000028
rosa_roxburghii	Rroxscaffold_2G00112220 Rroxscaffold_7G00201230
rosa_rugosa	Rorug06G0028400 Rorug06G0028400 Rorug06G0028400
rosa_samantha	Rh1AG191400 Rh1CG176500 Rh6AG150000 Rh6CG146100
rosa_wichuraiana	Rw6G012990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	873
AarI	CACCTGC	1 cut(s)	1569
AasI	GACNNNNNNGTC	2 cut(s)	1703, 2281
AatII	GACGTC	1 cut(s)	143
Acc36I	ACCTGC	2 cut(s)	1569, 1707
AccB1I	GGYRCC	2 cut(s)	1745, 2037
AccB7I	CCANNNNNTGG	1 cut(s)	1581
AccBSI	CCGCTC	2 cut(s)	201, 2035
AccII	CGCG	4 cut(s)	105, 118, 226, 263
AccIII	TCCGGA	1 cut(s)	155
AclWI	GGATC	7 cut(s)	171, 637, 868, 997, 1045, 2407, 2474
AcoI	YGGCCR	3 cut(s)	100, 618, 2262
AcsI	RAATTY	2 cut(s)	739, 2558
AcuI	CTGAAG	5 cut(s)	1878, 2031, 2094, 2223, 2870
AcyI	GRCGYC	2 cut(s)	140, 2376
AdeI	CACNNNGTG	1 cut(s)	2068
AfaI	GTAC	7 cut(s)	44, 215, 416, 769, 1612, 2390, 2976
AfiI	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1581, 2113, 2372
AflIII	ACRYGT	3 cut(s)	1171, 2179, 2637
AgsI	TTSAA	9 cut(s)	57, 920, 1237, 1759, 1808, 2009, 2254, 2591, 2799
AhdI	GACNNNNNGTC	1 cut(s)	2464
AjnI	CCWGG	9 cut(s)	601, 655, 808, 1396, 1503, 2365, 2407, 2505, 2892
AjuI	GAANNNNNNNTTGG	2 cut(s)	2300, 2332
AloI	GAACNNNNNNTCC	2 cut(s)	1815, 1847
Alw21I	GWGCWC	5 cut(s)	849, 1001, 1596, 2064, 2886
Alw26I	GTCTC	8 cut(s)	247, 685, 810, 1903, 1933, 2115, 2681, 2773
AlwI	GGATC	7 cut(s)	171, 637, 868, 997, 1045, 2407, 2474
AlwNI	CAGNNNCTG	2 cut(s)	1357, 2197
Ama87I	CYCGRG	1 cut(s)	15
Aor13HI	TCCGGA	1 cut(s)	155
AoxI	GGCC	5 cut(s)	3, 100, 335, 618, 2262
ApoI	RAATTY	2 cut(s)	739, 2558
ArsI	GACNNNNNNTTYG	2 cut(s)	489, 521
Asp700I	GAANNNNTTC	1 cut(s)	798
AspLEI	GCGC	5 cut(s)	107, 120, 295, 1293, 1872
AspS9I	GGNCC	4 cut(s)	170, 228, 268, 1479
AsuHPI	GGTGA	9 cut(s)	404, 718, 1126, 1457, 1503, 1590, 1898, 2279, 2915
AsuII	TTCGAA	1 cut(s)	1655
AsuNHI	GCTAGC	1 cut(s)	2332
AvaI	CYCGRG	1 cut(s)	15
AvaII	GGWCC	4 cut(s)	170, 228, 268, 1479
BaeGI	GKGCMC	2 cut(s)	931, 2965
BaeI	ACNNNNGTAYC	2 cut(s)	759, 792
BalI	TGGCCA	1 cut(s)	620
BanI	GGYRCC	2 cut(s)	1745, 2037
BanII	GRGCYC	3 cut(s)	1001, 1596, 2886
BauI	CACGAG	1 cut(s)	2325
BbsI	GAAGAC	1 cut(s)	575
Bbv12I	GWGCWC	5 cut(s)	849, 1001, 1596, 2064, 2886
BccI	CCATC	7 cut(s)	383, 941, 1469, 1543, 2491, 2663, 2925
BceAI	ACGGC	2 cut(s)	115, 250
BcgI	CGANNNNNNTGC	2 cut(s)	2990, 3024
BciT130I	CCWGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
BciVI	GTATCC	1 cut(s)	454
BclI	TGATCA	1 cut(s)	93
BcoDI	GTCTC	8 cut(s)	247, 685, 810, 1903, 1933, 2115, 2681, 2773
BfaI	CTAG	6 cut(s)	983, 1487, 2042, 2333, 2346, 2511
BfuAI	ACCTGC	2 cut(s)	1569, 1707
BfuI	GTATCC	1 cut(s)	454
BglII	AGATCT	4 cut(s)	693, 1266, 1540, 1568
BlpI	GCTNAGC	1 cut(s)	133
Bme1390I	CCNGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
Bme18I	GGWCC	4 cut(s)	170, 228, 268, 1479
BmeRI	GACNNNNNGTC	1 cut(s)	2464
BmeT110I	CYCGRG	1 cut(s)	15
BmgT120I	GGNCC	4 cut(s)	170, 228, 268, 1479
BmiI	GGNNCC	4 cut(s)	172, 1480, 1747, 2039
BmrFI	CCNGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
BmtI	GCTAGC	1 cut(s)	2336
BpiI	GAAGAC	1 cut(s)	575
BplI	GAGNNNNNCTC	2 cut(s)	150, 182
BpmI	CTGGAG	6 cut(s)	112, 858, 1749, 2162, 2324, 2489
Bpu1102I	GCTNAGC	1 cut(s)	133
Bpu14I	TTCGAA	1 cut(s)	1655
BpuEI	CTTGAG	3 cut(s)	710, 1614, 2555
BsaAI	YACGTR	1 cut(s)	1280
BsaHI	GRCGYC	2 cut(s)	140, 2376
BsaWI	WCCGGW	1 cut(s)	155
BsaXI	ACNNNNNCTCC	6 cut(s)	185, 215, 1180, 1210, 2297, 2327
Bsc4I	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1581, 2113, 2372
Bse118I	RCCGGY	1 cut(s)	2264
Bse1I	ACTGG	9 cut(s)	422, 1021, 2107, 2145, 2152, 2202, 2232, 2307, 3058
BseAI	TCCGGA	1 cut(s)	155
BseBI	CCWGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
BseGI	GGATG	9 cut(s)	295, 373, 943, 1103, 1406, 1680, 2362, 2502, 2797
BseLI	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1581, 2113, 2372
BseMII	CTCAG	7 cut(s)	147, 355, 1079, 2126, 2172, 2445, 2676
BseNI	ACTGG	9 cut(s)	422, 1021, 2107, 2145, 2152, 2202, 2232, 2307, 3058
BseRI	GAGGAG	5 cut(s)	58, 67, 70, 2177, 2582
BseSI	GKGCMC	2 cut(s)	931, 2965
BseX3I	CGGCCG	1 cut(s)	100
BseYI	CCCAGC	1 cut(s)	1634
Bsh1236I	CGCG	4 cut(s)	105, 118, 226, 263
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	5 cut(s)	5, 102, 337, 620, 2264
BshNI	GGYRCC	2 cut(s)	1745, 2037
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	5 cut(s)	849, 1001, 1596, 2064, 2886
BsiHKCI	CYCGRG	1 cut(s)	15
BsiSI	CCGG	2 cut(s)	156, 2265
BslFI	GGGAC	3 cut(s)	45, 156, 1517
BslI	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1581, 2113, 2372
BsmAI	GTCTC	8 cut(s)	247, 685, 810, 1903, 1933, 2115, 2681, 2773
BsmFI	GGGAC	3 cut(s)	45, 156, 1517
BsmI	GAATGC	3 cut(s)	415, 437, 2865
BsnI	GGCC	5 cut(s)	5, 102, 337, 620, 2264
BsoBI	CYCGRG	1 cut(s)	15
Bsp119I	TTCGAA	1 cut(s)	1655
Bsp1286I	GDGCHC	7 cut(s)	849, 931, 1001, 1596, 2064, 2886, 2965
Bsp13I	TCCGGA	1 cut(s)	155
Bsp1720I	GCTNAGC	1 cut(s)	133
Bsp19I	CCATGG	3 cut(s)	516, 931, 2718
BspANI	GGCC	5 cut(s)	5, 102, 337, 620, 2264
BspCNI	CTCAG	7 cut(s)	146, 354, 1080, 2125, 2171, 2446, 2677
BspEI	TCCGGA	1 cut(s)	155
BspFNI	CGCG	4 cut(s)	105, 118, 226, 263
BspLI	GGNNCC	4 cut(s)	172, 1480, 1747, 2039
BspMI	ACCTGC	2 cut(s)	1569, 1707
BspOI	GCTAGC	1 cut(s)	2336
BspPI	GGATC	7 cut(s)	171, 637, 868, 997, 1045, 2407, 2474
BspQI	GCTCTTC	2 cut(s)	837, 1584
BspT104I	TTCGAA	1 cut(s)	1655
BspT107I	GGYRCC	2 cut(s)	1745, 2037
BsrBI	CCGCTC	2 cut(s)	201, 2035
BsrFI	RCCGGY	1 cut(s)	2264
BsrI	ACTGG	9 cut(s)	422, 1021, 2107, 2145, 2152, 2202, 2232, 2307, 3058
BssAI	RCCGGY	1 cut(s)	2264
BssNI	GRCGYC	2 cut(s)	140, 2376
BssSI	CACGAG	1 cut(s)	2325
BssT1I	CCWWGG	4 cut(s)	217, 516, 931, 2718
Bst2BI	CACGAG	1 cut(s)	2325
Bst2UI	CCWGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
Bst4CI	ACNGT	9 cut(s)	538, 851, 1075, 1192, 1933, 1953, 2066, 2283, 2753
Bst6I	CTCTTC	4 cut(s)	837, 1311, 1584, 2070
BstACI	GRCGYC	2 cut(s)	140, 2376
BstAPI	GCANNNNNTGC	3 cut(s)	580, 1331, 2237
BstBAI	YACGTR	1 cut(s)	1280
BstBI	TTCGAA	1 cut(s)	1655
BstC8I	GCNNGC	8 cut(s)	32, 50, 224, 816, 1246, 1606, 1725, 2334
BstDSI	CCRYGG	5 cut(s)	249, 261, 516, 931, 2718
BstF5I	GGATG	9 cut(s)	295, 373, 943, 1103, 1406, 1680, 2362, 2502, 2797
BstFNI	CGCG	4 cut(s)	105, 118, 226, 263
BstHHI	GCGC	5 cut(s)	107, 120, 295, 1293, 1872
BstMAI	GTCTC	8 cut(s)	247, 685, 810, 1903, 1933, 2115, 2681, 2773
BstMCI	CGRYCG	1 cut(s)	103
BstNI	CCWGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
BstNSI	RCATGY	6 cut(s)	818, 1175, 2150, 2183, 2362, 2641
BstSCI	CCNGG	9 cut(s)	601, 655, 808, 1396, 1503, 2365, 2407, 2505, 2892
BstSLI	GKGCMC	2 cut(s)	931, 2965
BstUI	CGCG	4 cut(s)	105, 118, 226, 263
BstV2I	GAAGAC	1 cut(s)	575
BstX2I	RGATCY	5 cut(s)	693, 1037, 1266, 1540, 1568
BstXI	CCANNNNNNTGG	2 cut(s)	502, 2900
BstYI	RGATCY	5 cut(s)	693, 1037, 1266, 1540, 1568
BstZI	CGGCCG	1 cut(s)	100
BsuI	GTATCC	1 cut(s)	454
BsuRI	GGCC	5 cut(s)	5, 102, 337, 620, 2264
BtgI	CCRYGG	5 cut(s)	249, 261, 516, 931, 2718
BtgZI	GCGATG	1 cut(s)	1971
BtsCI	GGATG	9 cut(s)	295, 373, 943, 1103, 1406, 1680, 2362, 2502, 2797
BtsIMutI	CAGTG	5 cut(s)	534, 908, 1654, 1958, 2758
BveI	ACCTGC	2 cut(s)	1569, 1707
Cac8I	GCNNGC	8 cut(s)	32, 50, 224, 816, 1246, 1606, 1725, 2334
CaiI	CAGNNNCTG	2 cut(s)	1357, 2197
CfoI	GCGC	5 cut(s)	107, 120, 295, 1293, 1872
Cfr10I	RCCGGY	1 cut(s)	2264
Cfr13I	GGNCC	4 cut(s)	170, 228, 268, 1479
Cfr42I	CCGCGG	1 cut(s)	264
CseI	GACGC	2 cut(s)	580, 2365
Csp6I	GTAC	7 cut(s)	43, 214, 415, 768, 1611, 2389, 2975
CspCI	CAANNNNNGTGG	2 cut(s)	1864, 1899
CviQI	GTAC	7 cut(s)	43, 214, 415, 768, 1611, 2389, 2975
DraI	TTTAAA	1 cut(s)	2740
DraIII	CACNNNGTG	1 cut(s)	2068
DrdI	GACNNNNNNGTC	2 cut(s)	1703, 2281
DriI	GACNNNNNGTC	1 cut(s)	2464
DseDI	GACNNNNNNGTC	2 cut(s)	1703, 2281
EaeI	YGGCCR	3 cut(s)	100, 618, 2262
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	4 cut(s)	837, 1311, 1584, 2070
Eam1105I	GACNNNNNGTC	1 cut(s)	2464
EarI	CTCTTC	4 cut(s)	837, 1311, 1584, 2070
EciI	GGCGGA	1 cut(s)	169
Ecl136II	GAGCTC	3 cut(s)	999, 1594, 2884
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	4 cut(s)	217, 516, 931, 2718
Eco24I	GRGCYC	3 cut(s)	1001, 1596, 2886
Eco32I	GATATC	1 cut(s)	1831
Eco47I	GGWCC	4 cut(s)	170, 228, 268, 1479
Eco52I	CGGCCG	1 cut(s)	100
Eco53kI	GAGCTC	3 cut(s)	999, 1594, 2884
Eco57I	CTGAAG	5 cut(s)	1878, 2031, 2094, 2223, 2870
Eco88I	CYCGRG	1 cut(s)	15
EcoICRI	GAGCTC	3 cut(s)	999, 1594, 2884
EcoO109I	RGGNCCY	2 cut(s)	268, 1479
EcoRII	CCWGG	9 cut(s)	601, 655, 808, 1396, 1503, 2365, 2407, 2505, 2892
EcoRV	GATATC	1 cut(s)	1831
EcoT14I	CCWWGG	4 cut(s)	217, 516, 931, 2718
EcoT22I	ATGCAT	4 cut(s)	439, 585, 1909, 2364
EcoT38I	GRGCYC	3 cut(s)	1001, 1596, 2886
ErhI	CCWWGG	4 cut(s)	217, 516, 931, 2718
FalI	AAGNNNNNCTT	2 cut(s)	2409, 2441
FaqI	GGGAC	3 cut(s)	45, 156, 1517
FauI	CCCGC	2 cut(s)	206, 1145
FauNDI	CATATG	1 cut(s)	772
FbaI	TGATCA	1 cut(s)	93
FokI	GGATG	9 cut(s)	302, 380, 950, 1090, 1413, 1687, 2349, 2509, 2804
FriOI	GRGCYC	3 cut(s)	1001, 1596, 2886
FspBI	CTAG	6 cut(s)	983, 1487, 2042, 2333, 2346, 2511
GlaI	GCGC	5 cut(s)	106, 119, 294, 1292, 1871
GsaI	CCCAGC	1 cut(s)	1638
GsuI	CTGGAG	6 cut(s)	112, 858, 1749, 2162, 2324, 2489
HaeIII	GGCC	5 cut(s)	5, 102, 337, 620, 2264
HapII	CCGG	2 cut(s)	156, 2265
HgaI	GACGC	2 cut(s)	580, 2365
HhaI	GCGC	5 cut(s)	107, 120, 295, 1293, 1872
Hin1I	GRCGYC	2 cut(s)	140, 2376
Hin6I	GCGC	5 cut(s)	105, 118, 293, 1291, 1870
HinP1I	GCGC	5 cut(s)	105, 118, 293, 1291, 1870
HincII	GTYRAC	1 cut(s)	2380
HindII	GTYRAC	1 cut(s)	2380
HindIII	AAGCTT	1 cut(s)	2208
HpaII	CCGG	2 cut(s)	156, 2265
HphI	GGTGA	9 cut(s)	404, 718, 1126, 1457, 1503, 1590, 1898, 2279, 2915
Hpy166II	GTNNAC	3 cut(s)	164, 2380, 2944
Hpy8I	GTNNAC	3 cut(s)	164, 2380, 2944
Hpy99I	CGWCG	3 cut(s)	142, 145, 1434
HpyAV	CCTTC	9 cut(s)	63, 515, 654, 1028, 1524, 1839, 1957, 2845, 2866
HpyCH4III	ACNGT	9 cut(s)	538, 851, 1075, 1192, 1933, 1953, 2066, 2283, 2753
HpyCH4IV	ACGT	4 cut(s)	140, 1279, 1432, 1825
HpySE526I	ACGT	4 cut(s)	140, 1279, 1432, 1825
Hsp92I	GRCGYC	2 cut(s)	140, 2376
HspAI	GCGC	5 cut(s)	105, 118, 293, 1291, 1870
Kpn2I	TCCGGA	1 cut(s)	155
Ksp22I	TGATCA	1 cut(s)	93
KspI	CCGCGG	1 cut(s)	264
LguI	GCTCTTC	2 cut(s)	837, 1584
LmnI	GCTCC	9 cut(s)	24, 125, 131, 206, 604, 1771, 1802, 2143, 2675
MaeI	CTAG	6 cut(s)	983, 1487, 2042, 2333, 2346, 2511
MaeII	ACGT	4 cut(s)	140, 1279, 1432, 1825
MaeIII	GTNAC	8 cut(s)	319, 373, 424, 553, 893, 1192, 2175, 2272
MbiI	CCGCTC	2 cut(s)	201, 2035
MflI	RGATCY	5 cut(s)	693, 1037, 1266, 1540, 1568
MhlI	GDGCHC	7 cut(s)	849, 931, 1001, 1596, 2064, 2886, 2965
MlsI	TGGCCA	1 cut(s)	620
MluNI	TGGCCA	1 cut(s)	620
MlyI	GAGTC	3 cut(s)	159, 1502, 2474
MmeI	TCCRAC	3 cut(s)	838, 1956, 2769
Mox20I	TGGCCA	1 cut(s)	620
Mph1103I	ATGCAT	4 cut(s)	439, 585, 1909, 2364
MroI	TCCGGA	1 cut(s)	155
MroXI	GAANNNNTTC	1 cut(s)	798
MscI	TGGCCA	1 cut(s)	620
MseI	TTAA	8 cut(s)	969, 1047, 1061, 1935, 2492, 2660, 2739, 3096
MslI	CAYNNNNRTG	4 cut(s)	852, 936, 1170, 2712
Msp20I	TGGCCA	1 cut(s)	620
MspA1I	CMGCKG	2 cut(s)	263, 1604
MspI	CCGG	2 cut(s)	156, 2265
MspR9I	CCNGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
Mva1269I	GAATGC	3 cut(s)	415, 437, 2865
MvaI	CCWGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
MvnI	CGCG	4 cut(s)	105, 118, 226, 263
NcoI	CCATGG	3 cut(s)	516, 931, 2718
NdeI	CATATG	1 cut(s)	772
NheI	GCTAGC	1 cut(s)	2332
NlaIV	GGNNCC	4 cut(s)	172, 1480, 1747, 2039
NmeAIII	GCCGAG	2 cut(s)	213, 321
NmuCI	GTSAC	5 cut(s)	319, 373, 553, 893, 2272
NsiI	ATGCAT	4 cut(s)	439, 585, 1909, 2364
NspI	RCATGY	6 cut(s)	818, 1175, 2150, 2183, 2362, 2641
NspV	TTCGAA	1 cut(s)	1655
PaeI	GCATGC	1 cut(s)	818
PaqCI	CACCTGC	1 cut(s)	1569
PciI	ACATGT	3 cut(s)	1171, 2179, 2637
PciSI	GCTCTTC	2 cut(s)	837, 1584
PcsI	WCGNNNNNNNCGW	1 cut(s)	38
PctI	GAATGC	3 cut(s)	415, 437, 2865
PdmI	GAANNNNTTC	1 cut(s)	798
PfeI	GAWTC	7 cut(s)	206, 325, 758, 1124, 1347, 1622, 2473
PflFI	GACNNNGTC	1 cut(s)	168
PflMI	CCANNNNNTGG	1 cut(s)	1581
PleI	GAGTC	3 cut(s)	159, 1502, 2473
PpsI	GAGTC	3 cut(s)	159, 1502, 2473
Ppu21I	YACGTR	1 cut(s)	1280
PpuMI	RGGWCCY	2 cut(s)	268, 1479
PscI	ACATGT	3 cut(s)	1171, 2179, 2637
PsiI	TTATAA	1 cut(s)	873
Psp124BI	GAGCTC	3 cut(s)	1001, 1596, 2886
Psp5II	RGGWCCY	2 cut(s)	268, 1479
Psp6I	CCWGG	9 cut(s)	601, 655, 808, 1396, 1503, 2365, 2407, 2505, 2892
PspFI	CCCAGC	1 cut(s)	1634
PspGI	CCWGG	9 cut(s)	601, 655, 808, 1396, 1503, 2365, 2407, 2505, 2892
PspN4I	GGNNCC	4 cut(s)	172, 1480, 1747, 2039
PspPI	GGNCC	4 cut(s)	170, 228, 268, 1479
PspPPI	RGGWCCY	2 cut(s)	268, 1479
PsrI	GAACNNNNNNTAC	2 cut(s)	2925, 2957
PstNI	CAGNNNCTG	2 cut(s)	1357, 2197
PsuI	RGATCY	5 cut(s)	693, 1037, 1266, 1540, 1568
PsyI	GACNNNGTC	1 cut(s)	168
PvuII	CAGCTG	1 cut(s)	1604
RsaI	GTAC	7 cut(s)	44, 215, 416, 769, 1612, 2390, 2976
RsaNI	GTAC	7 cut(s)	43, 214, 415, 768, 1611, 2389, 2975
RseI	CAYNNNNRTG	4 cut(s)	852, 936, 1170, 2712
SacI	GAGCTC	3 cut(s)	1001, 1596, 2886
SacII	CCGCGG	1 cut(s)	264
SapI	GCTCTTC	2 cut(s)	837, 1584
SaqAI	TTAA	8 cut(s)	969, 1047, 1061, 1935, 2492, 2660, 2739, 3096
Sau96I	GGNCC	4 cut(s)	170, 228, 268, 1479
SchI	GAGTC	3 cut(s)	159, 1502, 2474
ScrFI	CCNGG	9 cut(s)	603, 657, 810, 1398, 1505, 2367, 2409, 2507, 2894
SduI	GDGCHC	7 cut(s)	849, 931, 1001, 1596, 2064, 2886, 2965
Sfr303I	CCGCGG	1 cut(s)	264
SfuI	TTCGAA	1 cut(s)	1655
SgrBI	CCGCGG	1 cut(s)	264
SinI	GGWCC	4 cut(s)	170, 228, 268, 1479
SmiMI	CAYNNNNRTG	4 cut(s)	852, 936, 1170, 2712
SmlI	CTYRAG	3 cut(s)	689, 1629, 2534
SmoI	CTYRAG	3 cut(s)	689, 1629, 2534
SphI	GCATGC	1 cut(s)	818
SspMI	CTAG	6 cut(s)	983, 1487, 2042, 2333, 2346, 2511
SstI	GAGCTC	3 cut(s)	1001, 1596, 2886
StyD4I	CCNGG	9 cut(s)	601, 655, 808, 1396, 1503, 2365, 2407, 2505, 2892
StyI	CCWWGG	4 cut(s)	217, 516, 931, 2718
TaaI	ACNGT	9 cut(s)	538, 851, 1075, 1192, 1933, 1953, 2066, 2283, 2753
TaiI	ACGT	4 cut(s)	143, 1282, 1435, 1828
TaqI	TCGA	6 cut(s)	71, 441, 1120, 1655, 1707, 2857
TaqII	GACCGA	3 cut(s)	158, 293, 307
TatI	WGTACW	2 cut(s)	1610, 2388
TauI	GCSGC	2 cut(s)	105, 263
TfiI	GAWTC	7 cut(s)	206, 325, 758, 1124, 1347, 1622, 2473
Tru1I	TTAA	8 cut(s)	969, 1047, 1061, 1935, 2492, 2660, 2739, 3096
Tru9I	TTAA	8 cut(s)	969, 1047, 1061, 1935, 2492, 2660, 2739, 3096
TscAI	CASTG	5 cut(s)	541, 915, 1654, 1958, 2758
TseFI	GTSAC	5 cut(s)	319, 373, 553, 893, 2272
Tsp45I	GTSAC	5 cut(s)	319, 373, 553, 893, 2272
TspDTI	ATGAA	8 cut(s)	954, 1368, 1602, 1851, 1893, 2103, 2829, 2885
TspGWI	ACGGA	2 cut(s)	1475, 3062
TspRI	CASTG	5 cut(s)	541, 915, 1654, 1958, 2758
Tth111I	GACNNNGTC	1 cut(s)	168
Van91I	CCANNNNNTGG	1 cut(s)	1581
VpaK11BI	GGWCC	4 cut(s)	170, 228, 268, 1479
XapI	RAATTY	2 cut(s)	739, 2558
XbaI	TCTAGA	1 cut(s)	2345
XceI	RCATGY	6 cut(s)	818, 1175, 2150, 2183, 2362, 2641
XmnI	GAANNNNTTC	1 cut(s)	798
XspI	CTAG	6 cut(s)	983, 1487, 2042, 2333, 2346, 2511
ZraI	GACGTC	1 cut(s)	141
Zsp2I	ATGCAT	4 cut(s)	439, 585, 1909, 2364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.