Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0004310

Physical Location & Seq

Genomic Coordinates Forward (+)

5806077 .. 5817308

Gene Structure 11232 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g04920.22

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3258 bp

ATGGCCTCGTCCTCACGAGCCCGCAGCAGCTCGCCTTTCTCTTACCGGAAGCCATCCAGTCCCTACTCCTCCACCTCCTCGTCTGCCGCGTTGATCAACGGTCGGATGATTCCGCGCTCCTGCTCGACCTCCGCCTCCTCGTTCTACAACTCGGGAGGTGGACTCGGGTCGCGATCCATGACGCCGGGTAGCGGCCGCTCCGATTCAATGCAGTACGCTTCCGGGGGTTACAGTGCTCGCTCGCCGGTCGGGTTCGCGTCCGAGGAGCTTTTGGCGGAGATGCTCGAGGCGCCCAAGGGTGGGGATAGCATATCGGTGACGATTCGGTTTCGGCCGCTGAGCGAGAGGGAGTTTCAGAGAGGGGATGAGGTCACTTGGTATGCAGACGGCGATAAGATTGTGAGGAATGAGTATAATCCAGCTACGGCCTATGCATTTGATAGAGTTTTTGGACAGCATGCCAATTCGCAAGAGGTGTACGAAGTTGCCGCTAAACCTGTTGTCAAAGCTGCCATGGAAGGTGTCAATGGTACCGTTTTTGCTTATGGTGTGACAAGTAGTGGAAAGACACACACTATGCATGGTGATCAAAATGCTCCAGGTGTCATACCGTTGGCGATAAAGGATGTCTTCAGTATTATTCAAGATACCCCAGGAAGGGAATTTTTACTGCGTGTCTCATACCTAGAGATCTACAACGAGGTGATAAATGACTTGCTTGATCCAACAGGTCAAAATTTGCGTGTTAGAGAAGATTCCCAGGGTACTTATGTTGAGGGTATAAAAGAAGAAGTCGTTTTGTCTCCTGGGCATGCACTTTCTTTCATTGCTGCTGGAGAAGAGCATCGTCATGTTGGATCAAACAATTTTAATCTGTTCAGCAGTCGAAGTCACACCATATTTACACTGATGATAGAAAGCAGTGCCCATGGTGATGAGTATGACGGAGTGATCTTCTCTCAACTTAATTTAATTGATCGAGCTGGATCTGAGAGCTCGAAAACCGAAACAACTGGACTAAGAAGAAAGGAAGGATCATACATAAACAAGAGTCTTCTAACTCTTGGAACTGTAATCGGAAAGCTGAGTGAGGGAAAGGCATCCCATGTGCCATATCGAGATTCTAAGCTTACCCGTCTTTTACAATCTTCGTTAAGCGGGCATGGACATGTTTCACTTATTTGCACGGTGACTCCTGCATCAAGTAGCATGGAGGAAACTCATAATACATTGAAATTTGCAAGCAGGGCAAAGCGAGTAGAAATCTATGCATCGCGTAATAAGCAGATTATTGATGAAAAGTCATTGATTAAGAAGTATCAAAGAGAGATTTCGGTCCTGAAAACAAACCTTGATCAACTAAGGCAGGGGATGCTTGTTGGCATCAGTCATGAGGAGATTGTCAGCTTAAAGCAAAAGTTGGAAGAAGGTCAATTTAAGATGCAATCAAGATTGGAGGAAGAAGAGGAAGCGAAGGCTGCTCTTATGAGTAGAATCCAGAGGCTGACGAAGCTCATCCTTGTTTCTTCGAAAAATACCATCCCTGGATTGAGTGATATTCCCAGCCACCAACGCGGTTTTTCTGTTGGTGAGGATGAAAAAATGGAAGTAATGCGAGATGGACCCTTGCTTCTAGAAGGTGAGAACCAAAAGGAGTCACCGTCTTCCGCATCTGCTGTTCCATCAGATATGGCTTATGATTTTAGACACAAGAGATCTTTGAGCAGGTGGAATGAAGAGCTCCCACCAGCTGGCAGTACAATTACAGATTCAACTCAAGCGGGTGAACTTATCAGTGGTTCAGGAGTTCCAATGGGAGGGATGACAATGTCAGATCACATGGACCTGCTGGTTGAGCAAGTTAAGATGCTTGCTGGAGAGATTGCACTGGGTACCAGCTCCTTGAAACGATTGGTGGAGCAGTCTGTAGATGACCCTGAAAGCTCCAAAACTCAAATTGAGAACTTGGAATGTGATATCCATGAAAAGAGAAGGCAAATGAGGGTTTTGGAACAGTGCATTAATGAAAGTGGTGAGGCTTCAATTGCTAATGCATCTATGGTTGAGATGCAGCAGACAGTTAAGAGACTGACAACCCAGTGCAATGAGAAGGGATTTGAGTTGGAAATAAAATCAGCAGACAATCGTATTCTCCAGGAGCAATTGCAAAATAAGTGCAGTGTGCAGAGAACATGGAATTGCAAGAAAAAGTGGATCGATTTAGAGCAGCGTTTGGCTTCGGTATCTGGTGAAAGATCATCACTGTCTTCCGAGCAGTGTGTATCTGAAGAGTATGTTGAGGAGTTGCAAAAGAAAATTCAGTCTCAGGGGATTGAGAATGAAAAACTAAAGCTGGAGCACGTGCAGTTCTCAGAGGAGAGGAGTGGGTTACATGTGCAGAACCAAAAACTGGCCGAAGAAGCTTCTTATGCAAAGGAACTGGCATCTGCTGCTGCTGTTGAATTGAAGAATTTGGCTGGTGAAGTGACAAAGCTCTCATTGCAGAATGCAAAACTAGAAAAAGAGTTGTTGGCTGCCCGGGAGTTAGTCAATTCTAAAAGTTCTTCCTTGCAACCTGTTAATGGCATTAATCGGAAGTACAATGATAGACCAAGAGGTGGGAGGAAAGGGAGGCTATCTGGTGGTGCTGATAACTTTGAGGCATGGAATCTTGATTCGGATGATTTAAGGATGGAACTCCAAGCAAGGAAACAGCGAGAGGCAGCTCTTGAGACTGCCCTTGCTGAAAAGGAATTTACAGAAGAGGAGTACAGGAAAAAGGTTGAAGATGCAAAGAAAAGGGAGGACGCTCTAGAAAATGATTTAGCAAACATGTGGGTGTTGGTCGCAAAATTAAAGAAAGAGGGCGGGTCTATCCCTGAGACACACACAGAAGAAAGGCATAATGATGTGATAGAAAATAGTAATGGTCTAAAAGCAACTGTGAATGAGATTGCCACCATAGAGAGACAAGTTTTGGATGCTTCAAAATCCGCTGATGATGAAAGTCCTAAGGAAGAACCTCTTGTTCTTCGCCTTAAGGCTCGAATGCAAGAGATGAAGGAGAAAGAGCTCAAACACCAGGGAAATGGAGATGCCAATTCCCATTTGTGTAAAGTATGTTTTGAATCACCAACAGCAGCAATTCTTCTCCCTTGCCGGCATTTTTGTTTGTGTAAGTCTTGTTCACTTGCATGTTCTGAGTGCCCAATTTGTCGTACAAAGATTTCAGATAGGCTTTTTGCATTTACTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031347 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0065007 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin zf-C3HC4_3

Protein Analysis

1086

Amino Acids

120.14

Weight (kDa)

5.92

Isoelectric Point (pI)

51.93

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21380 AT2G21380 AT4G39050
fragaria_vesca	FvH4_2g10080
malus_domestica	MD05G1058100.v1.1 MD10G1065900.v1.1
prunus_persica	Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1
pyrus_communis	pycom05g04920 pycom10g05130 pycom10g05140
rosa_chinensis	RchiOBHm_Chr6g0267011
rosa_laevigata	RLG00000014051 RLG00000027737 RLG00000037008
rosa_multiflora	Rmu_co8233357.1_g000001 Rmu_sc0013804.1_g000010 Rmu_sc0017071.1_g000008 Rmu_sc0037796.1_g000001 Rmu_sc0041080.1_g000001 Rmu_ssc0000341.1_g000028
rosa_roxburghii	Rroxscaffold_2G00112220 Rroxscaffold_7G00201230
rosa_rugosa	Rorug06G0028400 Rorug06G0028400 Rorug06G0028400
rosa_samantha	Rh1AG191400 Rh1CG176500 Rh6AG150000 Rh6CG146100
rosa_wichuraiana	Rw6G012990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1716
Acc36I	ACCTGC	2 cut(s)	1716, 1854
Acc65I	GGTACC	2 cut(s)	530, 1892
AccB1I	GGYRCC	3 cut(s)	289, 530, 1892
AccB7I	CCANNNNNTGG	3 cut(s)	1751, 2410, 2618
AccBSI	CCGCTC	1 cut(s)	198
AccII	CGCG	6 cut(s)	89, 115, 172, 257, 1276, 1575
AclWI	GGATC	6 cut(s)	168, 716, 865, 994, 1042, 2222
AcoI	YGGCCR	3 cut(s)	193, 332, 2412
AcsI	RAATTY	6 cut(s)	662, 736, 1235, 2316, 2470, 2753
AcuI	CTGAAG	2 cut(s)	616, 2307
AcvI	CACGTG	1 cut(s)	2362
AcyI	GRCGYC	2 cut(s)	182, 290
AfaI	GTAC	9 cut(s)	215, 479, 532, 766, 1759, 1894, 2600, 2771, 3220
AflII	CTTAAG	1 cut(s)	3038
AflIII	ACRYGT	3 cut(s)	1168, 2392, 2832
AjnI	CCWGG	7 cut(s)	598, 652, 759, 805, 1543, 2154, 3081
Alw21I	GWGCWC	5 cut(s)	238, 998, 1743, 2361, 3075
Alw26I	GTCTC	7 cut(s)	682, 807, 2080, 2328, 2726, 2876, 2962
AlwI	GGATC	6 cut(s)	168, 716, 865, 994, 1042, 2222
AlwNI	CAGNNNCTG	1 cut(s)	1504
Ama87I	CYCGRG	4 cut(s)	151, 164, 284, 2538
AoxI	GGCC	5 cut(s)	3, 193, 332, 426, 2412
ApoI	RAATTY	6 cut(s)	662, 736, 1235, 2316, 2470, 2753
AseI	ATTAAT	2 cut(s)	2022, 2589
Asp718I	GGTACC	2 cut(s)	530, 1892
AspLEI	GCGC	2 cut(s)	117, 292
AspS9I	GGNCC	3 cut(s)	1336, 1622, 1843
AsuC2I	CCSGG	4 cut(s)	186, 223, 2539, 2540
AsuII	TTCGAA	1 cut(s)	1529
AvaI	CYCGRG	4 cut(s)	151, 164, 284, 2538
AvaII	GGWCC	3 cut(s)	1336, 1622, 1843
AxyI	CCTNAGG	1 cut(s)	3012
BaeGI	GKGCMC	2 cut(s)	928, 3209
BaeI	ACNNNNGTAYC	2 cut(s)	756, 789
BanI	GGYRCC	3 cut(s)	289, 530, 1892
BanII	GRGCYC	4 cut(s)	22, 998, 1743, 3075
BauI	CACGAG	1 cut(s)	15
BbrPI	CACGTG	1 cut(s)	2362
BbsI	GAAGAC	4 cut(s)	622, 1046, 1656, 2259
Bbv12I	GWGCWC	5 cut(s)	238, 998, 1743, 2361, 3075
BccI	CCATC	5 cut(s)	61, 1547, 1613, 1690, 2686
BceAI	ACGGC	2 cut(s)	403, 441
BcgI	CGANNNNNNTGC	1 cut(s)	3234
BciT130I	CCWGG	7 cut(s)	600, 654, 761, 807, 1545, 2156, 3083
BclI	TGATCA	3 cut(s)	93, 586, 1354
BcnI	CCSGG	4 cut(s)	186, 223, 2539, 2540
BcoDI	GTCTC	7 cut(s)	682, 807, 2080, 2328, 2726, 2876, 2962
BfaI	CTAG	4 cut(s)	686, 1634, 2516, 2813
BfmI	CTRYAG	1 cut(s)	1926
BfoI	RGCGCY	1 cut(s)	293
BfrI	CTTAAG	1 cut(s)	3038
BfuAI	ACCTGC	2 cut(s)	1716, 1854
BglII	AGATCT	2 cut(s)	690, 1715
BlpI	GCTNAGC	1 cut(s)	338
Bme18I	GGWCC	3 cut(s)	1336, 1622, 1843
BmeT110I	CYCGRG	4 cut(s)	151, 164, 284, 2538
BmgT120I	GGNCC	3 cut(s)	1336, 1622, 1843
BmiI	GGNNCC	4 cut(s)	291, 532, 1624, 1894
BmrI	ACTGGG	2 cut(s)	1898, 2092
BmuI	ACTGGG	2 cut(s)	1898, 2092
BoxI	GACNNNNGTC	1 cut(s)	166
BpiI	GAAGAC	4 cut(s)	622, 1046, 1656, 2259
BplI	GAGNNNNNCTC	2 cut(s)	147, 179
BpmI	CTGGAG	5 cut(s)	582, 855, 1896, 2138, 2375
Bpu1102I	GCTNAGC	1 cut(s)	338
Bpu14I	TTCGAA	1 cut(s)	1529
BpuEI	CTTGAG	2 cut(s)	1761, 2750
BpuMI	CCSGG	4 cut(s)	186, 223, 2539, 2540
Bsa29I	ATCGAT	1 cut(s)	2217
BsaAI	YACGTR	1 cut(s)	2362
BsaHI	GRCGYC	2 cut(s)	182, 290
BsaWI	WCCGGW	1 cut(s)	45
BsaXI	ACNNNNNCTCC	4 cut(s)	1177, 1207, 1646, 1676
Bse118I	RCCGGY	2 cut(s)	244, 3159
Bse1I	ACTGG	6 cut(s)	57, 1018, 1893, 2098, 2415, 2445
Bse21I	CCTNAGG	1 cut(s)	3012
Bse3DI	GCAATG	3 cut(s)	825, 2110, 2498
BseBI	CCWGG	7 cut(s)	600, 654, 761, 807, 1545, 2156, 3083
BseCI	ATCGAT	1 cut(s)	2217
BseMI	GCAATG	3 cut(s)	825, 2110, 2498
BseMII	CTCAG	7 cut(s)	329, 981, 1076, 2339, 2385, 2871, 3192
BseNI	ACTGG	6 cut(s)	57, 1018, 1893, 2098, 2415, 2445
BseRI	GAGGAG	9 cut(s)	58, 67, 127, 278, 1409, 2315, 2390, 2395, 2780
BseSI	GKGCMC	2 cut(s)	928, 3209
BseX3I	CGGCCG	2 cut(s)	193, 332
BseYI	CCCAGC	1 cut(s)	1562
BsgI	GTGCAG	4 cut(s)	2197, 2204, 2384, 2417
Bsh1236I	CGCG	6 cut(s)	89, 115, 172, 257, 1276, 1575
Bsh1285I	CGRYCG	4 cut(s)	103, 196, 249, 335
BshFI	GGCC	5 cut(s)	5, 195, 334, 428, 2414
BshNI	GGYRCC	3 cut(s)	289, 530, 1892
BshVI	ATCGAT	1 cut(s)	2217
BsiEI	CGRYCG	4 cut(s)	103, 196, 249, 335
BsiHKAI	GWGCWC	5 cut(s)	238, 998, 1743, 2361, 3075
BsiHKCI	CYCGRG	4 cut(s)	151, 164, 284, 2538
BsiSI	CCGG	6 cut(s)	46, 185, 222, 245, 2539, 3160
BslFI	GGGAC	1 cut(s)	45
BsmAI	GTCTC	7 cut(s)	682, 807, 2080, 2328, 2726, 2876, 2962
BsmFI	GGGAC	1 cut(s)	45
BsmI	GAATGC	2 cut(s)	2512, 3054
BsnI	GGCC	5 cut(s)	5, 195, 334, 428, 2414
BsoBI	CYCGRG	4 cut(s)	151, 164, 284, 2538
Bsp119I	TTCGAA	1 cut(s)	1529
Bsp1286I	GDGCHC	8 cut(s)	22, 238, 928, 998, 1743, 2361, 3075, 3209
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	2 cut(s)	513, 928
Bsp68I	TCGCGA	1 cut(s)	172
BspANI	GGCC	5 cut(s)	5, 195, 334, 428, 2414
BspCNI	CTCAG	7 cut(s)	330, 982, 1077, 2338, 2384, 2872, 3193
BspDI	ATCGAT	1 cut(s)	2217
BspFNI	CGCG	6 cut(s)	89, 115, 172, 257, 1276, 1575
BspHI	TCATGA	1 cut(s)	1390
BspLI	GGNNCC	4 cut(s)	291, 532, 1624, 1894
BspMI	ACCTGC	2 cut(s)	1716, 1854
BspPI	GGATC	6 cut(s)	168, 716, 865, 994, 1042, 2222
BspQI	GCTCTTC	2 cut(s)	834, 1731
BspT104I	TTCGAA	1 cut(s)	1529
BspT107I	GGYRCC	3 cut(s)	289, 530, 1892
BspTI	CTTAAG	1 cut(s)	3038
BsrBI	CCGCTC	1 cut(s)	198
BsrDI	GCAATG	3 cut(s)	825, 2110, 2498
BsrFI	RCCGGY	2 cut(s)	244, 3159
BsrI	ACTGG	6 cut(s)	57, 1018, 1893, 2098, 2415, 2445
BssAI	RCCGGY	2 cut(s)	244, 3159
BssNI	GRCGYC	2 cut(s)	182, 290
BssSI	CACGAG	1 cut(s)	15
BssT1I	CCWWGG	3 cut(s)	294, 513, 928
Bst2BI	CACGAG	1 cut(s)	15
Bst2UI	CCWGG	7 cut(s)	600, 654, 761, 807, 1545, 2156, 3083
Bst6I	CTCTTC	5 cut(s)	834, 1458, 1731, 2283, 2757
BstACI	GRCGYC	2 cut(s)	182, 290
BstAFI	CTTAAG	1 cut(s)	3038
BstAPI	GCANNNNNTGC	2 cut(s)	1372, 2450
BstBAI	YACGTR	1 cut(s)	2362
BstBI	TTCGAA	1 cut(s)	1529
BstDSI	CCRYGG	2 cut(s)	513, 928
BstFNI	CGCG	6 cut(s)	89, 115, 172, 257, 1276, 1575
BstH2I	RGCGCY	1 cut(s)	293
BstHHI	GCGC	2 cut(s)	117, 292
BstMAI	GTCTC	7 cut(s)	682, 807, 2080, 2328, 2726, 2876, 2962
BstMCI	CGRYCG	4 cut(s)	103, 196, 249, 335
BstNI	CCWGG	7 cut(s)	600, 654, 761, 807, 1545, 2156, 3083
BstNSI	RCATGY	6 cut(s)	461, 815, 1172, 2396, 2836, 3198
BstPAI	GACNNNNGTC	1 cut(s)	166
BstSFI	CTRYAG	1 cut(s)	1926
BstSLI	GKGCMC	2 cut(s)	928, 3209
BstUI	CGCG	6 cut(s)	89, 115, 172, 257, 1276, 1575
BstV2I	GAAGAC	4 cut(s)	622, 1046, 1656, 2259
BstX2I	RGATCY	3 cut(s)	690, 986, 1715
BstXI	CCANNNNNNTGG	1 cut(s)	3089
BstYI	RGATCY	3 cut(s)	690, 986, 1715
BstZI	CGGCCG	2 cut(s)	193, 332
Bsu15I	ATCGAT	1 cut(s)	2217
Bsu36I	CCTNAGG	1 cut(s)	3012
BsuRI	GGCC	5 cut(s)	5, 195, 334, 428, 2414
BsuTUI	ATCGAT	1 cut(s)	2217
BtgI	CCRYGG	2 cut(s)	513, 928
BtgZI	GCGATG	1 cut(s)	1257
BtsI	GCAGTG	3 cut(s)	928, 2185, 2282
BtuMI	TCGCGA	1 cut(s)	172
BveI	ACCTGC	2 cut(s)	1716, 1854
CaiI	CAGNNNCTG	1 cut(s)	1504
CciI	TCATGA	1 cut(s)	1390
CciNI	GCGGCCGC	1 cut(s)	193
CfoI	GCGC	2 cut(s)	117, 292
Cfr10I	RCCGGY	2 cut(s)	244, 3159
Cfr13I	GGNCC	3 cut(s)	1336, 1622, 1843
Cfr9I	CCCGGG	1 cut(s)	2538
ClaI	ATCGAT	1 cut(s)	2217
CseI	GACGC	3 cut(s)	190, 246, 2816
Csp6I	GTAC	9 cut(s)	214, 478, 531, 765, 1758, 1893, 2599, 2770, 3219
CviQI	GTAC	9 cut(s)	214, 478, 531, 765, 1758, 1893, 2599, 2770, 3219
DinI	GGCGCC	1 cut(s)	291
EaeI	YGGCCR	3 cut(s)	193, 332, 2412
EagI	CGGCCG	2 cut(s)	193, 332
Eam1104I	CTCTTC	5 cut(s)	834, 1458, 1731, 2283, 2757
EarI	CTCTTC	5 cut(s)	834, 1458, 1731, 2283, 2757
EciI	GGCGGA	2 cut(s)	121, 290
Ecl136II	GAGCTC	3 cut(s)	996, 1741, 3073
EclXI	CGGCCG	2 cut(s)	193, 332
Eco130I	CCWWGG	3 cut(s)	294, 513, 928
Eco24I	GRGCYC	4 cut(s)	22, 998, 1743, 3075
Eco32I	GATATC	1 cut(s)	1978
Eco47I	GGWCC	3 cut(s)	1336, 1622, 1843
Eco52I	CGGCCG	2 cut(s)	193, 332
Eco53kI	GAGCTC	3 cut(s)	996, 1741, 3073
Eco57I	CTGAAG	2 cut(s)	616, 2307
Eco72I	CACGTG	1 cut(s)	2362
Eco81I	CCTNAGG	1 cut(s)	3012
Eco88I	CYCGRG	4 cut(s)	151, 164, 284, 2538
EcoICRI	GAGCTC	3 cut(s)	996, 1741, 3073
EcoRII	CCWGG	7 cut(s)	598, 652, 759, 805, 1543, 2154, 3081
EcoRV	GATATC	1 cut(s)	1978
EcoT14I	CCWWGG	3 cut(s)	294, 513, 928
EcoT22I	ATGCAT	4 cut(s)	436, 582, 1273, 2056
EcoT38I	GRGCYC	4 cut(s)	22, 998, 1743, 3075
EgeI	GGCGCC	1 cut(s)	291
EheI	GGCGCC	1 cut(s)	291
ErhI	CCWWGG	3 cut(s)	294, 513, 928
FalI	AAGNNNNNCTT	4 cut(s)	614, 646, 3009, 3041
FaqI	GGGAC	1 cut(s)	45
FauI	CCCGC	4 cut(s)	29, 1151, 1774, 2861
FbaI	TGATCA	3 cut(s)	93, 586, 1354
FriOI	GRGCYC	4 cut(s)	22, 998, 1743, 3075
FspBI	CTAG	4 cut(s)	686, 1634, 2516, 2813
GlaI	GCGC	2 cut(s)	116, 291
GsaI	CCCAGC	1 cut(s)	1566
GsuI	CTGGAG	5 cut(s)	582, 855, 1896, 2138, 2375
HaeII	RGCGCY	1 cut(s)	293
HaeIII	GGCC	5 cut(s)	5, 195, 334, 428, 2414
HapII	CCGG	6 cut(s)	46, 185, 222, 245, 2539, 3160
HgaI	GACGC	3 cut(s)	190, 246, 2816
HhaI	GCGC	2 cut(s)	117, 292
Hin1I	GRCGYC	2 cut(s)	182, 290
Hin6I	GCGC	2 cut(s)	115, 290
HinP1I	GCGC	2 cut(s)	115, 290
HindIII	AAGCTT	2 cut(s)	1127, 2421
HpaII	CCGG	6 cut(s)	46, 185, 222, 245, 2539, 3160
Hpy166II	GTNNAC	4 cut(s)	161, 478, 1787, 3188
Hpy8I	GTNNAC	4 cut(s)	161, 478, 1787, 3188
HpyAV	CCTTC	9 cut(s)	512, 651, 1025, 1421, 1468, 1631, 1986, 2104, 3055
HpyCH4IV	ACGT	1 cut(s)	2361
HpySE526I	ACGT	1 cut(s)	2361
Hsp92I	GRCGYC	2 cut(s)	182, 290
HspAI	GCGC	2 cut(s)	115, 290
KasI	GGCGCC	1 cut(s)	289
KpnI	GGTACC	2 cut(s)	534, 1896
KroI	GCCGGC	1 cut(s)	3159
KroNI	GCCGGC	1 cut(s)	3161
Ksp22I	TGATCA	3 cut(s)	93, 586, 1354
LguI	GCTCTTC	2 cut(s)	834, 1731
MaeI	CTAG	4 cut(s)	686, 1634, 2516, 2813
MaeII	ACGT	1 cut(s)	2361
MaeIII	GTNAC	9 cut(s)	227, 316, 370, 550, 890, 1189, 1656, 2388, 2485
MbiI	CCGCTC	1 cut(s)	198
MfeI	CAATTG	2 cut(s)	2043, 2162
MflI	RGATCY	3 cut(s)	690, 986, 1715
MhlI	GDGCHC	8 cut(s)	22, 238, 928, 998, 1743, 2361, 3075, 3209
Mly113I	GGCGCC	1 cut(s)	290
MlyI	GAGTC	4 cut(s)	156, 1060, 1186, 1664
MmeI	TCCRAC	5 cut(s)	83, 749, 835, 1401, 2103
Mph1103I	ATGCAT	4 cut(s)	436, 582, 1273, 2056
MroNI	GCCGGC	1 cut(s)	3159
MslI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2837, 2907, 3193
MspA1I	CMGCKG	3 cut(s)	337, 1751, 2996
MspCI	CTTAAG	1 cut(s)	3038
MspI	CCGG	6 cut(s)	46, 185, 222, 245, 2539, 3160
MunI	CAATTG	2 cut(s)	2043, 2162
Mva1269I	GAATGC	2 cut(s)	2512, 3054
MvaI	CCWGG	7 cut(s)	600, 654, 761, 807, 1545, 2156, 3083
MvnI	CGCG	6 cut(s)	89, 115, 172, 257, 1276, 1575
NaeI	GCCGGC	1 cut(s)	3161
NarI	GGCGCC	1 cut(s)	290
NciI	CCSGG	4 cut(s)	186, 223, 2539, 2540
NcoI	CCATGG	2 cut(s)	513, 928
NgoMIV	GCCGGC	1 cut(s)	3159
NlaIV	GGNNCC	4 cut(s)	291, 532, 1624, 1894
NmuCI	GTSAC	7 cut(s)	316, 370, 550, 890, 1189, 1656, 2485
NotI	GCGGCCGC	1 cut(s)	193
NruI	TCGCGA	1 cut(s)	172
NsiI	ATGCAT	4 cut(s)	436, 582, 1273, 2056
NspI	RCATGY	6 cut(s)	461, 815, 1172, 2396, 2836, 3198
NspV	TTCGAA	1 cut(s)	1529
PaeI	GCATGC	2 cut(s)	461, 815
PaeR7I	CTCGAG	1 cut(s)	284
PagI	TCATGA	1 cut(s)	1390
PaqCI	CACCTGC	1 cut(s)	1716
PasI	CCCWGGG	1 cut(s)	760
PciI	ACATGT	3 cut(s)	1168, 2392, 2832
PciSI	GCTCTTC	2 cut(s)	834, 1731
PcsI	WCGNNNNNNNCGW	1 cut(s)	86
PctI	GAATGC	2 cut(s)	2512, 3054
PdiI	GCCGGC	1 cut(s)	3161
PflFI	GACNNNGTC	1 cut(s)	1828
PflMI	CCANNNNNTGG	3 cut(s)	1751, 2410, 2618
PfoI	TCCNGGA	1 cut(s)	2154
PleI	GAGTC	4 cut(s)	156, 1059, 1186, 1663
PluTI	GGCGCC	1 cut(s)	293
PmaCI	CACGTG	1 cut(s)	2362
PmlI	CACGTG	1 cut(s)	2362
PpsI	GAGTC	4 cut(s)	156, 1059, 1186, 1663
Ppu21I	YACGTR	1 cut(s)	2362
PscI	ACATGT	3 cut(s)	1168, 2392, 2832
PshAI	GACNNNNGTC	1 cut(s)	166
PshBI	ATTAAT	2 cut(s)	2022, 2589
Psp124BI	GAGCTC	3 cut(s)	998, 1743, 3075
Psp6I	CCWGG	7 cut(s)	598, 652, 759, 805, 1543, 2154, 3081
PspCI	CACGTG	1 cut(s)	2362
PspFI	CCCAGC	1 cut(s)	1562
PspGI	CCWGG	7 cut(s)	598, 652, 759, 805, 1543, 2154, 3081
PspN4I	GGNNCC	4 cut(s)	291, 532, 1624, 1894
PspPI	GGNCC	3 cut(s)	1336, 1622, 1843
PspXI	VCTCGAGB	1 cut(s)	284
PsrI	GAACNNNNNNTAC	2 cut(s)	3169, 3201
PstNI	CAGNNNCTG	1 cut(s)	1504
PsuI	RGATCY	3 cut(s)	690, 986, 1715
PsyI	GACNNNGTC	1 cut(s)	1828
PvuII	CAGCTG	1 cut(s)	1751
RruI	TCGCGA	1 cut(s)	172
RsaI	GTAC	9 cut(s)	215, 479, 532, 766, 1759, 1894, 2600, 2771, 3220
RsaNI	GTAC	9 cut(s)	214, 478, 531, 765, 1758, 1893, 2599, 2770, 3219
RseI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2837, 2907, 3193
SacI	GAGCTC	3 cut(s)	998, 1743, 3075
SapI	GCTCTTC	2 cut(s)	834, 1731
Sau96I	GGNCC	3 cut(s)	1336, 1622, 1843
SchI	GAGTC	4 cut(s)	156, 1060, 1186, 1664
SduI	GDGCHC	8 cut(s)	22, 238, 928, 998, 1743, 2361, 3075, 3209
SfcI	CTRYAG	1 cut(s)	1926
SfoI	GGCGCC	1 cut(s)	291
Sfr274I	CTCGAG	1 cut(s)	284
SfuI	TTCGAA	1 cut(s)	1529
SinI	GGWCC	3 cut(s)	1336, 1622, 1843
SlaI	CTCGAG	1 cut(s)	284
SmaI	CCCGGG	1 cut(s)	2540
SmiMI	CAYNNNNRTG	7 cut(s)	314, 849, 933, 1167, 2837, 2907, 3193
SmlI	CTYRAG	4 cut(s)	284, 1776, 2729, 3038
SmoI	CTYRAG	4 cut(s)	284, 1776, 2729, 3038
SphI	GCATGC	2 cut(s)	461, 815
SspDI	GGCGCC	1 cut(s)	289
SspMI	CTAG	4 cut(s)	686, 1634, 2516, 2813
SstI	GAGCTC	3 cut(s)	998, 1743, 3075
StyI	CCWWGG	3 cut(s)	294, 513, 928
TaiI	ACGT	1 cut(s)	2364
TaqI	TCGA	9 cut(s)	125, 285, 886, 979, 998, 1117, 1529, 2217, 3046
TaqII	GACCGA	1 cut(s)	1324
TatI	WGTACW	3 cut(s)	1757, 2598, 2769
TauI	GCSGC	5 cut(s)	89, 195, 198, 337, 491
TseFI	GTSAC	7 cut(s)	316, 370, 550, 890, 1189, 1656, 2485
Tsp45I	GTSAC	7 cut(s)	316, 370, 550, 890, 1189, 1656, 2485
TspDTI	ATGAA	9 cut(s)	814, 1311, 1611, 1749, 1998, 2040, 2355, 3018, 3074
TspGWI	ACGGA	1 cut(s)	960
TspMI	CCCGGG	1 cut(s)	2538
Tth111I	GACNNNGTC	1 cut(s)	1828
Van91I	CCANNNNNTGG	3 cut(s)	1751, 2410, 2618
Vha464I	CTTAAG	1 cut(s)	3038
VpaK11BI	GGWCC	3 cut(s)	1336, 1622, 1843
VspI	ATTAAT	2 cut(s)	2022, 2589
XapI	RAATTY	6 cut(s)	662, 736, 1235, 2316, 2470, 2753
XbaI	TCTAGA	2 cut(s)	1633, 2812
XceI	RCATGY	6 cut(s)	461, 815, 1172, 2396, 2836, 3198
XhoI	CTCGAG	1 cut(s)	284
XmaI	CCCGGG	1 cut(s)	2538
XspI	CTAG	4 cut(s)	686, 1634, 2516, 2813
Zsp2I	ATGCAT	4 cut(s)	436, 582, 1273, 2056

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.