Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004310

Physical Location & Seq

Genomic Coordinates Forward (+)

46207434 .. 46216658

Gene Structure 9225 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014051

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3255 bp

ATGGCCTCGTCCTCCAGAGCAAGGAGCAGCTCGCCATTCTCGTACCGCAAGCCTTCAAGTCCCTACTCCTCGACCTCCTCCTCGTCCTCGCTGATCAACGGCCGCGCTCTCATTCCGCGCTCCTGCTCCAGCTCAGCAACGTCGTTTTACAACTCCGGAAGCGGACTCGGTCCCCGATCCGCCACGCCGAGCCGCTCCCGCTCCGAATCAATGTACCAAGGCTCGCGGTCCTACGGCGGCGTCTCGCCGGTGGGGTTCGCTGCGGAGGACCTTATGACCGAGATTGTGGATGCGCCGAGGTCTGGGGATAGCATATCGGTCACGATTCGCTTTCGGCCTCTCAGCGAGAGGGAGTATCAGAGAGGGGATGAGGTCACTTGGTATGCAGATGGTGATAAGATTGTGCGGAATGCGTACAATCCAGTTACAGCCTATGCATTCGATAAAGTTTTTGGACAGGATACGAATTCTCAAGAGGTGTATGAGGTTGCTGCCAAACCTGTGGTCAAAGCTGCCATGGAAGGTGTCAATGGCACTGTTTTTGCCTATGGTGTGACAAGTAGTGGGAAGACGCACACCATGCATGGAGATCAAAACGCTCCAGGTGTTATACCATTGGCCATAAAAGATGTATTCAGTATGATCCAAGATACCCCAGGAAGGGAGTTTTTACTTCGTGTCTCGTATCTTGAGATCTACAATGAGGTGATAAATGACTTGCTTGACCCAACAGGTCAAAATTTGCGTGTTAGAGAAGATTCACAGGGTACATATGTTGAGGGTATAAAAGAAGAAGTTGTTCTGTCTCCTGGGCATGCACTTTCGTTTATTGCTGCTGGAGAAGAGCACCGTCATGTTGGATCAAACAATTATAATTTGTTCAGTAGTAGAAGTCACACCATATTTACACTGATGATTGAAAGTAGTGCCCATGGGGATGAATATGATGGAGTGATCTTCTCTCAACTTAACTTGATTGATCTAGCTGGATCGGAGAGCTCTAAAACCGAAACAACTGGACTAAGAAGAAAGGAAGGATCTTACATTAACAAGAGCCTTTTAACTCTTGGAACTGTAATTGGGAAGCTGAGTGAGGGAAAGGCATCCCATGTTCCATATCGAGATTCTAAGCTCACCCGCCTTTTACAATCTTCGCTAAGTGGGCATGGACATGTTTCGCTCATTTGCACGGTTACTCCTGCATCAAGTAGCATGGAGGAAACCCATAATACATTGAAGTTCGCAAGCAGAGCAAAGCGAGTAGAGATCTATGCATCACGTAATAAGATTATTGATGAAAAGTCTTTAATTAAGAAGTATCAAAGAGAAATTTCAGTCCTTAAAGAAGAGCTTGATCAGCTAAGGAAGGGCATGCTTGTTGGCATCAGTCATGAGGAAATTATCACTTTAAAGCAGAAGTTGGAAGAAGGCCAAGTCAAAATGCAATCAAGATTGGAGGAAGAAGAGGAAGCAAAGGCTGCTCTTATGAGTAGAATCCAGAGGCTGACGAAGCTCATTCTTGTTTCATCAAAAAATACCATACCTGGATGTTTGAGTGATATTCCCACCCACCAACGACGTTATTCTGTTGGTGAGGACGATAAAACAGAAGTAGTGCGAGACGGGTCCTTGCTAGTAGAAAGTGAGAACCAGGACTCACCTTCTTCTGCCTCTGGTGTCCCATCAGATCTGTCTTATGATATTAGACACAAGAGATCTTCCAGCAGGTGGAATGAAGAGTTCTCACCAGCTGGCAGTACAATTACAGAATCAACTCAAGCTGGGGAACTTATCAGTGGTTCAAAACTGACAACAGGAGGGATGACAATGACAGATCATATAGACCTGCTTGTCGAGCAAGTAAAGATGCTTGCTGGAGAGATAGCACTTGGCACCAGCACCTTGAAGCGAATGGTGGAGCAGTCTGTAAATGACCCTGATAGCTCCAAAGTTCAAATTGAGAACTTGGAACGTGATATCCATGAAAAGAGAAGGCAAATGAGGGTTTTAGAACAGCGCATCAATGAAAGTGGCGAGGCTTCAGTCTCTAACGCATCTTTGGTTGAGATGCAGCAGACAGTTAAGAGACTGATGACACAGTGCGATGAGAAGGGGTTTGAGTTGGAAATAAAATCGGCAGACAATCGTATTCTTCAAGAGCAACTGCAAAATAAGTGTGCAGAGAACTTGGAATTGCAAGAAAAAGTGAATCAGTTAGAGCGACGCTTAGCTTCAGGGTTTTCCGAGCACGGTGTATCTGAAGAGTATGTTGATGAATTGAGAAAGAAAATCCAGTCTCAGGAGATTGAGAATGAAAGACTAAAATTGGAGCATGTCCAGTTCTCAGAGGAGAATAGTGGGTTACATGTGCAAAATCAGAAACTGGCTGAAGAAGCTTCTTATGCAAAGGAACTGGCATCTGCTGCCGCTGTTGAGTTGAAAAACTTGGCCGGTGAAGTGACAAAACTGTCATTACAGAGTGCTAAACTGGAGAAAGAGTTGTTGGCTGCTCGTGAGCTAGCCAATAATTCTAGAAGTTCTAACATGCATCCTGGTAATGGCGTCAACCGCAAGTACAATGATGGATCAAGACCTGGGAGGAAAGGGAGGCTTTCTGGTCGTGCTAATGATATGCTGTCTGAGGACTTTGAGTCTTGGAATCTTGATCCTGATGATTTAAGGATGGAACTCCAGGCTAGGAAACAGCGAGAGGCAGCTCTTGAGGCTGCTCTGTCTGAAAAGGAATTTGTAGAGGAGGAATACAGGAAAAAGGTTGAAGATGCAAAGAAAAGAGAAGAAGCTCTGGAAAATGACTTGGCAAACATGTGGGTTCTGGTTGCAAAATTAAAGAAAGAGGGTGGAGCTATCCCTGAGACAACCACAGAAGAAAGACACAACGATGCCATGGAAACCAATAATGGTTTTAAAGCAAATGACAATGAAAGTAATACTATCCCCAAAGAGAGACAAGCGTTGGATGTTTCAAAACCAGCTAATGATGAAGTTCGCACCGAAGAACCCCTCGTTTTTCGCCTTAAGGCTCGAATGCAAGAGATGAAGGAAAAAGAGCTTAAACACCAGGGAAATGGTGATGCCAATTCCCATCTCTGTAAAGTATGCTTTGAAGCACCAACTGCGGCAATTCTATTGCCTTGCCGACATTTTTGTTTATGTAAATCGTGTTCACTTGCTTGTTCCGAGTGCCCAATTTGTCGTACAAAGATTGCAGATAGGCTTTTTGCATTTACTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031347 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0065007 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin zf-C3HC4_3

Protein Analysis

1085

Amino Acids

120.43

Weight (kDa)

5.76

Isoelectric Point (pI)

53.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	110 - 421	2e-105	Kinesin motor domain
Microtub_bd	PF16796	134 - 241	2.3e-19	Microtubule binding
zf-C3HC4_3	PF13920	1034 - 1076	1.8e-11	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21380 AT2G21380 AT4G39050
fragaria_vesca	FvH4_2g10080
malus_domestica	MD05G1058100.v1.1 MD10G1065900.v1.1
prunus_persica	Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1 Prupe.8G087800_v2.0.a1
pyrus_communis	pycom05g04920 pycom10g05130 pycom10g05140
rosa_chinensis	RchiOBHm_Chr6g0267011
rosa_laevigata	RLG00000014051 RLG00000027737 RLG00000037008
rosa_multiflora	Rmu_co8233357.1_g000001 Rmu_sc0013804.1_g000010 Rmu_sc0017071.1_g000008 Rmu_sc0037796.1_g000001 Rmu_sc0041080.1_g000001 Rmu_ssc0000341.1_g000028
rosa_roxburghii	Rroxscaffold_2G00112220 Rroxscaffold_7G00201230
rosa_rugosa	Rorug06G0028400 Rorug06G0028400 Rorug06G0028400
rosa_samantha	Rh1AG191400 Rh1CG176500 Rh6AG150000 Rh6CG146100
rosa_wichuraiana	Rw6G012990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	873
AarI	CACCTGC	1 cut(s)	1716
AasI	GACNNNNNNGTC	2 cut(s)	1850, 2467
Acc36I	ACCTGC	2 cut(s)	1716, 1854
AccB1I	GGYRCC	1 cut(s)	1892
AccB7I	CCANNNNNTGG	1 cut(s)	1728
AccBSI	CCGCTC	2 cut(s)	195, 201
AccII	CGCG	3 cut(s)	105, 118, 226
AccIII	TCCGGA	1 cut(s)	155
AclWI	GGATC	7 cut(s)	171, 637, 868, 997, 1045, 2593, 2660
AcoI	YGGCCR	3 cut(s)	100, 618, 2448
AcsI	RAATTY	4 cut(s)	466, 739, 1329, 2744
AcuI	CTGAAG	4 cut(s)	2025, 2217, 2280, 2409
AcyI	GRCGYC	2 cut(s)	240, 2562
AfaI	GTAC	7 cut(s)	44, 215, 416, 769, 1759, 2576, 3217
AfiI	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1728, 2299, 2558
AflII	CTTAAG	1 cut(s)	3035
AflIII	ACRYGT	3 cut(s)	1171, 2365, 2823
AhdI	GACNNNNNGTC	1 cut(s)	2650
AjnI	CCWGG	9 cut(s)	601, 655, 808, 1543, 1650, 2551, 2593, 2691, 3078
AjuI	GAANNNNNNNTTGG	2 cut(s)	2486, 2518
AloI	GAACNNNNNNTCC	2 cut(s)	1962, 1994
Alw21I	GWGCWC	3 cut(s)	849, 1001, 2250
Alw26I	GTCTC	9 cut(s)	247, 685, 810, 1614, 2050, 2080, 2301, 2867, 2959
AlwI	GGATC	7 cut(s)	171, 637, 868, 997, 1045, 2593, 2660
AlwNI	CAGNNNCTG	2 cut(s)	1504, 2383
Aor13HI	TCCGGA	1 cut(s)	155
AoxI	GGCC	6 cut(s)	3, 100, 335, 618, 1429, 2448
ApoI	RAATTY	4 cut(s)	466, 739, 1329, 2744
ArsI	GACNNNNNNTTYG	2 cut(s)	489, 521
Asp700I	GAANNNNTTC	1 cut(s)	798
AspLEI	GCGC	4 cut(s)	107, 120, 295, 2019
AspS9I	GGNCC	4 cut(s)	170, 228, 268, 1626
AsuHPI	GGTGA	8 cut(s)	404, 718, 1126, 1604, 1650, 1737, 2465, 3101
AsuNHI	GCTAGC	1 cut(s)	2518
AvaII	GGWCC	4 cut(s)	170, 228, 268, 1626
BaeGI	GKGCMC	2 cut(s)	931, 3206
BaeI	ACNNNNGTAYC	2 cut(s)	759, 792
BalI	TGGCCA	1 cut(s)	620
BanI	GGYRCC	1 cut(s)	1892
BanII	GRGCYC	1 cut(s)	1001
BauI	CACGAG	1 cut(s)	2511
BbsI	GAAGAC	1 cut(s)	575
Bbv12I	GWGCWC	3 cut(s)	849, 1001, 2250
BccI	CCATC	6 cut(s)	383, 941, 1690, 2576, 2677, 3111
BceAI	ACGGC	2 cut(s)	115, 250
BcgI	CGANNNNNNTGC	1 cut(s)	3231
BciT130I	CCWGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
BciVI	GTATCC	1 cut(s)	454
BclI	TGATCA	2 cut(s)	93, 1354
BcoDI	GTCTC	9 cut(s)	247, 685, 810, 1614, 2050, 2080, 2301, 2867, 2959
BfaI	CTAG	5 cut(s)	983, 1634, 2519, 2532, 2697
BfrI	CTTAAG	1 cut(s)	3035
BfuAI	ACCTGC	2 cut(s)	1716, 1854
BfuI	GTATCC	1 cut(s)	454
BglII	AGATCT	4 cut(s)	693, 1266, 1687, 1715
BlpI	GCTNAGC	2 cut(s)	133, 2227
Bme1390I	CCNGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
Bme18I	GGWCC	4 cut(s)	170, 228, 268, 1626
BmeRI	GACNNNNNGTC	1 cut(s)	2650
BmgT120I	GGNCC	4 cut(s)	170, 228, 268, 1626
BmiI	GGNNCC	3 cut(s)	172, 1627, 1894
BmrFI	CCNGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
BmtI	GCTAGC	1 cut(s)	2522
BpiI	GAAGAC	1 cut(s)	575
BpmI	CTGGAG	6 cut(s)	112, 585, 858, 1896, 2510, 2675
Bpu10I	CCTNAGC	1 cut(s)	1361
Bpu1102I	GCTNAGC	2 cut(s)	133, 2227
BpuEI	CTTGAG	4 cut(s)	456, 710, 1761, 2741
BsaAI	YACGTR	1 cut(s)	1280
BsaHI	GRCGYC	2 cut(s)	240, 2562
BsaJI	CCNNGG	9 cut(s)	217, 296, 516, 655, 809, 931, 2594, 2904, 3079
BsaWI	WCCGGW	1 cut(s)	155
BsaXI	ACNNNNNCTCC	4 cut(s)	1180, 1210, 2483, 2513
Bsc4I	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1728, 2299, 2558
Bse118I	RCCGGY	2 cut(s)	247, 2450
Bse1I	ACTGG	7 cut(s)	422, 1021, 2293, 2338, 2388, 2418, 2493
BseAI	TCCGGA	1 cut(s)	155
BseBI	CCWGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
BseDI	CCNNGG	9 cut(s)	217, 296, 516, 655, 809, 931, 2594, 2904, 3079
BseGI	GGATG	9 cut(s)	295, 373, 943, 1103, 1553, 1827, 2548, 2688, 2983
BseLI	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1728, 2299, 2558
BseMII	CTCAG	7 cut(s)	147, 355, 1079, 2312, 2358, 2631, 2862
BseNI	ACTGG	7 cut(s)	422, 1021, 2293, 2338, 2388, 2418, 2493
BseRI	GAGGAG	5 cut(s)	58, 67, 70, 2363, 2768
BseSI	GKGCMC	2 cut(s)	931, 3206
BseX3I	CGGCCG	1 cut(s)	100
BseYI	CCCAGC	1 cut(s)	1781
BsgI	GTGCAG	1 cut(s)	2199
Bsh1236I	CGCG	3 cut(s)	105, 118, 226
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	6 cut(s)	5, 102, 337, 620, 1431, 2450
BshNI	GGYRCC	1 cut(s)	1892
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	3 cut(s)	849, 1001, 2250
BsiSI	CCGG	3 cut(s)	156, 248, 2451
BslFI	GGGAC	3 cut(s)	45, 156, 1664
BslI	CCNNNNNNNGG	7 cut(s)	21, 302, 660, 661, 1728, 2299, 2558
BsmAI	GTCTC	9 cut(s)	247, 685, 810, 1614, 2050, 2080, 2301, 2867, 2959
BsmBI	CGTCTC	2 cut(s)	247, 1614
BsmFI	GGGAC	3 cut(s)	45, 156, 1664
BsmI	GAATGC	3 cut(s)	415, 437, 3051
BsnI	GGCC	6 cut(s)	5, 102, 337, 620, 1431, 2450
Bsp1286I	GDGCHC	5 cut(s)	849, 931, 1001, 2250, 3206
Bsp13I	TCCGGA	1 cut(s)	155
Bsp1720I	GCTNAGC	2 cut(s)	133, 2227
Bsp19I	CCATGG	3 cut(s)	516, 931, 2904
BspANI	GGCC	6 cut(s)	5, 102, 337, 620, 1431, 2450
BspCNI	CTCAG	7 cut(s)	146, 354, 1080, 2311, 2357, 2632, 2863
BspEI	TCCGGA	1 cut(s)	155
BspFNI	CGCG	3 cut(s)	105, 118, 226
BspHI	TCATGA	1 cut(s)	1390
BspLI	GGNNCC	3 cut(s)	172, 1627, 1894
BspMI	ACCTGC	2 cut(s)	1716, 1854
BspOI	GCTAGC	1 cut(s)	2522
BspPI	GGATC	7 cut(s)	171, 637, 868, 997, 1045, 2593, 2660
BspQI	GCTCTTC	2 cut(s)	837, 1341
BspT107I	GGYRCC	1 cut(s)	1892
BspTI	CTTAAG	1 cut(s)	3035
BsrBI	CCGCTC	2 cut(s)	195, 201
BsrFI	RCCGGY	2 cut(s)	247, 2450
BsrI	ACTGG	7 cut(s)	422, 1021, 2293, 2338, 2388, 2418, 2493
BssAI	RCCGGY	2 cut(s)	247, 2450
BssECI	CCNNGG	9 cut(s)	217, 296, 516, 655, 809, 931, 2594, 2904, 3079
BssNI	GRCGYC	2 cut(s)	240, 2562
BssSI	CACGAG	1 cut(s)	2511
BssT1I	CCWWGG	4 cut(s)	217, 516, 931, 2904
Bst2BI	CACGAG	1 cut(s)	2511
Bst2UI	CCWGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
Bst4CI	ACNGT	8 cut(s)	538, 851, 1075, 1192, 2080, 2100, 2252, 2469
Bst6I	CTCTTC	5 cut(s)	837, 1341, 1458, 1731, 2256
BstACI	GRCGYC	2 cut(s)	240, 2562
BstAFI	CTTAAG	1 cut(s)	3035
BstAPI	GCANNNNNTGC	4 cut(s)	580, 1478, 2423, 3134
BstBAI	YACGTR	1 cut(s)	1280
BstC8I	GCNNGC	9 cut(s)	32, 50, 224, 816, 1246, 1373, 1753, 1872, 2520
BstDSI	CCRYGG	3 cut(s)	516, 931, 2904
BstF5I	GGATG	9 cut(s)	295, 373, 943, 1103, 1553, 1827, 2548, 2688, 2983
BstFNI	CGCG	3 cut(s)	105, 118, 226
BstHHI	GCGC	4 cut(s)	107, 120, 295, 2019
BstMAI	GTCTC	9 cut(s)	247, 685, 810, 1614, 2050, 2080, 2301, 2867, 2959
BstMCI	CGRYCG	1 cut(s)	103
BstNI	CCWGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
BstNSI	RCATGY	7 cut(s)	818, 1175, 1375, 2336, 2369, 2548, 2827
BstSCI	CCNGG	9 cut(s)	601, 655, 808, 1543, 1650, 2551, 2593, 2691, 3078
BstSLI	GKGCMC	2 cut(s)	931, 3206
BstUI	CGCG	3 cut(s)	105, 118, 226
BstV2I	GAAGAC	1 cut(s)	575
BstX2I	RGATCY	5 cut(s)	693, 1037, 1266, 1687, 1715
BstXI	CCANNNNNNTGG	2 cut(s)	502, 3086
BstYI	RGATCY	5 cut(s)	693, 1037, 1266, 1687, 1715
BstZI	CGGCCG	1 cut(s)	100
BsuI	GTATCC	1 cut(s)	454
BsuRI	GGCC	6 cut(s)	5, 102, 337, 620, 1431, 2450
BtgI	CCRYGG	3 cut(s)	516, 931, 2904
BtgZI	GCGATG	1 cut(s)	2118
BtsCI	GGATG	9 cut(s)	295, 373, 943, 1103, 1553, 1827, 2548, 2688, 2983
BtsIMutI	CAGTG	4 cut(s)	534, 908, 1801, 2105
BveI	ACCTGC	2 cut(s)	1716, 1854
Cac8I	GCNNGC	9 cut(s)	32, 50, 224, 816, 1246, 1373, 1753, 1872, 2520
CaiI	CAGNNNCTG	2 cut(s)	1504, 2383
CciI	TCATGA	1 cut(s)	1390
CfoI	GCGC	4 cut(s)	107, 120, 295, 2019
Cfr10I	RCCGGY	2 cut(s)	247, 2450
Cfr13I	GGNCC	4 cut(s)	170, 228, 268, 1626
CseI	GACGC	4 cut(s)	229, 580, 2232, 2551
Csp6I	GTAC	7 cut(s)	43, 214, 415, 768, 1758, 2575, 3216
CspCI	CAANNNNNGTGG	2 cut(s)	2011, 2046
CviQI	GTAC	7 cut(s)	43, 214, 415, 768, 1758, 2575, 3216
DraI	TTTAAA	2 cut(s)	1410, 2926
DrdI	GACNNNNNNGTC	2 cut(s)	1850, 2467
DriI	GACNNNNNGTC	1 cut(s)	2650
DseDI	GACNNNNNNGTC	2 cut(s)	1850, 2467
EaeI	YGGCCR	3 cut(s)	100, 618, 2448
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	5 cut(s)	837, 1341, 1458, 1731, 2256
Eam1105I	GACNNNNNGTC	1 cut(s)	2650
EarI	CTCTTC	5 cut(s)	837, 1341, 1458, 1731, 2256
EciI	GGCGGA	1 cut(s)	169
Ecl136II	GAGCTC	1 cut(s)	999
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	4 cut(s)	217, 516, 931, 2904
Eco24I	GRGCYC	1 cut(s)	1001
Eco32I	GATATC	1 cut(s)	1978
Eco47I	GGWCC	4 cut(s)	170, 228, 268, 1626
Eco52I	CGGCCG	1 cut(s)	100
Eco53kI	GAGCTC	1 cut(s)	999
Eco57I	CTGAAG	4 cut(s)	2025, 2217, 2280, 2409
EcoICRI	GAGCTC	1 cut(s)	999
EcoO109I	RGGNCCY	2 cut(s)	268, 1626
EcoRI	GAATTC	1 cut(s)	466
EcoRII	CCWGG	9 cut(s)	601, 655, 808, 1543, 1650, 2551, 2593, 2691, 3078
EcoRV	GATATC	1 cut(s)	1978
EcoT14I	CCWWGG	4 cut(s)	217, 516, 931, 2904
EcoT22I	ATGCAT	4 cut(s)	439, 585, 1276, 2550
EcoT38I	GRGCYC	1 cut(s)	1001
ErhI	CCWWGG	4 cut(s)	217, 516, 931, 2904
Esp3I	CGTCTC	2 cut(s)	247, 1614
FalI	AAGNNNNNCTT	4 cut(s)	1335, 1367, 2595, 2627
FaqI	GGGAC	3 cut(s)	45, 156, 1664
FauI	CCCGC	2 cut(s)	206, 1145
FauNDI	CATATG	1 cut(s)	772
FbaI	TGATCA	2 cut(s)	93, 1354
FokI	GGATG	9 cut(s)	302, 380, 950, 1090, 1560, 1834, 2535, 2695, 2990
FriOI	GRGCYC	1 cut(s)	1001
FspBI	CTAG	5 cut(s)	983, 1634, 2519, 2532, 2697
GlaI	GCGC	4 cut(s)	106, 119, 294, 2018
GsaI	CCCAGC	1 cut(s)	1785
GsuI	CTGGAG	6 cut(s)	112, 585, 858, 1896, 2510, 2675
HaeIII	GGCC	6 cut(s)	5, 102, 337, 620, 1431, 2450
HapII	CCGG	3 cut(s)	156, 248, 2451
HgaI	GACGC	4 cut(s)	229, 580, 2232, 2551
HhaI	GCGC	4 cut(s)	107, 120, 295, 2019
Hin1I	GRCGYC	2 cut(s)	240, 2562
Hin6I	GCGC	4 cut(s)	105, 118, 293, 2017
HinP1I	GCGC	4 cut(s)	105, 118, 293, 2017
HincII	GTYRAC	1 cut(s)	2566
HindII	GTYRAC	1 cut(s)	2566
HindIII	AAGCTT	1 cut(s)	2394
HpaII	CCGG	3 cut(s)	156, 248, 2451
HphI	GGTGA	8 cut(s)	404, 718, 1126, 1604, 1650, 1737, 2465, 3101
Hpy166II	GTNNAC	2 cut(s)	2566, 3185
Hpy8I	GTNNAC	2 cut(s)	2566, 3185
Hpy99I	CGWCG	3 cut(s)	145, 1581, 2226
HpyCH4III	ACNGT	8 cut(s)	538, 851, 1075, 1192, 2080, 2100, 2252, 2469
HpyCH4IV	ACGT	4 cut(s)	140, 1279, 1579, 1972
HpySE526I	ACGT	4 cut(s)	140, 1279, 1579, 1972
Hsp92I	GRCGYC	2 cut(s)	240, 2562
HspAI	GCGC	4 cut(s)	105, 118, 293, 2017
Kpn2I	TCCGGA	1 cut(s)	155
Ksp22I	TGATCA	2 cut(s)	93, 1354
LguI	GCTCTTC	2 cut(s)	837, 1341
MaeI	CTAG	5 cut(s)	983, 1634, 2519, 2532, 2697
MaeII	ACGT	4 cut(s)	140, 1279, 1579, 1972
MaeIII	GTNAC	8 cut(s)	319, 373, 424, 553, 893, 1192, 2361, 2458
MbiI	CCGCTC	2 cut(s)	195, 201
MflI	RGATCY	5 cut(s)	693, 1037, 1266, 1687, 1715
MhlI	GDGCHC	5 cut(s)	849, 931, 1001, 2250, 3206
MlsI	TGGCCA	1 cut(s)	620
MluNI	TGGCCA	1 cut(s)	620
MlyI	GAGTC	3 cut(s)	159, 1649, 2660
MmeI	TCCRAC	4 cut(s)	838, 1401, 2103, 2955
Mox20I	TGGCCA	1 cut(s)	620
Mph1103I	ATGCAT	4 cut(s)	439, 585, 1276, 2550
MroI	TCCGGA	1 cut(s)	155
MroXI	GAANNNNTTC	1 cut(s)	798
MscI	TGGCCA	1 cut(s)	620
MslI	CAYNNNNRTG	4 cut(s)	852, 936, 1170, 2898
Msp20I	TGGCCA	1 cut(s)	620
MspA1I	CMGCKG	2 cut(s)	1751, 2429
MspCI	CTTAAG	1 cut(s)	3035
MspI	CCGG	3 cut(s)	156, 248, 2451
MspR9I	CCNGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
Mva1269I	GAATGC	3 cut(s)	415, 437, 3051
MvaI	CCWGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
MvnI	CGCG	3 cut(s)	105, 118, 226
NcoI	CCATGG	3 cut(s)	516, 931, 2904
NdeI	CATATG	1 cut(s)	772
NheI	GCTAGC	1 cut(s)	2518
NlaIV	GGNNCC	3 cut(s)	172, 1627, 1894
NmeAIII	GCCGAG	2 cut(s)	213, 321
NmuCI	GTSAC	5 cut(s)	319, 373, 553, 893, 2458
NsiI	ATGCAT	4 cut(s)	439, 585, 1276, 2550
NspI	RCATGY	7 cut(s)	818, 1175, 1375, 2336, 2369, 2548, 2827
PacI	TTAATTAA	1 cut(s)	1311
PaeI	GCATGC	2 cut(s)	818, 1375
PagI	TCATGA	1 cut(s)	1390
PaqCI	CACCTGC	1 cut(s)	1716
PciI	ACATGT	3 cut(s)	1171, 2365, 2823
PciSI	GCTCTTC	2 cut(s)	837, 1341
PcsI	WCGNNNNNNNCGW	1 cut(s)	38
PctI	GAATGC	3 cut(s)	415, 437, 3051
PdmI	GAANNNNTTC	1 cut(s)	798
PfeI	GAWTC	8 cut(s)	206, 325, 758, 1124, 1494, 1769, 2209, 2659
PflFI	GACNNNGTC	1 cut(s)	168
PflMI	CCANNNNNTGG	1 cut(s)	1728
PleI	GAGTC	3 cut(s)	159, 1649, 2659
PpsI	GAGTC	3 cut(s)	159, 1649, 2659
Ppu21I	YACGTR	1 cut(s)	1280
PpuMI	RGGWCCY	2 cut(s)	268, 1626
PscI	ACATGT	3 cut(s)	1171, 2365, 2823
PsiI	TTATAA	1 cut(s)	873
Psp124BI	GAGCTC	1 cut(s)	1001
Psp5II	RGGWCCY	2 cut(s)	268, 1626
Psp6I	CCWGG	9 cut(s)	601, 655, 808, 1543, 1650, 2551, 2593, 2691, 3078
PspFI	CCCAGC	1 cut(s)	1781
PspGI	CCWGG	9 cut(s)	601, 655, 808, 1543, 1650, 2551, 2593, 2691, 3078
PspN4I	GGNNCC	3 cut(s)	172, 1627, 1894
PspPI	GGNCC	4 cut(s)	170, 228, 268, 1626
PspPPI	RGGWCCY	2 cut(s)	268, 1626
PsrI	GAACNNNNNNTAC	2 cut(s)	3166, 3198
PstNI	CAGNNNCTG	2 cut(s)	1504, 2383
PsuI	RGATCY	5 cut(s)	693, 1037, 1266, 1687, 1715
PsyI	GACNNNGTC	1 cut(s)	168
PvuII	CAGCTG	1 cut(s)	1751
RsaI	GTAC	7 cut(s)	44, 215, 416, 769, 1759, 2576, 3217
RsaNI	GTAC	7 cut(s)	43, 214, 415, 768, 1758, 2575, 3216
RseI	CAYNNNNRTG	4 cut(s)	852, 936, 1170, 2898
SacI	GAGCTC	1 cut(s)	1001
SapI	GCTCTTC	2 cut(s)	837, 1341
Sau96I	GGNCC	4 cut(s)	170, 228, 268, 1626
SchI	GAGTC	3 cut(s)	159, 1649, 2660
ScrFI	CCNGG	9 cut(s)	603, 657, 810, 1545, 1652, 2553, 2595, 2693, 3080
SduI	GDGCHC	5 cut(s)	849, 931, 1001, 2250, 3206
SgrAI	CRCCGGYG	1 cut(s)	247
SinI	GGWCC	4 cut(s)	170, 228, 268, 1626
SmiMI	CAYNNNNRTG	4 cut(s)	852, 936, 1170, 2898
SmlI	CTYRAG	5 cut(s)	471, 689, 1776, 2720, 3035
SmoI	CTYRAG	5 cut(s)	471, 689, 1776, 2720, 3035
SphI	GCATGC	2 cut(s)	818, 1375
SspMI	CTAG	5 cut(s)	983, 1634, 2519, 2532, 2697
SstI	GAGCTC	1 cut(s)	1001
StyD4I	CCNGG	9 cut(s)	601, 655, 808, 1543, 1650, 2551, 2593, 2691, 3078
StyI	CCWWGG	4 cut(s)	217, 516, 931, 2904
TaaI	ACNGT	8 cut(s)	538, 851, 1075, 1192, 2080, 2100, 2252, 2469
TaiI	ACGT	4 cut(s)	143, 1282, 1582, 1975
TaqI	TCGA	5 cut(s)	71, 441, 1120, 1854, 3043
TaqII	GACCGA	3 cut(s)	158, 293, 307
TatI	WGTACW	2 cut(s)	1757, 2574
TauI	GCSGC	5 cut(s)	105, 195, 240, 2429, 3140
TfiI	GAWTC	8 cut(s)	206, 325, 758, 1124, 1494, 1769, 2209, 2659
TscAI	CASTG	4 cut(s)	541, 915, 1801, 2105
TseFI	GTSAC	5 cut(s)	319, 373, 553, 893, 2458
Tsp45I	GTSAC	5 cut(s)	319, 373, 553, 893, 2458
TspRI	CASTG	4 cut(s)	541, 915, 1801, 2105
Tth111I	GACNNNGTC	1 cut(s)	168
Van91I	CCANNNNNTGG	1 cut(s)	1728
Vha464I	CTTAAG	1 cut(s)	3035
VpaK11BI	GGWCC	4 cut(s)	170, 228, 268, 1626
XapI	RAATTY	4 cut(s)	466, 739, 1329, 2744
XbaI	TCTAGA	1 cut(s)	2531
XceI	RCATGY	7 cut(s)	818, 1175, 1375, 2336, 2369, 2548, 2827
XmnI	GAANNNNTTC	1 cut(s)	798
XspI	CTAG	5 cut(s)	983, 1634, 2519, 2532, 2697
Zsp2I	ATGCAT	4 cut(s)	439, 585, 1276, 2550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.