Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003002

Physical Location & Seq

Genomic Coordinates Reverse (-)

17270391 .. 17275731

Gene Structure 5341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1082700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2028 bp

ATGGCTGCGTACGGACCTGGATTTCTTCTGGGTTTTCTTGCATTTCTGGGAATGCAGCTTCTCCCTCAAATAATGGCAGAATGCCCCTTAAATTTGAGTGGATCAAACGTTACTCAGGCAGCCTCTTTGTGCTCTAATAAAGAAGACAGGGGAAAGTGTTGCCGCTATATAAATGCTTTCATTGCAGTTTCTATTGCTCAGTATGCAAATGTGACAAGCAACTTGGGCGTTCCTTCAAATTTATCTGACATATGCCTCCATTCCATATCACAAACCTTAGTACTGTATGGAGTTCCCAGAAATGCAACAGCTTTCTGTGGATTTGGGACAAAAATTCCAGTAAATTATGAGTGTAAGGGTCGAACAACTGTCACACAAATGCTGCAGTCTCCAAAGTTCTTTGATGTCATGGCAAATTGCAAAGTACCACTTTCAGAGGAAACTAAATGCAAGAAGTGTTTAAATTCTGGCATCGTATATCTTCATCGTCTATTAGGAACAGAAGATAATATAACTTTGAGTACCTGCCGTGATGCAACTTTTGCTGCATTGGCAAGCCAACTTGATGATGCTTCAGCTATTGACATGGCAAGCTGTTTCTTTCAAATCCAGGGGCTCAACAACATCCTACCAGTTTCAGAGCCATCGCCATCCTCCACCCCGAAGGCTTCTCCAAGTCCTTTTGTGGCTGCTAGCCCAAGCCAACACTTGTTGGGTCCACCCCAAAATGAAACACATCATTCCTACCACCTTACATTGGTTCCAGGTATTGGCATTGCAGTAACAGCTGTCGCTGTAATGATGCTTTTAGTCTTGATAGTTCTCATTCGTAAGAAAAACAGAGAGTTACTGGATTCTGAGAGTATGGATCAAAAATCATCAAAATCCTTTCACTCGCCTCGTCCTATGAGGAAATTTCAGGAAGGTCCTCCATCTATGTTTCGGAAATTCAGCTACAAGGAGATAAAGAAGGCGACTGAAAACTTCAACACAATTATTGGACAAGGGGGATTTGGAACTGTGTACAAAGCTCACTTTAGTGATGGTTTAGCAATAGCAGTTAAGCGAATGAGCAAAGTGTCAGAGCAGGGAGAACATGAGTTCTGCCGAGAGATAGTGCTTCTAGCCAGATTGCATCATCGTCATCTTGTTGCTCTGAGGGGCTTTTGCAGCGAGAAGCGTGAGAGGTTTCTCATGTATGAATACATGGAAAATGGTAGCTTGAAGGACCATCTTCACTCTCCAAATAGGACTCCTCTAAGTTGGCGGACTAGAATCCATATTGCCATTGACGTAGCTAACGCTCTGGAGTATCTCCATTTCTATTGTGATCCTCCTCTTTGCCATAGAGATATTAAGTCCAGCAATATCTTACTGGATGATAATTTTGTTGCCAAGGTTGCAGATTTTGGCCTTGCACAGGCTTCAAAAGATGGTTCTATTTGCTTTGAACCAGTAAATACGGATATCCGGGGAACTCCAGGTTATATGGATCCGGAGTACGTGGTTACCCAAGAGCTGACGGAGAAAAGTGATGTATACAGCTATGGTGTGTTACTGTTGGAGCTAGTAACTGCAAGGCGAGCAATACAAGACAACAGGAATTTGGTAGAATGGTCACAAAAGTACATGGAATCTGAATTAAGCTTACCTGATCTGGTGGATCCCAGTATACGAGATTCATTTGATTTGGATCAGCTCCAAACGATTGTGTCTATCATGAGATGGTGCACCCAGAAAGAAGGCCGTGATAGGCCTTCAATCAAACAGGTGCTTAGGCTTTTGTACGAGAGTTCAGACCCAATGCATAGTGGGTTCCTAGCAGCCGTGGAAGATGAAGAGTACGAAGAGGCGGAAGGAAGAGGAAGGACTAGTAAGGGGAAGATGCATAGGACTGACATGATTTTTCATAGCGGGGATGGGAGGTATCTCGCTTCTTCTTCGAGTACATCCAGGTCTTATTGTAGTAGAAGCTTCTTGCTTGAAAATGGCTCGCCACAATCCCCTCCGAATATTCTCTCCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

676

Amino Acids

75.15

Weight (kDa)

6.85

Isoelectric Point (pI)

50.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	26 - 170	3.4e-23	SPARK
PK_Tyr_Ser-Thr	PF07714	329 - 594	1.8e-46	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	331 - 593	1e-49	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003002)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49730 AT1G49730 AT1G49730 AT1G49730 AT1G49730 AT3G19300
fragaria_vesca	FvH4_2g05840 FvH4_2g05840 FvH4_2g05840 FvH4_2g05840 FvH4_2g05840
malus_domestica	MD05G1082700.v1.1
prunus_persica	Prupe.8G127400_v2.0.a1 Prupe.8G127400_v2.0.a1 Prupe.8G127400_v2.0.a1 Prupe.8G127400_v2.0.a1 Prupe.8G127400_v2.0.a1
pyrus_communis	pycom05g07770
rosa_chinensis	RchiOBHm_Chr4g0408511 RchiOBHm_Chr6g0257781
rosa_laevigata	RLG00000014723 RLG00000014972
rosa_multiflora	Rmu_co8277925.1_g000001 Rmu_co8468953.1_g000001 Rmu_sc0003520.1_g000001
rosa_roxburghii	Rroxscaffold_5G00352930 Rroxscaffold_7G00208940
rosa_rugosa	Rorug05G0568100 Rorug05G0568200 Rorug05G0568300 Rorug05G0568400
rosa_samantha	Rh2AG563700 Rh6AG085100 Rh6AG085200 Rh6BG079500 Rh6CG074500 Rh6DG071400
rosa_wichuraiana	Rw6G007650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	533
AccB7I	CCANNNNNTGG	1 cut(s)	770
AccI	GTMKAC	2 cut(s)	1539, 1672
AccIII	TCCGGA	1 cut(s)	1495
AciI	CCGC	4 cut(s)	163, 1267, 1853, 1914
AclI	AACGTT	1 cut(s)	108
AclWI	GGATC	8 cut(s)	109, 876, 1325, 1487, 1500, 1658, 1671, 1701
AcsI	RAATTY	7 cut(s)	91, 238, 333, 463, 914, 947, 1603
AcuI	CTGAAG	1 cut(s)	558
AfiI	CCNNNNNNNGG	3 cut(s)	757, 770, 1420
AgsI	TTSAA	8 cut(s)	237, 605, 988, 1225, 1428, 1451, 1761, 1985
AhlI	ACTAGT	1 cut(s)	1871
AjnI	CCWGG	5 cut(s)	16, 609, 763, 1480, 1952
AleI	CACNNNNGTG	1 cut(s)	1038
Alw21I	GWGCWC	2 cut(s)	134, 1733
Alw26I	GTCTC	1 cut(s)	393
Alw44I	GTGCAC	1 cut(s)	1729
AlwI	GGATC	8 cut(s)	109, 876, 1325, 1487, 1500, 1658, 1671, 1701
Aor13HI	TCCGGA	1 cut(s)	1495
AoxI	GGCC	3 cut(s)	1411, 1744, 1754
ApaLI	GTGCAC	1 cut(s)	1729
ApeKI	GCWGC	8 cut(s)	5, 55, 119, 382, 545, 689, 1170, 1823
ApoI	RAATTY	7 cut(s)	91, 238, 333, 463, 914, 947, 1603
AspS9I	GGNCC	4 cut(s)	14, 716, 926, 1228
AsuC2I	CCSGG	1 cut(s)	1472
AsuNHI	GCTAGC	1 cut(s)	692
AvaII	GGWCC	4 cut(s)	14, 716, 926, 1228
BaeGI	GKGCMC	1 cut(s)	1733
BamHI	GGATCC	2 cut(s)	1492, 1663
BanII	GRGCYC	1 cut(s)	618
BbsI	GAAGAC	1 cut(s)	150
Bbv12I	GWGCWC	2 cut(s)	134, 1733
BbvI	GCAGC	7 cut(s)	67, 131, 369, 532, 676, 1182, 1835
BccI	CCATC	8 cut(s)	652, 658, 940, 1037, 1239, 1427, 1719, 1913
BceAI	ACGGC	3 cut(s)	513, 1731, 1811
BciT130I	CCWGG	5 cut(s)	18, 611, 765, 1482, 1954
BcnI	CCSGG	1 cut(s)	1472
BcoDI	GTCTC	1 cut(s)	393
BcuI	ACTAGT	1 cut(s)	1871
BfaI	CTAG	6 cut(s)	693, 1124, 1272, 1568, 1820, 1872
BfmI	CTRYAG	1 cut(s)	383
BfuAI	ACCTGC	1 cut(s)	533
BisI	GCNGC	9 cut(s)	6, 56, 120, 163, 383, 546, 690, 1171, 1824
BlsI	GCNGC	9 cut(s)	7, 57, 121, 164, 384, 547, 691, 1172, 1825
BmcAI	AGTACT	1 cut(s)	282
Bme1390I	CCNGG	6 cut(s)	18, 611, 765, 1472, 1482, 1954
Bme18I	GGWCC	4 cut(s)	14, 716, 926, 1228
BmgT120I	GGNCC	4 cut(s)	14, 716, 926, 1228
BmiI	GGNNCC	5 cut(s)	717, 762, 1494, 1665, 1817
BmrFI	CCNGG	6 cut(s)	18, 611, 765, 1472, 1482, 1954
BmrI	ACTGGG	1 cut(s)	1662
BmsI	GCATC	6 cut(s)	480, 523, 559, 792, 1144, 1875
BmtI	GCTAGC	1 cut(s)	696
BmuI	ACTGGG	1 cut(s)	1662
BpiI	GAAGAC	1 cut(s)	150
BpmI	CTGGAG	2 cut(s)	1328, 1464
Bpu10I	CCTNAGC	1 cut(s)	1775
BpuMI	CCSGG	1 cut(s)	1472
BsaAI	YACGTR	1 cut(s)	1504
BsaBI	GATNNNNATC	1 cut(s)	1692
BsaJI	CCNNGG	4 cut(s)	610, 1395, 1471, 1827
BsaWI	WCCGGW	1 cut(s)	1495
Bsc4I	CCNNNNNNNGG	3 cut(s)	757, 770, 1420
Bse1I	ACTGG	6 cut(s)	338, 632, 855, 1380, 1454, 1668
Bse3DI	GCAATG	2 cut(s)	180, 774
Bse8I	GATNNNNATC	1 cut(s)	1692
BseAI	TCCGGA	1 cut(s)	1495
BseBI	CCWGG	5 cut(s)	18, 611, 765, 1482, 1954
BseDI	CCNNGG	4 cut(s)	610, 1395, 1471, 1827
BseGI	GGATG	5 cut(s)	624, 650, 1384, 1924, 1949
BseJI	GATNNNNATC	1 cut(s)	1692
BseLI	CCNNNNNNNGG	3 cut(s)	757, 770, 1420
BseMI	GCAATG	2 cut(s)	180, 774
BseMII	CTCAG	4 cut(s)	128, 212, 849, 1148
BseNI	ACTGG	6 cut(s)	338, 632, 855, 1380, 1454, 1668
BseRI	GAGGAG	2 cut(s)	1245, 1326
BseSI	GKGCMC	1 cut(s)	1733
BseXI	GCAGC	7 cut(s)	67, 131, 369, 532, 676, 1182, 1835
BshFI	GGCC	3 cut(s)	1413, 1746, 1756
BsiHKAI	GWGCWC	2 cut(s)	134, 1733
BsiSI	CCGG	2 cut(s)	1471, 1496
BsiWI	CGTACG	1 cut(s)	9
BslFI	GGGAC	1 cut(s)	340
BslI	CCNNNNNNNGG	3 cut(s)	757, 770, 1420
BsmAI	GTCTC	1 cut(s)	393
BsmFI	GGGAC	1 cut(s)	340
BsmI	GAATGC	2 cut(s)	57, 86
BsnI	GGCC	3 cut(s)	1413, 1746, 1756
Bsp1286I	GDGCHC	3 cut(s)	134, 618, 1733
Bsp13I	TCCGGA	1 cut(s)	1495
Bsp1407I	TGTACA	1 cut(s)	1023
Bsp143I	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
BspACI	CCGC	4 cut(s)	163, 1267, 1853, 1914
BspANI	GGCC	3 cut(s)	1413, 1746, 1756
BspCNI	CTCAG	4 cut(s)	127, 211, 850, 1149
BspEI	TCCGGA	1 cut(s)	1495
BspHI	TCATGA	1 cut(s)	1719
BspLI	GGNNCC	5 cut(s)	717, 762, 1494, 1665, 1817
BspMAI	CTGCAG	1 cut(s)	387
BspMI	ACCTGC	1 cut(s)	533
BspOI	GCTAGC	1 cut(s)	696
BspPI	GGATC	8 cut(s)	109, 876, 1325, 1487, 1500, 1658, 1671, 1701
BsrDI	GCAATG	2 cut(s)	180, 774
BsrGI	TGTACA	1 cut(s)	1023
BsrI	ACTGG	6 cut(s)	338, 632, 855, 1380, 1454, 1668
BssECI	CCNNGG	4 cut(s)	610, 1395, 1471, 1827
BssMI	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
BssNAI	GTATAC	2 cut(s)	1540, 1673
BssT1I	CCWWGG	1 cut(s)	1395
Bst1107I	GTATAC	2 cut(s)	1540, 1673
Bst2UI	CCWGG	5 cut(s)	18, 611, 765, 1482, 1954
Bst4CI	ACNGT	4 cut(s)	285, 370, 1021, 1560
Bst6I	CTCTTC	3 cut(s)	1833, 1842, 1855
BstAPI	GCANNNNNTGC	1 cut(s)	542
BstAUI	TGTACA	1 cut(s)	1023
BstBAI	YACGTR	1 cut(s)	1504
BstC8I	GCNNGC	5 cut(s)	556, 592, 694, 1584, 1994
BstDEI	CTNAG	7 cut(s)	114, 198, 277, 858, 1157, 1259, 1775
BstDSI	CCRYGG	1 cut(s)	1827
BstEII	GGTNACC	1 cut(s)	1507
BstENI	CCTNNNNNAGG	1 cut(s)	1418
BstF5I	GGATG	5 cut(s)	624, 650, 1384, 1924, 1949
BstKTI	GATC	7 cut(s)	104, 871, 1333, 1495, 1657, 1666, 1696
BstMAI	GTCTC	1 cut(s)	393
BstMBI	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
BstMWI	GCNNNNNNNGC	9 cut(s)	182, 203, 225, 542, 551, 785, 1170, 1400, 1583
BstNI	CCWGG	5 cut(s)	18, 611, 765, 1482, 1954
BstPI	GGTNACC	1 cut(s)	1507
BstSCI	CCNGG	6 cut(s)	16, 609, 763, 1470, 1480, 1952
BstSFI	CTRYAG	1 cut(s)	383
BstSLI	GKGCMC	1 cut(s)	1733
BstV1I	GCAGC	7 cut(s)	67, 131, 369, 532, 676, 1182, 1835
BstV2I	GAAGAC	1 cut(s)	150
BstX2I	RGATCY	2 cut(s)	1492, 1663
BstYI	RGATCY	2 cut(s)	1492, 1663
BstZ17I	GTATAC	2 cut(s)	1540, 1673
BsuRI	GGCC	3 cut(s)	1413, 1746, 1756
BtgI	CCRYGG	1 cut(s)	1827
BtgZI	GCGATG	1 cut(s)	630
BtsCI	GGATG	5 cut(s)	624, 650, 1384, 1924, 1949
BveI	ACCTGC	1 cut(s)	533
Cac8I	GCNNGC	5 cut(s)	556, 592, 694, 1584, 1994
CciI	TCATGA	1 cut(s)	1719
Cfr13I	GGNCC	4 cut(s)	14, 716, 926, 1228
CviAII	CATG	8 cut(s)	409, 586, 1097, 1195, 1207, 1630, 1720, 1900
DdeI	CTNAG	7 cut(s)	114, 198, 277, 858, 1157, 1259, 1775
DpnI	GATC	7 cut(s)	103, 870, 1332, 1494, 1656, 1665, 1695
DpnII	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
DraI	TTTAAA	1 cut(s)	462
Eam1104I	CTCTTC	3 cut(s)	1833, 1842, 1855
EarI	CTCTTC	3 cut(s)	1833, 1842, 1855
EciI	GGCGGA	2 cut(s)	1282, 1868
Eco130I	CCWWGG	1 cut(s)	1395
Eco147I	AGGCCT	1 cut(s)	1756
Eco24I	GRGCYC	1 cut(s)	618
Eco32I	GATATC	1 cut(s)	1468
Eco47I	GGWCC	4 cut(s)	14, 716, 926, 1228
Eco57I	CTGAAG	1 cut(s)	558
Eco91I	GGTNACC	1 cut(s)	1507
EcoNI	CCTNNNNNAGG	1 cut(s)	1418
EcoO109I	RGGNCCY	1 cut(s)	926
EcoO65I	GGTNACC	1 cut(s)	1507
EcoRII	CCWGG	5 cut(s)	16, 609, 763, 1480, 1952
EcoRV	GATATC	1 cut(s)	1468
EcoT14I	CCWWGG	1 cut(s)	1395
EcoT22I	ATGCAT	2 cut(s)	1809, 1890
EcoT38I	GRGCYC	1 cut(s)	618
ErhI	CCWWGG	1 cut(s)	1395
FaeI	CATG	8 cut(s)	412, 589, 1100, 1198, 1210, 1633, 1723, 1903
FalI	AAGNNNNNCTT	2 cut(s)	414, 446
FaqI	GGGAC	1 cut(s)	340
FatI	CATG	8 cut(s)	408, 585, 1096, 1194, 1206, 1629, 1719, 1899
FauI	CCCGC	1 cut(s)	1907
FauNDI	CATATG	1 cut(s)	251
FblI	GTMKAC	2 cut(s)	1539, 1672
Fnu4HI	GCNGC	9 cut(s)	6, 56, 120, 163, 383, 546, 690, 1171, 1824
FokI	GGATG	5 cut(s)	611, 637, 1391, 1931, 1936
FriOI	GRGCYC	1 cut(s)	618
Fsp4HI	GCNGC	9 cut(s)	6, 56, 120, 163, 383, 546, 690, 1171, 1824
FspBI	CTAG	6 cut(s)	693, 1124, 1272, 1568, 1820, 1872
GluI	GCNGC	9 cut(s)	6, 56, 120, 163, 383, 546, 690, 1171, 1824
GsuI	CTGGAG	2 cut(s)	1328, 1464
HaeIII	GGCC	3 cut(s)	1413, 1746, 1756
HapII	CCGG	2 cut(s)	1471, 1496
Hin1II	CATG	8 cut(s)	412, 589, 1100, 1198, 1210, 1633, 1723, 1903
HindIII	AAGCTT	2 cut(s)	1645, 1972
HinfI	GANTC	5 cut(s)	854, 1252, 1275, 1634, 1679
HpaII	CCGG	2 cut(s)	1471, 1496
Hpy166II	GTNNAC	5 cut(s)	719, 1024, 1540, 1673, 1731
Hpy188III	TCNNGA	5 cut(s)	814, 920, 1307, 1496, 1720
Hpy8I	GTNNAC	5 cut(s)	719, 1024, 1540, 1673, 1731
HpyAV	CCTTC	9 cut(s)	243, 658, 917, 964, 1219, 1736, 1767, 1850, 1860
HpyCH4III	ACNGT	4 cut(s)	285, 370, 1021, 1560
HpyCH4IV	ACGT	3 cut(s)	108, 1293, 1503
HpyF10VI	GCNNNNNNNGC	9 cut(s)	182, 203, 225, 542, 551, 785, 1170, 1400, 1583
HpyF3I	CTNAG	7 cut(s)	114, 198, 277, 858, 1157, 1259, 1775
HpySE526I	ACGT	3 cut(s)	108, 1293, 1503
Hsp92II	CATG	8 cut(s)	412, 589, 1100, 1198, 1210, 1633, 1723, 1903
Kpn2I	TCCGGA	1 cut(s)	1495
Kzo9I	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
LmnI	GCTCC	2 cut(s)	1564, 1704
Lsp1109I	GCAGC	7 cut(s)	67, 131, 369, 532, 676, 1182, 1835
LweI	GCATC	6 cut(s)	480, 523, 559, 792, 1144, 1875
MaeI	CTAG	6 cut(s)	693, 1124, 1272, 1568, 1820, 1872
MaeII	ACGT	3 cut(s)	108, 1293, 1503
MaeIII	GTNAC	9 cut(s)	109, 211, 370, 781, 846, 1507, 1554, 1570, 1617
MalI	GATC	7 cut(s)	103, 870, 1332, 1494, 1656, 1665, 1695
MboI	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
MflI	RGATCY	2 cut(s)	1492, 1663
MhlI	GDGCHC	3 cut(s)	134, 618, 1733
MlyI	GAGTC	1 cut(s)	1246
MmeI	TCCRAC	1 cut(s)	1542
Mph1103I	ATGCAT	2 cut(s)	1809, 1890
MroI	TCCGGA	1 cut(s)	1495
MseI	TTAA	5 cut(s)	89, 461, 1062, 1356, 1643
MslI	CAYNNNNRTG	2 cut(s)	377, 1038
MspA1I	CMGCKG	1 cut(s)	788
MspI	CCGG	2 cut(s)	1471, 1496
MspR9I	CCNGG	6 cut(s)	18, 611, 765, 1472, 1482, 1954
Mva1269I	GAATGC	2 cut(s)	57, 86
MvaI	CCWGG	5 cut(s)	18, 611, 765, 1482, 1954
MwoI	GCNNNNNNNGC	9 cut(s)	182, 203, 225, 542, 551, 785, 1170, 1400, 1583
NciI	CCSGG	1 cut(s)	1472
NdeI	CATATG	1 cut(s)	251
NdeII	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
NheI	GCTAGC	1 cut(s)	692
NlaIII	CATG	8 cut(s)	412, 589, 1100, 1198, 1210, 1633, 1723, 1903
NlaIV	GGNNCC	5 cut(s)	717, 762, 1494, 1665, 1817
NmeAIII	GCCGAG	1 cut(s)	1133
NmuCI	GTSAC	3 cut(s)	211, 370, 1617
NsiI	ATGCAT	2 cut(s)	1809, 1890
OliI	CACNNNNGTG	1 cut(s)	1038
PagI	TCATGA	1 cut(s)	1719
PceI	AGGCCT	1 cut(s)	1756
PctI	GAATGC	2 cut(s)	57, 86
PfeI	GAWTC	4 cut(s)	854, 1275, 1634, 1679
Pfl23II	CGTACG	1 cut(s)	9
PflMI	CCANNNNNTGG	1 cut(s)	770
PkrI	GCNGC	9 cut(s)	7, 57, 121, 164, 384, 547, 691, 1172, 1825
PleI	GAGTC	1 cut(s)	1246
PpsI	GAGTC	1 cut(s)	1246
Ppu21I	YACGTR	1 cut(s)	1504
PpuMI	RGGWCCY	1 cut(s)	926
Psp1406I	AACGTT	1 cut(s)	108
Psp5II	RGGWCCY	1 cut(s)	926
Psp6I	CCWGG	5 cut(s)	16, 609, 763, 1480, 1952
PspEI	GGTNACC	1 cut(s)	1507
PspGI	CCWGG	5 cut(s)	16, 609, 763, 1480, 1952
PspLI	CGTACG	1 cut(s)	9
PspN4I	GGNNCC	5 cut(s)	717, 762, 1494, 1665, 1817
PspPI	GGNCC	4 cut(s)	14, 716, 926, 1228
PspPPI	RGGWCCY	1 cut(s)	926
PstI	CTGCAG	1 cut(s)	387
PsuI	RGATCY	2 cut(s)	1492, 1663
PvuII	CAGCTG	1 cut(s)	788
RseI	CAYNNNNRTG	2 cut(s)	377, 1038
SaqAI	TTAA	5 cut(s)	89, 461, 1062, 1356, 1643
SatI	GCNGC	9 cut(s)	6, 56, 120, 163, 383, 546, 690, 1171, 1824
Sau3AI	GATC	7 cut(s)	101, 868, 1330, 1492, 1654, 1663, 1693
Sau96I	GGNCC	4 cut(s)	14, 716, 926, 1228
ScaI	AGTACT	1 cut(s)	282
SchI	GAGTC	1 cut(s)	1246
ScrFI	CCNGG	6 cut(s)	18, 611, 765, 1472, 1482, 1954
SduI	GDGCHC	3 cut(s)	134, 618, 1733
SfaNI	GCATC	6 cut(s)	480, 523, 559, 792, 1144, 1875
SfcI	CTRYAG	1 cut(s)	383
SinI	GGWCC	4 cut(s)	14, 716, 926, 1228
SmiMI	CAYNNNNRTG	2 cut(s)	377, 1038
SpeI	ACTAGT	1 cut(s)	1871
SseBI	AGGCCT	1 cut(s)	1756
SsiI	CCGC	4 cut(s)	163, 1267, 1853, 1914
SspI	AATATT	1 cut(s)	2014
SspMI	CTAG	6 cut(s)	693, 1124, 1272, 1568, 1820, 1872
StuI	AGGCCT	1 cut(s)	1756
StyD4I	CCNGG	6 cut(s)	16, 609, 763, 1470, 1480, 1952
StyI	CCWWGG	1 cut(s)	1395
TaaI	ACNGT	4 cut(s)	285, 370, 1021, 1560
TaiI	ACGT	3 cut(s)	111, 1296, 1506
TaqI	TCGA	2 cut(s)	361, 1943
TatI	WGTACW	4 cut(s)	280, 1023, 1626, 1946
TauI	GCSGC	1 cut(s)	165
TfiI	GAWTC	4 cut(s)	854, 1275, 1634, 1679
Tru1I	TTAA	5 cut(s)	89, 461, 1062, 1356, 1643
Tru9I	TTAA	5 cut(s)	89, 461, 1062, 1356, 1643
TseFI	GTSAC	3 cut(s)	211, 370, 1617
TseI	GCWGC	8 cut(s)	5, 55, 119, 382, 545, 689, 1170, 1823
Tsp45I	GTSAC	3 cut(s)	211, 370, 1617
TspDTI	ATGAA	7 cut(s)	169, 473, 744, 1215, 1671, 1851, 1898
TspGWI	ACGGA	4 cut(s)	27, 1478, 1538, 2011
Van91I	CCANNNNNTGG	1 cut(s)	770
VneI	GTGCAC	1 cut(s)	1729
VpaK11BI	GGWCC	4 cut(s)	14, 716, 926, 1228
XagI	CCTNNNNNAGG	1 cut(s)	1418
XapI	RAATTY	7 cut(s)	91, 238, 333, 463, 914, 947, 1603
XmiI	GTMKAC	2 cut(s)	1539, 1672
XspI	CTAG	6 cut(s)	693, 1124, 1272, 1568, 1820, 1872
ZrmI	AGTACT	1 cut(s)	282
Zsp2I	ATGCAT	2 cut(s)	1809, 1890

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.