MD05G1133800.v1.1
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003894
Physical Location & Seq
Forward (+)
26243662 .. 26245161
1500 bp
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UTR
Exon/CDS
Intron
MD05G1133800.v1.1.491

Sequence Viewer

Length: 417 bp
ATGCGGGATGGTACACCACGTGTCATTATACAATTGCTGCTTGAGGAAAACAGCATACCACATGATAATGTTCGGATTTGCTACTCCTCGTTTTCGAGTACAACTCACACTGCTCAAGTTGTTGCATCTGTCTTGAACATTCCATTTGAGGGTGCACAATGCAAGTATCACGACATATGGGCTCTTGATGAGAAATATCCACTCATGAGGCCGGCAGAGGGAGGAGAAAGCGTCGATGATGTTGCATGCGGACTTGCAGAAGCAATGGAAACTATGGAGTCACAATTTCAAGGGTGCACGATCTTAGTTGTTAGTCACGGCGATACCCTACAAATTTTACTGACGATACTCAATGCTGCCAAGCTAAACGCAGGATCCAGTCACACTGATCTGTCATCAAGAATTCAAGCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

139

Amino Acids

15.02

Weight (kDa)

4.76

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 56 - 124 3.4e-07 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 4, 249
AclWI GGATC 2 cut(s) 369, 382
AcsI RAATTY 2 cut(s) 333, 402
AcvI CACGTG 1 cut(s) 20
AdeI CACNNNGTG 1 cut(s) 20
AfaI GTAC 2 cut(s) 13, 100
AfiI CCNNNNNNNGG 2 cut(s) 149, 218
AflIII ACRYGT 1 cut(s) 19
AgsI TTSAA 3 cut(s) 136, 290, 407
AluBI AGCT 1 cut(s) 364
AluI AGCT 1 cut(s) 364
Alw21I GWGCWC 2 cut(s) 157, 299
Alw44I GTGCAC 2 cut(s) 153, 295
AlwI GGATC 2 cut(s) 369, 382
AoxI GGCC 1 cut(s) 209
ApaLI GTGCAC 2 cut(s) 153, 295
ApeKI GCWGC 2 cut(s) 37, 356
ApoI RAATTY 2 cut(s) 333, 402
BaeGI GKGCMC 2 cut(s) 157, 299
BamHI GGATCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 184
BbrPI CACGTG 1 cut(s) 20
Bbv12I GWGCWC 2 cut(s) 157, 299
BbvI GCAGC 2 cut(s) 24, 343
BccI CCATC 1 cut(s) 2
BceAI ACGGC 1 cut(s) 334
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BisI GCNGC 2 cut(s) 38, 357
BlsI GCNGC 2 cut(s) 39, 358
BmiI GGNNCC 1 cut(s) 376
BmsI GCATC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpuEI CTTGAG 2 cut(s) 62, 99
BsaAI YACGTR 1 cut(s) 20
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 218
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 270
BseGI GGATG 1 cut(s) 13
BseLI CCNNNNNNNGG 2 cut(s) 149, 218
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 2 cut(s) 76, 237
BseSI GKGCMC 2 cut(s) 157, 299
BseXI GCAGC 2 cut(s) 24, 343
BshFI GGCC 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 157, 299
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 149, 218
BsnI GGCC 1 cut(s) 211
Bsp1286I GDGCHC 3 cut(s) 157, 184, 299
Bsp143I GATC 3 cut(s) 300, 374, 388
BspACI CCGC 2 cut(s) 4, 249
BspANI GGCC 1 cut(s) 211
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 376
BspPI GGATC 2 cut(s) 369, 382
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 211
BssMI GATC 3 cut(s) 300, 374, 388
BstBAI YACGTR 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 213, 247
BstDEI CTNAG 1 cut(s) 304
BstF5I GGATG 1 cut(s) 13
BstKTI GATC 3 cut(s) 303, 377, 391
BstMBI GATC 3 cut(s) 300, 374, 388
BstNSI RCATGY 1 cut(s) 249
BstSLI GKGCMC 2 cut(s) 157, 299
BstV1I GCAGC 2 cut(s) 24, 343
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BsuRI GGCC 1 cut(s) 211
BtsCI GGATG 1 cut(s) 13
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 108, 384
Cac8I GCNNGC 2 cut(s) 213, 247
CciI TCATGA 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 211
CseI GACGC 1 cut(s) 220
Csp6I GTAC 2 cut(s) 12, 99
CviAII CATG 3 cut(s) 62, 205, 246
CviJI RGCY 3 cut(s) 182, 211, 364
CviKI_1 RGCY 3 cut(s) 182, 211, 364
CviQI GTAC 2 cut(s) 12, 99
DdeI CTNAG 1 cut(s) 304
DpnI GATC 3 cut(s) 302, 376, 390
DpnII GATC 3 cut(s) 300, 374, 388
DraIII CACNNNGTG 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 184
Eco72I CACGTG 1 cut(s) 20
EcoRI GAATTC 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 184
FaeI CATG 3 cut(s) 65, 208, 249
FaiI YATR 8 cut(s) 29, 56, 63, 176, 178, 206, 247, 275
FatI CATG 3 cut(s) 61, 204, 245
FauNDI CATATG 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 38, 357
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 2 cut(s) 38, 357
GluI GCNGC 2 cut(s) 38, 357
HaeIII GGCC 1 cut(s) 211
HapII CCGG 1 cut(s) 212
HgaI GACGC 1 cut(s) 220
Hin1II CATG 3 cut(s) 65, 208, 249
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 212
Hpy166II GTNNAC 3 cut(s) 14, 155, 297
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 5 cut(s) 133, 170, 185, 205, 399
Hpy8I GTNNAC 3 cut(s) 14, 155, 297
Hpy99I CGWCG 1 cut(s) 236
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 6 cut(s) 125, 155, 162, 245, 257, 297
HpyF3I CTNAG 1 cut(s) 304
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 3 cut(s) 65, 208, 249
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 300, 374, 388
LpnPI CCDG 3 cut(s) 225, 357, 391
Lsp1109I GCAGC 2 cut(s) 24, 343
LweI GCATC 1 cut(s) 134
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 3 cut(s) 279, 314, 380
MalI GATC 3 cut(s) 302, 376, 390
MboI GATC 3 cut(s) 300, 374, 388
MfeI CAATTG 1 cut(s) 32
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 3 cut(s) 157, 184, 299
MluCI AATT 4 cut(s) 32, 284, 333, 402
MlyI GAGTC 1 cut(s) 287
MnlI CCTC 6 cut(s) 37, 97, 142, 201, 211, 215
MroNI GCCGGC 1 cut(s) 211
MslI CAYNNNNRTG 1 cut(s) 66
MspI CCGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 32
NaeI GCCGGC 1 cut(s) 213
NdeI CATATG 1 cut(s) 176
NdeII GATC 3 cut(s) 300, 374, 388
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 3 cut(s) 65, 208, 249
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 3 cut(s) 279, 314, 380
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 204
PdiI GCCGGC 1 cut(s) 213
PkrI GCNGC 2 cut(s) 39, 358
PleI GAGTC 1 cut(s) 286
PmaCI CACGTG 1 cut(s) 20
PmlI CACGTG 1 cut(s) 20
PpsI GAGTC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 20
PspCI CACGTG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 376
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 2 cut(s) 13, 100
RsaNI GTAC 2 cut(s) 12, 99
RseI CAYNNNNRTG 1 cut(s) 66
SatI GCNGC 2 cut(s) 38, 357
Sau3AI GATC 3 cut(s) 300, 374, 388
SchI GAGTC 1 cut(s) 287
SduI GDGCHC 3 cut(s) 157, 184, 299
SetI ASST 2 cut(s) 22, 366
SfaNI GCATC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 2 cut(s) 41, 114
SmoI CTYRAG 2 cut(s) 41, 114
SphI GCATGC 1 cut(s) 249
Sse9I AATT 4 cut(s) 32, 284, 333, 402
SsiI CCGC 2 cut(s) 4, 249
TaiI ACGT 1 cut(s) 22
TaqI TCGA 2 cut(s) 95, 234
TasI AATT 4 cut(s) 32, 284, 333, 402
TatI WGTACW 1 cut(s) 98
TscAI CASTG 2 cut(s) 115, 391
TseFI GTSAC 3 cut(s) 279, 314, 380
TseI GCWGC 2 cut(s) 37, 356
Tsp45I GTSAC 3 cut(s) 279, 314, 380
TspRI CASTG 2 cut(s) 115, 391
VneI GTGCAC 2 cut(s) 153, 295
XapI RAATTY 2 cut(s) 333, 402
XceI RCATGY 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.