Rh6DG239800
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003894
Physical Location & Seq
Forward (+)
42703537 .. 42706074
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG239800.1

Sequence Viewer

Length: 498 bp
ATGGCGGAGACGACGTCGTTTGCGAGGAACAGATACTGGGTACTGAGGCACGGCAAGAGCATTCCGAATGAGAAAGGCCTAATCGTCTCCTCCATGGAAAATGGGACGCGCCCAGAGTATCAATTGGCGCCGGAAGGTGTTGATCAAGCAAAACGGGCTGGAGAGTTGTTCCGAAAGGTACTTGAGGAAAATAGCATGCCAATTGAAAATGTTCGCATTTGCTATTCTCCCTTTTCGAGAACAAGACAGACTGCTGAAGTTGTTGCATCTGTCTTGAATATTCAGTTTGAGGGTGCTCAATGTAAGGTGATGGAAAACCTCCGCGAACGCTTCTTTGGTCCTTCATATGAGCTCATGTCACATGATAAATATCACGAAGTATGGGCTATTGATGAGAAAGATCCACTCATGAAGCCAGAAGGAGGAGAAAGTGTCGATGATGTTGCATCTAGACTTGTAGAGGCTATTGCAAACATAGAATCACTATTTCAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

165

Amino Acids

18.77

Weight (kDa)

5.2

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 13 - 162 7.8e-17 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 127
AccII CGCG 2 cut(s) 109, 324
AciI CCGC 2 cut(s) 5, 322
AclWI GGATC 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 2 cut(s) 14, 128
AfaI GTAC 2 cut(s) 42, 180
AfiI CCNNNNNNNGG 1 cut(s) 422
AgsI TTSAA 3 cut(s) 206, 277, 491
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 1 cut(s) 352
AluI AGCT 1 cut(s) 352
Alw21I GWGCWC 2 cut(s) 298, 354
Alw26I GTCTC 2 cut(s) 2, 91
AlwI GGATC 1 cut(s) 395
AlwNI CAGNNNCTG 1 cut(s) 36
AoxI GGCC 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 210
AspLEI GCGC 2 cut(s) 111, 130
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 354
Bbv12I GWGCWC 2 cut(s) 298, 354
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 2, 91
BfaI CTAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 129
BmrI ACTGGG 1 cut(s) 46
BmsI GCATC 2 cut(s) 275, 455
BmuI ACTGGG 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 14, 128
BsaJI CCNNGG 1 cut(s) 93
BsaXI ACNNNNNCTCC 3 cut(s) 29, 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse1I ACTGG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 93
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 422
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 1 cut(s) 41
BseRI GAGGAG 2 cut(s) 79, 438
Bsh1236I CGCG 2 cut(s) 109, 324
BshFI GGCC 1 cut(s) 78
BshNI GGYRCC 1 cut(s) 127
BsiHKAI GWGCWC 2 cut(s) 298, 354
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 422
BsmAI GTCTC 2 cut(s) 2, 91
BsmBI CGTCTC 2 cut(s) 2, 91
BsmFI GGGAC 1 cut(s) 118
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 298, 354
Bsp143I GATC 2 cut(s) 142, 400
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 2 cut(s) 5, 322
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 2 cut(s) 109, 324
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 127
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 2 cut(s) 142, 400
BssNI GRCGYC 2 cut(s) 14, 128
BssT1I CCWWGG 1 cut(s) 93
BstACI GRCGYC 2 cut(s) 14, 128
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 109, 324
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 111, 130
BstKTI GATC 2 cut(s) 145, 403
BstMAI GTCTC 2 cut(s) 2, 91
BstMBI GATC 2 cut(s) 142, 400
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNSI RCATGY 1 cut(s) 199
BstUI CGCG 2 cut(s) 109, 324
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 197
CaiI CAGNNNCTG 1 cut(s) 36
CciI TCATGA 1 cut(s) 408
CfoI GCGC 2 cut(s) 111, 130
Cfr13I GGNCC 1 cut(s) 338
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 41, 179
CviAII CATG 5 cut(s) 94, 196, 355, 362, 409
CviJI RGCY 6 cut(s) 78, 158, 352, 386, 415, 464
CviKI_1 RGCY 6 cut(s) 78, 158, 352, 386, 415, 464
CviQI GTAC 2 cut(s) 41, 179
DdeI CTNAG 1 cut(s) 44
DinI GGCGCC 1 cut(s) 129
DpnI GATC 2 cut(s) 144, 402
DpnII GATC 2 cut(s) 142, 400
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 93
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 354
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 93
Esp3I CGTCTC 2 cut(s) 2, 91
FaeI CATG 5 cut(s) 97, 199, 358, 365, 412
FaiI YATR 9 cut(s) 95, 197, 346, 348, 356, 363, 382, 410, 476
FaqI GGGAC 1 cut(s) 118
FatI CATG 5 cut(s) 93, 195, 354, 361, 408
FauNDI CATATG 1 cut(s) 346
FbaI TGATCA 1 cut(s) 142
FriOI GRGCYC 1 cut(s) 354
FspBI CTAG 1 cut(s) 450
GlaI GCGC 2 cut(s) 110, 129
GsuI CTGGAG 1 cut(s) 180
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 131
HgaI GACGC 1 cut(s) 115
HhaI GCGC 2 cut(s) 111, 130
Hin1I GRCGYC 2 cut(s) 14, 128
Hin1II CATG 5 cut(s) 97, 199, 358, 365, 412
Hin6I GCGC 2 cut(s) 109, 128
HinP1I GCGC 2 cut(s) 109, 128
HinfI GANTC 1 cut(s) 479
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 2 cut(s) 66, 173
Hpy188III TCNNGA 5 cut(s) 237, 274, 374, 409, 450
Hpy99I CGWCG 2 cut(s) 16, 19
HpyAV CCTTC 3 cut(s) 128, 351, 413
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 3 cut(s) 266, 446, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 1 cut(s) 14
Hsp92I GRCGYC 2 cut(s) 14, 128
Hsp92II CATG 5 cut(s) 97, 199, 358, 365, 412
HspAI GCGC 2 cut(s) 109, 128
KasI GGCGCC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 2 cut(s) 142, 400
LpnPI CCDG 5 cut(s) 22, 126, 144, 144, 429
LweI GCATC 2 cut(s) 275, 455
MaeI CTAG 1 cut(s) 450
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 357
MalI GATC 2 cut(s) 144, 402
MboI GATC 2 cut(s) 142, 400
MfeI CAATTG 2 cut(s) 122, 201
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 298, 354
MluCI AATT 2 cut(s) 122, 201
Mly113I GGCGCC 1 cut(s) 128
MnlI CCTC 8 cut(s) 18, 39, 100, 178, 283, 329, 416, 454
MroXI GAANNNNTTC 1 cut(s) 210
MspI CCGG 1 cut(s) 131
MunI CAATTG 2 cut(s) 122, 201
Mva1269I GAATGC 1 cut(s) 60
MvnI CGCG 2 cut(s) 109, 324
MwoI GCNNNNNNNGC 1 cut(s) 155
NarI GGCGCC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 346
NdeII GATC 2 cut(s) 142, 400
NlaIII CATG 5 cut(s) 97, 199, 358, 365, 412
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 357
NspI RCATGY 1 cut(s) 199
PaeI GCATGC 1 cut(s) 199
PagI TCATGA 1 cut(s) 408
PceI AGGCCT 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 479
PflFI GACNNNGTC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 338
PstNI CAGNNNCTG 1 cut(s) 36
PsuI RGATCY 1 cut(s) 400
PsyI GACNNNGTC 1 cut(s) 13
RsaI GTAC 2 cut(s) 42, 180
RsaNI GTAC 2 cut(s) 41, 179
SacI GAGCTC 1 cut(s) 354
Sau3AI GATC 2 cut(s) 142, 400
Sau96I GGNCC 1 cut(s) 338
SduI GDGCHC 2 cut(s) 298, 354
SetI ASST 6 cut(s) 17, 139, 180, 309, 321, 354
SfaNI GCATC 2 cut(s) 275, 455
SfoI GGCGCC 1 cut(s) 129
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SphI GCATGC 1 cut(s) 199
Sse9I AATT 2 cut(s) 122, 201
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 2 cut(s) 5, 322
SspDI GGCGCC 1 cut(s) 127
SspI AATATT 1 cut(s) 280
SspMI CTAG 1 cut(s) 450
SstI GAGCTC 1 cut(s) 354
StuI AGGCCT 1 cut(s) 78
StyI CCWWGG 1 cut(s) 93
TaiI ACGT 1 cut(s) 17
TaqI TCGA 2 cut(s) 236, 435
TasI AATT 2 cut(s) 122, 201
TfiI GAWTC 1 cut(s) 479
TseFI GTSAC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 2 cut(s) 333, 425
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 449
XceI RCATGY 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 1 cut(s) 450
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.