pycom05g13060
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003894
Physical Location & Seq
Forward (+)
16624894 .. 16625844
951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13060.1

Sequence Viewer

Length: 336 bp
ATGTCACACGATAAATATCACGACATATGGGCTCTTGATGAGAAAGATCCACTCATGAGACCGGCAGAGGGAGGAGAAAGCATCGATGATGTTGCGTGCAGACTTGCAGAAGCAATGGAAACTATGGAGTCACAATTTCAAGGGTGCACGATCTTAGTTGTTAGTCACGACGATACCCTACAAATCTTGCGGACAATACTCAATGCTGCCAAGCTAAACGCAGGATCCAGTCACACTGATCTGTCATCAAGAATTCAAGCGGTTCGAAACCCTCTCATCTTATCACAGCACTGGAAATTTTCCCTGCTTACTGCAGAGCTCCGGACTGTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

112

Amino Acids

12.43

Weight (kDa)

5.19

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 4 - 73 3.4e-08 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 321
AciI CCGC 2 cut(s) 190, 260
AclWI GGATC 3 cut(s) 41, 219, 232
AcsI RAATTY 2 cut(s) 252, 296
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 2 cut(s) 140, 257
AluBI AGCT 2 cut(s) 214, 319
AluI AGCT 2 cut(s) 214, 319
Alw21I GWGCWC 2 cut(s) 149, 321
Alw26I GTCTC 1 cut(s) 52
Alw44I GTGCAC 1 cut(s) 145
AlwI GGATC 3 cut(s) 41, 219, 232
Aor13HI TCCGGA 1 cut(s) 321
ApaLI GTGCAC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 2 cut(s) 252, 296
AsuII TTCGAA 1 cut(s) 265
BaeGI GKGCMC 1 cut(s) 149
BamHI GGATCC 1 cut(s) 224
BanII GRGCYC 2 cut(s) 34, 321
Bbv12I GWGCWC 2 cut(s) 149, 321
BbvI GCAGC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BcoDI GTCTC 1 cut(s) 52
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
BmiI GGNNCC 1 cut(s) 226
BmsI GCATC 1 cut(s) 90
Bpu14I TTCGAA 1 cut(s) 265
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 228, 296
Bse3DI GCAATG 1 cut(s) 120
Bse8I GATNNNNATC 1 cut(s) 15
BseAI TCCGGA 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 84
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 120
BseNI ACTGG 2 cut(s) 228, 296
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 118
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 149, 321
BsiSI CCGG 2 cut(s) 62, 322
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 52
Bso31I GGTCTC 1 cut(s) 52
Bsp119I TTCGAA 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 34, 149, 321
Bsp13I TCCGGA 1 cut(s) 321
Bsp143I GATC 4 cut(s) 46, 150, 224, 238
BspACI CCGC 2 cut(s) 190, 260
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 321
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 226
BspMAI CTGCAG 1 cut(s) 316
BspPI GGATC 3 cut(s) 41, 219, 232
BspT104I TTCGAA 1 cut(s) 265
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 228, 296
BssAI RCCGGY 1 cut(s) 61
BssMI GATC 4 cut(s) 46, 150, 224, 238
Bst4CI ACNGT 1 cut(s) 328
BstBI TTCGAA 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 1 cut(s) 154
BstKTI GATC 4 cut(s) 49, 153, 227, 241
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 4 cut(s) 46, 150, 224, 238
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 46, 224
BstYI RGATCY 2 cut(s) 46, 224
Bsu15I ATCGAT 1 cut(s) 84
BsuTUI ATCGAT 1 cut(s) 84
BtsIMutI CAGTG 2 cut(s) 234, 289
Cac8I GCNNGC 1 cut(s) 97
CciI TCATGA 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 61
ClaI ATCGAT 1 cut(s) 84
CviAII CATG 1 cut(s) 55
CviJI RGCY 3 cut(s) 32, 214, 319
CviKI_1 RGCY 3 cut(s) 32, 214, 319
DdeI CTNAG 1 cut(s) 154
DpnI GATC 4 cut(s) 48, 152, 226, 240
DpnII GATC 4 cut(s) 46, 150, 224, 238
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 2 cut(s) 34, 321
Eco31I GGTCTC 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 252
EcoT38I GRGCYC 2 cut(s) 34, 321
FaeI CATG 1 cut(s) 58
FaiI YATR 5 cut(s) 26, 28, 56, 125, 334
FatI CATG 1 cut(s) 54
FauNDI CATATG 1 cut(s) 26
Fnu4HI GCNGC 1 cut(s) 207
FriOI GRGCYC 2 cut(s) 34, 321
Fsp4HI GCNGC 1 cut(s) 207
GluI GCNGC 1 cut(s) 207
HapII CCGG 2 cut(s) 62, 322
Hin1II CATG 1 cut(s) 58
HinfI GANTC 1 cut(s) 128
HpaII CCGG 2 cut(s) 62, 322
Hpy166II GTNNAC 1 cut(s) 147
Hpy188III TCNNGA 6 cut(s) 20, 35, 55, 167, 249, 322
Hpy8I GTNNAC 1 cut(s) 147
Hpy99I CGWCG 1 cut(s) 173
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4V TGCA 4 cut(s) 99, 107, 147, 314
HpyF3I CTNAG 1 cut(s) 154
Hsp92II CATG 1 cut(s) 58
Kpn2I TCCGGA 1 cut(s) 321
Kzo9I GATC 4 cut(s) 46, 150, 224, 238
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 5 cut(s) 75, 207, 241, 277, 317
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 1 cut(s) 90
MaeIII GTNAC 4 cut(s) 3, 129, 164, 230
MalI GATC 4 cut(s) 48, 152, 226, 240
MboI GATC 4 cut(s) 46, 150, 224, 238
MflI RGATCY 2 cut(s) 46, 224
MhlI GDGCHC 3 cut(s) 34, 149, 321
MluCI AATT 3 cut(s) 134, 252, 296
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 3 cut(s) 61, 65, 282
MroI TCCGGA 1 cut(s) 321
MspI CCGG 2 cut(s) 62, 322
NdeI CATATG 1 cut(s) 26
NdeII GATC 4 cut(s) 46, 150, 224, 238
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 226
NmuCI GTSAC 4 cut(s) 3, 129, 164, 230
NspV TTCGAA 1 cut(s) 265
PagI TCATGA 1 cut(s) 54
PkrI GCNGC 1 cut(s) 208
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 226
PstI CTGCAG 1 cut(s) 316
PsuI RGATCY 2 cut(s) 46, 224
SacI GAGCTC 1 cut(s) 321
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 4 cut(s) 46, 150, 224, 238
SchI GAGTC 1 cut(s) 137
SduI GDGCHC 3 cut(s) 34, 149, 321
SetI ASST 2 cut(s) 216, 321
SfaNI GCATC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 265
Sse9I AATT 3 cut(s) 134, 252, 296
SsiI CCGC 2 cut(s) 190, 260
SstI GAGCTC 1 cut(s) 321
TaaI ACNGT 1 cut(s) 328
TaqI TCGA 2 cut(s) 84, 265
TasI AATT 3 cut(s) 134, 252, 296
TscAI CASTG 2 cut(s) 241, 296
TseFI GTSAC 4 cut(s) 3, 129, 164, 230
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 4 cut(s) 3, 129, 164, 230
TspRI CASTG 2 cut(s) 241, 296
VneI GTGCAC 1 cut(s) 145
XapI RAATTY 2 cut(s) 252, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.