MD10G1138800.v1.1
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003894
Physical Location & Seq
Forward (+)
22373834 .. 22375768
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1138800.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGCGGAGACGACGTCGTTTCTGAGGAACAGGTACTGGGTGCTGAGGCACGGGAAGAGCATTCCGAATGAGAGAGGCATCATTGTTTCTTCCATGGAAAATGGTACCCGCCCAGAATATCAATTGGCGCCAGAAGGCGTAAGTCAGGCACAATCAGCTGGACAATCATTCCAAAAGGTGCTTGAGGAAAACAACGTCCCACTTGATAATGTTCGGATTTGCTACTCTCCGTTTTCGAGAACAAGTCAGACTGCTCAAGTTGTTGCATCTGTCTTGAACATTCCATTTGAGGGGGCTCAATGCAAGGTGATGGAAAATCTTCGCGAACGCTTCTTTGGTCCTTCGTATGAGCTTTTGTCACATGATAAATATCACGATATATGGGCTCTTGACCAGAAAGATCCACTCATGAGGCCGGCAGAGGGAGGAGAGAGTGTCGATGATGTTGCGTGCAGACTTGCAGAAGCAATGGAAACTATGGAGTCACAATTTCAAGGGTGCATGATCTTAGTTGTTAGTCACGGCGATACCCTACAAATCTTGCAGACGATACTCAATGCTGCCAAGCTAAACGCGGGATCCAGTTACACTGACCTGTCATCAAGAATTCAAGCAGTTCGAACCCCTCCCATCTTATCACAGCACCGGAAGTTTTCCCTGCTTACTGCAGAGCTCCGGGCAGTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

230

Amino Acids

25.59

Weight (kDa)

5.77

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 13 - 191 6.9e-22 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
Acc65I GGTACC 1 cut(s) 104
AccB1I GGYRCC 2 cut(s) 104, 127
AccII CGCG 2 cut(s) 324, 575
AciI CCGC 3 cut(s) 5, 109, 575
AclWI GGATC 3 cut(s) 395, 573, 586
AcsI RAATTY 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 14, 128
AfaI GTAC 2 cut(s) 35, 106
AfiI CCNNNNNNNGG 2 cut(s) 290, 422
AgsI TTSAA 3 cut(s) 277, 494, 611
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 4 cut(s) 158, 352, 568, 673
AluI AGCT 4 cut(s) 158, 352, 568, 673
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 3 cut(s) 395, 573, 586
AlwNI CAGNNNCTG 1 cut(s) 36
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 606
Asp700I GAANNNNTTC 1 cut(s) 318
Asp718I GGTACC 1 cut(s) 104
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 619
AvaII GGWCC 1 cut(s) 338
BamHI GGATCC 1 cut(s) 578
BanI GGYRCC 2 cut(s) 104, 127
BanII GRGCYC 3 cut(s) 298, 388, 675
Bbv12I GWGCWC 1 cut(s) 675
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 547
BccI CCATC 2 cut(s) 304, 638
BceAI ACGGC 1 cut(s) 538
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 1 cut(s) 2
BfmI CTRYAG 1 cut(s) 666
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 1 cut(s) 677
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 3 cut(s) 106, 129, 580
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 46
BmsI GCATC 2 cut(s) 87, 275
BmuI ACTGGG 1 cut(s) 46
Bpu10I CCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 619
BpuEI CTTGAG 2 cut(s) 203, 240
BpuMI CCSGG 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 14, 128
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 645
BsaXI ACNNNNNCTCC 3 cut(s) 29, 420, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 422
Bse118I RCCGGY 1 cut(s) 415
Bse1I ACTGG 2 cut(s) 41, 582
Bse3DI GCAATG 1 cut(s) 474
Bse8I GATNNNNATC 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 93
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 290, 422
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 14, 35
BseNI ACTGG 2 cut(s) 41, 582
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 1 cut(s) 547
BsgI GTGCAG 1 cut(s) 472
Bsh1236I CGCG 2 cut(s) 324, 575
BshFI GGCC 1 cut(s) 415
BshNI GGYRCC 2 cut(s) 104, 127
BsiHKAI GWGCWC 1 cut(s) 675
BsiSI CCGG 3 cut(s) 416, 646, 676
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 290, 422
BsmAI GTCTC 1 cut(s) 2
BsmBI CGTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 1 cut(s) 415
Bsp119I TTCGAA 1 cut(s) 619
Bsp1286I GDGCHC 3 cut(s) 298, 388, 675
Bsp143I GATC 3 cut(s) 400, 504, 578
Bsp19I CCATGG 1 cut(s) 93
Bsp68I TCGCGA 1 cut(s) 324
BspACI CCGC 3 cut(s) 5, 109, 575
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 2 cut(s) 15, 36
BspFNI CGCG 2 cut(s) 324, 575
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 3 cut(s) 106, 129, 580
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 3 cut(s) 395, 573, 586
BspQI GCTCTTC 1 cut(s) 50
BspT104I TTCGAA 1 cut(s) 619
BspT107I GGYRCC 2 cut(s) 104, 127
BsrDI GCAATG 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 415
BsrI ACTGG 2 cut(s) 41, 582
BssAI RCCGGY 1 cut(s) 415
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 3 cut(s) 400, 504, 578
BssNI GRCGYC 2 cut(s) 14, 128
BssT1I CCWWGG 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 2 cut(s) 14, 128
BstBI TTCGAA 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 417, 451
BstDEI CTNAG 3 cut(s) 23, 44, 508
BstDSI CCRYGG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 324, 575
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 403, 507, 581
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 3 cut(s) 400, 504, 578
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 666
BstUI CGCG 2 cut(s) 324, 575
BstV1I GCAGC 1 cut(s) 547
BstX2I RGATCY 2 cut(s) 400, 578
BstYI RGATCY 2 cut(s) 400, 578
BsuRI GGCC 1 cut(s) 415
BtgI CCRYGG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 2 cut(s) 588, 687
BtuMI TCGCGA 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 417, 451
CaiI CAGNNNCTG 1 cut(s) 36
CciI TCATGA 1 cut(s) 408
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 34, 105
CviAII CATG 4 cut(s) 94, 362, 409, 502
CviJI RGCY 7 cut(s) 158, 296, 352, 386, 415, 568, 673
CviKI_1 RGCY 7 cut(s) 158, 296, 352, 386, 415, 568, 673
CviQI GTAC 2 cut(s) 34, 105
DdeI CTNAG 3 cut(s) 23, 44, 508
DinI GGCGCC 1 cut(s) 129
DpnI GATC 3 cut(s) 402, 506, 580
DpnII GATC 3 cut(s) 400, 504, 578
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 3 cut(s) 298, 388, 675
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 673
EcoICRI GAGCTC 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 3 cut(s) 298, 388, 675
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 2
FaeI CATG 4 cut(s) 97, 365, 412, 505
FaiI YATR 9 cut(s) 95, 348, 363, 380, 382, 410, 479, 503, 688
FaqI GGGAC 1 cut(s) 182
FatI CATG 4 cut(s) 93, 361, 408, 501
FauI CCCGC 2 cut(s) 116, 568
Fnu4HI GCNGC 1 cut(s) 561
FriOI GRGCYC 3 cut(s) 298, 388, 675
Fsp4HI GCNGC 1 cut(s) 561
GlaI GCGC 1 cut(s) 129
GluI GCNGC 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 1 cut(s) 415
HapII CCGG 3 cut(s) 416, 646, 676
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 2 cut(s) 14, 128
Hin1II CATG 4 cut(s) 97, 365, 412, 505
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 482
HpaII CCGG 3 cut(s) 416, 646, 676
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 4 cut(s) 24, 66, 216, 249
Hpy188III TCNNGA 7 cut(s) 237, 274, 323, 374, 389, 409, 603
Hpy99I CGWCG 2 cut(s) 16, 19
HpyAV CCTTC 2 cut(s) 128, 351
HpyCH4IV ACGT 2 cut(s) 14, 195
HpyCH4V TGCA 7 cut(s) 266, 303, 453, 461, 501, 544, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 3 cut(s) 23, 44, 508
HpySE526I ACGT 2 cut(s) 14, 195
Hsp92I GRCGYC 2 cut(s) 14, 128
Hsp92II CATG 4 cut(s) 97, 365, 412, 505
HspAI GCGC 1 cut(s) 128
KasI GGCGCC 1 cut(s) 127
KpnI GGTACC 1 cut(s) 108
KroI GCCGGC 1 cut(s) 415
KroNI GCCGGC 1 cut(s) 417
Kzo9I GATC 3 cut(s) 400, 504, 578
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 1 cut(s) 678
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 2 cut(s) 87, 275
MaeII ACGT 2 cut(s) 14, 195
MaeIII GTNAC 4 cut(s) 357, 483, 518, 584
MalI GATC 3 cut(s) 402, 506, 580
MboI GATC 3 cut(s) 400, 504, 578
MboII GAAGA 3 cut(s) 67, 81, 311
MfeI CAATTG 1 cut(s) 122
MflI RGATCY 2 cut(s) 400, 578
MhlI GDGCHC 3 cut(s) 298, 388, 675
MluCI AATT 3 cut(s) 122, 488, 606
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 1 cut(s) 491
MnlI CCTC 9 cut(s) 18, 39, 68, 178, 283, 405, 415, 419, 636
MroNI GCCGGC 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 3 cut(s) 416, 646, 676
MspR9I CCNGG 1 cut(s) 677
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 60
MvnI CGCG 2 cut(s) 324, 575
MwoI GCNNNNNNNGC 1 cut(s) 155
NaeI GCCGGC 1 cut(s) 417
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 1 cut(s) 677
NcoI CCATGG 1 cut(s) 93
NdeII GATC 3 cut(s) 400, 504, 578
NgoMIV GCCGGC 1 cut(s) 415
NlaIII CATG 4 cut(s) 97, 365, 412, 505
NlaIV GGNNCC 3 cut(s) 106, 129, 580
NmuCI GTSAC 3 cut(s) 357, 483, 518
NruI TCGCGA 1 cut(s) 324
NspV TTCGAA 1 cut(s) 619
PagI TCATGA 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 50
PctI GAATGC 1 cut(s) 60
PdiI GCCGGC 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 318
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 562
PleI GAGTC 1 cut(s) 490
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 490
Psp124BI GAGCTC 1 cut(s) 675
PspN4I GGNNCC 3 cut(s) 106, 129, 580
PspPI GGNCC 1 cut(s) 338
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 36
PsuI RGATCY 2 cut(s) 400, 578
PsyI GACNNNGTC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 158
RruI TCGCGA 1 cut(s) 324
RsaI GTAC 2 cut(s) 35, 106
RsaNI GTAC 2 cut(s) 34, 105
SacI GAGCTC 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 50
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 3 cut(s) 400, 504, 578
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 3 cut(s) 298, 388, 675
SfaNI GCATC 2 cut(s) 87, 275
SfcI CTRYAG 1 cut(s) 666
SfoI GGCGCC 1 cut(s) 129
SfuI TTCGAA 1 cut(s) 619
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 182, 255
SmoI CTYRAG 2 cut(s) 182, 255
Sse9I AATT 3 cut(s) 122, 488, 606
SsiI CCGC 3 cut(s) 5, 109, 575
SspDI GGCGCC 1 cut(s) 127
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 1 cut(s) 93
TaiI ACGT 2 cut(s) 17, 198
TaqI TCGA 3 cut(s) 236, 438, 619
TasI AATT 3 cut(s) 122, 488, 606
TscAI CASTG 2 cut(s) 595, 687
TseFI GTSAC 3 cut(s) 357, 483, 518
TseI GCWGC 1 cut(s) 560
Tsp45I GTSAC 3 cut(s) 357, 483, 518
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 2 cut(s) 595, 687
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 318
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.