Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004810

Physical Location & Seq

Genomic Coordinates Reverse (-)

26292857 .. 26295612

Gene Structure 2756 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1134600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 996 bp

ATGATGACGAATAATTGCTTCCCAAATACTCATACTTGCAACACTTTGCTGCATAGCTTATGGAAGGAGGGAAGGACATCAGAAGCAGAGGAGCGACTGAAAAAGATGAATGAGAGAGGCTATGGCTTAGATACGGTGACCTGCAATATTGTGATTGATGGTCTATGCAACAATGGGAAATTGGACAAAGCAATTGAAATTGTGAGTGGGATGTGGACTCATGGAAGTGTGGCTCTTGGAAACATAGGGAACTCGTTTATTGGCCTGGTTGACAATAGTAATAATGGGAAGAAATGCATCCCTGATTTGATAACGTATTCAACCATAATTGGTGGGTTATGCAAGGCTGGGAGGCTTGATGAAGCGAAGAAGAAGTTCATGGAGATGTTGGGGAAAAACTTGCACCCTGATTCAGTGATTTATGATATGTTTATACAAAGTTTTTGTGAACAAGGAAAGATATCATCTGCATTTTGCGTGTTGAAGGACATGGAGAAAAATAGTTGCAACAAGAGCATTCGAACTTATAATTCACTGATCCTGGGATTAGGGAGGAAAAAGCAAATTTTTGAAATATATAGACTGATGGATGAGATGAGAGAAAGAGGATTCCCTCCTTATGTATGCACTTATAATAACATGATGAAAAGTCTCTGTGAAGGAGAGAGAGTCAGAGATGCCACTTCTCTTTTAGATGAAATCCTGCAGACAGGCATATCCCTGAATATATCTACCTTCAGAATATTGATTGAAGCTTTCGCCAAGGGATGTGATTTTGGAGCTGCGCATGAGGATGAAAAGTTAGAGGATGTTGCTAGCATCCTTCACGCAATGAAGAATAAAGAATACGGATTTGATCCAGCATCATTCCTGTCAGTAATTGATGGATTAGGTAAAAGGGGAAATAAGCAGGAGGCTGATGAACTTTCAGAGATAATGATGGAAATGGAGACCAAAGGTAGGGTAGCAGATAAGGTTTACCGAATTGAGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

332

Amino Acids

37.19

Weight (kDa)

6.14

Isoelectric Point (pI)

28.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	8 - 57	1.1e-11	PPR repeat family
PPR_1	PF12854	40 - 71	8.9e-08	PPR repeat
PPR_2	PF13041	44 - 71	5e-06	PPR repeat family
PPR	PF01535	46 - 75	8.9e-06	PPR repeat
PPR_1	PF12854	98 - 129	2.7e-12	PPR repeat
PPR_2	PF13041	101 - 139	9.4e-09	PPR repeat family
PPR	PF01535	104 - 133	3.5e-07	PPR repeat
PPR_1	PF12854	132 - 165	1.5e-06	PPR repeat
PPR_2	PF13041	136 - 184	4.1e-11	PPR repeat family
PPR_2	PF13041	172 - 219	3.4e-11	PPR repeat family
PPR_2	PF13041	207 - 255	2.5e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0004810)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17140 AT2G17140
fragaria_vesca	FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640
malus_domestica	MD05G1134600.v1.1 MD05G1134700.v1.1 MD10G1139700.v1.1
prunus_persica	Prupe.8G178500_v2.0.a1 Prupe.8G178500_v2.0.a1
pyrus_communis	pycom05g13140 pycom10g11860
rosa_chinensis	RchiOBHm_Chr6g0280761
rosa_laevigata	RLG00000013030
rosa_multiflora	Rmu_co8435227.1_g000001 Rmu_sc0042093.1_g000001 Rmu_sc0042110.1_g000001
rosa_roxburghii	Rroxscaffold_1G00044300 Rroxscaffold_6G00391320 Rroxscaffold_6G00391330 Rroxscaffold_7G00187700
rosa_rugosa	Rorug06G0134300
rosa_samantha	Rh6AG247000 Rh6BG249100 Rh6CG250400 Rh6DG241800
rosa_wichuraiana	Rw6G021390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	528, 633
Acc16I	TGCGCA	1 cut(s)	786
Acc36I	ACCTGC	1 cut(s)	149
AclWI	GGATC	2 cut(s)	532, 851
AcsI	RAATTY	1 cut(s)	564
AcuI	CTGAAG	1 cut(s)	721
AfiI	CCNNNNNNNGG	1 cut(s)	960
AgsI	TTSAA	5 cut(s)	197, 321, 484, 572, 752
AjnI	CCWGG	2 cut(s)	264, 540
AluBI	AGCT	3 cut(s)	57, 755, 782
AluI	AGCT	3 cut(s)	57, 755, 782
Alw26I	GTCTC	2 cut(s)	656, 944
AlwI	GGATC	2 cut(s)	532, 851
AoxI	GGCC	1 cut(s)	262
ApeKI	GCWGC	2 cut(s)	49, 782
ApoI	RAATTY	1 cut(s)	564
Asp700I	GAANNNNTTC	1 cut(s)	374
AspLEI	GCGC	1 cut(s)	787
AsuHPI	GGTGA	1 cut(s)	148
AsuII	TTCGAA	1 cut(s)	520
AsuNHI	GCTAGC	1 cut(s)	815
BbvI	GCAGC	2 cut(s)	36, 769
BccI	CCATC	4 cut(s)	152, 580, 878, 934
BciT130I	CCWGG	2 cut(s)	266, 542
BcoDI	GTCTC	2 cut(s)	656, 944
BfaI	CTAG	1 cut(s)	816
BfmI	CTRYAG	1 cut(s)	704
BfuAI	ACCTGC	1 cut(s)	149
BisI	GCNGC	2 cut(s)	50, 783
BlsI	GCNGC	2 cut(s)	51, 784
Bme1390I	CCNGG	2 cut(s)	266, 542
BmrFI	CCNGG	2 cut(s)	266, 542
BmsI	GCATC	4 cut(s)	306, 667, 828, 872
BmtI	GCTAGC	1 cut(s)	819
Bpu14I	TTCGAA	1 cut(s)	520
BsaI	GGTCTC	1 cut(s)	944
BsaJI	CCNNGG	2 cut(s)	541, 762
BsaXI	ACNNNNNCTCC	2 cut(s)	654, 684
Bsc4I	CCNNNNNNNGG	1 cut(s)	960
Bse3DI	GCAATG	1 cut(s)	837
BseBI	CCWGG	2 cut(s)	266, 542
BseDI	CCNNGG	2 cut(s)	541, 762
BseGI	GGATG	7 cut(s)	216, 297, 595, 773, 799, 814, 819
BseLI	CCNNNNNNNGG	1 cut(s)	960
BseMI	GCAATG	1 cut(s)	837
BseRI	GAGGAG	1 cut(s)	104
BseXI	GCAGC	2 cut(s)	36, 769
BseYI	CCCAGC	1 cut(s)	347
BshFI	GGCC	1 cut(s)	264
BslI	CCNNNNNNNGG	1 cut(s)	960
BsmAI	GTCTC	2 cut(s)	656, 944
BsmI	GAATGC	1 cut(s)	516
BsnI	GGCC	1 cut(s)	264
Bso31I	GGTCTC	1 cut(s)	944
Bsp119I	TTCGAA	1 cut(s)	520
Bsp143I	GATC	2 cut(s)	537, 856
BspANI	GGCC	1 cut(s)	264
BspMAI	CTGCAG	1 cut(s)	708
BspMI	ACCTGC	1 cut(s)	149
BspOI	GCTAGC	1 cut(s)	819
BspPI	GGATC	2 cut(s)	532, 851
BspT104I	TTCGAA	1 cut(s)	520
BspTNI	GGTCTC	1 cut(s)	944
BsrDI	GCAATG	1 cut(s)	837
BssECI	CCNNGG	2 cut(s)	541, 762
BssMI	GATC	2 cut(s)	537, 856
BssT1I	CCWWGG	1 cut(s)	762
Bst2UI	CCWGG	2 cut(s)	266, 542
Bst4CI	ACNGT	1 cut(s)	136
BstBI	TTCGAA	1 cut(s)	520
BstC8I	GCNNGC	1 cut(s)	817
BstDEI	CTNAG	1 cut(s)	127
BstEII	GGTNACC	1 cut(s)	136
BstF5I	GGATG	7 cut(s)	216, 297, 595, 773, 799, 814, 819
BstHHI	GCGC	1 cut(s)	787
BstKTI	GATC	2 cut(s)	540, 859
BstMAI	GTCTC	2 cut(s)	656, 944
BstMBI	GATC	2 cut(s)	537, 856
BstMWI	GCNNNNNNNGC	1 cut(s)	513
BstNI	CCWGG	2 cut(s)	266, 542
BstPI	GGTNACC	1 cut(s)	136
BstSCI	CCNGG	2 cut(s)	264, 540
BstSFI	CTRYAG	1 cut(s)	704
BstV1I	GCAGC	2 cut(s)	36, 769
BsuRI	GGCC	1 cut(s)	264
BtsCI	GGATG	7 cut(s)	216, 297, 595, 773, 799, 814, 819
BtsIMutI	CAGTG	2 cut(s)	420, 533
BveI	ACCTGC	1 cut(s)	149
Cac8I	GCNNGC	1 cut(s)	817
CfoI	GCGC	1 cut(s)	787
CviAII	CATG	5 cut(s)	221, 379, 490, 640, 788
DdeI	CTNAG	1 cut(s)	127
DpnI	GATC	2 cut(s)	539, 858
DpnII	GATC	2 cut(s)	537, 856
Eco130I	CCWWGG	1 cut(s)	762
Eco31I	GGTCTC	1 cut(s)	944
Eco32I	GATATC	1 cut(s)	462
Eco57I	CTGAAG	1 cut(s)	721
Eco91I	GGTNACC	1 cut(s)	136
EcoO65I	GGTNACC	1 cut(s)	136
EcoRII	CCWGG	2 cut(s)	264, 540
EcoRV	GATATC	1 cut(s)	462
EcoT14I	CCWWGG	1 cut(s)	762
EcoT22I	ATGCAT	1 cut(s)	299
ErhI	CCWWGG	1 cut(s)	762
FaeI	CATG	5 cut(s)	224, 382, 493, 643, 791
FatI	CATG	5 cut(s)	220, 378, 489, 639, 787
Fnu4HI	GCNGC	2 cut(s)	50, 783
FokI	GGATG	7 cut(s)	223, 284, 602, 780, 806, 806, 821
Fsp4HI	GCNGC	2 cut(s)	50, 783
FspBI	CTAG	1 cut(s)	816
FspI	TGCGCA	1 cut(s)	786
GlaI	GCGC	1 cut(s)	786
GluI	GCNGC	2 cut(s)	50, 783
GsaI	CCCAGC	1 cut(s)	351
HaeIII	GGCC	1 cut(s)	264
HhaI	GCGC	1 cut(s)	787
Hin1II	CATG	5 cut(s)	224, 382, 493, 643, 791
Hin6I	GCGC	1 cut(s)	785
HinP1I	GCGC	1 cut(s)	785
HincII	GTYRAC	1 cut(s)	271
HindII	GTYRAC	1 cut(s)	271
HindIII	AAGCTT	1 cut(s)	753
HinfI	GANTC	4 cut(s)	217, 410, 609, 669
HphI	GGTGA	1 cut(s)	148
Hpy166II	GTNNAC	4 cut(s)	216, 271, 449, 979
Hpy188I	TCNGA	4 cut(s)	82, 674, 740, 931
Hpy8I	GTNNAC	4 cut(s)	216, 271, 449, 979
HpyAV	CCTTC	6 cut(s)	58, 66, 478, 653, 745, 833
HpyCH4III	ACNGT	1 cut(s)	136
HpyCH4IV	ACGT	1 cut(s)	314
HpyF10VI	GCNNNNNNNGC	1 cut(s)	513
HpyF3I	CTNAG	1 cut(s)	127
HpySE526I	ACGT	1 cut(s)	314
Hsp92II	CATG	5 cut(s)	224, 382, 493, 643, 791
HspAI	GCGC	1 cut(s)	785
Kzo9I	GATC	2 cut(s)	537, 856
LmnI	GCTCC	2 cut(s)	91, 779
Lsp1109I	GCAGC	2 cut(s)	36, 769
LweI	GCATC	4 cut(s)	306, 667, 828, 872
MaeI	CTAG	1 cut(s)	816
MaeII	ACGT	1 cut(s)	314
MaeIII	GTNAC	1 cut(s)	136
MalI	GATC	2 cut(s)	539, 858
MboI	GATC	2 cut(s)	537, 856
MboII	GAAGA	4 cut(s)	301, 379, 382, 847
MfeI	CAATTG	1 cut(s)	192
MluCI	AATT	9 cut(s)	13, 179, 192, 198, 327, 529, 564, 879, 984
MlyI	GAGTC	2 cut(s)	211, 678
Mph1103I	ATGCAT	1 cut(s)	299
MroXI	GAANNNNTTC	1 cut(s)	374
MslI	CAYNNNNRTG	3 cut(s)	225, 383, 792
MspR9I	CCNGG	2 cut(s)	266, 542
MunI	CAATTG	1 cut(s)	192
Mva1269I	GAATGC	1 cut(s)	516
MvaI	CCWGG	2 cut(s)	266, 542
MwoI	GCNNNNNNNGC	1 cut(s)	513
NdeII	GATC	2 cut(s)	537, 856
NheI	GCTAGC	1 cut(s)	815
NlaIII	CATG	5 cut(s)	224, 382, 493, 643, 791
NmuCI	GTSAC	1 cut(s)	136
NsbI	TGCGCA	1 cut(s)	786
NsiI	ATGCAT	1 cut(s)	299
NspV	TTCGAA	1 cut(s)	520
PctI	GAATGC	1 cut(s)	516
PdmI	GAANNNNTTC	1 cut(s)	374
PfeI	GAWTC	2 cut(s)	410, 609
PkrI	GCNGC	2 cut(s)	51, 784
PleI	GAGTC	2 cut(s)	211, 677
PpsI	GAGTC	2 cut(s)	211, 677
PsiI	TTATAA	2 cut(s)	528, 633
Psp6I	CCWGG	2 cut(s)	264, 540
PspEI	GGTNACC	1 cut(s)	136
PspFI	CCCAGC	1 cut(s)	347
PspGI	CCWGG	2 cut(s)	264, 540
PstI	CTGCAG	1 cut(s)	708
RseI	CAYNNNNRTG	3 cut(s)	225, 383, 792
SatI	GCNGC	2 cut(s)	50, 783
Sau3AI	GATC	2 cut(s)	537, 856
SchI	GAGTC	2 cut(s)	211, 678
ScrFI	CCNGG	2 cut(s)	266, 542
SfaNI	GCATC	4 cut(s)	306, 667, 828, 872
SfcI	CTRYAG	1 cut(s)	704
SfuI	TTCGAA	1 cut(s)	520
SmiMI	CAYNNNNRTG	3 cut(s)	225, 383, 792
Sse9I	AATT	9 cut(s)	13, 179, 192, 198, 327, 529, 564, 879, 984
SspI	AATATT	2 cut(s)	148, 744
SspMI	CTAG	1 cut(s)	816
StyD4I	CCNGG	2 cut(s)	264, 540
StyI	CCWWGG	1 cut(s)	762
TaaI	ACNGT	1 cut(s)	136
TaiI	ACGT	1 cut(s)	317
TaqI	TCGA	1 cut(s)	520
TasI	AATT	9 cut(s)	13, 179, 192, 198, 327, 529, 564, 879, 984
TfiI	GAWTC	2 cut(s)	410, 609
TscAI	CASTG	2 cut(s)	420, 540
TseFI	GTSAC	1 cut(s)	136
TseI	GCWGC	2 cut(s)	49, 782
Tsp45I	GTSAC	1 cut(s)	136
TspDTI	ATGAA	8 cut(s)	122, 367, 375, 659, 711, 810, 848, 936
TspGWI	ACGGA	1 cut(s)	864
TspRI	CASTG	2 cut(s)	420, 540
XapI	RAATTY	1 cut(s)	564
XmnI	GAANNNNTTC	1 cut(s)	374
XspI	CTAG	1 cut(s)	816
Zsp2I	ATGCAT	1 cut(s)	299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.