Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0004810

Physical Location & Seq

Genomic Coordinates Reverse (-)

46083731 .. 46095920

Gene Structure 12190 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG249100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2571 bp

ATGGAGGAAGCTCTACTCAACAACACCAACAATCCCAAACTCGCATGGCACCTCTTCAAGCGCATTCTCTCCTCCTCCTCACATCTCTCCCTCCGCTCCCTCACCATCATAGCCCGCATCCTCATCTCCGCCAAAATGCACCCCGAAATCGACTCCCTCCACCACCTCCTCCTCCACTCACAGCCCCCCCAAACCCTCCGCCCCTGCCTCGTTTCCCTCGTGCGAATCTTCGCCAAGTCACGCCTCACCGAAAAGACCCTTTCGCATTTTAAGTCCCTCCAAAGCCACTTCCCCAATGACCCTCCTTCTGTTTATTTGTATAATCTGCTTCTCGAGTCCGCTCTCAGGGAGAACGATGTGGATTACGTGCTGTGGTTGTACAAGGATATGATTGTTTCCGGGGTTAAGCCCGAAACGTATACTTTTAATCTTCTGATCTGTGCTCTGTGCGATTGTGGTCGTTTGGAAGACGCACGCCAGGTGTTCGATAAAATGCGTGACAAGGGTTGTCGGCCGAATGAGTACAGCGTTGGGATTTTGGTTCGCGGGTATTGCAGAGCTGGTTTGAGCAGTGGAGGGTTGGAGGTTTTGGATTGTGGTGTTGTGCCCAATAGGGTTGTGTATAATACTTTGGTTTCGAGTTTTTGTAAAGAAGGCAGGACTGATGAGGCCGAGAAATTGGTGGAGAGGATGCGAGAGGAGGGTATGCTTCCGGATGTCATTACTTTCAATTCGAGGATATCGGCTCTCTGTAGTGCAGGGAAGATTCTTGAAGCGTCTAGAATATTTAGAGATATGCATACGGATCAGGCTTTGGGGCTGCCGCAACCCAATGTTGTAACGTATAATCTGATGTTACAAGGATTTTGCAAGGAAGGGATGTTAGAGGAAGCAGAGAGCTTGTTCAAATCTATGGAAAAAGCTGGTGGTTTAATTAATGTAGAGAGCTATAATATATGGTTGTTGGGTTTGGTCAGGAATAAGAAGCTCTTGGAGGCTCGACTGGTTCTGAAAGAGATGGTAGATAAGGGAATAGAACCCAACATTTACTCATATAACATAGTGATAAATGGGTTATGCAAAAATGGGATGCTCCGTGATGCAAGAATTGTGATGGGTCTGATGGCAAGAAACAATATTTCGCCAGATACGGTTACCTACAGTACTTTACTACACGGATACTGCAATAAAGGGAAGGTATTTGAAGTGAACAATGTTCTGCAAGAGATGATAACAAAGAATTGCTTCCCAAATACTCATACTTGCAACATTCTGCTGCACAGCCTATGGAAAGAGGGAAGAACATCAGAAGCGGAGGAACTGCTGCGAAAGATGAATGAGAGAGGTTATGGTTTAGATACTGTGACCTGCAACATCGTGATCGATGGTCTGTGCAATGATGGGAAATTGGACAAAGCAATTGAAATTGTAAGTGGGATGTGGACTCATGGAAGTGCGGCTCTTGGAAACTTAGGGAATTCCTTTGTTGGCCTGGTTGATGATAGTAATAATGGGAATAAATGCCTTCCAGATTTGATCTCATATTCAACCATAATTAGTGGGTTATGCAAGGCTGGGAGGCTTGATGAAGCTAAGAAGAAGTTCATGGAGATGATGGGCAAAAACTTGCACCCTGATTCAGTGATTTATGATACTTTTATACATAGCTTTTGTAAACAAGGAAGGATATCATCTGCATTTCGGGTGTTGAAGGACATGGAGAAGAAAGGTTGCAACAAGAGCATTCAAACTTATAATTCACTAATCCTGGGCTTAGGGAGTAAAAAGCAAATATTTGAAATATATGGGCTGATGGATGAAATGAAAGAGAGGGGAGTCCCTCCTGATGTCTGTACTTACAATAATATGATGTACTGTCTCTGTGAAGTAGAGAGAGTCAAAGATGCAACTTCTCTTTTAGATGAAATGCTGCAAAAGGGCATATCCCCGAATATTTCTACCTTCAGAATATTAATTAAAGCTTTCTGCAAGGGTTGTGATTTTGGAGTCGCACAGGAGTTATTTGACATTGCTTTAAGTGTATGTGGCCACAAGGAAGCCTTATATAGTTTGATGTTTAATGAGTTACTTGCTGGAGGGGAAGTCACAAAAGCTACAGAGCTATTTGAAGAGGCATTGGACAAGTATTTTTATCTGGGGAACTTCCTTTACAAAGATCTCATTGATAGACTTTGCAAGGATCAAAAGTTAGAGGATGCCTGTAGCATTCTTCACAATATGATGAATAAGGGATACGGGTTTGATTCAGCATCCTTCCTACCAGTAATTGATGGCTTGGGTAAAAGGGGGAATAAGCATGAGGCTGATGAACTTGCAGAGAGAATGATGGAAATGGCATCAGAAGGTAGGATAGCAAATAAGGTTTACCGAAATGAGAGGGATATAATTGGTGGAAAACCAAAGTCTCGTGGAAGTGATTGGCAGACCATAGTGAACAGAGATGATGGCAGTGGAGTTGTATTGAAAACTCTTAAGAGGGTGCAGAAAGGCTGGGGTCGAGCGAATCTAACGAGTTTGCAAACCCAGAAAGATGAATTTCTTGATTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

856

Amino Acids

96.3

Weight (kDa)

7.19

Isoelectric Point (pI)

36.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	102 - 151	6.5e-09	PPR repeat family
PPR_3	PF13812	127 - 173	4.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	134 - 166	1.6e-10	PPR repeat
PPR_2	PF13041	137 - 186	4.7e-15	PPR repeat family
PPR	PF01535	140 - 170	1.7e-07	PPR repeat
PPR_3	PF13812	199 - 249	6.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	200 - 232	3.9e-13	PPR repeat
PPR_2	PF13041	203 - 251	1.1e-13	PPR repeat family
PPR	PF01535	206 - 235	1.1e-08	PPR repeat
PPR_1	PF12854	235 - 266	6.3e-08	PPR repeat
PPR_1	PF12854	276 - 306	2.3e-11	PPR repeat
PPR_2	PF13041	277 - 320	8.3e-13	PPR repeat family
PPR	PF01535	280 - 309	7.1e-10	PPR repeat
PPR_2	PF13041	313 - 361	1.4e-13	PPR repeat family
PPR_1	PF12854	343 - 368	2.8e-09	PPR repeat
PPR_2	PF13041	347 - 396	6.5e-16	PPR repeat family
TPR_24	PF23276	371 - 476	1.7e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	375 - 427	1.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	379 - 410	1.8e-08	PPR repeat
PPR_2	PF13041	382 - 429	1.6e-13	PPR repeat family
PPR	PF01535	385 - 415	4.4e-06	PPR repeat
PPR_2	PF13041	417 - 466	6e-12	PPR repeat family
PPR_1	PF12854	449 - 480	6.1e-07	PPR repeat
PPR_1	PF12854	508 - 538	7.3e-12	PPR repeat
PPR_2	PF13041	510 - 548	6.4e-08	PPR repeat family
PPR	PF01535	513 - 542	9.1e-07	PPR repeat
PPR_3	PF13812	536 - 589	1.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	541 - 574	3.7e-10	PPR repeat
PPR_2	PF13041	545 - 592	2.6e-13	PPR repeat family
PPR_long	PF17177	562 - 663	2.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	581 - 628	5e-12	PPR repeat family
PPR_3	PF13812	604 - 662	5.9e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	611 - 643	4.9e-08	PPR repeat
PPR_2	PF13041	615 - 664	1.6e-16	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004810)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17140 AT2G17140
fragaria_vesca	FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640
malus_domestica	MD05G1134600.v1.1 MD05G1134700.v1.1 MD10G1139700.v1.1
prunus_persica	Prupe.8G178500_v2.0.a1 Prupe.8G178500_v2.0.a1
pyrus_communis	pycom05g13140 pycom10g11860
rosa_chinensis	RchiOBHm_Chr6g0280761
rosa_laevigata	RLG00000013030
rosa_multiflora	Rmu_co8435227.1_g000001 Rmu_sc0042093.1_g000001 Rmu_sc0042110.1_g000001
rosa_roxburghii	Rroxscaffold_1G00044300 Rroxscaffold_6G00391320 Rroxscaffold_6G00391330 Rroxscaffold_7G00187700
rosa_rugosa	Rorug06G0134300
rosa_samantha	Rh6AG247000 Rh6BG249100 Rh6CG250400 Rh6DG241800
rosa_wichuraiana	Rw6G021390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1755
Acc36I	ACCTGC	1 cut(s)	1376
AccB1I	GGYRCC	1 cut(s)	48
AccBSI	CCGCTC	2 cut(s)	96, 341
AccI	GTMKAC	1 cut(s)	419
AccII	CGCG	1 cut(s)	546
AccIII	TCCGGA	1 cut(s)	712
AciI	CCGC	9 cut(s)	94, 115, 129, 199, 339, 546, 824, 1313, 1457
AclWI	GGATC	2 cut(s)	813, 2208
AcoI	YGGCCR	2 cut(s)	512, 2047
AcsI	RAATTY	2 cut(s)	1477, 2555
AcuI	CTGAAG	1 cut(s)	1948
AfaI	GTAC	5 cut(s)	380, 524, 1165, 1855, 1874
AfiI	CCNNNNNNNGG	1 cut(s)	345
AflII	CTTAAG	1 cut(s)	2492
AjnI	CCWGG	3 cut(s)	477, 1491, 1767
Alw21I	GWGCWC	1 cut(s)	445
Alw26I	GTCTC	2 cut(s)	1883, 2430
AlwI	GGATC	2 cut(s)	813, 2208
Ama87I	CYCGRG	1 cut(s)	332
Aor13HI	TCCGGA	1 cut(s)	712
AoxI	GGCC	4 cut(s)	512, 669, 1489, 2047
ApeKI	GCWGC	4 cut(s)	820, 1276, 1324, 1930
ApoI	RAATTY	2 cut(s)	1477, 2555
AseI	ATTAAT	2 cut(s)	936, 1973
Asp700I	GAANNNNTTC	2 cut(s)	1244, 1601
AspLEI	GCGC	1 cut(s)	63
AsuC2I	CCSGG	1 cut(s)	400
AsuHPI	GGTGA	2 cut(s)	94, 238
AvaI	CYCGRG	1 cut(s)	332
BaeGI	GKGCMC	1 cut(s)	609
BalI	TGGCCA	1 cut(s)	2049
BanI	GGYRCC	1 cut(s)	48
BauI	CACGAG	2 cut(s)	218, 2427
BbsI	GAAGAC	1 cut(s)	474
Bbv12I	GWGCWC	1 cut(s)	445
BbvI	GCAGC	4 cut(s)	807, 1263, 1311, 1917
BciT130I	CCWGG	3 cut(s)	479, 1493, 1769
BciVI	GTATCC	2 cut(s)	1172, 2246
BcnI	CCSGG	1 cut(s)	400
BcoDI	GTCTC	2 cut(s)	1883, 2430
BfaI	CTAG	1 cut(s)	780
BfmI	CTRYAG	4 cut(s)	751, 1159, 2115, 2221
BfrI	CTTAAG	1 cut(s)	2492
BfuAI	ACCTGC	1 cut(s)	1376
BfuI	GTATCC	2 cut(s)	1172, 2246
BglII	AGATCT	1 cut(s)	2176
BisI	GCNGC	6 cut(s)	821, 824, 1277, 1325, 1458, 1931
BlsI	GCNGC	6 cut(s)	822, 825, 1278, 1326, 1459, 1932
BmcAI	AGTACT	1 cut(s)	1165
Bme1390I	CCNGG	4 cut(s)	400, 479, 1493, 1769
BmeT110I	CYCGRG	1 cut(s)	332
BmiI	GGNNCC	1 cut(s)	50
BmrFI	CCNGG	4 cut(s)	400, 479, 1493, 1769
BmsI	GCATC	8 cut(s)	126, 681, 1080, 1090, 1894, 2206, 2279, 2366
BpiI	GAAGAC	1 cut(s)	474
BpmI	CTGGAG	1 cut(s)	2115
Bpu10I	CCTNAGC	1 cut(s)	1774
BpuMI	CCSGG	1 cut(s)	400
Bsa29I	ATCGAT	1 cut(s)	1383
BsaAI	YACGTR	1 cut(s)	367
BsaJI	CCNNGG	2 cut(s)	399, 1768
BsaWI	WCCGGW	1 cut(s)	712
BsaXI	ACNNNNNCTCC	2 cut(s)	2088, 2118
Bsc4I	CCNNNNNNNGG	1 cut(s)	345
Bse1I	ACTGG	2 cut(s)	1008, 2282
Bse3DI	GCAATG	2 cut(s)	1402, 2028
BseAI	TCCGGA	1 cut(s)	712
BseBI	CCWGG	3 cut(s)	479, 1493, 1769
BseCI	ATCGAT	1 cut(s)	1383
BseDI	CCNNGG	2 cut(s)	399, 1768
BseGI	GGATG	9 cut(s)	117, 696, 721, 885, 1095, 1443, 1822, 2221, 2270
BseLI	CCNNNNNNNGG	1 cut(s)	345
BseMI	GCAATG	2 cut(s)	1402, 2028
BseMII	CTCAG	1 cut(s)	358
BseNI	ACTGG	2 cut(s)	1008, 2282
BseRI	GAGGAG	6 cut(s)	61, 64, 67, 158, 161, 713
BseSI	GKGCMC	1 cut(s)	609
BseX3I	CGGCCG	1 cut(s)	512
BseXI	GCAGC	4 cut(s)	807, 1263, 1311, 1917
BseYI	CCCAGC	2 cut(s)	1574, 2511
BsgI	GTGCAG	3 cut(s)	777, 1262, 2522
Bsh1236I	CGCG	1 cut(s)	546
Bsh1285I	CGRYCG	1 cut(s)	515
BshFI	GGCC	4 cut(s)	514, 671, 1491, 2049
BshNI	GGYRCC	1 cut(s)	48
BshVI	ATCGAT	1 cut(s)	1383
BsiEI	CGRYCG	1 cut(s)	515
BsiHKAI	GWGCWC	1 cut(s)	445
BsiHKCI	CYCGRG	1 cut(s)	332
BsiSI	CCGG	2 cut(s)	399, 713
BslFI	GGGAC	2 cut(s)	259, 1823
BslI	CCNNNNNNNGG	1 cut(s)	345
BsmAI	GTCTC	2 cut(s)	1883, 2430
BsmFI	GGGAC	2 cut(s)	259, 1823
BsmI	GAATGC	3 cut(s)	63, 1743, 2226
BsnI	GGCC	4 cut(s)	514, 671, 1491, 2049
BsoBI	CYCGRG	1 cut(s)	332
Bsp1286I	GDGCHC	2 cut(s)	445, 609
Bsp13I	TCCGGA	1 cut(s)	712
Bsp1407I	TGTACA	1 cut(s)	378
Bsp143I	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
BspACI	CCGC	9 cut(s)	94, 115, 129, 199, 339, 546, 824, 1313, 1457
BspANI	GGCC	4 cut(s)	514, 671, 1491, 2049
BspCNI	CTCAG	1 cut(s)	357
BspDI	ATCGAT	1 cut(s)	1383
BspEI	TCCGGA	1 cut(s)	712
BspFNI	CGCG	1 cut(s)	546
BspLI	GGNNCC	1 cut(s)	50
BspMI	ACCTGC	1 cut(s)	1376
BspPI	GGATC	2 cut(s)	813, 2208
BspT107I	GGYRCC	1 cut(s)	48
BspTI	CTTAAG	1 cut(s)	2492
BsrBI	CCGCTC	2 cut(s)	96, 341
BsrDI	GCAATG	2 cut(s)	1402, 2028
BsrGI	TGTACA	1 cut(s)	378
BsrI	ACTGG	2 cut(s)	1008, 2282
BssECI	CCNNGG	2 cut(s)	399, 1768
BssMI	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
BssNAI	GTATAC	1 cut(s)	420
BssSI	CACGAG	2 cut(s)	218, 2427
Bst1107I	GTATAC	1 cut(s)	420
Bst2BI	CACGAG	2 cut(s)	218, 2427
Bst2UI	CCWGG	3 cut(s)	479, 1493, 1769
Bst4CI	ACNGT	4 cut(s)	1153, 1163, 1363, 1877
Bst6I	CTCTTC	2 cut(s)	59, 2124
BstAFI	CTTAAG	1 cut(s)	2492
BstAUI	TGTACA	1 cut(s)	378
BstBAI	YACGTR	1 cut(s)	367
BstC8I	GCNNGC	2 cut(s)	115, 475
BstDEI	CTNAG	4 cut(s)	344, 1471, 1593, 1774
BstEII	GGTNACC	1 cut(s)	1153
BstF5I	GGATG	9 cut(s)	117, 696, 721, 885, 1095, 1443, 1822, 2221, 2270
BstFNI	CGCG	1 cut(s)	546
BstHHI	GCGC	1 cut(s)	63
BstKTI	GATC	6 cut(s)	438, 808, 1383, 1539, 2179, 2203
BstMAI	GTCTC	2 cut(s)	1883, 2430
BstMBI	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
BstMCI	CGRYCG	1 cut(s)	515
BstMWI	GCNNNNNNNGC	2 cut(s)	552, 1740
BstNI	CCWGG	3 cut(s)	479, 1493, 1769
BstPI	GGTNACC	1 cut(s)	1153
BstSCI	CCNGG	4 cut(s)	398, 477, 1491, 1767
BstSFI	CTRYAG	4 cut(s)	751, 1159, 2115, 2221
BstSLI	GKGCMC	1 cut(s)	609
BstUI	CGCG	1 cut(s)	546
BstV1I	GCAGC	4 cut(s)	807, 1263, 1311, 1917
BstV2I	GAAGAC	1 cut(s)	474
BstX2I	RGATCY	1 cut(s)	2176
BstYI	RGATCY	1 cut(s)	2176
BstZ17I	GTATAC	1 cut(s)	420
BstZI	CGGCCG	1 cut(s)	512
Bsu15I	ATCGAT	1 cut(s)	1383
BsuI	GTATCC	2 cut(s)	1172, 2246
BsuRI	GGCC	4 cut(s)	514, 671, 1491, 2049
BsuTUI	ATCGAT	1 cut(s)	1383
BtsCI	GGATG	9 cut(s)	117, 696, 721, 885, 1095, 1443, 1822, 2221, 2270
BtsI	GCAGTG	2 cut(s)	577, 2476
BtsIMutI	CAGTG	3 cut(s)	577, 1647, 2476
BveI	ACCTGC	1 cut(s)	1376
Cac8I	GCNNGC	2 cut(s)	115, 475
CfoI	GCGC	1 cut(s)	63
ClaI	ATCGAT	1 cut(s)	1383
CseI	GACGC	2 cut(s)	479, 765
Csp6I	GTAC	5 cut(s)	379, 523, 1164, 1854, 1873
CviAII	CATG	5 cut(s)	45, 1448, 1606, 1717, 2318
CviQI	GTAC	5 cut(s)	379, 523, 1164, 1854, 1873
DdeI	CTNAG	4 cut(s)	344, 1471, 1593, 1774
DpnI	GATC	6 cut(s)	437, 807, 1382, 1538, 2178, 2202
DpnII	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
EaeI	YGGCCR	2 cut(s)	512, 2047
EagI	CGGCCG	1 cut(s)	512
Eam1104I	CTCTTC	2 cut(s)	59, 2124
EarI	CTCTTC	2 cut(s)	59, 2124
EciI	GGCGGA	2 cut(s)	118, 188
EclXI	CGGCCG	1 cut(s)	512
Eco32I	GATATC	2 cut(s)	741, 1689
Eco52I	CGGCCG	1 cut(s)	512
Eco57I	CTGAAG	1 cut(s)	1948
Eco88I	CYCGRG	1 cut(s)	332
Eco91I	GGTNACC	1 cut(s)	1153
EcoO65I	GGTNACC	1 cut(s)	1153
EcoRI	GAATTC	1 cut(s)	1477
EcoRII	CCWGG	3 cut(s)	477, 1491, 1767
EcoRV	GATATC	2 cut(s)	741, 1689
EcoT22I	ATGCAT	1 cut(s)	801
FaeI	CATG	5 cut(s)	48, 1451, 1609, 1720, 2321
FalI	AAGNNNNNCTT	6 cut(s)	974, 1006, 1229, 1261, 2045, 2077
FaqI	GGGAC	2 cut(s)	259, 1823
FatI	CATG	5 cut(s)	44, 1447, 1605, 1716, 2317
FauI	CCCGC	2 cut(s)	122, 539
FblI	GTMKAC	1 cut(s)	419
Fnu4HI	GCNGC	6 cut(s)	821, 824, 1277, 1325, 1458, 1931
FokI	GGATG	9 cut(s)	104, 703, 728, 892, 1102, 1450, 1829, 2228, 2257
Fsp4HI	GCNGC	6 cut(s)	821, 824, 1277, 1325, 1458, 1931
FspBI	CTAG	1 cut(s)	780
GlaI	GCGC	1 cut(s)	62
GluI	GCNGC	6 cut(s)	821, 824, 1277, 1325, 1458, 1931
GsaI	CCCAGC	2 cut(s)	1578, 2515
GsuI	CTGGAG	1 cut(s)	2115
HaeIII	GGCC	4 cut(s)	514, 671, 1491, 2049
HapII	CCGG	2 cut(s)	399, 713
HgaI	GACGC	2 cut(s)	479, 765
HhaI	GCGC	1 cut(s)	63
Hin1II	CATG	5 cut(s)	48, 1451, 1609, 1720, 2321
Hin6I	GCGC	1 cut(s)	61
HinP1I	GCGC	1 cut(s)	61
HindIII	AAGCTT	1 cut(s)	1980
HpaII	CCGG	2 cut(s)	399, 713
HphI	GGTGA	2 cut(s)	94, 238
Hpy166II	GTNNAC	6 cut(s)	420, 1210, 1443, 1676, 2386, 2455
Hpy188I	TCNGA	7 cut(s)	435, 852, 1011, 1122, 1309, 1967, 2362
Hpy188III	TCNNGA	9 cut(s)	332, 713, 770, 780, 976, 1378, 1529, 1844, 2561
Hpy8I	GTNNAC	6 cut(s)	420, 1210, 1443, 1676, 2386, 2455
HpyCH4III	ACNGT	4 cut(s)	1153, 1163, 1363, 1877
HpyCH4IV	ACGT	3 cut(s)	366, 416, 842
HpyF10VI	GCNNNNNNNGC	2 cut(s)	552, 1740
HpyF3I	CTNAG	4 cut(s)	344, 1471, 1593, 1774
HpySE526I	ACGT	3 cut(s)	366, 416, 842
Hsp92II	CATG	5 cut(s)	48, 1451, 1609, 1720, 2321
HspAI	GCGC	1 cut(s)	61
Kpn2I	TCCGGA	1 cut(s)	712
Kzo9I	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
LmnI	GCTCC	2 cut(s)	101, 1098
Lsp1109I	GCAGC	4 cut(s)	807, 1263, 1311, 1917
LweI	GCATC	8 cut(s)	126, 681, 1080, 1090, 1894, 2206, 2279, 2366
MaeI	CTAG	1 cut(s)	780
MaeII	ACGT	3 cut(s)	366, 416, 842
MaeIII	GTNAC	8 cut(s)	237, 497, 838, 855, 1153, 1363, 2085, 2104
MalI	GATC	6 cut(s)	437, 807, 1382, 1538, 2178, 2202
MbiI	CCGCTC	2 cut(s)	96, 341
MboI	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
MfeI	CAATTG	1 cut(s)	1419
MflI	RGATCY	1 cut(s)	2176
MhlI	GDGCHC	2 cut(s)	445, 609
MlsI	TGGCCA	1 cut(s)	2049
MluNI	TGGCCA	1 cut(s)	2049
MlyI	GAGTC	6 cut(s)	146, 344, 1438, 1845, 1905, 2016
MmeI	TCCRAC	1 cut(s)	561
Mox20I	TGGCCA	1 cut(s)	2049
Mph1103I	ATGCAT	1 cut(s)	801
MroI	TCCGGA	1 cut(s)	712
MroXI	GAANNNNTTC	2 cut(s)	1244, 1601
MscI	TGGCCA	1 cut(s)	2049
MslI	CAYNNNNRTG	2 cut(s)	1452, 1610
Msp20I	TGGCCA	1 cut(s)	2049
MspCI	CTTAAG	1 cut(s)	2492
MspI	CCGG	2 cut(s)	399, 713
MspR9I	CCNGG	4 cut(s)	400, 479, 1493, 1769
MunI	CAATTG	1 cut(s)	1419
Mva1269I	GAATGC	3 cut(s)	63, 1743, 2226
MvaI	CCWGG	3 cut(s)	479, 1493, 1769
MvnI	CGCG	1 cut(s)	546
MwoI	GCNNNNNNNGC	2 cut(s)	552, 1740
NciI	CCSGG	1 cut(s)	400
NdeII	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
NlaIII	CATG	5 cut(s)	48, 1451, 1609, 1720, 2321
NlaIV	GGNNCC	1 cut(s)	50
NmeAIII	GCCGAG	1 cut(s)	697
NmuCI	GTSAC	4 cut(s)	237, 497, 1363, 2104
NsiI	ATGCAT	1 cut(s)	801
PacI	TTAATTAA	2 cut(s)	936, 1977
PaeR7I	CTCGAG	1 cut(s)	332
PcsI	WCGNNNNNNNCGW	1 cut(s)	216
PctI	GAATGC	3 cut(s)	63, 1743, 2226
PdmI	GAANNNNTTC	2 cut(s)	1244, 1601
PfeI	GAWTC	5 cut(s)	225, 766, 1637, 2264, 2524
PkrI	GCNGC	6 cut(s)	822, 825, 1278, 1326, 1459, 1932
PleI	GAGTC	6 cut(s)	146, 343, 1438, 1844, 1904, 2015
PpsI	GAGTC	6 cut(s)	146, 343, 1438, 1844, 1904, 2015
Ppu21I	YACGTR	1 cut(s)	367
PshBI	ATTAAT	2 cut(s)	936, 1973
PsiI	TTATAA	1 cut(s)	1755
Psp6I	CCWGG	3 cut(s)	477, 1491, 1767
PspEI	GGTNACC	1 cut(s)	1153
PspFI	CCCAGC	2 cut(s)	1574, 2511
PspGI	CCWGG	3 cut(s)	477, 1491, 1767
PspN4I	GGNNCC	1 cut(s)	50
PsrI	GAACNNNNNNTAC	2 cut(s)	2153, 2185
PsuI	RGATCY	1 cut(s)	2176
RsaI	GTAC	5 cut(s)	380, 524, 1165, 1855, 1874
RsaNI	GTAC	5 cut(s)	379, 523, 1164, 1854, 1873
RseI	CAYNNNNRTG	2 cut(s)	1452, 1610
SatI	GCNGC	6 cut(s)	821, 824, 1277, 1325, 1458, 1931
Sau3AI	GATC	6 cut(s)	435, 805, 1380, 1536, 2176, 2200
ScaI	AGTACT	1 cut(s)	1165
SchI	GAGTC	6 cut(s)	146, 344, 1438, 1845, 1905, 2016
ScrFI	CCNGG	4 cut(s)	400, 479, 1493, 1769
SduI	GDGCHC	2 cut(s)	445, 609
SfaNI	GCATC	8 cut(s)	126, 681, 1080, 1090, 1894, 2206, 2279, 2366
SfcI	CTRYAG	4 cut(s)	751, 1159, 2115, 2221
Sfr274I	CTCGAG	1 cut(s)	332
SlaI	CTCGAG	1 cut(s)	332
SmiMI	CAYNNNNRTG	2 cut(s)	1452, 1610
SmlI	CTYRAG	2 cut(s)	332, 2492
SmoI	CTYRAG	2 cut(s)	332, 2492
SsiI	CCGC	9 cut(s)	94, 115, 129, 199, 339, 546, 824, 1313, 1457
SspI	AATATT	5 cut(s)	786, 1138, 1794, 1954, 1971
SspMI	CTAG	1 cut(s)	780
StyD4I	CCNGG	4 cut(s)	398, 477, 1491, 1767
TaaI	ACNGT	4 cut(s)	1153, 1163, 1363, 1877
TaiI	ACGT	3 cut(s)	369, 419, 845
TaqI	TCGA	8 cut(s)	150, 333, 486, 638, 734, 1000, 1383, 2518
TatI	WGTACW	5 cut(s)	378, 522, 1163, 1853, 1872
TauI	GCSGC	2 cut(s)	826, 1460
TfiI	GAWTC	5 cut(s)	225, 766, 1637, 2264, 2524
TscAI	CASTG	3 cut(s)	577, 1647, 2476
TseFI	GTSAC	4 cut(s)	237, 497, 1363, 2104
TseI	GCWGC	4 cut(s)	820, 1276, 1324, 1930
Tsp45I	GTSAC	4 cut(s)	237, 497, 1363, 2104
TspDTI	ATGAA	9 cut(s)	1349, 1594, 1602, 1833, 1838, 1938, 2258, 2343, 2568
TspGWI	ACGGA	3 cut(s)	818, 1085, 1191
TspRI	CASTG	3 cut(s)	577, 1647, 2476
Vha464I	CTTAAG	1 cut(s)	2492
VspI	ATTAAT	2 cut(s)	936, 1973
XapI	RAATTY	2 cut(s)	1477, 2555
XbaI	TCTAGA	1 cut(s)	779
XhoI	CTCGAG	1 cut(s)	332
XmiI	GTMKAC	1 cut(s)	419
XmnI	GAANNNNTTC	2 cut(s)	1244, 1601
XspI	CTAG	1 cut(s)	780
ZrmI	AGTACT	1 cut(s)	1165
Zsp2I	ATGCAT	1 cut(s)	801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.