Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004810

Physical Location & Seq

Genomic Coordinates Forward (+)

25193961 .. 25198113

Gene Structure 4153 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013030

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGAGCAAGCAACCTCACTCACCAAAGCTCTACTCAACAACACCAACAATCCCAAACTCGCATGGCACCTCTTCAAGCGCATTCTCTCCTCCCCCACTTCCTCCTCCTCACATCTCTCCCTCCGCTCCCTCACCATCATAGCCCGCATCCTCATCTCCGCCAATATGCACCCCCAAATCGACGCCCTCCACCACCTCCTCCTCCGCTCACAGCCCCCCCAAACCCTCCGCCCTTGCCTCGTTTCCCTCGTGCGAATCTTCGCCAAGTCACGCCTCGCCGACGAGACCCTTTCGCATTTTAAGTCTCTCCGAAGCCACTTCCCCAATGACCCTCCTTCTGTTTATTTGTATAACTTGCTTCTCGAGTCCGCTCTCAGGGAGAACGATGTGGATTGCGTTTTGTGGTTGTATAAGGATATGATTGTTTCCGGGGTTAAGCCCGAAACGTATACTTTTAATCTTCTGATCTGTGCATTGTGTGAATGTGGTCGTTTGGAAGATGCACGCCAGGTGTTCGATAAAATGCGTGAGAAGGGTTGTCAGCCGAATGAGTACACCGTTGGGATTTTGGTTCGTGGGTATTGCAGAGCTGGGTTGAGCAGTGGAGGATTGGAGGTTTTGGATTGTGGTGCTGTGCCTAATAGGGTTGTGTATAATACTTTGGTTTCGAGTTTTTGTAAACAAGGCAGGACTGATGAGGCCGAGAAATTGGTGGAGAGGATGCGAGAGGAGGGTATGTTTCCGGATGTCATTACTTTCAATTCAAGGATATCGGCTCTATGTAGTGCAGGGAAGATTCTTGAAGCGTCCAGAATATTTAGAGATATGCATATGGATCGGGCTTTGGGCCTGCCGCAACCCAATGTTGTAACGTATAATCTGATGTTACAAGGATTTTGCAAGGAAGGGATGTTAGAGGAAGCAGAGAGCTTGTTCAAATCTATGGAAAAAGCTGGTGGTTTAATTAATATAGAGACCTATAATATATGGTTGTTGGGTTTGGTCAGGAATAAGAAGCTCTTGGAGGCTCGACTGGTTCTGAAAGAGATGGTAGATAAGGGAATAGAACCCAACATTTACTCATATAACATTGTGATAAATGGGTTATGCAAAAATGGGATGCTCCGTGATGCAAGAATTGTGATGGGTCTGATGGCAAGAAACAATATTTCGCCAGATACGGTTACCTACAGTACTTTACTACATGGATACTGCAATAAAGGGAAGGTATTTGAAGCCAACAATGTTCTGCAAGAGATGATAACAAAGAATTGCTTCCCAAATACTCATACTTGCAACATTCTGCTGCACAGTTTATGGAAAGAGGGAAGAACATCAGAAGCGGAGGAACTGCTGCAAAAGATGAATGAGAGAGGTTATGGCTTAGATACTGTGACCTGCAACATCGTGATCGATGGTCTGTGCAATGGTGGGAAATTGGACAAAGCAATTGAAATTGTAAGTGGGATGTGGACTCATGGAAGTGCGGCTCTTGGAAACTTAGGGAATTCCTTTGTTGGCCTGGTTGATGATAGTAATAATGGGAATAAATGCCTTCCAGATTTGATCTCATATTCAACCATAATTAGTGGGTTATGCAAGGCTGGGAGGCTTGATGAAGCTAAGAAGAAGTTCATGGAGATGATGGGCAAAAACTTGCACCCTGATTCAGTGATTTATGATACTTTTATACATAGCTTTTGTAAACAAGGAAGGATATCATCGGCATTTCGGGTGTTGAAGGACATGGAGAAGAAAGGTTGCAACAAGAGCATTCAAACTTATAATTCACTAATCCTGGGCTTAGGGAGTAAAAAGCAAATATTTGAAATATATGGGCTGATGGATGAAATGAAAGAGAGGGGAGTCCCTCCTGATGTCTGTACTTACAATAATATGATGTACTGTCTCTGTGAAGTAGAGAGAGTCAAAGATGCAACTTCTCTTTTAGATGAAATGCTGCAAAAGGGCATATCCCCGAATATTTCTACCTTCAGAATATTAATTAAAGCTTTCTGCAAGGGTTGTGATTTTGGAGTCGCACAGGAGTTATTTGACATTGCTTTAAGTGTATGTGGCCACAAGGAAGCCTTATATAGCTTGATGTTTAATGAGTTACTTGCTGGAGGGGAAGTCGCAAAAGCTACAGAGCTATTTGAAGAAGCATTGGACAAGTATTTTTATCTGGGGAACTTCCTTTACAAAGATCTCATTGATAGACTTTGCAAGGATCAAAAGTTAGAGGATGCCTGTAGCATTCTTCACAATATGATGAATAAGGGATACGGGTTTGATTCAGCATCCTTCCTACCAGTAATTGATGGCTTGGGTAAAAGGGGGAATAAGCATGAGGCTGATGAACTTGCAGAGAGAATGATGGAAATGGCATCAGAAGGTAGGATAGCAAATAAGGTTTACCGAAATGAGAGGGATATAATTGGTGGAAAACCAAAGTCTCATGGAAGTGATTGGCAGACCATAGTGAACAGAGATGATGGCAGTGGAATCTTATTGAAAACTCTTAAGAGGGCCAAGAGACGACTAACCTTAGTATCTAAATTCTCAGCTATGTTGAAGGAAGCTAGAATCCAGTTACCTCCAGAGTCCAGATTGATTTGTCATTCACGGACATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

879

Amino Acids

98.58

Weight (kDa)

8.33

Isoelectric Point (pI)

38.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	112 - 160	9.9e-09	PPR repeat family
PPR_3	PF13812	137 - 185	5.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	144 - 176	1.1e-10	PPR repeat
PPR_2	PF13041	147 - 196	1.5e-16	PPR repeat family
PPR	PF01535	150 - 180	4.1e-08	PPR repeat
PPR_1	PF12854	210 - 242	3e-12	PPR repeat
PPR_3	PF13812	213 - 259	1.3e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	213 - 261	8.8e-14	PPR repeat family
PPR	PF01535	216 - 245	1.8e-08	PPR repeat
PPR_1	PF12854	245 - 276	3.5e-08	PPR repeat
PPR_1	PF12854	286 - 316	2.4e-11	PPR repeat
PPR_2	PF13041	287 - 335	4.2e-14	PPR repeat family
PPR	PF01535	290 - 319	7.3e-10	PPR repeat
PPR_long	PF17177	300 - 457	1.7e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	310 - 367	3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	323 - 371	8.3e-14	PPR repeat family
PPR_1	PF12854	353 - 378	2.9e-09	PPR repeat
PPR_2	PF13041	360 - 406	1.8e-14	PPR repeat family
TPR_24	PF23276	378 - 486	9.4e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	385 - 437	3e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	389 - 420	3.3e-11	PPR repeat
PPR_2	PF13041	392 - 439	1.4e-14	PPR repeat family
PPR	PF01535	395 - 425	6.8e-07	PPR repeat
PPR_2	PF13041	428 - 476	4.1e-11	PPR repeat family
PPR_1	PF12854	459 - 490	3.4e-06	PPR repeat
PPR_1	PF12854	518 - 548	7.5e-12	PPR repeat
PPR_2	PF13041	520 - 558	6.5e-08	PPR repeat family
PPR	PF01535	523 - 552	9.4e-07	PPR repeat
PPR_3	PF13812	546 - 599	1.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	551 - 584	3.8e-10	PPR repeat
PPR_2	PF13041	555 - 602	2.7e-13	PPR repeat family
PPR_long	PF17177	572 - 673	2.5e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	591 - 638	5.1e-12	PPR repeat family
PPR_3	PF13812	614 - 672	6.1e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	621 - 653	5e-08	PPR repeat
PPR_2	PF13041	625 - 674	1.7e-16	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004810)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17140 AT2G17140
fragaria_vesca	FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640 FvH4_2g16640
malus_domestica	MD05G1134600.v1.1 MD05G1134700.v1.1 MD10G1139700.v1.1
prunus_persica	Prupe.8G178500_v2.0.a1 Prupe.8G178500_v2.0.a1
pyrus_communis	pycom05g13140 pycom10g11860
rosa_chinensis	RchiOBHm_Chr6g0280761
rosa_laevigata	RLG00000013030
rosa_multiflora	Rmu_co8435227.1_g000001 Rmu_sc0042093.1_g000001 Rmu_sc0042110.1_g000001
rosa_roxburghii	Rroxscaffold_1G00044300 Rroxscaffold_6G00391320 Rroxscaffold_6G00391330 Rroxscaffold_7G00187700
rosa_rugosa	Rorug06G0134300
rosa_samantha	Rh6AG247000 Rh6BG249100 Rh6CG250400 Rh6DG241800
rosa_wichuraiana	Rw6G021390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1785
Acc36I	ACCTGC	1 cut(s)	1406
AccB1I	GGYRCC	1 cut(s)	66
AccBSI	CCGCTC	3 cut(s)	126, 207, 371
AccI	GTMKAC	1 cut(s)	449
AccIII	TCCGGA	1 cut(s)	742
AciI	CCGC	9 cut(s)	124, 145, 159, 205, 229, 369, 854, 1343, 1487
AclWI	GGATC	2 cut(s)	843, 2238
AcoI	YGGCCR	1 cut(s)	2077
AcsI	RAATTY	2 cut(s)	1507, 2558
AcuI	CTGAAG	1 cut(s)	1978
AcyI	GRCGYC	1 cut(s)	183
AfaI	GTAC	4 cut(s)	554, 1195, 1885, 1904
AfiI	CCNNNNNNNGG	1 cut(s)	375
AflII	CTTAAG	1 cut(s)	2522
AjnI	CCWGG	3 cut(s)	507, 1521, 1797
Alw26I	GTCTC	6 cut(s)	278, 309, 968, 1913, 2460, 2530
AlwI	GGATC	2 cut(s)	843, 2238
Ama87I	CYCGRG	1 cut(s)	362
Aor13HI	TCCGGA	1 cut(s)	742
AoxI	GGCC	5 cut(s)	699, 847, 1519, 2077, 2529
ApeKI	GCWGC	3 cut(s)	1306, 1354, 1960
ApoI	RAATTY	2 cut(s)	1507, 2558
AseI	ATTAAT	2 cut(s)	966, 2003
Asp700I	GAANNNNTTC	2 cut(s)	1274, 1631
AspLEI	GCGC	1 cut(s)	81
AspS9I	GGNCC	2 cut(s)	847, 2529
AsuC2I	CCSGG	1 cut(s)	430
AsuHPI	GGTGA	2 cut(s)	13, 124
AvaI	CYCGRG	1 cut(s)	362
BaeI	ACNNNNGTAYC	2 cut(s)	2535, 2568
BalI	TGGCCA	1 cut(s)	2079
BanI	GGYRCC	1 cut(s)	66
BauI	CACGAG	1 cut(s)	248
BbvI	GCAGC	3 cut(s)	1293, 1341, 1947
BcgI	CGANNNNNNTGC	2 cut(s)	818, 852
BciT130I	CCWGG	3 cut(s)	509, 1523, 1799
BciVI	GTATCC	2 cut(s)	1202, 2276
BcnI	CCSGG	1 cut(s)	430
BcoDI	GTCTC	6 cut(s)	278, 309, 968, 1913, 2460, 2530
BfaI	CTAG	1 cut(s)	2583
BfmI	CTRYAG	3 cut(s)	1189, 2145, 2251
BfrI	CTTAAG	1 cut(s)	2522
BfuAI	ACCTGC	1 cut(s)	1406
BfuI	GTATCC	2 cut(s)	1202, 2276
BglII	AGATCT	1 cut(s)	2206
BisI	GCNGC	5 cut(s)	854, 1307, 1355, 1488, 1961
BlsI	GCNGC	5 cut(s)	855, 1308, 1356, 1489, 1962
BmcAI	AGTACT	1 cut(s)	1195
Bme1390I	CCNGG	4 cut(s)	430, 509, 1523, 1799
BmeT110I	CYCGRG	1 cut(s)	362
BmgT120I	GGNCC	2 cut(s)	847, 2529
BmiI	GGNNCC	1 cut(s)	68
BmrFI	CCNGG	4 cut(s)	430, 509, 1523, 1799
BmsI	GCATC	9 cut(s)	156, 490, 711, 1110, 1120, 1924, 2236, 2309, 2396
BpmI	CTGGAG	2 cut(s)	2145, 2583
Bpu10I	CCTNAGC	1 cut(s)	1804
BpuMI	CCSGG	1 cut(s)	430
Bsa29I	ATCGAT	1 cut(s)	1413
BsaHI	GRCGYC	1 cut(s)	183
BsaI	GGTCTC	2 cut(s)	278, 968
BsaJI	CCNNGG	2 cut(s)	429, 1798
BsaWI	WCCGGW	1 cut(s)	742
BsaXI	ACNNNNNCTCC	4 cut(s)	722, 752, 2118, 2148
Bsc4I	CCNNNNNNNGG	1 cut(s)	375
Bse1I	ACTGG	3 cut(s)	1038, 2312, 2590
Bse3DI	GCAATG	2 cut(s)	1432, 2058
BseAI	TCCGGA	1 cut(s)	742
BseBI	CCWGG	3 cut(s)	509, 1523, 1799
BseCI	ATCGAT	1 cut(s)	1413
BseDI	CCNNGG	2 cut(s)	429, 1798
BseGI	GGATG	9 cut(s)	147, 726, 751, 915, 1125, 1473, 1852, 2251, 2300
BseLI	CCNNNNNNNGG	1 cut(s)	375
BseMI	GCAATG	2 cut(s)	1432, 2058
BseMII	CTCAG	2 cut(s)	388, 2577
BseNI	ACTGG	3 cut(s)	1038, 2312, 2590
BseRI	GAGGAG	6 cut(s)	79, 94, 97, 188, 191, 743
BseXI	GCAGC	3 cut(s)	1293, 1341, 1947
BseYI	CCCAGC	2 cut(s)	590, 1604
BsgI	GTGCAG	2 cut(s)	807, 1292
BshFI	GGCC	5 cut(s)	701, 849, 1521, 2079, 2531
BshNI	GGYRCC	1 cut(s)	66
BshVI	ATCGAT	1 cut(s)	1413
BsiHKCI	CYCGRG	1 cut(s)	362
BsiSI	CCGG	2 cut(s)	429, 743
BslFI	GGGAC	1 cut(s)	1853
BslI	CCNNNNNNNGG	1 cut(s)	375
BsmAI	GTCTC	6 cut(s)	278, 309, 968, 1913, 2460, 2530
BsmBI	CGTCTC	1 cut(s)	2530
BsmFI	GGGAC	1 cut(s)	1853
BsmI	GAATGC	3 cut(s)	81, 1773, 2256
BsnI	GGCC	5 cut(s)	701, 849, 1521, 2079, 2531
Bso31I	GGTCTC	2 cut(s)	278, 968
BsoBI	CYCGRG	1 cut(s)	362
Bsp13I	TCCGGA	1 cut(s)	742
Bsp143I	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
BspACI	CCGC	9 cut(s)	124, 145, 159, 205, 229, 369, 854, 1343, 1487
BspANI	GGCC	5 cut(s)	701, 849, 1521, 2079, 2531
BspCNI	CTCAG	2 cut(s)	387, 2576
BspDI	ATCGAT	1 cut(s)	1413
BspEI	TCCGGA	1 cut(s)	742
BspLI	GGNNCC	1 cut(s)	68
BspMI	ACCTGC	1 cut(s)	1406
BspPI	GGATC	2 cut(s)	843, 2238
BspT107I	GGYRCC	1 cut(s)	66
BspTI	CTTAAG	1 cut(s)	2522
BspTNI	GGTCTC	2 cut(s)	278, 968
BsrBI	CCGCTC	3 cut(s)	126, 207, 371
BsrDI	GCAATG	2 cut(s)	1432, 2058
BsrI	ACTGG	3 cut(s)	1038, 2312, 2590
BssECI	CCNNGG	2 cut(s)	429, 1798
BssMI	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
BssNAI	GTATAC	1 cut(s)	450
BssNI	GRCGYC	1 cut(s)	183
BssSI	CACGAG	1 cut(s)	248
Bst1107I	GTATAC	1 cut(s)	450
Bst2BI	CACGAG	1 cut(s)	248
Bst2UI	CCWGG	3 cut(s)	509, 1523, 1799
Bst4CI	ACNGT	6 cut(s)	559, 1183, 1193, 1313, 1393, 1907
Bst6I	CTCTTC	1 cut(s)	77
BstACI	GRCGYC	1 cut(s)	183
BstAFI	CTTAAG	1 cut(s)	2522
BstC8I	GCNNGC	4 cut(s)	9, 145, 505, 851
BstDEI	CTNAG	7 cut(s)	374, 1384, 1501, 1623, 1804, 2548, 2563
BstEII	GGTNACC	1 cut(s)	1183
BstF5I	GGATG	9 cut(s)	147, 726, 751, 915, 1125, 1473, 1852, 2251, 2300
BstHHI	GCGC	1 cut(s)	81
BstKTI	GATC	6 cut(s)	468, 838, 1413, 1569, 2209, 2233
BstMAI	GTCTC	6 cut(s)	278, 309, 968, 1913, 2460, 2530
BstMBI	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
BstMWI	GCNNNNNNNGC	1 cut(s)	1770
BstNI	CCWGG	3 cut(s)	509, 1523, 1799
BstPI	GGTNACC	1 cut(s)	1183
BstSCI	CCNGG	4 cut(s)	428, 507, 1521, 1797
BstSFI	CTRYAG	3 cut(s)	1189, 2145, 2251
BstV1I	GCAGC	3 cut(s)	1293, 1341, 1947
BstX2I	RGATCY	1 cut(s)	2206
BstYI	RGATCY	1 cut(s)	2206
BstZ17I	GTATAC	1 cut(s)	450
Bsu15I	ATCGAT	1 cut(s)	1413
BsuI	GTATCC	2 cut(s)	1202, 2276
BsuRI	GGCC	5 cut(s)	701, 849, 1521, 2079, 2531
BsuTUI	ATCGAT	1 cut(s)	1413
BtsCI	GGATG	9 cut(s)	147, 726, 751, 915, 1125, 1473, 1852, 2251, 2300
BtsI	GCAGTG	2 cut(s)	607, 2506
BtsIMutI	CAGTG	3 cut(s)	607, 1677, 2506
BveI	ACCTGC	1 cut(s)	1406
Cac8I	GCNNGC	4 cut(s)	9, 145, 505, 851
CfoI	GCGC	1 cut(s)	81
Cfr13I	GGNCC	2 cut(s)	847, 2529
ClaI	ATCGAT	1 cut(s)	1413
CseI	GACGC	2 cut(s)	191, 795
Csp6I	GTAC	4 cut(s)	553, 1194, 1884, 1903
CviAII	CATG	8 cut(s)	63, 1205, 1478, 1636, 1747, 2348, 2459, 2631
CviQI	GTAC	4 cut(s)	553, 1194, 1884, 1903
DdeI	CTNAG	7 cut(s)	374, 1384, 1501, 1623, 1804, 2548, 2563
DpnI	GATC	6 cut(s)	467, 837, 1412, 1568, 2208, 2232
DpnII	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
EaeI	YGGCCR	1 cut(s)	2077
Eam1104I	CTCTTC	1 cut(s)	77
EarI	CTCTTC	1 cut(s)	77
EciI	GGCGGA	2 cut(s)	148, 218
Eco31I	GGTCTC	2 cut(s)	278, 968
Eco32I	GATATC	2 cut(s)	771, 1719
Eco57I	CTGAAG	1 cut(s)	1978
Eco88I	CYCGRG	1 cut(s)	362
Eco91I	GGTNACC	1 cut(s)	1183
EcoO65I	GGTNACC	1 cut(s)	1183
EcoRI	GAATTC	1 cut(s)	1507
EcoRII	CCWGG	3 cut(s)	507, 1521, 1797
EcoRV	GATATC	2 cut(s)	771, 1719
EcoT22I	ATGCAT	1 cut(s)	831
Esp3I	CGTCTC	1 cut(s)	2530
FaeI	CATG	8 cut(s)	66, 1208, 1481, 1639, 1750, 2351, 2462, 2634
FalI	AAGNNNNNCTT	6 cut(s)	1004, 1036, 1259, 1291, 2075, 2107
FaqI	GGGAC	1 cut(s)	1853
FatI	CATG	8 cut(s)	62, 1204, 1477, 1635, 1746, 2347, 2458, 2630
FauI	CCCGC	1 cut(s)	152
FauNDI	CATATG	1 cut(s)	831
FblI	GTMKAC	1 cut(s)	449
Fnu4HI	GCNGC	5 cut(s)	854, 1307, 1355, 1488, 1961
FokI	GGATG	9 cut(s)	134, 733, 758, 922, 1132, 1480, 1859, 2258, 2287
Fsp4HI	GCNGC	5 cut(s)	854, 1307, 1355, 1488, 1961
FspBI	CTAG	1 cut(s)	2583
GlaI	GCGC	1 cut(s)	80
GluI	GCNGC	5 cut(s)	854, 1307, 1355, 1488, 1961
GsaI	CCCAGC	2 cut(s)	594, 1608
GsuI	CTGGAG	2 cut(s)	2145, 2583
HaeIII	GGCC	5 cut(s)	701, 849, 1521, 2079, 2531
HapII	CCGG	2 cut(s)	429, 743
HgaI	GACGC	2 cut(s)	191, 795
HhaI	GCGC	1 cut(s)	81
Hin1I	GRCGYC	1 cut(s)	183
Hin1II	CATG	8 cut(s)	66, 1208, 1481, 1639, 1750, 2351, 2462, 2634
Hin6I	GCGC	1 cut(s)	79
HinP1I	GCGC	1 cut(s)	79
HindIII	AAGCTT	1 cut(s)	2010
HpaII	CCGG	2 cut(s)	429, 743
HphI	GGTGA	2 cut(s)	13, 124
Hpy166II	GTNNAC	7 cut(s)	450, 555, 680, 1473, 1706, 2416, 2485
Hpy188I	TCNGA	8 cut(s)	311, 465, 882, 1041, 1152, 1339, 1997, 2392
Hpy8I	GTNNAC	7 cut(s)	450, 555, 680, 1473, 1706, 2416, 2485
Hpy99I	CGWCG	2 cut(s)	185, 284
HpyCH4III	ACNGT	6 cut(s)	559, 1183, 1193, 1313, 1393, 1907
HpyCH4IV	ACGT	2 cut(s)	446, 872
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1770
HpyF3I	CTNAG	7 cut(s)	374, 1384, 1501, 1623, 1804, 2548, 2563
HpySE526I	ACGT	2 cut(s)	446, 872
Hsp92I	GRCGYC	1 cut(s)	183
Hsp92II	CATG	8 cut(s)	66, 1208, 1481, 1639, 1750, 2351, 2462, 2634
HspAI	GCGC	1 cut(s)	79
Kpn2I	TCCGGA	1 cut(s)	742
Kzo9I	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
LmnI	GCTCC	3 cut(s)	4, 131, 1128
Lsp1109I	GCAGC	3 cut(s)	1293, 1341, 1947
LweI	GCATC	9 cut(s)	156, 490, 711, 1110, 1120, 1924, 2236, 2309, 2396
MaeI	CTAG	1 cut(s)	2583
MaeII	ACGT	2 cut(s)	446, 872
MaeIII	GTNAC	7 cut(s)	267, 868, 885, 1183, 1393, 2115, 2592
MalI	GATC	6 cut(s)	467, 837, 1412, 1568, 2208, 2232
MbiI	CCGCTC	3 cut(s)	126, 207, 371
MboI	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
MfeI	CAATTG	1 cut(s)	1449
MflI	RGATCY	1 cut(s)	2206
MlsI	TGGCCA	1 cut(s)	2079
MluNI	TGGCCA	1 cut(s)	2079
MlyI	GAGTC	6 cut(s)	374, 1468, 1875, 1935, 2046, 2612
Mox20I	TGGCCA	1 cut(s)	2079
Mph1103I	ATGCAT	1 cut(s)	831
MroI	TCCGGA	1 cut(s)	742
MroXI	GAANNNNTTC	2 cut(s)	1274, 1631
MscI	TGGCCA	1 cut(s)	2079
MslI	CAYNNNNRTG	4 cut(s)	1482, 1640, 2463, 2629
Msp20I	TGGCCA	1 cut(s)	2079
MspCI	CTTAAG	1 cut(s)	2522
MspI	CCGG	2 cut(s)	429, 743
MspR9I	CCNGG	4 cut(s)	430, 509, 1523, 1799
MunI	CAATTG	1 cut(s)	1449
Mva1269I	GAATGC	3 cut(s)	81, 1773, 2256
MvaI	CCWGG	3 cut(s)	509, 1523, 1799
MwoI	GCNNNNNNNGC	1 cut(s)	1770
NciI	CCSGG	1 cut(s)	430
NdeI	CATATG	1 cut(s)	831
NdeII	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
NlaIII	CATG	8 cut(s)	66, 1208, 1481, 1639, 1750, 2351, 2462, 2634
NlaIV	GGNNCC	1 cut(s)	68
NmeAIII	GCCGAG	1 cut(s)	727
NmuCI	GTSAC	2 cut(s)	267, 1393
NsiI	ATGCAT	1 cut(s)	831
PacI	TTAATTAA	2 cut(s)	966, 2007
PaeR7I	CTCGAG	1 cut(s)	362
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PctI	GAATGC	3 cut(s)	81, 1773, 2256
PdmI	GAANNNNTTC	2 cut(s)	1274, 1631
PfeI	GAWTC	6 cut(s)	255, 796, 1667, 2294, 2505, 2586
PkrI	GCNGC	5 cut(s)	855, 1308, 1356, 1489, 1962
PleI	GAGTC	6 cut(s)	373, 1468, 1874, 1934, 2045, 2611
PpsI	GAGTC	6 cut(s)	373, 1468, 1874, 1934, 2045, 2611
PshBI	ATTAAT	2 cut(s)	966, 2003
PsiI	TTATAA	1 cut(s)	1785
Psp6I	CCWGG	3 cut(s)	507, 1521, 1797
PspEI	GGTNACC	1 cut(s)	1183
PspFI	CCCAGC	2 cut(s)	590, 1604
PspGI	CCWGG	3 cut(s)	507, 1521, 1797
PspN4I	GGNNCC	1 cut(s)	68
PspPI	GGNCC	2 cut(s)	847, 2529
PsrI	GAACNNNNNNTAC	2 cut(s)	2183, 2215
PsuI	RGATCY	1 cut(s)	2206
RsaI	GTAC	4 cut(s)	554, 1195, 1885, 1904
RsaNI	GTAC	4 cut(s)	553, 1194, 1884, 1903
RseI	CAYNNNNRTG	4 cut(s)	1482, 1640, 2463, 2629
SatI	GCNGC	5 cut(s)	854, 1307, 1355, 1488, 1961
Sau3AI	GATC	6 cut(s)	465, 835, 1410, 1566, 2206, 2230
Sau96I	GGNCC	2 cut(s)	847, 2529
ScaI	AGTACT	1 cut(s)	1195
SchI	GAGTC	6 cut(s)	374, 1468, 1875, 1935, 2046, 2612
ScrFI	CCNGG	4 cut(s)	430, 509, 1523, 1799
SfaNI	GCATC	9 cut(s)	156, 490, 711, 1110, 1120, 1924, 2236, 2309, 2396
SfcI	CTRYAG	3 cut(s)	1189, 2145, 2251
Sfr274I	CTCGAG	1 cut(s)	362
SlaI	CTCGAG	1 cut(s)	362
SmiMI	CAYNNNNRTG	4 cut(s)	1482, 1640, 2463, 2629
SmlI	CTYRAG	2 cut(s)	362, 2522
SmoI	CTYRAG	2 cut(s)	362, 2522
SsiI	CCGC	9 cut(s)	124, 145, 159, 205, 229, 369, 854, 1343, 1487
SspI	AATATT	5 cut(s)	816, 1168, 1824, 1984, 2001
SspMI	CTAG	1 cut(s)	2583
StyD4I	CCNGG	4 cut(s)	428, 507, 1521, 1797
TaaI	ACNGT	6 cut(s)	559, 1183, 1193, 1313, 1393, 1907
TaiI	ACGT	2 cut(s)	449, 875
TaqI	TCGA	6 cut(s)	180, 363, 516, 668, 1030, 1413
TatI	WGTACW	4 cut(s)	552, 1193, 1883, 1902
TauI	GCSGC	2 cut(s)	856, 1490
TfiI	GAWTC	6 cut(s)	255, 796, 1667, 2294, 2505, 2586
TscAI	CASTG	3 cut(s)	607, 1677, 2506
TseFI	GTSAC	2 cut(s)	267, 1393
TseI	GCWGC	3 cut(s)	1306, 1354, 1960
Tsp45I	GTSAC	2 cut(s)	267, 1393
TspDTI	ATGAA	8 cut(s)	1379, 1624, 1632, 1863, 1868, 1968, 2288, 2373
TspGWI	ACGGA	1 cut(s)	1115
TspRI	CASTG	3 cut(s)	607, 1677, 2506
Vha464I	CTTAAG	1 cut(s)	2522
VspI	ATTAAT	2 cut(s)	966, 2003
XapI	RAATTY	2 cut(s)	1507, 2558
XhoI	CTCGAG	1 cut(s)	362
XmiI	GTMKAC	1 cut(s)	449
XmnI	GAANNNNTTC	2 cut(s)	1274, 1631
XspI	CTAG	1 cut(s)	2583
ZrmI	AGTACT	1 cut(s)	1195
Zsp2I	ATGCAT	1 cut(s)	831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.