MD05G1145600.v1.1

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004589
Physical Location & Seq
Forward (+)
27669751 .. 27671653
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1145600.v1.1.491

Sequence Viewer

Length: 486 bp
ATGATTCAATTTGTGCTTCTAATAAGCAGACAAGGAAAAGTGAGGTTGACAAAATGGTATTCTCCTTATACCCAAAAGGAAAGAAGTAAGGTTCTCCGTGAGCTCAGTGGAGTAATTCTTGCGCGAGGTCCCAAACTCTGTAATTTTGTGGATTGGAGAGGATACAAAGTTGTTTATAAAAGATATGCCAGTCTGTATTTCTGCATGTGTATTGATCAAGAAGATAACGAATTAGAGGTCCTTGAAATGATTCATCATTATGTTGAGATTCTCGACCGATACTTTGGCAGTGTCTGTGAATTGGATTTGATATTTAACTTCCACAAGGCCTACTATATACTAGATGAAATTTTGATTGCTGGTGAACTTCAAGAATCGAGCAAGAAAACAGTTGCACGATTGATAGCTGCACAGGATTCTTTGGTGGAGACTGCAAAAGAGCAGGCTAGTTCGATAAGTAATATAATCGCGCAGGCAACCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

162

Amino Acids

18.71

Weight (kDa)

7.64

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 1 - 140 7.4e-55 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccII CGCG 2 cut(s) 124, 470
AcsI RAATTY 1 cut(s) 348
AgsI TTSAA 3 cut(s) 8, 245, 371
AluBI AGCT 2 cut(s) 103, 407
AluI AGCT 2 cut(s) 103, 407
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 1 cut(s) 348
ArsI GACNNNNNNTTYG 4 cut(s) 222, 254, 266, 298
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 2 cut(s) 124, 472
AspS9I GGNCC 2 cut(s) 128, 238
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 128, 238
BanII GRGCYC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 394
BciVI GTATCC 1 cut(s) 155
BclI TGATCA 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 341, 447
BfuI GTATCC 1 cut(s) 155
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme18I GGWCC 2 cut(s) 128, 238
BmgT120I GGNCC 2 cut(s) 128, 238
BmiI GGNNCC 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 2 cut(s) 124, 470
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 105
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 1 cut(s) 214
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 1 cut(s) 117
BspFNI CGCG 2 cut(s) 124, 470
BspLI GGNNCC 1 cut(s) 130
BsrI ACTGG 1 cut(s) 189
BssMI GATC 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 444, 474
BstDEI CTNAG 1 cut(s) 104
BstFNI CGCG 2 cut(s) 124, 470
BstHHI GCGC 2 cut(s) 124, 472
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 1 cut(s) 214
BstMCI CGRYCG 1 cut(s) 277
BstNSI RCATGY 1 cut(s) 208
BstUI CGCG 2 cut(s) 124, 470
BstV1I GCAGC 1 cut(s) 394
BsuI GTATCC 1 cut(s) 155
BsuRI GGCC 1 cut(s) 329
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 112, 295
Cac8I GCNNGC 2 cut(s) 444, 474
CaiI CAGNNNCTG 1 cut(s) 294
CfoI GCGC 2 cut(s) 124, 472
Cfr13I GGNCC 2 cut(s) 128, 238
CviAII CATG 1 cut(s) 205
CviJI RGCY 4 cut(s) 103, 329, 407, 446
CviKI_1 RGCY 4 cut(s) 103, 329, 407, 446
DdeI CTNAG 1 cut(s) 104
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 128, 238
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 2 cut(s) 128, 238
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 1 cut(s) 208
FaiI YATR 8 cut(s) 69, 177, 186, 206, 261, 336, 338, 464
FaqI GGGAC 1 cut(s) 114
FatI CATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 408
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 2 cut(s) 341, 447
GlaI GCGC 2 cut(s) 123, 471
GluI GCNGC 1 cut(s) 408
HaeIII GGCC 1 cut(s) 329
HhaI GCGC 2 cut(s) 124, 472
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 2 cut(s) 122, 470
HinP1I GCGC 2 cut(s) 122, 470
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 5 cut(s) 4, 250, 268, 374, 416
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 48, 365
Hpy188III TCNNGA 3 cut(s) 218, 272, 371
Hpy8I GTNNAC 2 cut(s) 48, 365
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 4 cut(s) 204, 395, 410, 434
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 2 cut(s) 122, 470
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 1 cut(s) 214
LpnPI CCDG 5 cut(s) 202, 345, 398, 428, 458
Lsp1109I GCAGC 1 cut(s) 394
MaeI CTAG 2 cut(s) 341, 447
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 1 cut(s) 233
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 6 cut(s) 8, 114, 142, 230, 299, 348
MnlI CCTC 4 cut(s) 36, 119, 152, 229
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 258
MvnI CGCG 2 cut(s) 124, 470
NdeII GATC 1 cut(s) 214
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 1 cut(s) 130
NspI RCATGY 1 cut(s) 208
PceI AGGCCT 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 5 cut(s) 4, 250, 268, 374, 416
PkrI GCNGC 1 cut(s) 409
PpuMI RGGWCCY 2 cut(s) 128, 238
PsiI TTATAA 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 2 cut(s) 128, 238
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 2 cut(s) 128, 238
PspPPI RGGWCCY 2 cut(s) 128, 238
PstNI CAGNNNCTG 1 cut(s) 294
RseI CAYNNNNRTG 1 cut(s) 258
SacI GAGCTC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 2 cut(s) 128, 238
SduI GDGCHC 1 cut(s) 105
SetI ASST 6 cut(s) 47, 93, 105, 130, 240, 409
SinI GGWCC 2 cut(s) 128, 238
SmiMI CAYNNNNRTG 1 cut(s) 258
Sse9I AATT 6 cut(s) 8, 114, 142, 230, 299, 348
SseBI AGGCCT 1 cut(s) 329
SspMI CTAG 2 cut(s) 341, 447
SstI GAGCTC 1 cut(s) 105
StuI AGGCCT 1 cut(s) 329
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 273, 377, 452
TaqII GACCGA 1 cut(s) 291
TasI AATT 6 cut(s) 8, 114, 142, 230, 299, 348
TfiI GAWTC 5 cut(s) 4, 250, 268, 374, 416
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 2 cut(s) 112, 295
TseI GCWGC 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 242, 360
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 112, 295
VpaK11BI GGWCC 2 cut(s) 128, 238
XapI RAATTY 1 cut(s) 348
XceI RCATGY 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 2 cut(s) 341, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.