MD10G1145000.v1.1

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004589
Physical Location & Seq
Forward (+)
23137155 .. 23139150
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1145000.v1.1.491

Sequence Viewer

Length: 486 bp
ATGATTCATTTTGTGCTTCTTATAAGCAGACAAGGAAAAGTGAGGTTGACAAAATGGTATTCTCCTTACACTCAAAAGGAAAGAAATAAGGTTCTTCGTGAGCTTAGTGGAGTGATTCTTGCACGAGGTCCCAAGCTCTGTAATTTTGTGGATTGGAGAGGATACAAAGTTGTTTATAAAAGATATGCTAGTCTGTATTTCTGCATGTGTATTGATCAAGAAGATAACGAATTAGAGGTCCTTGAAATGATTCATCATTTTGTTGAGATTCTCGACCGATACTTTGGCAGCGTCTGTGAATTGGATTTGATCTTTAACTTCCACAAGGCCTACTATATACTGGATGAAATTTTGATTGCTGGTGAACTTCAAGAATCGAGCAAGAAAACAGTTGCGCGGTTGATAGCTGCGCAGGATTCATTGGTGGAGACTGCAAAAGAGCAGGCTAGTTCGATAAGCAATATAATCGCGCAGGCAACCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

162

Amino Acids

18.73

Weight (kDa)

7.66

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 1 - 140 3.1e-55 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 23, 177
Acc16I TGCGCA 1 cut(s) 411
AccII CGCG 2 cut(s) 397, 470
AciI CCGC 1 cut(s) 397
AcsI RAATTY 1 cut(s) 348
AgsI TTSAA 2 cut(s) 245, 371
AluBI AGCT 3 cut(s) 103, 136, 407
AluI AGCT 3 cut(s) 103, 136, 407
Alw26I GTCTC 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 2 cut(s) 288, 407
ApoI RAATTY 1 cut(s) 348
ArsI GACNNNNNNTTYG 4 cut(s) 222, 254, 266, 298
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 3 cut(s) 397, 412, 472
AspS9I GGNCC 2 cut(s) 128, 238
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 128, 238
BauI CACGAG 1 cut(s) 123
BbvI GCAGC 2 cut(s) 300, 394
BcgI CGANNNNNNTGC 2 cut(s) 448, 482
BciVI GTATCC 1 cut(s) 155
BclI TGATCA 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 189, 447
BfuI GTATCC 1 cut(s) 155
BisI GCNGC 2 cut(s) 289, 408
BlsI GCNGC 2 cut(s) 290, 409
Bme18I GGWCC 2 cut(s) 128, 238
BmgT120I GGNCC 2 cut(s) 128, 238
BmiI GGNNCC 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 349
BseNI ACTGG 1 cut(s) 345
BseXI GCAGC 2 cut(s) 300, 394
Bsh1236I CGCG 2 cut(s) 397, 470
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 277
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 329
Bsp143I GATC 2 cut(s) 214, 309
BspACI CCGC 1 cut(s) 397
BspANI GGCC 1 cut(s) 329
BspFNI CGCG 2 cut(s) 397, 470
BspLI GGNNCC 1 cut(s) 130
BsrI ACTGG 1 cut(s) 345
BssMI GATC 2 cut(s) 214, 309
BssSI CACGAG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 444, 474
BstDEI CTNAG 1 cut(s) 104
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 2 cut(s) 397, 470
BstHHI GCGC 3 cut(s) 397, 412, 472
BstKTI GATC 2 cut(s) 217, 312
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 2 cut(s) 214, 309
BstMCI CGRYCG 1 cut(s) 277
BstNSI RCATGY 1 cut(s) 208
BstUI CGCG 2 cut(s) 397, 470
BstV1I GCAGC 2 cut(s) 300, 394
BsuI GTATCC 1 cut(s) 155
BsuRI GGCC 1 cut(s) 329
BtsCI GGATG 1 cut(s) 349
Cac8I GCNNGC 2 cut(s) 444, 474
CaiI CAGNNNCTG 1 cut(s) 294
CfoI GCGC 3 cut(s) 397, 412, 472
Cfr13I GGNCC 2 cut(s) 128, 238
CseI GACGC 1 cut(s) 280
CviAII CATG 1 cut(s) 205
CviJI RGCY 5 cut(s) 103, 136, 329, 407, 446
CviKI_1 RGCY 5 cut(s) 103, 136, 329, 407, 446
DdeI CTNAG 1 cut(s) 104
DpnI GATC 2 cut(s) 216, 311
DpnII GATC 2 cut(s) 214, 309
Eco147I AGGCCT 1 cut(s) 329
Eco47I GGWCC 2 cut(s) 128, 238
EcoO109I RGGNCCY 2 cut(s) 128, 238
FaeI CATG 1 cut(s) 208
FaiI YATR 7 cut(s) 23, 177, 186, 206, 336, 338, 464
FaqI GGGAC 1 cut(s) 114
FatI CATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 2 cut(s) 289, 408
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 2 cut(s) 289, 408
FspBI CTAG 2 cut(s) 189, 447
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 3 cut(s) 396, 411, 471
GluI GCNGC 2 cut(s) 289, 408
HaeIII GGCC 1 cut(s) 329
HgaI GACGC 1 cut(s) 280
HhaI GCGC 3 cut(s) 397, 412, 472
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 3 cut(s) 395, 410, 470
HinP1I GCGC 3 cut(s) 395, 410, 470
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 6 cut(s) 4, 115, 250, 268, 374, 416
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 48, 365
Hpy188III TCNNGA 4 cut(s) 98, 218, 272, 371
Hpy8I GTNNAC 2 cut(s) 48, 365
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 3 cut(s) 122, 204, 434
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 3 cut(s) 395, 410, 470
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 2 cut(s) 214, 309
LpnPI CCDG 5 cut(s) 326, 345, 398, 428, 458
Lsp1109I GCAGC 2 cut(s) 300, 394
MaeI CTAG 2 cut(s) 189, 447
MalI GATC 2 cut(s) 216, 311
MboI GATC 2 cut(s) 214, 309
MboII GAAGA 2 cut(s) 86, 233
MluCI AATT 4 cut(s) 142, 230, 299, 348
MnlI CCTC 4 cut(s) 36, 119, 152, 229
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 1 cut(s) 315
MvnI CGCG 2 cut(s) 397, 470
NdeII GATC 2 cut(s) 214, 309
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 1 cut(s) 130
NsbI TGCGCA 1 cut(s) 411
NspI RCATGY 1 cut(s) 208
PceI AGGCCT 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 6 cut(s) 4, 115, 250, 268, 374, 416
PkrI GCNGC 2 cut(s) 290, 409
PpuMI RGGWCCY 2 cut(s) 128, 238
PsiI TTATAA 2 cut(s) 23, 177
Psp5II RGGWCCY 2 cut(s) 128, 238
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 2 cut(s) 128, 238
PspPPI RGGWCCY 2 cut(s) 128, 238
PstNI CAGNNNCTG 1 cut(s) 294
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 2 cut(s) 289, 408
Sau3AI GATC 2 cut(s) 214, 309
Sau96I GGNCC 2 cut(s) 128, 238
SetI ASST 7 cut(s) 47, 93, 105, 130, 138, 240, 409
SinI GGWCC 2 cut(s) 128, 238
Sse9I AATT 4 cut(s) 142, 230, 299, 348
SseBI AGGCCT 1 cut(s) 329
SsiI CCGC 1 cut(s) 397
SspMI CTAG 2 cut(s) 189, 447
StuI AGGCCT 1 cut(s) 329
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 273, 377, 452
TaqII GACCGA 1 cut(s) 291
TasI AATT 4 cut(s) 142, 230, 299, 348
TfiI GAWTC 6 cut(s) 4, 115, 250, 268, 374, 416
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TseI GCWGC 2 cut(s) 288, 407
TspDTI ATGAA 3 cut(s) 242, 360, 408
VpaK11BI GGWCC 2 cut(s) 128, 238
XapI RAATTY 1 cut(s) 348
XceI RCATGY 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 2 cut(s) 189, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.