MD05G1151900.v1.1

Ferric-chelate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005090
Physical Location & Seq
Reverse (-)
28262409 .. 28264949
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1151900.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGAAGGCATTCATAGGTTTCTTCATCGTCTTCATCTTTGGCTTATCAGCCACTGTGAATTCACAAACAGATTCCTGCAGCTCAACTCTTAACCTGCAGAATGTCAATCTGCCCTTTGATGCAGCTTCCCTCAATTGCTTTGCAGTTTGGGATGCTCACAACTACATCCTCAGATACTCACAGACCTTATCAAACCTATGGACCTTTGTCCTCTCAGCACCAGCTGCAAATTCATACATTGCAATTGGGTTCTCAAGCAATGGCCAGATGGTGGGGTCCAGTGCAATTGTGGGGTGGATGTCCCCAACAGGAGGAGAGATCAAGCAGTATTACTTGGGTGGGACCTCACCTAACCTTGTGGAGCCCAACAAGGGGAGCCTTCAAATTGGAAGCAACTTTTCCTTATTTACATCCCAGTCCAACAGAACGTACCTAGGGTTCCAATTGGAGACCAACCAGCCACTATCAAGGCTCATATACGCTGTTGGGCCAGATGGGTTGCTGCCTGTGGCTCCAAACTACAGATTGACTGAGCACAGTGACAAGGTGTCCACATCCATAAACTATATCACAGGTCAAGGTAAATCGAGCGCAGATAGTCCATACTCGAGATTGAGGAAGAGCCATGGAGTACTAAACATGCTAGGATGGGGCATTTTGATGATAATTGGAGTAATAATTGCTCGTGTTCTATGTGGATTTGTCTTGAATAATAAGCTCAATGCTGATGTCTCCGCCCACAAATCTCTTGGTATCTTCGTTCTTGTCCTTGGTTGTCTTCAGGTTATGGCTCTTTTGGTTCGACCGGAGAAGGAATCGAAGGTTCGAAAGTACTGGAATTGGTACCATCAAGGAGTGGGAAGGATTCTGATCATATTTGCAGTAGCAAATGTTTTCTATGGAATCCATTTGGGAGAAAAAGGGAAGGGATGGAGTGCTGGCTATGGAGTTGTTATTTCTATCTTATTTGTTACTGCTTTTATTTTGGAGTTAAGATTGTGGCTTAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DOMON

Protein Analysis

338

Amino Acids

36.97

Weight (kDa)

9.51

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOMON PF03351 67 - 123 2.8e-08 DOMON domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 102
Acc65I GGTACC 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 843
AccB7I CCANNNNNTGG 1 cut(s) 271
AciI CCGC 1 cut(s) 735
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 58, 229
AcuI CTGAAG 1 cut(s) 764
AfaI GTAC 4 cut(s) 431, 633, 833, 845
AfiI CCNNNNNNNGG 4 cut(s) 271, 311, 371, 372
AgsI TTSAA 2 cut(s) 383, 709
AhdI GACNNNNNGTC 1 cut(s) 547
AluBI AGCT 4 cut(s) 81, 125, 224, 718
AluI AGCT 4 cut(s) 81, 125, 224, 718
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 2 cut(s) 443, 736
AlwNI CAGNNNCTG 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 607
AoxI GGCC 2 cut(s) 262, 488
ApeKI GCWGC 4 cut(s) 78, 122, 224, 502
ApoI RAATTY 2 cut(s) 58, 229
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 843
AspA2I CCTAGG 1 cut(s) 433
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 4 cut(s) 201, 276, 342, 488
AsuHPI GGTGA 1 cut(s) 339
AsuII TTCGAA 1 cut(s) 826
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 3 cut(s) 201, 276, 342
AvrII CCTAGG 1 cut(s) 433
BalI TGGCCA 1 cut(s) 264
BanI GGYRCC 1 cut(s) 843
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 684
BbsI GAAGAC 2 cut(s) 22, 770
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 4 cut(s) 90, 134, 211, 489
BccI CCATC 5 cut(s) 262, 488, 642, 855, 924
BclI TGATCA 1 cut(s) 870
BcoDI GTCTC 2 cut(s) 443, 736
BfaI CTAG 2 cut(s) 434, 644
BfmI CTRYAG 3 cut(s) 76, 95, 520
BfuAI ACCTGC 1 cut(s) 102
BisI GCNGC 4 cut(s) 79, 123, 225, 503
BlnI CCTAGG 1 cut(s) 433
BlsI GCNGC 4 cut(s) 80, 124, 226, 504
BmcAI AGTACT 2 cut(s) 633, 833
Bme18I GGWCC 3 cut(s) 201, 276, 342
BmeRI GACNNNNNGTC 1 cut(s) 547
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 4 cut(s) 201, 276, 342, 488
BmiI GGNNCC 7 cut(s) 277, 343, 363, 377, 440, 513, 845
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 2 cut(s) 109, 142
BmuI ACTGGG 1 cut(s) 409
BoxI GACNNNNGTC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 22, 770
Bpu14I TTCGAA 1 cut(s) 826
BpuEI CTTGAG 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 869
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 433, 625, 769
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 311, 371, 372
Bse1I ACTGG 3 cut(s) 279, 415, 839
Bse3DI GCAATG 2 cut(s) 237, 265
Bse8I GATNNNNATC 1 cut(s) 869
BseDI CCNNGG 3 cut(s) 433, 625, 769
BseGI GGATG 7 cut(s) 157, 165, 303, 410, 554, 653, 935
BseJI GATNNNNATC 1 cut(s) 869
BseLI CCNNNNNNNGG 4 cut(s) 271, 311, 371, 372
BseMI GCAATG 2 cut(s) 237, 265
BseMII CTCAG 3 cut(s) 184, 228, 522
BseNI ACTGG 3 cut(s) 279, 415, 839
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 4 cut(s) 90, 134, 211, 489
Bsh1285I CGRYCG 1 cut(s) 806
BshFI GGCC 2 cut(s) 264, 490
BshNI GGYRCC 1 cut(s) 843
BsiEI CGRYCG 1 cut(s) 806
BsiHKAI GWGCWC 1 cut(s) 537
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 1 cut(s) 806
BslFI GGGAC 2 cut(s) 286, 355
BslI CCNNNNNNNGG 4 cut(s) 271, 311, 371, 372
BsmAI GTCTC 2 cut(s) 443, 736
BsmFI GGGAC 2 cut(s) 286, 355
BsmI GAATGC 1 cut(s) 8
BsnI GGCC 2 cut(s) 264, 490
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 607
Bsp119I TTCGAA 1 cut(s) 826
Bsp1286I GDGCHC 2 cut(s) 366, 537
Bsp143I GATC 2 cut(s) 318, 870
Bsp19I CCATGG 1 cut(s) 625
BspACI CCGC 1 cut(s) 735
BspANI GGCC 2 cut(s) 264, 490
BspCNI CTCAG 3 cut(s) 183, 227, 523
BspLI GGNNCC 7 cut(s) 277, 343, 363, 377, 440, 513, 845
BspMAI CTGCAG 2 cut(s) 80, 99
BspMI ACCTGC 1 cut(s) 102
BspQI GCTCTTC 1 cut(s) 614
BspT104I TTCGAA 1 cut(s) 826
BspT107I GGYRCC 1 cut(s) 843
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 2 cut(s) 237, 265
BsrI ACTGG 3 cut(s) 279, 415, 839
BssECI CCNNGG 3 cut(s) 433, 625, 769
BssMI GATC 2 cut(s) 318, 870
BssSI CACGAG 1 cut(s) 684
BssT1I CCWWGG 3 cut(s) 433, 625, 769
Bst2BI CACGAG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 55, 539
Bst6I CTCTTC 1 cut(s) 614
BstAPI GCANNNNNTGC 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 826
BstC8I GCNNGC 1 cut(s) 940
BstDEI CTNAG 4 cut(s) 170, 214, 531, 1004
BstDSI CCRYGG 1 cut(s) 625
BstF5I GGATG 7 cut(s) 157, 165, 303, 410, 554, 653, 935
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 2 cut(s) 321, 873
BstMAI GTCTC 2 cut(s) 443, 736
BstMBI GATC 2 cut(s) 318, 870
BstMCI CGRYCG 1 cut(s) 806
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 643
BstPAI GACNNNNGTC 1 cut(s) 206
BstSFI CTRYAG 3 cut(s) 76, 95, 520
BstV1I GCAGC 4 cut(s) 90, 134, 211, 489
BstV2I GAAGAC 2 cut(s) 22, 770
BsuRI GGCC 2 cut(s) 264, 490
BtgI CCRYGG 1 cut(s) 625
BtsCI GGATG 7 cut(s) 157, 165, 303, 410, 554, 653, 935
BtsIMutI CAGTG 3 cut(s) 51, 286, 544
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 940
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 1 cut(s) 593
Cfr13I GGNCC 4 cut(s) 201, 276, 342, 488
Csp6I GTAC 4 cut(s) 430, 632, 832, 844
CviAII CATG 2 cut(s) 626, 640
CviQI GTAC 4 cut(s) 430, 632, 832, 844
DdeI CTNAG 4 cut(s) 170, 214, 531, 1004
DpnI GATC 2 cut(s) 320, 872
DpnII GATC 2 cut(s) 318, 870
DriI GACNNNNNGTC 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 614
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 1 cut(s) 614
EciI GGCGGA 1 cut(s) 724
Eco130I CCWWGG 3 cut(s) 433, 625, 769
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 3 cut(s) 201, 276, 342
Eco57I CTGAAG 1 cut(s) 764
Eco88I CYCGRG 1 cut(s) 607
EcoO109I RGGNCCY 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 58
EcoT14I CCWWGG 3 cut(s) 433, 625, 769
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 433, 625, 769
FaeI CATG 2 cut(s) 629, 643
FaqI GGGAC 2 cut(s) 286, 355
FatI CATG 2 cut(s) 625, 639
FbaI TGATCA 1 cut(s) 870
Fnu4HI GCNGC 4 cut(s) 79, 123, 225, 503
FokI GGATG 7 cut(s) 152, 164, 310, 397, 541, 660, 942
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 79, 123, 225, 503
FspBI CTAG 2 cut(s) 434, 644
GlaI GCGC 1 cut(s) 592
GluI GCNGC 4 cut(s) 79, 123, 225, 503
HaeIII GGCC 2 cut(s) 264, 490
HapII CCGG 1 cut(s) 806
HhaI GCGC 1 cut(s) 593
Hin1II CATG 2 cut(s) 629, 643
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HinfI GANTC 4 cut(s) 71, 815, 865, 903
HpaII CCGG 1 cut(s) 806
HphI GGTGA 1 cut(s) 339
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 2 cut(s) 173, 870
Hpy188III TCNNGA 2 cut(s) 609, 706
Hpy8I GTNNAC 1 cut(s) 552
HpyAV CCTTC 5 cut(s) 389, 805, 814, 855, 919
HpyCH4III ACNGT 2 cut(s) 55, 539
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 8 cut(s) 78, 97, 122, 143, 227, 242, 284, 881
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 4 cut(s) 170, 214, 531, 1004
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 2 cut(s) 629, 643
HspAI GCGC 1 cut(s) 591
KpnI GGTACC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 870
Kzo9I GATC 2 cut(s) 318, 870
LguI GCTCTTC 1 cut(s) 614
LmnI GCTCC 3 cut(s) 361, 375, 517
Lsp1109I GCAGC 4 cut(s) 90, 134, 211, 489
LweI GCATC 2 cut(s) 109, 142
MaeI CTAG 2 cut(s) 434, 644
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 539, 970
MalI GATC 2 cut(s) 320, 872
MboI GATC 2 cut(s) 318, 870
MboII GAAGA 5 cut(s) 13, 22, 631, 748, 770
MfeI CAATTG 4 cut(s) 133, 243, 285, 443
MhlI GDGCHC 2 cut(s) 366, 537
MlsI TGGCCA 1 cut(s) 264
MluNI TGGCCA 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 6 cut(s) 140, 179, 221, 305, 355, 609
Mox20I TGGCCA 1 cut(s) 264
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 264
MseI TTAA 2 cut(s) 90, 992
MslI CAYNNNNRTG 1 cut(s) 659
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 1 cut(s) 806
MunI CAATTG 4 cut(s) 133, 243, 285, 443
Mva1269I GAATGC 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 224
NcoI CCATGG 1 cut(s) 625
NdeII GATC 2 cut(s) 318, 870
NlaIII CATG 2 cut(s) 629, 643
NlaIV GGNNCC 7 cut(s) 277, 343, 363, 377, 440, 513, 845
NmuCI GTSAC 1 cut(s) 539
NspI RCATGY 1 cut(s) 643
NspV TTCGAA 1 cut(s) 826
PaeR7I CTCGAG 1 cut(s) 607
PciSI GCTCTTC 1 cut(s) 614
PctI GAATGC 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 4 cut(s) 71, 815, 865, 903
PflMI CCANNNNNTGG 1 cut(s) 271
PkrI GCNGC 4 cut(s) 80, 124, 226, 504
PpuMI RGGWCCY 1 cut(s) 342
PshAI GACNNNNGTC 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 342
PspN4I GGNNCC 7 cut(s) 277, 343, 363, 377, 440, 513, 845
PspPI GGNCC 4 cut(s) 201, 276, 342, 488
PspPPI RGGWCCY 1 cut(s) 342
PsrI GAACNNNNNNTAC 2 cut(s) 422, 454
PstI CTGCAG 2 cut(s) 80, 99
PstNI CAGNNNCTG 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 4 cut(s) 431, 633, 833, 845
RsaNI GTAC 4 cut(s) 430, 632, 832, 844
RseI CAYNNNNRTG 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 614
SaqAI TTAA 2 cut(s) 90, 992
SatI GCNGC 4 cut(s) 79, 123, 225, 503
Sau3AI GATC 2 cut(s) 318, 870
Sau96I GGNCC 4 cut(s) 201, 276, 342, 488
ScaI AGTACT 2 cut(s) 633, 833
SduI GDGCHC 2 cut(s) 366, 537
SfaNI GCATC 2 cut(s) 109, 142
SfcI CTRYAG 3 cut(s) 76, 95, 520
Sfr274I CTCGAG 1 cut(s) 607
SfuI TTCGAA 1 cut(s) 826
SinI GGWCC 3 cut(s) 201, 276, 342
SlaI CTCGAG 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 253, 607
SmoI CTYRAG 2 cut(s) 253, 607
SsiI CCGC 1 cut(s) 735
SspMI CTAG 2 cut(s) 434, 644
StyI CCWWGG 3 cut(s) 433, 625, 769
TaaI ACNGT 2 cut(s) 55, 539
TaiI ACGT 1 cut(s) 431
TaqI TCGA 5 cut(s) 587, 608, 802, 818, 826
TatI WGTACW 2 cut(s) 631, 831
TfiI GAWTC 4 cut(s) 71, 815, 865, 903
Tru1I TTAA 2 cut(s) 90, 992
Tru9I TTAA 2 cut(s) 90, 992
TscAI CASTG 3 cut(s) 58, 286, 544
TseFI GTSAC 1 cut(s) 539
TseI GCWGC 4 cut(s) 78, 122, 224, 502
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 4 cut(s) 13, 17, 22, 222
TspRI CASTG 3 cut(s) 58, 286, 544
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 3 cut(s) 201, 276, 342
XapI RAATTY 2 cut(s) 58, 229
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 2 cut(s) 286, 746
XhoI CTCGAG 1 cut(s) 607
XmaJI CCTAGG 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 434, 644
ZrmI AGTACT 2 cut(s) 633, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.